IE50531B1 - Mercaptoacyl derivatives of keto substituted proline and pipecolic acid - Google Patents

Description

MERCAPTOACYL DERIVATIVES OF KETO SUBSTITUTED PROLINE AND PIPECOLIC ACID This invention relates to keto derivatives of mercaptoacyl proline and pipecolic acid of formula I and salts thereof (I) II c / \ (H C) (CH.) 2,P ,2q — N - C-COOR Λ I H R and R^ are independently selected from hydrogen and lower alkyl provided that R2 is lower alkyl only if R^ is also lower alkyl.

R^ and R^ are independently selected from hydrogen, lower alkyl, lower alkylthio, -(CH_) -SH, and halo substituted lower alkyl. Ζ Π R^ is hydrogen, a hydrolyzably removable protecting group, a chemically removable protecting group, or when R^ and Rg are other than -(CH2)n-SH a sulfide of the formula II S-(CH) -C-C— Ν ς-cooR m is 2ero, one or two n is one, two or three p and q are each one or two provided that both are not two.

The asterisk in the above formula indicates a center of asymmetry in the ring. In the case of proline, i.e., p and q are both one, this center is in the L-configuration. In the case of pipecolic acid, i.e., one of p and q is two, this center is in the D,L or L-configuration.

Asymmetric centers can also be present in the mercaptoacyl sidechain depending upon the definition of Ry, R2 and Ry The products can accordingly exist in stereoisomeric forms or as racemic mixtures thereof. All of these are within the scope of the invention. The synthesis described below can utilize the racemate or one of the enantiomers as starting materials. When the racemic starting material is used in the synthesis procedure, the stereoisomers obtained in final product can be separated by conventional chromatographic or fractional crystallization methods. Preferably, if there is an asymmetric center in the mercaptoacyl sidechain, it is in the D-configuration.

This invention in its broadest aspects relates to the mercaptoacyl derivatives of proline and pipecolic acid having formula I above and fco salts thereof, to compositions containing such compounds and to the method for using such compounds as anti-hypertensive agents.

The term lower alkyl as used in defining the symbols R, R^, Rj, and Rj are straight or branched chain hydrocarbon radicals having up to seven carbons, for example, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, t-butyl, pentyl and isopentyl. The preferred lower alkyl groups are up to four carbons with methyl and ethyl being most preferred. Similarly, the terms lower alkoxy and lower alkylthio refer to such lower alkyl groups attached to an oxygen or sulfur.

The term halo substituted lower alkyl refers to such lower alkyl groups described above in which one or more hydrogens have been replaced by chloro, bromo or fluoro groups such as trifluoromethyl, pentafluoroethyl, 2,2,2-trichloroethyl, chloromethyl, and bromomethyl.

The term hydrolyzably removable protecting group employed in defining R4 refers to a group that can be removed by conventional hydrolysis or ammonolysis. Acyl groups of the formula θ are suitable for this purpose wherein can be lower alkyl of 1 to 7 carbons, lower alkyl substituted with one or more chloro, bromo or fluoro groups, -(CHj^-cycloalkyl wherein the term cycloalkyl refers to saturated rings of 3 to 7 carbon atoms, preferably cyclohexyl, an aryl group such as R, , a hetero group such as -(CHj)^ '6 wherein r is zero, one, two or three; Rg is hydrogen, lower alkyl of 1 to 4 carbons, especially methyl, lower alkoxy of 1 to 4 carbons, especially methoxy, lower alkylthio of 1 to 4 carbons, especially methylthio, chloro, bromo, fluoro, trifluoromethyl, or hydroxy; and X is oxygen or sulfur. Preferred groups are the lower alkanoyl groups having up to four carbons, especially acetyl, and benzoyl.

The term chemically removable protecting group employed in defining R^ refers to groups such as p-methoxy, p-methoxybenzylcarbonyl, trityl, and t-butoxycarbonyl. These groups can be removed after the completion of the acylation reaction by various means such as by treatment with trifluoroacetic acid and anisole.

Preferred compounds of formula I are the L-proline containing derivative, i.e., p and q are both one, and R is hydrogen.

With respect to the mercaptoacyl sidechain, preferred as final products are those compounds wherein R^ is hydrogen; m is zero or one; R^ is hydrogen; and R^ and R2 are both lower alkyl of 1 to 4 carbons, especially both methyl, or Rj is hydrogen and R^ is hydrogen, lower alkyl of 1 to 4 carbons, especially methyl, trifluoromethyl, methylthio, or mercaptomethyl. Also preferred as both intermediates and final products are the above sidechains wherein R^ is lower alkanoyl of 1 to 4 carbons, especially acetyl, or benzoyl.

Especially preferred as final products are the compounds of formula I having the mercaptoacyl sidechain wherein R^ is hydrogen; m is one; R^ and R2 are hydrogen; R^ is methyl or hydrogen; and when R^ is methyl and R? is H,the asymmetric carbon atom to which R^ is attached is in the D-conficuration.

The compounds of formula X are obtained by coupling a keto-substituted proline or pipecolic acid of the formula (II) II c / \ 2( p ( 2 g HN- C*COOR I H with an acid or a reactive derivative thereof of the formula (III) Rl-S-(CH)— C — COOH 4 m , wherein R^ is hydrogen, a hydrolyzably or chemically removable protecting group to yield the product of the formula (IV) Rl-S-(CH) C — CO — N4 · m , (H2?>p (CH.) . 2 5 qpCOOR I H This reaction can be effected in the presence of a coupling agent such as dicyclohexylcarbodiimide or the acid can be activated by formation of its mixed anhydride, symmetrical anhydride, acid halide, active ester or use of Woodward reagent K, or N-ethoxycarbonyl-2-ethoxy-l,2-dihydroquinoline For a review of the methods or acylation, see Methoden der Organishchen Chemie (Houben-Weyl), Vol. XV, part II, page 1 et seq. (1974). Preferably, the acid halide, especially the acid chloride, of formula III is reacted with the acid of formula II.

If the proline or pipecolic acid of formula II is reacted in the ester form the resulting ester prod30 uet can be converted to the free acid, i.e., R is hydrogen, by 531 conventional means. For example, if R is ethyl this ester protecting group can be removed by saponification.

The product of formula IV is preferably isolated and purified by crystallization, e.g., by forming the dicyclohexylamine salt and then converting the salt to the free acid form by treatment with an aqueous solution of an acid, such as potassium acid sulfate.

The product of formula IV bearing the acyl group R_ -CO- can be. converted to the products of formula I wherein R^ is hydrogen by conventional hydrolysis or by ammonolysis.

The products of formula I wherein R^ and R, are other than - (CH.,) -SH and R. is j 4 n 4 II ^C\ R, R, (CH,) i3 i1 2l Ρ ι 2 5 -S-(CH)m CO _ N C* COOH I H are obtained by directly oxidizing with iodine a product of formula I wherein R4 is hydrogen.

The esters of formula I wherein R is lower alkyl can be obtained from the carboxylic acid compounds, i.e., wherein R is hydrogen, by conventional esterification procedures, e.g., by esterification with a diazoalkane such as diazomethane or a 1-alkyl3-p-tolyltriazene, such as l-n-butyl-3-p-tolyltriazene.

The compounds of Formula I can also be prepared by deprotecting, according to conventional methods, a keto-substituted proline or pipecolic acid product of Formula I having the keto-function protected by the conventional protecting groups as for example, a ketal protecting group, e.g., a dialkoxy ketal, as for example.

(V) H R.— S— (CH) —C—CO—N4 m j which can be deprotected by hydrolysis, as with an aqueous solution of hydrochloric acid at room temperature.

The compounds of Formula V can be prepared by starting with an N-carbobenzyloxy keto substituted proline or pipecolic acid of Formula II which is then treated with methanol in the presence of trimethyl orthoformate and concentrated sulfuric acid and the N-protecting group is then removed by hydrogenolysis in the presence of a palladium carbon catalyst to yield the dimethoxy intermediate of the formula CH, H C ...

