HK1233249B

HK1233249B - Bace1 inhibitors

Info

Publication number: HK1233249B
Application number: HK17106753.5A
Authority: HK
Inventors: 比约恩．巴特尔斯; 菲利普．奎尼; 科西莫．多伦特; 沃尔夫冈．古帕; 沃尔夫冈．哈普; 安德烈亚斯．库格斯塔特; 乌尔丽克．奥布斯特．森德; 延斯-乌韦．彼得斯; 马克．罗杰斯-埃文斯; 沃尔特．维凡; 托马斯．沃尔特林
Original assignee: 豪夫迈．罗氏有限公司
Priority date: 2014-10-10
Filing date: 2015-10-07
Publication date: 2020-02-28

Description

BACE1 inhibitors

背景技术Background Art

阿尔茨海默病(AD)是中枢神经系统的神经变性疾病并且导致中年以上人口中的进行性痴呆的产生。其临床症状是记忆、认知、暂时和局部定向、判断和推理的损害，以及严重的情绪紊乱。目前还没有可以预防该疾病或其进展或稳定地逆转其临床症状的有效治疗。在所有具有高预期寿命的社会中AD成为主要的健康问题，并且也成为这些社会健康体系的显著经济负担。Alzheimer's disease (AD) is a neurodegenerative disease of the central nervous system and leads to the development of progressive dementia in the middle-aged and older population. Its clinical symptoms are impairment of memory, cognition, temporal and local orientation, judgment and reasoning, as well as severe mood disturbances. There is currently no effective treatment that can prevent the disease or its progression or stably reverse its clinical symptoms. AD has become a major health problem in all societies with a high life expectancy and has also become a significant economic burden on the health systems of these societies.

AD的特征在于中枢神经系统(CNS)中的2个主要病理学特征：淀粉样蛋白斑块的出现和神经元纤维缠结(Hardy等，The amyloid hypothesis of Alzheimer′s disease：progress and problems on the road to therapeutics(阿尔兹海默病的淀粉样蛋白假说：通向治疗之路的进展和问题)，Science.2002 Jul 19；297(5580)：353-6，Selkoe，Cellbiology of the amyloid beta-protein precursor and the mechanism of Alzheimer′s disease(淀粉样蛋白β-蛋白前体的细胞生物学和阿尔茨海默病的机制)，Annu Rev CellBiol.1994；10：373-403)。在唐氏综合征(三体性21)患者中通常也发现有这两个病理学特征，其在早期也显示出类似AD的症状。神经元纤维缠结是微管结合蛋白τ(MAPT)的细胞内聚集体。淀粉样蛋白斑块出现在细胞外空间；它们的主要组分为Aβ-肽。后者是源于β-淀粉样蛋白前体蛋白(APP)经由一系列蛋白裂解步骤的一组蛋白水解片段。已经识别出数种形式的APP，其中最大量的是695、751和770个氨基酸长度的蛋白质。它们都经由不同的剪接产生于一个基因。Aβ-肽源自与APP相同的结构域但是在它们的N-和C-端不同，主要物种为40和42个氨基酸长度。存在强烈暗示聚集的Aβ-肽是AD的发病机理中的必需分子的数个证据链：1)由Aβ-肽形成的淀粉样蛋白斑块是AD病理学的不变部分；2)Aβ-肽对于神经元是有毒的；3)在家族性阿尔茨海默病(FAD)中，在致病基因APP、PSN1、PSN2上的突变导致增加水平的Aβ-肽和早期大脑淀粉样变性；4)表达了这种FAD基因的转基因小鼠展现出带有与人类疾病的很多类似处的病理学。Aβ-肽由APP通过名为β-和γ-分泌酶的2个蛋白水解酶的连续作用产生。β-分泌酶首先将在跨膜结构域(TM)外侧大约28个氨基酸的APP细胞外结构域断开以产生含有TM-和细胞质结构域(CTFβ)的APP的C端片段。CTFβ是γ-分泌酶的底物，其在TM内的数个相邻位置裂解以产生Aβ肽和细胞质片段。γ-分泌酶是至少4个不同蛋白质的复合物，其催化亚基非常类似早老素蛋白(PSEN1、PSEN2)。β-分泌酶(BACE1，Asp2；BACE表示β位APP裂解酶)是通过跨膜结构域锚定至膜中的天冬氨酰蛋白酶(Vassar等，Beta-secretasecleavage of Alzheimer′s amyloid precursor protein by the transmembraneaspartic protease BACE(跨膜天冬氨酸蛋白酶BACE对阿尔茨海默淀粉样蛋白前体蛋白的β-分泌酶裂解)，Science.1999 Oct 22；286(5440)：735)。它在人类机体的很多组织中被表达，但是其水平在CNS中尤其高。BACE1基因在小鼠中的基因消除清楚地显示其活性对于导致Aβ-肽的产生的APP加工是重要的，在没有BACE1的情况下不产生Aβ-肽(Luo等，Micedeficient in BACE1，the Alzheimer′s beta-secretase，have normal phenotype andabolished beta-amyloid generation(阿尔茨海默β-分泌酶，BACE1缺陷小鼠具有正常的表型，并且消除了β-淀粉样蛋白的产生)，Nat Neurosci.2001 Mar；4(3)：231-2，Roberds等，BACE knockout mice are healthy despite lacking the primary beta-secretaseactivity in brain：implications for Alzheimer′s disease therapeutics(BACE基因敲除小鼠是健康的，尽管大脑中缺少基本的β-分泌酶活性：提示阿尔茨海默病的治疗方法)，Hum Mol Genet.2001 Jun 1；10(12)：1317-24)。通过基因工程表达人类APP基因并在衰老过程中形成广泛淀粉样蛋白斑块和阿尔茨海默病样病理学的小鼠，当通过将BACE1等位基因之一基因消除降低β-分泌酶活性时不再表现为这样(McConlogue等，Partialreduction of BACE1 has dramatic effects on Alzheimer plaque and synapticpathology in APP Transgenic Mice (BACE1的部分减少对APP转基因小鼠中的阿尔茨海默斑块和突触病理学具有极大的影响).J Biol Chem.2007 Sep 7；282(36)：26326)。因此推测BACE1活性的抑制剂可以是可用于阿尔茨海默病(AD)治疗干预的药剂。已经提交了一些描述各种结果的BACE 1抑制剂的专利申请，例如WO2009103626，WO2010128058，WO2011020806，WO2011029803，WO2011069934，WO2011070029，WO2011138293，WO2012019966，WO2012028563，WO2012098064，WO2012104263，WO2012107371，WO2012110459，WO2012119883，WO2012126791，WO2012136603，WO2012139993，WO2012156284，WO2012163790，WO2012168164，WO2012168175，WO2013004676，WO2013041499，WO2013110622，WO2013174781，WO2014001228，WO2014114532，WO2014150331，WO2014150340，WO2014059185和WO2014150344。AD is characterized by two main pathological features in the central nervous system (CNS): the appearance of amyloid plaques and neurofibrillary tangles (Hardy et al., The amyloid hypothesis of Alzheimer's disease: progress and problems on the road to therapeutics, Science. 2002 Jul 19;297(5580):353-6, Selkoe, Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease, Annu Rev Cell Biol. 1994;10:373-403). These two pathological features are also commonly found in patients with Down syndrome (trisomy 21), who also show symptoms similar to AD at an early stage. Neurofibrillary tangles are intracellular aggregates of the microtubule-associated protein tau (MAPT). Amyloid plaques appear in the extracellular space; their primary component is Aβ-peptide. The latter is a group of proteolytic fragments derived from the β-amyloid precursor protein (APP) through a series of proteolytic cleavage steps. Several forms of APP have been identified, the most abundant of which are proteins of 695, 751, and 770 amino acids in length. They are all produced from a single gene through alternative splicing. Aβ-peptide originates from the same structural domain as APP but differs at their N- and C-termini, with the predominant species being 40 and 42 amino acids in length. Several lines of evidence strongly suggest that aggregated Aβ-peptide is an essential molecule in the pathogenesis of AD: 1) Amyloid plaques formed by Aβ-peptide are an invariant part of AD pathology; 2) Aβ-peptide is toxic to neurons; 3) In familial Alzheimer's disease (FAD), mutations in the causative genes APP, PSN1, and PSN2 lead to increased levels of Aβ-peptide and early cerebral amyloidosis; and 4) transgenic mice expressing the FAD gene exhibit a pathology with many similarities to the human disease. Aβ-peptides are produced from APP by the sequential action of two proteolytic enzymes, named β- and γ-secretases. β-secretase first cleaves the APP extracellular domain of approximately 28 amino acids outside the transmembrane domain (TM) to produce a C-terminal fragment of APP containing the TM- and cytoplasmic domains (CTFβ). CTFβ is a substrate for γ-secretase, which cleaves at several adjacent positions within the TM to produce Aβ peptides and cytoplasmic fragments. γ-secretase is a complex of at least four different proteins, the catalytic subunits of which are very similar to the presenilin proteins (PSEN1, PSEN2). β-Secretase (BACE1, Asp2; BACE stands for β-site APP cleaving enzyme) is an aspartyl protease anchored to the membrane via a transmembrane domain (Vassar et al., Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE, Science. 1999 Oct 22; 286(5440): 735). It is expressed in many tissues of the human body, but its levels are particularly high in the central nervous system (CNS). Genetic ablation of the BACE1 gene in mice clearly showed that its activity is important for APP processing leading to the production of Aβ-peptide, which is not produced in the absence of BACE1 (Luo et al., Mice deficient in BACE1, the Alzheimer's beta-secretase, have normal phenotype and abolished beta-amyloid generation. Nat Neurosci. 2001 Mar; 4(3): 231-2, Roberds et al., BACE knockout mice are healthy despite lacking the primary beta-secretase activity in brain: implications for Alzheimer's disease therapeutics. Hum Mol Genet. 2001 Jun 1; 10(12): 1317-24). Mice that were genetically engineered to express the human APP gene and develop extensive amyloid plaques and Alzheimer's disease-like pathology during aging no longer display this behavior when β-secretase activity is reduced by genetically eliminating one of the BACE1 alleles (McConlogue et al., Partial reduction of BACE1 has dramatic effects on Alzheimer plaque and synaptic pathology in APP Transgenic Mice. J Biol Chem. 2007 Sep 7; 282(36): 263-26). It is therefore speculated that inhibitors of BACE1 activity may be agents that can be used for therapeutic intervention in Alzheimer's disease (AD). Several studies have been submitted describing various results on BACE1. Patent applications for inhibitors of 1, such as WO2009103626, WO2010128058, WO2011020806, WO2011029803, WO2011069934, WO2011070029, WO2011138293, WO2012019966, WO2012028563, WO2012098064, WO2012104263, WO2012107371, WO2012110459, WO2012119883, WO2012126791, WO2012136603, WO2012139993, WO2012156284, WO2012163790, WO2012168164, WO2012168175, WO2013004676, WO2013041499, WO2013110622, WO2013174781, WO2014001228, WO2014114532, WO2014150331, WO2014150340, WO2014059185 and WO2014150344.

此外，β-淀粉样肽在神经组织(例如，大脑)之中，之上或周围的形成或者形成和沉积被本发明的化合物抑制，即抑制由APP或APP片段产生Aβ。Furthermore, the formation or formation and deposition of beta-amyloid peptide in, on or around neural tissue (eg, brain) is inhibited by the compounds of the present invention, ie the production of A[beta] from APP or APP fragments is inhibited.

本发明提供式I的新化合物，它们的制备，基于根据本发明的化合物的药物以及它们的制备，以及式I化合物在控制或防止疾病如阿尔茨海默病中的用途。The present invention provides novel compounds of formula I, their preparation, medicaments based on the compounds according to the invention and their preparation, and the use of compounds of formula I in controlling or preventing diseases such as Alzheimer's disease.

发明领域Field of the Invention

本发明提供具有BACE1抑制性质的S-亚氨基S-氧代亚氨基噻嗪类化合物、它们的制备、包含它们的药物组合物及它们作为治疗活性物质的用途。The present invention provides S-imino S-oxoiminothiazines having BACE1 inhibitory properties, their preparation, pharmaceutical compositions containing them and their use as therapeutically active substances.

发明概述SUMMARY OF THE INVENTION

本发明提供一种式I化合物，The present invention provides a compound of formula I,

其中取代基和变量如以下和权利要求中所述，或其药用盐。wherein substituents and variables are as described below and in the claims, or a pharmaceutically acceptable salt thereof.

本发明的化合物具有Asp2(β-分泌酶，BACE1或膜天冬氨酸蛋白酶-2(Memapsin-2))抑制活性并且因此可以在特征在于升高的β-淀粉样蛋白水平和/或β-淀粉样蛋白低聚物和/或β-淀粉样蛋白斑以及进一步的沉积物的疾病和病症，尤其是阿尔茨海默病的治疗性和/或预防性治疗中使用。The compounds of the present invention have Asp2 (β-secretase, BACE1 or Memapsin-2) inhibitory activity and can therefore be used in the therapeutic and/or prophylactic treatment of diseases and disorders characterized by elevated β-amyloid levels and/or β-amyloid oligomers and/or β-amyloid plaques and further deposits, in particular Alzheimer's disease.

发明详述Detailed Description of the Invention

本发明提供式I化合物以及它们的药用盐，上述化合物的制备，含有它们的药物及其制备，以及上述化合物在治疗性和/或预防性治疗与抑制BACE1相关的疾病和病症，如阿尔茨海默病中的用途。此外，本发明的化合物通过抑制由APP或APP片段产生Aβ来抑制β-淀粉样蛋白斑在神经组织(例如，大脑)中、上或周围的形成或形成和沉积。The present invention provides compounds of formula I and their pharmaceutically acceptable salts, preparations of the above compounds, medicaments containing them and their preparations, and uses of the above compounds in the therapeutic and/or prophylactic treatment of diseases and conditions associated with the inhibition of BACE1, such as Alzheimer's disease. In addition, the compounds of the present invention inhibit the formation or formation and deposition of β-amyloid plaques in, on, or around neural tissue (e.g., the brain) by inhibiting the production of Aβ by APP or APP fragments.

不管被讨论的术语单独或与其他基团组合地出现，下列对本说明书中使用的一般术语的定义均适用。The following definitions of the general terms used in this specification apply regardless of whether the term in question appears alone or in combination with other groups.

除非另外指明，则本申请中使用的下列术语，包括说明书和权利要求书，均具有下面给出的定义。必须指出的是，当在说明书和所附权利要求中使用时，单数形式“一个”、“一种”和“所述”包括复数指代物，除非上下文另外明确地规定。Unless otherwise indicated, the following terms used in this application, including the specification and claims, have the definitions given below. It must be noted that, when used in the specification and the appended claims, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise.

术语“C_1-6-烷基”，单独或与其它基团组合，表示烃基，所述烃基可以是直链或具有单个或多个分支的支链，其中所述烷基基团通常包含1至6个碳原子，例如，甲基(Me)、乙基(Et)、丙基、异丙基(i-丙基)、正丁基、i-丁基(异丁基)、2-丁基(仲丁基)、t-丁基(叔丁基)、异戊基、2-乙基-丙基(2-甲基-丙基)、1，2-二甲基-丙基等。特别的“C_1-6-烷基”是“C_1-3-烷基”。具体基团是甲基和乙基。最具体的基团是甲基。The term "Ci _-6 -alkyl", alone or in combination with other groups, denotes a hydrocarbon radical which may be straight-chain or branched with one or more branches, wherein the alkyl radical generally contains 1 to 6 carbon atoms, for example, methyl (Me), ethyl (Et), propyl, isopropyl (i-propyl), n-butyl, i-butyl (isobutyl), 2-butyl (sec-butyl), t-butyl (tert-butyl), isopentyl, 2-ethyl-propyl (2-methyl-propyl), 1,2-dimethyl-propyl, etc. A particular "Ci _-6 -alkyl" is "Ci _-3 -alkyl". Specific groups are methyl and ethyl. The most specific group is methyl.

术语“卤素-C_1-6-烷基”或“C_1-6-烷基-卤素”，单独或与其它基团组合，是指如本文所定义的C_1-6-烷基，其被一个或多个卤素，特别是1-5个卤素，更特别是1-3个卤素取代。特别的卤素是氟，特别的“卤素-C_1-6-烷基”是氟-C_1-6-烷基并且特别的“卤素-C_1-3-烷基”是氟-C_1-3-烷基。实例是三氟甲基、二氟甲基、氟甲基等。具体的基团是氟甲基。The term "halogen- _{Ci_6} -alkyl" or " _{Ci_6} -alkyl-halogen", alone or in combination with other groups, refers to _{Ci_6} -alkyl as defined herein, which is substituted by one or more halogens, particularly 1 to 5 halogens, more particularly 1 to 3 halogens. Particular halogen is fluorine, particular "halogen- _{Ci_6} -alkyl" is fluoro- _{Ci_6} -alkyl and particular "halogen- _{Ci_3} -alkyl" is fluoro- _{Ci_3} -alkyl. Examples are trifluoromethyl, difluoromethyl, fluoromethyl, etc. A specific group is fluoromethyl.

术语“氰基”，单独或与其它基团组合，是指N≡C-(NC-)。The term "cyano", alone or in combination with other groups, refers to N≡C-(NC-).

术语“卤素”，单独或与其它基团组合，表示氯(Cl)、碘(I)、氟(F)和溴(Br)。特别的“卤素”是Cl、I和F。具体基团是F。The term "halogen", alone or in combination with other groups, denotes chlorine (Cl), iodine (I), fluorine (F) and bromine (Br). Particular "halogen" are Cl, I and F. A specific group is F.

术语“杂芳基”，单独或与其它基团组合，是指这样的芳香碳环基团，其具有单个4至8元环，尤其是5至8元环，或包含6至14个，尤其是6至10个环原子的多个稠合环，并且含有1、2或3个独立地选自N、O和S的杂原子，尤其是1N或2N，在所述基团中至少一个杂环是芳香性的。“杂芳基”的实例包括苯并呋喃基、苯并咪唑基、1H-苯并咪唑基、苯并嗪基、苯并唑基、苯并噻嗪基、苯并噻唑基、苯并噻吩基、苯并三唑基、呋喃基、咪唑基、吲唑基、1H-吲唑基、吲哚基、异喹啉基、异噻唑基、异唑基、唑基、吡嗪基、吡唑基(pyrazolyl)(吡唑基(pyrazyl))、1H-吡唑基、吡唑并[1，5-a]吡啶基、哒嗪基、吡啶基(pyridinyl)(吡啶基(pyridyl))、嘧啶基(pyrimidinyl)(嘧啶基(pyrimidyl))、吡咯基、喹啉基、四唑基、噻唑基、噻吩基、三唑基、6，7-二氢-5H-[1]氮茚基等。特别的″杂芳基″是吡啶基，吡嗪基和咪唑并[1，2-a]吡啶基。The term "heteroaryl", alone or in combination with other groups, refers to an aromatic carbocyclic group having a single 4- to 8-membered ring, especially a 5- to 8-membered ring, or a plurality of fused rings containing 6 to 14, especially 6 to 10, ring atoms, and containing 1, 2 or 3 heteroatoms independently selected from N, O and S, especially 1N or 2N, in which group at least one heterocycle is aromatic. Examples of "heteroaryl" include benzofuranyl, benzimidazolyl, 1H-benzimidazolyl, benzoxazinyl, benzoxazolyl, benzothiazinyl, benzothiazolyl, benzothienyl, benzotriazolyl, furanyl, imidazolyl, indazolyl, 1H-indazolyl, indolyl, isoquinolinyl, isothiazolyl, isoxazolyl, oxazolyl, pyrazinyl, pyrazolyl (pyrazolyl), 1H-pyrazolyl, pyrazolo [1,5-a] pyridinyl, pyridazinyl, pyridinyl (pyridyl), pyrimidinyl (pyrimidyl), pyrrolyl, quinolinyl, tetrazolyl, thiazolyl, thienyl, triazolyl, 6,7-dihydro-5H- [1] indolyl, and the like. Particular "heteroaryl" groups are pyridinyl, pyrazinyl and imidazo[1,2-a]pyridinyl.

术语“芳基”是指包含6至10个碳环原子的单价芳族碳环单环或二环的环体系。芳基部分的实例包括苯基和萘基。特别的“芳基”是苯基。The term "aryl" refers to a monovalent aromatic carbocyclic monocyclic or bicyclic ring system containing 6 to 10 carbon ring atoms. Examples of aryl moieties include phenyl and naphthyl. A particular "aryl" group is phenyl.

术语“药用盐”是指适合与人类和动物的组织接触使用的盐。与无机和有机酸的合适的盐的实例为，但是不限于：乙酸、柠檬酸、甲酸、富马酸、盐酸、乳酸、马来酸、苹果酸、甲磺酸、硝酸、磷酸、对甲苯磺酸、琥珀酸、硫磺酸(硫酸)、酒石酸、三氟乙酸等。具体的酸是甲酸、三氟乙酸和盐酸。具体的酸是三氟乙酸。The term "pharmaceutically acceptable salt" refers to salts suitable for use in contact with human and animal tissues. Examples of suitable salts with inorganic and organic acids include, but are not limited to, acetic acid, citric acid, formic acid, fumaric acid, hydrochloric acid, lactic acid, maleic acid, malic acid, methanesulfonic acid, nitric acid, phosphoric acid, p-toluenesulfonic acid, succinic acid, sulfuric acid (sulfuric acid), tartaric acid, trifluoroacetic acid, and the like. Specific acids include formic acid, trifluoroacetic acid, and hydrochloric acid. A specific acid is trifluoroacetic acid.

术语“氨基”，单独或与其他基团组合，是指-NH₂。The term "amino", alone or in combination with other groups, refers to _-NH2 .

术语“羟基”或“羟基”，单独或与其他基团组合，是指-OH。The term "hydroxy" or "hydroxyl group", alone or in combination with other groups, refers to -OH.

术语“C_2-6-炔基-C_1-6-烷氧基”，单独或与其他基团组合，是指如本文中所定义的C_1-6-烷氧基，其被一个或多个如本文中所定义的C_2-6-炔基，尤其是1个C_2-6-炔基取代。The term "C _2-6 -alkynyl-C _1-6 -alkoxy", alone or in combination with other groups, refers to a C _1-6 -alkoxy group as defined herein, which is substituted by one or more C _2-6 -alkynyl groups, in particular 1 C _2-6 -alkynyl group, as defined herein.

术语“C_2-6-炔基”，单独或与其他基团组合，表示2至6个碳原子，尤其是2至4个碳原子的并且包含一个、两个或三个三碱的单价直链或支链饱和烃基。C_2-6-炔基的实例包括乙炔基，丙炔基，和正丁炔基。The term "C _2-6 -alkynyl", alone or in combination with other groups, means a monovalent straight-chain or branched saturated hydrocarbon radical of 2 to 6 carbon atoms, in particular 2 to 4 carbon atoms, and containing one, two or three tribasic groups. Examples of C _2-6 -alkynyl include ethynyl, propynyl, and n-butynyl.

术语“C_1-6-烷氧基-C_1-6-烷基”，单独或与其他基团组合，是指如本文所定义的C_1-6-烷基，其被一个或多个如本文所述的C_1-6-烷氧基，特别是1个C_1-6-烷氧基取代。特别的“C_1-6-烷氧基-C_1-6-烷基”是甲氧基-C_1-6-烷基。实例是甲氧基甲基，甲氧基乙基等。The term " _{Ci_6} -alkoxy- _{Ci_6} -alkyl", alone or in combination with other groups, refers to a _{Ci_6} -alkyl group as defined herein, substituted by one or more _{Ci_6} -alkoxy groups as described herein, in particular one _{Ci_6} -alkoxy group. A particular " _{Ci_6} -alkoxy- _{Ci_6} -alkyl" is methoxy- _{Ci_6} -alkyl. Examples are methoxymethyl, methoxyethyl, and the like.

术语“C_3-6-环烷基”是指3至8元碳环，例如环丙基，环丁基，环戊基，环己基，环庚基或环辛基。特别的是具有3，4，5或6元碳环的环烷基。具体是环丙基。The term "C _3-6 -cycloalkyl" refers to a 3- to 8-membered carbon ring, for example cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl or cyclooctyl. In particular, it is a cycloalkyl group having 3, 4, 5 or 6 carbon ring members. In particular, it is cyclopropyl.

术语″C_1-6-烷氧基″，单独或与其他基团组合，代表-O-C_1-6-烷基，其可以是直连或支链的，具有单个或多个分支，其中所述烷基通常包含1至6个碳原子，例如，甲氧基(OMe，MeO)，乙氧基(OEt)，丙氧基，异丙氧基(i-丙氧基)，正丁氧基，i-丁氧基(异丁氧基)，2-丁氧基(仲丁氧基)，t-丁氧基(叔丁氧基)，异戊氧基(i-戊氧基)等。特别的“C_1-6-烷氧基”是具有1至4个碳原子的基团。具体的是乙氧基和甲氧基。The term "C _1-6 -alkoxy", alone or in combination with other groups, represents -OC _1-6 -alkyl, which can be straight or branched, with single or multiple branches, wherein the alkyl group generally contains 1 to 6 carbon atoms, for example, methoxy (OMe, MeO), ethoxy (OEt), propoxy, isopropoxy (i-propoxy), n-butoxy, i-butoxy (isobutoxy), 2-butoxy (sec-butoxy), t-butoxy (tert-butoxy), isopentyloxy (i-pentyloxy) and the like. Particular "C _1-6 -alkoxy" is a group having 1 to 4 carbon atoms. Specific are ethoxy and methoxy.

术语“卤素-C_1-6-烷氧基”，单独或与其他基团组合，是指如本文中所述的C_1-6-烷氧基，其被一个或多个卤素，尤其是氟取代。具体的“卤素-C_1-6-烷氧基”是氟-C_1-6-烷氧基。特别的“卤素-C_1-6-烷氧基”是CHF₂-CF₂-CH₂-O-，CHF₂-O-和CF₂-O-。The term "halogen- _{Ci_6} -alkoxy", alone or in combination with other groups, refers to a _C1-6 -alkoxy group as described herein, which is substituted by one or more halogens, especially fluorine. A specific "halogen- _{Ci_6} -alkoxy group" is fluoro- _{Ci_6} -alkoxy. Particular "halogen- _{Ci_6} -alkoxy" groups are _CHF2 - _CF2 -CH2 _- O-, CHF2 _- O- and CF2 _- O-.

术语“药用载体”和“药用辅助物质”是指与制剂的其他成分相容的载体和辅助物质如稀释剂或赋形剂。The terms "pharmaceutically acceptable carrier" and "pharmaceutically acceptable auxiliary substance" refer to carriers and auxiliary substances such as diluents or excipients that are compatible with the other ingredients of the formulation.

术语“药物组合物”包括包含预定量或比例的特定成分的产品，以及通过组合特定量的特定成分直接地或间接地得到的任何产品。尤其是，它包括包含一种或多种活性组分，和任选的包含惰性成分的载体的产品，以及由任何两种以上的成分的组合、复合或聚集，或者由一种或多种成分的分解，或由一种或多种成分的其他类型的反应或相互作用直接地或间接地得到的任何产物。The term "pharmaceutical composition" includes a product comprising specified ingredients in predetermined amounts or proportions, as well as any product resulting, directly or indirectly, from combining the specified ingredients in the specified amounts. In particular, it includes a product comprising one or more active ingredients, and optionally a carrier comprising an inert ingredient, as well as any product resulting, directly or indirectly, from the combination, complexation or aggregation of any two or more ingredients, or from the decomposition of one or more ingredients, or from some other type of reaction or interaction of one or more ingredients.

术语“抑制剂”表示与特定受体的特定配体竞争、减少或防止该特定受体与该特定配体的结合或者减少或防止特定蛋白的功能的抑制的化合物。The term "inhibitor" refers to a compound that competes with a specific ligand for a specific receptor, reduces or prevents the binding of the specific receptor to the specific ligand, or reduces or prevents the inhibition of the function of a specific protein.

术语“半最大抑制浓度”(IC₅₀)是指在体外获得生物过程的50％抑制所需的特定化合物的浓度。可以将IC₅₀值对数地转换为pIC₅₀值(-log IC₅₀)，其中较大的值表示指数地增加的潜力。IC₅₀值不是绝对值而依赖于试验条件例如所采用的浓度。可以将IC₅₀值使用Cheng-Prusoff公式(Biochem.Pharmacol.(1973)22：3099)转换为绝对抑制常数(Ki)。术语“抑制常数”(Ki)是指特定抑制剂对受体的绝对结合亲和性。其使用竞争结合测定测量，并且等于如果不存在竞争配体(例如放射性配体)特定抑制剂将占据受体的50％的情况下的浓度。可以将Ki值对数地转换为pKi值(-logKi)，其中较大的值表示指数地增加的潜力。The term "half-maximal inhibitory concentration" ( _IC50 ) refers to the concentration of a particular compound required to achieve 50% inhibition of a biological process in vitro. _IC50 values can be converted logarithmically to _pIC50 values (-log _IC50 ), where larger values indicate exponentially greater potency. _IC50 values are not absolute values but depend on the experimental conditions, such as the concentration employed. _IC50 values can be converted to an absolute inhibition constant (Ki) using the Cheng-Prusoff equation (Biochem. Pharmacol. (1973) 22:3099). The term "inhibition constant" (Ki) refers to the absolute binding affinity of a particular inhibitor for a receptor. It is measured using a competition binding assay and is equal to the concentration at which the particular inhibitor would occupy 50% of the receptor in the absence of a competing ligand (e.g., a radioligand). Ki values can be converted logarithmically to pKi values (-logKi), where larger values indicate exponentially greater potency.

“治疗有效量”意指当被给药于受试者用于治疗疾病状态时，足以实现对于疾病状态的这种治疗的化合物的量。“治疗有效量”将依赖于化合物、所治疗的疾病状态、所治疗的疾病的严重性、受试者的年龄和相对健康状况、给药的路线和形式、主治医师或兽医师的判断以及其他因素而变化。"Therapeutically effective amount" means the amount of a compound that, when administered to a subject for treating a disease state, is sufficient to effect such treatment for the disease state. The "therapeutically effective amount" will vary depending on the compound, the disease state being treated, the severity of the disease being treated, the age and relative health of the subject, the route and form of administration, the judgment of the attending physician or veterinarian, and other factors.

术语“如本文所定义的”和“如本文所描述的”当涉及变量时通过引用结合变量的宽泛定义以及如果有的话，具体的、更具体的和最具体的定义。The terms "as defined herein" and "as described herein" when referring to a variable incorporate by reference the broad definition of the variable as well as specific, more specific and most specific definitions, if any.

当涉及化学反应时术语“处理”、“接触”和“反应”意指在合适的条件下加入或混合两种以上的试剂以制备所示和/或所需的产物。应该明白产生所示和/或所需产物的反应可能不一定直接得自最初加入的两种试剂的组合，即，在混合物中可能产生最终导致所示和/或所需产物的形成的一种或多种中间体。The terms "treating," "contacting," and "reacting" when referring to a chemical reaction mean adding or mixing two or more reagents under appropriate conditions to produce the indicated and/or desired product. It should be understood that the reaction that produces the indicated and/or desired product may not necessarily result directly from the combination of the two reagents initially added, that is, one or more intermediates may be produced in the mixture that ultimately lead to the formation of the indicated and/or desired product.

术语“芳族的”是指如文献中所定义的芳香性的传统概念，尤其是在IUPAC-Compendium of Chemical Terminology，2nd，A.D.McNaught和A.Wilkinson(编辑).Blackwell Scientific Publications，Oxford(1997)中所定义。The term "aromatic" refers to the traditional concept of aromaticity as defined in the literature, in particular in IUPAC-Compendium of Chemical Terminology, 2nd, A.D. McNaught and A. Wilkinson (eds.). Blackwell Scientific Publications, Oxford (1997).

术语“药用赋形剂”是指在配制药物产品中使用的不具有治疗活性并且无毒的任意成分，如崩解剂、粘合剂、填充剂、溶剂、缓冲剂、张度剂、稳定剂、抗氧化剂、表面活性剂或润滑剂。The term "pharmaceutically acceptable excipient" refers to any ingredient that is not therapeutically active and non-toxic and is used in formulating a pharmaceutical product, such as a disintegrant, binder, filler, solvent, buffer, tonicity agent, stabilizer, antioxidant, surfactant, or lubricant.

当在化学结构中存在手性碳时，意图是该结构包括作为纯立体异构体的与该手性碳相关的所有立体异构体以及其混合物。When a chiral carbon is present in a chemical structure, it is intended that the structure encompass all stereoisomers associated with that chiral carbon as pure stereoisomers, as well as mixtures thereof.

本发明还提供药物组合物、使用上述化合物的方法和制备上述化合物的方法。The present invention also provides pharmaceutical compositions, methods of using the above compounds, and methods of preparing the above compounds.

所有单独的实施方案可以进行组合。All individual embodiments can be combined.

本发明的一个实施方案提供式I化合物，One embodiment of the present invention provides a compound of formula I,

其中in

n是1，2或3；n is 1, 2, or 3;

R¹选自由以下各项组成的组 ^R1 is selected from the group consisting of

i)C_1-6-烷基，和i) C _1-6 -alkyl, and

ii)卤素-C_1-6-烷基；ii) halogen-C _1-6 -alkyl;

R²选自由以下各项组成的组 ^R2 is selected from the group consisting of

i)C_1-6-烷基，和i) C _1-6 -alkyl, and

ii)卤素-C_1-6-烷基；ii) halogen-C _1-6 -alkyl;

或R¹和R²与它们连接的C-原子一起形成C_3-6-环烷基-，其中所述C_3-6-环烷基-任选地被一个或多个选自由卤素和羟基组成的组的取代基取代；or R ¹ and R ² together with the C-atom to which they are attached form a C _3-6 -cycloalkyl-, wherein said C _3-6 -cycloalkyl- is optionally substituted with one or more substituents selected from the group consisting of halogen and hydroxy;

R³各自独立地选自由以下各项组成的组 ^R3 are each independently selected from the group consisting of

i)氢，i) Hydrogen,

ii)C_1-6-烷基，和ii) C _1-6 -alkyl, and

iii)卤素；iii) halogen;

R⁴各自独立地选自由以下各项组成的组R ⁴ is each independently selected from the group consisting of

i)氢，i) Hydrogen,

ii)C_1-6-烷基，和ii) C _1-6 -alkyl, and

iii)卤素；iii) halogen;

或其中R³和R⁴一起是-(CH₂)_m-，其中m是2，3，4或5，or wherein R ³ and R ⁴ together are -(CH ₂ ) _m -, wherein m is 2, 3, 4 or 5,

R⁵是氢。 ^R5 is hydrogen.

R⁶选自由以下各项组成的组R ⁶ is selected from the group consisting of

i)C_1-6-烷基，和i) C _1-6 -alkyl, and

ii)卤素-C_1-6-烷基；ii) halogen-C _1-6 -alkyl;

R⁷选自由以下各项组成的组R ⁷ is selected from the group consisting of

i)氢，和i) hydrogen, and

ii)卤素；ii) halogen;

R⁸选自由以下各项组成的组R ⁸ is selected from the group consisting of

i)芳基，i) aryl,

ii)被1-4个单独选自以下各项的取代基取代的芳基：氨基，氰基，卤素，卤素-C_1-6-烷基，卤素-C_1-6-烷氧基，C_1-6-烷氧基，C_1-6-烷氧基-C_1-6-烷基，C_2-6-炔基-C_1-6-烷氧基，C_2-6-炔基，C_1-6-烷基，其中R⁹是H或C_1-6-烷基的COOR⁹，其中R¹⁰是H或C_1-6-烷基C_3-6-环烷基并且R¹¹是H或C_1-6-烷基的CONR¹⁰R¹¹，任选地被1至4个单独选自由卤素，氰基，C_1-6-烷基和C_1-6-烷氧基组成的组的取代基取代的C_3-6-环烷基，C_3-6-环烷基-C_1-6-烷氧基和C_3-6-环烷基-C_1-6-烷氧基，其中所述环烷基单元被1至4个单独选自由卤素，氰基，C_1-6-烷基和C_1-6-烷氧基组成的组的取代基取代；ii) aryl substituted with 1 to 4 substituents individually selected from the group consisting of amino, cyano, halogen, halogen-Ci _-6 -alkyl, halogen-Ci _-6 -alkoxy, Ci _-6 -alkoxy, Ci _- 6-alkoxy-Ci _-6- alkyl, _C2-6 -alkynyl-Ci- ₆ -alkoxy, _C2-6 -alkynyl, Ci _-6 -alkyl, ^COOR9 where ^R9 is H or Ci _-6 -alkyl, CONR10R11 where ^R10 is H or Ci _- ₆ -alkylC3-6-cycloalkyl and ^R11 is H or Ci _-6 ^- alkyl, _C3-6 -cycloalkyl, ^C3-6 -cycloalkyl-Ci _-6 -alkyl optionally substituted with ₁ to 4 substituents individually selected from the group consisting of halogen, cyano, Ci _-6 -alkyl and Ci _-6- alkoxy -alkoxy and C _3-6 -cycloalkyl-C _1-6 -alkoxy, wherein the cycloalkyl unit is substituted by 1 to 4 substituents individually selected from the group consisting of halogen, cyano, C _1-6 -alkyl and C _1-6 -alkoxy;

iii)杂芳基，和iii) heteroaryl, and

iv)被1-4个单独选自以下各项的取代基取代的杂芳基：氨基，氰基，卤素，卤素-C_1-6-烷基，卤素-C_1-6-烷氧基，C_1-6-烷氧基，C_1-6-烷氧基-C_1-6-烷基，C_2-6-炔基-C_1-6-烷氧基，C_2-6-炔基，C_1-6-烷基，其中R⁹是H或C_1-6-烷基的COOR⁹，其中R¹⁰是H或C_1-6-烷基C_3-6-环烷基并且R¹¹是H或C_1-6-烷基的CONR¹⁰R¹¹，任选地被1至4个单独选自由卤素，氰基，C_1-6-烷基和C_1-6-烷氧基组成的组的取代基取代的C_3-6-环烷基，C_3-6-环烷基-C_1-6-烷氧基和C_3-6-环烷基-C_1-6-烷氧基，其中所述环烷基单元被1至4个单独选自由卤素，氰基，C_1-6-烷基和C_1-6-烷氧基组成的组的取代基取代；iv) heteroaryl substituted with 1 to 4 substituents individually selected from the group consisting of amino, cyano, halogen, halogen-Ci _-6 -alkyl, halogen-Ci _-6 -alkoxy, Ci _-6 -alkoxy, Ci _-6 -alkoxy-Ci _-6- alkyl, _C2-6 -alkynyl-Ci- ₆ -alkoxy, _C2-6 -alkynyl, Ci _-6 -alkyl, ^COOR9 where ^R9 is H or Ci _-6 -alkyl, CONR10R11 where ^R10 is H or Ci- ₆ - _alkylC3-6 -cycloalkyl and ^R11 is H or Ci _-6 -alkyl, ^C3-6 -cycloalkyl, ^C3-6 _- cycloalkyl-Ci _-6 -alkyl optionally substituted with 1 _to 4 substituents individually selected from the group consisting of halogen, cyano, Ci _-6 -alkyl and Ci _-6- alkoxy -alkoxy and C _3-6 -cycloalkyl-C _1-6 -alkoxy, wherein the cycloalkyl unit is substituted by 1 to 4 substituents individually selected from the group consisting of halogen, cyano, C _1-6 -alkyl and C _1-6 -alkoxy;

或其药用盐。or a pharmaceutically acceptable salt thereof.

本发明的某些实施方案提供如本文中所述的式I化合物，其中Certain embodiments of the present invention provide a compound of formula I as described herein, wherein

n是1，2或3；n is 1, 2, or 3;

i)C_1-6-烷基，和i) C _1-6 -alkyl, and

ii)卤素-C_1-6-烷基；ii) halogen-C _1-6 -alkyl;

i)C_1-6-烷基，和i) C _1-6 -alkyl, and

ii)卤素-C_1-6-烷基；ii) halogen-C _1-6 -alkyl;

或R¹和R²与它们连接的C-原子一起形成C_3-6-环烷基-，其中C_3-6-环烷基-任选地被一个或多个选自由卤素和羟基组成的组的取代基取代；or R ¹ and R ² together with the C-atom to which they are attached form C _3-6 -cycloalkyl-, wherein C _3-6 -cycloalkyl- is optionally substituted with one or more substituents selected from the group consisting of halogen and hydroxy;

i)氢，i) Hydrogen,

ii)C_1-6-烷基，和ii) C _1-6 -alkyl, and

iii)卤素；iii) halogen;

i)氢，i) Hydrogen,

ii)C_1-6-烷基，和ii) C _1-6 -alkyl, and

iii)卤素；iii) halogen;

R⁵是氢。 ^R5 is hydrogen.

i)C_1-6-烷基，和i) C _1-6 -alkyl, and

ii)卤素-C_1-6-烷基；ii) halogen-C _1-6 -alkyl;

i)氢，和i) hydrogen, and

ii)卤素；ii) halogen;

i)芳基，i) aryl,

ii)被1-4个单独选自以下各项的取代基取代的芳基：氨基，氰基，卤素，卤素-C_1-6-烷基，卤素-C_1-6-烷氧基，C_1-6-烷氧基，C_1-6-烷氧基-C_1-6-烷基，C_2-6-炔基-C_1-6-烷氧基，C_2-6-炔基，C_1-6-烷基，C_3-6-环烷基，任选地被1至4个单独选自由卤素，氰基，C_1-6-烷基和C_1-6-烷氧基组成的组的取代基取代的C_3-6-环烷基，C_3-6-环烷基-C_1-6-烷氧基和C_3-6-环烷基-C_1-6-烷氧基，其中所述环烷基单元被1至4个单独选自由卤素，氰基，C_1-6-烷基和C_1-6-烷氧基组成的组的取代基取代；ii) aryl substituted with 1 to 4 substituents individually selected from the group consisting of amino, cyano, halogen, halogen-Ci- ₆ -alkyl, halogen-Ci _-6 _- alkoxy, Ci- ₆ -alkoxy, Ci _-6 _- alkoxy-Ci _-6 -alkyl, C2-6-alkynyl-Ci _- ₆ -alkoxy, _C2-6 -alkynyl, Ci- ₆ -alkyl, _C3-6 -cycloalkyl, C3-6-cycloalkyl, _C3-6 -cycloalkyl-Ci- ₆ -alkoxy and _C3-6 -cycloalkyl-Ci _-6 _- alkoxy, wherein the cycloalkyl unit is substituted with 1 to 4 substituents individually selected from the group consisting of halogen, cyano, Ci _-6 -alkyl and Ci _-6 -alkoxy;

iii)杂芳基，和iii) heteroaryl, and

iv)被1-4个单独选自以下各项的取代基取代的杂芳基：氨基，氰基，卤素，卤素-C_1-6-烷基，卤素-C_1-6-烷氧基，C_1-6-烷氧基，C_1-6-烷氧基-C_1-6-烷基，C_2-6-炔基-C_1-6-烷氧基，C_2-6-炔基，C_1-6-烷基，C_3-6-环烷基，任选地被1至4个单独选自由卤素，氰基，C_1-6-烷基和C_1-6-烷氧基取代的C_3-6-环烷基，C_3-6-环烷基-C_1-6-烷氧基和C_3-6-环烷基-C_1-6-烷氧基组成的组的取代基，其中所述环烷基单元被1至4个单独选自由卤素，氰基，C_1-6-烷基和C_1-6-烷氧基组成的组的取代基取代；iv) heteroaryl substituted with 1 to 4 substituents individually selected from the group consisting of amino, cyano, halogen, halogen- _{Ci_6} -alkyl, halogen- _{Ci_6} -alkoxy, _{Ci_6} -alkoxy, _{Ci_6} -alkoxy- _{Ci_6} -alkyl, _C2-6 -alkynyl- _{Ci_6} -alkoxy, _C2-6 -alkynyl, _{Ci_6} -alkyl, _C3-6 -cycloalkyl, C3-6-cycloalkyl substituted with 1 to 4 substituents individually selected from the group consisting of halogen, cyano, _{Ci_6} -alkyl and _{Ci_6} -alkoxy, _C3-6 -cycloalkyl- _{Ci_6} -alkoxy and _C3-6 _- cycloalkyl- _{Ci_6} -alkoxy, wherein the cycloalkyl unit is substituted with 1 to 4 substituents individually selected from the group consisting of halogen, cyano, _{Ci_6} -alkyl and _{Ci_6} -alkoxy;

或其药用盐。or a pharmaceutically acceptable salt thereof.

本发明的某实施方案提供如本文中所述的式I化合物，其中n，R¹，R²，R³，R⁴，R⁵，R⁶，R⁷和R⁸如本文中所述并且当n＝1时，则环构型为顺式。A certain embodiment of the invention provides a compound of formula I as described herein, wherein n, ^R1 , ^R2 , ^R3 , ^R4 , ^R5 , ^R6 , ^R7 and ^R8 are as described herein and when n=1, then the ring configuration is cis.

本发明的某实施方案提供如本文中所述的式I化合物，其是式Ia的化合物，其中n，R¹，R²，R³，R⁴，R⁵，R⁶，R⁷和R⁸如本文中所述的A certain embodiment of the present invention provides a compound of formula I as described herein, which is a compound of formula Ia, wherein n, R ¹ , R ² , R ³ , R ⁴ , R ⁵ , R ⁶ , R ⁷ and R ⁸ are as described herein

本发明的某实施方案提供如本文中所述的式I化合物，其中R¹是甲基。A certain embodiment of the invention provides a compound of formula I as described herein, wherein R ¹ is methyl.

本发明的某实施方案提供如本文中所述的式I化合物，其中R²是甲基。A certain embodiment of the invention provides a compound of formula I as described herein, wherein ^R2 is methyl.

本发明的某实施方案提供如本文中所述的式I化合物，其中R³是氢。A certain embodiment of the invention provides a compound of formula I as described herein, wherein R ³ is hydrogen.

本发明的某实施方案提供如本文中所述的式I化合物，其中R⁴是氢。A certain embodiment of the invention provides a compound of formula I as described herein, wherein ^R4 is hydrogen.

本发明的某实施方案提供如本文中所述的式I化合物，其中R³和R⁴一起是-(CH₂)₂-。A certain embodiment of the invention provides a compound of formula I as described herein, wherein R ³ and R ⁴ together are -(CH ₂ ) ₂ -.

本发明的某实施方案提供如本文中所述的式I化合物，其中R⁵是氢。A certain embodiment of the invention provides a compound of formula I as described herein, wherein R ⁵ is hydrogen.

本发明的某实施方案提供如本文中所述的式I化合物，其中R⁶是甲基。A certain embodiment of the invention provides a compound of formula I as described herein, wherein ^R6 is methyl.

本发明的某实施方案提供如本文中所述的式I化合物，其中R⁷是F。A certain embodiment of the invention provides a compound of formula I as described herein, wherein ^R7 is F.

本发明的某实施方案提供如本文中所述的式I化合物，其中R⁸是被1-2个单独选自氰基和卤素的取代基取代的芳基。A certain embodiment of the invention provides a compound of formula I as described herein, wherein R ⁸ is aryl substituted with 1-2 substituents independently selected from cyano and halogen.

本发明的某实施方案提供如本文中所述的式I化合物，其中R⁸是被1-2个单独选自氰基和Cl的取代基取代的苯基。A certain embodiment of the invention provides a compound of formula I as described herein, wherein R ⁸ is phenyl substituted with 1-2 substituents independently selected from cyano and Cl.

本发明的某实施方案提供如本文中所述的式I化合物，其中R⁸是1-2个单独选自氰基，卤素，卤素-C_1-6-烷氧基，卤素-C_1-6-烷基，C_1-6-烷氧基，C_2-6-炔基和C_1-6-烷基的取代基取代的杂芳基。A certain embodiment of the present invention provides a compound of formula I as described herein, wherein ^R8 is heteroaryl substituted with 1-2 substituents independently selected from cyano, halogen, halogen- _C1-6 -alkoxy, halogen- _C1-6 -alkyl, _C1-6 -alkoxy, _C2-6 -alkynyl and _C1-6 -alkyl.

本发明的某实施方案提供如本文中所述的式I化合物，其中R⁸是1-2个单独选自氰基，卤素，卤素-C_1-6-烷氧基，C_1-6-烷氧基和C_1-6-烷基的取代基取代的杂芳基。A certain embodiment of the invention provides a compound of formula I as described herein, wherein R ⁸ is heteroaryl substituted with 1-2 substituents independently selected from cyano, halogen, halogen-C _1-6 -alkoxy, C _1-6 -alkoxy and C _1-6 -alkyl.

本发明的某实施方案提供如本文中所述的式I化合物，其中R⁸是1H-吡唑基，吡啶基，吡嗪基或咪唑并[1，2-a]吡啶基，其被1-2个单独选自氰基，卤素，卤素-C_1-6-烷氧基，卤素-C_1-6-烷基，C_1-6-烷氧基，C_2-6-炔基和C_1-6-烷基的取代基取代。A certain embodiment of the invention provides a compound of formula I as described herein, wherein ^R8 is 1H-pyrazolyl, pyridinyl, pyrazinyl or imidazo[1,2-a]pyridinyl, which is substituted by 1-2 substituents independently selected from cyano, halogen, halogen- _C1-6 -alkoxy, halogen- _C1-6 -alkyl, _C1-6 -alkoxy, _C2-6 -alkynyl and _C1-6 -alkyl.

