IL120338A0 - Single step DNA fragments assembly - Google Patents
Single step DNA fragments assemblyInfo
- Publication number
- IL120338A0 IL120338A0 IL12033897A IL12033897A IL120338A0 IL 120338 A0 IL120338 A0 IL 120338A0 IL 12033897 A IL12033897 A IL 12033897A IL 12033897 A IL12033897 A IL 12033897A IL 120338 A0 IL120338 A0 IL 120338A0
- Authority
- IL
- Israel
- Prior art keywords
- dna fragments
- single step
- step dna
- fragments assembly
- assembly
- Prior art date
Links
- 239000012634 fragment Substances 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL12033897A IL120338A0 (en) | 1997-02-27 | 1997-02-27 | Single step DNA fragments assembly |
| PCT/IL1998/000095 WO1998038297A1 (en) | 1997-02-27 | 1998-02-26 | Single step assembly of multiple dna fragments |
| AU61125/98A AU6112598A (en) | 1997-02-27 | 1998-02-26 | Single step assembly of multiple dna fragments |
| PCT/IL1998/000097 WO1998038299A1 (en) | 1997-02-27 | 1998-02-26 | Single step assembly of multiple dna fragments |
| AU62287/98A AU6228798A (en) | 1997-02-27 | 1998-02-26 | Single step assembly of multiple dna fragments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL12033897A IL120338A0 (en) | 1997-02-27 | 1997-02-27 | Single step DNA fragments assembly |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL120338A0 true IL120338A0 (en) | 1997-06-10 |
Family
ID=11069866
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL12033897A IL120338A0 (en) | 1997-02-27 | 1997-02-27 | Single step DNA fragments assembly |
Country Status (3)
| Country | Link |
|---|---|
| AU (2) | AU6228798A (en) |
| IL (1) | IL120338A0 (en) |
| WO (2) | WO1998038299A1 (en) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6352842B1 (en) | 1995-12-07 | 2002-03-05 | Diversa Corporation | Exonucease-mediated gene assembly in directed evolution |
| US6713279B1 (en) | 1995-12-07 | 2004-03-30 | Diversa Corporation | Non-stochastic generation of genetic vaccines and enzymes |
| US6358709B1 (en) | 1995-12-07 | 2002-03-19 | Diversa Corporation | End selection in directed evolution |
| US7018793B1 (en) | 1995-12-07 | 2006-03-28 | Diversa Corporation | Combinatorial screening of mixed populations of organisms |
| US6740506B2 (en) | 1995-12-07 | 2004-05-25 | Diversa Corporation | End selection in directed evolution |
| US6361974B1 (en) | 1995-12-07 | 2002-03-26 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
| US5830696A (en) | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
| US6764835B2 (en) | 1995-12-07 | 2004-07-20 | Diversa Corporation | Saturation mutageneis in directed evolution |
| US6939689B2 (en) | 1995-12-07 | 2005-09-06 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
| US6537776B1 (en) | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
| US6562594B1 (en) | 1999-09-29 | 2003-05-13 | Diversa Corporation | Saturation mutagenesis in directed evolution |
| US6238884B1 (en) | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
| US6825011B1 (en) | 1998-12-17 | 2004-11-30 | Yuri Rumantichikov | Methods for insertion of nucleic acids into circular vectors |
| DE19736591A1 (en) * | 1997-08-22 | 1999-02-25 | Peter Prof Dr Hegemann | Preparing long nucleic acid polymers from linkable oligonucleotides |
| AU4039400A (en) * | 1999-03-26 | 2000-10-16 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
| CA2317865A1 (en) * | 2000-03-07 | 2001-09-07 | University Of Guelph | Dna joining method |
| EP1741787A1 (en) * | 2000-03-07 | 2007-01-10 | University Of Guelph | DNA joining method |