(VI) <H2?>p Wq HN-C-COOR I * 531 - 10 The intermediate of formula VI is then coupled with an acid or a reactive derivative thereof of formula III to yield the dimethoxy compounds of formula V.

Reference is also made to the following publications for additional illustrative methodology for producing starting materials and intermediates: U.S.

Patents 4,046,889, 4,105,776, 4,154,935 and 4,116,962; Can. J. Biochem. & Physiol. 37, 583-587, (1959); JACS 79, p. 185-192, (1957); JACS 79, p. 192-197, (1957); Bull. Soc. Chem., 1965(8), p. 2253-2259; and Aus. J.

Chem. 20, p. 1493-15.09, (1967).

The procedures illustrated therein can be utilized as general methods for the synthesis of compounds and separation of isomers which can be utilized in the invention described in this application. Additional illustrative details are found in the examples which serve as models for the preparation of other members of the group.

The compounds of this invention form basic salts with a variety of inorganic or-organic bases.

The salt forming ion derived from such bases can be metal ions, e.g., aluminum, alkali metal ions, such as sodium or potassium, alkaline earth metal ions such as calcium or magnesium, or an amine salt ion, of which a number are known for this purpose, for example aralkylamines like dibenzylamine, Ν,Ν-dibenzylethylenediamine, lower alkylamines like methylamine, t-butylamine, procaine, lower alkylpiperidines like N-ethylpiperidine, cycloalkylamines, like cyclohexylamine or dicyclohexylamine, 150531 - 11 adamantanamine, benzathine, or salts derived from amino acids like arginine and lysine. The physiologically acceptable salts like the sodium or potassium salts can be used medicinally as described below and are preferred. These and other salts which are not necessarily physiologically acceptable are useful in isolating or purifying a product acceptable for the purposes described below, as illustrated with the dicyclohexylamine salt in the examples. The salts are produced by reacting the acid form of the compound with a equivalent of the base supplying the desired basic ion in a medium in which the salt precipitates or in aqueous medium and then lyophilizing. The free acid form can be obtained from the salt by conventional neutralization techniques, e.g., with potassium bisulfate, hydrochloric acid, etc.

The compound of formula I wherein R. is ’ hydrogen, ji , or the disulfide type substituent, R5-cespecially wherein R^ is hydrogen, are useful as hypotensive agents. They inhibit the conversion of the decapeptide angiotensin I to angiotensin II and, therefore, are useful in relieving angiotensin related hypertension. The action of the enzyme renin on angiotensinogen, a pseudoglobulin in blood plasma, produces angiotensin I. Angiotensin I is converted by angiotensin converting enzyme (ACE) to angiotensin II. The latter is an active pressor substance which has been implicated as the - 12 causative agent in various forms of hypertension in various manmalian species, e.g., rats and dogs.

The compounds of this invention intervene in the angiotensinogen (renin^ angiotensin I (ACE) angiotensin II sequence by inhibiting angiotensin converting enzyme and reducing or eliminating the formation of the pressor substance angiotensin II.

Thus by the administration of a composition containing one, or a combination of compounds of formula I angiotensin dependent hypertension in the species of mammal suffering therefrom is alleviated. A single dose, or preferably two to four divided daily doses, provided on a basis of about 0.1 to 100 mg. per kilogram of body weight per day, preferably about 1 to 15 mg. per kilogram of body weight per day is appropriate to reduce blood pressure. The substance is preferably administered orally, but parenteral routes such as the subcutaneous, intramuscular, intravenous or intraperitoneal routes can also be employed.

The compounds of this invention can also be formulated in combination with a diuretic for the treatment of hypertension. A combination product comprising a compound of this invention and a diuretic can be administered in an effective amount which comprises (for a 70 kg. mammal) a total daily dosage of about 30 to 600 mg., preferably about 30 to 300 mg., of a compound of this invention, and about 15 to 300 mg., preferably about 15 to 200 mg. of the diuretic, to a mammalian species in need - 13 thereof. Exemplary of the diuretics contemplated for use in combination with a compound of this invention are the thiazide diuretics, e.g., chlorthiazide,hydrochlorothiazide, flumethiazide, hydroglumethiazide,benzdroflumethiazide, methchlothiazide, trichlormethiazide, polythiazide or benzthiazide, as well as ethacrynic acid, ticrynafen, chlorthalidone, furodemide, musolintine, bumetanide, triamterene, amiloride and spironolactone, and salts of such compounds.

The compounds of formula X can be formulated for use in the reduction of blood pressure in compositions such as tablets, capsules or elixirs for oral administration or in sterile solutions or suspensions for parenteral administration. About 10 to 500 mg. of a compound or mixture of compounds of formula I is compounded with a physiologically acceptble vehicle, carrier, excipient, binder, preservative, stabilizer, flavor, etc., in a unit dosage form as called for by accepted pharmaceutical practice. The amount of active substance in these compositions or preparations is such that a suitable dosage in the range indicated is obtained.

Illustrative process details are set forth in the following examples for the various reactions. These examples are preferred embodiments and also serve as models for the preparation of other compounds of this invention. The temperatures are given in degrees on the centigrade scale. * - 14 Example 1 1-[3-(Acetyl thio )-1-oxopropyl]-4-oxo-L-proline a) N-Carbobenzyloxy-4-hydroxy-L-proline 26.5 g. (0.02 mole) of 4-hydroxy-L-proline and 32.8 ml. (0.23 mole) of benzyl chloroformate are reacted in 200 ml. of water and 100 ml. of acetone in the presence of 20 g. (0.02 mole) of potassium bicarbonate and 69.2 g. (0.50 mole) of potassium carbonate and worked up with 90 ml. of concentrated hydrochloric acid as described in Can.J. Biochem. & Physiol. 37, 584 (1959) to obtain N-carbobenzyloxy-4-hydroxy-L-proline. This product is reacted with cyclohexylamine to form the cyclohexylamine salt yield 69 g., m.p. 193-195°. The salt (34 g.) is neutralized with N-hydrochloric acid to obtain g. of free acid as a colorless glass [α]θ -70°, (c, 1% in chloroform). b) N-carbobenzyloxy^-keto-L-proline 21.5 g. (0.81 mole) of N-carbobenzyloxy-420 hydroxy-L-proline is oxidized in 1.2 liters of acetone with 83 ml. of 8N chromic acid in sulfuric acid as described in J.A.C.S. 79, 189 (1957). In order to facilitate the subsequent filtration of chromium salts, 30 g. of Celite (diatomaceous earth) is added to the acetone solution before introduction of the oxidizing agent. An air stirrer is employed. The reaction mixture is filtered and the acetone filtrate is concentrated to approximately 300 ml. before diluting with 1 liter of chloroform.

