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IE900217L - Process for the preparation of an insulin precursor - Google Patents

Process for the preparation of an insulin precursor

Info

Publication number
IE900217L
IE900217L IE900217A IE21790A IE900217L IE 900217 L IE900217 L IE 900217L IE 900217 A IE900217 A IE 900217A IE 21790 A IE21790 A IE 21790A IE 900217 L IE900217 L IE 900217L
Authority
IE
Ireland
Prior art keywords
insulin
acid
residue
amino
precursor
Prior art date
Application number
IE900217A
Original Assignee
Timothy Gerard Vaughan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Timothy Gerard Vaughan filed Critical Timothy Gerard Vaughan
Publication of IE900217L publication Critical patent/IE900217L/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/62Insulins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Endocrinology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Glass Compositions (AREA)
  • Steroid Compounds (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

An insulin precursor of the formula I <IMAGE> in which R1 = H, an amino-acid or peptide residue which can be eliminated chemically or enzymatically, R2 = OH or an amino-acid or peptide residue, X = a radical joining the insulin A and B chain, Y = residue of a genetically encodable amino acid, Z = residue of a genetically encodable amino acid, A1-A20 and B1-B29 = insulin peptide sequences which are non-mutated or mutated by exchange of one or more amino acids, is prepared by reacting a precursor in which the disulphide bridges between positions A6 and A11, A7 and B7, and A20 and B19, have not yet been formed, with excess mercaptan in the presence of an organic redox system or at least one organic compound which forms such an organic redox system under the reaction conditions. The particularly preferred organic redox system is the pair of compounds ascorbic acid + dehydroascorbic acid. The insulin precursor of the formula I can be converted into insulin either enzymatically or chemically by known techniques. [EP0381958A2]
IE900217A 1989-01-21 1990-01-19 Process for the preparation of an insulin precursor IE900217L (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3901719A DE3901719A1 (en) 1989-01-21 1989-01-21 METHOD FOR PRODUCING AN INSULIN PREVENTE

Publications (1)

Publication Number Publication Date
IE900217L true IE900217L (en) 1990-07-21

Family

ID=6372512

Family Applications (1)

Application Number Title Priority Date Filing Date
IE900217A IE900217L (en) 1989-01-21 1990-01-19 Process for the preparation of an insulin precursor

Country Status (17)

Country Link
EP (1) EP0381958B1 (en)
JP (1) JPH02233699A (en)
AT (1) ATE126808T1 (en)
AU (1) AU624894B2 (en)
CA (1) CA2008245A1 (en)
DE (2) DE3901719A1 (en)
DK (1) DK0381958T3 (en)
ES (1) ES2078249T3 (en)
FI (1) FI900296A7 (en)
GR (1) GR3017591T3 (en)
HU (1) HU208704B (en)
IE (1) IE900217L (en)
IL (1) IL93115A (en)
NO (1) NO178861C (en)
NZ (1) NZ232178A (en)
PT (1) PT92907B (en)
ZA (1) ZA90396B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3901718A1 (en) * 1989-01-21 1990-07-26 Hoechst Ag METHOD FOR RENATURING INCORRECT RECOMBINANT OF INSULIN PREFERRED
AU664021B2 (en) * 1990-09-05 1995-11-02 Natinco Nv Solubilization of proteins in active forms
DE59305396D1 (en) * 1992-12-02 1997-03-20 Hoechst Ag Process for obtaining proinsulin with correctly connected cystine bridges
ES2279510T5 (en) * 1994-12-29 2012-01-19 Ferring International Center S.A. HUMAN INSULIN GENERATION.
KR100253916B1 (en) * 1997-12-29 2000-05-01 김충환 Method for preparing human insulin precursor
AU2151401A (en) 1999-12-22 2001-07-03 Novo Nordisk A/S Method for extractive refolding of scrambled single-chain polypeptides

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ196609A (en) * 1980-03-27 1984-02-03 Lilly Co Eli Preparation of proinsulin-like insulin precursors
DE3501641A1 (en) * 1985-01-19 1986-07-24 Hoechst Ag, 6230 Frankfurt METHOD FOR OBTAINING INSULIN PRECURSORS FROM REACTION MIXTURES WHICH ARE INCLUDED IN THE FOLDING OF INSULIN PRECURSORS FROM THE CORRESPONDING S-SULPHONATES
DE3901718A1 (en) * 1989-01-21 1990-07-26 Hoechst Ag METHOD FOR RENATURING INCORRECT RECOMBINANT OF INSULIN PREFERRED

Also Published As

Publication number Publication date
FI900296A7 (en) 1990-07-22
AU4862190A (en) 1990-07-26
FI900296A0 (en) 1990-01-18
ZA90396B (en) 1990-09-26
JPH02233699A (en) 1990-09-17
DE3901719A1 (en) 1990-07-26
EP0381958A3 (en) 1991-07-24
HU208704B (en) 1993-12-28
GR3017591T3 (en) 1995-12-31
ATE126808T1 (en) 1995-09-15
ES2078249T3 (en) 1995-12-16
PT92907B (en) 1995-12-29
HUT54389A (en) 1991-02-28
HU900197D0 (en) 1990-03-28
DE59009540D1 (en) 1995-09-28
NZ232178A (en) 1991-10-25
DK0381958T3 (en) 1995-12-18
NO178861B (en) 1996-03-11
CA2008245A1 (en) 1990-07-21
AU624894B2 (en) 1992-06-25
IL93115A0 (en) 1990-11-05
NO900278L (en) 1990-07-23
NO900278D0 (en) 1990-01-19
NO178861C (en) 1996-06-19
PT92907A (en) 1990-07-31
IL93115A (en) 1995-07-31
EP0381958B1 (en) 1995-08-23
EP0381958A2 (en) 1990-08-16

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