本发明的某实施方案提供如本文中所述的式I化合物，其中R⁸是吡啶基，吡嗪基或咪唑并[1，2-a]吡啶基，其各自被1-2个单独选自氰基，卤素，卤素-C_1-6-烷氧基，C_1-6-烷氧基和C_1-6-烷基的取代基取代。A certain embodiment of the invention provides a compound of formula I as described herein, wherein ^R8 is pyridinyl, pyrazinyl or imidazo[1,2-a]pyridinyl, each of which is substituted with 1-2 substituents independently selected from cyano, halogen, halogen- _C1-6 -alkoxy, _C1-6 -alkoxy and _C1-6 -alkyl.

本发明的某实施方案提供如本文中所述的式I化合物，其中n是1或2。A certain embodiment of the invention provides a compound of formula I as described herein, wherein n is 1 or 2.

本发明的某实施方案提供如本文中所述的式I化合物，其中n是1或2，R¹是甲基，R²是甲基，R³在每次出现时都是H，R⁴在每次出现时都是H，R⁵是H，R⁶是甲基并且R⁷是F。A certain embodiment of the invention provides a compound of formula I as described herein, wherein n is 1 or ² , ^R1 is methyl, R2 is methyl, ^R3 at each occurrence is H, ^R4 at each occurrence is H, ^R5 is H, ^R6 is methyl and ^R7 is F.

本发明的某实施方案提供如本文中所述的式I化合物，其选自由以下各项组成的组A certain embodiment of the present invention provides a compound of formula I as described herein, which is selected from the group consisting of

N-(6-((3aR，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代(oxido)-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxido-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((3aR，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺，N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide,

N-(6-((3aR，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺，N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide,

N-(6-((3aR，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺，N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide,

N-(6-((3aR，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(氟甲氧基)吡啶酰胺，N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)picolinamide,

N-(6-((3aR，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺，N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide,

N-(6-((3aR，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺，N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide,

N-(6-((3aR，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺，N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺，N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopicinamide,

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺，N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide,

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺，N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide,

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺，N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide,

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(氟甲氧基)吡啶酰胺，N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)picolinamide,

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺，N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide,

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺，N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide,

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺，N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡嗪-2-甲酰胺，N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyrazine-2-carboxamide,

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氟吡啶酰胺，N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoropyridinecarboxamide,

N-(6-((3aR，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(氟甲氧基)吡啶酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)picolinamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡嗪-2-甲酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyrazine-2-carboxamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氟吡啶酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoropyridinecarboxamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-(二氟甲氧基)吡啶酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-(difluoromethoxy)picolinamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3，5-二氯吡啶酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridinecarboxamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-4-氰基苯甲酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-4-cyanobenzamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-2-氯-4-氰基苯甲酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-2-chloro-4-cyanobenzamide,

N-(6-((3aS，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((3aS，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺，N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide,

N-(6-((3aS，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺，N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide,

N-(6-((3aS，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺，N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide,

N-(6-((3aS，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(氟甲氧基)吡啶酰胺，N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)picolinamide,

N-(6-((3aS，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺，N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide,

N-(6-((3aS，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺，N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide,

N-(6-((3aS，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺，N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氟吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoropyridinecarboxamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyanopyridineamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3，5-二氯吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridinecarboxamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氯吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-chloropicolinamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3-氯-5-(二氟甲氧基)-吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-(difluoromethoxy)-picolinamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(2，2-二氟乙氧基)吡嗪-2-甲酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2-difluoroethoxy)pyrazine-2-carboxamide,

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(丙-1-炔-1-基)吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(prop-1-yn-1-yl)picolinamide,

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺，N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3，5-二氯吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridinecarboxamide,

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide,

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide,

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-6-氯-3-甲基咪唑并[1，2-a]吡啶-2-甲酰胺，N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-6-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide,

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide,

N-(6-((4aR，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((4aR，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺，N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((4aR，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3，5-二氯吡啶酰胺，N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridinecarboxamide,

N-(6-((4aR，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺，N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide,

N-(6-((4aR，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺，N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide,

N-(6-((4aR，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-6-氯-3-甲基咪唑并[1，2-a]吡啶-2-甲酰胺，N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-6-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aR，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺，N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide,

N-(6-((4aR，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺，N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide,

N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺，N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3，5-二氯吡啶酰胺，N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridinecarboxamide,

N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺，N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide,

N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺，N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide,

N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-6-氯-3-甲基咪唑并[1，2-a]吡啶-2-甲酰胺，N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-6-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺，N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide,

N-(6-((4aaS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺，N-(6-((4aaS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide,

N-(6-((4aS，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((4aS，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺，N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((4aS，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3，5-二氯吡啶酰胺，N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridinecarboxamide,

N-(6-((4aS，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺，N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide,

N-(6-((4aS，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺，N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopicolinamide,

N-(6-((4aS，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-6-氯-3-甲基咪唑并[1，2-a]吡啶-2-甲酰胺，N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-6-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aS，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺，N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide,

N-(6-((4aS，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺，N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide,

N-(6-((5aR，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂(thiazepin)-6-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((5aR，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺，N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide,

N-(6-((5aR，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺，N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide,

N-(6-((5aR，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺，N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide,

N-(6-((5aR，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-(氟甲氧基)吡啶酰胺，N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)picolinamide,

N-(6-((5aR，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺，N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide,

N-(6-((5aR，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺，N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide,

N-(6-((5aR，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺，N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((5aR，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((5aR，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺，N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide,

N-(6-((5aR，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺，N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide,

N-(6-((5aR，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺，N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide,

N-(6-((5aR，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-(氟甲氧基)吡啶酰胺，N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)picolinamide,

N-(6-((5aR，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺，N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide,

N-(6-((5aR，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺，N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide,

N-(6-((5aR，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺，N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((5aS，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((5aS，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺，N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide,

N-(6-((5aS，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺，N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide,

N-(6-((5aS，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺，N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide,

N-(6-((5aS，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-(氟甲氧基)吡啶酰胺，N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)picolinamide,

N-(6-((5aS，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺，N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide,

N-(6-((5aS，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺，N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide,

N-(6-((5aS，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺，N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((5aS，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((5aS，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺，N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide,

N-(6-((5aS，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺，N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide,

N-(6-((5aS，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺，N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide,

N-(6-((5aS，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-(氟甲氧基)吡啶酰胺，N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)picolinamide,

N-(6-((5aS，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺，N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide,

N-(6-((5aS，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺，N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide,

N-(6-((5aS，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺，N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-5-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopicinamide,

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-5-基)-5-氟吡啶-2-基)-3-氯-5-(二氟-甲氧基)吡啶酰胺，N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-(difluoro-methoxy)picolinamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-4-氯-1-(二氟甲基)-1H-吡唑-3-甲酰胺，和N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-4-chloro-1-(difluoromethyl)-1H-pyrazole-3-carboxamide, and

6-((6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)氨甲酰基)烟酸，6-((6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)carbamoyl)nicotinic acid,

或其药用盐。or a pharmaceutically acceptable salt thereof.

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

或其药用盐。or a pharmaceutically acceptable salt thereof.

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-7-氯-3-甲基咪唑并[1，2-a]吡啶-2-甲酰胺，N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-7-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-7-氯-3-甲基咪唑并[1，2-a]吡啶-2-甲酰胺，N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-7-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aR，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-7-氯-3-甲基咪唑并[1，2-a]吡啶-2-甲酰胺，N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-7-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aS，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-7-氯-3-甲基咪唑并[1，2-a]吡啶-2-甲酰胺，N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-7-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺，N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide,

N-(6-((5aR，6R，10R)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺，N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide,

N-(6-((5aR，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺，和N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide, and

N-(6-((5aS，6R，10S)-8-氨基-6，9，9-三甲基-10-氧代-3，4，5，5a，6，9-六氢-2H-[1，4]噻嗪并[2，1-g][1，2]硫杂氮杂-6-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiazepin-6-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide

或其药用盐。or a pharmaceutically acceptable salt thereof.

本发明的一个特定实施方案提供通过本文所述的方法制备的如本文所述的式I化合物。A particular embodiment of the present invention provides a compound of formula I as described herein, prepared by a process described herein.

本发明的一个特定实施方案提供如本文所述的式I化合物，其用作治疗活性物质。A certain embodiment of the invention provides compounds of formula I as described herein for use as therapeutically active substances.

本发明的一个特定实施方案提供如本文所述的式I化合物，其用作BACE1活性的抑制剂。A certain embodiment of the invention provides compounds of formula I as described herein for use as inhibitors of BACE1 activity.

本发明的一个特定实施方案提供如本文所述的式I化合物，其用作治疗活性物质，所述治疗活性物质用于治疗性和/或预防性治疗以升高的β-淀粉样蛋白水平(elevated β-amyloid levels)和/或β-淀粉样蛋白低聚物(β-amyloid oligomers)和/或β-淀粉样蛋白斑和进一步沉淀(β-amyloid plaques and further deposits)为特征的疾病和病症或阿尔茨海默病(Alzheimer′s disease)。A certain embodiment of the invention provides compounds of formula I as described herein for use as therapeutically active substances for the therapeutic and/or prophylactic treatment of diseases and disorders characterized by elevated β-amyloid levels and/or β-amyloid oligomers and/or β-amyloid plaques and further deposits, or Alzheimer's disease.

本发明的一个特定实施方案提供如本文所述的式I化合物，其用作治疗活性物质，所述治疗活性物质用于治疗性和/或预防性治疗阿尔茨海默病。A certain embodiment of the invention provides compounds of formula I as described herein for the use as therapeutically active substances for the therapeutic and/or prophylactic treatment of Alzheimer's disease.

本发明的一个特定实施方案提供一种药物组合物，所述药物组合物包含如本文所述的式I化合物和药用载体和/或药用辅助物质。A specific embodiment of the present invention provides a pharmaceutical composition comprising a compound of formula I as described herein and a pharmaceutically acceptable carrier and/or a pharmaceutically acceptable auxiliary substance.

本发明的一个特定实施方案提供如本文所述的式I化合物用于制备药物的用途，所述药物用于抑制BACE1活性。A particular embodiment of the invention provides the use of a compound of formula I as described herein for the preparation of a medicament for inhibiting BACE1 activity.

本发明的一个特定实施方案提供如本文所述的式I化合物用于制备药物的用途，所述药物用于治疗性和/或预防性治疗以升高的β-淀粉样蛋白水平和/或β-淀粉样蛋白低聚物和/或β-淀粉样蛋白斑和进一步沉淀为特征的疾病和病症或阿尔茨海默病。A particular embodiment of the invention provides the use of a compound of formula I as described herein for the preparation of a medicament for the therapeutic and/or prophylactic treatment of diseases and conditions characterized by elevated β-amyloid levels and/or β-amyloid oligomers and/or β-amyloid plaques and further deposits, or Alzheimer's disease.

本发明的一个特定实施方案提供如本文所述的式I化合物用于制备药物的用途，所述药物用于治疗性和/或预防性治疗阿尔茨海默病。A particular embodiment of the invention provides the use of a compound of formula I as described herein for the preparation of a medicament for the therapeutic and/or prophylactic treatment of Alzheimer's disease.

本发明的一个特定实施方案提供如本文中所述的式I化合物，所述化合物用于抑制BACE1活性。A certain embodiment of the invention provides a compound of formula I as described herein for use in inhibiting BACE1 activity.

本发明的一个特定实施方案提供如本文中所述的式I化合物，所述化合物用于治疗性和/或预防性治疗以升高的β-淀粉样蛋白水平和/或β-淀粉样蛋白低聚物和/或β-淀粉样蛋白斑和进一步沉淀为特征的疾病和病症或阿尔茨海默病。A particular embodiment of the invention provides a compound of formula I as described herein for use in the therapeutic and/or prophylactic treatment of diseases and conditions characterized by elevated β-amyloid levels and/or β-amyloid oligomers and/or β-amyloid plaques and further deposition, or Alzheimer's disease.

本发明的一个特定实施方案提供如本文中所述的式I化合物，所述化合物用于治疗性和/或预防性治疗阿尔茨海默病。A certain embodiment of the invention provides a compound of formula I as described herein for use in the therapeutic and/or prophylactic treatment of Alzheimer's disease.

本发明的一个特定实施方案提供一种用于抑制BACE1活性，特别是用于治疗性和/或预防性治疗以升高的β-淀粉样蛋白水平和/或β-淀粉样蛋白低聚物和/或β-淀粉样蛋白斑和进一步沉淀为特征的疾病和病症或阿尔茨海默病的方法，所述方法包括向人类或动物给药如本文所述的式I化合物。A particular embodiment of the invention provides a method for inhibiting BACE1 activity, in particular for the therapeutic and/or prophylactic treatment of diseases and disorders characterized by elevated β-amyloid levels and/or β-amyloid oligomers and/or β-amyloid plaques and further deposition, or Alzheimer's disease, comprising administering to a human or animal a compound of formula I as described herein.

本发明的一个特定实施方案提供一种用于治疗性和/或预防性治疗阿尔茨海默病的方法，所述方法包括向人类或动物给药如本文所述的式I化合物。A particular embodiment of the present invention provides a method for the therapeutic and/or prophylactic treatment of Alzheimer's disease, which method comprises administering to a human or animal a compound of formula I as described herein.

此外，本发明包括式I化合物的所有旋光异构体，即非对映异构体、非对映异构体混合物、外消旋混合物、所有它们相应的对映体和/或互变异构体以及它们的溶剂化物。Furthermore, the present invention includes all optical isomers of the compounds of formula I, ie diastereomers, diastereomeric mixtures, racemic mixtures, all their corresponding enantiomers and/or tautomers and their solvates.

本领域技术人员将认识到式I化合物可以以互变异构形式存在Those skilled in the art will recognize that compounds of Formula I may exist in tautomeric forms

所有互变异构形式都被涵盖在本发明中。All tautomeric forms are encompassed by the present invention.

式I化合物可以含有一个以上不对称中心，并且因此可以作为以下形式存在：外消旋物、外消旋混合物、单一对映体、非对映异构体混合物和单独的非对映异构体。取决于分子上不同取代基的性质，可以存在另外的不对称中心。每个这种不对称中心将独立地产生两个光学异构体，并且意图是以混合物形式的、作为纯的或部分提纯的化合物的所有可能的光学异构体和非对映异构体均包含在本发明的范围内。本发明意欲涵盖这些化合物的所有这种同分异构形式。可以如本领域中所公知的通过本文所公开的方法的适当修改形式实现这些非对映体的独立合成或它们的层析分离。可以通过结晶产物或结晶中间体的x射线晶体学确定它们的绝对立体化学，如果需要，将其用含有已知绝对构型的不对称中心的试剂衍生化。如果需要，可以将该化合物的外消旋混合物分离以将单独的对映体分离。可以通过本领域已知的方法进行分离，如化合物的外消旋混合物与对映体纯化合物的偶联以形成非对映异构体混合物，之后通过标准方法分离单独的非对映异构体，如分级结晶或色谱。Formula I compounds may contain more than one asymmetric center and therefore may exist as racemates, racemic mixtures, single enantiomers, diastereomeric mixtures, and individual diastereomers. Depending on the nature of the different substituents on the molecule, additional asymmetric centers may exist. Each such asymmetric center will independently produce two optical isomers, and it is intended that all possible optical isomers and diastereomers of a pure or partially purified compound in the form of a mixture are included within the scope of the present invention. The present invention is intended to encompass all such isomeric forms of these compounds. The independent synthesis of these diastereomers or their chromatographic separations can be achieved as known in the art by appropriate modifications of the methods disclosed herein. Their absolute stereochemistry can be determined by x-ray crystallography of the crystalline product or crystalline intermediate, and if desired, it can be derivatized with a reagent containing an asymmetric center of known absolute configuration. If desired, the racemic mixture of the compound can be separated to separate the individual enantiomers. Separation can be achieved by methods known in the art, such as coupling of a racemic mixture of the compounds with an enantiomerically pure compound to form a diastereomeric mixture, followed by separation of the individual diastereomers by standard methods, such as fractional crystallization or chromatography.

某些实施方案是以下具体形式：Certain embodiments are in the form of:

在实施方案中，在提供旋光纯对映异构体的情况下，旋光纯对映异构体意指化合物含有＞90重量％的所需异构体，特别是＞95重量％的所需异构体，或者更特别是＞99重量％的所需异构体，所述重量百分比基于化合物的一种或多种异构体的总重量。可以通过手性选择合成或通过对映体的分离制备手性纯或手性富集的化合物。可以对最终产物或者备选地对合适的中间体进行对映体的分离。In embodiments, where an optically pure enantiomer is provided, an optically pure enantiomer means that the compound contains >90% by weight of the desired isomer, particularly >95% by weight of the desired isomer, or more particularly >99% by weight of the desired isomer, the weight percentages being based on the total weight of the one or more isomers of the compound. Chirally pure or chirally enriched compounds can be prepared by chiral selective synthesis or by separation of enantiomers. Separation of enantiomers can be performed on the final product or, alternatively, on a suitable intermediate.

式I化合物可以通过许多合成路线例如方案1-14中所示制备。本发明式I化合物的制备可以通过顺次或会聚的合成路线进行。本发明化合物的合成示于以下方案中。进行所述反应和所得产物的纯化所需的技能是本领域技术人员已知的。在下面对方法的描述中使用的取代基和标志具有前文所给出的含义，除非有相反指示。Compounds of formula I can be prepared by a number of synthetic routes, such as those shown in Schemes 1-14. The preparation of compounds of formula I of the present invention can be carried out by sequential or convergent synthetic routes. The synthesis of compounds of the present invention is shown in the following schemes. The skills required to carry out the reactions and purification of the resulting products are known to those skilled in the art. Substituents and symbols used in the following description of the methods have the meanings given above, unless otherwise indicated.

关键中间体A6可以经由方案1中描述的中间体制备。可商购的2-(甲硫基)乙腈(A1)可以使用碱，如例如氢化钠，在适当的烷基化剂，例如烷基碘或烷基溴的存在下，在合适的非质子溶剂，例如四氢呋喃中烷基化，得到相应中间体A2。备选地，烷基化剂可以是适当的醛，例如多聚甲醛。之后可以通过本领域中已知的方法，例如使用合适的氟化剂，如二乙基氨基三氟化硫，将得到的羟基化合物转变为相应的卤素化合物，获得中间体A2。烷基化可以逐步进行，或，如果R¹＝R²，使用适当的试剂过量地一锅进行。Key intermediate A6 can be prepared via the intermediates described in Scheme 1. Commercially available 2-(methylthio)acetonitrile (A1) can be alkylated using a base, such as sodium hydride, in the presence of an appropriate alkylating agent, such as an alkyl iodide or alkyl bromide, in a suitable aprotic solvent, such as tetrahydrofuran, to afford the corresponding intermediate A2. Alternatively, the alkylating agent can be an appropriate aldehyde, such as paraformaldehyde. The resulting hydroxy compound can then be converted to the corresponding halogen compound by methods known in the art, such as using a suitable fluorinating agent, such as diethylaminosulfur trifluoride, to afford intermediate A2. The alkylation can be performed stepwise or, if R ¹ = R ² , in one pot using an excess of the appropriate reagent.

方案1Solution 1

可以使用本领域中已知的合适的氧化程序，例如使用过碘酸钠，间氯过氧苯甲酸或过硫酸氢钾(oxone)，将中间体A2氧化为相应亚砜A3。以下形成磺基肟部分以获得中间体A4可以通过本领域中已知的方法实现，例如在两个步骤中使用例如催化量的四乙酸二铑，和化学计量量的二乙酰氧基亚碘酰苯，三氟乙酰胺和氧化镁，接着例如使用碳酸钾在低级醇中水解，或，备选地，使用催化量的4，4’，4”-三叔丁基-2，2’：6’，2”-三吡啶和硝酸银，和化学计量量的4-硝基苯磺酰胺和二乙酰氧基亚碘酰苯，并且随后使用硫代苯酚和碳酸铯水解，两个步骤在适当的溶剂中。备选地，中间体A4可以在一个步骤中使用化学计量量的叠氮化钠在Eaton试剂(即，五氧化二磷在甲磺酸中的溶液)中合成。中间体A4可以随后与适当的烷基化试剂A5，在合适的碱，例如氢化钠，氢化钾或碳酸铯，和任选地催化量的季铵盐，例如四正丁基溴化铵或四正丁基氯化铵的存在下，在合适的非质子溶剂，例如二甲氧基乙烷，四氢呋喃或乙腈中反应，得到中间体A6。烷基化试剂A5是保护的卤代-醇，其中X意为离去基团，例如卤素，(取代的)芳烃-或(取代的)烷烃磺酸酯基，优选溴，碘或三氟甲磺酸酯基，并且PG意为保护基团，例如四氢吡喃基。Intermediate A2 can be oxidized to the corresponding sulfoxide A3 using suitable oxidation procedures known in the art, such as sodium periodate, m-chloroperbenzoic acid or potassium persulfate (oxone). The subsequent formation of the sulfoxime moiety to obtain intermediate A4 can be achieved by methods known in the art, such as using, for example, a catalytic amount of dirhodium tetraacetate and a stoichiometric amount of diacetoxyiodobenzophenone, trifluoroacetamide and magnesium oxide in two steps, followed by hydrolysis in a lower alcohol, such as using potassium carbonate, or, alternatively, using a catalytic amount of 4,4',4"-tri-tert-butyl-2,2':6',2"-tripyridine and silver nitrate, and a stoichiometric amount of 4-nitrobenzenesulfonamide and diacetoxyiodobenzophenone, and then hydrolyzing using thiophenol and cesium carbonate, two steps in an appropriate solvent. Alternatively, intermediate A4 can be synthesized in one step using a stoichiometric amount of sodium azide in Eaton's reagent (i.e., a solution of phosphorus pentoxide in methanesulfonic acid). Intermediate A4 can then be reacted with an appropriate alkylating agent A5 in the presence of a suitable base, such as sodium hydride, potassium hydride or cesium carbonate, and optionally a catalytic amount of a quaternary ammonium salt, such as tetra-n-butylammonium bromide or tetra-n-butylammonium chloride, in a suitable aprotic solvent, such as dimethoxyethane, tetrahydrofuran or acetonitrile, to give intermediate A6. Alkylating agent A5 is a protected halo-alcohol, wherein X is a leaving group, such as halogen, (substituted) arene- or (substituted) alkanesulfonate, preferably bromine, iodine or triflate, and PG is a protecting group, such as tetrahydropyranyl.

方案2Option 2

关键中间体A6可以随后与磺基肟A7在强碱，例如六甲基二硅基氨基碱，如六甲基二硅基氨基锂，二异丙基氨基碱，如二异丙基氨基锂，或烷基锂，如正丁基锂的存在下，在无水条件下在合适的非质子溶剂，例如四氢呋喃或二氯甲烷中反应，形成中间体混合物-A8，为立体异构体的混合物(方案2)。在该阶段可以通过色谱和方案2中描述的途径分离单个立体异构体，并且下列方案可以类似使用分离的单个异构体接着。备选地，可以将立体异构体的混合物脱保护并且可以断开磺酰胺部分，得到相应氨基醇A9，为对映异构富集的非对映异构体S_R-A9和S_S-A9的混合物。前缀S_X表示硫原子处的绝对构型(分别地，对于(S)为S_S-和对于(R)为S_R-)。在中间体混合物-A8中的保护基团PG是酸不稳定的情况下，例如是四氢吡喃基的情况下，上述两种断开可以在酸性条件下，例如使用氯化氢在醇，如甲醇或乙醇中的溶液，以一个步骤进行。两种对映体富集的非对映异构体S_R-A9和S_S-A9可以通过色谱或通过本领域中已知的其他方式分离。备选地，非对映异构体的混合物可以通过合成得到，并且各自得到的混合物可以在稍后的阶段通过色谱或通过本领域中已知的其他方式分离。The key intermediate A6 can then be reacted with a sulfoxime A7 in the presence of a strong base, such as hexamethyldisilazide, such as lithium hexamethyldisilazide, diisopropylamide, such as lithium diisopropylamide, or an alkyllithium, such as n-butyllithium, under anhydrous conditions in a suitable aprotic solvent, such as tetrahydrofuran or dichloromethane, to form an intermediate mixture-A8, which is a mixture of stereoisomers (Scheme 2). At this stage, the individual stereoisomers can be separated by chromatography and the approach described in Scheme 2, and the following scheme can be followed by similar use of the isolated individual isomers. Alternatively, the mixture of stereoisomers can be deprotected and the sulfonamide moiety can be disconnected to obtain the corresponding amino alcohol A9, which is a mixture of enantiomerically enriched diastereomers _SR -A9 and _SS -A9. The prefix _SX represents the absolute configuration at the sulfur atom (respectively, _SS- for (S) and _SR- for (R)). In the case where the protecting group PG in the intermediate compound-A8 is acid-labile, for example, a tetrahydropyranyl group, the two cleavages described above can be performed in a single step under acidic conditions, for example, using a solution of hydrogen chloride in an alcohol such as methanol or ethanol. The two enantiomerically enriched diastereomers, _SR -A9 and _SS -A9, can be separated by chromatography or other means known in the art. Alternatively, a mixture of diastereomers can be obtained by synthesis, and the resulting mixtures can be separated at a later stage by chromatography or other means known in the art.

方案3Option 3

接着，中间体S_R-A9可以使用本领域中已知的方法，例如使用化学计量量的铜(I)盐，例如氯化铜(I)或溴化铜(I)，在合适的溶剂，例如醇，如乙醇中，在升高的温度，如20℃至130℃，优选在70℃至90℃，环化为中间体S_R-A10(方案3)。备选地，该转变可以使用化学计量量的路易斯酸，如三甲基铝，在合适的非质子溶剂，如甲苯中实现。随后通过适当的保护基团PG保护中间体S_R-A10中的脒官能团，得到中间体S_R-A11。保护基团PG应该对碱性条件稳定并且可以是，例如叔丁氧基羰基(BOC)。在PG是BOC的情况下，向中间体S_R-A11的转变可以如下实现：使用本领域中已知的条件，例如二碳酸二叔丁酯，在合适的碱，如碳酸氢钠的存在下，并且任选地在催化量的合适的路易斯碱，例如4-(二甲基氨基)-吡啶的存在下，接着加入合适的亲核试剂，例如氨水，以消出过量的二碳酸二叔丁酯，之后浓缩反应混合物。之后，将中间体S_R-A11中的羟基转变为中间体S_R-A12中的离去基团OLG。合适的离去基团包括芳烃磺酰基(sulfonoyl)，例如对甲苯磺酰基，烷烃磺酰基，例如三氟甲烷磺酰基，或卤素，例如碘。如果OLG是对甲苯磺酰基，至中间体S_R-A12的转变可以在本领域中已知的标准条件下，使用例如，对甲苯磺酰氯，在合适的碱，例如叔胺，如三乙胺或二异丙基乙胺，和，任选地催化量的合适的路易斯碱，例如4-(二甲基氨基)-吡啶的存在下实现。如果OLG是碘化物，至中间体S_R-A12的转变可以在本领域中已知的标准条件下，使用例如四烷基碘化铵，例如四正丁基碘化铵，在合适的膦，例如三苯基膦，和合适的活化剂，如2，3-二氯-5，6-二氰基-1，4-苯醌(DDQ)的存在下，在非质子溶剂，例如二氯甲烷中实现。Next, intermediate _SR -A9 can be cyclized to intermediate SR-A10 using methods known in the art, for example, using a stoichiometric amount of a copper(I) salt, such as copper(I) chloride or copper(I) bromide, in a suitable solvent, for example, an alcohol, such as ethanol, at elevated temperatures, such as 20°C to 130°C, preferably 70°C to 90° _C (Scheme 3). Alternatively, this transformation can be achieved using a stoichiometric amount of a Lewis acid, such as trimethylaluminum, in a suitable aprotic solvent, such as toluene. The amidine function in intermediate _SR -A10 is then protected by an appropriate protecting group PG to provide intermediate _SR -A11. The protecting group PG should be stable to basic conditions and can be, for example, tert-butoxycarbonyl (BOC). In the case where PG is BOC, conversion to the intermediate _SR -A11 can be achieved using conditions known in the art, such as di-tert-butyl dicarbonate in the presence of a suitable base, such as sodium bicarbonate, and optionally in the presence of a catalytic amount of a suitable Lewis base, such as 4-(dimethylamino)-pyridine, followed by addition of a suitable nucleophile, such as ammonia, to eliminate excess di-tert-butyl dicarbonate, followed by concentration of the reaction mixture. The hydroxyl group in the intermediate _SR -A11 is then converted to a leaving group, OLG, in the intermediate _SR -A12. Suitable leaving groups include arenesulfonyl, such as p-toluenesulfonyl, alkanesulfonyl, such as trifluoromethanesulfonyl, or halogen, such as iodine. If OLG is p-toluenesulfonyl, the conversion to the intermediate _SR -A12 can be achieved under standard conditions known in the art using, for example, p-toluenesulfonyl chloride in the presence of a suitable base, for example, a tertiary amine such as triethylamine or diisopropylethylamine, and, optionally, a catalytic amount of a suitable Lewis base, for example, 4-(dimethylamino)-pyridine. If OLG is an iodide, the conversion to the intermediate _SR -A12 can be achieved under standard conditions known in the art using, for example, tetraalkylammonium iodides, for example, tetra-n-butylammonium iodide, in the presence of a suitable phosphine, for example, triphenylphosphine, and a suitable activating agent, such as 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), in an aprotic solvent, for example, dichloromethane.

类似于以上和在方案3中对于中间体S_R-A9至中间体S_R-A12的转变所述的化学，中间体S_S-A9可以转变为中间体S_S-A12(方案4)。Analogous to the chemistry described above and in Scheme 3 for the conversion of intermediate _SR -A9 to intermediate _SR -A12, intermediate _SS -A9 can be converted to intermediate _SS -A12 (Scheme 4).

方案4Option 4

以下从中间体S_R-A12开始的环化步骤得到两种非对映异构体S_R-A13和S_R-A14(方案5)。两种非对映异构体可以通过色谱的方式或本领域中已知的其他方式分离，或混合物可以在接着的步骤中反应并且反应产物在后来的阶段通过色谱的方式或本领域中已知的其他方式分离。备选地，非对映异构体S_R-A13和S_R-A14的混合物可以在接下来的步骤中利用反应性差异反应。同样，如果两种非对映异构体S_R-A13或S_R-A14中的一种在下一步骤中具有更高的反应性，该差异可以用于通过色谱方法分离两种非对映异构体。转变可以使用强碱，如，例如，六甲基二硅基氨基锂或异丙基氨基锂，在无水条件下，在非质子溶剂，例如四氢呋喃中，在-80℃至0℃的温度实现。在某些情况下，由于空间位阻或热力学原因，两种可能的非对映异构体中的一种是强烈偏爱的。The following cyclization step starting from the intermediate _SR -A12 gives two diastereomers, _SR -A13 and _SR -A14 (Scheme 5). The two diastereomers can be separated by chromatography or other means known in the art, or the mixture can be reacted in a subsequent step and the reaction products separated at a later stage by chromatography or other means known in the art. Alternatively, the mixture of diastereomers _SR -A13 and _SR -A14 can be reacted in a subsequent step to exploit the difference in reactivity. Similarly, if one of the two diastereomers _SR -A13 or _SR -A14 has a higher reactivity in the next step, this difference can be used to separate the two diastereomers by chromatography. The transformation can be achieved using a strong base, such as, for example, lithium hexamethyldisilazide or lithium isopropylamide, under anhydrous conditions in an aprotic solvent, such as tetrahydrofuran, at a temperature between -80°C and 0°C. In certain cases, due to steric hindrance or thermodynamic reasons, one of the two possible diastereomers is strongly favored.

方案5Option 5

类似于以上和方案5中对于中间体S_R-A12向中间体S_R-A13和S_R-A14转变所描述的化学，中间体S_S-A12可以转变为中间体S_S-A13和S_S-A14(方案6)。Analogous to the chemistry described above and in Scheme 5 for the conversion of intermediate _SR -A12 to intermediates _SR -A13 and _SR -A14, intermediate _SS -A12 can be converted to intermediates _SS -A13 and _SS -A14 (Scheme 6).

方案6Option 6

接着，使用本领域中已知的方法，例如使用氟化物盐，如氟化钾，在合适的酸，例如乙酸的存在下，在极性溶剂，如四氢呋喃和二甲基甲酰胺中，或使用四烷基氟化铵，例如四正丁基氟化铵，在适当的极性溶剂，如四氢呋喃中，断开中间体S_R-A13中的甲硅烷基，得到中间体S_R-A15(方案7)。接着，使用过量的叠氮化钠，催化或化学计量量的合适的铜(I)盐，如碘化铜(I)，和合适的二氨基配体，如反式-N，N’-二甲基环已烷-1，2-二胺，和亚化学计量量的抗坏血酸钠，在适当的极性溶剂，如二烷和水中，在升高的温度，例如60℃至80℃，将中间体S_R-A15转变为中间体S_R-A16。Next, the silyl group in intermediate SR-A13 is cleaved using methods known in the art, for example, using a fluoride salt, such as potassium fluoride, in the presence of a suitable acid, such as acetic acid, in a polar solvent, such as tetrahydrofuran and dimethylformamide, or using a tetraalkylammonium fluoride, such as tetra-n-butylammonium fluoride, in a suitable polar solvent, such as tetrahydrofuran, to provide intermediate _SR -A15 (Scheme 7). Intermediate _SR -A15 is then converted to intermediate SR-A16 using an excess of sodium azide, a catalytic or stoichiometric amount of a suitable copper(I) salt, such as copper(I) iodide, and a suitable diamino ligand, such as trans-N,N'-dimethylcyclohexane-1,2-diamine, and a substoichiometric amount of sodium ascorbate in a suitable polar solvent, such as dioxane and water, at an elevated temperature, for example, 60° _C to 80° _C .

方案7Option 7

类似于以上和方案7中对于中间体S_R-A13向中间体S_R-A16的转变所述的化学，中间体S_S-A13可以转变为中间体S_S-A16(方案8)。Analogous to the chemistry described above and in Scheme 7 for the conversion of intermediate _SR -A13 to intermediate _SR -A16, intermediate _SS -A13 can be converted to intermediate _SS -A16 (Scheme 8).

方案8Option 8

类似于以上和方案7中对于中间体S_R-A13向中间体S_R-A16的转变所述的化学，中间体S_R-A14可以转变为中间体S_R-A18(方案9)。Analogous to the chemistry described above and in Scheme 7 for the conversion of intermediate _SR -A13 to intermediate _SR -A16, intermediate _SR -A14 can be converted to intermediate _SR -A18 (Scheme 9).

方案9Option 9

类似于以上和方案7中对于中间体S_R-A13向中间体S_R-A16的转变所述的化学，中间体S_S-A14可以转变为中间体S_S-A18(方案10)。Analogous to the chemistry described above and in Scheme 7 for the conversion of intermediate _SR -A13 to intermediate _SR -A16, intermediate _SS -A14 can be converted to intermediate _SS -A18 (Scheme 10).

方案10Plan 10

之后，通过本领域中已知的合适的酰胺键形成方法，使用适当的酸R⁸COOH，其中R⁸如上文定义，酰化中间体S_R-A16以形成中间体S_R-A19(方案11)。作为实例，这些方法包括，中间体S_R-A16与酸R⁸COOH在化学计量量的1-氯-N，N，2-三甲基丙烯基胺和合适的碱，例如叔胺，如二异丙基乙胺的存在下，在非质子溶剂，例如二氯甲烷中，在-10℃至30℃的温度的反应。备选地，可以使用本领域中已知的方法，例如使用草酰氯或亚硫酰氯，在非质子溶剂，如二氯甲烷或甲苯中将酸R⁸COOH转变为相应的酰氯R⁸COCl。分离的酰氯R⁸COCl可以随后与中间体S_R-A16在合适的碱，例如叔胺，如二异丙基乙胺的存在下，在非质子溶剂，例如二氯甲烷中，在-10℃至30℃的温度反应，形成中间体S_R-A19。最后，通过本领域中已知的方法将中间体S_R-A19脱保护，得到最终化合物S_R-A20。如果PG是BOC，脱保护在过量强酸，如三氟乙酸或氯化氢的存在下，在合适的溶剂，如二氯甲烷或四氢呋喃中或没有溶剂的情况下，在净(neat)条件下(如果可行)搅拌中间体S_R-A19实现。接着，通过色谱，使用合适的手性固定相将对映异构富集的式S_R-A20的产物纯化为它们的对映异构纯形式。The intermediate _SR -A16 is then acylated by suitable amide bond forming methods known in the art using an appropriate acid R ⁸ COOH, wherein R ⁸ is as defined above, to form the intermediate _SR -A19 (Scheme 11). As an example, these methods include the reaction of the intermediate _SR -A16 with an acid R ⁸ COOH in the presence of a stoichiometric amount of 1-chloro-N,N,2-trimethylpropenylamine and a suitable base, for example a tertiary amine such as diisopropylethylamine, in an aprotic solvent such as dichloromethane at a temperature of -10°C to 30°C. Alternatively, the acid R 8 COOH can be converted to the corresponding acid chloride R ⁸ COCl using methods known in the art, for example using oxalyl chloride or thionyl chloride in an aprotic solvent such as ^{dichloromethane} or toluene. The isolated acid chloride ^R8COCl can then be reacted with the intermediate _SR -A16 in the presence of a suitable base, for example a tertiary amine such as diisopropylethylamine, in an aprotic solvent such as dichloromethane at a temperature between -10°C and 30°C to form the intermediate _SR -A19. Finally, the intermediate _SR -A19 is deprotected by methods known in the art to afford the final compound _SR -A20. If PG is BOC, deprotection is achieved by stirring the intermediate SR-A19 under neat conditions (if applicable) in the presence of an excess of a strong acid such as trifluoroacetic acid or hydrogen chloride in a suitable solvent such as dichloromethane or tetrahydrofuran or without a solvent. The enantiomerically enriched products of formula _SR _- A20 are then purified to their enantiomerically pure form by chromatography using a suitable chiral stationary phase.

类似于以上和方案11中对于中间体S_R-A16向对映异构纯化的最终化合物S_R-A20的转变所描述的化学，可以将中间体S_S-A16转变为对映异构纯化的最终化合物S_S-A20(方案12)。Intermediate _SS -A16 can be converted to enantiomerically purified final compound _SS -A20 (Scheme 12) analogous to the chemistry described above and in Scheme 11 for the transformation of intermediate _SR -A16 to enantiomerically purified final compound _SR -A20.

方案12Plan 12

类似于以上和方案11中对于中间体S_R-A16向对映异构纯化的最终化合物S_R-A20的转变所述的化学，可以将中间体S_R-A18转变为对映异构纯化的最终化合物S_R-A22(方案13)。Intermediate _SR -A18 can be converted to enantiomerically purified final compound _SR -A22 (Scheme 13) similar to the chemistry described above and in Scheme 11 for the conversion of intermediate _SR -A16 to enantiomerically purified final compound _SR -A20.

方案13Plan 13

类似于以上和方案11中对于中间体S_R-A16向对映异构纯化的最终化合物S_R-A20的转变所描述的化学，可以将中间体S_S-A18转变为对映异构纯化的最终化合物S_S-A22(方案14)。Intermediate _SS -A18 can be converted to enantiomerically purified final compound _SS -A22 (Scheme 14) similar to the chemistry described above and in Scheme 11 for the conversion of intermediate _SR -A16 to enantiomerically purified final compound _SR -A20.

方案14Plan 14

方案7和11中所述的为了获得最终式S_R-A20的化合物的合成步骤的顺序可以适当改变。类似地，方案7中的脱硅(desilation)步骤可以例如在方案11中的顺序的最后进行。The order of the synthetic steps described in Schemes 7 and 11 to obtain the final compound of formula _SR -A20 can be changed appropriately. Similarly, the desilation step in Scheme 7 can be performed, for example, at the end of the sequence in Scheme 11.

方案8和12中所述的为了获得最终式S_S-A20的化合物的合成步骤的顺序可以适当改变。类似地，方案8中的脱硅步骤可以例如在方案12中的顺序的最后进行。The order of the synthetic steps described in Schemes 8 and 12 to obtain the final compound of formula _SS -A20 can be changed appropriately. Similarly, the desilylation step in Scheme 8 can be performed, for example, at the end of the sequence in Scheme 12.

方案9和13中所述的为了获得最终式S_R-A22的化合物的合成步骤的顺序可以适当改变。类似地，方案9中的脱硅步骤可以例如在方案13中的顺序的最后进行。The order of the synthetic steps described in Schemes 9 and 13 to obtain the final compound of formula _SR -A22 can be changed appropriately. Similarly, the desilylation step in Scheme 9 can be performed, for example, at the end of the sequence in Scheme 13.

方案10和14中所述的为了获得最终式S_S-A22的化合物的合成步骤的顺序可以适当改变。类似地，方案10中的脱硅步骤可以例如在方案14中的顺序的最后进行。The order of the synthetic steps described in Schemes 10 and 14 to obtain the final compound of formula _SS -A22 can be changed appropriately. Similarly, the desilylation step in Scheme 10 can be performed, for example, at the end of the sequence in Scheme 14.

可以通过本领域技术人员已知的标准方法获得相应的与酸的药用盐，例如通过将式I化合物溶解在合适的溶剂如二烷或四氢呋喃中并加入合适量的相应的酸获得。通常可以通过过滤或通过色谱分离产物。用碱将式I化合物转化为药用盐可以通过将这种化合物用这样的碱处理而进行。形成这种盐的一种可能方法是例如通过向该化合物在合适的溶剂(例如乙醇、乙醇-水混合物、四氢呋喃-水混合物)中的溶液中，加入1/n当量的碱盐如M(OH)_n，其中M＝金属或铵阳离子，并且n＝氢氧根阴离子的数量，并且通过蒸发或冷冻干燥移除溶剂。特别的盐是盐酸盐、甲酸盐和三氟乙酸盐。The corresponding pharmaceutical salts with acids can be obtained by standard methods known to those skilled in the art, for example, by dissolving the compound of formula I in a suitable solvent such as dioxane or tetrahydrofuran and adding a suitable amount of the corresponding acid. The product can usually be separated by filtration or by chromatography. The conversion of the compound of formula I into a pharmaceutical salt with a base can be carried out by treating the compound with such a base. A possible method of forming such a salt is, for example, by adding 1/n equivalents of an alkali salt such as M(OH) _n to a solution of the compound in a suitable solvent (e.g., ethanol, ethanol-water mixture, tetrahydrofuran-water mixture), wherein M=metal or ammonium cation, and n=the number of hydroxide anions, and removing the solvent by evaporation or freeze drying. Particular salts are hydrochloride, formate and trifluoroacetate.

在其制备未被描述在实施例中的情况下，可以根据类似方法或根据在此给出的方法制备式I化合物以及所有中间体产物。原材料是可商购的，本领域已知的或者可以通过本领域已知方法或与其类似的方法制备。Where their preparation is not described in the examples, the compounds of formula I and all intermediate products can be prepared by analogous methods or by the methods given herein. The starting materials are commercially available, known in the art or can be prepared by methods known in the art or by methods analogous thereto.

应理解，可以将本发明中通式I化合物在官能团处进行衍生以提供能够在体内转化回母体化合物的衍生物。It will be appreciated that the compounds of general formula I in this invention may be derivatized at functional groups to provide derivatives which are capable of conversion back to the parent compound in vivo.

药理学测试Pharmacological testing

式I化合物和它们的药用盐拥有有价值的药理学特性。已经发现本发明的化合物与BACEl活性的抑制有关。按照在下文中给出的测试研究该化合物。The compounds of formula I and their pharmaceutically acceptable salts possess valuable pharmacological properties. It has been found that the compounds of the invention are involved in the inhibition of BACE1 activity. The compounds were studied in the tests given below.

细胞Aβ-降低测定：Cellular Aβ-lowering assay:

可以使用Aβ40AlphaLISA测定。将HEK293APP细胞接种在96孔微量滴定板中的细胞培养基(Iscove’s，加10％(v/v)胎牛血清、青霉素/链霉素)中，至约80％会合并且将化合物以在1/3体积培养基中的3x浓度加入(保持终DMSO浓度为1％v/v)。在加湿培养箱中在37℃和5％CO₂下孵育18-20hrs后，收获培养上清，用于使用Perkin-Elmer人淀粉样蛋白β1-40(高特异性)试剂盒(Cat#AL275C)确定Aβ40浓度。Aβ40AlphaLISA can be used to determine. HEK293APP cells are seeded in cell culture medium (Iscove's, plus 10% (v/v) fetal bovine serum, penicillin/streptomycin) in 96-well microtiter plates to about 80% confluence and the compound is added at a 3x concentration in 1/3 volume culture medium (keeping the final DMSO concentration at 1% v/v). After incubation for 18-20 hrs at 37°C and 5% _CO2 in a humidified incubator, the culture supernatant is harvested and used to determine Aβ40 concentration using the Perkin-Elmer Human Amyloid β1-40 (High Specificity) Kit (Cat#AL275C).

在Perkin-Elmer White Optiplate-384(Cat#6007290)中，将2ul培养上清与2μl10X AlphaLISA抗-hAβ受体珠+生物素化抗体抗-Aβ1-40 Mix(50μg/mL/5nM)合并。室温孵育1小时后，将16μl的1.25X链霉亲和素(SA)供体珠制备物(25μg/mL)加入并且在暗处孵育30分钟。然后使用EnVision-Alpha读数器记录在615nm处的光发射。将培养上清中Aβ40水平计算为最大信号(用1％DMSO而不用抑制剂处理的细胞)的百分比。使用Excel XLfit软件计算IC₅₀值。In Perkin-Elmer White Optiplate-384 (Cat#6007290), 2ul of culture supernatant was combined with 2μl of 10X AlphaLISA anti-hAβ acceptor beads + biotinylated antibody anti-Aβ1-40 Mix (50μg/mL/5nM). After incubation at room temperature for 1 hour, 16μl of 1.25X streptavidin (SA) donor bead preparation (25μg/mL) was added and incubated in the dark for 30 minutes. The light emission at 615nm was then recorded using an EnVision-Alpha reader. The Aβ40 level in the culture supernatant was calculated as a percentage of the maximum signal (cells treated with 1% DMSO but not with inhibitors). _IC50 values were calculated using Excel XLfit software.

降低野生型小鼠脑中的Aβ40：Reduction of Aβ40 in the brains of wild-type mice:

动物和安置条件.将动物维持在12/12h明/暗循环，明亮在上午6点开始，并且在明亮期期间进行实验。动物安置和实验步骤符合伦理和法律准则并且得到当地兽医当局批准。Animals and Housing Conditions. Animals were maintained on a 12/12 h light/dark cycle with light onset at 6:00 AM, and experiments were performed during the light period. Animal housing and experimental procedures complied with ethical and legal guidelines and were approved by local veterinary authorities.