The solution is washed with 300 ml. of saturated - 15 sodium chloride (four times), dried (MgSO^), filtered and the solvent evaporated to give N-carbobenzyloxy-4-keto-L-proline (22.8 g.) which is crystallized from ether (50 ml.)—hexane (150 ml.) to obtain 17.2 g. (81%) of product, m.p. 99-101°, (“)p6 +17° (c,l% in chloroform). c) 4-Keto-L-proline, hydrobromide To 4.0 g. (0.015 mole) of N-carbobenzyloxy-4keto-L-proline are added 20 ml. of hydrogen bromide in acetic acid (30-32%). The mixture is frequently swirled over a period of eight minutes. At the end of this period (effervescence has stopped), the yellow-orange solution is layered over with 250 ml. of ether, triturating the gummy product. The ether is discarded and the resulting tacky solid is triturated with fresh ether and finally with 50 ml. of acetonitrile to give 4-keto-L-proline, hydrobromide as a crystalline solid weighing 2.7 g. (85%), m.p. 153-155° (dec.,), [α]θ6 -49° (c, 1% in water). d) 1-(3-(Acetylthio)-1-oxopropyl)-4-oxo-L-proline A stirred solution of 4.1 g. (0.0195 mole) of 4-keto-L-proline, hydrobromide in 50 ml. of water is cooled to 5° and treated portionwise with solid sodium carbonate (foaming is controlled by adding a few drops of ether) to pH 8.0 (approx. 2 g. required). Then while continuing stirring and cooling, a solution of 3.5 g. (0.012 mole) of 3-acetylthiopropionyl chloride in 5 ml. of ethyl acetate is added portionwise by means of a pipette while maintaining the pH at 7.0 - 8.0 by dropwise addition - 16 of 25% (w/v) sodium carbonate solution (about 10 ml.). After about 10 minutes the pH stabilizes at 8.0 8.4. After continued stirring and cooling for a total of 1 hour, the solution is washed with ethyl acetate (2 x 50 ml.), layered over with 50 ml. of ethyl acetate, stirred, cooled, acidified carefully with concentrated hydrochloric acid to pH 2.0, saturated with sodium chloride, and the layers are separated.

The aqueous phase is extracted with additional ethyl acetate (3 x 50 ml.), the combined organic layers dried (MgSO^) and the solvent evaporated, finally at 0.2 mm. to give 4.8 g. of a yellow-orange glass-like residue. This residue is dissolved in 35 ml. of ethyl acetate and treated with a solution of 3.5 g. of dicyclohexylamine in 5 ml. of ethyl acetate. On seeding and rubbing, crystalline 1-[3-(acetylthio-1oxopropyl)]-4-oxo-L-proline dicyclohexylamine salt separates, weight after cooling overnight, 2.7 g. (nearly colorless), m.p. 191-193° (dec.), [a]^ -24° (c, IS in CHC1 ).

This dicyclohexylamine salt is converted to the free acid by suspending it in ethyl acetate and treating with 45 ml. of 10% potassium bisulfate and stirring until two layers are obtained. After separa25 ting, the aqueous phase is extracted with ethyl acetate (4 x 75 ml.), the organic layers are combined, dried (MgSO^) and the solvent is evaporated to give 3.7 g. of light yellow glass-like material.

Anal. Calc'd. for C,„H,,NO,S: 13 5 - 17 C, 46.32; Η, 5.05; N, 5.40; S, 12.37; Found: C, 47.05; H, 5.50; N, 5.18; S, 12.07.

Example 2 1-(3-Mercapto-l-axopropyl)-4-oxo-L-proline Argon is passed through a cold solution of ml. of concentrated ammonium hydroxide in 22 ml. of water for 30 minutes. This material is then added while cooling and under a blanket of argon to 3.65 g. (0.014 mole) of 1-[3-(acetylthio)-loxopropyl]-4-oxo-L-proline. Approximately 30 minutes are required to dissolve the proline starting with the aid of a magnetic stirrer.

Stirring under argon is continued at room temperature for an additional two hours after which the solution is extracted with 30 ml. of ethyl acetate (this and subsequent operations are carried out as much as possible under an argon atmosphere). The aqueous layer is cooled, stirred, layered over with 30 ml. of ethyl acetate, and acidified portionwise with 1:1 HCl. Sodium chloride is added, the layers are separated, and the aqueous phase is extracted with additional ethyl acetate (3 x 30 ml.). A brown gummy fraction remains insoluble in either phase. After drying over MgSO^ the combined ethyl acetate layers are evaporated to give a mostly solid residue which becomes an amorphous solid when triturated with ether and the evaporation repeated to yield 1.4 g. of pale yellow material. 1.3 g. of this material is rubbed under 50 ml. of ether, cooled under argon for one hour. 0531 - 18 filtered, washed with ether, and dried in vacuo to yield 1.0 g. of 1-(3-mercapto-l-oxopropyl)-4-oxo-Xr-prolins, 0.67 (methanol on silica gel. visualized in iodine vapor). Anal. Calc'd. for CgH^NC^S ' 0.5 H2O: C, 42.46: H, 5.35; N, 6.19; S, 13.71 Found: C, 42.66; H, 5.39; N, 6.30; S, 13.30.

Example 3 (S)-l-((3-Acetylthio)-2-methyl-l-oxopropyl)-4-oxoL-proline Following the procedure of Example 1 but substituting D-3-acetylthio-2-methylpropionyl chloride for the 3-acetylthiopropionyl chloride, one obtains (S)-l-[(3-acetylthio)-2-methyl-l-oxopropyl]-4-oxo-L-proline.

Example 4 (S)-l-(3-Mercapto-2-methyl-l-oxopropyl)-4-oxo-Lproline The product from Example 3 is treated with ammonium hydroxide according to the procedure of Example 2 to yield (S)-1-(3-mercapto-2-methyl-1oxopropy1)-4-oxo-L-proline.

Example 5 1-f(3-Acetylthio)-2-trifluoromethyl-l-oxopropyl]-4oxo-L-proline a) D,L-3-(Acetylthio)-2-trifluoromethylpropionic acid α-Trifluoromethyl acrylic acid (10 g., 0.071 mole) [prepared according to the procedure set forth in J, Chem. Soc., 1954, p. 371] is cooled in a salt-ice-water bath, stirred and treated portionwise with 5.7 ml.(0.075 mole) of 97% thiolacetic - 19 acid. After the addition, the yellow liquid is stirred in the cold for one hour, allowed to warm to room temperature, and distilled to yield 14 g. (91%) of D,L-3-(acetylthio)-2-trifluoromethylpropionic acid as a light yellow oil, b.p. 149-153°/13mm. The material solidifies on storing in the cold. b) D,L-3- (Acetylthio)-2-trifluoromethylpropionyl chloride The D,L-3-(acetylthio)-2-trifluoromethylpropionic acid (7 g., 0.032 mole) is treated with 18 ml. (0.25) of redistilled thionyl chloride and the mixture is refluxed for three hours. After removing the excess thionyl chloride on a rotary evaporator, the residue is distilled to give 6.8 g. of D,L-3-(acetylthio)-2-trifluoromethylpropionyl chloride as a pale yellow oil; b.p. 80-82°/16 mm. c) 1-[(3-Acetylthio)-2-trifluoromethyl-l-oxopropyl]4-oxo-L-proline The D,I.-3- (acetylthio)-2-trif luoromethylpropionyl chloride is reacted with 4-keto-L-proline, hydrobromide according to the procedure of Example 1 (d) to yield 1-[(3-acetylthio)-2-trifluoromethyl-l-oxopropyl]-4-oxo-l-proline. This mixture of diastereoisomers can then be separated by conventional techniques.

Example 6 1-(3-Mercapto-2-trifluoromethyl-I-oxopropyl)-4oxo-L-proline The product from Example 5 (in the form of the diastereoisomer mixture or as the separated - 20 isomers) is hydrolyzed with concentrated ammonia according to the procedure of Example 2 to yield 1(3-mercapto-2-trifluoromethyl-1-oxopropyl)-4-oxoL-proline.

Example 7 (S)-1-(3-Mercapto-2-mercaptomethyl-1-oxopropyl)-4oxo-L-proline a) (S)-l-[3-(Acetylthio)-2-(acetylthiomethyl)-loxopropyl]-4-oxo-L-proline Following the procedure of Example 1 but substituting D-2-acetylthiomethyl-3-acetylthiopropionyl chloride for the 3-acetylthiopropionyl chloride in part (d) , one obtains (S)-l-[3-(acetylthio)-2(acetylthiomethyl)-1-oxopropyl]-4-oxo-L-proline. b) (5)-1-(3-Mercapto-2-mercaptomethyl-l-oxopropyl)-4-oxo-L-proline The product from part (a) is hdyrolyzed with concentrated ammonia according to the procedure of Example 2 to yield (S)-1-(3-mercapto-2-mercapto20 methyl-l-oxopropyl)-4-oxo-L-proline .

