IE45013L - Purifying insulin - Google Patents
Purifying insulinInfo
- Publication number
- IE45013L IE45013L IE770044A IE4477A IE45013L IE 45013 L IE45013 L IE 45013L IE 770044 A IE770044 A IE 770044A IE 4477 A IE4477 A IE 4477A IE 45013 L IE45013 L IE 45013L
- Authority
- IE
- Ireland
- Prior art keywords
- insulin
- extract
- substances
- removal
- ion exchange
- Prior art date
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title abstract 22
- 102000004877 Insulin Human genes 0.000 title abstract 11
- 108090001061 Insulin Proteins 0.000 title abstract 11
- 229940125396 insulin Drugs 0.000 title abstract 11
- 239000000126 substance Substances 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 3
- 238000001816 cooling Methods 0.000 abstract 2
- 238000005342 ion exchange Methods 0.000 abstract 2
- 238000011084 recovery Methods 0.000 abstract 2
- 150000001408 amides Chemical class 0.000 abstract 1
- 238000004040 coloring Methods 0.000 abstract 1
- 238000001502 gel electrophoresis Methods 0.000 abstract 1
- 210000004907 gland Anatomy 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 229910021645 metal ion Inorganic materials 0.000 abstract 1
- 210000000496 pancreas Anatomy 0.000 abstract 1
- 229920002401 polyacrylamide Polymers 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 238000001223 reverse osmosis Methods 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C07K14/625—Extraction from natural sources
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Endocrinology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Diabetes (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention relates to monocomponent insulin purified to such a degree that it shows only a single component when analyzed by amide gel electrophoresis using a gel containing 15% polyacrylamide. It, furthermore, relates to a method of preparing such insulin from an insulin containing raw extract prepared from pancreas glands, consisting in the working up of the extract in such a manner that the insulin is maintained in dissolved state during the whole processing, without change of phase, until the final recovery of the insulin, the undesired substances being from the beginning of the process until the end removed from the different solvents used. A preferred method comprises removal of fat from the extract by cooling to a temperature below -25.degree.C and separation of the crystallized fat, concentration of the extract and simultaneous removal of impurities present in the extract, both substances having a larger molecule than insulin and substances having a smaller molecule than insulin, by means of reverse osmosis plant for removal of colouring substances and salts, further purification by means of ion exchange and recovery of the insulin from the concentrated purified solution resulting from the ion exchange treatment by precipitation with metal ions under strong cooling. The monocomponent insulin may be used in insulin preparations of any kind for clinical use.
[CA1112639A]
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1740/76A GB1581824A (en) | 1976-01-16 | 1976-01-16 | Preparation of insulin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE45013L true IE45013L (en) | 1977-07-16 |
| IE45013B1 IE45013B1 (en) | 1982-06-02 |
Family
ID=9727172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE44/77A IE45013B1 (en) | 1976-01-16 | 1977-01-11 | Improvements in or relating to the preparation of insulin |
Country Status (15)
| Country | Link |
|---|---|
| AT (2) | AT364717B (en) |
| AU (1) | AU514888B2 (en) |
| BE (1) | BE850387A (en) |
| CA (1) | CA1112639A (en) |
| DE (1) | DE2701092A1 (en) |
| DK (1) | DK14177A (en) |
| EG (1) | EG13113A (en) |
| ES (1) | ES455065A1 (en) |
| FI (1) | FI64508C (en) |
| FR (1) | FR2338250A1 (en) |
| GB (1) | GB1581824A (en) |
| IE (1) | IE45013B1 (en) |
| NL (1) | NL7700455A (en) |
| NO (2) | NO149875C (en) |
| SE (1) | SE445972B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK311477A (en) * | 1977-07-08 | 1979-01-09 | Leo Lab | PROCEDURE FOR PREPARING PURE INSULIN |
| US4459226A (en) * | 1982-02-26 | 1984-07-10 | Eli Lilly And Company | Process for recovering insulin |
| BG65045B1 (en) * | 2001-12-03 | 2007-01-31 | "Софарма" Ад | Method for the production of highly purified monocomponent insulin |
| EP2670773A1 (en) | 2011-02-01 | 2013-12-11 | Novo Nordisk A/S | Purification of insulin |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1966573C3 (en) * | 1969-08-07 | 1979-08-30 | Novo Terapeutisk Laboratorium A/S, Kopenhagen | Method for purifying insulin |
| GB1503919A (en) * | 1974-07-19 | 1978-03-15 | Leo Sa Lab | Recovery of insulin |
-
1976
- 1976-01-16 GB GB1740/76A patent/GB1581824A/en not_active Expired
-
1977
- 1977-01-10 AT AT0007977A patent/AT364717B/en not_active IP Right Cessation
- 1977-01-11 IE IE44/77A patent/IE45013B1/en unknown
- 1977-01-12 DE DE19772701092 patent/DE2701092A1/en not_active Withdrawn
- 1977-01-13 SE SE7700322A patent/SE445972B/en unknown
- 1977-01-13 AU AU21317/77A patent/AU514888B2/en not_active Expired
- 1977-01-14 FR FR7701077A patent/FR2338250A1/en active Granted
- 1977-01-14 FI FI770112A patent/FI64508C/en not_active IP Right Cessation
- 1977-01-14 NO NO770119A patent/NO149875C/en unknown
- 1977-01-14 CA CA269,730A patent/CA1112639A/en not_active Expired
- 1977-01-14 ES ES455065A patent/ES455065A1/en not_active Expired
- 1977-01-14 BE BE174084A patent/BE850387A/en not_active IP Right Cessation
- 1977-01-14 DK DK14177A patent/DK14177A/en not_active Application Discontinuation
- 1977-01-15 EG EG30/77A patent/EG13113A/en active
- 1977-01-17 NL NL7700455A patent/NL7700455A/en not_active Application Discontinuation
-
1980
- 1980-11-06 AT AT0546880A patent/AT366578B/en not_active IP Right Cessation
-
1982
- 1982-09-03 NO NO822985A patent/NO822985L/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AT364717B (en) | 1981-11-10 |
| IE45013B1 (en) | 1982-06-02 |
| FR2338250A1 (en) | 1977-08-12 |
| DK14177A (en) | 1977-07-17 |
| FI64508C (en) | 1983-12-12 |
| AT366578B (en) | 1982-04-26 |
| NO149875B (en) | 1984-04-02 |
| NO149875C (en) | 1984-07-11 |
| NO822985L (en) | 1977-07-19 |
| CA1112639A (en) | 1981-11-17 |
| BE850387A (en) | 1977-05-02 |
| NO770119L (en) | 1977-07-19 |
| FR2338250B1 (en) | 1980-04-04 |
| ATA7977A (en) | 1981-04-15 |
| SE7700322L (en) | 1977-07-17 |
| GB1581824A (en) | 1980-12-31 |
| FI64508B (en) | 1983-08-31 |
| FI770112A7 (en) | 1977-07-17 |
| AU514888B2 (en) | 1981-03-05 |
| ATA546880A (en) | 1981-09-15 |
| ES455065A1 (en) | 1978-05-01 |
| DE2701092A1 (en) | 1977-07-28 |
| EG13113A (en) | 1983-03-31 |
| NL7700455A (en) | 1977-07-19 |
| AU2131777A (en) | 1978-07-20 |
| SE445972B (en) | 1986-08-04 |
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