实验.将雌性C57B1/6J小鼠用30mg/kg化合物的剂量处理，每个治疗组3-4只动物。将测试化合物溶解在5％EtOH，10％Solutol中，并且以10mL/kg口服给药。4h后，处死动物并且收集脑和血浆。将脑切成两半并且立即在干冰上冷冻。将脑用于测量Aβ40并且血浆用于确定化合物暴露。用于脑裂解物中Aβ40确定的方法按照已知步骤(Lanz，T.A.；Schachter，J.B.Demonstration of a common artifact in immunosorbent assays of brainextracts：development of a solid phase extraction protocol to enablemeasurement of amyloid-βfrom wild-type rodent brain.J.Neurosci.Methods2006，157，71-81.)。将脑组织在2％DEA缓冲液中在Roche MagnaLyser(20″，4000rpm)中匀浆，并且接着在100000g离心1h。将DEA降低至在50mM NaCl中0.2％并且将一半DEA裂解物通过Oasis固相提取板(Waters；目录号186000679)，该Oasis固相提取板已经用MeOH活化并且在dH2O(每个1mL)中平衡。在10％和30％MeOH(每个1mL)中洗涤后，将Aβ-肽在0.8mL在90％MeOH中的2％NH4OH中洗脱。将洗脱物经N2流干燥，并且将干燥的样品在30μL的AlphaLISA测定缓冲液中恢复。通过AlphaLISA测定(Perkin-Elmer)确定Aβ40。在白色96孔，半区域微孔板(Perkin-Elmer目录号6005561)中，将20μL恢复的样品与5μL生物素化的BAP-24(对Aβ40的C末端特异)混合(Brockhaus，M.；Grunberg，J.；Rohrig，S.；Loetscher，H.；Wittenburg，N.；Baumeister，R.；Jacobsen，H.；Haass，C.Caspasemediated cleavage is not requiredfor the activity of presenilins in amyloidogenesis and NOTCHsignaling.NeuroReport 1998，9，1481-1486.)储液＝4.4mg/mL，f.c.5.5μg/mL)，并且5μL252Q6受体珠(252Q6抗体，Invitrogen AMB0062)之前已经与AlphaLISA受体珠(Perkin-Elmer目录号6772002)缀合；最终稀释度1∶500)。将混合物在室温在暗处温育1h。然后加入20μL链霉亲和素包被的供体珠(Perkin-Elmer目录号6760002，最终稀释度1∶125)，并将该最终混合物在暗处在室温温育另外30min，之后在AlphaScreen读数器(Perkin-ElmerEnvision 2104)中测量RFU。在治疗的动物中对于Aβ40获得的值与媒介物组中的值相关并且以％给出。备选地，按照制造商的使用说明，商用ELISA用于Aβ40确定(Wako ELISA：(″Human/RatβAmyloid(40)ELISA kit Wako II″；目录号294-64701)。此外，在这里，Aβ-降低效率计算为媒介物组的百分数。Experimental. Female C57Bl/6J mice were treated with a dose of 30 mg/kg compound, with 3-4 animals per treatment group. The test compound was dissolved in 5% EtOH, 10% Solutol and administered orally at 10 mL/kg. After 4 h, the animals were sacrificed and the brain and plasma were collected. The brain was cut in half and immediately frozen on dry ice. The brain was used to measure Aβ40 and the plasma was used to determine compound exposure. The method for determining Aβ40 in brain lysates followed known procedures (Lanz, T.A.; Schachter, J.B. Demonstration of a common artifact in immunosorbent assays of brain extracts: development of a solid phase extraction protocol to enable measurement of amyloid-β from wild-type rodent brain. J. Neurosci. Methods 2006, 157, 71-81.). Brain tissue was homogenized in 2% DEA buffer in a Roche MagnaLyser (20", 4000 rpm) and then centrifuged at 100,000 g for 1 h. DEA was reduced to 0.2% in 50 mM NaCl and half of the DEA lysate was passed through an Oasis solid phase extraction plate (Waters; Cat. No. 186000679) that had been activated with MeOH and equilibrated in dH2O (1 mL each). After washing in 10% and 30% MeOH (1 mL each), Aβ-peptides were eluted in 0.8 mL of 2% NH4OH in 90% MeOH. The eluate was dried over a stream of N2 and the dried sample was recovered in 30 μL of AlphaLISA assay buffer. The Aβ-peptide was detected by AlphaLISA assay (Per In a white 96-well, half-area microplate (Perkin-Elmer catalog number 6005561), 20 μL of recovered sample was mixed with 5 μL of biotinylated BAP-24 (specific for the C-terminus of Aβ40) (Brockhaus, M.; Grunberg, J.; Rohrig, S.; Loetscher, H.; Wittenburg, N.; Baumeister, R.; Jacobsen, H.; Haass, C. Caspase-mediated Cleavage is not required for the activity of presenilins in amyloidogenesis and NOTCH signaling. NeuroReport 1998, 9, 1481-1486.) (stock solution = 4.4 mg / mL, f.c. 5.5 μg / mL), and 5 μL 252Q6 acceptor beads (252Q6 antibody, Invitrogen AMB0062) previously conjugated to AlphaLISA acceptor beads (Perkin-Elmer catalog number 6772002); final dilution 1: 500). The mixture was incubated at room temperature in the dark for 1 hour. Then 20 μL of streptavidin-coated donor beads (Perkin-Elmer catalog number 6760002, final dilution 1: 125) were added, and the final mixture was incubated in the dark at room temperature for another 30 minutes, after which the final mixture was read on an AlphaScreen reader (Perkin-Elmer Envision 2104). The values obtained for Aβ40 in treated animals are related to the values in the vehicle group and are given in %. Alternatively, a commercial ELISA was used for Aβ40 determination (Wako ELISA: ("Human/RatβAmyloid(40)ELISA kit Wako II"; catalog number 294-64701) according to the manufacturer's instructions. In addition, here, the Aβ-reducing efficiency is calculated as a percentage of the vehicle group.

表1：药理学数据Table 1: Pharmacological data

药物组合物Pharmaceutical composition

式I化合物和药用盐可以用作治疗活性物质，例如以药物制剂的形式。该药物制剂可以口服服用，例如，以片剂、包衣片剂、糖锭剂、硬和软明胶胶囊、溶液、乳剂或混悬剂形式。然而，也可以通过直肠实现给药，例如，以栓剂的形式，或者经肠胃外给药，例如，以针剂的形式。The compounds of formula I and pharmaceutically acceptable salts can be used as therapeutically active substances, for example in the form of pharmaceutical preparations. The pharmaceutical preparations can be taken orally, for example in the form of tablets, coated tablets, lozenges, hard and soft gelatin capsules, solutions, emulsions or suspensions. However, administration can also be achieved rectally, for example in the form of suppositories, or parenterally, for example in the form of injections.

可以将式I化合物及其药用盐与制药学惰性的、无机或有机的载体进行加工用于制备药物制剂。例如，可以使用乳糖、玉米淀粉或其衍生物、滑石、硬脂酸或其盐等作为用于片剂、包衣片剂、糖锭和硬明胶胶囊的载体。适用于软明胶胶囊的载体是例如植物油、蜡、脂肪以及半固体和液体多元醇等。然而，取决于活性成分的性质，在软明胶胶囊的情况下经常不需要载体。用于制备溶液和糖浆的适合的载体是例如水、多元醇、甘油、植物油等。适用于栓剂的载体是，例如，天然或硬化油、蜡、脂肪和半液体或液体多元醇等。Formula I compound and pharmaceutical salt thereof and pharmaceutically inert, inorganic or organic carrier can be processed for the preparation of pharmaceutical preparations.For example, lactose, corn starch or its derivatives, talcum, stearic acid or its salt etc. can be used as the carrier for tablets, coated tablets, lozenges and hard gelatin capsules.Carriers suitable for soft gelatin capsules are such as vegetable oils, waxes, fats and semisolid and liquid polyols etc. However, depending on the property of the active ingredient, carriers are often not needed in the case of soft gelatin capsules.Carriers suitable for preparing solutions and syrups are such as water, polyols, glycerol, vegetable oils etc.Carriers suitable for suppositories are, for example, natural or hardened oils, waxes, fats and semiliquid or liquid polyols etc.

此外，药物制剂可以含有药用助剂物质如防腐剂、增溶剂、稳定剂、湿润剂、乳化剂、甜味剂、着色剂、增香剂、用于改变渗透压的盐、缓冲剂、掩蔽剂或抗氧化剂。它们还可以含有其它在治疗学上有价值的物质。In addition, the pharmaceutical preparations may contain pharmaceutical auxiliary substances such as preservatives, solubilizers, stabilizers, wetting agents, emulsifiers, sweeteners, coloring agents, flavoring agents, salts for varying the osmotic pressure, buffers, masking agents or antioxidants. They may also contain other therapeutically valuable substances.

含有式I化合物或其药用盐以及治疗惰性载体的药物也可以通过本发明提供，与用于其制备的方法一样，所述方法包括将一种或多种式I化合物和/或其药用盐以及，如果需要，一种或多种其他有治疗价值的物质与一种或多种治疗惰性载体一起制成盖仑给药形式。Medicaments containing a compound of formula I or a pharmaceutically acceptable salt thereof and a therapeutically inert carrier can also be provided by the present invention, as can a process for their preparation, which comprises bringing one or more compounds of formula I and/or a pharmaceutically acceptable salt thereof and, if desired, one or more other therapeutically valuable substances into a galenic administration form together with one or more therapeutically inert carriers.

剂量可以在宽范围内变化，当然，在每个具体病例中必须根据个体需求而调节。在口服给药的情形中，用于成人的剂量可以从约0.01mg/天变化至约1000mg/天的通式I化合物或相应量的其药用盐。该日剂量可以以单一剂量给药或以分开的剂量给药，并且此外，当需要时，也可以超过该上限。The dosage can vary within a wide range and, of course, must be adjusted according to individual needs in each specific case. In the case of oral administration, the dosage for adults can vary from about 0.01 mg/day to about 1000 mg/day of a compound of formula I or a corresponding amount of a pharmaceutically acceptable salt thereof. This daily dose can be administered in a single dose or in divided doses, and, in addition, when necessary, this upper limit can also be exceeded.

以下实施例说明本发明，但不对其进行限制，而是仅作为其示例。药物制剂便利地含有约1-500mg，尤其是1-100mg的式I化合物。根据本发明的组合物的实例为：The following examples illustrate the present invention but do not limit it, but serve merely as examples thereof. Pharmaceutical preparations conveniently contain about 1-500 mg, in particular 1-100 mg, of a compound of formula I. Examples of compositions according to the invention are:

实施例AExample A

以通常方式制造具有以下组成的片剂：Tablets with the following composition are manufactured in the usual manner:

表2：可能的片剂组成Table 2: Possible tablet compositions

制造过程：Manufacturing process:

1.将成分1、2、3和4混合，并用纯水粒化。1. Mix ingredients 1, 2, 3 and 4 and granulate with purified water.

2.将颗粒在50℃下干燥。2. Dry the granules at 50°C.

3.使颗粒通过合适的研磨设备。3. Pass the granules through suitable grinding equipment.

4.加入成分5并混合三分钟；在合适的压机上压制。4. Add ingredient 5 and mix for three minutes; compress on a suitable press.

实旆例B-1Example B-1

制备具有以下组成的胶囊：Capsules with the following composition were prepared:

表3：可能的胶囊成分组成Table 3: Possible capsule composition

制造过程：Manufacturing process:

1.将成分1、2和3在合适的混合机中混合30分钟。1. Mix ingredients 1, 2 and 3 in a suitable mixer for 30 minutes.

2.加入成分4和5，并混合3分钟。2. Add ingredients 4 and 5 and mix for 3 minutes.

3.填充至合适的胶囊中。3. Fill into suitable capsules.

将式I化合物、乳糖和玉米淀粉首先在混合机中混合，并且之后在粉碎机中混合。将混合物送回混合机；将滑石加入其中并充分混合。将混合物通过机器填充至合适的胶囊中，例如硬明胶胶囊。The compound of formula I, lactose, and corn starch are first mixed in a mixer and then in a grinder. The mixture is returned to the mixer; talc is added and mixed thoroughly. The mixture is filled into suitable capsules, such as hard gelatin capsules, by machine.

实施例B-2Example B-2

制备具有以下组成的软明胶胶囊：Prepare soft gelatin capsules having the following composition:

成分Element mg/胶囊mg/capsule 式I化合物Compounds of formula I 55 黄蜡Yellow wax 88 氢化大豆油hydrogenated soybean oil 88 部分氢化的植物油Partially hydrogenated vegetable oil 3434 大豆油soybean oil 110110 总计total 165165

表4：可能的软明胶胶囊成分组成Table 4: Possible soft gelatin capsule composition

成分Element mg/胶囊mg/capsule 明胶gelatin 7575 甘油85％Glycerin 85% 3232 Karion 83Karion 83 8(干物质)8 (dry matter) 二氧化钛Titanium dioxide 0.40.4 氧化铁黄Iron oxide yellow 1.11.1 总计total 116.5116.5

表5：可能的软明胶胶囊组成Table 5: Possible soft gelatin capsule compositions

制造过程Manufacturing Process

将式I化合物溶解在其他成分的温暖熔体中，并将混合物填充至适当大小的软明胶胶囊中。根据一般程序处理所填充的软明胶胶囊。The compound of formula I is dissolved in the warm melt of the other ingredients and the mixture is filled into soft gelatin capsules of appropriate size. The filled soft gelatin capsules are processed according to the general procedures.

实施例CExample C

制备以下组成的栓剂：Prepare suppositories of the following composition:

成分Element mg/栓剂mg/suppository 式I化合物Compounds of formula I 1515 栓剂块Suppository blocks 12851285 总计total 13001300

表6：可能的栓剂组成Table 6: Possible suppository compositions

制造过程Manufacturing Process

将栓剂块在玻璃或钢容器中熔化，充分混合并冷却至45℃。之后，将细粉化的式I化合物加入其中并搅拌直至其完全分散。将混合物倒入合适大小的栓剂模具中，放置冷却；之后将栓剂从模具移出并单独地包装在蜡纸或金属箔中。The suppository mass is melted in a glass or steel container, mixed thoroughly and cooled to 45°C. The finely powdered compound of formula I is then added and stirred until completely dispersed. The mixture is poured into a suppository mold of suitable size and allowed to cool; the suppositories are then removed from the mold and individually wrapped in wax paper or metal foil.

实施例DExample D

制备以下组成的针剂：Prepare the following injection:

成分Element mg/针剂mg/injection 式I化合物Compounds of formula I 33 聚乙二醇400polyethylene glycol 400 150150 乙酸Acetic acid 适量至pH 5.0Adjust to pH 5.0 针剂用水Water for injection 至1.0mlto 1.0ml

表7：可能的针剂组成Table 7: Possible injection compositions

制造过程Manufacturing Process

将式I化合物溶解在聚乙二醇400和注射用水(部分)的混合物中。将pH通过乙酸调节至5.0。通过加入余量的水将体积调节至1.0ml。将溶液过滤，使用适当过量装入小瓶中并灭菌。The compound of formula I is dissolved in a mixture of polyethylene glycol 400 and water for injection (part). The pH is adjusted to 5.0 by acetic acid. The volume is adjusted to 1.0 ml by adding a surplus of water. The solution is filtered, filled into a vial with an appropriate excess, and sterilized.

实施例EExample E

制造以下组成的小药囊：Create a sachet of the following composition:

成分Element mg/小药囊mg/sachet 式I化合物Compounds of formula I 5050 乳糖，细粉Lactose, finely powdered 10151015 微晶纤维素(AVICEL PH 102)Microcrystalline cellulose (AVICEL PH 102) 14001400 羧甲基纤维素钠Sodium carboxymethyl cellulose 1414 聚乙烯吡咯烷酮K 30Polyvinylpyrrolidone K 30 1010 硬脂酸镁magnesium stearate 1010 香味添加剂Fragrance additives 11 总计total 25002500

表8：可能的小药囊组成Table 8: Possible sachet compositions

制造过程Manufacturing Process

将式I化合物与乳糖、微晶纤维素和羧甲基纤维素钠混合，并且用聚乙烯吡咯烷酮在水中的混合物造粒。将颗粒与硬脂酸镁和香味添加剂混合，并装入小药囊中。The compound of formula I is mixed with lactose, microcrystalline cellulose and sodium carboxymethylcellulose and granulated with a mixture of polyvinylpyrrolidone in water. The granules are mixed with magnesium stearate and flavoring additives and filled into sachets.

实验部分Experimental part

为说明本发明提供以下实施例。它们不应被认为是作为本发明的范围的限定，而仅作为其代表。The following examples are provided to illustrate the present invention and should not be considered as limiting the scope of the invention, but merely as representative thereof.

通用General

分析方法Analytical methods

使用Agilent 6850Series II单通道GC系统记录气相色谱(GC)。柱：Agilent HP-1，30m x 0.32mm x 0.25μm膜，SN USC174642H，PN 190917-413E；载气：处于恒流模式的氦，压力25psi；标称初始流量7.8mL/min，注射体积1μL；进口：分开(Split)(比例20∶1)；检测器：温度300℃，氢流30mL/min，空气流400mL/min。Gas chromatography (GC) was recorded using an Agilent 6850 Series II single-channel GC system. Column: Agilent HP-1, 30 m x 0.32 mm x 0.25 μm membrane, SN USC174642H, PN 190917-413E; carrier gas: helium in constant flow mode, pressure 25 psi; nominal initial flow 7.8 mL/min, injection volume 1 μL; inlet: split (20:1 ratio); detector: temperature 300° C., hydrogen flow 30 mL/min, air flow 400 mL/min.

温箱温度程序：Incubator temperature program:

时间[min]Time [min] 起始温度[℃]Starting temperature [℃] 速率[℃/min]Rate [℃/min] 终末温度[℃]Final temperature [℃] 0.00.0 4040 5.05.0 8080 8.08.0 8080 20.020.0 250250 16.516.5 -方法的最后--The end of the method-

HPLC(方法LCMS_fglm)HPLC (method LCMS_fglm)

柱：Agilent Zorbax Eclipse Plus C18，快速解析HT，2.1x30mm，1.8μm，部件号(Part.no.)959731-902Column: Agilent Zorbax Eclipse Plus C18, Rapid Resolution HT, 2.1x30 mm, 1.8 μm, Part No. 959731-902

溶剂A：水0.01％甲酸；溶剂B：乙腈(MeCN)Solvent A: water 0.01% formic acid; Solvent B: acetonitrile (MeCN)

梯度：gradient:

时间[min]Time [min] 流速[ml/min]Flow rate [ml/min] ％A%A ％B%B 起始Start 0.80.8 9797 33 0.20.2 1.01.0 9797 33 1.71.7 1.01.0 33 9797 2.02.0 1.01.0 33 9797 2.12.1 1.01.0 9797 33

HPLC(方法LCMS_梯度)HPLC (method LCMS_gradient)

柱：Agilent Zorbax Eclipse Plus C18，快速解析HT，2.1x30mm，1.8μm，部件号959731-902Column: Agilent Zorbax Eclipse Plus C18, Rapid Resolution HT, 2.1 x 30 mm, 1.8 μm, part number 959731-902

溶剂A：水0.01％甲酸；溶剂B：MeCNSolvent A: water 0.01% formic acid; Solvent B: MeCN

梯度：gradient:

时间[min]Time [min] 流速[ml/min]Flow rate [ml/min] ％A%A ％B%B 起始Start 1.01.0 9797 33 0.20.2 1.01.0 9797 33 5.25.2 1.01.0 33 9797 6.06.0 1.01.0 33 9797 6.26.2 1.01.0 9797 33

HPLC(方法7626L05)HPLC (Method 7626L05)

柱：Agilent Poroshell 120EC-C18，4.6x50mm，2.7μm，部件号699975-902Column: Agilent Poroshell 120EC-C18, 4.6 x 50 mm, 2.7 μm, p/n 699975-902

溶剂A：MeCN；溶剂B：水/MeCN 95∶5v/v；溶剂C：1g四正丁基硫酸氢铵在1L水中/MeCN 1∶4v/v中的溶液。Solvent A: MeCN; Solvent B: water/MeCN 95:5 v/v; Solvent C: a solution of 1 g of tetra-n-butylammonium hydrogen sulfate in 1 L of water/MeCN 1:4 v/v.

梯度：gradient:

时间[min]Time [min] 流速[ml/min]Flow rate [ml/min] ％A%A ％B%B ％C%C 起始Start 1.01.0 1010 8585 55 1.01.0 1.01.0 1010 8585 55 7.07.0 1.01.0 8585 1010 55 12.012.0 1.01.0 8585 1010 55 13.013.0 1.01.0 1010 8585 55

缩写abbreviation

以下缩写用于实验部分：THF，四氢呋喃；MTBE，甲基-叔丁醚；DMF，二甲基甲酰胺；TLC，薄层色谱。The following abbreviations are used in the experimental part: THF, tetrahydrofuran; MTBE, methyl-tert-butyl ether; DMF, dimethylformamide; TLC, thin layer chromatography.

中间体intermediates

Int-7：2-甲基-2-[S-甲基-N-(3-四氢吡喃-2-基氧基丙基)磺酰亚胺基]丙腈的合成Int-7: Synthesis of 2-methyl-2-[S-methyl-N-(3-tetrahydropyran-2-yloxypropyl)sulfonylimino]propionitrile

步骤1：2-甲基-2-甲基硫基-丙腈(Int-2)Step 1: 2-Methyl-2-methylsulfanyl-propionitrile (Int-2)

将氢化钠(24.0g，60％悬浮液于矿物油中，600mmol)用正庚烷(3x 100mL)洗涤并且在0-5℃悬浮在THF(300mL)中。加入2-(甲硫基)乙腈(Int-1，20g，230mmol)在THF(100mL)中的溶液并且将得到的悬浮液在0-5℃(冰浴)搅拌15min。然后，经15min加入甲基碘(90.8g，40.0mL，640mmol)在THF中的溶液。让混合物加温并在室温搅拌3h。之后，将反应混合物小心倒到水(200mL)上并且用MTBE(1x 500mL，3x 150mL)萃取。将合并的萃取物用饱和碳酸氢钠水溶液(100mL)和盐水(100mL)洗涤，干燥(硫酸钠)并且在真空中浓缩，在真空中干燥(10毫巴，40℃，45min)后，得到标题化合物，为黄色油状物(23.4g，89％)，其在不进一步纯化的情况下用于下一步骤。GC(方法7626G01)t_R＝2.5min。¹H NMR(CDCl₃，400MHz)：δ1.65(s，6H)，2.32(s，3H)。Sodium hydride (24.0g, 60% suspension in mineral oil, 600mmol) is washed with n-heptane (3x 100mL) and suspended in THF (300mL) at 0-5°C. A solution of 2-(methylthio)acetonitrile (Int-1, 20g, 230mmol) in THF (100mL) is added and the resulting suspension is stirred at 0-5°C (ice bath) for 15min. Then, a solution of methyl iodide (90.8g, 40.0mL, 640mmol) in THF is added over 15min. The mixture is heated and stirred at room temperature for 3h. Afterwards, the reaction mixture is carefully poured onto water (200mL) and extracted with MTBE (1x 500mL, 3x 150mL). The combined extracts were washed with saturated aqueous sodium bicarbonate solution (100 mL) and brine (100 mL), dried (sodium sulfate) and concentrated in vacuo to afford, after drying in vacuo (10 mbar, 40° C., 45 min), the title compound as a yellow oil (23.4 g, 89%), which was used in the next step without further purification. GC (Method 7626G01) t _R = 2.5 min. ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.65 (s, 6H), 2.32 (s, 3H).

步骤2：2-甲基-2-甲基亚硫酰基-丙腈(Int-3)Step 2: 2-Methyl-2-methylsulfinyl-propionitrile (Int-3)

将2-甲基-2-(甲硫基)丙腈(Int-2，23g，200mmol)溶解在1，4-二烷(115mL)中并加入水(345mL)。将乳状液冷却至0-5℃(冰浴)并将过碘酸钠(44.8g，210mmol)与水(115mL)一起加入。将得到的白色悬浮液加温至室温并剧烈搅拌16h。然后，过滤混合物，将残留物用乙酸乙酯(400mL)洗涤。滤液相分离后，将水相用氯化钠饱和，用乙酸乙酯(5x 200mL)萃取。将合并的萃取物用盐水(100mL)洗涤，干燥(硫酸钠)并且在真空中浓缩，干燥(50℃，5毫巴)后得到标题化合物，为黄色油状物(25g)。将粗产物通过柱色谱(硅胶，100g，用乙酸乙酯/正庚烷，梯度50∶50至0∶100洗脱)纯化，得到标题化合物，为黄色油状物(23.3g，89％)。¹H NMR(CDCl₃，400MHz)：δ1.59(s，3H)，1.68(s，3H)，2.74(s，3H)。2-methyl-2-(methylthio)propionitrile (Int-2,23g, 200mmol) is dissolved in 1,4-dioxane (115mL) and water (345mL) is added. The emulsion is cooled to 0-5 ℃ (ice bath) and sodium periodate (44.8g, 210mmol) is added together with water (115mL). The white suspension obtained is heated to room temperature and stirred vigorously for 16h. Then, the mixture is filtered and the residue is washed with ethyl acetate (400mL). After the filtrate is separated, the aqueous phase is saturated with sodium chloride and extracted with ethyl acetate (5x 200mL). The combined extracts are washed with salt water (100mL), dried (sodium sulfate) and concentrated in vacuo, and the title compound is obtained after drying (50 ℃, 5 mbar) as a yellow oil (25g). The crude product was purified by column chromatography (silica gel, 100 g, eluted with ethyl acetate/n-heptane, gradient 50:50 to 0:100) to give the title compound as a yellow oil (23.3 g, 89%). ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.59 (s, 3H), 1.68 (s, 3H), 2.74 (s, 3H).

步骤3：2-甲基-2-(S-甲基磺酰(三氟乙酰基亚胺基))丙腈(Int-4)Step 3: 2-Methyl-2-(S-methylsulfonyl(trifluoroacetylimino))propionitrile (Int-4)

在0-5℃(冰浴)将2-甲基-2-(甲基亚硫酰基)丙腈(Int-3，9.8g，74.7mmol)溶解在二氯甲烷(390mL)中，并且接着加入2，2，2-三氟乙酰胺(17.0g，151mmol)，氧化镁(12.7g，307mmol)和乙酸铑(II)二聚物(850mg，1.92mmol)。最后，加入碘苯二乙酸酯(36.3g，113mmol)在二氯甲烷(98.0mL)中的溶液并且将混合物在0-5℃搅拌1h，接着在室温搅拌6h。然后，加入第二部分乙酸铑(II)二聚物(850mg，1.92mmol)并且将悬浮液在室温搅拌另外95h。过滤反应混合物，将残留物用二氯甲烷(100mL)洗涤并且将合并的滤液在真空中浓缩，得到深色油状物，为粗产物。柱色谱(硅胶，100g，用乙酸乙酯/正庚烷，梯度10∶90至50∶50洗脱)和在真空中干燥(50℃，5毫巴)后，分离标题化合物，为浅黄色油状物，其在静置后固化(13.74g，76％)。HPLC(方法LCMS_fglm)t_R＝0.97min。¹H NMR(CDCl₃，400MHz)：δ1.91(s，3H)，1.91(s，3H)，3.65(s，3H)。MS(ES-)m/z 241.1[M-H]。2-Methyl-2-(methylsulfinyl)propionitrile (Int-3, 9.8 g, 74.7 mmol) was dissolved in dichloromethane (390 mL) at 0-5° C. (ice bath), and then 2,2,2-trifluoroacetamide (17.0 g, 151 mmol), magnesium oxide (12.7 g, 307 mmol) and rhodium(II) acetate dimer (850 mg, 1.92 mmol) were added. Finally, a solution of iodobenzene diacetate (36.3 g, 113 mmol) in dichloromethane (98.0 mL) was added and the mixture was stirred at 0-5° C. for 1 h, then at room temperature for 6 h. Then, a second portion of rhodium(II) acetate dimer (850 mg, 1.92 mmol) was added and the suspension was stirred at room temperature for another 95 h. The reaction mixture was filtered, the residue washed with dichloromethane (100 mL), and the combined filtrates were concentrated in vacuo to give a dark oil as the crude product. After column chromatography (silica gel, 100 g, eluting with ethyl acetate/n-heptane, gradient 10:90 to 50:50) and drying in vacuo (50° C., 5 mbar), the title compound was isolated as a light yellow oil that solidified upon standing (13.74 g, 76%). HPLC (Method LCMS_fglm) _tR = 0.97 min. ^1H NMR (CDCl ₃ , 400 MHz): δ 1.91 (s, 3H), 1.91 (s, 3H), 3.65 (s, 3H). MS (ES-) m/z 241.1 [MH].

步骤4：2-甲基-2-(S-甲磺酰亚胺基)丙腈(Int-5)Step 4: 2-Methyl-2-(S-methylsulfonylimino)propionitrile (Int-5)

在0-5℃(冰浴)将2-甲基-2-(S-甲磺酰(三氟乙酰基亚胺基))丙腈(Int-4，9.90g，40.9mmol)溶解在甲醇(100mL)中。将碳酸钾(28.2g，204mmol)与甲醇(20mL)一起加入并且将得到的悬浮液在室温搅拌0.5h。将反应混合物用MTBE(250mL)稀释并且加入硅胶(25g)，将混合物搅拌15min。之后，将其经硅胶塞(35g)过滤，将残留物用MTBE/甲醇2∶1(v/v，250mL)洗涤。将合并的滤液在真空中浓缩，得到黄色油状物。再次将该材料溶解在乙酸乙酯(200mL)中并且经硅胶塞(40g)过滤，将残留物用乙酸乙酯(200mL)洗涤。将合并的滤液再次在真空中浓缩，得到粗产物，为黄色油状物(5.98g)。将粗制物通过柱色谱(硅胶，100g，用乙酸乙酯/正庚烷，梯度50∶50至100∶0洗脱)纯化，在真空中干燥(50℃，5毫巴)后得到标题化合物，为浅黄色固体(4.54g，76％)。GC(方法7626G01)t_R＝9.7min。¹H NMR(CDCl₃，400MHz)：δ1.75(s，3H)，1.76(s，3H)，2.79(br s，1H)，3.15(s，3H)。2-Methyl-2-(S-methylsulfonyl(trifluoroacetylimino))propionitrile (Int-4, 9.90 g, 40.9 mmol) was dissolved in methanol (100 mL) at 0-5°C (ice bath). Potassium carbonate (28.2 g, 204 mmol) was added together with methanol (20 mL) and the resulting suspension was stirred at room temperature for 0.5 h. The reaction mixture was diluted with MTBE (250 mL) and silica gel (25 g) was added, and the mixture was stirred for 15 min. Afterwards, it was filtered through a silica gel plug (35 g) and the residue was washed with MTBE/methanol 2: 1 (v/v, 250 mL). The combined filtrate was concentrated in vacuo to give a yellow oil. The material was again dissolved in ethyl acetate (200 mL) and filtered through a silica gel plug (40 g), and the residue was washed with ethyl acetate (200 mL). The combined filtrate was concentrated in vacuo again to give the crude product as a yellow oil (5.98 g). The crude product was purified by column chromatography (silica gel, 100 g, elution with ethyl acetate/n-heptane, gradient 50:50 to 100:0) to afford, after drying in vacuo (50° C., 5 mbar), the title compound as a light yellow solid (4.54 g, 76%). GC (Method 7626G01) t _R = 9.7 min. ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.75 (s, 3H), 1.76 (s, 3H), 2.79 (br s, 1H), 3.15 (s, 3H).

步骤5：2-甲基-2-[S-甲基-N-(3-四氢吡喃-2-基氧基丙基)磺酰亚胺基]丙腈(Int-7)Step 5: 2-Methyl-2-[S-methyl-N-(3-tetrahydropyran-2-yloxypropyl)sulfonylimino]propionitrile (Int-7)

将氢化钾(30％w/w于矿物油中，2.2g，16.5mmol)悬浮在1，2-二甲氧基乙烷(20.0mL)中并且将悬浮液冷却至0-5℃(冰浴)。经10min逐滴加入2-甲基-2-(甲基磺酰亚胺基)丙腈(Int-5，2.0g，13.7mmol)在1，2-二甲氧基乙烷(15mL)中的溶液。去除冰浴并且将混合物在室温搅拌3h。之后，将其再次冷却至0-5℃并且加入丁基溴化铵(235mg，730μmol)，接着加入2-(3-溴丙氧基)四氢-2H-吡喃(Int-6，3.95g，3.00mL，17.7mmol)。将反应混合物在室温搅拌16h。然后，将混合物倒到饱和碳酸氢钠水溶液(60mL)上并且用乙酸乙酯(180mL)稀释。相分离后，将水相用乙酸乙酯(2x 50mL)萃取，将合并的有机萃取物干燥(硫酸钠)并在真空中浓缩，得到黄色双相油状物，为粗产物。将其通过柱色谱(硅胶，100g，用乙酸乙酯/正庚烷，梯度40∶60至100∶0洗脱)纯化，在在真空中干燥(50℃，5毫巴)后，得到标题化合物，为非对映异构体的混合物，为无色粘稠油状物(3.28g，83％)。HPLC(方法LCMS_梯度)t_R＝1.6min。¹H NMR(CDCl₃，400MHz)：δ1.47-1.60(m，4H)，1.68-1.77(m，1H)，1.76(br s，6H)，1.78-1.88(m，3H)，3.06&3.07(2s，3H，非对映异构(diast.))，3.20-3.41(m，2H)，3.45-3.54(m，2H)，3.79-3.91(m，2H)，4.56-4.60(m，1H)。MS(ES+)m/z 205.1[M+H-(C₅H₈O)]。Potassium hydride (30% w / w in mineral oil, 2.2g, 16.5mmol) is suspended in 1,2-dimethoxyethane (20.0mL) and the suspension is cooled to 0-5 ° C (ice bath). A solution of 2-methyl-2-(methylsulfonylimino)propionitrile (Int-5, 2.0g, 13.7mmol) in 1,2-dimethoxyethane (15mL) is added dropwise over 10min. The ice bath is removed and the mixture is stirred at room temperature for 3h. Afterwards, it is cooled to 0-5 ° C again and butylammonium bromide (235mg, 730μmol) is added, followed by 2-(3-bromopropoxy)tetrahydro-2H-pyran (Int-6, 3.95g, 3.00mL, 17.7mmol). The reaction mixture is stirred at room temperature for 16h. Then, the mixture is poured onto a saturated aqueous sodium bicarbonate solution (60mL) and diluted with ethyl acetate (180mL). After phase separation, the aqueous phase was extracted with ethyl acetate (2 x 50 mL), and the combined organic extracts were dried (sodium sulfate) and concentrated in vacuo to give a yellow biphasic oil as the crude product. This was purified by column chromatography (silica gel, 100 g, eluted with ethyl acetate/n-heptane, gradient 40: 60 to 100: 0) to give the title compound as a mixture of diastereomers as a colorless viscous oil (3.28 g, 83%) after drying in vacuo (50° C., 5 mbar). HPLC (method LCMS_gradient) t _R = 1.6 min. ^1H NMR (CDCl ₃ , 400 MHz): δ 1.47-1.60 (m, 4H), 1.68-1.77 (m, 1H), 1.76 (br s, 6H), 1.78-1.88 (m, 3H), 3.06 & 3.07 (2s, 3H, diastereomers), 3.20-3.41 (m, 2H), 3.45-3.54 (m, 2H), 3.79-3.91 (m, 2H), 4.56-4.60 (m, 1H). MS (ES+) m/z 205.1 [M+H-(C ₅ H ₈ O)].

Int-11A和Int-11B：2-((R，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-(3-羟基丙基)丙基磺酰亚胺基)-2-甲基丙腈(Int-11A)和2-((S，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-(3-羟基丙基)丙基磺酰亚胺基)-2-甲基丙腈(Int-11B)的合成Int-11A and Int-11B: Synthesis of 2-((R,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-(3-hydroxypropyl)propylsulfonylimino)-2-methylpropionitrile (Int-11A) and 2-((S,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-(3-hydroxypropyl)propylsulfonylimino)-2-methylpropionitrile (Int-11B)

步骤1：N-[1-(6-溴-3-氟-4-三乙基甲硅烷基-2-吡啶基)-2-[S-(1-氰基-1-甲基-乙基)-N-(3-四氢吡喃-2-基氧基丙基)磺酰亚胺基]-1-甲基-乙基]-2-甲基-丙-2-亚磺酰胺(Int-10(混合物))Step 1: N-[1-(6-Bromo-3-fluoro-4-triethylsilyl-2-pyridyl)-2-[S-(1-cyano-1-methyl-ethyl)-N-(3-tetrahydropyran-2-yloxypropyl)sulfonylimino]-1-methyl-ethyl]-2-methyl-propane-2-sulfenamide (Int-10 (mixture))

将2-甲基-2-(S-甲基-N-(3-(四氢-2H-吡喃-2-基氧基)丙基)磺酰亚胺基)丙腈(Int-8，3.31g，11.5mmol)溶解在THF(45mL)中并且将溶液冷却至＜-70℃(丙酮/干冰浴)。经10min逐滴加入正丁基锂(1.6M于己烷中，7.0mL，11.2mmol)，并且将得到的溶液在＜-70℃搅拌50min。然后，在该温度经20min加入(R，E)-N-(1-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)亚乙基)-2-甲基丙-2-亚磺酰胺(Int-9，3.90g，8.96mmol)在THF(45.0mL)中的溶液。在＜70℃搅拌30min后，将反应混合物倒到2M氯化铵水溶液(75mL)上并用MTBE(1x300mL，2x 100mL)萃取。将合并的有机萃取物在真空中浓缩，得到黄色粘性油状物(2.46g)，其在不进一步纯化的情况下用于下一步骤。2-Methyl-2-(S-methyl-N-(3-(tetrahydro-2H-pyran-2-yloxy)propyl)sulfonylimino)propionitrile (Int-8, 3.31 g, 11.5 mmol) was dissolved in THF (45 mL) and the solution was cooled to <-70°C (acetone/dry ice bath). n-Butyllithium (1.6 M in hexanes, 7.0 mL, 11.2 mmol) was added dropwise over 10 min, and the resulting solution was stirred at <-70°C for 50 min. Then, a solution of (R,E)-N-(1-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)ethylidene)-2-methylpropane-2-sulfenamide (Int-9, 3.90 g, 8.96 mmol) in THF (45.0 mL) was added at this temperature over 20 min. After stirring at <70°C for 30 min, the reaction mixture was poured onto 2M aqueous ammonium chloride solution (75 mL) and extracted with MTBE (1 x 300 mL, 2 x 100 mL). The combined organic extracts were concentrated in vacuo to give a yellow viscous oil (2.46 g) which was used in the next step without further purification.

步骤2：2-((R，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-(3-羟基丙基)丙基磺酰亚胺基)-2-甲基丙腈(Int-11A)和2-((S，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-(3-羟基丙基)丙基磺酰亚胺基)-2-甲基丙腈(Int-11B)Step 2: 2-((R,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-(3-hydroxypropyl)propylsulfonylimino)-2-methylpropionitrile (Int-11A) and 2-((S,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-(3-hydroxypropyl)propylsulfonylimino)-2-methylpropionitrile (Int-11B)

将N-[1-(6-溴-3-氟-4-三乙基甲硅烷基-2-吡啶基)-2-[S-(1-氰基-1-甲基-乙基)-N-(3-四氢吡喃-2-基氧基丙基)磺酰亚胺基]-1-甲基-乙基]-2-甲基-丙-2-亚磺酰胺(Int-10(混合物)，7.40g，10.2mmol)溶解在乙醇(74mL)中并且在0-5℃(冰浴)加入氯化氢的甲醇溶液(约20％w/w，30g，30mL，165mmol)。将混合物在室温搅拌30min。之后，将其倒到2M碳酸钠水溶液(200mL)上并用MTBE(300mL)稀释。将得到的悬浮液过滤，将残留物用MTBE(100mL)洗涤。分离合并的滤液的相，将水相用乙酸乙酯(2x 100mL)萃取，将合并的有机萃取物干燥(硫酸钠)并在真空中浓缩，得到褐色油状物，为粗产物。将粗产物通过柱色谱(硅胶，100g，用乙酸乙酯/甲醇，梯度100∶0至90∶10洗脱)纯化，在真空中干燥(55℃，5毫巴)后得到标题化合物，为分离的非对映异构体。将首先洗脱的少量的非对映异构体(Int-11B)分离，为无色粘性油状物(920mg，17％)，获得其次洗脱的主要的非对映异构体(Int-11A)，为无色油状物(3.42g，62％)。经两步的总体产率是79％。N-[1-(6-bromo-3-fluoro-4-triethylsilyl-2-pyridyl)-2-[S-(1-cyano-1-methyl-ethyl)-N-(3-tetrahydropyran-2-yloxypropyl)sulfonylimino]-1-methyl-ethyl]-2-methyl-propane-2-sulfinamide (Int-10 (mixture), 7.40 g, 10.2 mmol) was dissolved in ethanol (74 mL) and a methanolic solution of hydrogen chloride (about 20% w/w, 30 g, 30 mL, 165 mmol) was added at 0-5° C. (ice bath). The mixture was stirred at room temperature for 30 min. Afterwards, it was poured onto a 2M aqueous sodium carbonate solution (200 mL) and diluted with MTBE (300 mL). The resulting suspension was filtered and the residue was washed with MTBE (100 mL). The phases of the combined filtrates were separated, and the aqueous phase was extracted with ethyl acetate (2 x 100 mL). The combined organic extracts were dried (sodium sulfate) and concentrated in vacuo to give a brown oil as the crude product. The crude product was purified by column chromatography (silica gel, 100 g, eluted with ethyl acetate/methanol, gradient 100:0 to 90:10) and dried in vacuo (55° C., 5 mbar) to give the title compound as the separated diastereomers. The first eluting minor diastereomer (Int-11B) was separated as a colorless viscous oil (920 mg, 17%), and the second eluting major diastereomer (Int-11A) was obtained as a colorless oil (3.42 g, 62%). The overall yield over two steps was 79%.

Int-11A：HPLC(方法LCMS_梯度)t_R＝2.1min。¹H NMR(CDCl₃，400MHz)：δ0.82-0.91(m，6H)，0.94-1.01(m，9H)，1.57(d，J＝0.8Hz，3H)，1.59-1.66(m，2H)，1.68(s，3H)，1.72(s，3H)，2.77(br s，3H)，3.10-3.18(m，1H)，3.24-3.31(m，1H)，3.57-3.71(m，2H)，3.80(dd，J＝1.1，13.7Hz，1H)，4.08(d，J＝13.7Hz，1H)，7.35(d，J＝2.7Hz，1H)。MS(ES+)m/z 537.3&535.3[M+H]。Int-11A: HPLC (method LCMS_gradient) _tR = 2.1 min. ¹ H NMR (CDCl ₃ , 400MHz): δ0.82-0.91 (m, 6H), 0.94-1.01 (m, 9H), 1.57 (d, J=0.8Hz, 3H), 1.59-1.66 (m, 2H), 1.68 (s, 3H), 1.72 (s, 3H), 2.77 (br s, 3H), 3.10-3.18 (m, 1H), 3.24-3.31 (m, 1H), 3.57-3.71 (m, 2H), 3.80 (dd, J=1.1, 13.7Hz, 1H), 4.08 (d, J=13.7Hz, 1H), 7.35 (d, J=2.7Hz, 1H). MS(ES+)m/z 537.3&535.3[M+H].

Int-11B：HPLC(方法LCMS_梯度)t_R＝2.1min。¹H NMR(CDCl₃，400MHz)：δ0.82-0.90(m，6H)，0.93-1.00(m，9H)，1.31-1.40(m，1H)，1.42-1.51(m，1H)，1.62(d，J＝1.1Hz，3H)，1.71(s，3H)，1.75(s，3H)，2.31(brs，3H)，3.05(ddd，J＝5.5，6.3，11.9Hz，1H)，3.26(ddd，J＝5.1，7.5，12.4Hz，1H)，3.54(t，J＝5.9Hz，2H)，3.63(d，J＝13.7Hz，1H)，4.22(d，J＝13.4Hz，1H)，7.33(d，J＝2.7Hz，1H)。MS(ES+)m/z 537.3&535.3[M+H]。Int-11B: HPLC (method LCMS_gradient) t _R = 2.1 min. ¹ H NMR (CDCl ₃ , 400 MHz): δ 0.82-0.90 (m, 6H), 0.93-1.00 (m, 9H), 1.31-1.40 (m, 1H), 1.42-1.51 (m, 1H), 1.62 (d, J=1.1 Hz, 3H), 1.71 (s, 3H), 1.75 (s, 3H), 2.31 (brs, 3H), 3.05 ( ddd, J=5.5, 6.3, 11.9Hz, 1H), 3.26 (ddd, J=5.1, 7.5, 12.4Hz, 1H), 3.54 (t, J=5. 9Hz, 2H), 3.63 (d, J=13.7Hz, 1H), 4.22 (d, J=13.4Hz, 1H), 7.33 (d, J=2.7Hz, 1H). MS(ES+)m/z 537.3&535.3[M+H].

Int-14A：4-甲基苯磺酸3-(((1R，3R)-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5-((叔丁氧基羰基)氨基)-3，6，6-三甲基-1-氧代-3，6-二氢-2H-1，4-噻嗪-1-亚基)氨基)丙酯的合成Int-14A: Synthesis of 3-(((1R,3R)-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5-((tert-butoxycarbonyl)amino)-3,6,6-trimethyl-1-oxo-3,6-dihydro-2H-1,4-thiazin-1-ylidene)amino)propyl 4-methylbenzenesulfonate

步骤1：(1R，3R)-5-氨基-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((3-羟基丙基)亚氨基)-3，6，6-三甲基-3，6-二氢-2H-1，4-噻嗪1-氧化物(Int-12A)Step 1: (1R,3R)-5-amino-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((3-hydroxypropyl)imino)-3,6,6-trimethyl-3,6-dihydro-2H-1,4-thiazine 1-oxide (Int-12A)

将2-((R，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-(3-羟基丙基)丙基磺酰亚胺基)-2-甲基丙腈(Int-11A，3.40g，6.35mmol)溶解在乙醇(59mL)中并加入氯化铜(I)(660mg，6.67mmol)。将混合物加热至75-80℃并且在该温度搅拌45min。之后，将其冷却至5℃(冰浴)并且倒到盐水(40mL)和氨水(25％w/w，20mL)的混合物上。将得到的混合物用MTBE(200mL)和乙酸乙酯(2x 50mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩，得到黄色油状物，为粗产物。将粗产物通过柱色谱(硅胶，100g，用乙酸乙酯/甲醇，梯度95∶5至85∶15洗脱)纯化，在在真空中干燥(55℃，5毫巴)后得到标题化合物，为白色固体(2.79g，82％)。HPLC(方法LCMS_梯度)t_R＝1.8min。¹H NMR(CDCl₃，400MHz)：δ0.81-0.89(m，6H)，0.92-0.99(m，9H)，1.40-1.65(m，2H)，1.63(s，3H)，1.73(s，3H)，1.78(s，3H)，2.78-2.88(m，1H)，3.16-3.24(m，1H)，3.28-3.36(m，1H)，3.39(d，J＝14.8Hz，1H)，3.41-3.49(m，1H)，3.56-3.70(m，2H)，3.78-3.82(m，1H)，4.24(d，J＝14.8Hz，1H)，7.32(d，J＝2.4Hz，1H)。MS(ES+)m/z 537.3&535.3[M+H，Br]。2-((R,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-(3-hydroxypropyl)propylsulfonylimino)-2-methylpropionitrile (Int-11A, 3.40 g, 6.35 mmol) was dissolved in ethanol (59 mL) and copper(I) chloride (660 mg, 6.67 mmol) was added. The mixture was heated to 75-80° C. and stirred at this temperature for 45 min. Afterwards, it was cooled to 5° C. (ice bath) and poured onto a mixture of brine (40 mL) and aqueous ammonia (25% w/w, 20 mL). The resulting mixture was extracted with MTBE (200 mL) and ethyl acetate (2 x 50 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo to give a yellow oil as the crude product. The crude product was purified by column chromatography (silica gel, 100 g, eluted with ethyl acetate/methanol, gradient 95:5 to 85:15) to give the title compound after drying in vacuo (55° C., 5 mbar) as a white solid (2.79 g, 82%). HPLC (method LCMS_gradient) t _R =1.8 min. ¹ H NMR (CDCl ₃ , 400MHz): δ0.81-0.89 (m, 6H), 0.92-0.99 (m, 9H), 1.40-1.65 (m, 2H), 1.6 3(s, 3H), 1.73(s, 3H), 1.78(s, 3H), 2.78-2.88(m, 1H), 3.16-3.24(m, 1H), 3.28-3.36 (m, 1H), 3.39 (d, J=14.8Hz, 1H), 3.41-3.49 (m, 1H), 3.56-3.70 (m, 2H), 3.78-3.82 (m, 1H), 4.24 (d, J=14.8Hz, 1H), 7.32 (d, J=2.4Hz, 1H). MS(ES+)m/z 537.3&535.3[M+H, Br].

步骤2：((1R，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((3-羟基丙基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-13A)Step 2: tert-Butyl ((1R,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((3-hydroxypropyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-13A)

将(1R，3R)-5-氨基-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((3-羟基丙基)亚氨基)-3，6，6-三甲基-3，6-二氢-2H-1，4-噻嗪1-氧化物(Int-12A，2.20g，4.11mmol)溶解在THF(22mL)和水(4.4mL)中并且加入Boc-酐(1g，1.06ml，4.58mmol)，碳酸氢钠(440mg，5.24mmol)和4-二甲基氨基吡啶(26mg，213μmol)。将混合物在室温搅拌1.5h。之后，加入氨水(25％w/w，198mg，220μl，2.91mmol)并将混合物搅拌另外30min。然后将反应混合物倒到水(50mL)上并用MTBE(1x 150mL，1x 50mL)萃取。将合并的萃取物干燥并在真空中浓缩。将粗产物通过柱色谱(硅胶，100g，用乙酸乙酯/正庚烷，梯度40∶60至60∶40洗脱)纯化，在真空中干燥(50℃，5毫巴)后，得到标题化合物，为无色胶质(2.06g，79％)。HPLC(方法LCMS_梯度)t_R＝3.9min。¹H NMR(CDCl₃，400MHz)：δ0.82-0.90(m，6H)，0.93-0.99(m，9H)，1.37-1.61(m，2H)，1.54(s，9H)，1.60(s，3H)，1.63(s，3H)，1.77(s，3H)，2.65-2.74(m，2H)，3.13-3.20(m，1H)，3.54(d，J＝15.0Hz，1H)，3.57-3.69(m，2H)，4.48(d，J＝15.3Hz，1H)，7.37(d，J＝2.7Hz，1H)，11.14(br s，1H，exch)。MS(ES+)m/z 637.4&635.6[M+H，Br]。(1R,3R)-5-amino-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((3-hydroxypropyl)imino)-3,6,6-trimethyl-3,6-dihydro-2H-1,4-thiazine 1-oxide (Int-12A, 2.20 g, 4.11 mmol) was dissolved in THF (22 mL) and water (4.4 mL) and Boc-anhydride (1 g, 1.06 ml, 4.58 mmol), sodium bicarbonate (440 mg, 5.24 mmol) and 4-dimethylaminopyridine (26 mg, 213 μmol) were added. The mixture was stirred at room temperature for 1.5 h. Afterwards, aqueous ammonia (25% w/w, 198 mg, 220 μl, 2.91 mmol) was added and the mixture was stirred for another 30 min. The reaction mixture was then poured onto water (50 mL) and extracted with MTBE (1 x 150 mL, 1 x 50 mL). The combined extracts were dried and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 100 g, eluted with ethyl acetate/n-heptane, gradient 40: 60 to 60: 40) and dried in vacuo (50° C., 5 mbar) to give the title compound as a colorless gum (2.06 g, 79%). HPLC (method LCMS-gradient) t _R = 3.9 min. ¹ H NMR (CDCl ₃ , 400MHz): δ0.82-0.90 (m, 6H), 0.93-0.99 (m, 9H), 1.37-1.61 (m, 2H), 1.54 (s, 9H), 1.60 (s, 3H), 1.63 (s, 3H), 1.77 (s, 3H), 2.65-2 .74 (m, 2H), 3.13-3.20 (m, 1H), 3.54 (d, J = 15.0Hz, 1H), 3.57-3.69 (m, 2H), 4.48 (d, J = 15.3Hz, 1H), 7.37 (d, J = 2.7Hz, 1H), 11.14 (br s, 1H, exch). MS(ES+)m/z 637.4&635.6[M+H, Br].