Example 8 1-(3-Mercapto-2-methylthio-l-oxopropyl)-4-oxo-Lproline a) 3-(Acetylthio)-2-(methylthioIpropionic acid 25 12.5 g. (0.094 mole) of methyl-2-(methylthio) acrylate [prepared from methyl 2-chloroacrylate according to the procedure of Gundesmann et al., Chemische Berichte 94, 3254 (1916)] is stirred with IN aqueous sodium hydroxide (94 ml.) with ice cooling. The mixture is allowed to warm to ambient temperature, then stirred for five hours. The S053 1 - 21 resulting solution is washed with ether, then acidified to pH 2 with concentrated hydrochloric acid.

The solid precipitate is extracted into methylene chloride, and the solution is washed with saturated sodium chloride and the solvent evaporated. The solid residue, 2-(methylthio)acrylic acid; m.p. 70-75°, is used immediately in the following reaction.

Equimolar amounts of 2-(methylthio)acrylic acid and thiolacetic acid are mixed under argon and stirred at 80° for several hours to yield 3-(acetylthio) -2- (methylthio)propionic acid. b) 3-(Acetylthio)-2-(methylthio)propionic acid chloride The 3-(acetylthio)-2-(methylthio)propionic acid is refluxed in thionyl chloride for two hours. The reaction mixture is distilled to remove excess thionyl chloride and the product is distilled in vacuo to yield 3-(acetylthio)-2-(methylthio) propionic acid chloride. c) 1-[3-(Acetylthio)-2-methylthio-l-oxopropyl]-4oxo-L-proline The 3-(acetylthio)-2-(methylthio)propionic acid chloride is reacted with 4-keto-L-proline, hydrobromide according to the procedure of Example 1 (d) to yield 1-[3-(acetylthio)-2-methylthio-l-oxopropyl ]-4-oxo-L-proline. d) 1-(3-Mercapto-2-methylthio-l-oxopropyl)-4oxo-L-proline The product from part (c) is hydrolyzed with - 22 concentrated ammonia according to the procedure of Example 2 to yield 1-(3-mercapto-2-methylthio-loxopropyl)-4-oxo-L-proline.

Example 9 1-(4-Mercapto-l-oxobutyl)-4-oxo-L-proline a) 1-[4-(Acetylthio)-l-oxobutyl]-4-oxo-L-proline Following the procedure of Example 1 but substituting 4-acetylthiobutyr yl chloride for the 3-acetylthiopropionyl chloride in part (d), one obtains 1-[4-(acetylthio)-1-oxobutyl]-4-oxo-Lproline. b) 1-(4-Mercapto-l-oxobutyl)-4-oxo-L-proline The product form part (a) is hydrolyzed with concentrated ammonia according to the procedure of Example 2 to yield 1-(4-mercapto-l-oxobutyl)-4oxo-L-proline.

Example 10 1-(2-Mercapto-l-oxoethyl) -4-oxo-L-prol'ine a) 1-[2-(Acetylthio)-1-oxoethyl]-4-oxo-L-proline Following the procedure of Example 1 but substituting acetylthioacetyl chloride for the 3-acetylthiopropionyl chloride in part (d), one obtains 1-[2-(acetylthio)-1-oxoethyl]-4-oxo-Lproline. b) 1-(2-Mercapto-l-oxoethyl)-4-oxo-L-proline The product from part (a) is hydrolyzed with concentrated ammonia according to the procedure of Example 2 to yield 1-(2-mercapto-l-oxoethyl)-4oxo-L-proline. - 23 Example 11 1-(3-Mercapto-2,2-dimethyl-l-oxopropyl)-4-oxo-Lproline a) 1-[3- (Acethylthio)-2,2-dimethy1-1-oxopropyl]5 4-oxo-L-proline Following the procedure of Example 1 but substituting 3-acetylthio-2,2-dimethylpropionyl chloride for the 3-acetylthiopropionyl chloride in part (d), one obtains 1-[3-(acetylthio)-2,210 dimethy1-1-oxopropyl]-4-oxo-L-proline. b) 1- (3-Mercapto-2,2-dimethyl-l-oxopropyl)-4oxo-L-proline The product from part (a) is hydrolyzed with concentrated ammonia according to the procedure of Example 2 to yield 1-(3-mercapto-2,2-dimethyl-loxopropyl)-4-oxo-L-proline.

Example 12 (S) —1— (3-Mercapto-2-ethy1-1-oxopropyl)-4-oxo-Lproline 20 a) (S)-l-(3-(Acetylthio)-2-ethyl-l-oxopropyl]-4oxo-L-proline Following the procedure of Example 1 but substituting D-3-acetylthio-2-ethylpropionyl chloride for the D-3-acetylthio-2-methylpropionyl chloride in part (d), one obtains (S)-l-[3-(acetylthio)-2ethyl-l-oxopropyl]-4-oxo-L-proline. b) (S) —1—(3-Mercapto-2-ethyl-l-oxopropyl)-4L-proline The product from part (a) is hydrolyzed with concentrated ammonia according to the procedure of - 24 Example 2 to yield (S)-l-(3-mercapto-2-ethyl-loxopropyl)-4-oxo-L-proline.

Example 13 1-(3-Mercapto-l-oxopropyl)-4-oxo-L-pipecolic acid 5 a) 1-[3-(Acetylthio)-1-oxopropyl]-4-oxo-L-pipecolic acid Following the procedure of Example 1 but substituting 4-keto-L-pipecolic acid for the 4keto-L-proline in part (d), one obtains 1-[3-(acetyl10 thio)-1-oxopropyl]-4-oxo-L-pipecolic acid. b) 1-(3-Mercapto-l-oxopropyl)-4-oxo-L-pipecolic acid The product from part (a) is hydrolyzed with concentrated ammonia to yield 1-(3-mercapto-loxopropyl)-4-oxo-L-pipecolic acid.

Example 14 (S) —1—(3-Mercapto-2-methyl-l-oxopropyl)-5-oxo-Lpipecolic acid a) (S)-1-[3-(Acetylthio)-2-methyl-l-oxopropyl]-5oxo-L-pipecolic acid Following the procedure of Example 1 but substituting 5-keto-L-pipecolic acid for the 4-keto-L-proline in part (d) and D-3-acetylthio-2methylpropionyl chloride for the acetylthiopropionyl chloride in part (d), one obtains (S)-1-[3-(acetyl25 thio-2-methyl-l-oxopropyl]-5-keto-L-pipecolic acid. b) (S)-l-(3-Mercapto-2-methyl-l-oxopropyl)-5keto-L-pipecolic acid The product from part (a) is hydrolyzed with concentrated ammonia according to the procedure of Example 2 to yield (S)-1-(3-mercapto-2-methyl-l50531 - 25 oxopropyl)-5-keto-L-pipecolic acid.

Example 15 1-(3-Mercapto-2-trifluoromethy1-1-oxopropy1)-4oxo-L-proline a) 3-(((4-Methoxy)phenylmethyl]thio]-2-trifluoromethylpropionyl chloride A neat mixture of 1-trifluoromethylacrylic acid (3.9 g.) and 4-methoxybenzylthiol (4.3 g.) is stirred at 100-110° for one hour. The mixture is allowed to cool to room temperature and the solid is recrystallized from cyclohexane to yield 3-(((4methoxy)phenyImethyl]thio]-2-trifluoromethyIpropionic acid; m.p. 72-74°.

