IE893530L - A process for the preparation of an insulin precursor in¹Streptomycetes - Google Patents
A process for the preparation of an insulin precursor in¹StreptomycetesInfo
- Publication number
- IE893530L IE893530L IE893530A IE353089A IE893530L IE 893530 L IE893530 L IE 893530L IE 893530 A IE893530 A IE 893530A IE 353089 A IE353089 A IE 353089A IE 893530 L IE893530 L IE 893530L
- Authority
- IE
- Ireland
- Prior art keywords
- lys
- gene
- preparation
- insulin precursor
- chain
- Prior art date
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title abstract 4
- 102000004877 Insulin Human genes 0.000 title abstract 3
- 108090001061 Insulin Proteins 0.000 title abstract 3
- 229940125396 insulin Drugs 0.000 title abstract 2
- 239000002243 precursor Substances 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 108020001507 fusion proteins Proteins 0.000 abstract 2
- 102000037865 fusion proteins Human genes 0.000 abstract 2
- NVGBPTNZLWRQSY-UWVGGRQHSA-N Lys-Lys Chemical compound NCCCC[C@H](N)C(=O)N[C@H](C(O)=O)CCCCN NVGBPTNZLWRQSY-UWVGGRQHSA-N 0.000 abstract 1
- 108010076181 Proinsulin Proteins 0.000 abstract 1
- 241001655322 Streptomycetales Species 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 108010054155 lysyllysine Proteins 0.000 abstract 1
- 229950001790 tendamistat Drugs 0.000 abstract 1
- 108010037401 tendamistate Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Endocrinology (AREA)
- Microbiology (AREA)
- Diabetes (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A gene for a truncated proinsulin in which the insulin B chain is linked to the A chain only via Lys or Lys-Lys is coupled to the tendamistat gene, this gene construct is inserted into an expression vector, and the latter is used to transform a Streptomycetes host cell, with the result that the corresponding fusion protein is expressed and secreted. The fusion protein can easily be cleaved to give insulin precursors.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3837273A DE3837273A1 (en) | 1987-05-05 | 1988-11-03 | Process for the production of an insulin precursor in streptomyces |
| DE19893927449 DE3927449A1 (en) | 1989-08-19 | 1989-08-19 | New insulin fusion proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IE893530L true IE893530L (en) | 1990-05-03 |
Family
ID=25873834
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE893530A IE893530L (en) | 1988-11-03 | 1989-11-02 | A process for the preparation of an insulin precursor in¹Streptomycetes |
Country Status (18)
| Country | Link |
|---|---|
| EP (1) | EP0367163B1 (en) |
| JP (1) | JPH02219588A (en) |
| KR (1) | KR900008040A (en) |
| CN (1) | CN1042378A (en) |
| AT (1) | ATE132531T1 (en) |
| AU (1) | AU619054B2 (en) |
| CA (1) | CA2002062A1 (en) |
| DE (1) | DE58909556D1 (en) |
| DK (1) | DK546889A (en) |
| ES (1) | ES2081826T3 (en) |
| FI (1) | FI95600C (en) |
| GR (1) | GR3018738T3 (en) |
| HU (1) | HU209596B (en) |
| IE (1) | IE893530L (en) |
| IL (1) | IL92178A (en) |
| NO (1) | NO894363L (en) |
| NZ (1) | NZ231222A (en) |
| PT (1) | PT92177B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4012818A1 (en) | 1990-04-21 | 1991-10-24 | Hoechst Ag | METHOD FOR THE PRODUCTION OF FOREIGN PROTEINS IN STREPTOMYCETES |
| US5426036A (en) * | 1987-05-05 | 1995-06-20 | Hoechst Aktiengesellschaft | Processes for the preparation of foreign proteins in streptomycetes |
| DE3714866A1 (en) * | 1987-05-05 | 1988-11-24 | Hoechst Ag | METHOD FOR THE PRODUCTION OF FOREIGN PROTEINS IN STREPTOMYCETES |
| CZ283234B6 (en) | 1990-09-05 | 1998-02-18 | Hoechst Aktiengesellschaft | Hydrolysis process of pre-proinsulin amino acid chain |
| GB9513967D0 (en) * | 1995-07-08 | 1995-09-06 | Univ Leicester | Insulin |
| ES2570937T3 (en) | 2008-08-07 | 2016-05-23 | Biocon Ltd | A process for the preparation of insulin compounds |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3418274A1 (en) * | 1984-05-17 | 1985-11-21 | Hoechst Ag, 6230 Frankfurt | SIGNAL PEPTIDE FOR THE EXCRETION OF PEPTIDES IN STREPTOMYCETS |
| DK129385A (en) * | 1985-03-22 | 1986-09-23 | Novo Industri As | PEPTIDES AND PREPARATION THEREOF |