步骤3：4-甲基苯磺酸3-(((1R，3R)-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5-((叔丁氧基羰基)氨基)-3，6，6-三甲基-1-氧代-3，6-二氢-2H-1，4-噻嗪-1-亚基)氨基)丙酯(Int-14A)Step 3: 3-(((1R,3R)-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5-((tert-butoxycarbonyl)amino)-3,6,6-trimethyl-1-oxo-3,6-dihydro-2H-1,4-thiazin-1-ylidene)amino)propyl 4-methylbenzenesulfonate (Int-14A)

将((1R，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((3-羟基丙基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-13A，2.0g，3.15mmol)溶解在二氯甲烷(20mL)中，将溶液冷却至0-5℃(冰浴)并且接着在该温度加入三乙胺(799mg，1.1mL，7.89mmol)，甲苯磺酰氯(925mg，4.85mmol)在二氯甲烷(10ml)中的溶液和4-二甲基氨基吡啶(30mg，246μmol)。将得到的澄清溶液在室温搅拌16h。然后将反应混合物倒到饱和氯化铵水溶液(50mL)上，并且在相分离后，将水相用MTBE(1x 200mL，1x50mL)萃取。将合并的萃取物用饱和碳酸氢钠水溶液(50mL)洗涤，干燥(硫酸钠)并在真空中浓缩，得到混浊褐色油状物，为粗产物。将粗产物通过柱色谱(硅胶，100g，用乙酸乙酯/正庚烷，梯度25∶75至40∶60洗脱)纯化，在在真空中干燥(50℃，5毫巴)后，得到标题化合物，为白色固体(2.29g，92％)。HPLC(方法LCMS_梯度)t_R＝4.6min。¹H NMR(CDCl₃，400MHz)：δ0.81-0.89(m，6H)，0.92-0.98(m，9H)，1.46(s，3H)，1.48-1.69(m，2H)，1.54(s，9H)，1.59(s，3H)，1.70(s，3H)，2.44(s，3H)，2.50-2.60(m，1H)，2.93-3.02(m，1H)，3.49(d，J＝15.0Hz，1H)，3.95-4.02(m，2H)，4.39(d，J＝15.3Hz，1H)，7.33(d，J＝8.1Hz，2H)，7.36(d，J＝2.4Hz，1H)，7.76(d，J＝8.3Hz，2H)，11.10(br s，1H，exch)。MS(ES+)m/z 791.4&789.6[M+H，Br]。Tert-butyl ((1R,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((3-hydroxypropyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-13A, 2.0 g, 3.15 mmol) was dissolved in dichloromethane (20 mL), the solution was cooled to 0-5° C. (ice bath) and then triethylamine (799 mg, 1.1 mL, 7.89 mmol), a solution of tosyl chloride (925 mg, 4.85 mmol) in dichloromethane (10 ml) and 4-dimethylaminopyridine (30 mg, 246 μmol) were added at this temperature. The resulting clear solution was stirred at room temperature for 16 h. The reaction mixture was then poured onto a saturated aqueous ammonium chloride solution (50 mL) and, after phase separation, the aqueous phase was extracted with MTBE (1 x 200 mL, 1 x 50 mL). The combined extracts were washed with a saturated aqueous sodium bicarbonate solution (50 mL), dried (sodium sulfate) and concentrated in vacuo to give a turbid brown oil as the crude product. The crude product was purified by column chromatography (silica gel, 100 g, eluted with ethyl acetate/n-heptane, gradient 25: 75 to 40: 60) to give the title compound as a white solid (2.29 g, 92%) after drying in vacuo (50° C., 5 mbar). HPLC (Method LCMS_Gradient) t _R = 4.6 min. ¹ H NMR (CDCl ₃ , 400MHz): δ0.81-0.89 (m, 6H), 0.92-0.98 (m, 9H), 1.46 (s, 3H), 1.48-1.69 (m, 2 H), 1.54(s, 9H), 1.59(s, 3H), 1.70(s, 3H), 2.44(s, 3H), 2.50-2.60(m, 1H), 2.93 -3.02 (m, 1H), 3.49 (d, J = 15.0Hz, 1H), 3.95-4.02 (m, 2H), 4.39 (d, J = 15.3Hz, 1H ), 7.33 (d, J = 8.1Hz, 2H), 7.36 (d, J = 2.4Hz, 1H), 7.76 (d, J = 8.3Hz, 2H), 11.10 (br s, 1H, exch). MS(ES+)m/z 791.4&789.6[M+H, Br].

Int-14B：4-甲基苯磺酸3-(((1S，3R)-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5-((叔丁氧基羰基)氨基)-3，6，6-三甲基-1-氧代-3，6-二氢-2H-1，4-噻嗪-1-亚基)氨基)丙酯的合成Int-14B: Synthesis of 3-(((1S,3R)-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5-((tert-butoxycarbonyl)amino)-3,6,6-trimethyl-1-oxo-3,6-dihydro-2H-1,4-thiazin-1-ylidene)amino)propyl 4-methylbenzenesulfonate

步骤1：(1S，3R)-5-氨基-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((3-羟基丙基)亚氨基)-3，6，6-三甲基-3，6-二氢-2H-1，4-噻嗪1-氧化物(Int-12B)Step 1: (1S,3R)-5-amino-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((3-hydroxypropyl)imino)-3,6,6-trimethyl-3,6-dihydro-2H-1,4-thiazine 1-oxide (Int-12B)

将2-((S，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-(3-羟基丙基)丙基磺酰亚胺基)-2-甲基丙腈(Int-11B，970mg，1.81mmol)溶解在乙醇(20mL)中并且加入溴化铜(I)(275mg，1.92mmol)。将混合物加热至75-80℃并且在该温度搅拌1.5h。之后，将其冷却至5℃(冰浴)并且倒到盐水(10mL)和氨水(25％w/w，5mL)的混合物上。将得到的混合物用MTBE(60mL)和乙酸乙酯(2x 25mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩，得到黄色油状物，为粗产物。将粗产物通过柱色谱纯化(硅胶，100g，用乙酸乙酯/甲醇，梯度95∶5至70∶30洗脱)，在真空中干燥(55℃，5毫巴)后得到标题化合物，为白色泡沫(670mg，69％)。HPLC(方法7626L05)tR＝4.7min。2-((S,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-(3-hydroxypropyl)propylsulfonylimino)-2-methylpropionitrile (Int-11B, 970 mg, 1.81 mmol) was dissolved in ethanol (20 mL) and copper (I) bromide (275 mg, 1.92 mmol) was added. The mixture was heated to 75-80° C. and stirred at this temperature for 1.5 h. Afterwards, it was cooled to 5° C. (ice bath) and poured onto a mixture of brine (10 mL) and aqueous ammonia (25% w/w, 5 mL). The resulting mixture was extracted with MTBE (60 mL) and ethyl acetate (2 x 25 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo to give a yellow oil as the crude product. The crude product was purified by column chromatography (silica gel, 100 g, elution with ethyl acetate/methanol, gradient 95:5 to 70:30) to give, after drying in vacuo (55° C., 5 mbar), the title compound as a white foam (670 mg, 69%). HPLC (Method 7626L05) tR=4.7 min.

步骤2：((1S，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((3-羟基丙基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-13B)Step 2: tert-Butyl ((1S,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((3-hydroxypropyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-13B)

将(1S，3R)-5-氨基-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((3-羟基丙基)亚氨基)-3，6，6-三甲基-3，6-二氢-2H-1，4-噻嗪1-氧化物(Int-12B，670mg，1.25mmol)溶解在THF(8mL)和水(1.7mL)中并且加入Boc-酐(350mg，372μl，1.6mmol)，碳酸氢钠(135mg，1.61mmol)和4-二甲基氨基吡啶(8mg，65μmol)。将混合物在室温搅拌2h。之后，加入氨水(25％w/w，54mg，60μl，0.79mmol)并且将混合物搅拌另外30min。然后将反应混合物倒到水(15mL)上并用MTBE(1x 40mL，1x 20mL)萃取。将合并的萃取物用盐水(10mL)洗涤，干燥(硫酸钠)并在真空中浓缩(50℃，5毫巴)，得到标题化合物，为浅棕色胶质(770mg，97％产率)，其在不进一步纯化的情况下用于下一步骤。HPLC(方法7626L05)t_R＝8.6min。(1S,3R)-5-amino-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((3-hydroxypropyl)imino)-3,6,6-trimethyl-3,6-dihydro-2H-1,4-thiazine 1-oxide (Int-12B, 670 mg, 1.25 mmol) was dissolved in THF (8 mL) and water (1.7 mL) and Boc-anhydride (350 mg, 372 μl, 1.6 mmol), sodium bicarbonate (135 mg, 1.61 mmol) and 4-dimethylaminopyridine (8 mg, 65 μmol) were added. The mixture was stirred at room temperature for 2 h. Afterwards, aqueous ammonia (25% w/w, 54 mg, 60 μl, 0.79 mmol) was added and the mixture was stirred for another 30 min. The reaction mixture was then poured onto water (15 mL) and extracted with MTBE (1 x 40 mL, 1 x 20 mL). The combined extracts were washed with brine (10 mL), dried (sodium sulfate) and concentrated in vacuo (50° C., 5 mbar) to afford the title compound as a light brown gum (770 mg, 97% yield), which was used in the next step without further purification. HPLC (Method 7626L05) t _R =8.6 min.

步骤3：4-甲基苯磺酸3-(((1S，3R)-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5-((叔丁氧基羰基)氨基)-3，6，6-三甲基-1-氧代-3，6-二氢-2H-1，4-噻嗪-1-亚基)氨基)丙酯(Int-14B)Step 3: 3-(((1S,3R)-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5-((tert-butoxycarbonyl)amino)-3,6,6-trimethyl-1-oxo-3,6-dihydro-2H-1,4-thiazin-1-ylidene)amino)propyl 4-methylbenzenesulfonate (Int-14B)

将((1S，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((3-羟基丙基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-13B，1.22g，1.92mmol)溶解在二氯甲烷(12mL)中，将溶液冷却至0-5℃(冰浴)并且接着在该温度加入三乙胺(486mg，670μL，4.81mmol)，甲苯磺酰氯(560mg，2.94mmol)在二氯甲烷(6ml)中的溶液和4-二甲基氨基吡啶(15mg，123μmol)。将得到的澄清溶液在室温搅拌16h。然后，将反应混合物倒到饱和氯化铵水溶液(25mL)上，并且在相分离后，将水相用MTBE(1x 100mL，1x 50mL)萃取。将合并的萃取物用饱和碳酸氢钠水溶液(25mL)洗涤，干燥(硫酸钠)并在真空中浓缩，得到浑浊褐色油状物，为粗产物。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度25∶75至50∶50洗脱)纯化，在在真空中干燥(50℃，5毫巴)后，得到标题化合物，为浅棕色胶质(1.25g，83％产率)。HPLC(方法7626L05)tR＝11.1min。Tert-butyl ((1S,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((3-hydroxypropyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-13B, 1.22 g, 1.92 mmol) was dissolved in dichloromethane (12 mL), the solution was cooled to 0-5° C. (ice bath) and then triethylamine (486 mg, 670 μL, 4.81 mmol), a solution of tosyl chloride (560 mg, 2.94 mmol) in dichloromethane (6 ml) and 4-dimethylaminopyridine (15 mg, 123 μmol) were added at this temperature. The resulting clear solution was stirred at room temperature for 16 h. The product was then poured onto a saturated aqueous ammonium chloride solution (25 mL) and, after phase separation, extracted with MTBE (1 x 100 mL, 1 x 50 mL). The combined extracts were washed with a saturated aqueous sodium bicarbonate solution (25 mL), dried (sodium sulfate) and concentrated in a vacuum to give a turbid brown oily substance, which was a crude product. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 25: 75 to 50: 50), dried in a vacuum (50 ° C, 5 mbar), to give the title compound, which was a light brown colloid (1.25 g, 83% yield). HPLC (method 7626L05) tR=11.1 min.

Int-15AA和Int-15AB：((4aR，5R，9R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-15AA)和((4aS，5R，9R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-15AB)的合成Int-15AA and Int-15AB: Synthesis of tert-butyl ((4aR,5R,9R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-15AA) and tert-butyl ((4aS,5R,9R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-15AB)

将4-甲基苯磺酸3-(((1R，3R)-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5-((叔丁氧基羰基)氨基)-3，6，6-三甲基-1-氧代-3，6-二氢-2H-1，4-噻嗪-1-亚基)氨基)丙酯(Int-14A，2.28g，2.89mmol)溶解在THF(50mL)中并且将溶液冷却至＜-70℃(丙酮/干冰浴)。在该温度将LHM S在THF中的溶液(1.0M，7.6ml，7.6mmol)经10min加入。然后，将得到的澄清的黄色溶液加温至0-5℃(冰浴)并在该温度搅拌1.5h。将反应混合物倒到2M氯化铵水溶液(50mL)上并用MTBE(1x 150mL，1x 50mL)萃取。将合并的萃取物用盐水(30mL)洗涤，干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，100g，用乙酸乙酯/正庚烷，梯度30∶70至60∶40洗脱)纯化，在真空中干燥(55℃，5毫巴)后，得到标题化合物，为分离的非对映异构体。分离首先洗脱的非对映异构体(Int-15AA)，为白色泡沫(800mg，45％)，并且获得其次洗脱的非对映异构体(Int-15AB)，为白色泡沫(670mg，37％)。3-(((1R,3R)-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5-((tert-butoxycarbonyl)amino)-3,6,6-trimethyl-1-oxo-3,6-dihydro-2H-1,4-thiazin-1-ylidene)amino)propyl 4-methylbenzenesulfonate (Int-14A, 2.28 g, 2.89 mmol) was dissolved in THF (50 mL) and the solution was cooled to <-70° C. (acetone/dry ice bath). At this temperature, a solution of LHMS in THF (1.0 M, 7.6 ml, 7.6 mmol) was added over 10 min. The resulting clear yellow solution was then warmed to 0-5° C. (ice bath) and stirred at this temperature for 1.5 h. Reaction mixture is poured onto 2M aqueous ammonium chloride solution (50mL) and extracted with MTBE (1x 150mL, 1x 50mL).The extracts merged are washed with salt water (30mL), dried (sodium sulfate) and concentrated in a vacuum.The crude product is passed through column chromatography (silica gel, 100g, eluted with ethyl acetate/normal heptane, gradient 30: 70 to 60: 40) purifying, after drying (55 ℃, 5 mbar) in a vacuum, obtain title compound, be the diastereomer of separation.Separate the diastereomer (Int-15AA) that first washes out, be white foam (800mg, 45%), and obtain the diastereomer (Int-15AB) that second washes out, be white foam (670mg, 37%).

Int-15AA：HPLC(方法LCMS_梯度)t_R＝4.4min。¹H NMR(CDCl₃，400MHz)：δ0.82-0.91(m，6H)，0.94-1.00(m，9H)，1.48(s，9H)，1.77(s，3H)，1.79(s，3H)，1.81-2.01(m，2H)，1.91(d，J＝1.3Hz，3H)，2.07-2.14(m，1H)，2.38(dddd，J＝3.0，12.9，13.2，13.2Hz，1H)，3.36-3.44(m，1H)，3.62(ddd，J＝3.5，12.7，12.7Hz，1H)，4.17(dd，J＝3.4，12.5Hz，1H)，7.42(d，J＝2.4Hz，1H)，11.37(br s，1H，exch)。MS(ES+)m/z 619.3&617.6[M+H，Br]。Int-15AA: HPLC (method LCMS_gradient) t _R =4.4 min. ¹ H NMR (CDCl ₃ , 400 MHz): δ 0.82-0.91 (m, 6H), 0.94-1.00 (m, 9H), 1.48 (s, 9H), 1.77 (s, 3H), 1.79 (s, 3H), 1.81-2.01 (m, 2H), 1.91 (d, J=1.3 Hz, 3H), 2.07-2.14 (m, 1H), 2.38 ( dddd, J=3.0, 12.9, 13.2, 13.2Hz, 1H), 3.36-3.44 (m, 1H), 3.62 (dddd, J=3.5, 12 .7, 12.7Hz, 1H), 4.17 (dd, J=3.4, 12.5Hz, 1H), 7.42 (d, J=2.4Hz, 1H), 11.37 (br s, 1H, exch). MS(ES+)m/z 619.3&617.6[M+H, Br].

Int-15AB：HPLC(方法LCMS_梯度)t_R＝4.2min。¹H NMR(CDCl₃，400MHz)：δ0.83-0.91(m，6H)，0.93-0.99(m，9H)，1.49-1.69(m，2H)，1.59(s，9H)，1.71-1.82(m，2H)，1.78(s，3H)，1.89(s，3H)，2.02(d，J＝0.8Hz，3H)，3.37-3.47(m，1H)，3.56-3.70(m，2H)，7.45(d，J＝2.7Hz，1H)，11.82(br s，1H，exch)。MS(ES+)m/z 619.3&617.6[M+H，Br]。Int-15AB: HPLC (method LCMS_gradient) t _R =4.2 min. ¹ H NMR (CDCl ₃ , 400 MHz): δ 0.83-0.91 (m, 6H), 0.93-0.99 (m, 9H), 1.49-1.69 (m, 2H), 1.59 (s, 9H), 1.71-1.82 (m, 2H), 1.78 (s, 3H), 1.89 (s, 3H), 2.02 (d, J=0.8 Hz, 3H), 3.37-3.47 (m, 1H), 3.56-3.70 (m, 2H), 7.45 (d, J=2.7 Hz, 1H), 11.82 (br s, 1H, exch). MS(ES+)m/z 619.3&617.6[M+H, Br].

Int-15BA和Int-15BB：((4aR，5R，9S)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-15BA)和((4aS，5R，9S)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-15BB)的合成Int-15BA and Int-15BB: Synthesis of tert-butyl ((4aR,5R,9S)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-15BA) and tert-butyl ((4aS,5R,9S)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-15BB)

将4-甲基苯磺酸3-(((1S，3R)-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5-((叔丁氧基羰基)氨基)-3，6，6-三甲基-1-氧代-3，6-二氢-2H-1，4-噻嗪-1-亚基)氨基)丙酯(Int-14B，1.25g，1.58mmol)溶解在THF(28mL)中并且将溶液冷却至＜-70℃(丙酮/干冰浴)。在该温度经10min加入LHMDS在THF(1.0M，4.2ml，4.2mmol)中的溶液。然后，将得到的澄清的黄色溶液加温至0-5℃(冰浴)并在该温度搅拌2.5h。将反应混合物倒到2M氯化铵水溶液(50mL)上并用MTBE(1x 100mL，1x 50mL)萃取。将合并的萃取物用盐水(30mL)洗涤，干燥(硫酸钠)并在真空中浓缩，得到黄色泡沫，为粗产物(1.06g)。粗产物以约7∶3的比例含有两种非对映异构体(Int-15BA/Int-15BB，通过HPLC)并且在不进一步纯化的情况下用于下一步骤。3-(((1S,3R)-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5-((tert-butoxycarbonyl)amino)-3,6,6-trimethyl-1-oxo-3,6-dihydro-2H-1,4-thiazin-1-ylidene)amino)propyl 4-methylbenzenesulfonate (Int-14B, 1.25 g, 1.58 mmol) was dissolved in THF (28 mL) and the solution was cooled to <-70° C. (acetone/dry ice bath). A solution of LHMDS in THF (1.0 M, 4.2 ml, 4.2 mmol) was added at this temperature over 10 min. The resulting clear yellow solution was then warmed to 0-5° C. (ice bath) and stirred at this temperature for 2.5 h. The reaction mixture was poured onto 2 M aqueous ammonium chloride solution (50 mL) and extracted with MTBE (1 x 100 mL, 1 x 50 mL). The combined extracts were washed with brine (30 mL), dried (sodium sulfate) and concentrated in vacuo to give a yellow foam as a crude product (1.06 g). The crude product contained two diastereomers (Int-15BA/Int-15BB by HPLC) in a ratio of approximately 7:3 and was used in the next step without further purification.

Int-15BA：HPLC(方法LCMS_梯度)t_R＝4.1min。MS(ES+)m/z 619.3&617.3[M+H，Br]。Int-15BA: HPLC (method LCMS_gradient) _tR = 4.1 min. MS (ES+) m/z 619.3 & 617.3 [M+H, Br].

Int-15BB：HPLC(方法LCMS_梯度)t_R＝4.3min。MS(ES+)m/z 619.3&617.3[M+H，Br]。Int-15BB: HPLC (method LCMS_gradient) t _R =4.3 min. MS (ES+) m/z 619.3 & 617.3 [M+H, Br].

Int-17AA：((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯的合成Int-17AA: Synthesis of tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate

步骤1：((4aR，5R，9R)-5-(6-溴-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-16AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-bromo-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-16AA)

将((4aR，5R，9R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-15AA，780mg，1.26mmol)溶解在THF(9.6mL)和DMF(2.4mL)中。在室温加入乙酸(157mg，150μl，2.62mmol)和氟化钾(150mg，2.58mmol)并且将得到的精细(fine)悬浮液在该温度搅拌2h。之后，将其倒到饱和碳酸氢钠水溶液(20mL)上并用MTBE(1x 80mL，1x 40mL)萃取。将合并的萃取物用盐水(20mL)洗涤，干燥(硫酸钠)并在真空中浓缩，得到白色泡沫，为粗产物(650mg)。通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度60∶40至80∶20洗脱)纯化粗产物，在真空中干燥(50℃，5毫巴)后，得到标题化合物，为白色固体(550mg，86％)。HPLC(方法LCMS_梯度)t_R＝2.8min。¹H NMR(CDCl₃，400MHz)：δ1.48(s，9H)，1.77(s，3H)，1.81(s，3H)，1.82-1.95(m，2H)，1.92(d，J＝1.6Hz，3H)，1.95-2.05(m，1H)，2.38(dddd，J＝3.5，12.4，12.6，13.4Hz，1H)，3.36-3.43(m，1H)，3.61(ddd，J＝3.5，12.5，12.5Hz，1H)，4.08-4.14(m，1H)，7.38(dd，J＝8.3，10.8Hz，1H)，7.51(dd，J＝3.1，8.5Hz，1H)，11.23(br s，1H，exch)。MS(ES+)m/z 505.3&503.4[M+H，Br]。Tert-butyl ((4aR, 5R, 9R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-15AA, 780 mg, 1.26 mmol) was dissolved in THF (9.6 mL) and DMF (2.4 mL). Acetic acid (157 mg, 150 μl, 2.62 mmol) and potassium fluoride (150 mg, 2.58 mmol) were added at room temperature and the resulting fine suspension was stirred at this temperature for 2 h. Afterwards, it was poured onto saturated aqueous sodium bicarbonate solution (20 mL) and extracted with MTBE (1 x 80 mL, 1 x 40 mL). The combined extracts were washed with brine (20 mL), dried (sodium sulfate) and concentrated in vacuo to give a white foam as a crude product (650 mg). The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 60:40 to 80:20) to give the title compound as a white solid (550 mg, 86%) after drying in vacuo (50° C., 5 mbar). HPLC (method LCMS_Gradient) t _R = 2.8 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.48 (s, 9H), 1.77 (s, 3H), 1.81 (s, 3H), 1.82-1.95 (m, 2H), 1.9 2(d, J=1.6Hz, 3H), 1.95-2.05 (m, 1H), 2.38 (dddd, J=3.5, 12.4, 12.6, 13.4H z, 1H), 3.36-3.43 (m, 1H), 3.61 (ddd, J=3.5, 12.5, 12.5Hz, 1H), 4.08-4.14 ( m, 1H), 7.38 (dd, J=8.3, 10.8Hz, 1H), 7.51 (dd, J=3.1, 8.5Hz, 1H), 11.23 (br s, 1H, exch). MS(ES+)m/z 505.3&503.4[M+H, Br].

步骤2：((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-17AA)Step 2: tert-Butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-17AA)

将((4aR，5R，9R)-5-(6-溴-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-16AA，300mg，596μmol)溶解在1，4-二烷(5mL)和水(1.7mL)中并且加入叠氮化钠(400mg，6.15mmol)。接着加入碘化酮(I)(63mg，331μmol)，抗坏血酸钠(33mg，167μmol)和反式-N，N′-二甲基-1，2-环己烷二胺(72.2mg，80μl，507μmol)并且将深蓝色混合物在70℃搅拌1h。然后，将反应混合物冷却至室温并且倒到饱和碳酸氢钠水溶液(20mL)上。将其用乙酸乙酯(1x 60mL，1x 30mL)萃取，将合并的萃取物经硫酸钠干燥并且将硅胶(2g)加入至溶液中。过滤后，将滤液在真空中浓缩，得到绿色固体，为粗产物。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度80∶20至100∶0洗脱)纯化，在真空中干燥(50℃，5毫巴)后得到标题化合物，为灰白色固体(150mg，57％)。HPLC(方法LCMS_梯度)t_R＝2.2min。¹H NMR(CDCl₃，400MHz)：δ1.48(s，9H)，1.76(s，3H)，1.79-1.95(m，2H)，1.82(s，3H)，1.87(d，J＝1.9Hz，3H)，2.01-2.08(m，1H)，2.35(dddd，J＝3.8，12.6，13.2，13.2Hz，1H)，3.34-3.42(m，1H)，3.61(ddd，J＝3.9，12.4，12.4Hz，1H)，4.09-4.16(m，1H)，4.38(s，2H)，6.47(dd，J＝2.4，8.9Hz，1H)，7.24(dd，J＝8.7，11.1Hz，1H)，11.12(br s，1H，exch)。MS(ES+)m/z 440.3[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-bromo-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-16AA, 300 mg, 596 μmol) was dissolved in 1,4-dioxane (5 mL) and water (1.7 mL) and sodium azide (400 mg, 6.15 mmol) was added. Iodide ketone (I) (63 mg, 331 μmol), sodium ascorbate (33 mg, 167 μmol) and trans-N,N′-dimethyl-1,2-cyclohexanediamine (72.2 mg, 80 μl, 507 μmol) were then added and the dark blue mixture was stirred at 70° C. for 1 h. Then, the reaction mixture was cooled to room temperature and poured onto a saturated aqueous sodium bicarbonate solution (20 mL). It was extracted with ethyl acetate (1 x 60 mL, 1 x 30 mL), the combined extracts were dried over sodium sulfate and silica gel (2 g) was added to the solution. After filtration, the filtrate was concentrated in vacuo to give a green solid as a crude product. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 80: 20 to 100: 0) and dried in vacuo (50 ° C, 5 mbar) to give the title compound as an off-white solid (150 mg, 57%). HPLC (method LCMS_gradient) t _R = 2.2 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.48 (s, 9H), 1.76 (s, 3H), 1.79-1.95 (m, 2H), 1.82 (s, 3H), 1.87 (d , J=1.9Hz, 3H), 2.01-2.08 (m, 1H), 2.35 (dddd, J=3.8, 12.6, 13.2, 13.2Hz, 1H), 3.34-3.42 (m, 1H), 3.61 (ddd, J=3.9, 12.4, 12.4Hz, 1H), 4.09-4.16 (m, 1H), 4. 38 (s, 2H), 6.47 (dd, J=2.4, 8.9Hz, 1H), 7.24 (dd, J=8.7, 11.1Hz, 1H), 11.12 (br s, 1H, exch). MS(ES+)m/z 440.3[M+H].

Int-17AB：((4aS，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯的合成Int-17AB: Synthesis of tert-butyl ((4aS,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate

步骤1：((4aS，5R，9R)-5-(6-溴-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-16AB)Step 1: tert-Butyl ((4aS,5R,9R)-5-(6-bromo-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-16AB)

将((4aS，5R，9R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-15AB，650mg，1.05mmol)溶解在THF(8mL)和DMF(2mL)中。在室温加入乙酸(131mg，125μl，2.18mmol)和氟化钾(125mg，2.15mmol)并且将得到的精细悬浮液在该温度搅拌2h。之后，将其倒到饱和碳酸氢钠水溶液(20mL)上并用MTBE(1x60mL，1x30mL)萃取。将合并的萃取物用盐水(15mL)洗涤，干燥(硫酸钠)并在真空中浓缩，得到白色固体，为粗产物。将粗产物悬浮在MTBE(10mL)中并且在室温搅拌10min。加入正庚烷(10mL)，过滤沉淀，用正庚烷(10mL)洗涤并在真空中干燥(50℃，5毫巴)，得到标题化合物，为白色粉末(410mg，77％)。HPLC(方法LCMS_梯度)t_R＝2.6min。¹H NMR(CDCl₃，400MHz)：δ1.58(s，9H)，1.59-1.70(m，2H)，1.72-1.82(m，2H)，1.79(s，3H)，1.90(s，3H)，2.03(d，J＝1.1Hz，3H)，3.38-3.48(m，1H)，3.54-3.61(m，1H)，3.63-3.70(m，1H)，7.39(dd，J＝8.5，10.5Hz，1H)，7.55(dd，J＝3.1，8.5Hz，1H)，11.86(br s，1H，exch)。MS(ES+)m/z 505.3&503.4[M+H，Br]。Tert-butyl ((4aS, 5R, 9R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a, 5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-15AB, 650 mg, 1.05 mmol) was dissolved in THF (8 mL) and DMF (2 mL). Acetic acid (131 mg, 125 μl, 2.18 mmol) and potassium fluoride (125 mg, 2.15 mmol) were added at room temperature and the resulting fine suspension was stirred at this temperature for 2 h. Afterwards, it was poured onto a saturated aqueous sodium bicarbonate solution (20 mL) and extracted with MTBE (1x60 mL, 1x30 mL). The combined extracts were washed with brine (15 mL), dried (sodium sulfate) and concentrated in vacuo to give a white solid as a crude product. The crude product was suspended in MTBE (10 mL) and stirred at room temperature for 10 min. n-heptane (10 mL) was added, the precipitate was filtered, washed with n-heptane (10 mL) and dried in vacuo (50° C., 5 mbar) to give the title compound as a white powder (410 mg, 77%). HPLC (method LCMS_gradient) t _R = 2.6 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.58 (s, 9H), 1.59-1.70 (m, 2H), 1.72-1.82 (m, 2H), 1.79 (s, 3H), 1.90 (s, 3H), 2.03 (d, J=1.1Hz, 3H), 3.38- 3.48 (m, 1H), 3.54-3.61 (m, 1H), 3.63-3.70 (m, 1H), 7.39 (dd, J=8.5, 10.5Hz, 1H), 7.55 (dd, J=3.1, 8.5Hz, 1H), 11.86 (br s, 1H, exch). MS(ES+)m/z 505.3&503.4[M+H, Br].

步骤2：((4aS，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-17AB)Step 2: tert-Butyl ((4aS,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-17AB)

将((4aS，5R，9R)-5-(6-溴-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-16AB，180mg，358μmol)溶解在1，4-二烷(3mL)和水(1mL)中并且加入叠氮化钠(240mg，3.69mmol)。接着加入碘化酮(I)(38mg，200μmol)，抗坏血酸钠(20mg，101μmol)和反式-N，N′-二甲基-1，2-环己烷二胺(45.1mg，50μl，317μmol)并且将深蓝色混合物在70℃搅拌1h。之后，加入第二部分叠氮化钠(120mg，1.85mmol)，以及碘化酮(I)(38mg，200μmol)，抗坏血酸钠(20mg，101μmol)和反式-N，N′-二甲基-1，2-环己烷二胺(45.1mg，50μl，317μmol)在二烷(0.5mL)中的悬浮液并且将混合物在70℃搅拌另外45min。然后，将反应混合物冷却至室温并且倒到饱和碳酸氢钠水溶液(15mL)上。将其用乙酸乙酯(1x60mL，1x30mL)萃取，将合并的萃取物经硫酸钠干燥并且将硅胶(2g)加入至溶液中。过滤后，将滤液在真空中浓缩，得到绿色粘性固体，为粗产物(200mg)。将粗产物通过柱色谱(硅胶，50g，用二氯甲烷/甲醇，梯度99∶1至95∶5洗脱)纯化，在真空中干燥(50℃，5毫巴)后得到标题化合物，为灰白色固体(140mg，89％)。HPLC(方法LCMS_梯度)t_R＝1.8min。¹H NMR(CDCl₃，400MHz)：δ1.56(s，9H)，1.62-1.84(m，4H)，1.79(s，3H)，1.89(s，3H)，1.98(d，J＝0.8Hz，3H)，3.35-3.45(m，1H)，3.56-3.69(m，2H)，4.57(s，2H)，6.48(dd，J＝2.4，8.9Hz，1H)，7.26(dd，J＝8.6，10.5Hz，1H)，12.07(br s，1H，exch)。MS(ES+)m/z 440.3[M+H]。Tert-butyl ((4aS,5R,9R)-5-(6-bromo-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-16AB, 180 mg, 358 μmol) was dissolved in 1,4-dioxane (3 mL) and water (1 mL) and sodium azide (240 mg, 3.69 mmol) was added. Iodide ketone (I) (38 mg, 200 μmol), sodium ascorbate (20 mg, 101 μmol) and trans-N,N′-dimethyl-1,2-cyclohexanediamine (45.1 mg, 50 μl, 317 μmol) were then added and the dark blue mixture was stirred at 70° C. for 1 h. Afterwards, add second portion sodium azide (120mg, 1.85mmol), and iodine ketone (I) (38mg, 200μmol), sodium ascorbate (20mg, 101μmol) and trans-N, N '-dimethyl-1, the suspension of 2-cyclohexanediamine (45.1mg, 50μl, 317μmol) in dioxane (0.5mL) and mixture is stirred at 70 ℃ for other 45min.Then, reaction mixture is cooled to room temperature and poured on saturated sodium bicarbonate aqueous solution (15mL).It is extracted with ethyl acetate (1x60mL, 1x30mL), the extract merged is dried over sodium sulfate and silica gel (2g) is added in the solution.After filtration, filtrate is concentrated in a vacuum, obtains green sticky solid, is crude product (200mg). The crude product was purified by column chromatography (silica gel, 50 g, elution with dichloromethane/methanol, gradient 99:1 to 95:5) to afford after drying in vacuo (50° C., 5 mbar) the title compound as an off-white solid (140 mg, 89%). HPLC (method LCMS_Gradient) t _R =1.8 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.56 (s, 9H), 1.62-1.84 (m, 4H), 1.79 (s, 3H), 1.89 (s, 3H), 1.98 (d, J=0.8Hz, 3H), 3.35-3.45 (m , 1H), 3.56-3.69 (m, 2H), 4.57 (s, 2H), 6.48 (dd, J=2.4, 8.9Hz, 1H), 7.26 (dd, J=8.6, 10.5Hz, 1H), 12.07 (br s, 1H, exch). MS(ES+)m/z 440.3[M+H].

Int-17BA：((4aR，5R，9S)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯的合成Int-17BA: Synthesis of tert-butyl ((4aR,5R,9S)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate

步骤1：((4aR，5R，9S)-5-(6-溴-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-16BA)Step 1: tert-Butyl ((4aR,5R,9S)-5-(6-bromo-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-16BA)

将((4aR，5R，9S)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯和((4aS，5R，9S)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-15BA/Int-15BB约7∶3，1.00g，1.62mmol)的混合物溶解在THF(12mL)和DMF(3mL)中。在室温加入乙酸(315mg，300μl，5.24mmol)和氟化钾(300mg，5.16mmol)并将得到的精细悬浮液在该温度搅拌2h。之后，将其倒到饱和碳酸氢钠水溶液(25mL)上并用MTBE(1x100mL，1x50mL)萃取。将合并的萃取物用盐水(25mL)洗涤，干燥(硫酸钠)并在真空中浓缩，得到黄色粘性油状物，为粗产物。通过HPLC和tlc的反应控制表明，仅Int-15BA转化为脱硅的(desilated)产物，另一非对映异构体(Int-15BB)不反应。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度60∶40至80∶20洗脱)纯化，在真空中干燥(50℃，5毫巴)后，得到脱硅的化合物(Int-16BA)，为白色泡沫(460mg，56％产率)。还将剩余起始材料分离为纯的Int-15BB，为黄色油状物(210mg，21％)。tert-Butyl ((4aR,5R,9S)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate and ((4aS,5R,9S)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate A mixture of tert-butyl methyl-1-[1-[[1-[1-[1-[1-thiazolyl]-1-[1-[1-[1-thiazolyl]-1-[1-[1-[1-thiazolyl]-1-[1-[1-thiazolyl]-1-[1-[1-thiazolyl]-1-[1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1-[1-thiazolyl]-1- Reaction control by HPLC and tlc showed that only Int-15BA was converted into a desiliconized product, while the other diastereomer (Int-15BB) did not react. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 60: 40 to 80: 20) and dried in vacuo (50° C., 5 mbar) to give the desiliconized compound (Int-16BA) as a white foam (460 mg, 56% yield). The remaining starting material was also isolated as pure Int-15BB as a yellow oil (210 mg, 21%).

Int-16BA：HPLC(方法LCMS_梯度)t_R＝2.6min。¹H NMR(CDCl₃，400MHz)：δ1.44(s，3H)，1.55(s，9H)，1.77(s，3H)，1.85(s，3H)，1.87-1.95(m，2H)，2.12-2.24(m，1H)，2.51-2.59(m，1H)，3.49-3.57(m，1H)，3.63-3.72(m，2H)，7.25(dd，J＝8.5，10.6Hz，1H)，7.40(dd，J＝3.1，8.5Hz，1H)，10.69(br s，1H，exch)。MS(ES+)m/z 505.3&503.4[M+H，Br]。Int-16BA: HPLC (method LCMS_gradient) t _R =2.6 min. ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.44 (s, 3H), 1.55 (s, 9H), 1.77 (s, 3H), 1.85 (s, 3H), 1.87-1.95 (m, 2H), 2.12-2.24 (m, 1H), 2.51-2.59 (m, 1H), 3.49-3.57 (m, 1H), 3.63-3.72 (m, 2H), 7.25 (dd, J=8.5, 10.6 Hz, 1H), 7.40 (dd, J=3.1, 8.5 Hz, 1H), 10.69 (br s, 1H, exch). MS(ES+)m/z 505.3&503.4[M+H, Br].

步骤2：((4aR，5R，9S)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-17BA)Step 2: tert-Butyl ((4aR,5R,9S)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-17BA)

将((4aR，5R，9S)-5-(6-溴-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-16BA，450mg，894μmol)溶解在1，4-二烷(7.5mL)和水(2.5mL)中并且加入叠氮化钠(600mg，9.23mmol)。接着加入碘化酮(I)(90mg，473μmol)，抗坏血酸钠(45mg，227μmol)和反式-N，N′-二甲基-1，2-环己烷二胺(108mg，120μl，761μmol)并且将深蓝色混合物在70℃搅拌1h。之后，加入第二部分叠氮化钠(200mg，3.08mmol)，碘化酮(I)(45mg，236μmol)，抗坏血酸钠(25mg，126μmol)和反式-N，N′-二甲基-1，2-环己烷二胺(60μl，380μmol)并将混合物在70℃搅拌另外20min。然后，让反应混合物冷却至室温并且倒到饱和碳酸氢钠水溶液(25mL)上。将其用乙酸乙酯(1x100mL，1x50mL)萃取，将合并的萃取物经硫酸钠干燥并且将硅胶(4g)加入至溶液中。过滤后，将滤液在真空中浓缩，得到绿色粘性固体，为粗产物。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度80∶20至100∶0洗脱)纯化，在真空中干燥(50℃，5毫巴)后，得到标题化合物，为白色固体(290mg，74％)。HPLC(方法LCMS_梯度)t_R＝2.0min。¹H NMR(CDCl₃，400MHz)：δ1.34(s，3H)，1.54(s，9H)，1.73(s，3H)，1.79(s，3H)，1.81-1.92(m，2H)，2.15-2.26(m，1H)，2.47-2.57(m，1H)，3.40(ddd，J＝5.0，8.6，13.3Hz，1H)，3.64(ddd，J＝4.8，4.8，13.2Hz，1H)，3.98(dd，J＝4.8，10.2Hz，1H)，4.31(s，2H)，6.38(dd，J＝2.4，8.6Hz，1H)，7.15(dd，J＝8.7，10.9Hz，1H)，10.47(br s，1H，exch)。MS(ES+)m/z 440.4[M+H]。Tert-butyl ((4aR,5R,9S)-5-(6-bromo-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-16BA, 450 mg, 894 μmol) was dissolved in 1,4-dioxane (7.5 mL) and water (2.5 mL) and sodium azide (600 mg, 9.23 mmol) was added. Iodide ketone (I) (90 mg, 473 μmol), sodium ascorbate (45 mg, 227 μmol) and trans-N,N′-dimethyl-1,2-cyclohexanediamine (108 mg, 120 μl, 761 μmol) were then added and the dark blue mixture was stirred at 70° C. for 1 h. Afterwards, add second portion sodium azide (200mg, 3.08mmol), iodine ketone (I) (45mg, 236μmol), sodium ascorbate (25mg, 126μmol) and trans-N, N '-dimethyl-1,2-cyclohexanediamine (60 μl, 380μmol) and stir mixture at 70 ℃ for other 20min.Then, allow reaction mixture to cool to room temperature and pour on saturated sodium bicarbonate aqueous solution (25mL).It is extracted with ethyl acetate (1x100mL, 1x50mL), the extract merged is dried over sodium sulfate and silica gel (4g) is added in solution.After filtration, filtrate is concentrated in a vacuum, obtains green sticky solid, is crude product. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 80:20 to 100:0) to give, after drying in vacuo (50° C., 5 mbar), the title compound as a white solid (290 mg, 74%). HPLC (method LCMS_gradient) t _R = 2.0 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.34 (s, 3H), 1.54 (s, 9H), 1.73 (s, 3H), 1.79 (s, 3H), 1.81-1.92 (m, 2H), 2.15-2.26 (m, 1H), 2.47-2.57 (m, 1H), 3.40 (ddd, J=5.0, 8.6, 13.3Hz , 1H), 3.64 (ddd, J=4.8, 4.8, 13.2Hz, 1H), 3.98 (dd, J=4.8, 10.2Hz, 1H), 4.31 (s, 2H), 6.38 (dd, J=2.4, 8.6Hz, 1H), 7.15 (dd, J=8.7, 10.9Hz, 1H), 10.47 (br s, 1H, exch). MS(ES+)m/z 440.4[M+H].

Int-9：(R，E)-N-(1-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)亚乙基)-2-甲基丙-2-亚磺酰胺的合成Int-9: Synthesis of (R,E)-N-(1-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)ethylidene)-2-methylpropane-2-sulfenamide

步骤1：2-溴-5-氟-4-(三乙基甲硅烷基)吡啶(Int-20)Step 1: 2-Bromo-5-fluoro-4-(triethylsilyl)pyridine (Int-20)

在-20℃向二异丙胺(130g，183mL，1.28mol)在四氢呋喃(1500mL)中的溶液中加入在四氢呋喃中的1.6M正丁基锂(800mL，1.28mol)。将反应混合物加温至0℃并且搅拌另外30分钟。在-70℃加入由四氢呋喃(200mL)中的2-溴-5-氟吡啶(Int-19，205g，1.16mol)组成的溶液。在60分钟后，经30分钟逐滴加入三乙基氯硅烷(193g，217mL，1.28mol)。在-70℃继续搅拌1h并且然后将其加温至-30℃。将反应混合物倒到1M氯化氢水溶液(1000mL)和13-％氯化铵水溶液的混合物上。分离层。将水层用叔丁基甲醚(2000mL)萃取。将合并的有机层用一个1500-mL部分的水洗涤并在真空中浓缩，得到粗制标题化合物(345g，定量的)，为橙色油状物，在不进一步纯化的情况下将其用于下一步骤。MS(ES+)m/z 290.1&292.1[M+H，Br]。To a solution of diisopropylamine (130g, 183mL, 1.28mol) in tetrahydrofuran (1500mL) was added 1.6M n-butyllithium (800mL, 1.28mol) in tetrahydrofuran at -20°C. The reaction mixture was heated to 0°C and stirred for another 30 minutes. A solution consisting of 2-bromo-5-fluoropyridine (Int-19, 205g, 1.16mol) in tetrahydrofuran (200mL) was added at -70°C. After 60 minutes, triethylchlorosilane (193g, 217mL, 1.28mol) was added dropwise over 30 minutes. Stirring was continued at -70°C for 1h and then heated to -30°C. The reaction mixture was poured onto a mixture of 1M aqueous hydrogen chloride solution (1000mL) and 13% aqueous ammonium chloride solution. The layers were separated. The aqueous layer was extracted with tert-butyl methyl ether (2000mL). The combined organic layers were washed with one 1500-mL portion of water and concentrated in vacuo to give the crude title compound (345 g, quantitative) as an orange oil, which was used in the next step without further purification. MS (ES+) m/z 290.1 & 292.1 [M+H, Br].

步骤2：1-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)乙酮(Int-21)Step 2: 1-(6-Bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)ethanone (Int-21)

在-20℃向二异丙胺(160g，225mL，1.55mol)在四氢呋喃(2200mL)中的溶液中加入四氢呋喃中的1.6M正丁基锂(950mL，1.52mol)。将反应混合物加温至0℃并且搅拌另外30分钟。在-70℃经30分钟逐滴加入2-溴-5-氟-4-(三乙基甲硅烷基)吡啶(338g，1.16mol)在四氢呋喃(300mL)中的溶液。在80分钟后，经10分钟逐滴加入N，N-二甲基乙酰胺(107g，115mL，1.22mol)。去除冷浴并且将反应混合物倒到25-％氯化氢水溶液(255g，227mL，1.75mol)，10-％氯化钠水溶液(2500mL)的混合物上。分层。将水层用叔丁基甲醚(2500mL)萃取。将合并的有机层在真空中浓缩，得到粗制标题化合物(392g，定量的)，为深棕色粘性油状物，在不进一步纯化的情况下将其用于下一步骤。MS(ES+)m/z 332.1&334.1[M+H，Br]。To a solution of diisopropylamine (160 g, 225 mL, 1.55 mol) in tetrahydrofuran (2200 mL) was added 1.6 M n-butyllithium (950 mL, 1.52 mol) in tetrahydrofuran at -20 ° C. The reaction mixture was warmed to 0 ° C and stirred for another 30 minutes. A solution of 2-bromo-5-fluoro-4-(triethylsilyl)pyridine (338 g, 1.16 mol) in tetrahydrofuran (300 mL) was added dropwise at -70 ° C over 30 minutes. After 80 minutes, N, N-dimethylacetamide (107 g, 115 mL, 1.22 mol) was added dropwise over 10 minutes. The cooling bath was removed and the reaction mixture was poured onto a mixture of 25% aqueous hydrogen chloride solution (255 g, 227 mL, 1.75 mol) and 10% aqueous sodium chloride solution (2500 mL). The layers were separated. The aqueous layer was extracted with tert-butyl methyl ether (2500 mL). The combined organic layers were concentrated in vacuo to give the crude title compound (392 g, quantitative) as a dark brown viscous oil, which was used in the next step without further purification. MS (ES+) m/z 332.1 & 334.1 [M+H, Br].