Treatment of this acid with thionyl chloride yields 3-[[(4-methoxy)phenylmethyl]thio]-2-trifluoromethylpropionyl chloride. b) 1-[3-[[(4-Methoxy)phenylmethyl]thio]-2-trifluoromethyl-l-oxopropyl]-4-oxo-L-proline The 3-(((4-methoxy)phenylmethy1]thio]-220 trifluoromethylpropionyl chloride from part (a) is reacted with 4-keto-L-proline to yield 1-(3-(((4methoxyJphenylmethyl]thio)-2-trifluoromethyl-1oxopropyl]-4-oxo-L-proline. c) l-(3-Mercapto-2-trifluoromethyl-l-oxopropyl)-425 oxo-L-proline The product from part (fa) is mixed with trifluoroacetic acid and anisole under nitrogen.

The solvents are removed under vacuum to yield as a residue 1-(3-mercapto-2-trifluoromethyl-l-oxopro30 yl)-4-oxo-L-proline. - 26 Example 16 (S)—X-(3-Mercapto-3-methyl-l-oxopropyl)-4-oxoL-proline a) (S)-1-[3-(Acetylthio)-3-methyl-l-oxopropyl]-45 oxo-L-proline Following the prodecure of Example 1 but substituting D-3-acetylthio-3-methylpropionyl chloride for the 3-acetylthiopropionyl chloride in part (d), one obtains (S)-1-[3-(acetylthio)-310 methyl-l-oxopropyl]-4-oxo-L-proline. b) (S)-1-(3-Mercapto-3-methyl-l-oxopropyl)-4-oxoL-proline The product from part (a) is hydrolyzed with concentrated ammonia to yield (S)-1-(3-mercapto 3-methyl-l-oxopropyl)-4-oxo-L-proline.

Examples 17 - 25 Following the procedure of Example 1 but substituting for the 3-acetylthiopropionyl chloride the acid chloride listed below in 2o Col. I one obtains the acylmercapto product listed below in Col. II. - 27 3-benzoylthiopropionyl chloride 1-[3-(benzoylthio)-1-oxopropyl]- 0 X 1 V β 0» c 0 0 •H 1 •rf 1 1 •H rd ri rd rd rd X 0 1 1 0 0 1 1 X Ci Ci X •ri u 1—1 ·—I r—. CU (0 1 x 0» 0 Sh rd 1 a 0 jj 1 •-4 Λ Sh J X 1—1 X x 4-1 c I r—t 0 rd J jj 0 0 Sh 1 Sh 0 r—ι e X) X r* •er β X r4 1 U 0 X 1 0 0 Sh β CM <0 1 0) (—1 X 1 β •rd I Φ 0 XT 0 rd id *r 0 r—i G 1 Ci >s (0 1 X 0 o Ή pH 1—5 0 a 0 Ρ-Ί Ci Ci •id ι—i Sh t—( «—( 0 rd nJ cu XJ 0 fi Sh X Ci ^i >1 0 1 X u 0) Oi 0 cu Sh Ol X O( •W 0 •H 0 Cl 0 r-4 1 Sh 1 Cf Ci X 0 k >, 0 0 X X CU X 0 44 cu Ό X N 1 | 0 1 1 1 0 •rl 0 QJ c 0 CM X CM rd CM X Ci ( c Φ X '—S Ό * I 0 Sh XT •id X) 0 >—· 1 . CM •—1 >—1 1 Oi 1 rd 1 »—1 rd 0 u—> rd 1 i-i o 1 ’S* 1 1 CM •H 1 1 Ci f*l 1 m rd *—- x cn i“4 Sh a UJ >1 I—> X Φ «—· Sh *—· 0( 1 I ρ—I 1 X t—1 P—5 β 1 X 0 rd Sh rd X 1 r-4 •ri i—J JJ 1 μ 1 I 0< 1 Φ m Sh r-4 1 QJ n Οι o 0 e ι—· 0 0 «—» ε 0 X co id CO 1 1 0 Ci co 1 j X 0 CU ·*· CM H x cu CM rd o 1 l Ci β 1 0 1 o o 1 rd ϋ ‘H •p4 •ri $4 k X 0) X 0( rd 0 rd 0 X Ό X rd Sh >1 rd 1—1 ‘ri r—, s β P-i Λ X rd Ci t-4 X 0 Sh JJ 0 SH 0 Sh JJ XJ C (U β K β a> u 0 0 rd 0 X Q g kJ • Ci Sh X ϋ X l 0 Ol 0 β Ci Ct 0) CM 1 0 rd 0 β rd β Ό rd Ci x id ϋ >1 V •id o V Sh Oi o CU Ρ-» β Ci ••d Ό β rd •fd 0 rd 0 r-4 0 X? •id >1 Ci 0 Sh •ri Sh r-4 X Ci •ri Λ X cu 0 O. Ό X r-i 0 (β X 1 3 σ •rf ϋ >1 rd JJ Φ CM 7 Ud ο o 0 X 1 ε «. •rl I CU > rd N 0 CM 1 CM X CM rd Oi >< c ·—· CM JJ b J c a> rd l_^ 1 CM f—1 •K X ’tf’ 0 Λ >, U-l V r—i rw X •rl 1 β 1 0 kp >, 1 φ <—( CU m 0 m •rf β cn ε 0 I •r| f x 1 Q I 1 1 Ci O Oi Q jj rn X Q CM cn O V r-i q 1 >1 1 •H Qj rH nJ 0 »—1 0 U 5-« >1 M Qj c Qj r—i 0 0 1 X Λ XI c 0 1 0 I flj 0 £ i—f U X Gj 1 0 iH 1 >1 >1 tt x: x; x: 1 jj JJ X» r—> 0 0 0) iH ε ε QJ ε 1 1 c r—f x; *3· 04 •H >5 4J 1 T—| q QJ ·—« 0 QJ 0 «—J 0 μι Λ X I •H Qj Qj 0 m χ: 1 I <—< JJ J rH t r—. 1 *—J I »H rH 0 1 1—> 1 >1 X Ol 0 C 0 ·—' H ω 0 1 I Λ *—* X rH 44 t I QJ l—l f—l •0 0 iH •H •H >1 μι Λ q 0 44 0 rH J—· Λ X3 iH μι 0 >1 d C 0 1—( 0 5h Q q M >5 0 CO C •H 0 QJ Qj x: 0 rH Qj μι iH Qj x: 0) >1 »H jj Ό 42 >1 QJ •H JJ Λ ε Q 0) Ρ •Η O ε QJ >1 iH 1 ε C x: *53* 1 QJ ϋ ·<*> CM x: 1—U 1 Qj rH «—1 >5 | o 44 m •H »—» Q) 1 Λ 1 0 Q 44 CM d - Γ [ (cyclohexyl}carbonyl]thio]- 1-(4-(1 (cyclohexyl)carbonyl] thio]butyroyl chloride 1—oxobutyl]-4—oxo-L-proline - 29 Example 26 (S)-l-(3-Mercapto-2-methyl-l-oxopropyl)-4-oxo-Lproline, 1-adamantanamine salt The product of Example 4 can also be prepared according to the following procedure. a) N-Carbobenzyloxy -4,4-dinethoxy-L-proline. methyl ester A stirred solution of 7.8 g. (0.03 mole) of N-carbobenzyloxy-4-keto-L-proline from Example 1 in 60 ml. of methanol is treated with 96 ml. of trimethyl orthoformate, followed by 0.6 ml. of concentrated sulfuric, acid and allowed to stand overnight at room temperature.

