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HUP9901725A2 - Szubtilizinváltozatok - Google Patents

Szubtilizinváltozatok

Info

Publication number
HUP9901725A2
HUP9901725A2 HU9901725A HUP9901725A HUP9901725A2 HU P9901725 A2 HUP9901725 A2 HU P9901725A2 HU 9901725 A HU9901725 A HU 9901725A HU P9901725 A HUP9901725 A HU P9901725A HU P9901725 A2 HUP9901725 A2 HU P9901725A2
Authority
HU
Hungary
Prior art keywords
original
amino acids
hydrophobic domain
sübtilase
glycine
Prior art date
Application number
HU9901725A
Other languages
English (en)
Inventor
Peter Bauditz
Jan Klugkist
Peter Markvardsen
Laurens Nicolaas Sierkstra
Claus Von Der Osten
Original Assignee
Unilever N.V.,
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever N.V., filed Critical Unilever N.V.,
Publication of HUP9901725A2 publication Critical patent/HUP9901725A2/hu
Publication of HUP9901725A3 publication Critical patent/HUP9901725A3/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A találmány tárgyát detergenskészítmények képezik, amelyek őlyanszűbtilázváltőzatőt tartalmaznak, amelyben az eredeti szűbtilázhibrőfób dőménjében vagy annak közvetlen közelében elhelyezkedő egyvagy több aminősav az eredetinél nagyőbb hidrőfőbitású aminősavval vanhelyettesítve, mely hidrőfób dőmén a (BASBPN-számőzás szerint) 129. és131. helyen prőlint, 165. helyen izőleűcint, 167. és 171. helyen pedigtirőzint tartalmaz, a hidrőfób dőmén közvetlen közelében lévőaminősavak közé pdig a (BASBPN-számőzás szerint) 136. helyenglűtaminsav, 159. helyen glicin, 164. helyen szerint 170. helyenarginin, 194. helyen alanin és 195. helyen glicin tartőzik, ide nemértve a BAPB92-szűbtilizin R170M, R170I és R170I váltőzatait. Atalálmány szerinti enzimváltőzatők detergenskészítményekben, különösenfőlyékőny detergensekben és szappanrűdakban jőbb stabilitást és/vagymősóhatást műtatnak, mint az eredeti, vad típűsú enzimek. A találmányszerinti enzimváltőzatők előnyösen alkalmazhatók főlyékőnydetergenskészítményekben és szappanrűdakban. ŕ
HU9901725A 1995-05-05 1996-04-12 Subtilisin variants HUP9901725A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP95201161 1995-05-05

Publications (2)

Publication Number Publication Date
HUP9901725A2 true HUP9901725A2 (hu) 1999-09-28
HUP9901725A3 HUP9901725A3 (en) 2001-09-28

Family

ID=8220259

Family Applications (1)

Application Number Title Priority Date Filing Date
HU9901725A HUP9901725A3 (en) 1995-05-05 1996-04-12 Subtilisin variants

Country Status (12)