步骤3：(R，E)-N-(1-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)亚乙基)-2-甲基丙-2-亚磺酰胺(Int-9)Step 3: (R,E)-N-(1-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)ethylidene)-2-methylpropane-2-sulfenamide (Int-9)

向1-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)乙酮(200g，512mmol)和(R)-2-甲基丙-2-亚磺酰胺(77.5g，640mmol)在乙酸乙酯(2000mL)中的混合物中加入乙醇钛(IV)(187g，171mL，819mmol)。将反应混合物在60℃加热并搅拌过夜。去除热浴并且通过在40℃加入水(24.0g，24mL，1.33mol)将过量的乙醇钛(IV)猝灭。通过过滤移除固体并用两个500-mL部分的水洗涤。将滤液用一个1000-mL部分的5-％氯化氢水溶液和一个5-％碳酸氢钠水溶液洗涤。将有机层经无水硫酸钠干燥，过滤并在真空中浓缩。通过急骤色谱，利用正庚烷/乙酸乙酯作为洗脱剂纯化，得到标题化合物，为棕色粘性油状物。MS(ES+)m/z 435.2&437.2[M+H，Br]。To a mixture of 1-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)ethanone (200 g, 512 mmol) and (R)-2-methylpropane-2-sulfinamide (77.5 g, 640 mmol) in ethyl acetate (2000 mL) was added titanium (IV) ethoxide (187 g, 171 mL, 819 mmol). The reaction mixture was heated at 60 ° C and stirred overnight. The hot bath was removed and the excess titanium (IV) ethoxide was quenched by adding water (24.0 g, 24 mL, 1.33 mol) at 40 ° C. The solid was removed by filtration and washed with two 500-mL portions of water. The filtrate was washed with a 1000-mL portion of 5-% aqueous hydrogen chloride solution and a 5-% aqueous sodium bicarbonate solution. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated in vacuo. Purification by flash chromatography using n-heptane/ethyl acetate as eluent gave the title compound as a brown viscous oil. MS(ES+)m/z 435.2&437.2[M+H, Br].

Int-24BB：((4aS，5R，9S)-5-(6-氨基-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯的合成Int-24BB: Synthesis of tert-butyl ((4aS,5R,9S)-5-(6-amino-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate

将((4aS，5R，9S)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-15BB，210mg，340μmol)溶解在1，4-二烷(4mL)和水(1mL)中并且加入叠氮化钠(225mg，3.46mmol)。接着加入碘化酮(I)(35mg，184μmol)，抗坏血酸钠(17.5mg，88.3μmol)和反式-N，N′-二甲基-1，2-环己烷二胺(45.1mg，50μl，317μmol)并且将深蓝色混合物在70℃搅拌1h。之后，将其冷却至室温并且加入第二部分的叠氮化钠(225mg，3.46mmol)，碘化酮(I)(40mg，210μmol)，抗坏血酸钠(20mg，101μmol)和反式-N，N′-二甲基-1，2-环己烷二胺(60μl，380μmol)并且将混合物在70℃搅拌另外1h min。之后，将其冷却至室温并且加入第三部分的叠氮化钠(225mg，3.46mmol)，碘化酮(I)(40mg，210μmol)，抗坏血酸钠(20mg，101μmol)和反式-N，N′-二甲基-1，2-环己烷二胺(60μl，380μmol)并且将混合物在70℃搅拌另外1h min。然后，将反应混合物冷却至室温并且倒到饱和碳酸氢钠水溶液(25mL)上。将其用乙酸乙酯(1x100mL，1x50mL)萃取，将合并的萃取物经硫酸钠干燥并且将硅胶(4g)加入至溶液中。过滤后，将滤液在真空中浓缩，得到绿色固体，为粗产物。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度80∶20至100∶0洗脱)纯化，在真空中干燥(50℃，5毫巴)后，得到标题化合物，为棕色油状物(50mg，27％)。HPLC(方法LCMS_梯度)t_R＝3.4min。MS(ES+)m/z554.5[M+H]。Tert-butyl ((4aS,5R,9S)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-15BB, 210 mg, 340 μmol) was dissolved in 1,4-dioxane (4 mL) and water (1 mL) and sodium azide (225 mg, 3.46 mmol) was added. Then iodinated ketone (I) (35 mg, 184 μmol), sodium ascorbate (17.5 mg, 88.3 μmol) and trans-N, N'-dimethyl-1,2-cyclohexanediamine (45.1 mg, 50 μl, 317 μmol) were added and the dark blue mixture was stirred for 1 h at 70° C. Afterwards, it was cooled to room temperature and a second portion of sodium azide (225 mg, 3.46 mmol), iodinated ketone (I) (40 mg, 210 μmol), sodium ascorbate (20 mg, 101 μmol) and trans-N, N'-dimethyl-1,2-cyclohexanediamine (60 μl, 380 μmol) were added and the mixture was stirred at 70° C. for another 1 h min. At 4 DEG C, 80 DEG C of 40 DEG C of 4-nitropropane-2-ol was added into the 4-nitropropane-2-ol mixture.Afterwards, it is cooled to room temperature and the sodium azide (225mg, 3.46mmol) of the third part is added, iodine ketone (I) (40mg, 210 μmol), sodium ascorbate (20mg, 101 μmol) and trans-N, N '-dimethyl-1,2-cyclohexanediamine (60 μl, 380 μmol) and mixture is stirred into other 1h min at 70 DEG C.Then, reaction mixture is cooled to room temperature and poured onto saturated sodium bicarbonate aqueous solution (25mL). It is extracted with ethyl acetate (1x100mL, 1x50mL), the extract merged is dried over sodium sulfate and silica gel (4g) is added into the solution. After filtration, filtrate is concentrated in a vacuum, obtains green solid, is crude product. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 80:20 to 100:0) to give the title compound as a brown oil (50 mg, 27%) after drying in vacuo (50° C., 5 mbar). HPLC (method LCMS_Gradient) t _R = 3.4 min. MS (ES+) m/z 554.5 [M+H].

Int-31：2-甲基-2-[S-甲基-N-(2-四氢吡喃-2-基氧基乙基)磺酰亚胺基]丙腈的合成Int-31: Synthesis of 2-methyl-2-[S-methyl-N-(2-tetrahydropyran-2-yloxyethyl)sulfonylimino]propionitrile

将氢化钾(矿物油中30％悬浮液，54.7g，410.4mmol)在DME(200.0mL)中的悬浮液冷却至0-5℃(冰浴)。然后将2-甲基-2-(甲基磺酰亚胺基)丙腈(Int-5，30.0g，205.2mmol)在DME(100.0mL)中的溶液逐滴加入至混合物中。这之后，让混合物加温至23℃并且搅拌3h。然后将DME(100.0mL)中的四正丁基溴化铵(3.3g，10.26mmol)和2-(2-溴乙氧基)四氢吡喃(Int-30，85.8g，410.4mmol)加入至反应混合物中。将混合物在23℃搅拌16h。在通过tlc检测起始材料的完全消耗后，将混合物倒入饱和碳酸氢钠水溶液(500mL)中并用乙酸乙酯(300mL)稀释。相分离后，将水相用乙酸乙酯(2x200mL)萃取，将合并的有机萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物，将其通过柱色谱(硅胶，用乙酸乙酯/石油醚50∶50洗脱)纯化，得到标题化合物，为黄色油状物(45.0g，80％产率)。¹H NMR(CDCl₃，400MHz)：δ1.46-1.61(m，4H)，1.67-1.86(m，2H)，1.76(s，6H)，3.09&3.10(2s，3H，非对映异构)，3.31-3.54(m，4H)，3.77-3.91(m，2H)，4.60-4.64(m，1H)。A suspension of potassium hydride (30% suspension in mineral oil, 54.7 g, 410.4 mmol) in DME (200.0 mL) was cooled to 0-5° C. (ice bath). The solution of 2-methyl-2-(methylsulfonylimino)propionitrile (Int-5, 30.0 g, 205.2 mmol) in DME (100.0 mL) was then added dropwise to the mixture. After this, the mixture was allowed to warm to 23° C. and stirred for 3 h. Tetra-n-butylammonium bromide (3.3 g, 10.26 mmol) and 2-(2-bromoethoxy)tetrahydropyran (Int-30, 85.8 g, 410.4 mmol) in DME (100.0 mL) were then added to the reaction mixture. The mixture was stirred at 23° C. for 16 h. After the complete consumption of the starting material was detected by tlc, the mixture was poured into a saturated aqueous sodium bicarbonate solution (500 mL) and diluted with ethyl acetate (300 mL). After phase separation, the aqueous phase was extracted with ethyl acetate (2 x 200 mL), and the combined organic extracts were dried over sodium sulfate, filtered, and concentrated to give the crude product, which was purified by column chromatography (silica gel, eluted with ethyl acetate/petroleum ether 50:50) to afford the title compound as a yellow oil (45.0 g, 80% yield). ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.46-1.61 (m, 4H), 1.67-1.86 (m, 2H), 1.76 (s, 6H), 3.09 & 3.10 (2s, 3H, diastereoisomers), 3.31-3.54 (m, 4H), 3.77-3.91 (m, 2H), 4.60-4.64 (m, 1H).

Int-33(混合物)：2-((2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-(2-羟基乙基)丙基磺酰亚胺基)-2-甲基丙腈的合成Int-33 (Mixture): Synthesis of 2-((2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-(2-hydroxyethyl)propylsulfonylimino)-2-methylpropionitrile

步骤1：(R)-N-((2R)-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-(2-氰基-N-(2-((四氢-2H-吡喃-2-基)氧基)乙基)丙-2-基磺酰亚胺基)丙-2-基)-2-甲基丙-2-亚磺酰胺(Int-32(混合物))Step 1: (R)-N-((2R)-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-(2-cyano-N-(2-((tetrahydro-2H-pyran-2-yl)oxy)ethyl)propan-2-ylsulfonylimino)propan-2-yl)-2-methylpropane-2-sulfinamide (Int-32 (mixture))

在-70℃经10min向2-甲基-2-[S-甲基-N-(2-四氢吡喃-2-基氧基乙基)磺酰亚胺基]丙腈(Int-31，8.0g，29.16mmol)在THF(120.0mL)中的溶液中加入n-BuLi(11.6mL，29.16mmol，2.5N)，将混合物在-70℃搅拌1h。然后经10min将(R，E)-N-(1-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)亚乙基)-2-甲基丙-2-亚磺酰胺(Int-9，12.7g，29.16mmol)在THF(30.0mL)中的溶液加入至反应混合物中。然后将混合物在-70℃搅拌4h。通过加入饱和氯化铵水溶液(100mL)将反应混合物猝灭并且用乙酸乙酯(2x100mL)萃取。将有机层用盐水(100mL)洗涤，经硫酸钠干燥。过滤后，浓缩滤液，得到粗产物。将粗产物通过柱色谱(硅胶，用乙酸乙酯/石油醚50∶50洗脱)纯化，得到标题化合物(5.0g，24％产率)，为黄色油状物，并且为非对映异构体的混合物。To a solution of 2-methyl-2-[S-methyl-N-(2-tetrahydropyran-2-yloxyethyl)sulfonylimino]propionitrile (Int-31, 8.0 g, 29.16 mmol) in THF (120.0 mL) was added n-BuLi (11.6 mL, 29.16 mmol, 2.5 N) at -70°C over 10 min, and the mixture was stirred at -70°C for 1 h. A solution of (R,E)-N-(1-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)ethylidene)-2-methylpropane-2-sulfenamide (Int-9, 12.7 g, 29.16 mmol) in THF (30.0 mL) was then added to the reaction mixture over 10 min. The mixture was then stirred at -70°C for 4 h. By adding saturated aqueous ammonium chloride solution (100mL), the reaction mixture is quenched and extracted with ethyl acetate (2x100mL).The organic layer is washed with brine (100mL), dried over sodium sulfate.After filtration, the filtrate is concentrated to give a crude product.The crude product is purified by column chromatography (silica gel, eluted with ethyl acetate/petroleum ether 50: 50) to give the title compound (5.0g, 24% yield), which is a yellow oil and a mixture of diastereomers.

步骤2：2-((2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-(2-羟基乙基)丙基磺酰亚胺基)-2-甲基丙腈(Int-33(混合物))Step 2: 2-((2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-(2-hydroxyethyl)propylsulfonylimino)-2-methylpropionitrile (Int-33 (mixture))

在0℃向(R)-N-((2R)-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-(2-氰基-N-(2-((四氢-2H-吡喃-2-基)氧基)乙基)丙-2-基磺酰亚胺基)丙-2-基)-2-甲基丙-2-亚磺酰胺(Int-32(混合物)，25.0g，35.2mmol)在甲醇(200mL)中的溶液中加入氯化氢的甲醇溶液(4N，40.0mL)。将反应混合物在15℃搅拌1h。之后，通过加入碳酸钠水溶液(2M)将反应混合物碱化，直到pH 8-9，并且用乙酸乙酯(2x200mL)萃取。将合并的有机层经硫酸钠干燥，过滤并在真空中浓缩。将粗产物通过柱色谱(硅胶，用乙酸乙酯洗脱)纯化，得到标题化合物(16.3g，75％产率)，为黄色固体，为非对映异构体的混合物。To a solution of (R)-N-((2R)-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-(2-cyano-N-(2-((tetrahydro-2H-pyran-2-yl)oxy)ethyl)propan-2-ylsulfonylimino)propan-2-yl)-2-methylpropane-2-sulfinamide (Int-32 (mixture), 25.0 g, 35.2 mmol) in methanol (200 mL) was added a methanolic solution of hydrogen chloride (4N, 40.0 mL). The reaction mixture was stirred at 15 ° C for 1 h. Afterwards, the reaction mixture was basified by the addition of aqueous sodium carbonate (2 M) until pH 8-9 and extracted with ethyl acetate (2x200 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, eluting with ethyl acetate) to give the title compound (16.3 g, 75% yield) as a yellow solid as a mixture of diastereomers.

Int-35A&Int-35B：((1R，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((2-羟基乙基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-35A)和((1S，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((2-羟基乙基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-35B)的合成Int-35A & Int-35B: Synthesis of tert-butyl ((1R,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((2-hydroxyethyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-35A) and tert-butyl ((1S,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((2-hydroxyethyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-35B)

步骤1：(3R)-5-氨基-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((2-羟基乙基)亚氨基)-3，6，6-三甲基-3，6-二氢-2H-1，4-噻嗪1-氧化物(Int-34(混合物))Step 1: (3R)-5-amino-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((2-hydroxyethyl)imino)-3,6,6-trimethyl-3,6-dihydro-2H-1,4-thiazine 1-oxide (Int-34 (mixture))

向2-((2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-(2-羟基乙基)丙基磺酰亚胺基)-2-甲基丙腈(Int-33(混合物)，15.0g，28.7mmol)在乙醇(200mL)中的悬浮液中加入氯化铜(I)(3.13g，31.6mmol)。然后，将混合物在70℃搅拌2h。将反应混合物倒入盐水(100mL)和氨水(100mL)的混合物中。将得到的混合物用乙酸乙酯(2x100mL)萃取，将合并的有机层经Na₂SO₄干燥，过滤并在真空中浓缩，得到粗产物，为黄色胶质，在不进一步纯化的情况下将其用于下一步骤(15g)。To a suspension of 2-((2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-(2-hydroxyethyl)propylsulfonylimino)-2-methylpropionitrile (Int-33 (mixture), 15.0 g, 28.7 mmol) in ethanol (200 mL) was added copper (I) chloride (3.13 g, 31.6 mmol). The mixture was then stirred at 70° C. for 2 h. The reaction mixture was poured into a mixture of brine (100 mL) and aqueous ammonia (100 mL). The resulting mixture was extracted with ethyl acetate (2×100 mL), and the combined organic layers were dried over Na ₂ SO ₄ , filtered, and concentrated in vacuo to give the crude product as a yellow gum, which was used in the next step without further purification (15 g).

步骤2：((1R，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((2-羟基乙基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-35A)和((1S，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((2-羟基乙基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-35B)Step 2: tert-Butyl ((1R,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((2-hydroxyethyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-35A) and tert-butyl ((1S,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((2-hydroxyethyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-35B)

向(3R)-5-氨基-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((2-羟基乙基)亚氨基)-3，6，6-三甲基-3，6-二氢-2H-1，4-噻嗪1-氧化物(Int-34(混合物)，来自前述步骤的粗产物，30.0g，57.2mmol)在四氢呋喃(400mL)和水(80mL)中的悬浮液中加入固体碳酸氢钠(6.3g，74.7mmol)，接着加入Boc-酐(13.8g，63.3mmol)和4-(二甲基氨基)-吡啶(351mg，2.8mmol)。将混合物在15℃搅拌18h。将反应混合物用水(200mL)稀释并用乙酸乙酯(2x200mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并在真空中浓缩。将粗产物通过柱色谱(硅胶，用乙酸乙酯/石油醚25_∶75洗脱)纯化，得到标题化合物，为分离得非对映异构体。首先洗脱的非对映异构体(Int-35A)分离为黄色固体(9.0g，25％)，并且获得其次洗脱的非对映异构体(Int-35B)为黄色胶质(11.0g，31％)。经2个步骤，合并的产率是56％。To a suspension of (3R)-5-amino-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((2-hydroxyethyl)imino)-3,6,6-trimethyl-3,6-dihydro-2H-1,4-thiazine 1-oxide (Int-34 (mixture), crude product from the previous step, 30.0 g, 57.2 mmol) in tetrahydrofuran (400 mL) and water (80 mL) was added solid sodium bicarbonate (6.3 g, 74.7 mmol), followed by Boc-anhydride (13.8 g, 63.3 mmol) and 4-(dimethylamino)-pyridine (351 mg, 2.8 mmol). The mixture was stirred at 15 ° C for 18 h. The reaction mixture was diluted with water (200 mL) and extracted with ethyl acetate (2×200 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, eluting with ethyl acetate/petroleum ether _25:75 ) to afford the title compound as separated diastereomers. The first-eluting diastereomer (Int-35A) was isolated as a yellow solid (9.0 g, 25%), and the second-eluting diastereomer (Int-35B) was obtained as a yellow gum (11.0 g, 31%). The combined yield over two steps was 56%.

Int-35A：¹H NMR(CDCl₃，400MHz)：δ0.82-0.90(m，6H)，0.93-1.00(m，9H)，1.55(s，9H)，1.64(s，3H)，1.79(s，3H)，1.83(s，3H)，2.12(t，J＝6.3Hz，1H)，2.67(ddd，J＝3.5，6.0，12.5Hz，1H)，3.09(ddd，J＝3.5，6.8，12.5Hz，1H)，3.24-3.34(m，1H)，3.37-3.46(m，1H)，3.60(d，J＝15.1Hz，1H)，4.49(d，J＝15.1Hz，1H)，7.36(d，J＝2.5Hz，1H)，11.11(s，1H)。Int-35A: ¹ H NMR (CDCl ₃ , 400MHz): δ0.82-0.90 (m, 6H), 0.93-1.00 (m, 9H), 1.55 (s, 9H), 1.64 (s, 3H), 1.7 9 (s, 3H), 1.83 (s, 3H), 2.12 (t, J=6.3Hz, 1H), 2.67 (ddd, J=3.5, 6.0, 12.5Hz, 1H) , 3.09 (ddd, J=3.5, 6.8, 12.5Hz, 1H), 3.24-3.34 (m, 1H), 3.37-3.46 (m, 1H), 3.60 (d, J=15.1Hz, 1H), 4.49 (d, J=15.1Hz, 1H), 7.36 (d, J=2.5Hz, 1H), 11.11 (s, 1H).

Int-35B：¹H NMR(CDCl₃，400MHz)：δ0.80-0.90(m，6H)，0.91-1.00(m，9H)，1.53(s，9H)，1.66(s，3H)，1.73(s，3H)，1.85(s，3H)，2.39-2.47(m，1H)，3.29-3.33(m，2H)，3.58(d，J＝15.3Hz，1H)，3.62-3.68(m，2H)，4.31(d，J＝15.1Hz，1H)，7.36(d，J＝2.5Hz，1H)，11.16(s，1H)。Int-35B: ¹ H NMR (CDCl ₃ , 400MHz): δ0.80-0.90 (m, 6H), 0.91-1.00 (m, 9H), 1.53 (s, 9H), 1.66 (s, 3H), 1.73 (s, 3H), 1.85 (s, 3H), 2.39-2.47 (m, 1H), 3.29-3.33 (m, 2H), 3.58 (d, J=15.3Hz, 1H), 3.62-3.68 (m, 2H), 4.31 (d, J=15.1Hz, 1H), 7.36 (d, J=2.5Hz, 1H), 11.16 (s, 1H).

Int-37AB：((3aS，4R，8R)-4-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯的合成Int-37AB: Synthesis of tert-butyl ((3aS,4R,8R)-4-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate

步骤1：4-甲基苯磺酸2-(((1R，3R)-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5-((叔丁氧基羰基)氨基)-3，6，6-三甲基-1-氧代-3，6-二氢-2H-1，4-噻嗪-1-亚基)氨基)乙酯(Int-36A)Step 1: 2-(((1R,3R)-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5-((tert-butoxycarbonyl)amino)-3,6,6-trimethyl-1-oxo-3,6-dihydro-2H-1,4-thiazin-1-ylidene)amino)ethyl 4-methylbenzenesulfonate (Int-36A)

在0℃向((1R，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((2-羟基乙基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-35A，9.0g，14.5mmol)在二氯甲烷(150mL)中的溶液中加入甲苯磺酰氯(3.3g，17.4mmol)，三乙胺(2.9g，29.0mmol)和4-(二甲基氨基)-吡啶(88.5mg，0.73mmol)。将得到的混合物在15℃搅拌16h。之后，将混合物倒入水(200mL)中并用乙酸乙酯(2x 100mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用乙酸乙酯/石油醚25∶75洗脱)纯化，得到标题化合物，为黄色固体(10.5g，95％)。¹H NMR(CDCl₃，400MHz)：δ0.81-0.90(m，6H)，0.92-1.00(m，9H)，1.54(s，9H)，1.59(s，3H)，1.82(s，3H)，2.44(s，3H)，2.86-2.94(m，1H)，3.16-3.24(m，1H)，3.50(d，J＝15.1Hz，1H)，3.70-3.78(m，1H)，3.82-3.89(m，1H)，4.46(d，J＝15.1Hz，1H)，7.33(d，J＝8.0Hz，2H)，7.39(d，J＝2.5Hz，1H)，7.74(d，J＝8.3Hz，2H)，11.14(s，1H)。To a solution of tert-butyl ((1R,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((2-hydroxyethyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-35A, 9.0 g, 14.5 mmol) in dichloromethane (150 mL) was added tosyl chloride (3.3 g, 17.4 mmol), triethylamine (2.9 g, 29.0 mmol) and 4-(dimethylamino)-pyridine (88.5 mg, 0.73 mmol) at 0°C. The resulting mixture was stirred at 15°C for 16 h. Afterwards, the mixture was poured into water (200 mL) and extracted with ethyl acetate (2 x 100 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by column chromatography (silica gel, eluted with ethyl acetate/petroleum ether 25:75) to give the title compound as a yellow solid (10.5 g, 95%). ¹ H NMR (CDCl ₃ , 400 MHz): δ 0.81-0.90 (m, 6H), 0.92-1.00 (m, 9H), 1.54 (s, 9H), 1.59 (s, 3H), 1.82 (s, 3H), 2.44 (s, 3H), 2.86-2.94 (m, 1H), 3.16-3.24 (m, 1H), 3.50 (d, J=15 .1Hz, 1H), 3.70-3.78 (m, 1H), 3.82-3.89 (m, 1H), 4.46 (d, J=15.1Hz, 1H), 7.33 (d, J=8.0Hz, 2H), 7.39 (d, J=2.5Hz, 1H), 7.74 (d, J=8.3Hz, 2H), 11.14 (s, 1H).

步骤2：((3aS，4R，8R)-4-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-37AB)Step 2: tert-Butyl ((3aS,4R,8R)-4-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-37AB)

在-70℃经5min向4-甲基苯磺酸2-(((1R，3R)-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5-((叔丁氧基羰基)氨基)-3，6，6-三甲基-1-氧代-3，6-二氢-2H-1，4-噻嗪-1-亚基)氨基)乙酯(Int-36A，8.5g，10.9mmol)在无水THF(150mL)中的溶液中逐滴加入六甲基二硅基氨基锂(LHMDS)在THF中的溶液(1M，32.7mL，32.7mmol)。然后，将黄色溶液加温至0℃(冰浴)并搅拌2h。之后，将反应混合物倒入饱和氯化铵水溶液(150mL)中并用乙酸乙酯(2x150mL)萃取。将合并的萃取物用盐水(200mL)洗涤，经硫酸钠干燥并浓缩，得到粗产物(6.48g)，为单个非对映异构体，在不进一步纯化的情况下将其用于下一步骤。¹H NMR(CDCl₃，400MHz)：δ0.84-0.91(m，6H)，0.93-0.99(m，9H)，1.57(s，9H)，1.70-1.80(m，1H)，1.83(s，3H)，1.91-2.01(m，1H)，1.95(s，3H)，2.11(s，3H)，3.54(dd，J＝7.5，10.5Hz，1H)，3.69(ddd，J＝5.0，10.5，10.5Hz，1H)，4.24(ddd，J＝2.5，7.3，12.0Hz，1H)，7.47(d，J＝2.5Hz，1H)，12.09(s，1H)。To a solution of 2-(((1R,3R)-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5-((tert-butoxycarbonyl)amino)-3,6,6-trimethyl-1-oxo-3,6-dihydro-2H-1,4-thiazin-1-ylidene)amino)ethyl 4-methylbenzenesulfonate (Int-36A, 8.5 g, 10.9 mmol) in anhydrous THF (150 mL) was added a solution of lithium hexamethyldisilazide (LHMDS) in THF (1 M, 32.7 mL, 32.7 mmol) dropwise at -70°C over 5 min. The yellow solution was then warmed to 0°C (ice bath) and stirred for 2 h. The reaction mixture was then poured into saturated aqueous ammonium chloride (150 mL) and extracted with ethyl acetate (2 x 150 mL). The combined extracts were washed with brine (200 mL), dried over sodium sulfate and concentrated to give the crude product (6.48 g) as a single diastereomer, which was used in the next step without further purification. ¹ H NMR (CDCl ₃ , 400MHz): δ0.84-0.91 (m, 6H), 0.93-0.99 (m, 9H), 1.57 (s, 9H), 1.70-1.80 (m, 1H), 1.83 (s, 3H), 1.91-2.01 (m, 1H), 1.95 (s, 3H), 2.11 (s, 3H) , 3.54 (dd, J=7.5, 10.5Hz, 1H), 3.69 (ddd, J=5.0, 10.5, 10.5Hz, 1H), 4.24 (ddd, J=2.5, 7.3, 12.0Hz, 1H), 7.47 (d, J=2.5Hz, 1H), 12.09 (s, 1H).

Int-37BA：((3aR，4R，8S)-4-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯的合成Int-37BA: Synthesis of tert-butyl ((3aR,4R,8S)-4-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate

步骤1：4-甲基苯磺酸2-(((1S，3R)-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5-((叔丁氧基羰基)氨基)-3，6，6-三甲基-1-氧代-3，6-二氢-2H-1，4-噻嗪-1-亚基)氨基)乙酯(Int-36B)Step 1: 2-(((1S,3R)-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5-((tert-butoxycarbonyl)amino)-3,6,6-trimethyl-1-oxo-3,6-dihydro-2H-1,4-thiazin-1-ylidene)amino)ethyl 4-methylbenzenesulfonate (Int-36B)

在0℃向((1S，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((2-羟基乙基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-35B，11.0g，17.7mmol)在二氯甲烷(150mL)中的溶液中加入甲苯磺酰氯(4.0g，21.2mmol)，三乙胺(3.6g，35.4mmol)和4-(二甲基氨基)-吡啶(107.9mg，0.89mmol)。将得到的混合物在15℃搅拌16h。之后，将混合物倒入水(200mL)中并用乙酸乙酯(2x100mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用乙酸乙酯/石油醚25∶75洗脱)纯化，得到标题化合物，为黄色固体(13.0g，95％)。¹H NMR(CDCl₃，400MHz)：δ0.81-0.91(m，6H)，0.92-1.00(m，9H)，1.54(s，9H)，1.59(s，3H)，1.64(s，3H)，1.82(s，3H)，2.45(s，3H)，3.30-3.43(m，2H)，3.47(d，J＝15.1Hz，1H)，4.03-4.13(m，2H)，4.24(d，J＝15.1Hz，1H)，7.34-7.38(m，3H)，7.80(d，J＝8.3Hz，2H)，11.15(s，1H)。To a solution of tert-butyl ((1S,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((2-hydroxyethyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-35B, 11.0 g, 17.7 mmol) in dichloromethane (150 mL) was added tosyl chloride (4.0 g, 21.2 mmol), triethylamine (3.6 g, 35.4 mmol) and 4-(dimethylamino)-pyridine (107.9 mg, 0.89 mmol) at 0°C. The resulting mixture was stirred at 15°C for 16 h. Afterwards, the mixture was poured into water (200 mL) and extracted with ethyl acetate (2x100 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by column chromatography (silica gel, eluted with ethyl acetate/petroleum ether 25:75) to give the title compound as a yellow solid (13.0 g, 95%). ¹ H NMR (CDCl ₃ , 400MHz): δ0.81-0.91 (m, 6H), 0.92-1.00 (m, 9H), 1.54 (s, 9H), 1.59 (s, 3H), 1.64 (s, 3H), 1.82 (s, 3H), 2.45 (s, 3H), 3.30-3.43 (m , 2H), 3.47 (d, J=15.1Hz, 1H), 4.03-4.13 (m, 2H), 4.24 (d, J=15.1Hz, 1H), 7.34-7.38 (m, 3H), 7.80 (d, J=8.3Hz, 2H), 11.15 (s, 1H).

步骤2：((3aR，4R，8S)-4-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-37BA)Step 2: tert-Butyl ((3aR,4R,8S)-4-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-37BA)

在-70℃经5min向4-甲基苯磺酸2-(((1S，3R)-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5-((叔丁氧基羰基)氨基)-3，6，6-三甲基-1-氧代-3，6-二氢-2H-1，4-噻嗪-1-亚基)氨基)乙酯(Int-36B，11.0g，14.2mmol)在无水THF(150mL)中的溶液中逐滴加入六甲基二硅基氨基锂(LHMDS)在THF(1M，42.6mL，42.6mmol)中的溶液。然后，将黄色溶液加温至0℃(冰浴)并且搅拌2h。之后，将反应混合物倒入饱和氯化铵水溶液(150mL)中并用乙酸乙酯(2x150mL)萃取。将合并的萃取物用盐水(200mL)洗涤，经硫酸钠干燥并浓缩，得到粗产物(6.77g)，为单个非对映异构体，在不进一步纯化的情况下将其用于下一步骤。To a solution of 2-(((1S,3R)-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5-((tert-butoxycarbonyl)amino)-3,6,6-trimethyl-1-oxo-3,6-dihydro-2H-1,4-thiazin-1-ylidene)amino)ethyl 4-methylbenzenesulfonate (Int-36B, 11.0 g, 14.2 mmol) in anhydrous THF (150 mL) was added dropwise a solution of lithium hexamethyldisilazide (LHMDS) in THF (1 M, 42.6 mL, 42.6 mmol) at -70°C over 5 min. The yellow solution was then warmed to 0°C (ice bath) and stirred for 2 h. The reaction mixture was then poured into saturated aqueous ammonium chloride solution (150 mL) and extracted with ethyl acetate (2 x 150 mL). The combined extracts were washed with brine (200 mL), dried over sodium sulfate and concentrated to give the crude product (6.77 g) as a single diastereomer, which was used in the next step without further purification.

Int-39AB：((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯的合成Int-39AB: Synthesis of tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate

步骤1：((3aS，4R，8R)-4-(6-溴-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-38AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(6-bromo-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-38AB)

在16℃向((3aS，4R，8R)-4-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-37AB，6.0g，来自前一步骤的粗产物，约9.94mmol)在THF(60mL)和DMF(10mL)中的悬浮液中加入氟化钾(1.15g，19.8mmol)和乙酸(1.18g，19.8mmol)，将混合物在30℃搅拌3h。将反应混合物用碳酸氢钠水溶液(100mL)稀释，用乙酸乙酯(2x100mL)萃取。将有机层用盐水(100mL)洗涤并经硫酸钠干燥。过滤后，将滤液在真空中浓缩，得到粗产物。将粗产物悬浮在乙酸乙酯(15mL)和石油醚(60mL)的混合物中并在15℃搅拌30min。将沉淀过滤出，用乙酸乙酯/石油醚(1∶5v/v，30mL)的混合物洗涤并在真空中干燥，得到标题化合物，为黄色固体(4.87g，经2个步骤85％)。¹H NMR(CDCl₃，400MHz)：δ1.59(s，9H)，1.61-1.65(m，1H)，1.85(s，3H)，1.96(s，3H)，2.01-2.09(m，1H)，2.14(s，3H)，3.56(dd，J＝7.7，10.6Hz，1H)，3.70(dd，J＝4.9，10.6Hz，1H)，4.24(ddd，J＝2.5，7.1，12.1Hz，1H)，7.42(dd，J＝8.5，10.5Hz，1H)，7.57(dd，J＝3.3，8.5Hz，1H)，12.13(s，1H)。To a suspension of tert-butyl ((3aS,4R,8R)-4-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-37AB, 6.0 g, crude product from the previous step, approximately 9.94 mmol) in THF (60 mL) and DMF (10 mL) was added potassium fluoride (1.15 g, 19.8 mmol) and acetic acid (1.18 g, 19.8 mmol) at 16° C., and the mixture was stirred at 30° C. for 3 h. The reaction mixture was diluted with aqueous sodium bicarbonate (100 mL) and extracted with ethyl acetate (2×100 mL). The organic layer was washed with brine (100 mL) and dried over sodium sulfate. After filtration, the filtrate was concentrated in vacuo to give a crude product. The crude product was suspended in a mixture of ethyl acetate (15 mL) and petroleum ether (60 mL) and stirred at 15 ° C for 30 min. The precipitate was filtered out, washed with a mixture of ethyl acetate/petroleum ether (1: 5 v/v, 30 mL) and dried in vacuo to give the title compound as a yellow solid (4.87 g, 85% over 2 steps). ¹ H NMR (CDCl ₃ , 400MHz): δ1.59 (s, 9H), 1.61-1.65 (m, 1H), 1.85 (s, 3H), 1.96 (s, 3H), 2.01-2.09 (m, 1H), 2.14 (s, 3H), 3.56 (dd, J=7.7, 10.6Hz, 1H), 3. 70 (dd, J=4.9, 10.6Hz, 1H), 4.24 (ddd, J=2.5, 7.1, 12.1Hz, 1H), 7.42 (dd, J=8.5, 10.5Hz, 1H), 7.57 (dd, J=3.3, 8.5Hz, 1H), 12.13 (s, 1H).

步骤2：((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB)Step 2: tert-Butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB)

向((3aS，4R，8R)-4-(6-溴-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-38AB，3.0g，6.13mmol)在二烷(30.0mL)和水(10.0mL)中的混合物中加入碘化铜(I)(1.27g，6.7mmol)，抗坏血酸钠(0.73g，3.7mmol)，叠氮化钠(6.0g，92.0mmol)和反式-N，N′-二甲基-1，2-环己烷二胺(1.57g，11.03mmol)。然后将深蓝色混合物在70℃搅拌1h。将反应混合物用碳酸氢钠水溶液(100mL)稀释并用乙酸乙酯(2x100mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用乙酸乙酯/石油醚2∶1洗脱)纯化，在真空中干燥后得到标题化合物，为黄色固体(2.3g，89％产率)。¹H NMR(CDCl₃，400MHz)：δ1.53-1.63(m，1H)，1.57(s，9H)，1.84(s，3H)，1.95(s，3H)，2.06-2.15(m，1H)，2.09(s，3H)，3.53(dd，J＝7.6，10.6Hz，1H)，3.68(ddd，J＝4.8，10.5，10.5Hz，1H)，4.25(ddd，J＝1.9，7.0，12.2Hz，1H)，4.60(s，2H)，6.50(dd，J＝2.5，8.8Hz，1H)，7.28(dd，J＝8.9，10.5Hz，1H)，12.29(s，1H)。To a mixture of tert-butyl ((3aS,4R,8R)-4-(6-bromo-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-38AB, 3.0 g, 6.13 mmol) in dioxane (30.0 mL) and water (10.0 mL) were added copper (I) iodide (1.27 g, 6.7 mmol), sodium ascorbate (0.73 g, 3.7 mmol), sodium azide (6.0 g, 92.0 mmol) and trans-N,N′-dimethyl-1,2-cyclohexanediamine (1.57 g, 11.03 mmol). The dark blue mixture was then stirred at 70° C. for 1 h. The reaction mixture was diluted with aqueous sodium bicarbonate (100 mL) and extracted with ethyl acetate (2x100 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated to give a crude product. The crude product was purified by column chromatography (silica gel, eluted with ethyl acetate/petroleum ether 2: 1) to give the title compound as a yellow solid (2.3 g, 89% yield) after drying in vacuo. ¹ H NMR (CDCl ₃ , 400MHz): δ1.53-1.63 (m, 1H), 1.57 (s, 9H), 1.84 (s, 3H), 1.95 (s, 3H), 2. 06-2.15 (m, 1H), 2.09 (s, 3H), 3.53 (dd, J＝7.6, 10.6Hz, 1H), 3.68 (ddd, J＝ 4.8, 10.5, 10.5Hz, 1H), 4.25 (ddd, J=1.9, 7.0, 12.2Hz, 1H), 4.60 (s, 2H), 6.50 (dd, J=2.5, 8.8Hz, 1H), 7.28 (dd, J=8.9, 10.5Hz, 1H), 12.29 (s, 1H).

Int-39BA：((3aR，4R，8S)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯的合成Int-39BA: Synthesis of tert-butyl ((3aR,4R,8S)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate

步骤1：((3aR，4R，8S)-4-(6-溴-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-38BA)Step 1: tert-Butyl ((3aR,4R,8S)-4-(6-bromo-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-38BA)

在16℃向((3aR，4R，8S)-4-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-37BA，6.0g，来自前一步骤的粗产物，约9.94mmol)在THF(60mL)和DMF(10mL)中的悬浮液中加入氟化钾(1.15g，19.8mmol)和乙酸(1.18g，19.8mmol)，将混合物在30℃搅拌3h。将反应混合物用碳酸氢钠水溶液(100mL)稀释，用乙酸乙酯(2x100mL)萃取。将有机层用盐水(100mL)洗涤并经硫酸钠干燥。过滤后，将滤液在真空中浓缩，得到粗产物。将粗产物悬浮在乙酸乙酯(15mL)和石油醚(60mL)的混合物中并在15℃搅拌30min。将沉淀过滤出，用乙酸乙酯/石油醚(1∶5v/v，30mL)的混合物洗涤并在真空中干燥，得到标题化合物，为黄色固体(4.12g，经2个步骤68％)。¹H NMR(CDCl₃，400MHz)：δ0.91(s，3H)，1.55(s，9H)，1.72(s.3H)，1.75(s，3H)，2.07-2.19(m，1H)，2.54-2.62(m，1H)，3.71-3.85(m，2H)，5.07(dd，J＝7.2，10.9Hz，1H)，7.36(dd，J＝8.5，10.3Hz，1H)，7.53(dd，J＝3.0，8.5Hz，1H)，10.99(s，1H)。To a suspension of tert-butyl ((3aR,4R,8S)-4-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-37BA, 6.0 g, crude product from the previous step, approximately 9.94 mmol) in THF (60 mL) and DMF (10 mL) was added potassium fluoride (1.15 g, 19.8 mmol) and acetic acid (1.18 g, 19.8 mmol) at 16° C., and the mixture was stirred at 30° C. for 3 h. The reaction mixture was diluted with aqueous sodium bicarbonate (100 mL) and extracted with ethyl acetate (2×100 mL). The organic layer was washed with brine (100 mL) and dried over sodium sulfate. After filtration, the filtrate was concentrated in vacuo to give a crude product. The crude product was suspended in a mixture of ethyl acetate (15 mL) and petroleum ether (60 mL) and stirred at 15 ° C for 30 min. The precipitate was filtered out, washed with a mixture of ethyl acetate/petroleum ether (1: 5 v/v, 30 mL) and dried in vacuo to give the title compound as a yellow solid (4.12 g, 68% over 2 steps). ¹ H NMR (CDCl ₃ , 400MHz): δ0.91 (s, 3H), 1.55 (s, 9H), 1.72 (s.3H), 1.75 (s, 3H), 2.07-2.19 (m, 1H), 2.54-2.62 (m, 1H), 3.71-3.8 5 (m, 2H), 5.07 (dd, J=7.2, 10.9Hz, 1H), 7.36 (dd, J=8.5, 10.3Hz, 1H), 7.53 (dd, J=3.0, 8.5Hz, 1H), 10.99 (s, 1H).

步骤2：((3aR，4R，8S)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39BA)Step 2: tert-Butyl ((3aR,4R,8S)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39BA)

向((3aR，4R，8S)-4-(6-溴-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-38BA，5.5g，11.2mmol)在二烷(100.0mL)和水(30.0mL)中的混合物中加入碘化铜(I)(2.3g，12.3mmol)，抗坏血酸钠(1.3g，6.7mmol)，叠氮化钠(11.0g，168.0mmol)和反式-N，N′-二甲基-1，2-环己烷二胺(2.88g，20.23mmol)。然后在70℃将深蓝色混合物搅拌1h。将反应混合物用碳酸氢钠水溶液(200mL)稀释并用乙酸乙酯(2x150mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用乙酸乙酯/石油醚2∶1洗脱)纯化，在真空中干燥后得到标题化合物，为黄色固体(4.5g，94％产率)。¹H NMR(CDCl₃，400MHz)：δ0.93(s，3H)，1.52(s，9H)，1.66(s，3H)，1.73(s，3H)，2.03-2.15(m，1H)，2.47-2.57(m，1H)，3.69-3.79(m，2H)，4.54(s，2H)，5.16(dd，J＝7.2，10.9Hz，1H)，6.46(dd，J＝2.3，8.8Hz，1H)，7.20(dd，J＝8.8，10.8Hz，1H)，10.90(s，1H)。To a mixture of tert-butyl ((3aR,4R,8S)-4-(6-bromo-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-38BA, 5.5 g, 11.2 mmol) in dioxane (100.0 mL) and water (30.0 mL) were added copper (I) iodide (2.3 g, 12.3 mmol), sodium ascorbate (1.3 g, 6.7 mmol), sodium azide (11.0 g, 168.0 mmol) and trans-N,N′-dimethyl-1,2-cyclohexanediamine (2.88 g, 20.23 mmol). The dark blue mixture was then stirred at 70° C. for 1 h. The reaction mixture was diluted with aqueous sodium bicarbonate (200 mL) and extracted with ethyl acetate (2x150 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated to give a crude product. The crude product was purified by column chromatography (silica gel, eluted with ethyl acetate/petroleum ether 2: 1) to give the title compound as a yellow solid (4.5 g, 94% yield) after drying in vacuo. ¹ H NMR (CDCl ₃ , 400MHz): δ0.93 (s, 3H), 1.52 (s, 9H), 1.66 (s, 3H), 1.73 (s, 3H), 2.03-2.15 (m, 1H), 2.47-2.57 (m, 1H), 3.69-3.79 (m, 2 H), 4.54 (s, 2H), 5.16 (dd, J=7.2, 10.9Hz, 1H), 6.46 (dd, J=2.3, 8.8Hz, 1H), 7.20 (dd, J=8.8, 10.8Hz, 1H), 10.90 (s, 1H).

Int-43：2-(N-(2，2-二氟-3-((四氢-2H-吡喃-2-基)氧基)丙基)-S-甲基磺酰亚胺基)-2-甲基丙腈的合成Int-43: Synthesis of 2-(N-(2,2-difluoro-3-((tetrahydro-2H-pyran-2-yl)oxy)propyl)-S-methylsulfonylimino)-2-methylpropionitrile

步骤1：2，2-二氟-3-((四氢-2H-吡喃-2-基)氧基)丙-1-醇(Int-41)Step 1: 2,2-Difluoro-3-((tetrahydro-2H-pyran-2-yl)oxy)propan-1-ol (Int-41)

将2，2-二氟丙-1，3-二醇(1.00g，8.92mmol)悬浮在环己烷(50mL)中，加入2，3-二氢吡喃(2.48g，2.7ml，29.5mmol)，接着加入硫酸氢钠水溶液(5mol/L，1.8mL，9mmol)并且将两相混合物在30℃搅拌90min。之后，加入固体碳酸钠(8g，75.5mmol)，将反应混合物通过加入叔丁基甲醚(20mL)稀释并在室温搅拌30min。将其过滤并将滤液在真空中浓缩(55℃/5毫巴)。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度20∶80至40∶60洗脱)纯化，在真空中干燥(50℃，5毫巴)后得到标题化合物，为无色油状物(1.2g，69％)。¹H NMR(CDCl₃，400MHz)：δ1.49-1.65(m，4H)，1.72-1.88(m，2H)，2.58(dd，J＝7.1，7.7Hz，1H)，3.51-3.59(m，1H)，3.74-4.07(m，5H)，4.62-4.66(m，1H)。2,2-Difluoropropane-1,3-diol (1.00 g, 8.92 mmol) was suspended in cyclohexane (50 mL), 2,3-dihydropyran (2.48 g, 2.7 ml, 29.5 mmol) was added, followed by aqueous sodium bisulfate solution (5 mol/L, 1.8 mL, 9 mmol) and the two-phase mixture was stirred at 30° C. for 90 min. Afterwards, solid sodium carbonate (8 g, 75.5 mmol) was added, the reaction mixture was diluted by adding tert-butyl methyl ether (20 mL) and stirred at room temperature for 30 min. It was filtered and the filtrate was concentrated in vacuo (55° C./5 mbar). The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 20:80 to 40:60) to give the title compound as a colorless oil (1.2 g, 69%) after drying in vacuo (50° C., 5 mbar). ¹ H NMR (CDCl ₃ , 400MHz): δ1.49-1.65 (m, 4H), 1.72-1.88 (m, 2H), 2.58 (dd, J=7.1, 7.7Hz, 1H), 3.51-3.59 (m, 1H), 3.74-4.07 (m, 5H), 4.62-4.66 (m, 1H).

步骤2：三氟甲磺酸2，2-二氟-3-羟基丙酯(Int-42)Step 2: 2,2-Difluoro-3-hydroxypropyl trifluoromethanesulfonate (Int-42)

将2，2-二氟-3-((四氢-2H-吡喃-2-基)氧基)丙-1-醇(Int-41，1.20g，6.12mmol)溶解在二氯甲烷(15mL)中并将溶液冷却至0-5℃(冰浴)。经15min加入吡啶(685mg，700μl，8.65mmol)，接着加入三氟甲磺酸酐(2g，1.2ml，6.96mmol)在二氯甲烷(2mL)中的溶液。将反应混合物搅拌45min。之后，将其倒入碳酸氢钠饱和水溶液(50mL)中并用叔丁基甲醚(1x100mL，1x50mL)萃取。将合并的有机萃取物用盐水(50mL)洗涤，干燥(硫酸钠)并在真空中浓缩，得到棕色油状物，为粗产物。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度2∶98至10∶90洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为粘性无色油状物(1.54g，77％)。¹H NMR(CDCl₃，400MHz)：δ1.48-1.68(m，4H)，1.69-1，86(m，2H)，3.52-3.60(m，1H)，3.68-3.84(m，2H)，4.02(ddd，J＝10.7，11.5，14.5Hz，1H)，4.63-4.76(m，3H)。2,2-difluoro-3-((tetrahydro-2H-pyran-2-yl)oxy)propan-1-ol (Int-41, 1.20 g, 6.12 mmol) was dissolved in dichloromethane (15 mL) and the solution was cooled to 0-5 ° C (ice bath). Pyridine (685 mg, 700 μl, 8.65 mmol) was added over 15 min, followed by a solution of trifluoromethanesulfonic anhydride (2 g, 1.2 ml, 6.96 mmol) in dichloromethane (2 mL). The reaction mixture was stirred for 45 min. Afterwards, it was poured into a saturated aqueous solution of sodium bicarbonate (50 mL) and extracted with tert-butyl methyl ether (1x100 mL, 1x50 mL). The combined organic extracts were washed with brine (50 mL), dried (sodium sulfate) and concentrated in vacuo to give a brown oil as a crude product. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 2:98 to 10:90) to afford the title compound as a viscous colorless oil (1.54 g, 77%) after drying in vacuo (40° C., 5 mbar). ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.48-1.68 (m, 4H), 1.69-1.86 (m, 2H), 3.52-3.60 (m, 1H), 3.68-3.84 (m, 2H), 4.02 (ddd, J=10.7, 11.5, 14.5 Hz, 1H), 4.63-4.76 (m, 3H).