The pale yellow solution is stirred, treated with 1.5 g. of potassium carbonate, followed by 30 ml. of water and the bulk of the solvent is removed on a rotary evaporator to give a syrupy residue which is shaken with 30 ml. of water and 30 ml. of chloroform. After separating the layers the aqueous phase is extracted with additional chloroform (3 x 30 ml.) and the combined organic layers are washed with 45 ml. of saturated sodium chloride solution and dried (MgSO^). Evaporation of the solvent yields 8.4 g. (88%) of N-carbobenzyloxy-4,4-dimethoxy-L-proline, methyl ester. b) N-Carbobenzyloxy-4,4-dimethoxy-L-proline The ester (8.4 g., 0.026 mole) from part (a) is dissolved in 80 ml. of methanol, treated dropwise at -1° to 4° with 18 ml. (0.036 mole) of 2N sodium hydroxide kept at 0° for one hour, and at room temperature overnight. After removing about one half of the solvent on a rotary evaporator, the solution - 30 is diluted with 150 ml. of water, washed with 100 ml. of ether (wash discarded), acidified while cooling with 63 ml. of 1:1 hydrochloric acid to pH 2, and extracted with ethyl acetate (4 x 750 ml.). The combined extracts are washed with 50 ml. of saturated sodium chloride solution, dried (MgSO^), and the solvent evaporated to give 8.0 g. of a pale yellow viscous oil. The oil is dissolved in 35 ml. ethanol, treated with 3.0 g. of cyclohexylamine in 10 ml. of ethanol and diluted to 500 ml. with ether. On seeding and rubbing, the crystalline N-carbobenzyloxy-4,4dimethoxy-L-proline cyclohexylamine salt separated; weight after cooling overnight, 7.0 g., m.p. 157-159° (s, 151) [a]p6 -34° (c, 1% in EtOH). This material is recrystallized from 100 ml. of acetonitrile to give the salt as a colorless solid, m.p. 158-160° (s, 154°) [ajp6 -33° (c, 1% in EtOH).

The N-carbobenzyloxy-4,4-dimethoxy-L-proline cyclohexylamine salt is suspended in 40 ml. of ethyl acetate, stirred and treated with 25 ml. of IN hydrochloric acid. When two clear layers are obtained they are separated, the aqueous phase is extracted with additional ethyl acetate (3 x 40 ml.), the combined organic layers are dried (MgSO^), and the solvent evaporated, finally at 0.2 mm and 40° to yield 7.2 g. (70%) of N-carbobenzyloxy-4,4-dimethoxyL-proline as a pale yellow viscous syrup. c) 4,4-Dimethoxy-L-proline A solution of N-carbobenzyloxy-4,4-dimethoxy30 L-proline (72 g., 0.022 mole) in 210 ml. of methanol50531 - 31 water (2:1) is treated with 2.3 g. of 5% palladiumcarbon and shaken on a Parr hydrogenator for 6 hours. The catalyst is filtered off under nitrogen, washed with methanol, and the combined filtrates are evaporated, finally at 0.1 - 0.2 mm., to give a partly crystalline residue. This residue is taken up in 200 ml. of methanol and the evaporation repeated. When the solid is rubbed under ether (evaporation again repeated) there is obtained 3.6 g. (95%) of nearly colorless 4,4-dimethoxy-L-proline, m.p. 192-194° (dec.); [a]£6 -47° (c, 1% in MeOH).

A sample crystallized from methanol-ether is colorless and melts at 197-198° (dec.); [aj^6 ”49° (c, 1% in MeOH). d) (S)-1-[3-(Acetylthio)-2-methyl-l-oxopropyl]-4,4dimethoxy-L-proline A stirred solution of 3.3 g. (0.019 mole) of 4,4-dimethoxy-L-proline in 50 ml. of water is cooled to 5° and brought to pH 8.5 by the addition of 25% sodium carbonate solution (w/v). Then while continuing stirring and cooling, a solution of 3.8 g. (0.021 mole) of D-3-acetylthio-2-methylpropanoyl chloride in 5 ml. of ether is added portionwise while maintaining the pH at 7.5 - 8.5 by dropwise addition of 25% sodium carbonate solution. When the pH has stabilized at 8.2 - 8.4 (after about 15 minutes), stirring and cooling is continued for a total of one hour. The solution is then washed with 50 ml. of ethyl acetate (wash discarded),layered over with 50 ml. of ethyl acetate, cooled, stirred, acidified carefully with 1:1 hydro50531 - 32 chloric acid to pH 2.0, saturated with sodium chloride, and the layers separated. The aqueous phase is extracted with additional ethyl acetate (3 x 50 ml.), the combined organic layers are dried (MgSO^), and the solvent evaporated, finally at 0.2 mm, to give 6.7 g. of syrupy product. This syrup is treated in 70 ml. of ethyl acetate with 3.9 g. of dicyclohexylamine to give 6.5 g. of colorless (S)-l[3- (acetylthio) -2-methyl-l- oxopropyl 1-4,4dimethoxy-L-proline dicyclohexylamine salt in two crops (3,1 g. and 3.4 g.), m.p. 158-160° (s, 145°). [α)θ6 -71° (c, 1% in EtOH).

Following recrystallization from 20 ml. of hot ethyl acetate-60 ml. of hexane, the colorless solid salt weighs 6.0 g., m.p. 158-166° (s, 155°), [ex] -69° (c, 1% in EtOH).

The dicyclohexylamine salt is converted to the free acid by suspending 5.0 g. in 50 ml. of ethyl acetate, cooling and treating with 60 ml. of 10% potassium bisulfate solution to give 2 clear layers. After separating, the aqueous phase is extracted with ethyl acetate (3 x 50 ml.), the combined organic layers are dried (MgSO^), and the solvent evaporated, finally at 0.1-0.2 mm. and 45° to give 4.1 g. (69%) of (S)-1-[3-(acetylthio)-2-methyl-l-oxopropyl]4,4-dimethoxy-L-proline as a viscous, almost glasslike material -112° (c, 1% in EtOH). e) (S) —1—(3-Mercapto-2-methyl-l-oxopropyl)-4,4dimethoxy-L-proline Argon is passed through a cold solution of 8.5 ml. of concentrated ammonium hydroxide in 20 ml. of ~ 33 water for 0.25 hour. The latter is then added while cooling and under a blanket of argon to 4.1 g. (0.013 mole) of (S)-l~[3-(acetylthio)-2-methyl-loxopropyl]-4,4-dimethoxy-L-proline and the mixture is swirled in an icebath until a pale yellow solution is obtained (about 15 minutes). Stirring under argon is continued at room temperature for an additional 2 hours, then the solution is extracted with 30 ml. of ethyl acetate (this and subsequent operations are carried out as much as possible under an argon atmosphere). The aqueous layer is cooled, stirred, layered over with 30 ml. of ethyl acetate, and acidified portionwise with approximately 16 ml. of 1:1 hydrochloric acid. The layers are separated, the aqueous phase is extracted with additional ethyl acetate (3 x 30 ml.), the combined ethyl acetate layers are dried (MgSO^), and the solvent evaporated to give 3.5 g. (100%) of (S)-l-(3-mercapto-2-methyl-l-oxopropyl)4,4-dimethoxy-L-proline as a colorless, viscous syrup, -72° (c, 1% in EtOH).

The latter (3.4 g.) is triturated with 20 ml. of ethyl acetate, rubbed, diluted with 30 ml. of hexane, and cooled to give a colorless solid, weight 2.6 g., m.p. 108-110°, [a]^ -77° (c, 1% in EtOH). §0531 - 34 f) (S)-1-(3-Mercapto-2-methyl-l-oxopropyl)-4oxo-L-proline, 1-adamantanamine salt The (S)-1- (3-mercapto-2-methyl-l-oxopropyl)-4,4dimethoxy-L-proline (0.4 g., 0.0014 mole) is added 5 to 8 ml. of stirred N HCl through which argon had previously been passed for ten minutes. After a solution is obtained, the flask is stoppered under argon and kept overnight at room temperature.

The following steps are also carried out under an atmosphere of argon to the extent possible. To the stirred solution there is added 20 ml. of methylene chloride and after saturating with sodium chloride the layers are separated. The aqueous phase is extracted with additional methylene chloride (3 x 15 ml.), the organic layers are combined, dried (MgSO^), and the solvents evaporated to give a foamy residue. Following trituration with ether (evaporation repeated), 0.26 g. of (S)-1-(3-mercapto-2-methyl-l-oxopropyl)-4-oxo-Lproline are obtained as an amorphous solid (hygroscopic). 0.245 g. of this product is dissolved in 7 ml. of acetonitrile and treated with 0.17 g. of 1-adamantanamine to precipitate the salt as a voluminous solid.