| AU2003227303A1 (en) | 2002-03-18 | 2003-09-29 | Yeda Research And Development Co. Ltd. | Methods of identifying gene expression products resultant from alternative splicing and methods of diagnosing and treating disorders associated therewith |
| FR2882063B1 (en) * | 2005-02-15 | 2007-06-22 | Univ Aix Marseille Ii | PROCESS FOR THE PREPARATION OF SYNTHETIC FRAGMENTS OF BICATENE NUCLEIC ACIDS |
| DE602006017405D1 (en) * | 2005-08-11 | 2010-11-18 | Synthetic Genomics Inc | PROCESS FOR IN VITRO RECOMBINATION |
| WO2007021944A2 (en) | 2005-08-11 | 2007-02-22 | The J. Craig Venter Institute | In vitro recombination method |
| CN101501207B (en) * | 2005-12-06 | 2014-03-12 | 合成基因组股份有限公司 | Synthetic genomes |
| US20070292954A1 (en) * | 2006-04-21 | 2007-12-20 | The Brigham And Women's Hospital, Inc. | Generation of recombinant DNA by sequence-and ligation-independent cloning |
| US8053191B2 (en) | 2006-08-31 | 2011-11-08 | Westend Asset Clearinghouse Company, Llc | Iterative nucleic acid assembly using activation of vector-encoded traits |
| EP3064599B1 (en) | 2008-02-15 | 2018-12-12 | Synthetic Genomics, Inc. | Methods for in vitro joining and combinatorial assembly of nucleic acid molecules |
| US9487808B2 (en) | 2010-09-24 | 2016-11-08 | Massachusetts Institute Of Technology | Rapid assembly of multiple arbitrary length DNA fragments |
| AU2011338841B2 (en) | 2010-11-12 | 2017-02-16 | Gen9, Inc. | Methods and devices for nucleic acids synthesis |
| EP4039363A1 (en) | 2010-11-12 | 2022-08-10 | Gen9, Inc. | Protein arrays and methods of using and making the same |
| IL302248A (en) | 2011-08-26 | 2023-06-01 | Gen9 Inc | Preparations and methods for high-fidelity assembly of nucleic acids |
| US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
| WO2013163263A2 (en) | 2012-04-24 | 2013-10-31 | Gen9, Inc. | Methods for sorting nucleic acids and multiplexed preparative in vitro cloning |
| US20150191719A1 (en) | 2012-06-25 | 2015-07-09 | Gen9, Inc. | Methods for Nucleic Acid Assembly and High Throughput Sequencing |
| CN102787114B (en) * | 2012-08-11 | 2015-06-17 | 复旦大学 | Efficient directional seamless DNA (deoxyribonucleic acid) segment connecting method |
| KR101417989B1 (en) * | 2013-02-08 | 2014-07-09 | 주식회사 엔지노믹스 | Method for regulating length of overhang of double stranded DNA |
| EP3450558B1 (en) | 2014-08-27 | 2020-09-30 | New England Biolabs, Inc. | Synthon formation |
| US9963687B2 (en) | 2014-08-27 | 2018-05-08 | New England Biolabs, Inc. | Fusion polymerase and method for using the same |
| CN106995813B (en) * | 2017-03-23 | 2020-06-16 | 山东大学 | New technology for direct cloning of large genome segment and DNA multi-molecule assembly |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0385410B1 (en) * | 1989-02-28 | 1996-10-02 | Canon Kabushiki Kaisha | Partially double-stranded oligonucleotide and method for forming oligonucleotide |
| US5580759A (en) * | 1994-02-03 | 1996-12-03 | Board Of Regents, The University Of Texas System | Construction of recombinant DNA by exonuclease recession |
-
1997
- 1997-02-27 IL IL12033897A patent/IL120338A0/en unknown
-
1998
- 1998-02-26 AU AU62287/98A patent/AU6228798A/en not_active Abandoned
- 1998-02-26 WO PCT/IL1998/000097 patent/WO1998038299A1/en not_active Ceased
- 1998-02-26 WO PCT/IL1998/000095 patent/WO1998038297A1/en not_active Ceased
- 1998-02-26 AU AU61125/98A patent/AU6112598A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO1998038299A1 (en) | 1998-09-03 |
| AU6228798A (en) | 1998-09-18 |
| WO1998038297A1 (en) | 1998-09-03 |
| AU6112598A (en) | 1998-09-18 |
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