步骤3：2-(N-(2，2-二氟-3-((四氢-2H-吡喃-2-基)氧基)丙基)-S-甲基磺酰亚胺基)-2-甲基丙腈(Int-43)Step 3: 2-(N-(2,2-difluoro-3-((tetrahydro-2H-pyran-2-yl)oxy)propyl)-S-methylsulfonylimino)-2-methylpropionitrile (Int-43)

将氢化钾(30％w/w于矿物油中，700mg，5.24mmol)悬浮在1，2-二甲氧基乙烷(12mL)中并且将悬浮液冷却至0-5℃(冰浴)。经5min逐滴加入2-甲基-2-(甲基磺酰亚胺基)丙腈(Int-5，650mg，4.45mmol)在1，2-二甲氧基乙烷(5mL)中的溶液。去除冰浴并且将混合物在室温搅拌30min。之后，将其再次冷却至0-5℃并且加入四丁基溴化铵(80mg，248μmol)，接着加入三氟甲磺酸2，2-二氟-3-羟基丙酯(Int-42，1.53g，4.66mmol)在1，2-二甲氧基乙烷(5mL)中的溶液。将反应混合物在室温搅拌16h。然后，将混合物倒入饱和碳酸氢钠水溶液(15mL)中并用叔丁基甲醚(60mL)稀释。相分离后，将水相用叔丁基甲醚(2x30mL)洗涤，将合并的有机萃取物干燥(硫酸钠)并在真空中浓缩，得到粗产物。将其通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度40∶60至60∶40洗脱)纯化，在真空中干燥(40℃，5毫巴)后，得到标题化合物，为非对映异构体的混合物，为浅黄色粘性油状物(980mg，68％)。HPLC(方法LCMS_梯度)t_R＝2.0min。¹H NMR(CDCl₃，400MHz)：δ1.49-1.87(m，6H)，1.78(s，6H)，3.11&3.12(2s，3H，非对映异构)，3.50-3.77(m，4H)，3.83-4.03(m，2H)，4.72(t，J＝3.2Hz，1H)。MS(ES+)m/z241.1[M+H-(C₅H₈O)]。Potassium hydride (30% w / w in mineral oil, 700 mg, 5.24 mmol) is suspended in 1,2-dimethoxyethane (12 mL) and the suspension is cooled to 0-5 ° C (ice bath). A solution of 2-methyl-2-(methylsulfonylimino)propionitrile (Int-5, 650 mg, 4.45 mmol) in 1,2-dimethoxyethane (5 mL) is added dropwise over 5 min. The ice bath is removed and the mixture is stirred at room temperature for 30 min. Afterwards, it is cooled to 0-5 ° C again and tetrabutylammonium bromide (80 mg, 248 μmol) is added, followed by a solution of 2,2-difluoro-3-hydroxypropyl trifluoromethanesulfonate (Int-42, 1.53 g, 4.66 mmol) in 1,2-dimethoxyethane (5 mL). The reaction mixture is stirred at room temperature for 16 h. The mixture was then poured into a saturated aqueous sodium bicarbonate solution (15 mL) and diluted with tert-butyl methyl ether (60 mL). After phase separation, the aqueous phase was washed with tert-butyl methyl ether (2x30 mL), the combined organic extracts were dried (sodium sulfate) and concentrated in vacuo to give a crude product. The product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 40: 60 to 60: 40), dried in vacuo (40 ° C, 5 mbar), and the title compound was obtained as a mixture of diastereomers, a light yellow viscous oil (980 mg, 68%). HPLC (method LCMS_ gradient) t _R = 2.0 min. ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.49-1.87 (m, 6H), 1.78 (s, 6H), 3.11 & 3.12 (2s, 3H, diastereomers), 3.50-3.77 (m, 4H), 3.83-4.03 (m, 2H), 4.72 (t, J=3.2 Hz, 1H). MS (ES+) m/z 241.1 [M+H-(C ₅ H ₈ O)].

Int-45A和Int-45B：2-((R，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-(2，2-二氟-3-羟基丙基)丙基磺酰亚胺基)-2-甲基丙腈(Int-45A)和2-((S，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-(2，2-二氟-3-羟基丙基)丙基磺酰亚胺基)-2-甲基丙腈(Int-45B)的合成Int-45A and Int-45B: Synthesis of 2-((R,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-(2,2-difluoro-3-hydroxypropyl)propylsulfonylimido)-2-methylpropionitrile (Int-45A) and 2-((S,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-(2,2-difluoro-3-hydroxypropyl)propylsulfonylimido)-2-methylpropionitrile (Int-45B)

步骤1：N-(2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-(2-氰基-N-(2，2-二氟-3-((四氢-2H-吡喃-2-基)氧基)丙基)丙-2-基磺酰亚胺基)丙-2-基)-2-甲基丙-2-亚磺酰胺(Int-44(混合物))Step 1: N-(2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-(2-cyano-N-(2,2-difluoro-3-((tetrahydro-2H-pyran-2-yl)oxy)propyl)propan-2-ylsulfonylimino)propan-2-yl)-2-methylpropane-2-sulfinamide (Int-44 (mixture))

将2-(N-(2，2-二氟-3-((四氢-2H-吡喃-2-基)氧基)丙基)-S-甲基磺酰亚胺基)-2-甲基丙腈(Int-43，960mg，2.96mmol)溶解在THF(8mL)中并且将溶液冷却至＜-70℃(丙酮/干冰浴)。经10min逐滴加入正丁基锂(1.6M于己烷中，1.8mL，2.88mmol)，并且将得到的溶液在＜-70℃搅拌50min。然后，经5min在该温度加入(R，E)-N-(1-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)亚乙基)-2-甲基丙-2-亚磺酰胺(Int-9，1.0g，2.3mmol)在THF(6.0mL)中的溶液。在＜-70℃搅拌30min后，通过tlc和HLPC，反应不完全。经5min加入六甲基二硅基氨基锂在THF中的溶液(1M，2.9mL，2.9mmol)并且将反应混合物在＜-70℃搅拌另外15min。之后，将其倒入2M氯化铵水溶液(20mL)中并用叔丁基甲醚(1x80mL，1x40mL)萃取。将合并的有机萃取物在真空中浓缩，得到黄色的粘性油状物(2.1g)，其在不进一步纯化的情况下用于下一步骤。2-(N-(2,2-difluoro-3-((tetrahydro-2H-pyran-2-yl)oxy)propyl)-S-methylsulfonylimide)-2-methylpropionitrile (Int-43, 960 mg, 2.96 mmol) was dissolved in THF (8 mL) and the solution was cooled to <-70°C (acetone/dry ice bath). n-Butyllithium (1.6 M in hexanes, 1.8 mL, 2.88 mmol) was added dropwise over 10 min, and the resulting solution was stirred at <-70°C for 50 min. Then, a solution of (R,E)-N-(1-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)ethylidene)-2-methylpropane-2-sulfenamide (Int-9, 1.0 g, 2.3 mmol) in THF (6.0 mL) was added at this temperature over 5 min. At＜-70 ℃ of stirring for 30min, by tlc and HLPC, the reaction was incomplete.Through 5min, a solution of lithium hexamethyldisilazide in THF (1M, 2.9mL, 2.9mmol) was added and the reaction mixture was stirred for another 15min at＜-70 ℃.Afterwards, it was poured into 2M aqueous ammonium chloride solution (20mL) and extracted with tert-butyl methyl ether (1x80mL, 1x40mL).The organic extracts merged were concentrated in vacuo to obtain a yellow viscous oil (2.1g), which was used for the next step without further purification.

步骤2：2-((R，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-(2，2-二氟-3-羟基丙基)丙基磺酰亚胺基)-2-甲基丙腈(Int-45A)和2-((S，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-(2，2-二氟-3-羟基丙基)丙基磺酰亚胺基)-2-甲基丙腈(Int-45B)Step 2: 2-((R,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-(2,2-difluoro-3-hydroxypropyl)propylsulfonimido)-2-methylpropionitrile (Int-45A) and 2-((S,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-(2,2-difluoro-3-hydroxypropyl)propylsulfonimido)-2-methylpropionitrile (Int-45B)

将N-(2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-(2-氰基-N-(2，2-二氟-3-((四氢-2H-吡喃-2-基)氧基)丙基)丙-2-基磺酰亚胺基)丙-2-基)-2-甲基-丙-2-亚磺酰胺(Int-44(混合物)，2.10g，2.76mmol)溶解在乙醇(21mL)中，并且在0-5℃(冰浴)加入氯化氢的甲醇溶液(约20％w/w，8.5g，8.5mL，46.6mmol)。将混合物搅在室温拌30min。之后，将其倒到2M碳酸钠水溶液(100mL)上并且用MTBE(150mL)稀释。将得到的悬浮液过滤，将残留物用MTBE(50mL)洗涤。分离合并的滤液的相，将水相用乙酸乙酯(2x50mL)萃取，将合并的有机萃取物干燥(硫酸钠)并在真空中浓缩，得到褐色油状物，为粗产物。将粗产物通过柱色谱(硅胶，100g，用乙酸乙酯/甲醇，梯度100∶0至90∶10洗脱)纯化，在真空中干燥(40℃，5毫巴)，得到标题化合物Int-45A，为单个非对映异构体并且为无色油状物600mg，经两个步骤39％)。HPLC(方法LCMS_梯度)t_R＝2.2min。¹H NMR(CDCl₃，400MHz)：δ0.82-0.90(m，6H)，0.94-1.00(m，9H)，1.59(s，3H)，1.72(s，3H)，1.73(s，3H)，2.86(br s，3H)，3.46(ddd，J＝10.1，13.0，13.0Hz，1H)，3.60(ddd，J＝9.1，13.4，19.1Hz，1H)，3.75-3.85(m，3H)，4.25(d，J＝13.7Hz，1H)，7.36(d，J＝2.7Hz，1H)。MS(ES+)m/z573.1&571.2[M+H，Br]。未分离较少的非对映异构体Int-45B。N-(2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-(2-cyano-N-(2,2-difluoro-3-((tetrahydro-2H-pyran-2-yl)oxy)propyl)propan-2-ylsulfonylimino)propan-2-yl)-2-methyl-propane-2-sulfinamide (Int-44 (mixture), 2.10 g, 2.76 mmol) was dissolved in ethanol (21 mL) and a methanolic solution of hydrogen chloride (approximately 20% w/w, 8.5 g, 8.5 mL, 46.6 mmol) was added at 0-5° C. (ice bath). The mixture was stirred at room temperature for 30 min. Afterwards, it was poured onto a 2M aqueous sodium carbonate solution (100 mL) and diluted with MTBE (150 mL). The resulting suspension was filtered and the residue was washed with MTBE (50 mL). The phases of the combined filtrates were separated, the aqueous phase was extracted with ethyl acetate (2x50 mL), and the combined organic extracts were dried (sodium sulfate) and concentrated in vacuo to give a brown oil as the crude product. The crude product was purified by column chromatography (silica gel, 100 g, eluted with ethyl acetate/methanol, gradient 100:0 to 90:10) and dried in vacuo (40°C, 5 mbar) to give the title compound Int-45A as a single diastereomer and as a colorless oil (600 mg, 39% over two steps). HPLC (Method LCMS_Gradient) _tR = 2.2 min. ¹ H NMR (CDCl ₃ , 400MHz): δ0.82-0.90 (m, 6H), 0.94-1.00 (m, 9H), 1.59 (s, 3H), 1.72 (s, 3H), 1.73 (s, 3H), 2.86 (br s, 3H), 3.46 (ddd, J=10.1, 13.0, 13.0Hz, 1H), 3.60 (ddd, J=9.1, 13.4, 19.1 Hz, 1H), 3.75-3.85 (m, 3H), 4.25 (d, J=13.7Hz, 1H), 7.36 (d, J=2.7Hz, 1H). MS(ES+)m/z573.1&571.2[M+H, Br]. The minor diastereomer, Int-45B, was not separated.

Int-48A：三氟甲磺酸3-(((1R，3R)-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5-((叔丁氧基羰基)氨基)-3，6，6-三甲基-1-氧代-3，6-二氢-2H-1，4-噻嗪-1-亚基)氨基)-2，2-二氟丙酯的合成Int-48A: Synthesis of 3-(((1R,3R)-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5-((tert-butoxycarbonyl)amino)-3,6,6-trimethyl-1-oxo-3,6-dihydro-2H-1,4-thiazin-1-ylidene)amino)-2,2-difluoropropyl trifluoromethanesulfonate

步骤1：(1R，3R)-5-氨基-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((2，2-二氟-3-羟基丙基)亚氨基)-3，6，6-三甲基-3，6-二氢-2H-1，4-噻嗪1-氧化物(Int-46A)Step 1: (1R,3R)-5-amino-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((2,2-difluoro-3-hydroxypropyl)imino)-3,6,6-trimethyl-3,6-dihydro-2H-1,4-thiazine 1-oxide (Int-46A)

将2-(2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-(2，2-二氟-3-羟基丙基)丙基磺酰亚胺基)-2-甲基丙腈(Int-45A，600mg，1.05mmol)溶解在乙醇(9mL)中并加入溴化铜(I)(160mg，1.12mmol)。将混合物加热至75-80℃并且在该温度搅拌45min。之后，将其冷却至5℃(冰浴)并且倒入盐水(10mL)和氨水(25％w/w，5mL)的混合物中。将得到的混合物用MTBE(60mL)和乙酸乙酯(2x25mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩，得到黄色油状物，为粗产物。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/甲醇，梯度95∶5至85∶15洗脱)纯化，在真空中干燥(60℃，5毫巴)后，得到标题化合物，为白色泡沫(490mg，82％)。HPLC(方法LCMS_梯度)t_R＝2.1min。¹H NMR(CDCl₃，400MHz)：δ0.80-0.90(m，6H)，0.93-1.00(m，9H)，1.65(s，3H)，1.73(s，3H)，1.75(s，3H)，2.96-3.09(m，1H)，3.40(d，J＝15.0Hz，1H)，3.40-3.52(m，1H)，3.64-3.80(m，2H)，4.30(d，J＝14.8Hz，1H)，7.33(d，J＝2.4Hz，1H)。MS(ES+)m/z 573.1&571.2[M+H，Br]。2-(2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-(2,2-difluoro-3-hydroxypropyl)propylsulfonylimino)-2-methylpropionitrile (Int-45A, 600 mg, 1.05 mmol) was dissolved in ethanol (9 mL) and copper (I) bromide (160 mg, 1.12 mmol) was added. The mixture was heated to 75-80 ° C and stirred at this temperature for 45 min. Afterwards, it was cooled to 5 ° C (ice bath) and poured into a mixture of brine (10 mL) and ammonia (25% w / w, 5 mL). The resulting mixture was extracted with MTBE (60 mL) and ethyl acetate (2x25 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo to give a yellow oil as a crude product. The crude product was purified by column chromatography (silica gel, 50 g, elution with ethyl acetate/methanol, gradient 95:5 to 85:15) to give, after drying in vacuo (60° C., 5 mbar), the title compound as a white foam (490 mg, 82%). HPLC (method LCMS_Gradient) t _R =2.1 min. ¹ H NMR (CDCl ₃ , 400MHz): δ0.80-0.90 (m, 6H), 0.93-1.00 (m, 9H), 1.65 (s, 3H), 1.73 (s, 3H), 1.75 (s, 3H), 2.96-3.09 (m, 1H), 3.40 (d, J=15.0Hz, 1H), 3.40-3.52 (m, 1H), 3.64-3.80 (m, 2H), 4.30 (d, J=14.8Hz, 1H), 7.33 (d, J=2.4Hz, 1H). MS(ES+)m/z 573.1&571.2[M+H, Br].

步骤2：((1R，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((2，2-二氟-3-羟基丙基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-47A)Step 2: tert-Butyl ((1R,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((2,2-difluoro-3-hydroxypropyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-47A)

将(1R，3R)-5-氨基-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((2，2-二氟-3-羟基丙基)亚氨基)-3，6，6-三甲基-3，6-二氢-2H-1，4-噻嗪1-氧化物(Int-46A，490mg，857μmol)溶解在THF(5mL)和水(1mL)中并且加入Boc-酐(280mg，298μL，1.28mmol)，碳酸氢钠(100mg，1.19mmol)和4-二甲基氨基吡啶(5mg，41μmol)。将混合物在室温搅拌3.75h。之后，加入氨水(25％w/w，90mg，100μL，1.32mmol)并且将混合物搅拌另外30min。然后将反应混合物倒到水(10mL)上并且用MTBE(1x80mL，1x40mL)萃取。将合并的萃取物干燥并在真空中浓缩。将粗产物通过柱色谱(硅胶，100g，用乙酸乙酯/正庚烷，梯度40∶60至60∶40洗脱)纯化，在真空中干燥(55℃，5毫巴)后得到标题化合物，为白色泡沫(520mg，90％)。HPLC(方法LCMS_梯度)t_R＝4.1min。¹H NMR(CDCl₃，400MHz)：δ0.82-0.90(m，6H)，0.93-0.99(m，9H)，1.54(s，9H)，1.64(s，3H)，1.78(s，3H)，1.83(s，3H)，2.66(t，J＝7.5Hz，1H)，2.86(ddd，J＝10.5，13.2，13.2Hz，1H)，3.42(ddd，J＝8.9，13.4，18.5Hz，1H)，3.57(d，J＝15.3Hz，1H)，3.60-3.80(m，2H)，4.56(d，J＝15.0Hz，1H)，7.38(d，J＝2.7Hz，1H)，11.15(s，1H)。MS(ES+)m/z 673.2&671.2[M+H，Br]。(1R,3R)-5-amino-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((2,2-difluoro-3-hydroxypropyl)imino)-3,6,6-trimethyl-3,6-dihydro-2H-1,4-thiazine 1-oxide (Int-46A, 490 mg, 857 μmol) was dissolved in THF (5 mL) and water (1 mL) and Boc-anhydride (280 mg, 298 μL, 1.28 mmol), sodium bicarbonate (100 mg, 1.19 mmol) and 4-dimethylaminopyridine (5 mg, 41 μmol) were added. The mixture was stirred at room temperature for 3.75 h. Afterwards, aqueous ammonia (25% w/w, 90 mg, 100 μL, 1.32 mmol) was added and the mixture was stirred for another 30 min. The reaction mixture was then poured onto water (10 mL) and extracted with MTBE (1 x 80 mL, 1 x 40 mL). The combined extracts were dried and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 100 g, eluted with ethyl acetate/n-heptane, gradient 40:60 to 60:40) and dried in vacuo (55° C., 5 mbar) to give the title compound as a white foam (520 mg, 90%). HPLC (Method LCMS_Gradient) t _R = 4.1 min. ¹ H NMR (CDCl ₃ , 400MHz): δ0.82-0.90 (m, 6H), 0.93-0.99 (m, 9H), 1.54 (s, 9H), 1.64 (s, 3H) , 1.78 (s, 3H), 1.83 (s, 3H), 2.66 (t, J=7.5Hz, 1H), 2.86 (ddd, J=10.5, 13.2, 1 3.2Hz, 1H), 3.42 (ddd, J=8.9, 13.4, 18.5Hz, 1H), 3.57 (d, J=15.3Hz, 1H), 3.6 0-3.80 (m, 2H), 4.56 (d, J=15.0Hz, 1H), 7.38 (d, J=2.7Hz, 1H), 11.15 (s, 1H). MS(ES+)m/z 673.2&671.2[M+H, Br].

步骤3：三氟甲磺酸3-(((1R，3R)-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5-((叔丁氧基羰基)氨基)-3，6，6-三甲基-1-氧代-3，6-二氢-2H-1，4-噻嗪-1-亚基)氨基)-2，2-二氟丙酯(Int-48A)Step 3: 3-(((1R,3R)-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5-((tert-butoxycarbonyl)amino)-3,6,6-trimethyl-1-oxo-3,6-dihydro-2H-1,4-thiazin-1-ylidene)amino)-2,2-difluoropropyl trifluoromethanesulfonate (Int-48A)

将((1R，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-((2，2-二氟-3-羟基丙基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-47A，2.05g，3.05mmol)溶解在二氯甲烷(25mL)中，将溶液冷却至0-5℃(冰浴)并且接着在该温度加入吡啶(302mg，309μL，3.82mmol)以及三氟甲磺酸酐(947mg，3.36mmol)在二氯甲烷(8ml)中的溶液。将得到的黄色溶液在0-5℃搅拌30min。之后，加入碳酸氢钠水溶液(5％，100mL)并且证实水相的pH为8-9。分离相并且将水相用二氯甲烷(2x60mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，40g，用乙酸乙酯/正庚烷，梯度5∶95至30∶70洗脱)纯化，在真空中干燥(40℃，5毫巴)得到标题化合物，为浅黄色泡沫(2.15g，88％)。HPLC(方法LCMS_fglm)t_R＝1.86min。¹H NMR(CDCl₃，300MHz)：δ0.81-0.91(m，6H)，0.93-1.01(m，9H)，1.55(s，9H)，1.62(s，3H)，1.78(s，3H)，1.83(s，3H)，3.07(ddd，J＝9.9，13.9，13.9Hz，1H)，3.44(ddd，J＝9.5，13.5，17.1Hz，1H)，3.57(d，J＝15.1Hz，1H)，4.46-4.56(m，2H)，4.56(d，J＝15.1Hz，1H)，7.39(d，J＝2.6Hz，1H)，11.20(s，1H)。MS(ES+)m/z 803.2&805.2[M+H，Br]。Tert-butyl ((1R,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-((2,2-difluoro-3-hydroxypropyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-47A, 2.05 g, 3.05 mmol) was dissolved in dichloromethane (25 mL), the solution was cooled to 0-5° C. (ice bath) and then pyridine (302 mg, 309 μL, 3.82 mmol) and a solution of trifluoromethanesulfonic anhydride (947 mg, 3.36 mmol) in dichloromethane (8 ml) were added at this temperature. The resulting yellow solution was stirred at 0-5° C. for 30 min. Afterwards, aqueous sodium bicarbonate solution (5%, 100 mL) was added and the pH of the aqueous phase was confirmed to be 8-9. The phases were separated and the aqueous phase was extracted with dichloromethane (2x60 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo to give a crude product. The crude product was purified by column chromatography (silica gel, 40 g, eluted with ethyl acetate/n-heptane, gradient 5: 95 to 30: 70) and dried in vacuo (40° C., 5 mbar) to give the title compound as a light yellow foam (2.15 g, 88%). HPLC (method LCMS_fglm) t _R =1.86 min. ¹ H NMR (CDCl ₃ , 300MHz): δ0.81-0.91 (m, 6H), 0.93-1.01 (m, 9H), 1.55 (s, 9H), 1.62 (s, 3H), 1.78 (s, 3H), 1.83 (s, 3H), 3.07 (ddd, J=9.9, 13.9, 13.9Hz, 1H), 3.44 (ddd, J=9.5, 13.5, 17.1Hz, 1H), 3.57 (d, J=15.1Hz, 1H), 4.46-4.56 (m, 2H), 4.56 (d, J=15.1Hz, 1H), 7.39 (d, J=2.6Hz, 1H), 11.20 (s, 1H). MS(ES+)m/z 803.2&805.2[M+H, Br].

Int-49AA和Int-49AB：((4aR，5R，9R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-49AA)和((4aS，5R，9R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-49AB)的合成Int-49AA and Int-49AB: tert-Butyl ((4aR,5R,9R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-49AA and Int-49AB): tert-Butyl ((4aR,5R,9R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate Synthesis of tert-butyl ((4aS,5R,9R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-49AB)

在无水条件下将三氟甲磺酸3-(((1R，3R)-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5-((叔丁氧基羰基)氨基)-3，6，6-三甲基-1-氧代-3，6-二氢-2H-1，4-噻嗪-1-亚基)氨基)-2，2-二氟丙酯(Int-48A，2.13g，2.65mmol)溶解在THF(25mL)中并且将溶液冷却至＜-70℃(丙酮/干冰浴)。经15min加入LHMDS在THF中的溶液(1.0M，6.89ml，6.89mmol)并且在该温度搅拌15min。然后，将得到的澄清，黄色溶液加温至-20℃(冰/乙醇浴)并在该温度搅拌90min，TLC显示完全转化。通过加入饱和氯化铵水溶液(60mL)终止反应，并且在相分离后，将水相用乙酸乙酯(2x60mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，40g，用乙酸乙酯/正庚烷，梯度5∶95至35∶65洗脱)纯化，在真空中干燥(55℃，5毫巴)后，得到标题化合物Int-49AA，为单个非对映异构体并且为无色粘性油状物(1.57g，91％)。HPLC(方法LCMS_梯度)t_R＝4.5min。¹H NMR(CDCl₃，400MHz)：δ0.83-0.91(m，6H)，0.94-1.00(m，9H)，1.47(s，9H)，1.79(s，3H)，1.82(s，3H)，1.93(br s，3H)，2.55-2.77(m，2H)，3.63-3.86(m，2H)，4.50-4.57(m，1H)，7.45(d，J＝2.7Hz，1H)，11.45(s，1H)。MS(ES+)m/z 555.1&553.1[M+H-CO₂-CH₂＝CMe₂，Br]。较少的非对映异构体未分离。3-(((1R,3R)-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5-((tert-butoxycarbonyl)amino)-3,6,6-trimethyl-1-oxo-3,6-dihydro-2H-1,4-thiazin-1-ylidene)amino)-2,2-difluoropropyl trifluoromethanesulfonate (Int-48A, 2.13 g, 2.65 mmol) was dissolved in THF (25 mL) under anhydrous conditions and the solution was cooled to <-70°C (acetone/dry ice bath). A solution of LHMDS in THF (1.0 M, 6.89 ml, 6.89 mmol) was added over 15 min and stirred at this temperature for 15 min. The resulting clear, yellow solution was then warmed to -20°C (ice/ethanol bath) and stirred at this temperature for 90 min. TLC showed complete conversion. The reaction was terminated by adding saturated aqueous ammonium chloride solution (60 mL), and after phase separation, aqueous phase was extracted with ethyl acetate (2x60 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 40 g, eluted with ethyl acetate/n-heptane, gradient 5: 95 to 35: 65), dried in vacuo (55 ° C, 5 mbar), to obtain the title compound Int-49AA, which is a single diastereomer and a colorless viscous oil (1.57 g, 91%). HPLC (method LCMS_ gradient) t _R = 4.5 min. ^1H NMR (CDCl ₃ , 400 MHz): δ 0.83-0.91 (m, 6H), 0.94-1.00 (m, 9H), 1.47 (s, 9H), 1.79 (s, 3H), 1.82 (s, 3H), 1.93 (br s, 3H), 2.55-2.77 (m, 2H), 3.63-3.86 (m, 2H), 4.50-4.57 (m, 1H), 7.45 (d, J=2.7 Hz, 1H), 11.45 (s, 1H). MS (ES+) m/z 555.1 & 553.1 [M+H-CO ₂ -CH ₂ =CMe ₂ , Br]. The minor diastereomer was not separated.

Int-51AA：((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯的合成Int-51AA: Synthesis of tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate

步骤1：((4aR，5R，9R)-5-(6-溴-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-50AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-bromo-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-50AA)

将((4aR，5R，9R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-49AA，170mg，260μmol)溶解在THF(4mL)和DMF(1mL)中。在室温加入乙酸(52.5mg，50μl，873μmol)和氟化钾(50mg，861μmol)并且在该温度将得到的精细悬浮液搅拌2.5h。之后，将其倒入饱和碳酸氢钠水溶液(10mL)中并用MTBE(1x60mL，1x30mL)萃取。将合并的萃取物用盐水(10mL)洗涤，干燥(硫酸钠)并在真空中浓缩。将残留物用邻二甲苯(8mL)处理并再次在真空中浓缩，得到黄色粘性油状物，为粗产物。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度20∶80至40∶60洗脱)纯化，在真空中干燥(55℃，5毫巴)后得到标题化合物，为白色泡沫(130mg，93％)。HPLC(方法LCMS_梯度)t_R＝3.1min。¹H NMR(CDCl₃，400MHz)：δ1.49(s，9H)，1.80(s，3H)，1.82(s，3H)，1.93(d，J＝1.1Hz，3H)，2.49-2.60(m，1H)，2.61-2.78(m，1H)，3.63-3.86(m，2H)，4.45-4.51(m，1H)，7.41(dd，J＝8.6，10.7Hz，1H)，7.54(dd，J＝3.1，8.5Hz，1H)，11.31(s，1H)。MS(ES+)m/z 541.1&539.1[M+H，Br]。Tert-butyl ((4aR, 5R, 9R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-49AA, 170 mg, 260 μmol) was dissolved in THF (4 mL) and DMF (1 mL). Acetic acid (52.5 mg, 50 μl, 873 μmol) and potassium fluoride (50 mg, 861 μmol) were added at room temperature and the resulting fine suspension was stirred at this temperature for 2.5 h. Afterwards, it was poured into saturated aqueous sodium bicarbonate solution (10 mL) and extracted with MTBE (1 x 60 mL, 1 x 30 mL). The combined extracts were washed with brine (10 mL), dried (sodium sulfate) and concentrated in vacuo. The residue was treated with o-xylene (8 mL) and concentrated again in vacuo to give a yellow viscous oil as the crude product. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 20: 80 to 40: 60) and dried in vacuo (55° C., 5 mbar) to give the title compound as a white foam (130 mg, 93%). HPLC (method LCMS_gradient) t _R = 3.1 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.49 (s, 9H), 1.80 (s, 3H), 1.82 (s, 3H), 1.93 (d, J = 1.1Hz, 3H), 2.49-2.60 (m, 1H), 2.61-2.78 (m, 1H) , 3.63-3.86 (m, 2H), 4.45-4.51 (m, 1H), 7.41 (dd, J=8.6, 10.7Hz, 1H), 7.54 (dd, J=3.1, 8.5Hz, 1H), 11.31 (s, 1H). MS(ES+)m/z 541.1&539.1[M+H, Br].

步骤2：((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AA)Step 2: tert-Butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AA)

将((4aR，5R，9R)-5-(6-溴-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-50AA，120mg，222μmol)溶解在1，4-二烷(3mL)和水(1mL)中并且加入叠氮化钠(200mg，3.08mmol)。接着加入碘化酮(I)(30mg，158μmol)，抗坏血酸钠(15mg，76μmol)和反式-N，N′-二甲基-1，2-环己烷二胺(36.1mg，40μl，254μmol)并且将深蓝色混合物在70℃搅拌1h。因为转化不完全，接着加入另一部份的叠氮化钠(100mg，1.54mmol)，碘化酮(I)(30mg，158μmol)，抗坏血酸钠(15mg，76μmol)和反式-N，N′-二甲基-1，2-环己烷二胺(36.1mg，40μl，254μmol)并且将混合物在70℃搅拌另外30min。然后，将反应混合物冷却至室温并倒入饱和碳酸氢钠水溶液(10mL)中。将其用乙酸乙酯(1x50mL，2x30mL)萃取，将合并的萃取物用盐水(10mL)和氨(25％w/w，1mL)的混合物洗涤，经硫酸钠干燥并将硅胶(2g)加入至溶液中。过滤后，将滤液在真空中浓缩，得到黄色固体，为粗产物。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度30∶70至50∶50洗脱)纯化，在真空中干燥(55℃，5毫巴)后得到标题化合物，为白色固体(70mg，66％)。HPLC(方法LCMS_梯度)t_R＝2.6min。¹H NMR(CDCl₃，400MHz)：δ1.49(s，9H)，1.78(s，3H)，1.80(s，3H)，1.87(d，J＝1.6Hz，3H)，2.58-2.75(m，2H)，3.62-3.86(m，2H)，4.41(s，2H)，4.59-4.65(m，1H)，6.49(dd，J＝2.6，8.6Hz，1H)，7.28(dd，J＝8.7，10.9Hz，1H)，11.23(s，1H)。MS(ES+)m/z 476.2[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-bromo-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-50AA, 120 mg, 222 μmol) was dissolved in 1,4-dioxane (3 mL) and water (1 mL) and sodium azide (200 mg, 3.08 mmol) was added. Iodide ketone (I) (30 mg, 158 μmol), sodium ascorbate (15 mg, 76 μmol) and trans-N,N′-dimethyl-1,2-cyclohexanediamine (36.1 mg, 40 μl, 254 μmol) were then added and the dark blue mixture was stirred at 70° C. for 1 h. Because the conversion was incomplete, another portion of sodium azide (100 mg, 1.54 mmol), iodine ketone (I) (30 mg, 158 μmol), sodium ascorbate (15 mg, 76 μmol) and trans-N, N'-dimethyl-1,2-cyclohexanediamine (36.1 mg, 40 μl, 254 μmol) were added and the mixture was stirred at 70 ° C for another 30 min. The reaction mixture was then cooled to room temperature and poured into saturated aqueous sodium bicarbonate solution (10 mL). It was extracted with ethyl acetate (1x50 mL, 2x30 mL), and the combined extracts were washed with a mixture of brine (10 mL) and ammonia (25% w/w, 1 mL), dried over sodium sulfate and silica gel (2 g) was added to the solution. After filtration, the filtrate was concentrated in vacuo to give a yellow solid as a crude product. The crude product was purified by column chromatography (silica gel, 50 g, elution with ethyl acetate/n-heptane, gradient 30:70 to 50:50) to afford after drying in vacuo (55° C., 5 mbar) the title compound as a white solid (70 mg, 66%). HPLC (method LCMS_Gradient) t _R =2.6 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.49 (s, 9H), 1.78 (s, 3H), 1.80 (s, 3H), 1.87 (d, J = 1.6Hz, 3H), 2.58-2.75 (m, 2H), 3.62-3.86 (m, 2 H), 4.41 (s, 2H), 4.59-4.65 (m, 1H), 6.49 (dd, J=2.6, 8.6Hz, 1H), 7.28 (dd, J=8.7, 10.9Hz, 1H), 11.23 (s, 1H). MS(ES+)m/z 476.2[M+H].

Int-51AAp：对映异构纯((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯的合成Int-51AAp: Synthesis of enantiomerically pure tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate

((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AA，1.68g，3.53mmol)的对映异构纯化通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/乙醇90/10，流速1.0mL/min)进行，得到为白色固体的所需(-)-旋光首先洗脱对映异构体(Int-51AAp，979mg，58％)，和为白色固体的相反(+)-旋光对映异构体(Int-51AAq，366mg，22％)。Enantiomeric purification of tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AA, 1.68 g, 3.53 mmol) was performed by chiral preparative HPLC (Chiralpak AD, 250*4.6mm*5μm, isocratic, n-heptane/ethanol 90/10, flow rate 1.0mL/min) to give the desired (-)-optically active first eluting enantiomer (Int-51AAp, 979mg, 58%) as a white solid, and the opposite (+)-optically active enantiomer (Int-51AAq, 366mg, 22%) as a white solid.

Int-55：2-甲基-2-(S-甲基-N-((1-(((四氢-2H-吡喃-2-基)氧基)甲基)环丙基)甲基)磺酰亚胺基)丙腈的合成Int-55: Synthesis of 2-methyl-2-(S-methyl-N-((1-(((tetrahydro-2H-pyran-2-yl)oxy)methyl)cyclopropyl)methyl)sulfonylimino)propionitrile

步骤1：(1-(((四氢-2H-吡喃-2-基)氧基)甲基)环丙基)甲醇(Int-53)Step 1: (1-(((Tetrahydro-2H-pyran-2-yl)oxy)methyl)cyclopropyl)methanol (Int-53)

在30℃将环丙-1，1-二基二甲醇(16.0g，157mmol)悬浮在甲苯(312mL)中，加入2，3-二氢吡喃(43.6g，47.0ml，519mmol)，接着加入硫酸氢钠水溶液(5mol/L，31.6mL，158mmol)并且将两相混合物在30℃搅拌1h。之后，加入固体碳酸钠(140g，1.33mol)，将得到的悬浮液在室温搅拌30min并且过滤。将沉淀用甲苯(4x125mL)洗涤，并且将合并的滤液在真空中浓缩(40℃/5毫巴)。将粗产物通过柱色谱(硅胶，330g，用叔丁基甲醚/正庚烷，梯度20∶80至35∶65洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为浅黄色液体(21.1g，72％)。¹H NMR(CDCl₃，300MHz)：δ0.48-0.58(m，4H)，1.51-1.64(m，4H)，1.72-1.78(m，1H)，1.79-1.87(m，1H)，2.63(br s，1H)，3.40(d，J＝10.3Hz，1H)，3.49-3.55(m，1H)，3.51(d，J＝11.3Hz，1H)，3.63(d，J＝11.2Hz，1H)，3.76(d，J＝10.2Hz，1H)，3.87-3.92(m，1H)，4.61-4.63(m，1H)。Cyclopropane-1,1-diyldimethanol (16.0 g, 157 mmol) was suspended in toluene (312 mL) at 30° C. 2,3-dihydropyran (43.6 g, 47.0 ml, 519 mmol) was added, followed by aqueous sodium bisulfate (5 mol/L, 31.6 mL, 158 mmol) and the two-phase mixture was stirred at 30° C. for 1 h. Afterwards, solid sodium carbonate (140 g, 1.33 mol) was added, the resulting suspension was stirred at room temperature for 30 min and filtered. The precipitate was washed with toluene (4 x 125 mL) and the combined filtrates were concentrated in vacuo (40° C./5 mbar). The crude product was purified by column chromatography (silica gel, 330 g, eluted with tert-butyl methyl ether/n-heptane, gradient 20:80 to 35:65) to give the title compound as a light yellow liquid (21.1 g, 72%) after drying in vacuo (40° C., 5 mbar). ¹ H NMR (CDCl ₃ , 300MHz): δ0.48-0.58(m, 4H), 1.51-1.64(m, 4H), 1.72-1.78(m, 1H), 1.79-1.87(m, 1H), 2.63(br s, 1H), 3.40 (d, J = 10.3Hz, 1H), 3.49-3.55 (m, 1H), 3.51 (d, J = 11.3Hz, 1H), 3.63 ( d, J=11.2Hz, 1H), 3.76 (d, J=10.2Hz, 1H), 3.87-3.92 (m, 1H), 4.61-4.63 (m, 1H).

步骤2：2-((1-(碘甲基)环丙基)甲氧基)四氢-2H-吡喃(Int-54)Step 2: 2-((1-(iodomethyl)cyclopropyl)methoxy)tetrahydro-2H-pyran (Int-54)

将2，3-二氯-5，6-二氰基-1，4-苯醌(6.67g，29.4mmol)溶解在二氯甲烷(200mL)中并且加入三苯基膦(7.71g，29.4mmol)。将棕色悬浮液冷却至0-5℃(冰浴)并且以一部分加入四正丁基碘化铵(10.9g，29.4mmol)，接着加入(1-(((四氢-2H-吡喃-2-基)氧基)甲基)环丙基)甲醇(Int-53，5.21g，28mmol)在二氯甲烷(50mL)中的溶液。将反应混合物在0-5℃搅拌15min并且在室温搅拌1h。之后，将其在真空中浓缩并且直接通过柱色谱(硅胶，220g，用乙酸乙酯/正庚烷，梯度2∶98至15∶85洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为无色液体(6.0g，72％)。¹H NMR(CDCl₃，600MHz)：δ0.60-0.72(m，4H)，0.83-0.98(m，2H)，1.48-1.91(m，6H)，3.29(d，AB，J＝9.9Hz，1H)，3.38-3.45(m，2H)，3.49-3.57(m，1H)，3.62(d，AB，J＝10.5Hz，1H)，3.85-3.94(m，1H)，4.64-4.68(m，1H)。2,3-Dichloro-5,6-dicyano-1,4-benzoquinone (6.67 g, 29.4 mmol) was dissolved in dichloromethane (200 mL) and triphenylphosphine (7.71 g, 29.4 mmol) was added. The brown suspension was cooled to 0-5° C. (ice bath) and tetra-n-butylammonium iodide (10.9 g, 29.4 mmol) was added in one portion, followed by a solution of (1-(((tetrahydro-2H-pyran-2-yl)oxy)methyl)cyclopropyl)methanol (Int-53, 5.21 g, 28 mmol) in dichloromethane (50 mL). The reaction mixture was stirred at 0-5° C. for 15 min and at room temperature for 1 h. Afterwards, it was concentrated in vacuo and purified directly by column chromatography (silica gel, 220 g, eluted with ethyl acetate/n-heptane, gradient 2:98 to 15:85) to give, after drying in vacuo (40° C., 5 mbar), the title compound as a colorless liquid (6.0 g, 72%). ¹ H NMR (CDCl ₃ , 600MHz): δ0.60-0.72 (m, 4H), 0.83-0.98 (m, 2H), 1.48-1.91 (m, 6H), 3.29 (d, AB, J = 9.9Hz, 1H), 3.3 8-3.45 (m, 2H), 3.49-3.57 (m, 1H), 3.62 (d, AB, J = 10.5Hz, 1H), 3.85-3.94 (m, 1H), 4.64-4.68 (m, 1H).

步骤3：2-甲基-2-(S-甲基-N-((1-(((四氢-2H-吡喃-2-基)氧基)甲基)环丙基)甲基)磺酰亚胺基)丙腈(Int-55)Step 3: 2-Methyl-2-(S-methyl-N-((1-(((tetrahydro-2H-pyran-2-yl)oxy)methyl)cyclopropyl)methyl)sulfonylimino)propionitrile (Int-55)

将氢化钾(30％w/w于矿物油中，758mg，5.67mmol)悬浮在四氢呋喃(5mL)中并且将悬浮液冷却至0-5℃(冰浴)。经5min逐滴加入2-甲基-2-(甲基磺酰亚胺基)丙腈(Int-5，829mg，5.67mmol)在四氢呋喃(3mL)中的溶液。将混合物在0-5℃搅拌15min并且在室温搅拌90min。之后，将其再次冷却至0-5℃并且加入四丁基溴化铵(45.1mg，140μmol)，接着加入2-((1-(碘甲基)环丙基)甲氧基)四氢-2H-吡喃(Int-54，829mg，2.8mmol)在四氢呋喃(3mL)中的溶液。将反应混合物在室温搅拌14h，接着在65℃(回流)搅拌24h。冷却至室温后，将混合物倒入饱和碳酸氢钠水溶液(5％m/m，40mL)中并用乙酸乙酯(2x50mL)萃取。将合并的有机萃取物干燥(硫酸钠)并在真空中浓缩，得到粗产物。将其通过柱色谱(硅胶，40g，用乙酸乙酯/正庚烷，梯度25∶75至75∶25洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为非对映异构体的混合物，为黄色粘性油状物(404mg，46％)。¹H NMR(CDCl₃，300MHz)：δ0.43-0.58(m，4H)，1.48-1.65(m，4H)，1.68-1.91(m，2H)，1.76(s，6H)，3.08(2s，3H，非对映异构)，3.10-3.54(m，4H)，3.60(dd，J＝6.0，10.2Hz，1H)，3.84-3.93(m，1H)，4.61-4.66(m，1H)。MS(ES+)m/z 315.2[M+H]。Potassium hydride (30% w / w in mineral oil, 758 mg, 5.67 mmol) was suspended in tetrahydrofuran (5 mL) and the suspension was cooled to 0-5 ° C (ice bath). A solution of 2-methyl-2-(methylsulfonylimide)propionitrile (Int-5, 829 mg, 5.67 mmol) in tetrahydrofuran (3 mL) was added dropwise over 5 min. The mixture was stirred at 0-5 ° C for 15 min and at room temperature for 90 min. Afterwards, it was cooled to 0-5 ° C again and tetrabutylammonium bromide (45.1 mg, 140 μmol) was added, followed by a solution of 2-((1-(iodomethyl)cyclopropyl)methoxy)tetrahydro-2H-pyran (Int-54, 829 mg, 2.8 mmol) in tetrahydrofuran (3 mL). The reaction mixture was stirred at room temperature for 14 h, then at 65 ° C (reflux) for 24 h. After cooling to room temperature, the mixture was poured into a saturated aqueous sodium bicarbonate solution (5% m/m, 40 mL) and extracted with ethyl acetate (2x50 mL). The combined organic extracts were dried (sodium sulfate) and concentrated in vacuo to give a crude product. The product was purified by column chromatography (silica gel, 40 g, eluted with ethyl acetate/n-heptane, gradient 25: 75 to 75: 25), dried in vacuo (40° C., 5 mbar) to give the title compound, a mixture of diastereomers, a yellow viscous oil (404 mg, 46%). ¹ H NMR (CDCl ₃ , 300 MHz): δ 0.43-0.58 (m, 4H), 1.48-1.65 (m, 4H), 1.68-1.91 (m, 2H), 1.76 (s, 6H), 3.08 (2s, 3H, diastereoisomers), 3.10-3.54 (m, 4H), 3.60 (dd, J=6.0, 10.2 Hz, 1H), 3.84-3.93 (m, 1H), 4.61-4.66 (m, 1H). MS (ES+) m/z 315.2 [M+H].

Int-57A和Int-57B：2-((R，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-((1-(羟基甲基)环丙基)甲基)丙基磺酰亚胺基)-2-甲基丙腈(Int-57A)和2-((S，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-((1-(羟基甲基)环丙基)甲基)丙基磺酰亚胺基)-2-甲基丙腈(Int-57B)的合成Int-57A and Int-57B: Synthesis of 2-((R,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-((1-(hydroxymethyl)cyclopropyl)methyl)propylsulfonimido)-2-methylpropionitrile (Int-57A) and 2-((S,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-((1-(hydroxymethyl)cyclopropyl)methyl)propylsulfonimido)-2-methylpropionitrile (Int-57B)

步骤1：N-(2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-(2-氰基-N-((1-(((四氢-2H-吡喃-2-基)氧基)甲基)环丙基)甲基)丙-2-基磺酰亚胺基)丙-2-基)-2-甲基丙-2-亚磺酰胺(Int-56(混合物))Step 1: N-(2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-(2-cyano-N-((1-(((tetrahydro-2H-pyran-2-yl)oxy)methyl)cyclopropyl)methyl)propan-2-ylsulfonylimino)propan-2-yl)-2-methylpropane-2-sulfinamide (Int-56 (Mixture))

将2-甲基-2-(S-甲基-N-((1-(((四氢-2H-吡喃-2-基)氧基)甲基)环丙基)甲基)磺酰亚胺基)丙腈(Int-55，2.34g，7.44mmol)溶解在THF(40mL)中并且将溶液冷却至＜-70℃(丙酮/干冰浴)。经10min逐滴加入正丁基锂(1.6M于己烷中，4.65mL，7.44mmol)，并且将得到的溶液在＜-70℃搅拌1h。然后，在该温度经20min加入(R，E)-N-(1-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)亚乙基)-2-甲基丙-2-亚磺酰胺(Int-9，2.7g，6.2mmol)在THF(20mL)中的溶液。在＜-70℃搅拌90min后，加入饱和氯化铵水溶液(100mL)并且将混合物加温至室温。然后，将其用水稀释(50mL)并用乙酸乙酯(2x100mL)萃取。将合并的有机萃取物干燥(硫酸钠)并在真空中浓缩，得到黄色的粘性油状物(6.08g)，其在不进一步纯化的情况下用于下一步骤。2-Methyl-2-(S-methyl-N-((1-(((tetrahydro-2H-pyran-2-yl)oxy)methyl)cyclopropyl)methyl)sulfonylimino)propionitrile (Int-55, 2.34 g, 7.44 mmol) was dissolved in THF (40 mL) and the solution was cooled to <-70°C (acetone/dry ice bath). n-Butyllithium (1.6 M in hexanes, 4.65 mL, 7.44 mmol) was added dropwise over 10 min, and the resulting solution was stirred at <-70°C for 1 h. A solution of (R,E)-N-(1-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)ethylidene)-2-methylpropane-2-sulfenamide (Int-9, 2.7 g, 6.2 mmol) in THF (20 mL) was then added at this temperature over 20 min. After stirring for 90 min at＜-70 ℃, add saturated aqueous ammonium chloride solution (100 mL) and the mixture is warmed to room temperature. Then, it is diluted with water (50 mL) and extracted with ethyl acetate (2x100 mL). The organic extracts merged are dried (sodium sulfate) and concentrated in vacuo to obtain a yellow viscous oil (6.08 g), which is used for the next step without further purification.