After cooling overnight, the colorless material is filtered, washed with cold acetonitrile and ether, and dried in vacuo to yield 0.36 g. of (S)-l-(3-mercapto2-methyl-1-oxopropyl)-4-oxo-L-proline, 1-adamantanamine salt (1:1); m.p. 188-190° (dec.); s. 184°; [a]^5 -13° (c, 1% in methanol).

Anal. Calc'd. for CgH^NC^S · C H^N · 0,25 H2<0: C, 58.96; H, 8.02; N, 7.24; S, 8.29 C, 58.60; H, 8.37; N, 7.16; S, 8.29.

Found: 50S31 - 35 Example 27 1,1'-[Dithiobis(l-oxo-3,l-propanediyl)3 bis[4-oxo-Lproline] The product from Example 2 is dissolved in water and the pH adjusted to 6.5 by the addition of IN sodium hydroxide. To this stirred solution is added dropwise a 0.5 M iodine solution in 95% ethanol (6.34 g. iodine/50 ml. solution) while maintaining the pH at 5.5 to 6.5 with IN sodium hydroxide. Excess iodine is removed with dilute sodium thiosulfate and the solution is concentrated, cooled and acidified with 1:1 hydrochloric acid. Solvent is added and the layers are separated. The organic layer is dried MgSO^) and the solvent evaporated to yield as a residue 1,1'-[dithiobis(l-oxo-3,1-propanediyl)3 bis(4-oxo-Lproline] .

Example 28 1-[3-(Acetylthio)-1-oxopropyl]-4-oxo-L-proline, methyl ester A solution of the product of Example 1 in ether is treated with a slight excess of diazomethane. After standing at room temperature for two hours, the solvent is evaporated to give 1-[3-(acetylthio)-loxopropyl]-4-oxo-L-proline, methyl ester.

Example 29 1—(3—Mercapto—1—oxopropyl)—4—oxo—L—proline, methyl ester The product from Example 28 j.s hydrolyzed with concentrated ammonia according to the procedure of Example 2 to yield l-(3-mercapto-l-oxopropyl)-4-oxo50531 - 36 L-proline, methyl ester.

Example 30 1- (3-Mercapto-l-oxopropyl)-4-oxo-L-proline, sodium salt A solution of 1.0 g. of the product of Example 2 is dissolved in 10 ml. of water and treated with one equivalent of sodium bicarbonate.

The solution is freeze-dried to give 1-(3-mercapto-loxopropyl)-4-oxo-L-proline, sodium salt. in a similar manner, by employing potassium bicarbonate the corresponding potassium salt is obtained.

Example 31 (S)-1-(3-Mercapto-2-methyl-l-oxopropyl)-4-oxo-L15 proline, sodium salt A solution of 1.0 g. of the product of Example 4 is dissolved in 10 ml. of water and treated with one equivalent of sodium bicarbonate. The solution is freeze-dried to give 1-(3-mercapto-220 methyl-l-oxopropyl)-4-oxo-L-proline, sodium salt.

In a similar manner, by employing potassium bicarbonate the corresponding potassium salt is obtained.

Example 32 1000 tablets each containing the following ingredients: 1-(3-mercapto-l-oxopropyl)4-oxo-L-proline 100 mg.

Corn starch 50 mg.

Gelatin 7.5 mg. - 37 Avicel (Trade Mark) microcrystalline cellulose) 25 mg Magnesium stearate 2.5 mg. 185 mg. are prepared (from sufficient bulk quantities) by mixing the 1-(3-mercapto-l-oxopropyl)-4-oxo-L-proline and corn starch with an aqueous solution of the gelatin. The mixture is dried and ground to a fine powder. The Avicel and then the magnesium stearate are admixed with granulation. This mixture is then compressed in a tablet press to form 1000 tablets each containing 100 mg. of active ingredient.

Example 33 Tablets each containing 100 mg. of (S)-l(3-mercapto-2-methyl-l-oxopropyl)-4-oxo-L-proline are produced as described in Example 32.

Example 34 1000 tablets each containing 50 mg. of 1-(3mercapto-l-oxopropyl)-4-oxo-L-proline are produced from the following ingredients: 1-(3-mercapto-l-oxopropyl)25 4-oxo-L-proline 50 g. Lactose 100 g· Avicel 150 g- Corn starch 50 g· Magnesium stearate 5 g.

The 1-(3-mercapto-l-oxopropyl)-4-oxo-L-proline, lactose, and Avicel are admixed, and then blended with corn starch. Magnesium stearate is added. The dry mixture is compressed in a tablet press to - 38 form 1000 355 mg. tablets each containing 50 mg. of active ingredient. The tablets are coated with a solution of Methocel (Trade Mark) E 15 (methyl cellulose) including as a color a lake containing yellow #5.

Example 35 Tablets each containing 50 mg. of (S)-l(3-mercapto-2-methyl-l-oxopropyl)-4-oxo-L-proline are prepared as described in example 34.

Example 36 Two piece #1 gelatin capsules each containing 100 mg. of 1-(3-mercapto-l-oxopropyl)-4-oxo-Lproline, sodium salt, are filled with a mixture of the following ingredients: 1-(3-mercapto-l-oxopropyl)- 4-oxo-L-proline 100 mg. Magnesium stearate 7 mg. Lactose 193 mg. Example 37 Gelatin capsules containing 100 mg. of (S)-l- (3-mercapto-2-methyl-l-oxopropyl)-4-oxo-L-proline, sodium salt are prepared as described in Example -36.

Example 38 An injectable solution is produced as follows: 1- (3-mercapto-l-oxopropyl)-4oxo-L-proline 500 g. Methyl paraben 5 g. Propyl paraben 1 g- Sodium chloride 25 g· Water for injection qs. 5 1 - 39 The active substance, preservatives and sodium chloride are dissolved in 3 liters of water and then the volume is brought up to 5 liters. The solution is filtered through a sterile filter and aseptically filled into presterilized vials which are then closed with presterilized rubber closures. Each vial contains 5 ml. of solution in a concentration of 100 mg. of active ingredient per ml. of solution for injection.

Example 39 An injection solution containing (5)-1-(3mercapto-2-methyl-l-oxopropyl)-4-oxo-L-proline is prepared as described in Example 38.

Example 40 6000 tablets each containing the following ingredients: 1-(3-mercapto-l-oxopropyl)- 4-oxo-L-proline 100 mg. Avicel (microcrystalline cellulose) 100 mg. Hydrochlorothiazide 12.5 mg. Lactose U.S.P. 113 mg. Corn starch U.S.P 17.5 mg. Stearic acid U.S.P. 7 mg. 350 mg. are produced from sufficient bulk quantities by slugging the 1-(3-mercapto-l-oxopropyl)-4-oxo-Lproline, Avicel, and a portion of the stearic acid.

The slugs are ground and passed through a #2 screen, then mixed with the hydrochlorothiazide, lactose, corn S0531 - 40 starch and remainder of the stearic acid. The mixture is compressed into 350 mg. capsule shaped tablets in a tablet press. The tablets are scored for dividing in half.

Example 41 Tablets each containing (S)-l-(3-mercapto-2methyl-l-oxopropyl)-4-oxo-L-proline and hydrochlorothiazide can be prepared as described in Example 40.

The product of Examples 1,3,5 to 25, and 27 to 29 can be formulated according to the procedures of Examples 32-41.