| DK437786D0 (en) * | 1986-09-12 | 1986-09-12 | Nordisk Gentofte | insulin precursors |
| DE3707150A1 (en) * | 1987-03-06 | 1988-09-15 | Hoechst Ag | TENDAMISTAT DERIVATIVES |
| DE3714866A1 (en) * | 1987-05-05 | 1988-11-24 | Hoechst Ag | METHOD FOR THE PRODUCTION OF FOREIGN PROTEINS IN STREPTOMYCETES |
-
1989
- 1989-10-28 AT AT89120056T patent/ATE132531T1/en not_active IP Right Cessation
- 1989-10-28 EP EP89120056A patent/EP0367163B1/en not_active Expired - Lifetime
- 1989-10-28 DE DE58909556T patent/DE58909556D1/en not_active Expired - Fee Related
- 1989-10-28 ES ES89120056T patent/ES2081826T3/en not_active Expired - Lifetime
- 1989-11-01 FI FI895184A patent/FI95600C/en not_active IP Right Cessation
- 1989-11-01 AU AU43950/89A patent/AU619054B2/en not_active Ceased
- 1989-11-01 IL IL9217889A patent/IL92178A/en not_active IP Right Cessation
- 1989-11-01 NZ NZ231222A patent/NZ231222A/en unknown
- 1989-11-02 KR KR1019890015844A patent/KR900008040A/en not_active Abandoned
- 1989-11-02 DK DK546889A patent/DK546889A/en not_active Application Discontinuation
- 1989-11-02 NO NO89894363A patent/NO894363L/en unknown
- 1989-11-02 PT PT92177A patent/PT92177B/en not_active IP Right Cessation
- 1989-11-02 HU HU895649A patent/HU209596B/en not_active IP Right Cessation
- 1989-11-02 CA CA002002062A patent/CA2002062A1/en not_active Abandoned
- 1989-11-02 JP JP1287282A patent/JPH02219588A/en active Pending
- 1989-11-02 IE IE893530A patent/IE893530L/en unknown
- 1989-11-03 CN CN89108320A patent/CN1042378A/en active Pending
-
1996
- 1996-01-19 GR GR960400123T patent/GR3018738T3/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE58909556D1 (en) | 1996-02-15 |
| HU209596B (en) | 1994-08-29 |
| EP0367163A2 (en) | 1990-05-09 |
| HUT53675A (en) | 1990-11-28 |
| FI895184A0 (en) | 1989-11-01 |
| EP0367163A3 (en) | 1991-03-06 |
| DK546889D0 (en) | 1989-11-02 |
| IL92178A (en) | 1995-11-27 |
| CN1042378A (en) | 1990-05-23 |
| ATE132531T1 (en) | 1996-01-15 |
| JPH02219588A (en) | 1990-09-03 |
| NZ231222A (en) | 1991-08-27 |
| AU4395089A (en) | 1990-05-10 |
| GR3018738T3 (en) | 1996-04-30 |
| FI95600C (en) | 1996-02-26 |
| CA2002062A1 (en) | 1990-05-03 |
| IL92178A0 (en) | 1990-07-26 |
| KR900008040A (en) | 1990-06-02 |
| HU895649D0 (en) | 1990-01-28 |
| NO894363L (en) | 1990-05-04 |
| EP0367163B1 (en) | 1996-01-03 |
| AU619054B2 (en) | 1992-01-16 |
| PT92177A (en) | 1990-05-31 |
| FI95600B (en) | 1995-11-15 |
| DK546889A (en) | 1990-05-04 |
| PT92177B (en) | 1995-06-30 |
| ES2081826T3 (en) | 1996-03-16 |
| NO894363D0 (en) | 1989-11-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IE812224L (en) | Interferon | |
| AU7067291A (en) | Hypodermic injection syringe provided with a plunger able to retract and contain the hypodermic needle after use | |
| AU3671889A (en) | Mini-proinsulin, its preparation and use | |
| EP0515516A4 (en) | Totally synthetic affinity reagents | |
| IL92178A0 (en) | Process for the preparation of an insulin precursor in streptomycetes | |
| NZ218247A (en) | Hybrid plasmid for use in producing human lysozyme and the protein so produced | |
| NZ239652A (en) | Hydrolysis of preproinsulin to insulin and analogues using clostripain and optionally carboxypeptidase | |
| GR3021040T3 (en) | Process for production of stranger protein in streptomycetes | |
| EP0427863A4 (en) | Dna coding for protein capable of combining with enhancer of alpha-fetoprotein gene | |
| DE68924222D1 (en) | Megakaryocytopoietin, its production and use. | |
| AU1090688A (en) | Synthetic porcine growth hormone | |
| AU2281288A (en) | A process for preparing zirconia stabilized with yttrium | |
| IE881338L (en) | A process for the preparation of foreign proteins in¹streptomycetes | |
| AU4854985A (en) | Novel hormones and methods for making, using and assaying same | |
| ATE270336T1 (en) | NISINE | |
| HU895664D0 (en) | Process for selective cleaving of fusion proteins | |
| AU3658489A (en) | Medicaments | |
| UA19109A (en) | Method for increasing antileukemic activity of cyclophosphan |