步骤2：2-((R，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-((1-(羟基甲基)环丙基)甲基)丙基磺酰亚胺基)-2-甲基丙腈(Int-57A)和2-((S，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-((1-(羟基甲基)环丙基)甲基)丙基磺酰亚胺基)-2-甲基丙腈(Int-57B)Step 2: 2-((R,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-((1-(hydroxymethyl)cyclopropyl)methyl)propylsulfonimido)-2-methylpropionitrile (Int-57A) and 2-((S,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-((1-(hydroxymethyl)cyclopropyl)methyl)propylsulfonimido)-2-methylpropionitrile (Int-57B)

将N-(2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-(2-氰基-N-((1-(((四氢-2H-吡喃-2-基)氧基)甲基)环丙基)甲基)丙-2-基磺酰亚胺基)丙-2-基)-2-甲基丙-2-亚磺酰胺(Int-56(混合物)，6.08g，6.2mmol)溶解在乙醇(25mL)中并且在0-5℃(冰浴)加入氯化氢的甲醇溶液(约20％w/w，41.9g，45.6mL，676mmol)。将混合物在0-5℃搅拌40min。之后，加入碳酸钠水溶液(10％m/m，300mL)，并且将得到的混合物用乙酸乙酯(2x150mL)萃取。将合并的有机萃取物干燥(硫酸钠)并在真空中浓缩，得到黄色油状物，为粗产物。将粗产物通过柱色谱(硅胶，120g，用乙酸乙酯/甲醇，梯度100∶0至90∶10洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物Int-57A，为单个非对映异构体并且为浅黄色固体(1.8g，经2个步骤52％)。较少非对映异构体Int-57B未分离。Int-57A：¹H NMR(CDCl₃，300MHz)：δ0.30-0.50(m，4H)，0.81-0.93(m，6H)，0.94-1.03(m，9H)，1.56(s，3H)，1.67(s，3H)，1.72(s，3H)，2.77(br s，3H)，3.01(d，AB，J＝12.5Hz，1H)，3.14(d，AB，J＝12.3Hz，1H)，3.42(d，AB，J＝11.5Hz，1H)，3.51(d，AB，J＝11.5Hz，1H)，3.77(dd，AB，J＝1.2，13.5Hz，1H)，4.13(d，AB，J＝13.5Hz，1H)，7.36(d，J＝2.6Hz，1H)。MS(ES+)m/z 563.2&561.2[M+H，Br]。N-(2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-(2-cyano-N-((1-(((tetrahydro-2H-pyran-2-yl)oxy)methyl)cyclopropyl)methyl)propan-2-ylsulfonylimino)propan-2-yl)-2-methylpropane-2-sulfinamide (Int-56 (mixture), 6.08 g, 6.2 mmol) was dissolved in ethanol (25 mL) and a methanolic solution of hydrogen chloride (approximately 20% w/w, 41.9 g, 45.6 mL, 676 mmol) was added at 0-5° C. (ice bath). The mixture was stirred at 0-5° C. for 40 min. Afterwards, aqueous sodium carbonate (10% m/m, 300 mL) was added and the resulting mixture was extracted with ethyl acetate (2×150 mL). The combined organic extracts were dried (sodium sulfate) and concentrated in vacuo to give a yellow oil as the crude product. The crude product was purified by column chromatography (silica gel, 120 g, eluted with ethyl acetate/methanol, gradient 100:0 to 90:10) to afford the title compound Int-57A as a single diastereomer after drying in vacuo (40° C., 5 mbar) as a light yellow solid (1.8 g, 52% over 2 steps). The minor diastereomer Int-57B was not separated. Int-57A: ¹ H NMR (CDCl ₃ , 300 MHz): δ 0.30-0.50 (m, 4H), 0.81-0.93 (m, 6H), 0.94-1.03 (m, 9H), 1.56 (s, 3H), 1.67 (s, 3H), 1.72 (s, 3H), 2.77 (br s, 3H), 3.01 (d, AB, J = 12.5Hz, 1H), 3.14 (d, AB, J = 12.3Hz, 1H), 3.42 (d, AB, J = 11.5Hz, 1H), 3.51 (d, AB , J=11.5Hz, 1H), 3.77 (dd, AB, J=1.2, 13.5Hz, 1H), 4.13 (d, AB, J=13.5Hz, 1H), 7.36 (d, J=2.6Hz, 1H). MS(ES+)m/z 563.2&561.2[M+H, Br].

Int-60A：((1R，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-(((1-(碘甲基)环丙基)甲基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯的合成Int-60A: Synthesis of tert-butyl ((1R,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-(((1-(iodomethyl)cyclopropyl)methyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate

步骤1：(1R，3R)-5-氨基-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-(((1-(羟基甲基)环丙基)甲基)亚氨基)-3，6，6-三甲基-3，6-二氢-2H-1，4-噻嗪1-氧化物(Int-58A)Step 1: (1R,3R)-5-amino-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-(((1-(hydroxymethyl)cyclopropyl)methyl)imino)-3,6,6-trimethyl-3,6-dihydro-2H-1,4-thiazine 1-oxide (Int-58A)

将2-((R，2R)-2-氨基-2-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-N-((1-(羟基甲基)环丙基)甲基)丙基磺酰亚胺基)-2-甲基丙腈(Int-57A，1.8g，3.2mmol)溶解在乙醇(30mL)中并且加入溴化铜(I)(460mg，3.2mmol)。将混合物加热至85℃并且在该温度搅拌90min。之后，将其冷却至室温并且倒入盐水(100mL)和氨水(32％w/w，40mL)的混合物中。将得到的混合物用乙酸乙酯(2x100mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩，得到浅黄色泡沫，为粗产物。将粗产物通过柱色谱(硅胶，40g，用乙酸乙酯/甲醇中2N氨，梯度98∶2至90∶10洗脱)纯化，在真空中干燥(40℃，5毫巴)后，得到标题化合物，为白色泡沫(1.4g，78％)。¹H NMR(CDCl₃，300MHz)：δ0.25-0.49(m，4H)，0.81-0.92(m，6H)，0.93-1.02(m，9H)，1.66(s，3H)，1.72(s，3H)，1.77(s，3H)，2.80(br s，1H)，3.02(d，J＝11.9Hz，1H)，3.18-3.51(m，4H)，4.20(d，J＝14.7Hz，1H)，7.33(d，J＝2.4Hz，1H)。MS(ES+)m/z 563.2&561.2[M+H，Br]。2-((R,2R)-2-amino-2-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-N-((1-(hydroxymethyl)cyclopropyl)methyl)propylsulfonylimino)-2-methylpropionitrile (Int-57A, 1.8 g, 3.2 mmol) was dissolved in ethanol (30 mL) and copper (I) bromide (460 mg, 3.2 mmol) was added. The mixture was heated to 85 ° C and stirred at this temperature for 90 min. Afterwards, it was cooled to room temperature and poured into a mixture of brine (100 mL) and ammonia (32% w / w, 40 mL). The resulting mixture was extracted with ethyl acetate (2x100 mL) and the combined extracts were dried (sodium sulfate) and concentrated in vacuo to give a light yellow foam as a crude product. The crude product was purified by column chromatography (silica gel, 40 g, elution with ethyl acetate/2N ammonia in methanol, gradient 98:2 to 90:10) to give, after drying in vacuo (40° C., 5 mbar), the title compound as a white foam (1.4 g, 78%). ¹ H NMR (CDCl ₃ , 300MHz): δ0.25-0.49 (m, 4H), 0.81-0.92 (m, 6H), 0.93-1.02 (m, 9H), 1.66 (s, 3H), 1.72 (s, 3H), 1.77 (s, 3H), 2.80 (br s, 1H), 3.02 (d, J=11.9Hz, 1H), 3.18-3.51 (m, 4H), 4.20 (d, J=14.7Hz, 1H), 7.33 (d, J=2.4Hz, 1H). MS(ES+)m/z 563.2&561.2[M+H, Br].

步骤2：((1R，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-(((1-(羟基甲基)环丙基)甲基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-59A)Step 2: tert-Butyl ((1R,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-(((1-(hydroxymethyl)cyclopropyl)methyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-59A)

将(1R，3R)-5-氨基-3-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-(((1-(羟基甲基)环丙基)甲基)亚氨基)-3，6，6-三甲基-3，6-二氢-2H-1，4-噻嗪1-氧化物(Int-58A，1.4mg，2.49mmol)溶解在THF(30mL)和水(6mL)中并且加入Boc-酐(816mg，3.74mmol)，碳酸氢钠(293mg，3.49mmol)和4-二甲基氨基吡啶(15.2mg，125μmol)。将混合物在室温搅拌90min。之后，加入氨水(25％w/w，255mg，324μL，3.74mmol)并且将混合物搅拌另外10min。然后将反应混合物用水(80mL)稀释并且用乙酸乙酯(2x80mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，24g，用乙酸乙酯/正庚烷，梯度5∶95至50∶50洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色泡沫(1.52g，92％)。HPLC(方法LCMS_fglm)t_R＝1.70min。¹H NMR(CDCl₃，300MHz)：δ0.21-0.46(m，4H)，0.80-0.92(m，6H)，0.93-1.03(m，9H)，1.54(s，9H)，1.65(s，3H)，1.77(s，3H)，1.85(s，3H)，2.61(d，J＝12.5Hz，1H)，2.94(dd，J＝5.7，6.3Hz，1H)，3.01(d，J＝12.5Hz，1H)，3.35(dd，J＝6.4，11.3Hz，1H)，3.54(d，J＝15.1Hz，1H)，4.45(d，J＝15.1Hz，1H)，7.38(d，J＝2.4Hz，1H)。MS(ES+)m/z 663.2&661.2[M+H，Br]。(1R,3R)-5-amino-3-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-(((1-(hydroxymethyl)cyclopropyl)methyl)imino)-3,6,6-trimethyl-3,6-dihydro-2H-1,4-thiazine 1-oxide (Int-58A, 1.4 mg, 2.49 mmol) was dissolved in THF (30 mL) and water (6 mL) and Boc-anhydride (816 mg, 3.74 mmol), sodium bicarbonate (293 mg, 3.49 mmol) and 4-dimethylaminopyridine (15.2 mg, 125 μmol) were added. The mixture was stirred at room temperature for 90 min. Afterwards, aqueous ammonia (25% w/w, 255 mg, 324 μL, 3.74 mmol) was added and the mixture was stirred for another 10 min. The reaction mixture was then diluted with water (80 mL) and extracted with ethyl acetate (2 x 80 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 24 g, eluted with ethyl acetate/n-heptane, gradient 5:95 to 50:50) and dried in vacuo (40° C., 5 mbar) to give the title compound as a white foam (1.52 g, 92%). HPLC (Method LCMS_fglm) t _R = 1.70 min. ¹ H NMR (CDCl ₃ , 300MHz): δ0.21-0.46 (m, 4H), 0.80-0.92 (m, 6H), 0.93-1.03 (m, 9H), 1.54 (s, 9H), 1.65 (s, 3H), 1.77 (s, 3H), 1.85 (s, 3H), 2.61 (d, J=12.5Hz, 1H), 2.9 4(dd, J=5.7, 6.3Hz, 1H), 3.01 (d, J=12.5Hz, 1H), 3.35 (dd, J=6.4, 11.3Hz, 1H), 3.54 (d, J=15.1Hz, 1H), 4.45 (d, J=15.1Hz, 1H), 7.38 (d, J=2.4Hz, 1H). MS(ES+)m/z 663.2&661.2[M+H, Br].

步骤3：((1R，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-(((1-(碘甲基)环丙基)甲基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-60A)Step 3: tert-Butyl ((1R,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-(((1-(iodomethyl)cyclopropyl)methyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-60A)

将2，3-二氯-5，6-二氰基-1，4-苯醌(626mg，2.76mmol)溶解在二氯甲烷(25mL)中并且加入三苯基膦(723mg，2.76mmol)。将棕色悬浮液冷却至0-5℃(冰浴)并且以一部分加入四正丁基碘化铵(1.02g，2.76mmol)，接着加入((1R，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-(((1-(羟基甲基)环丙基)甲基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-59A，1.52g，2.3mmol)在二氯甲烷(5mL)中的溶液。将反应混合物在0-5℃搅拌15min并且在室温搅拌1h。之后，将其在真空中浓缩并且直接通过柱色谱(硅胶，40g，用乙酸乙酯/正庚烷，梯度5∶95至30∶70洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为无色油状物(1.36g，73％)。HPLC(方法LCMS_梯度)t_R＝4.8min。¹H NMR(CDCl₃，300MHz)：δ0.40-0.50(m，2H)，0.63-0.79(m，2H)，0.81-0.92(m，6H)，0.93-1.02(m，9H)，1.56(s，9H)，1.63(s，3H)，1.77(s，3H)，1.82(s，3H)，2.55(d，J＝12.3Hz，1H)，2.91(d，J＝12.5Hz，1H)，3.15(d，AB，J＝9.7Hz，1H)，3.26(d，AB，J＝9.7Hz，1H)，3.57(d，J＝15.1Hz，1H)，4.41(d，J＝14.9Hz，1H)，7.38(d，J＝2.6Hz，1H)，11.15(s，1H)。MS(ES+)m/z 771.4&773.4[M+H，Br]。2,3-Dichloro-5,6-dicyano-1,4-benzoquinone (626 mg, 2.76 mmol) was dissolved in dichloromethane (25 mL) and triphenylphosphine (723 mg, 2.76 mmol) was added. The brown suspension was cooled to 0-5° C. (ice bath) and tetra-n-butylammonium iodide (1.02 g, 2.76 mmol) was added in one portion, followed by a solution of tert-butyl ((1R,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-(((1-(hydroxymethyl)cyclopropyl)methyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-59A, 1.52 g, 2.3 mmol) in dichloromethane (5 mL). The reaction mixture was stirred at 0-5°C for 15 min and at room temperature for 1 h. Afterwards, it was concentrated in vacuo and purified directly by column chromatography (silica gel, 40 g, eluted with ethyl acetate/n-heptane, gradient 5:95 to 30:70) to give the title compound after drying in vacuo (40°C, 5 mbar) as a colorless oil (1.36 g, 73%). HPLC (method LCMS_Gradient) t _R = 4.8 min. ¹ H NMR (CDCl ₃ , 300MHz): δ0.40-0.50 (m, 2H), 0.63-0.79 (m, 2H), 0.81-0.92 (m, 6H), 0.93-1.02 ( m, 9H), 1.56 (s, 9H), 1.63 (s, 3H), 1.77 (s, 3H), 1.82 (s, 3H), 2.55 (d, J=12.3Hz, 1H ), 2.91 (d, J=12.5Hz, 1H), 3.15 (d, AB, J=9.7Hz, 1H), 3.26 (d, AB, J=9.7Hz, 1H), 3. 57 (d, J=15.1Hz, 1H), 4.41 (d, J=14.9Hz, 1H), 7.38 (d, J=2.6Hz, 1H), 11.15 (s, 1H). MS(ES+)m/z 771.4&773.4[M+H, Br].

Int-61AA和Int-61AB：((4aR，5R，9R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-61AA)和((4aS，5R，9R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-61AB)的合成Int-61AA and Int-61AB: tert-Butyl ((4aR,5R,9R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-61AA and Int-61AB: tert-Butyl ((4aR,5R,9R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate Synthesis of tert-butyl ((4aS,5R,9R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-61AB)

在无水条件下将((1R，5R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-1-(((1-(碘甲基)环丙基)甲基)亚氨基)-2，2，5-三甲基-1-氧代-5，6-二氢-2H-1，4-噻嗪-3-基)氨基甲酸叔丁酯(Int-60A，1.36g，1.76mmol)溶解在THF(20mL)中并且将溶液冷却至＜-70℃(丙酮/干冰浴)。经15min加入LHMDS在THF中的溶液(1.0M，4.58ml，4.58mmol)并且在该温度搅拌45min。然后将得到的澄清，黄色溶液加温至-20℃(冰/乙醇浴)并且在该温度搅拌45min，TLC显示完全转化。通过加入饱和氯化铵水溶液(80mL)将反应终止，并且在相分离之后，将水相用乙酸乙酯(2x80mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，40g，用乙酸乙酯/正庚烷，梯度10∶90至50∶50洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物Int-61AA(首先洗脱，608mg，54％，白色蜡状固体)和Int-61AB(其次洗脱，408mg，36％，无色粘性油状物)，为分离的非对映异构体。Tert-butyl ((1R,5R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-1-(((1-(iodomethyl)cyclopropyl)methyl)imino)-2,2,5-trimethyl-1-oxo-5,6-dihydro-2H-1,4-thiazin-3-yl)carbamate (Int-60A, 1.36 g, 1.76 mmol) was dissolved in THF (20 mL) under anhydrous conditions and the solution was cooled to <-70°C (acetone/dry ice bath). A solution of LHMDS in THF (1.0 M, 4.58 ml, 4.58 mmol) was added over 15 min and stirred at this temperature for 45 min. The resulting clear, yellow solution was then warmed to -20°C (ice/ethanol bath) and stirred at this temperature for 45 min; TLC indicated complete conversion. By adding saturated aqueous ammonium chloride solution (80mL) the reaction is terminated, and after phase separation, aqueous phase is extracted with ethyl acetate (2x80mL).The extract dried (sodium sulfate) that merges is concentrated in vacuo.The crude product is passed through column chromatography (silica gel, 40g, eluted with ethyl acetate/n-heptane, gradient 10: 90 to 50: 50) purifying, after being dried in vacuo (40 ℃, 5 mbar), obtain title compound Int-61AA (first eluting, 608mg, 54%, white waxy solid) and Int-61AB (second eluting, 408mg, 36%, colorless viscous oil), be separated diastereomer.

Int-61AA：HPLC(方法LCMS_梯度)t_R＝4.55min。MS(ES+)m/z 643.3&645.3[M+H，Br]。Int-61AA: HPLC (method LCMS_gradient) _tR = 4.55 min. MS (ES+) m/z 643.3 & 645.3 [M+H, Br].

Int-61AB：HPLC(方法LCMS_梯度)t_R＝4.31min。MS(ES+)m/z 643.4&645.4[M+H，Br]。Int-61AB: HPLC (method LCMS_gradient) _tR = 4.31 min. MS (ES+) m/z 643.4 & 645.4 [M+H, Br].

Int-63AA和Int-63AB：((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-63AA)和((4aS，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-63AB)的合成Int-63AA and Int-63AB: Synthesis of tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-63AA) and tert-butyl ((4aS,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-63AB)

步骤1：((4aR，5R，9R)-5-(6-溴-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-62AA)和((4aS，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-62AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-bromo-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-62AA) and tert-butyl ((4aS,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-62AA)

注释：注意到，在该反应期间，发生在C4a处的差向异构化(AB至AA)。因此，决定继续进行步骤1中的Int-61AA和Int-61AB的混合物并且之后分离非对映异构体。Note: It was noted that during this reaction, epimerization at C4a (AB to AA) occurred. Therefore, it was decided to proceed with the mixture of Int-61AA and Int-61AB in Step 1 and then separate the diastereomers.

将((4aRS，5R，9R)-5-(6-溴-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-61AA&Int-61AB，1016mg，1.58mmol)溶解在THF(24mL)和DMF(6mL)中。在室温加入乙酸(319mg，304μl，5.3mmol)和氟化钾(304mg，5.23mmol)并且将得到的精细悬浮液在该温度搅拌3h。之后，加入碳酸氢钠水溶液(5％m/m，120mL)并且将混合物用叔丁基甲醚(2x150mL)萃取。将合并的萃取物用盐水(2x100mL)洗涤，干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，40g，用乙酸乙酯/正庚烷，梯度20∶80至90∶10洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物Int-62AA(首先洗脱，532mg，64％，白色固体)和Int-62AB(其次洗脱，246mg，29％，白色固体)，为分离的非对映异构体。Tert-butyl ((4aRS,5R,9R)-5-(6-bromo-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-61AA & Int-61AB, 1016 mg, 1.58 mmol) was dissolved in THF (24 mL) and DMF (6 mL). Acetic acid (319 mg, 304 μl, 5.3 mmol) and potassium fluoride (304 mg, 5.23 mmol) were added at room temperature and the resulting fine suspension was stirred at this temperature for 3 h. Afterwards, sodium bicarbonate aqueous solution (5%m/m, 120mL) is added and the mixture is extracted with tert-butyl methyl ether (2x150mL). The combined extracts are washed with saline (2x100mL), dried (sodium sulfate) and concentrated in a vacuum. The crude product is purified by column chromatography (silica gel, 40g, eluted with ethyl acetate/n-heptane, gradient 20: 80 to 90: 10), dried in a vacuum (40°C, 5 mbar) and the title compounds Int-62AA (first eluted, 532mg, 64%, white solid) and Int-62AB (second eluted, 246mg, 29%, white solid) are obtained as separated diastereomers.

Int-62AA：HPLC(方法LCMS_梯度)t_R＝3.1min。¹H NMR(CDCl₃，300MHz)：δ0.37-0.55(m，2H)，0.62-0.75(m，2H)，1.29-1.40(m，1H)，1.50(s，9H)，1.80(2s，6H)，1.89(s，3H)，2.52(d，J＝13.1Hz，1H)，2.76-2.90(m，1H)，4.06(d，J＝12.7Hz，1H)，4.54-4.64(m，1H)，7.36-7.46(m，1H)，7.47-7.55(m，1H)，11.33(s，1H)。MS(ES+)m/z529.3&531.3[M+H，Br]。Int-62AA: HPLC (method LCMS_gradient) t _R =3.1 min. ¹ H NMR (CDCl ₃ , 300 MHz): δ 0.37-0.55 (m, 2H), 0.62-0.75 (m, 2H), 1.29-1.40 (m, 1H), 1.50 (s, 9H), 1.80 (2s, 6H), 1.89 (s, 3H), 2.52 (d, J=13.1 Hz, 1H), 2.76-2.90 (m, 1H), 4.06 (d, J=12.7 Hz, 1H), 4.54-4.64 (m, 1H), 7.36-7.46 (m, 1H), 7.47-7.55 (m, 1H), 11.33 (s, 1H). MS(ES+)m/z529.3&531.3[M+H, Br].

Int-62AB：HPLC(方法LCMS_梯度)t_R＝2.8min。¹H NMR(CDCl₃，300MHz)：δ0.24-0.46(m，3H)，0.60-0.76(m，2H)，1.59(s，9H)，1.80(s，3H)，1.94(s，3H)，2.03(d，J＝0.8Hz，3H)，2.27-2.39(m，1H)，2.79(dd，J＝1.9，13.4Hz，1H)，3.81-3.91(m，2H)，7.38(dd，J＝8.5，10.5Hz，1H)，7.54(dd，J＝3.2，8.5Hz，1H)，11.86(s，1H)。MS(ES+)m/z529.3&531.3[M+H，Br]。Int-62AB: HPLC (method LCMS_gradient) t _R =2.8 min. ¹ H NMR (CDCl ₃ , 300 MHz): δ 0.24-0.46 (m, 3H), 0.60-0.76 (m, 2H), 1.59 (s, 9H), 1.80 (s, 3H), 1.94 (s, 3H), 2.03 (d, J=0.8 Hz, 3H), 2.27-2.39 (m, 1H), 2.79 (dd, J=1.9, 13.4 Hz, 1H), 3.81-3.91 (m, 2H), 7.38 (dd, J=8.5, 10.5 Hz, 1H), 7.54 (dd, J=3.2, 8.5 Hz, 1H), 11.86 (s, 1H). MS(ES+)m/z529.3&531.3[M+H, Br].

步骤2a：((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-63AA)Step 2a: tert-Butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-63AA)

将((4aR，5R，9R)-5-(6-溴-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-62AA，527mg，995μmol)溶解在1，4-二烷(14mL)和水(4.5mL)中并且加入叠氮化钠(893mg，13.7mmol)。接着加入碘化酮(I)(134mg，705μmol)，抗坏血酸钠(67mg，338μmol)和反式-N，N′-二甲基-1，2-环己烷二胺(161mg，181μl，1.13mmol)并且将深绿色混合物在70℃搅拌75min。然后，将反应混合物冷却至室温并且倒入碳酸氢钠水溶液(5％m/m，120mL)中。将其用乙酸乙酯(1x120mL)萃取，并且将合并的萃取物用盐水(100mL)洗涤，经硫酸钠干燥并在真空中浓缩，得到黄色固体，为粗产物。将粗产物通过柱色谱(硅胶，24g，用乙酸乙酯/正庚烷，梯度35∶65至100∶0洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(305mg，66％)。HPLC(方法LCMS_梯度)t_R＝2.5min。¹H NMR(CDCl₃，300MHz)：δ0.31-0.42(m，2H)，0.44-0.50(m，1H)，0.60-0.67(m，2H)，1.17-1.26(m，1H)，1.49(s，9H)，1.79(s，3H)，1.83(s，3H)，1.86(d，J＝1.6Hz，3H)，2.48(dd，J＝2.1，13.0Hz，1H)，2.74-2.86(m，1H)，4.03(dd，J＝1.2，12.9Hz，1H)，4.35(br s，2H)，4.46(dd，J＝3.4，12.3Hz，1H)，6.47(dd，J＝2.5，8.0Hz，1H)，7.26(dd，J＝8.7，11.1Hz，1H)，11.16(s，1H)。MS(ES+)m/z466.3[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-bromo-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-62AA, 527 mg, 995 μmol) was dissolved in 1,4-dioxane (14 mL) and water (4.5 mL) and sodium azide (893 mg, 13.7 mmol) was added. Then iodine ketone (I) (134mg, 705μmol), sodium ascorbate (67mg, 338μmol) and trans-N, N'-dimethyl-1,2-cyclohexanediamine (161mg, 181μl, 1.13mmol) were added and the dark green mixture was stirred at 70°C for 75min. Then, the reaction mixture was cooled to room temperature and poured into an aqueous sodium bicarbonate solution (5%m/m, 120mL). It was extracted with ethyl acetate (1x120mL), and the combined extracts were washed with brine (100mL), dried over sodium sulfate and concentrated in vacuo to give a yellow solid as a crude product. The crude product was purified by column chromatography (silica gel, 24g, eluted with ethyl acetate/n-heptane, gradient 35: 65 to 100: 0) and dried in vacuo (40°C, 5 mbar) to give the title compound as a white solid (305mg, 66%). HPLC (method LCMS_gradient) t _R = 2.5 min. ¹ H NMR (CDCl ₃ , 300 MHz): δ 0.31-0.42 (m, 2H), 0.44-0.50 (m, 1H), 0.60-0.67 (m, 2H), 1.17-1.26 (m, 1H), 1.49 (s, 9H), 1.79 (s, 3H), 1.83 (s, 3H), 1.86 (d, J = 1.6 Hz, 3H), 2.48 (dd, J = 2.1, 13.0 Hz, 1H), 2.74-2.86 (m, 1H), 4.03 (dd, J = 1.2, 12.9 Hz, 1H), 4.35 (br s, 2H), 4.46 (dd, J=3.4, 12.3Hz, 1H), 6.47 (dd, J=2.5, 8.0Hz, 1H), 7.26 (dd, J=8.7, 11.1Hz, 1H), 11.16 (s, 1H). MS(ES+)m/z466.3[M+H].

步骤2b：((4aS，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-63AB)Step 2b: tert-Butyl ((4aS,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-63AB)

将((4aS，5R，9R)-5-(6-溴-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-62AB，240mg，453μmol)溶解在1，4-二烷(15mL)和水(2.5mL)中并且加入叠氮化钠(407mg，6.26mmol)。接着加入碘化酮(I)(61mg，321μmol)，抗坏血酸钠(30mg，154μmol)和反式-N，N′-二甲基-1，2-环己烷二胺(74mg，83μl，0.52mmol)并且将深绿色混合物在70℃搅拌75min。然后，将反应混合物冷却至室温并且倒入碳酸氢钠水溶液(5％m/m，120mL)中，搅拌5min。形成悬浮液，将其过滤，并且用水(4x10mL)洗涤。将沉淀溶解在四氢呋喃(50mL)中，干燥(硫酸钠)并在真空中浓缩，得到黄绿色固体，为粗产物。将粗产物通过柱色谱(硅胶，24g，用二氯甲烷/甲醇中2N氨，梯度99∶1至95∶5洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(123mg，58％)。HPLC(方法LCMS_梯度)t_R＝2.13min。¹H NMR(CDCl₃，300MHz)：δ0.24-0.33(m，1H)，0.35-0.44(m，2H)，0.64-0.84(m，2H)，1.57(s，9H)，1.80(s，3H)，1.93(s，3H)，1.98(m，3H)，2.18-2.30(m，1H)，2.78(dd，J＝1.8，13.5Hz，1H)，3.79-3.92(m，2H)，7.57(s，2H)，6.47(dd，J＝2.5，8.8Hz，1H)，7.24(dd，J＝8.7，10.5Hz，1H)，12.07(s，1H)。MS(ES+)m/z 466.3[M+H]。Tert-butyl ((4aS,5R,9R)-5-(6-bromo-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-62AB, 240 mg, 453 μmol) was dissolved in 1,4-dioxane (15 mL) and water (2.5 mL) and sodium azide (407 mg, 6.26 mmol) was added. Then iodine ketone (I) (61 mg, 321 μmol), sodium ascorbate (30 mg, 154 μmol) and trans-N, N'-dimethyl-1,2-cyclohexanediamine (74 mg, 83 μl, 0.52 mmol) were added and the dark green mixture was stirred at 70 ° C for 75 min. Then, the reaction mixture was cooled to room temperature and poured into an aqueous sodium bicarbonate solution (5% m/m, 120 mL) and stirred for 5 min. A suspension was formed, which was filtered and washed with water (4x10 mL). The precipitate was dissolved in tetrahydrofuran (50 mL), dried (sodium sulfate) and concentrated in vacuo to give a yellow-green solid as a crude product. The crude product was purified by column chromatography (silica gel, 24 g, eluted with 2N ammonia in dichloromethane/methanol, gradient 99: 1 to 95: 5) and dried in vacuo (40 ° C, 5 mbar) to give the title compound as a white solid (123 mg, 58%). HPLC (method LCMS_gradient) _tR = 2.13 min. ¹ H NMR (CDCl ₃ , 300MHz): δ0.24-0.33 (m, 1H), 0.35-0.44 (m, 2H), 0.64-0.84 (m, 2H), 1.57 (s, 9H), 1.80 (s, 3H), 1.93 (s, 3H), 1.98 (m, 3H), 2.18-2.30 (m, 1 H), 2.78 (dd, J=1.8, 13.5Hz, 1H), 3.79-3.92 (m, 2H), 7.57 (s, 2H), 6.47 (dd, J=2.5, 8.8Hz, 1H), 7.24 (dd, J=8.7, 10.5Hz, 1H), 12.07 (s, 1H). MS (ES+) m/z 466.3 [M+H].

实施例Example

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(1AA)N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (1AA)

步骤1：将((4aR，5R，9R)-5-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-18AA)Step 1: Reaction of tert-butyl ((4aR,5R,9R)-5-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-18AA)

将5-氰基-3-甲基吡啶甲酸(200mg，1.23mmol)悬浮在二氯甲烷(4mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(203mg，140μL，1.6mmol)以及一滴二甲基甲酰胺和二氯甲烷(2∶1，v/v)的混合物。将混合物在室温搅拌1.5h。然后，将其在真空中浓缩，将残留物用正庚烷(3mL)处理并再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-氰基-3-甲基吡啶甲酰氯，为红色油状物(220mg，99％)。这之后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-17AA，150mg，341μmol)溶解在二氯甲烷(4mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(75.5mg，100μl，584μmol)，接着加入5-氰基-3-甲基吡啶甲酰氯(见上，80mg，443μmol)在二氯甲烷(2.5mL)中的溶液。将反应混合物在0-5℃搅拌1.5h。然后，将混合物倒到饱和碳酸氢钠水溶液(20mL)上并用二氯甲烷(1x50mL，2x20mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗制物用乙酸乙酯(100mL)研磨，并且经硅胶塞(10g)过滤，用乙酸乙酯(50mL)洗涤。将合并的滤液在真空中浓缩。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度60∶40至80∶20洗脱)纯化，在真空中干燥(50℃，5毫巴)后得到标题化合物，为黄色固体(190mg，95％)。HPLC(方法LCMS_梯度)t_R＝3.1min。¹HNMR(CDCl₃，400MHz)：δ1.47(s，9H)，1.79(s，3H)，1.82-2.02(m，3H)，1.84(s，3H)，1.95(s，3H)，2.34-2.46(m，1H)，2.86(s，3H)，3.36-3.42(m，1H)，3.57-3.66(m，1H)，4.04-4.10(m，1H)，7.57(dd，J＝8.9，10.7Hz，1H)，7.96-7.99(m，1H)，8.41(dd，J＝3.1，9.0Hz，1H)，8.79(d，J＝1.3Hz，1H)，10.41(brs，1H，exch)，11.23(br s，1H，exch)。MS(ES+)m/z 484.3[M+H-CO₂tBu]。5-cyano-3-methylpicolinic acid (200mg, 1.23mmol) is suspended in dichloromethane (4mL), the suspension is cooled to 0-5 ℃ (ice bath) and add oxalyl chloride (203mg, 140 μ L, 1.6mmol) and a mixture of dimethylformamide and dichloromethane (2: 1, v/v).The mixture is stirred at room temperature for 1.5h.Then, it is concentrated in a vacuum, residue is handled and concentrated again with n-heptane (3mL) and dried in a vacuum (40 ℃, 5 mbar), obtain 5-cyano-3-methylpicolinic acid chloride, be red oily matter (220mg, 99%). After this, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-17AA, 150 mg, 341 μmol) was dissolved in dichloromethane (4 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (75.5 mg, 100 μl, 584 μmol) was added, followed by a solution of 5-cyano-3-methylpicolinyl chloride (see above, 80 mg, 443 μmol) in dichloromethane (2.5 mL). The reaction mixture was stirred at 0-5° C. for 1.5 h. The mixture was then poured onto a saturated aqueous sodium bicarbonate solution (20 mL) and extracted with dichloromethane (1 x 50 mL, 2 x 20 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The crude material was triturated with ethyl acetate (100 mL) and filtered through a silica gel plug (10 g), washed with ethyl acetate (50 mL). The combined filtrate was concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 60: 40 to 80: 20) and dried in vacuo (50 ° C, 5 mbar) to give the title compound as a yellow solid (190 mg, 95%). HPLC (method LCMS_gradient) t _R = 3.1 min. ¹ HNMR (CDCl ₃ , 400MHz): δ1.47 (s, 9H), 1.79 (s, 3H), 1.82-2.02 (m, 3H), 1.84 (s, 3H), 1.95 ( s, 3H), 2.34-2.46 (m, 1H), 2.86 (s, 3H), 3.36-3.42 (m, 1H), 3.57-3.66 (m, 1H) , 4.04-4.10 (m, 1H), 7.57 (dd, J=8.9, 10.7Hz, 1H), 7.96-7.99 (m, 1H), 8.41 (d d, J=3.1, 9.0Hz, 1H), 8.79 (d, J=1.3Hz, 1H), 10.41 (brs, 1H, exch), 11.23 (br s, 1H, exch). MS(ES+)m/z 484.3[M+H-CO ₂ tBu].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(1AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (1AA)

将((4aR，5R，9R)-5-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-18AA，180mg，308μmol)溶解在二氯甲烷(10mL)中并且加入三氟乙酸(2.66g，1.8nL，23.4mmol)。将溶液在室温搅拌0.5h。之后，将其在真空中浓缩(25℃，5毫巴)。将残留物棕色粘性油状物溶解在二氯甲烷(40mL)中，并且加入饱和碳酸氢钠水溶液(20mL)。搅拌5min后，分离相并且将水相用二氯甲烷(2x15mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物溶解在二氯甲烷(1mL)中，加入MTBE(5mL)并且再次在真空中浓缩，在真空中干燥(50℃，5毫巴)后得到灰白色固体，为粗产物，将其通过手性制备型HPLC(Chiralpak AD-H，250*4.6mm*5μm，等度，(正庚烷+0.2％三乙胺)/(乙醇+0.1％三乙胺)60/40，流速1.0mL/min)纯化，得到标题化合物，为白色粉末(79mg，53％)。为了转移的目的，将材料溶解在二氯甲烷(2mL)和MTBE(8mL)中并浓缩并在真空中在50℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.4min。¹H NMR(CDCl₃，400MHz)：δ1.65-1.90(m，3H)，1.72(s，3H)，1.82(s，3H)，1.85(s，3H)，2.25-2.42(m，1H)，2.87(s，3H)，3.36(br d，J＝12.4Hz，1H)，3.52-3.68(m，1H)，3.80(br d，J＝12.1Hz，1H)，4.36(br s，2H，exch)，7.47(dd，J＝9.8，9.8Hz，1H)，7.97(s，1H)，8.31(dd，J＝2.4，8.6Hz，1H)，8.79(s，1H)，10.38(br s，1H，exch)。MS(ES+)m/z484.3[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-18AA, 180 mg, 308 μmol) was dissolved in dichloromethane (10 mL) and trifluoroacetic acid (2.66 g, 1.8 nL, 23.4 mmol) was added. The solution was stirred at room temperature for 0.5 h. Afterwards, it was concentrated in vacuo (25 ° C, 5 mbar). The residue, a brown viscous oil, was dissolved in dichloromethane (40 mL) and saturated aqueous sodium bicarbonate (20 mL) was added. The product of 4-nitro- 4- pyridine-2-yl pyridine-3-yl pyridine-4-yl pyridine- ₃ ... ¹ H NMR (CDCl ₃ , 400MHz): δ1.65-1.90 (m, 3H), 1.72 (s, 3H), 1.82 (s, 3H), 1.85 (s, 3H), 2.25-2.42 (m, 1H), 2.87 (s, 3H), 3.36 (br d, J＝12.4Hz, 1H), 3.52-3.68(m, 1H), 3.80(br d, J＝12.1Hz, 1H), 4.36(br s, 2H, exch), 7.47 (dd, J=9.8, 9.8Hz, 1H), 7.97 (s, 1H), 8.31 (dd, J=2.4, 8.6Hz, 1H), 8.79 (s, 1H), 10.38 (br s, 1H, exch). MS(ES+)m/z484.3[M+H].

N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(1AB)N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (1AB)

步骤1：((4aS，5R，9R)-5-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-18AB)Step 1: tert-Butyl ((4aS,5R,9R)-5-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-18AB)

将5-氰基-3-甲基吡啶甲酸(85mg，524μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入1-氯-N，N，2-三甲基丙烯基胺(140mg，140μl，1.05mmol)。在0℃搅拌30min后，在-5℃经5min加入((4aS，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-17AB，150mg，341μmol)和N，N-二异丙基乙胺(148mg，200μL，1.15mmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌15min并且加温至室温。然后，将混合物倒到饱和碳酸氢钠水溶液(20mL)上并且用二氯甲烷(1x50mL，2x20mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物用乙酸乙酯(100mL)研磨，并且经硅胶塞(10g)过滤，将其用乙酸乙酯(50mL)洗涤。将合并的滤液在真空中浓缩。将粗产物通过柱色谱(硅胶，50g，用二氯甲烷/甲醇，梯度99∶1至95∶5洗脱)纯化，在真空中干燥(50℃，5毫巴)后得到黄色粘性固体。将该材料悬浮在MTBE(3mL)和正庚烷(2mL)的混合物中，过滤沉淀，用MTBE/正庚烷(1∶3v/v，4mL)的混合物洗涤并在真空中干燥(50℃，5毫巴)，得到标题化合物，为灰白色固体(50mg，25％)。HPLC(方法LCMS_梯度)t_R＝3.0min。¹H NMR(CDCl₃，400MHz)：δ1.62-1.71(m，2H)，1.65(s，9H)，1.74-1.84(m，2H)，1.82(s，3H)，1.91(s，3H)，2.04(s，3H)，2.88(s，3H)，3.36-3.46(m，1H)，3.62-3.71(m，2H)，7.59(dd，J＝9.0，10.0Hz，1H)，7.98-8.00(m，1H)，8.47(dd，J＝3.2，8.9Hz，1H)，8.71(d，J＝1.3Hz，1H)，10.76(brs，1H，exch)，12.40(br s，1H，exch)。MS(ES+)m/z 584.4[M+H]。5-Cyano-3-methylpicolinic acid (85 mg, 524 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and 1-chloro-N,N,2-trimethylpropenylamine (140 mg, 140 μl, 1.05 mmol) was added. After stirring at 0° C. for 30 min, a solution of tert-butyl ((4aS,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-17AB, 150 mg, 341 μmol) and N,N-diisopropylethylamine (148 mg, 200 μL, 1.15 mmol) in dichloromethane (3 mL) was added at −5° C. over 5 min. The reaction mixture was stirred at 0-5° C. for 15 min and warmed to room temperature. The mixture was then poured onto saturated aqueous sodium bicarbonate (20 mL) and extracted with dichloromethane (1×50 mL, 2×20 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The crude product is ground with ethyl acetate (100mL) and filtered through a silica gel plug (10g), which is washed with ethyl acetate (50mL). The combined filtrate is concentrated in vacuo. The crude product is purified by column chromatography (silica gel, 50g, eluted with dichloromethane/methanol, gradient 99: 1 to 95: 5), dried in vacuo (50°C, 5 mbar) to obtain a yellow sticky solid. This material is suspended in a mixture of MTBE (3mL) and normal heptane (2mL), filtered and precipitated, washed with a mixture of MTBE/ normal heptane (1: 3v/v, 4mL) and dried in vacuo (50°C, 5 mbar) to obtain the title compound as a pale white solid (50mg, 25%). HPLC (method LCMS-gradient) _tR = 3.0min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.62-1.71 (m, 2H), 1.65 (s, 9H), 1.74-1.84 (m, 2H), 1.82 (s, 3H), 1.91(s, 3H), 2.04(s, 3H), 2.88(s, 3H), 3.36-3.46(m, 1H), 3.62-3. 71 (m, 2H), 7.59 (dd, J=9.0, 10.0Hz, 1H), 7.98-8.00 (m, 1H), 8.47 (dd, J=3.2, 8.9Hz, 1H), 8.71 (d, J=1.3Hz, 1H), 10.76 (brs, 1H, exch), 12.40 (br s, 1H, exch). MS(ES+)m/z 584.4[M+H].

步骤2：N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(1AB)Step 2: N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (1AB)

将((4aS，5R，9R)-5-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-18AB，50mg，85.7μmol)溶解在二氯甲烷(3mL)中并且加入三氟乙酸(740mg，500μL，6.49mmol)。将溶液在室温搅拌2h。之后，将其在真空中浓缩(25℃，5毫巴)。将残留物棕色粘性油状物溶解在二氯甲烷(30mL)中，加入饱和碳酸氢钠水溶液(10mL)。在搅拌5min后，分离相并且将水相用二氯甲烷(2x15mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物溶解在二氯甲烷(1mL)中，加入MTBE(3mL)并再次在真空中浓缩，在真空中干燥(50℃，5毫巴)后得到灰白色固体，为粗产物，将其通过手性制备型HPLC(Chiralpak AD-H，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.1％三乙胺)50/50，流速1.0mL/min)纯化，得到标题化合物，为白色粉末(16mg，39％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和MTBE(3mL)中并浓缩并在真空中在50℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.3min。¹H NMR(CDCl₃，400MHz)：δ1.65-2.06(m，4H)，1.70(s，3H)，1.86(s，3H)，1.92(s，3H)，2.87(s，3H)，3.45-3.54(m，1H)，3.56-3.64(m，1H)，3.66-3.74(m，1H)，7.55(dd，J＝9.1，10.0Hz，1H)，7.98(s，1H)，8.42(dd，J＝3.0，8.9Hz，1H)，8.80(s，1H)，10.59(br s，1H，exch)。MS(ES+)m/z484.3[M+H]。Tert-butyl ((4aS,5R,9R)-5-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-18AB, 50 mg, 85.7 μmol) was dissolved in dichloromethane (3 mL) and trifluoroacetic acid (740 mg, 500 μL, 6.49 mmol) was added. The solution was stirred at room temperature for 2 h. Afterwards, it was concentrated in vacuo (25 ° C, 5 mbar). The residual brown viscous oil was dissolved in dichloromethane (30 mL) and saturated aqueous sodium bicarbonate solution (10 mL) was added. After stirring for 5 min, the phases were separated and the aqueous phase was extracted with dichloromethane (2x15 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was dissolved in dichloromethane (1 mL), MTBE (3 mL) was added and concentrated again in vacuo. After drying in vacuo (50° C., 5 mbar), an off-white solid was obtained as a crude product, which was purified by chiral preparative HPLC (Chiralpak AD-H, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.1% triethylamine) 50/50, flow rate 1.0 mL/min) to give the title compound as a white powder (16 mg, 39%). For transfer purposes, the material was dissolved in dichloromethane (1 mL) and MTBE (3 mL) and concentrated and dried in vacuo at 50° C./5 mbar. HPLC (method LCMS_gradient) t _R =1.3 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.65-2.06 (m, 4H), 1.70 (s, 3H), 1.86 (s, 3H), 1.92 (s, 3H), 2.87 (s, 3H), 3.45-3.54 (m, 1H), 3.56-3.64 (m, 1H), 3.66-3.74 (m, 1H), 7.55 (dd, J=9.1, 10.0Hz, 1H), 7.98 (s, 1H), 8.42 (dd, J=3.0, 8.9Hz, 1H), 8.80 (s, 1H), 10.59 (br s, 1H, exch). MS(ES+)m/z484.3[M+H].

N-(6-((4aR，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(1BA)N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (1BA)

步骤1：((4aR，5R，9S)-5-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-18BA)Step 1: tert-Butyl ((4aR,5R,9S)-5-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-18BA)

将5-氰基-3-甲基吡啶甲酸(100mg，617μmol)悬浮在二氯甲烷(2mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(102mg，70μL，0.8mmol)以及一滴二甲基甲酰胺和二氯甲烷(1∶3，v/v)的混合物。将混合物在室温搅拌1h。然后将其在真空中浓缩，将残留物用正庚烷(2mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-氰基-3-甲基吡啶甲酰氯，为红色油状物(110mg)。其后，将((4aR，5R，9S)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-17AB，150mg，341μmol)溶解在二氯甲烷(4mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(75.5mg，100μl，584μmol)，接着加入5-氰基-3-甲基吡啶甲酰氯(见上，80mg，443μmol)在二氯甲烷(2.5mL)中的溶液。将反应混合物在0-5℃搅拌1.5h。然后，将混合物倒到饱和碳酸氢钠水溶液(20mL)上并用二氯甲烷(1x50mL，2x20mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度60∶40至80∶20洗脱)纯化，在真空中干燥(50℃，5毫巴)后得到标题化合物，为黄色固体(135mg，68％产率)。HPLC(方法LCMS_梯度)t_R＝3.0min。¹H NMR(CDCl₃，400MHz)：δ1.34(s，3H)，1.56(s，9H)，1.79(s，3H)，1.82(s，3H)，1.86-1.97(m，2H)，2.16-2.29(m，1H)，2.53-2.62(m，1H)，2.85(s，3H)，3.52(ddd，J＝5.6，7.9，13.6Hz，1H)，3.67(ddd，J＝4.6，4.6，13.4Hz，1H)，3.98(dd，J＝4.7，10.6Hz，1H)，7.48(dd，J＝9.0，10.6Hz，1H)，7.96(dd，J＝0.8，1.9Hz，1H)，8.36(dd，J＝3.0，8.9Hz，1H)，8.74-8.77(m，1H)，10.31(br s，1H，exch)，10.78(br s，1H，exch)。MS(ES+)m/z584.4[M+H]。5-cyano-3-methylpicolinic acid (100mg, 617μmol) is suspended in dichloromethane (2mL), the suspension is cooled to 0-5 ℃ (ice bath) and oxalyl chloride (102mg, 70μL, 0.8mmol) and a drop of dimethylformamide and dichloromethane (1: 3, v/v) are added. The mixture is stirred at room temperature for 1h. The mixture is then concentrated in vacuo, the residue is processed with n-heptane (2mL) and concentrated again and dried in vacuo (40 ℃, 5 mbar) to obtain 5-cyano-3-methylpicolinic acid chloride as a red oil (110mg). Subsequently, tert-butyl ((4aR,5R,9S)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-17AB, 150 mg, 341 μmol) was dissolved in dichloromethane (4 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (75.5 mg, 100 μl, 584 μmol) was added, followed by a solution of 5-cyano-3-methylpicolinoyl chloride (see above, 80 mg, 443 μmol) in dichloromethane (2.5 mL). The reaction mixture was stirred at 0-5° C. for 1.5 h. The mixture was then poured onto saturated aqueous sodium bicarbonate solution (20 mL) and extracted with dichloromethane (1 x 50 mL, 2 x 20 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 60:40 to 80:20) and dried in vacuo (50° C., 5 mbar) to give the title compound as a yellow solid (135 mg, 68% yield). HPLC (Method LCMS_Gradient) t _R = 3.0 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.34 (s, 3H), 1.56 (s, 9H), 1.79 (s, 3H), 1.82 (s, 3H), 1.86-1.97 (m, 2H), 2.1 6-2.29 (m, 1H), 2.53-2.62 (m, 1H), 2.85 (s, 3H), 3.52 (ddd, J=5.6, 7.9, 13.6Hz, 1H), 3.67 (ddd, J=4.6, 4.6, 13.4Hz, 1H), 3.98 (dd, J=4.7, 10.6Hz, 1H), 7.48 (dd, J=9.0, 10.6Hz, 1H ), 7.96 (dd, J=0.8, 1.9Hz, 1H), 8.36 (dd, J=3.0, 8.9Hz, 1H), 8.74-8.77 (m, 1H), 10.31 (br s, 1H, exch), 10.78 (br s, 1H, exch). MS(ES+)m/z584.4[M+H].