Country Link
EP (1) EP0827531A2 (hu)
JP (1) JPH11505275A (hu)
AR (1) AR001863A1 (hu)
AU (1) AU5646596A (hu)
BR (1) BR9608126A (hu)
CA (1) CA2217162A1 (hu)
CZ (1) CZ349797A3 (hu)
HU (1) HUP9901725A3 (hu)
PL (1) PL184399B1 (hu)
SK (1) SK284609B6 (hu)
WO (1) WO1996034935A2 (hu)
ZA (1) ZA963567B (hu)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9621436D0 (en) * 1996-10-15 1996-12-04 Unilever Plc Enzymatic compositions
BR9712473B1 (pt) * 1996-11-04 2009-08-11 variantes de subtilase e composições.
CN1272137A (zh) 1997-08-29 2000-11-01 诺沃挪第克公司 蛋白酶变体及组合物
KR100762164B1 (ko) * 1997-11-21 2007-10-01 노보자임스 에이/에스 프로테아제 변이체 및 조성물
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
KR100660799B1 (ko) * 1998-12-18 2006-12-26 노보자임스 에이/에스 활성부위 루프 영역에 추가적 아미노산 잔기를 가지는서브그룹 ｉ-ｓ１과 ｉ-ｓ２의 서브틸라제 효소
AU773009B2 (en) * 1998-12-18 2004-05-13 Novozymes A/S Subtilase enzymes of the I-SI and I-S2 sub-groups having an additional amino acid residue in an active site loop region
JP4768128B2 (ja) * 1998-12-18 2011-09-07 ノボザイムス アクティーゼルスカブ 活性部位ループ領域中に追加のアミノ酸残基を有するサブチラーゼ酵素サブグループｉ−ｓ１及びｉ−ｓ２
WO2000037622A1 (en) * 1998-12-18 2000-06-29 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having an additional amino acid residue in an active site loop region
CN1333826A (zh) * 1998-12-18 2002-01-30 诺沃奇梅兹有限公司 在活性位点环区中具有额外氨基酸残基的i－s1和i－s2亚组枯草杆菌酶
ATE407200T1 (de) * 1999-05-20 2008-09-15 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurenrest zwischen position 131 und 132
MXPA01011836A (es) * 1999-05-20 2002-06-21 Novozymes As Enzimas subtilasas de los sub-grupos i-s1 e i-s2 que tienen al menos un residuo de un aminoacido adicional entre las posiciones 97 y 98.
EP1183343B2 (en) * 1999-05-20 2013-11-27 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 125 and 126
WO2000071687A1 (en) * 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 129 and 130
WO2000071688A1 (en) * 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 126 and 127
EP1183337B1 (en) * 1999-05-20 2008-09-17 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 132 and 133
EP1183342B2 (en) * 1999-05-20 2012-06-13 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129
AU4392300A (en) * 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 130 and 131
AU4392900A (en) * 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 127 and 128
CN1415011B (zh) * 1999-12-15 2010-12-08 诺沃奇梅兹有限公司 对蛋渍具有改进洗涤性能的枯草杆菌酶变体
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
US7888093B2 (en) 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US20080221008A1 (en) * 2006-10-06 2008-09-11 Novozymes A/S Detergent compositions and the use of enzyme combinations therein
US20100192985A1 (en) 2008-11-11 2010-08-05 Wolfgang Aehle Compositions and methods comprising serine protease variants
BR122013014157A2 (pt) * 2008-11-11 2018-11-13 Danisco Us Inc. composições de limpeza compreendendo variantes de subtilisina de bacillus, bem como processo de limpeza
CA3257053A1 (en) 2022-05-14 2023-11-23 Novozymes A/S COMPOSITIONS AND METHODS FOR THE PREVENTION, TREATMENT, SUPPRESSION AND/OR ELIMINATION OF PHYTOPATHOGENIC INFESTATIONS AND INFECTIONS

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR9006827A (pt) * 1989-06-26 1991-08-06 Unilever Nv Composicoes detergentes enzimaticas
DE69212390T2 (de) * 1991-05-01 1997-01-09 Unilever Nv Stabilisierte Enzyme enthaltende Waschmittelzusammensetzungen
EP0995801A1 (de) * 1991-07-27 2000-04-26 Genencor International GmbH Verfahren zur Verbesserung des Stabilität von Enzymen und stabilisierte Enzyme
DE4231726A1 (de) * 1992-09-23 1994-03-24 Cognis Bio Umwelt Mutierte subtilisinartige Serinproteasen
DK39093D0 (da) * 1993-04-01 1993-04-01 Novo Nordisk As Enzym
MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine

Also Published As

Publication number Publication date
SK146797A3 (en) 1998-03-04
CZ349797A3 (cs) 1998-04-15
BR9608126A (pt) 1999-02-09
SK284609B6 (sk) 2005-07-01
EP0827531A2 (en) 1998-03-11
PL323188A1 (en) 1998-03-16
CA2217162A1 (en) 1996-11-07
PL184399B1 (pl) 2002-10-31
WO1996034935A3 (en) 1997-01-16
AU5646596A (en) 1996-11-21
JPH11505275A (ja) 1999-05-18
HUP9901725A3 (en) 2001-09-28
AR001863A1 (es) 1997-12-10
WO1996034935A2 (en) 1996-11-07
ZA963567B (en) 1997-11-06

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Legal Events

Date Code Title Description
FD9A Lapse of provisional protection due to non-payment of fees