Claims (1)

1. A compound of the formula II c X \ R, R, 0 (H.C) (CH.) | 3 I 1 ll V I q 5 R.-S-(CH)- C- C — N - C-COOR 4 m or a physiologically acceptable salt thereof, wherein R is hydrogen or lower alkyl; 10 R^ and Rj are independently selected from the group consisting of hydrogen, lower alkyl, lower alkylthio, -(CH 2 ) n ~SH, and halo substituted lower alkyl; Rj is hydrogen or lower alkyl provided that 15 r 2 is lower alkyl only when R^ is lower alkyl; m is zero, one or two; n is one, two or three; p and q are each one or two provided that both are not two; and 2. Q R^ is hydrogen, a hydrolyzably removable protecting group, a chemically removable protecting group, or provided that neither R^ nor R^ is -(CH 2 ) n -SH - 42 R, R. 0 (H,C) ,3 13 ι, 2, p 0 1' c \ ^q -S- (CH) — C— C- N - C-COOR m . , 2. The compound of Claim 1 wherein p is two and q is one. 3. The compound of Claim 1 wherein p is one and q is two. 4. The compound of Claim 1 wherein p and q are both one. 5. A compound of the formula · R.-S-(CH,)—C- C -N. 4 2 m COOH (L) or a physiologically acceptable salt thereof, wherein R^ and R 2 are both lower alkyl of 1 to 4 carbons or R 2 is hydrogen and R^ is hydrogen, lower alkyl of 1 to 4 carbons, trifluoromethyl, methylthio, - 43 or mercaptomethyl; m is 2ero or one; and R^ is hydrogen, lower alkanoyl of 1 to 4 carbons, or benzoyl. 6. The compound of Claim 5 wherein R^ and R 2 are both methyl and n is one. 7. The compound of Claim 5 wherein R 2 is hydrogen; R^ is trifluoromethyl; and m is one. 8. The compound of Claim 5 wherein R 2 is hydrogen; R^ is methylthio; and m is one. 9. The compound -of Claim 5 wherein R 2 is hydrogen; R^ is mercaptomethyl; and m is one. 10. The compound of Claim 5 wherein R^ and R 2 are both hydrogen. 11. The compound of Claim 10 wherein m is one. 12. The compound of Claim 11 wherein R^ is acetyl. 13. The compound of Claim 11 wherein R^ is hydrogen. 14. The compound of Claim 10 wherein m is zero. 15. The compound of Claim 5 wherein R 2 is hydrogen, Rj is methyl, and m is one. 16. The compound of Claim 15 wherein R^ is acetyl. 17. The compound of Claim 15, wherein R^ is hydrogen. 18. The compound of Claim 17, (S)-l-(3-mercapto2-methyl-l-oxopropyl)-4-oxo-L-proline. - 4β 19. A composition for treating hypertension comprising a pharmaceutically acceptable carrier and one or more hypotensive agents or physiologically acceptable salts thereof of the formula H c z \ I 3 Γ 1 J '|“ 2 ' 5 R.-S-(CH)— C— C- N - C-COOR 4 Rl ι wherein R is hydrogen or lower alkyl; 10 Rj and are independently selected from .the group consisting of hydrogen, lower alkyl, lower alkylthio, -(CH.) -SH, and halo substituted lower i n alkyl; R^ is hydrogen or lower alkyl provided that 15 R^ is lower alkyl only when R^ is lower alkyl; m is zero, one, or two; n is one, two, or three; p and q are each one or two provided that both are not two; 2 0 R^ is hydrogen, a hydrolyzably removable protecting group, or provided that neither R^ nor R, is -(CH.) -SH 3 2. n I -S-(CH)· C-COOR ι H 20. The composition hypotensive agent is of the of Claim 19 wherein the formula *1 ° R.-S-(CH„)„—C —C4 x in COOH (L) 10 wherein R^ and Rj are both lower alkyl of 1 to 4 carbons or Rj is hydrogen and R^ is hydrogen, lower alkyl of 1 to 4 carbons, trifluoromethyl, methylthio, or mercaptomethyl; m is zero or one; and 15 R^ is hydrogen, lower alkanoyl of 1 to 4 carbons, or benzoyl. 21. The composition of Claim 20 wherein R^, Rj, and R^ are hydrogen and m is one. §Θ531 - 46 22. : The composition of Claim 20 wherein R 1 is methyl; R, and R^ are hydrogen; and m is one. 23. The composition of Claim 19 also including a diuretic. 24. A process for preparing a keto-substituted proline or pipecolic acid of the formula ll c X \ R, R. 0 (H,C) n (CH,) I 3 I 1 II I p I 2q R.-S-(CH) C C — N - C-COOR 4 m or a physiologically acceptable salt thereof, wherein R is hydrogen or lower alkyl; R^ and are independently selected from the 15 group consisting of hydrogen, lower alkyl, lower alkylthio, -(CH 2 ) n -SH, and halo substituted lower alkyl; R 2 is hydrogen or lower alkyl provided that R 2 is lower alkyl only when is lower alkyl; 2o m is zero, one or two; n is one, two or three; p and q are each one or two provided that both are not two; and R 4 is hydrogen, a hydrolyzably removable protect25 ing group, a chemically removable protecting group, or provided that neither R 1 nor R^ is -iCH 2 ) n ~SH 47 Ο II C\ ι 3 -£-(CH) R. 0 (H.C) 11 ii 2 , P C — C-C-C00R r 2 h characterized by (a) coupling a keto-substituted proline or pipecolic acid of the formula COOR with an acid or a reactive derivative thereof of the formula R, R-l R J-SI 3 (CH). -COOH wherein RJ is hydrogen or a hydrolyzably or chemically 15 removable protecting group to form a product wherein R^ is hydrogen or a hydrolyzably or chemically removable protecting group; and if desired, removing said protecting group and wherein R^ and R^ are other than -(CH 2 ) n ~SH, oxidizing said product wherein R^ is 20 hydrogen with iodine to form a product wherein R 4 is -S-(CH) · in R. 0 (H,C) I II I p C-C-N— < c ? 2 ’ g ϊ-COOR or (b) deprotecting the desired product wherein the keto function is protected. t ,. 50531 I 48 5 25. The process of Claim24 wherein p is two and q is one. 26. The process of Claim 24 wherein p is one and q is two. 27. The process of Claim 24 Wherein p and q are both one. 25. 28. A process according to claim 24 wherein the product is of the formula C - N. COOH V S - (CH 2>m C ~ R 2 or a physiologically acceptable salt thereof, wherein and R 2 are both lower alkyl of 1 to 4 carbons or R 2 is hydrogen and R^ is hydrogen, lower alkyl of 1 to 4 carbons, trifluoromethyl, methylthio, or mercaptomethyl; m is zero or one; and R^ is hydrogen, lower alkanoyl of 1 to 4 carbons, or benzoyl. (L) - 49 26. 29. The process of Claim28 wherein R^ and Rj are both methyl and m is one. 27. 30. The process of Claim 28 wherein Rj is hydrogen; E^ is trifluoromethyl; and m is one. 28. 31. The process of Claim 2S wherein Rj is hydrogen; R^ is methylthio; and m is one. 29. 32. The process of Claim 28 wherein Rj is hydrogen; R^ is mercaptomethyl; and m is one. 33, The process R. are both hydrogen. of Claim 28 wherein R^ and 34. The process of Claim 33 wherein m is ( 35. The process of Claim 34 wherein R, is 4 acetyl. 36. The process of Claim 3 4 wherein R^ is hydrogen. 37. The process of Claim 33 wherein m is zero. 38. The process of Claim 28 wherein Rj is hydrogen, R is methyl, and m is one. 39. The process of Claim38 wherein R^ is acetyl. 40. The process of Claim 38 wherein R^ is hydrogen. 41. The process of Claim 4o wherein the product is (S)—1—(3-mercapto-2—methyl—1—oxonropyl)— 4-oxo-L-proline. 5U531 - 50 42. A process for preparing a compound as claimed in claim 1, substantially as herein described with reference to any of the Examples. 43 A compound according to Claim 1 when obtained by 5 a procees according to any of claims 24 to 42 , 44. A composition according to claim 19, substantially as herein described.