步骤2：N-(6-((4aR，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(1BA)Step 2: N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (1BA)

将((4aR，5R，9S)-5-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-18BA，130mg，223μmol)溶解在二氯甲烷(7mL)中并且加入三氟乙酸(1.92g，1.3mL，16.9mmol)。将溶液在室温搅拌0.5h。之后，将其在真空中浓缩(25℃，5毫巴)。将残留物棕色粘性油状物溶解在二氯甲烷(40mL)中，并且加入饱和碳酸氢钠水溶液(20mL)。搅拌5min后，分离相并且将水相用二氯甲烷(2x15mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物溶解在二氯甲烷(1mL)中，加入MTBE(5mL)并再次在真空中浓缩，在真空中干燥(50℃，5毫巴)后得到灰白色固体，为粗产物。将粗产物粗制通过手性制备型HPLC(Reprosil手性-NR，250*4.6mm*5μm，等度，(正庚烷/(乙醇+0.1％乙酸铵)60/40，流速1.0mL/min)纯化，得到标题化合物，为灰白色粉末(89mg，83％)。为了转移的目的，将材料溶解在二氯甲烷(2mL)和MTBE(8mL)中并浓缩并在真空中在50℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.2min。¹H NMR(CDCl₃，400MHz)：δ1.55(s，3H)，1.88-1.99(m，2H)，1.99(s，3H)，2.01(s，3H)，2.07(s，3H)，2.08-2.20(m，1H)，2.61-2.70(m，1H)，2.73(s，3H)，3.42-3.54(m，2H)，3.66-3.74(m，1H)，7.51(dd，J＝9.0，10.1Hz，1H)，7.70-7.74(m，1H)，8.08(dd，J＝3.0，8.6Hz，1H)，8.77(d，J＝1.3Hz，1H)，10.30(br s，1H，exch)。MS(ES+)m/z 484.3[M+H]。Tert-butyl ((4aR,5R,9S)-5-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-18BA, 130 mg, 223 μmol) was dissolved in dichloromethane (7 mL) and trifluoroacetic acid (1.92 g, 1.3 mL, 16.9 mmol) was added. The solution was stirred at room temperature for 0.5 h. Afterwards, it was concentrated in vacuo (25 ° C, 5 mbar). The residue, a brown viscous oil, was dissolved in dichloromethane (40 mL) and saturated aqueous sodium bicarbonate (20 mL) was added. After stirring for 5min, the phases were separated and the aqueous phase was extracted with dichloromethane (2x15mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was dissolved in dichloromethane (1mL), MTBE (5mL) was added and concentrated in vacuo again, and after drying in vacuo (50°C, 5 mbar), an off-white solid was obtained, which was a crude product. The crude product was purified by chiral preparative HPLC (Reprosil chiral-NR, 250*4.6mm*5μm, isocratic, (n-heptane/(ethanol+0.1% ammonium acetate) 60/40, flow rate 1.0mL/min) to obtain the title compound, which was an off-white powder (89mg, 83%). For the purpose of transfer, the material was dissolved in dichloromethane (2mL) and MTBE (8mL) and concentrated and dried in vacuo at 50°C/5 mbar. HPLC (method LCMS_ gradient) t _R =1.2min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.55 (s, 3H), 1.88-1.99 (m, 2H), 1.99 (s, 3H), 2.01 (s, 3H), 2.07(s, 3H), 2.08-2.20(m, 1H), 2.61-2.70(m, 1H), 2.73(s, 3H), 3.42- 3.54 (m, 2H), 3.66-3.74 (m, 1H), 7.51 (dd, J=9.0, 10.1Hz, 1H), 7.70-7.74 (m, 1H), 8.08 (dd, J=3.0, 8.6Hz, 1H), 8.77 (d, J=1.3Hz, 1H), 10.30 (br s, 1H, exch). MS(ES+)m/z 484.3[M+H].

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺(2AA)N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide (2AA)

步骤1：((4aR，5R，9R)-5-(3-氟-6-(5-甲氧基吡嗪-2-甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-22AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(3-fluoro-6-(5-methoxypyrazine-2-carboxamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-22AA)

将5-甲氧基吡嗪-2-甲酸(100mg，649μmol)悬浮在二氯甲烷(2mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(218mg，150μl，1.71mmol)以及一滴二甲基甲酰胺和二氯甲烷(1∶3，v/v)的混合物。将混合物在室温搅拌1.5h。然后将其在真空中在40℃浓缩，将残留物用正庚烷(2mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-甲氧基吡嗪-2-甲酰氯，为红色油状物(110mg)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-17AA，100mg，228μmol)溶解在二氯甲烷(4mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(75.5mg，100μl，584μmol)，接着加入5-甲氧基吡嗪-2-甲酰氯(见上，50mg，290μmol)在二氯甲烷(1mL)中的溶液。将反应混合物在0-5℃搅拌1.5h。然后，加入乙醇(100μl)，将混合物在室温搅拌15min，倒到饱和碳酸氢钠水溶液(15mL)上并用二氯甲烷(1x30mL，2x20mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度60∶40至80∶20洗脱)纯化，在真空中干燥(50℃，5毫巴)后得到标题化合物，为黄色固体(120mg，92％产率)。HPLC(方法LCMS_梯度)t_R＝3.0min。¹H NMR(CDCl₃，400MHz)：δ1.48(s，9H)，1.79(s，3H)，1.82-2.04(m，3H)，1.85(s，3H)，1.95(s，3H)，2.40(dddd，J＝3.8，12.6，12.6，12.6Hz，1H)，3.36-3.44(m，1H)，3.58-3.66(m，1H)，4.05-4.11(m，1H)，4.09(s，3H)，7.57(dd，J＝9.0，10.9Hz，1H)，8.23(d，J＝1.3Hz，1H)，8.44(dd，J＝3.0，8.9Hz，1H)，9.04(d，J＝1.3Hz，1H)，9.94(br s，1H)，11.24(br s，1H，exch)。MS(ES+)m/z 576.4[M+H]。5-Methoxypyrazine-2-carboxylic acid (100 mg, 649 μmol) was suspended in dichloromethane (2 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (218 mg, 150 μl, 1.71 mmol) and a drop of dimethylformamide and dichloromethane (1: 3, v/v) were added. The mixture was stirred at room temperature for 1.5 h. The product was then concentrated at 40° C. in a vacuum, the residue was treated with n-heptane (2 mL) and concentrated again and dried in a vacuum (40° C., 5 mbar) to give 5-methoxypyrazine-2-carboxylic acid chloride as a red oil (110 mg). Subsequently, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-17AA, 100 mg, 228 μmol) was dissolved in dichloromethane (4 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (75.5 mg, 100 μl, 584 μmol) was added, followed by a solution of 5-methoxypyrazine-2-carbonyl chloride (see above, 50 mg, 290 μmol) in dichloromethane (1 mL). The reaction mixture was stirred at 0-5° C. for 1.5 h. Then, ethanol (100 μl) was added, the mixture was stirred at room temperature for 15 min, poured onto saturated aqueous sodium bicarbonate solution (15 mL) and extracted with dichloromethane (1 x 30 mL, 2 x 20 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 60: 40 to 80: 20) and dried in vacuo (50° C., 5 mbar) to give the title compound as a yellow solid (120 mg, 92% yield). HPLC (Method LCMS_Gradient) t _R = 3.0 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.48 (s, 9H), 1.79 (s, 3H), 1.82-2.04 (m, 3H), 1.85 (s, 3H), 1.95 (s , 3H), 2.40 (dddd, J=3.8, 12.6, 12.6, 12.6Hz, 1H), 3.36-3.44 (m, 1H), 3.58-3. 66 (m, 1H), 4.05-4.11 (m, 1H), 4.09 (s, 3H), 7.57 (dd, J=9.0, 10.9Hz, 1H), 8.23 (d, J=1.3Hz, 1H), 8.44 (dd, J=3.0, 8.9Hz, 1H), 9.04 (d, J=1.3Hz, 1H), 9.94 (br s, 1H), 11.24 (br s, 1H, exch). MS(ES+)m/z 576.4[M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺(2AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide (2AA)

将((4aR，5R，9R)-5-(3-氟-6-(5-甲氧基吡嗪-2-甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-22AA，120mg，208μmol)溶解在二氯甲烷(6mL)中并且加入三氟乙酸(1.78g，1.2mL，15.6mmol)。将溶液在室温搅拌0.5h。之后，将其在真空中浓缩(25℃，5毫巴)。将残留物棕色粘性油状物溶解在二氯甲烷(40mL)中，并且加入饱和碳酸氢钠水溶液(15mL)。搅拌5min后，分离相并且将水相用二氯甲烷(2x15mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物溶解在二氯甲烷(1mL)中，加入MTBE(5mL)并且再次在真空中浓缩，在真空中干燥(50℃，5毫巴)后得到灰白色固体，为粗产物。将粗产物通过手性制备型HPLC(ChiralpakAD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.1％乙酸铵)80/20，流速1.0mL/min)纯化，得到标题化合物，为白色粉末(48mg，48％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和MTBE(4mL)中并浓缩并在真空中在50℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.4min。¹HNMR(CDCl₃，400MHz)：δ1.72-1.94(m，3H)，1.84(s，3H)，1.92(2s，6H)，2.27-2.41(m，1H)，3.33-3.41(m，1H)，3.53-3.63(m，1H)，3.80(dd，J＝3.2，12.6Hz，1H)，4.09(s，3H)，7.53(dd，J＝8.9，10.9Hz，1H)，8.22(d，J＝1.1Hz，1H)，8.41(dd，J＝3.0，8.9Hz，1H)，9.03(d，J＝1.1Hz，1H)，9.96(br s，1H)。MS(ES+)m/z 476.3[M+H]。Tert-butyl ((4aR,5R,9R)-5-(3-fluoro-6-(5-methoxypyrazine-2-carboxamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-22AA, 120 mg, 208 μmol) was dissolved in dichloromethane (6 mL) and trifluoroacetic acid (1.78 g, 1.2 mL, 15.6 mmol) was added. The solution was stirred at room temperature for 0.5 h. Afterwards, it was concentrated in vacuo (25° C., 5 mbar). The residue, a brown viscous oil, was dissolved in dichloromethane (40 mL) and saturated aqueous sodium bicarbonate (15 mL) was added. In 40mL 40mL HCl (50mL) of 4-nitropropane-2-ol (40mg, 48%), 4-nitropropane-2-ol (40mg, 48%) was added to the 4-nitropropane-2-ol (40mL) solution of 4-nitropropane-2-ol (40mL) and 4-nitropropane-2-ol (40mL).Then, the mixture was stirred for 5 min, the phases were separated and the aqueous phase was extracted with dichloromethane (2x15mL). The combined extracts were dried (sodium sulfate) and concentrated in a vacuum. The residue was dissolved in dichloromethane (1mL), MTBE (5mL) was added and concentrated in a vacuum again, and after drying in a vacuum (50°C, 5 mbar), an off-white solid was obtained, which was a crude product. The crude product was purified by chiral preparative HPLC (Chiralpak AD, 250*4.6mm*5 μm, isocratic, n-heptane/(ethanol+0.1% ammonium acetate) 80/20, flow velocity 1.0mL/min) to obtain the title compound, which was a white powder (48mg, 48%). For the purpose of transfer, the material was dissolved in dichloromethane (1mL) and MTBE (4mL) and concentrated and dried in a vacuum at 50°C/5 mbar. HPLC (method LCMS_ gradient) t _R =1.4min. ^1HNMR (CDCl ₃ , 400MHz): δ1.72-1.94 (m, 3H), 1.84 (s, 3H), 1.92 (2s, 6H), 2.27-2.41 (m, 1H), 3.33-3.41 (m, 1H), 3.53-3.63 (m, 1H), 3.80 (dd, J=3.2, 12 .6Hz, 1H), 4.09 (s, 3H), 7.53 (dd, J=8.9, 10.9Hz, 1H), 8.22 (d, J=1.1Hz, 1H), 8.41 (dd, J=3.0, 8.9Hz, 1H), 9.03 (d, J=1.1Hz, 1H), 9.96 (br s, 1H). MS (ES+) m/z 476.3 [M+H].

N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺(2AB)N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide (2AB)

步骤1：((4aS，5R，9R)-5-(3-氟-6-(5-甲氧基吡嗪-2-甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-22AB)Step 1: tert-Butyl ((4aS,5R,9R)-5-(3-fluoro-6-(5-methoxypyrazine-2-carboxamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-22AB)

将5-甲氧基吡嗪-2-甲酸(200mg，1.3mmol)悬浮在二氯甲烷(5mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(435mg，300μl，3.43mmol)以及一滴二甲基甲酰胺和甲苯(1∶3，v/v)的混合物。将混合物在室温搅拌2h。然后，将其在真空中在40℃浓缩，将残留物用正庚烷(2mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-甲氧基吡嗪-2-甲酰氯，为红色油状物(229mg)。其后，将((4aS，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-17AB，70mg，159μmol)溶解在二氯甲烷(4mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(53mg，70μl，409μmol)，接着加入5-甲氧基吡嗪-2-甲酰氯(见上，100mg，579μmol)在二氯甲烷(1.3mL)中的溶液。将反应混合物在室温搅拌4h。然后，加入乙醇(200μl)，将混合物在室温搅拌30min，倒到饱和碳酸氢钠水溶液(15mL)上并用二氯甲烷(1x30mL，2x20mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度60∶40至80∶20洗脱)纯化，在真空中干燥(50℃，5毫巴)后得到标题化合物，为白色固体(90mg，98％产率)。HPLC(方法LCMS_梯度)t_R＝2.9min。¹H NMR(CDCl₃，400MHz)：δ1.62-1.88(m，4H)，1.66(s，9H)，1.82(s，3H)，1.91(s，3H)，2.04(s，3H)，3.36-3.45(m，1H)，3.62-3.71(m，2H)，4.09(s，3H)，7.59(dd，J＝9.0，10.1Hz，1H)，8.14(d，J＝1.3Hz，1H)，8.48(dd，J＝3.0，8.9Hz，1H)，9.06(d，J＝1.3Hz，1H)，10.36(br s，1H)，12.49(br s，1H)。MS(ES+)m/z 576.3[M+H]。5-Methoxypyrazine-2-carboxylic acid (200 mg, 1.3 mmol) is suspended in dichloromethane (5 mL), the suspension is cooled to 0-5 ° C (ice bath) and oxalyl chloride (435 mg, 300 μ l, 3.43 mmol) and a drop of dimethylformamide and toluene (1: 3, v/v) are added. The mixture is stirred at room temperature for 2 h. Then, it is concentrated at 40 ° C in a vacuum, the residue is treated with n-heptane (2 mL) and concentrated again and dried in a vacuum (40 ° C, 5 mbar) to obtain 5-methoxypyrazine-2-carbonyl chloride as a red oil (229 mg). Subsequently, tert-butyl ((4aS,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-17AB, 70 mg, 159 μmol) was dissolved in dichloromethane (4 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (53 mg, 70 μl, 409 μmol) was added, followed by a solution of 5-methoxypyrazine-2-carbonyl chloride (see above, 100 mg, 579 μmol) in dichloromethane (1.3 mL). The reaction mixture was stirred at room temperature for 4 h. The product was concentrated under reduced pressure and dried (50 ° C, 5 mbar) for 10 min.Then, ethanol (200 μ l) was added and the mixture was stirred at room temperature for 30 min, poured onto a saturated aqueous sodium bicarbonate solution (15 mL) and extracted with dichloromethane (1x30 mL, 2x20 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 60: 40 to 80: 20) and the title compound was obtained after drying in vacuo (50 ° C, 5 mbar) as a white solid (90 mg, 98% yield). HPLC (method LCMS-gradient) t _R = 2.9 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.62-1.88 (m, 4H), 1.66 (s, 9H), 1.82 (s, 3H), 1.91 (s, 3H), 2.04 (s, 3H), 3.36-3.45 (m, 1H), 3.62-3.71 (m, 2H), 4. 09 (s, 3H), 7.59 (dd, J=9.0, 10.1Hz, 1H), 8.14 (d, J=1.3Hz, 1H), 8.48 (dd, J=3.0, 8.9Hz, 1H), 9.06 (d, J=1.3Hz, 1H), 10.36 (br s, 1H), 12.49 (br s, 1H). MS (ES+) m/z 576.3 [M+H].

步骤2：N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺(2AB)Step 2: N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide (2AB)

将((4aS，5R，9R)-5-(3-氟-6-(5-甲氧基吡嗪-2-甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-22AB，85mg，148μmol)溶解在二氯甲烷(4mL)中并且加入三氟乙酸(740mg，500μL，6.5mmol)。将溶液在室温搅拌1h。之后，将其在真空中浓缩(25℃，5毫巴)。将残留物棕色粘性油状物溶解在二氯甲烷(40mL)中，并且加入饱和碳酸氢钠水溶液(15mL)。搅拌5min后，分离相并且将水相用二氯甲烷(2x15mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物溶解在二氯甲烷(1mL)中，加入MTBE(5mL)并再次在真空中浓缩，在真空中干燥(50℃，5毫巴)后得到灰白色固体，为粗产物。将粗产物通过手性制备型HPLC(ChiralpakIE，250*4.6mm*5μm，等度，(异丙醇+0.1％三乙胺)/二氯甲烷90/10，流速0.7mL/min)纯化，得到标题化合物，为白色粉末(29mg，41％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和MTBE(4mL)中并浓缩并在真空中在50℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.3min。¹HNMR(CDCl₃，400MHz)：δ1.64-2.01(m，4H)，1.71(s，3H)，1.86(s，3H)，1.93(s，3H)，3.41-3.53(m，1H)，3.54-3.63(m，1H)，3.64-3.74(m，1H)，4.09(s，3H)，7.55(dd，J＝9.4，9.9Hz，1H)，8.23(s，1H)，8.44(dd，J＝3.0，8.9Hz，1H)，9.03(s，1H)，10.19(br s，1H)。MS(ES+)m/z476.2[M+H]。Tert-butyl ((4aS,5R,9R)-5-(3-fluoro-6-(5-methoxypyrazine-2-carboxamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-22AB, 85 mg, 148 μmol) was dissolved in dichloromethane (4 mL) and trifluoroacetic acid (740 mg, 500 μL, 6.5 mmol) was added. The solution was stirred at room temperature for 1 h. Afterwards, it was concentrated in vacuo (25 ° C, 5 mbar). The residue, a brown viscous oil, was dissolved in dichloromethane (40 mL) and saturated aqueous sodium bicarbonate solution (15 mL) was added. After stirring for 5 min, the phases were separated and the aqueous phase was extracted with dichloromethane (2x15 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was dissolved in dichloromethane (1 mL), MTBE (5 mL) was added and concentrated in vacuo again. After drying in vacuo (50° C., 5 mbar), a pale solid was obtained, which was a crude product. The crude product was purified by chiral preparative HPLC (Chiralpak IE, 250*4.6mm*5 μm, isocratic, (isopropyl alcohol+0.1% triethylamine)/dichloromethane 90/10, flow rate 0.7 mL/min) to obtain the title compound as a white powder (29 mg, 41%). For the purpose of transfer, the material was dissolved in dichloromethane (1 mL) and MTBE (4 mL) and concentrated and dried in vacuo at 50° C./5 mbar. HPLC (method LCMS_ gradient) t _R =1.3 min. ^1HNMR (CDCl ₃ , 400MHz): δ1.64-2.01 (m, 4H), 1.71 (s, 3H), 1.86 (s, 3H), 1.93 (s, 3H), 3.41-3.53 (m, 1H), 3.54-3.63 (m, 1H), 3.64-3. 74 (m, 1H), 4.09 (s, 3H), 7.55 (dd, J=9.4, 9.9Hz, 1H), 8.23 (s, 1H), 8.44 (dd, J=3.0, 8.9Hz, 1H), 9.03 (s, 1H), 10.19 (br s, 1H). MS(ES+)m/z476.2[M+H].

N-(6-((4aR，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺(2BA)N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide (2BA)

步骤1：((4aR，5R，9S)-5-(3-氟-6-(5-甲氧基吡嗪-2-甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-22BA)Step 1: tert-Butyl ((4aR,5R,9S)-5-(3-fluoro-6-(5-methoxypyrazine-2-carboxamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-22BA)

将((4aR，5R，9S)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-17BA，70mg，159μmol)溶解在二氯甲烷(4mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(53mg，70μl，409μmol)，接着加入5-甲氧基吡嗪-2-甲酰氯(对于制备，参见实施例2AB，40mg，232μmol)在二氯甲烷(1.3mL)中的溶液。将反应混合物在室温搅拌3h。然后，加入乙醇(200μl)，将混合物在室温搅拌30min，倒到饱和碳酸氢钠水溶液(15mL)上并且用二氯甲烷(1x30mL，2x20mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度80∶20至100∶0洗脱)纯化，在真空中干燥(50℃，5毫巴)后得到标题化合物，为黄色泡沫(80mg，87％产率)。HPLC(方法LCMS_梯度)t_R＝2.9min。¹HNMR(CDCl₃，400MHz)：δ1.34(s，3H)，1.57(s，9H)，1.79(s，3H)，1.82(s，3H)，1.86-1.95(m，2H)，2.18-2.30(m，1H)，2.53-2.62(m，1H)，3.51(ddd，J＝5.4，8.1，13.4Hz，1H)，3.66(ddd，J＝4.6，4.6，13.2Hz，1H)，3.97(dd，J＝4.7，10.3Hz，1H)，4.08(s，3H)，7.48(dd，J＝8.9，10.5Hz，1H)，8.18(d，J＝1.3Hz，1H)，8.39(dd，J＝3.1，9.0Hz，1H)，9.02(d，J＝1.3Hz，1H)，9.86(br s，1H)，10.80(br s，1H)。MS(ES+)m/z 576.3[M+H]。Tert-butyl ((4aR,5R,9S)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-17BA, 70 mg, 159 μmol) was dissolved in dichloromethane (4 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (53 mg, 70 μl, 409 μmol) was added, followed by a solution of 5-methoxypyrazine-2-carbonyl chloride (for preparation, see Example 2AB, 40 mg, 232 μmol) in dichloromethane (1.3 mL). The reaction mixture was stirred at room temperature for 3 h. Then, ethanol (200 μl) was added, the mixture was stirred at room temperature for 30 min, poured onto saturated aqueous sodium bicarbonate solution (15 mL) and extracted with dichloromethane (1 x 30 mL, 2 x 20 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 80: 20 to 100: 0) and dried in vacuo (50 ° C, 5 mbar) to give the title compound as a yellow foam (80 mg, 87% yield). HPLC (method LCMS_gradient) t _R = 2.9 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.34 (s, 3H), 1.57 (s, 9H), 1.79 (s, 3H), 1.82 (s, 3H), 1.86-1.95 (m, 2H), 2.1 8-2.30 (m, 1H), 2.53-2.62 (m, 1H), 3.51 (ddd, J=5.4, 8.1, 13.4Hz, 1H), 3.66 (ddd, J=4.6 , 4.6, 13.2Hz, 1H), 3.97 (dd, J=4.7, 10.3Hz, 1H), 4.08 (s, 3H), 7.48 (dd, J=8.9, 10.5Hz, 1H), 8.18 (d, J=1.3Hz, 1H), 8.39 (dd, J=3.1, 9.0Hz, 1H), 9.02 (d, J=1.3Hz, 1H), 9.86 (br s, 1H), 10.80 (br s, 1H). MS(ES+)m/z 576.3[M+H].

步骤2：N-(6-((4aR，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺(2BA)Step 2: N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide (2BA)

将((4aR，5R，9S)-5-(3-氟-6-(5-甲氧基吡嗪-2-甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-22BA，80mg，139μmol)溶解在二氯甲烷(4mL)中并且加入三氟乙酸(740mg，500μL，6.5mmol)。将溶液在室温搅拌1h。之后，将其在真空中浓缩(25℃，5毫巴)。将残留物棕色粘性油状物溶解在二氯甲烷(40mL)中，并且加入饱和碳酸氢钠水溶液(15mL)。搅拌5min后，分离相并且将水相用二氯甲烷(2x 15mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物溶解在二氯甲烷(1mL)中，加入MTBE(5mL)并且再次在真空中浓缩，在真空中干燥(50℃，5毫巴)后得到灰白色固体，为粗产物。将粗产物通过手性制备型HPLC(ReprosilChiral NR，250*4.6mm*8μm，等度，正庚烷/(乙醇+0.1％三乙胺)50/50，流速1.0mL/min)纯化，得到标题化合物，为白色粉末(30mg，45％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和MTBE(5mL)中并浓缩并在真空中在50℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.2min。¹H NMR(CDCl₃，400MHz)：δ1.39(s，3H)，1.74(s，3H)，1.79(s，3H)，1.81-1.98(m，2H)，2.06-2.18(m，1H)，2.49-2.59(m，1H)，3.48(ddd，J＝4.6，9.1，13.5Hz，1H)，3.67(ddd，J＝4.7，4.7，13.4Hz，1H)，3.90(dd，J＝4.7，10.3Hz，1H)，4.07(s，3H)，7.45(dd，J＝8.9，10.8Hz，1H)，8.19(d，J＝1.3Hz，1H)，8.32(dd，J＝3.0，8.9Hz，1H)，9.01(d，J＝1.1Hz，1H)，9.94(br s，1H)。MS(ES+)m/z476.3[M+H]。Tert-butyl ((4aR, 5R, 9S)-5-(3-fluoro-6-(5-methoxypyrazine-2-carboxamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-22BA, 80 mg, 139 μmol) was dissolved in dichloromethane (4 mL) and trifluoroacetic acid (740 mg, 500 μL, 6.5 mmol) was added. The solution was stirred at room temperature for 1 h. Afterwards, it was concentrated in vacuo (25 ° C, 5 mbar). The residue, a brown viscous oil, was dissolved in dichloromethane (40 mL) and saturated aqueous sodium bicarbonate solution (15 mL) was added. After stirring for 5 min, the phases were separated and the aqueous phase was extracted with dichloromethane (2 x 15 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was dissolved in dichloromethane (1 mL), MTBE (5 mL) was added and concentrated again in vacuo to give an off-white solid after drying in vacuo (50° C., 5 mbar) as a crude product. The crude product was purified by chiral preparative HPLC (Reprosil Chiral NR, 250*4.6 mm*8 μm, isocratic, n-heptane/(ethanol+0.1% triethylamine) 50/50, flow rate 1.0 mL/min) to give the title compound as a white powder (30 mg, 45%). For transfer purposes, the material was dissolved in dichloromethane (1 mL) and MTBE (5 mL) and concentrated and dried in vacuo at 50° C./5 mbar. HPLC (method LCMS_gradient) t _R =1.2 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.39 (s, 3H), 1.74 (s, 3H), 1.79 (s, 3H), 1.81-1.98 (m, 2H), 2.06-2.18 (m , 1H), 2.49-2.59 (m, 1H), 3.48 (ddd, J=4.6, 9.1, 13.5Hz, 1H), 3.67 (ddd, J=4.7, 4.7, 13.4Hz, 1H), 3.90 (dd, J=4.7, 10.3Hz, 1H), 4.07 (s, 3H), 7.45 (dd, J=8.9, 10.8Hz, 1H ), 8.19 (d, J = 1.3Hz, 1H), 8.32 (dd, J = 3.0, 8.9Hz, 1H), 9.01 (d, J = 1.1Hz, 1H), 9.94 (br s, 1H). MS(ES+)m/z476.3[M+H].

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3，5-二氯吡啶酰胺(3AA)N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridinecarboxamide (3AA)

步骤1：((4aR，5R，9R)-5-(6-(3，5-二氯吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-23AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(3,5-dichloropicolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-23AA)

将3，5-二氯吡啶甲酸(200mg，1.04mmol)悬浮在二氯甲烷(4mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(363mg，250μl，2.86mmol，)以及一滴二甲基甲酰胺和甲苯(1∶3，v/v)的混合物。将混合物在室温搅拌1.5h。然后，将其在真空中在40℃浓缩，将残留物用正庚烷(4mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到3，5-二氯吡啶甲酰氯，为黄色固体(110mg)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-17AA，100mg，228μmol)溶解在二氯甲烷(4mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(75.5mg，100μl，584μmol)，接着加入3，5-二氯吡啶甲酰氯(见上，60mg，285μmol)在二氯甲烷(1.2mL)中的溶液。反应混合物在0-5℃搅拌1.5h。然后，加入乙醇(100μl)，并且将混合物在室温搅拌45min，倒到饱和碳酸氢钠水溶液(15mL)上并且用二氯甲烷(1x 30mL，2x 20mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度60∶40至80∶20洗脱)纯化，在真空中干燥(50℃，5毫巴)后得到标题化合物，为浅黄色泡沫(140mg，99％产率)。HPLC(方法LCMS_梯度)t_R＝3.2min。¹H NMR(CDCl₃，400MHz)：δ1.48(s，9H)，1.79(s，3H)，1.81-2.02(m，3H)，1.85(s，3H)，1.95(s，3H)，2.40(dddd，J＝3.8，12.6，12.6，12.6Hz，1H)，3.36-3.43(m，1H)，3.58-3.66(m，1H)，4.08(dd，J＝3.2，12.4Hz，1H)，7.57(dd，J＝8.9，10.7Hz，1H)，7.95(d，J＝2.1Hz，1H)，8.44(dd，J＝3.1，9.0Hz，1H)，8.57(d，J＝1.9Hz，1H)，10.16(br s，1H，exch)，11.23(br s，1H，exch)。MS(ES+)m/z613.3，615.3&617.3[M+H，2Cl]。3,5-dichloropicolinic acid (200mg, 1.04mmol) is suspended in dichloromethane (4mL), the suspension is cooled to 0-5 ℃ (ice bath) and oxalyl chloride (363mg, 250 μ l, 2.86mmol,) and a drop of dimethylformamide and toluene (1: 3, v/v) are added. The mixture is stirred at room temperature for 1.5h. Then, it is concentrated at 40 ℃ in a vacuum, the residue is processed with n-heptane (4mL) and concentrated again and dried in a vacuum (40 ℃, 5 mbar) to obtain 3,5-dichloropicolinic acid chloride as a yellow solid (110mg). Subsequently, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-17AA, 100 mg, 228 μmol) was dissolved in dichloromethane (4 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (75.5 mg, 100 μl, 584 μmol) was added, followed by a solution of 3,5-dichloropicolinoyl chloride (see above, 60 mg, 285 μmol) in dichloromethane (1.2 mL). The reaction mixture was stirred at 0-5° C. for 1.5 h. Then, ethanol (100 μl) was added and the mixture was stirred at room temperature for 45 min, poured onto saturated aqueous sodium bicarbonate solution (15 mL) and extracted with dichloromethane (1 x 30 mL, 2 x 20 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 60: 40 to 80: 20) and dried in vacuo (50° C., 5 mbar) to give the title compound as a light yellow foam (140 mg, 99% yield). HPLC (method LCMS_gradient) t _R = 3.2 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.48 (s, 9H), 1.79 (s, 3H), 1.81-2.02 (m, 3H), 1.85 (s, 3H), 1.95 ( s, 3H), 2.40 (dddd, J=3.8, 12.6, 12.6, 12.6Hz, 1H), 3.36-3.43 (m, 1H), 3.58-3 .66 (m, 1H), 4.08 (dd, J=3.2, 12.4Hz, 1H), 7.57 (dd, J=8.9, 10.7Hz, 1H), 7.95 ( d, J=2.1Hz, 1H), 8.44 (dd, J=3.1, 9.0Hz, 1H), 8.57 (d, J=1.9Hz, 1H), 10.16 (br s, 1H, exch), 11.23 (br s, 1H, exch). MS(ES+)m/z613.3, 615.3&617.3[M+H, 2Cl].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3，5-二氯吡啶酰胺(3AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridinecarboxamide (3AA)

将((4aR，5R，9R)-5-(6-(3，5-二氯吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-23AA，140mg，228μmol)溶解在二氯甲烷(6mL)中并且加入三氟乙酸(1.78g，1.2mL，15.6mmol)。将溶液在室温搅拌0.5h。之后，将其在真空中浓缩(25℃，5毫巴)。将残留物棕色粘性油状物溶解在二氯甲烷(40mL)中，并且加入饱和碳酸氢钠水溶液(15mL)。搅拌5min后，分离相并且将水相用二氯甲烷(2x 15mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物溶解在二氯甲烷(1mL)中，加入MTBE(5mL)并且再次在真空中浓缩，在真空中干燥(50℃，5毫巴)后得到灰白色固体，为粗产物。将粗产物通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.1％乙酸铵)80/20，流速1.0mL/min)纯化，得到标题化合物，为白色粉末(61mg，52％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和MTBE(4mL)中并浓缩并在真空中在50℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.6min。¹H NMR(CDCl₃，400MHz)：δ1.73-1.88(m，3H)，1.81(s，3H)，1.89(s，3H)，1.90(s，3H)，2.27-2.42(m，1H)，3.37(b d，J＝12.6Hz，1H)，3.52-3.65(m，1H)，3.79(dd，J＝3.1，12.6Hz，1H)，7.51(dd，J＝8.9，10.7Hz，1H)，7.93(d，J＝1.9Hz，1H)，8.39(dd，J＝3.0，8.7Hz，1H)，8.56(d，J＝1.9Hz，1H)，10.17(br s，1H)。MS(ES+)m/z 513.2，515.3&517.3[M+H，2Cl]。Tert-butyl ((4aR,5R,9R)-5-(6-(3,5-dichloropyridinecarboxamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-23AA, 140 mg, 228 μmol) was dissolved in dichloromethane (6 mL) and trifluoroacetic acid (1.78 g, 1.2 mL, 15.6 mmol) was added. The solution was stirred at room temperature for 0.5 h. Afterwards, it was concentrated in vacuo (25 ° C, 5 mbar). The residue, a brown viscous oil, was dissolved in dichloromethane (40 mL) and saturated aqueous sodium bicarbonate (15 mL) was added. The product was stirred for 5 min, and the phase was separated and the aqueous phase was extracted with dichloromethane (2 x 15 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was dissolved in dichloromethane (1 mL), MTBE (5 mL) was added and concentrated in vacuo, and after drying in vacuo (50 ° C, 5 mbar), a pale solid was obtained, which was a crude product. The crude product was purified by chiral preparative HPLC (Chiralpak AD, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.1% ammonium acetate) 80/20, flow velocity 1.0 mL/min) to obtain the title compound, which was a white powder (61 mg, 52%). For the purpose of transfer, the material was dissolved in dichloromethane (1 mL) and MTBE (4 mL) and concentrated and dried in vacuo at 50 ° C/5 mbar. HPLC (method LCMS_ gradient) t _R =1.6 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.73-1.88 (m, 3H), 1.81 (s, 3H), 1.89 (s, 3H), 1.90 (s, 3H), 2.27-2.42 (m, 1H), 3.37 (bd, J=12.6Hz, 1H), 3.52-3.65 (m, 1H), 3.79 (dd, J=3.1, 12.6Hz, 1H), 7.51 (dd, J=8.9, 10.7Hz, 1H), 7.93 (d, J=1.9Hz, 1H), 8.39 (dd, J=3.0, 8.7Hz, 1H), 8.56 (d, J=1.9Hz, 1H), 10.17 (br s, 1H). MS (ES+) m/z 513.2, 515.3 & 517.3 [M+H, 2Cl].

N-(6-((4aS，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(1BB)N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (1BB)

步骤1：((4aS，5R，9S)-5-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-25BB)Step 1: tert-Butyl ((4aS,5R,9S)-5-(6-(5-cyano-3-methylpicolinamido)-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-25BB)

将5-氰基-3-甲基吡啶甲酸(100mg，617μmol)悬浮在二氯甲烷(2mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(145mg，100μL，1.14mmol)以及一滴二甲基甲酰胺和二氯甲烷(1∶3，v/v)的混合物。将混合物在室温搅拌1.5h。然后，将其在真空中浓缩，将残留物用正庚烷(2mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-氰基-3-甲基吡啶甲酰氯，为红色油状物(110mg)。其后，将((4aS，5R，9S)-5-(6-氨基-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-24BB，40mg，72μmol)溶解在二氯甲烷(3mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(37.7mg，50μl，292μmol)，接着加入5-氰基-3-甲基吡啶甲酰氯(见上，21.3mg，118μmol)在二氯甲烷(2.5mL)中的溶液。将反应混合物在0-5℃搅拌1.5h。然后，加入乙醇(50μl)，将混合物在室温搅拌15min，倒到饱和碳酸氢钠水溶液(10mL)上并用二氯甲烷(1x 30mL，2x 20mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。，将粗产物(66mg)在不进一步纯化的情况下用于下一步骤。HPLC(方法7626L05)t_R＝9.7min。5-cyano-3-methylpicolinic acid (100mg, 617μmol) is suspended in dichloromethane (2mL), the suspension is cooled to 0-5 ℃ (ice bath) and add oxalyl chloride (145mg, 100μL, 1.14mmol) and a mixture of dimethylformamide and dichloromethane (1: 3, v/v).The mixture is stirred at room temperature for 1.5h.Then, it is concentrated in a vacuum, residue is handled and concentrated again with n-heptane (2mL) and dried in a vacuum (40 ℃, 5 mbar), obtain 5-cyano-3-methylpicolinic acid chloride, is a red oily substance (110mg). Subsequently, tert-butyl ((4aS,5R,9S)-5-(6-amino-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-24BB, 40 mg, 72 μmol) was dissolved in dichloromethane (3 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (37.7 mg, 50 μl, 292 μmol) was added, followed by a solution of 5-cyano-3-methylpicolinyl chloride (see above, 21.3 mg, 118 μmol) in dichloromethane (2.5 mL). The reaction mixture was stirred at 0-5° C. for 1.5 h. Then, ethanol (50 μl) was added, the mixture was stirred at room temperature for 15 min, poured onto saturated aqueous sodium bicarbonate solution (10 mL) and extracted with dichloromethane (1 x 30 mL, 2 x 20 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The crude product (66 mg) was used in the next step without further purification. HPLC (Method 7626L05) t _R = 9.7 min.

步骤2：N-(6-((4aS，5R，9S)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(1BB)Step 2: N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (1BB)

将((4aS，5R，9S)-5-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟-4-(三乙基甲硅烷基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-25BB，66mg，72μmol)溶解在中二氯甲烷(4mL)中并且加入三氟乙酸(740mg，0.5mL，6.5mmol)。将溶液在室温搅拌1h。之后，将其在真空中浓缩(25℃，5毫巴)。将残留物棕色粘性油状物溶解在二氯甲烷(10mL)中，并且加入饱和碳酸氢钠水溶液(5mL)。搅拌5min后，分离相并且将水相用二氯甲烷(2x 5mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物(70mg)溶解在THF(2mL)和DMF(0.5mL)中。在室温加入乙酸(21mg，20μl，349μmol)和氟化钾(20mg，344μmol)并且将得到的混合物在该温度搅拌2h，接着在60℃搅拌18h。然后，将其冷却至室温并且加入四正丁基氟化铵在四氢呋喃中的溶液(1.0M，150μl，150μmol)中并且将混合物在50℃搅拌4h。之后，将其倒到饱和碳酸氢钠水溶液(10mL)上并且用MTBE(1x 20mL，1x 10mL)萃取。将合并的萃取物用盐水(10mL)洗涤，干燥(硫酸钠)并在真空中浓缩，得到棕色粘性油状物，为粗产物。将粗产物通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.1％乙酸铵)70/30，流速1.0mL/min)纯化，得到标题化合物，为灰白色粉末(15mg，43％总体产率)。为了转移的目的，将材料溶解在二氯甲烷(2mL)和MTBE(8mL)中并浓缩并在真空中在50℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.4min。¹H NMR(CDCl₃，400MHz，主旋转异构体)：δ1.81-1.96(m，2H)，1.92(s，3H)，2.00(s，3H)，2.01(s，3H)，2.08-2.21(m，1H)，2.55-2.64(m，1H)，2.88(s，3H)，3.27-3.37(m，2H)，3.49(dd，J＝3.4，12.8Hz，1H)，7.50(dd，J＝9.1，11.0Hz，1H)，7.96(d，J＝1.1Hz，1H)，8.34(dd，J＝2.8，9.0Hz，1H)，8.80(d，J＝1.3Hz，1H)，10.31(br s，1H，exch)。MS(ES+)m/z 484.3[M+H]。Tert-butyl ((4aS,5R,9S)-5-(6-(5-cyano-3-methylpicolinamido)-3-fluoro-4-(triethylsilyl)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-25BB, 66 mg, 72 μmol) was dissolved in dichloromethane (4 mL) and trifluoroacetic acid (740 mg, 0.5 mL, 6.5 mmol) was added. The solution was stirred at room temperature for 1 h. Afterwards, it was concentrated in vacuo (25° C., 5 mbar). The residue, a brown viscous oil, was dissolved in dichloromethane (10 mL) and saturated aqueous sodium bicarbonate (5 mL) was added. At room temperature, add acetic acid (21mg, 20 μ l, 349 μ mol) and potassium fluoride (20mg, 344 μ mol) and stir the mixture that obtains for 2h in this temperature, then stir 18h at 60 ℃.Then, be cooled to room temperature and add the solution of tetra-n-butylammonium fluoride in tetrahydrofuran (1.0M, 150 μ l, 150 μ mol) and stir the mixture for 4h at 50 ℃.Afterwards, pour it on saturated sodium bicarbonate aqueous solution (10mL) and extract with MTBE (1x 20mL, 1x 10mL). The combined extracts were washed with brine (10 mL), dried (sodium sulfate) and concentrated in vacuo to give a brown viscous oil as a crude product. The crude product was purified by chiral preparative HPLC (Chiralpak AD, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.1% ammonium acetate) 70/30, flow rate 1.0 mL/min) to give the title compound as an off-white powder (15 mg, 43% overall yield). For transfer purposes, the material was dissolved in dichloromethane (2 mL) and MTBE (8 mL) and concentrated and dried in vacuo at 50° C./5 mbar. HPLC (method LCMS_gradient) t _R =1.4 min. ¹ H NMR (CDCl ₃ , 400 MHz, major rotamer): δ 1.81-1.96 (m, 2H), 1.92 (s, 3H), 2.00 (s, 3H), 2.01 (s, 3H), 2.08-2.21 (m, 1H), 2.55-2.64 (m, 1H), 2.88 (s, 3H), 3.27-3.37 (m, 2H), 3.49 (dd, J=3.4, 12.8 Hz, 1H), 7.50 (dd, J=9.1, 11.0 Hz, 1H), 7.96 (d, J=1.1 Hz, 1H), 8.34 (dd, J=2.8, 9.0 Hz, 1H), 8.80 (d, J=1.3 Hz, 1H), 10.31 (br s, 1H, exch). MS(ES+)m/z 484.3[M+H].

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺(4AA)N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide (4AA)

步骤1：((4aR，5R，9R)-5-(3-氟-6-(5-氟-3-甲基吡啶甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-26AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(3-fluoro-6-(5-fluoro-3-methylpicolinamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-26AA)

将5-氟-3-甲基吡啶甲酸(200mg，1.29mmol)悬浮在二氯甲烷(4mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(435mg，300μl，3.43mmol)以及一滴二甲基甲酰胺和甲苯(1∶3，v/v)的混合物。将混合物在室温搅拌1h。然后，将其在真空中在40℃浓缩，将残留物用正庚烷(4mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-氟-3-甲基吡啶甲酰氯，为深棕色油状物(220mg)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-17AA，100mg，228μmol)溶解在二氯甲烷(4mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(75.5mg，100μl，584μmol)，接着加入5-氟-3-甲基吡啶甲酰氯(见上，50mg，288μmol)在二氯甲烷(1mL)中的溶液。将反应混合物在0-5℃搅拌1.5h。然后，加入乙醇(100μl)，将混合物在室温搅拌45min，倒到饱和碳酸氢钠水溶液(15mL)上并且用二氯甲烷(1x 30mL，2x 20mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度60∶40至80∶20洗脱)纯化，在真空中干燥(50℃，5毫巴)后得到标题化合物，为白色泡沫(115mg，88％产率)。HPLC(方法LCMS_梯度)t_R＝3.2min。¹H NMR(CDCl₃，400MHz)：δ1.48(s，9H)，1.79(s，3H)，1.80-2.01(m，3H)，1.85(s，3H)，1.96(d，J＝1.2Hz，3H)，2.31-2.48(m，1H)，2.83(s，3H)，3.35-3.45(m，1H)，3.56-3.68(m，1H)，4.07-4.14(m，1H)，7.40(ddd，J＝0.6，2.7，8.8Hz，1H)，7.55(dd，J＝8.9，10.9Hz，1H)，8.38(d，J＝2.6Hz，1H)，8.41(dd，J＝3.0，8.9Hz，1H)，10.41(br s，1H)，11.23(br s，1H，exch)。MS(ES+)m/z 577.3[M+H]。5-fluoro-3-methylpicolinic acid (200mg, 1.29mmol) is suspended in dichloromethane (4mL), the suspension is cooled to 0-5 ℃ (ice bath) and a mixture of oxalyl chloride (435mg, 300 μ l, 3.43mmol) and a drop of dimethylformamide and toluene (1: 3, v/v) is added. The mixture is stirred at room temperature for 1h. Then, it is concentrated at 40 ℃ in a vacuum, the residue is processed with n-heptane (4mL) and concentrated again and dried in a vacuum (40 ℃, 5 mbar) to obtain 5-fluoro-3-methylpicolinic acid chloride, which is a dark brown oil (220mg). Afterwards, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-17AA, 100 mg, 228 μmol) was dissolved in dichloromethane (4 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (75.5 mg, 100 μl, 584 μmol) was added, followed by a solution of 5-fluoro-3-methylpicolinyl chloride (see above, 50 mg, 288 μmol) in dichloromethane (1 mL). The reaction mixture was stirred at 0-5° C. for 1.5 h. Then, ethanol (100 μl) was added, the mixture was stirred at room temperature for 45 min, poured onto saturated aqueous sodium bicarbonate solution (15 mL) and extracted with dichloromethane (1 x 30 mL, 2 x 20 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 60:40 to 80:20) and dried in vacuo (50° C., 5 mbar) to give the title compound as a white foam (115 mg, 88% yield). HPLC (method LCMS_gradient) t _R = 3.2 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.48 (s, 9H), 1.79 (s, 3H), 1.80-2.01 (m, 3H), 1.85 (s, 3H), 1.96 ( d, J=1.2Hz, 3H), 2.31-2.48 (m, 1H), 2.83 (s, 3H), 3.35-3.45 (m, 1H), 3.56-3.6 8 (m, 1H), 4.07-4.14 (m, 1H), 7.40 (ddd, J=0.6, 2.7, 8.8Hz, 1H), 7.55 (dd, J=8 .9, 10.9Hz, 1H), 8.38 (d, J=2.6Hz, 1H), 8.41 (dd, J=3.0, 8.9Hz, 1H), 10.41 (br s, 1H), 11.23 (br s, 1H, exch). MS(ES+)m/z 577.3[M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺(4AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide (4AA)

将((4aR，5R，9R)-5-(3-氟-6-(5-氟-3-甲基吡啶甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-26AA，110mg，191μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(1.48g，1.0mL，13.0mmol)。将溶液在室温搅拌0.5h。之后，将其在真空中浓缩(25℃，5毫巴)。将残留物棕色粘性油状物溶解在二氯甲烷(40mL)中，并且加入饱和碳酸氢钠水溶液(15mL)。搅拌5min后，分离相并且将水相用二氯甲烷(2x 15mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物溶解在二氯甲烷(1mL)中，加入MTBE(5mL)并且再次在真空中浓缩，在真空中干燥(50℃，5毫巴)后得到灰白色固体，为粗产物。将粗产物通过手性制备型HPLC(Chiralpak AD-H，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.1％三乙胺)75/25，流速1.0mL/min)纯化，得到标题化合物，为白色粉末(39mg，43％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和MTBE(4mL)中并浓缩并在真空中在50℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.5min。¹H NMR(CDCl₃，400MHz)：δ1.71-1.90(m，3H)，1.75(s，3H)，1.85(s，3H)，1.87(s，3H)，2.26-2.41(m，1H)，2.83(s，3H)，3.32-3.40(m，1H)，3.55-3.64(m，1H)，3.81(dd，J＝3.4，12.8Hz，1H)，7.39(dd，J＝2.4，8.9Hz，1H)，7.46(dd，J＝8.9，11.0Hz，1H)，8.33(dd，J＝3.0，8.9Hz，1H)，8.38(d，J＝2.4Hz，1H)，10.38(br s，1H)。MS(ES+)m/z 477.3[M+H]。Tert-butyl ((4aR,5R,9R)-5-(3-fluoro-6-(5-fluoro-3-methylpicolinamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-26AA, 110 mg, 191 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (1.48 g, 1.0 mL, 13.0 mmol) was added. The solution was stirred at room temperature for 0.5 h. Afterwards, it was concentrated in vacuo (25 ° C, 5 mbar). The residue, a brown viscous oil, was dissolved in dichloromethane (40 mL) and saturated aqueous sodium bicarbonate (15 mL) was added. The product was stirred for 5 min, and the phase was separated and the aqueous phase was extracted with dichloromethane (2 x 15 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was dissolved in dichloromethane (1 mL), MTBE (5 mL) was added and concentrated in vacuo, and after drying in vacuo (50 ° C, 5 mbar), an off-white solid was obtained, which was a crude product. The crude product was purified by chiral preparative HPLC (Chiralpak AD-H, 250*4.6mm*5 μm, isocratic, n-heptane/(ethanol+0.1% triethylamine) 75/25, flow rate 1.0 mL/min) to obtain the title compound, which was a white powder (39 mg, 43%). For the purpose of transfer, the material was dissolved in dichloromethane (1 mL) and MTBE (4 mL) and concentrated and dried in vacuo at 50 ° C/5 mbar. HPLC (method LCMS_ gradient) t _R =1.5 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.71-1.90 (m, 3H), 1.75 (s, 3H), 1.85 (s, 3H), 1.87 (s, 3H), 2 .26-2.41(m,1H),2.83(s,3H),3.32-3.40(m,1H),3.55-3.64(m,1H),3.8 1 (dd, J=3.4, 12.8Hz, 1H), 7.39 (dd, J=2.4, 8.9Hz, 1H), 7.46 (dd, J=8.9, 11.0Hz, 1H), 8.33 (dd, J=3.0, 8.9Hz, 1H), 8.38 (d, J=2.4Hz, 1H), 10.38 (br s, 1H). MS(ES+)m/z 477.3[M+H].

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-6-氯-3-甲基咪唑并[1，2-a]吡啶-2-甲酰胺(6AA)N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-6-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide (6AA)

步骤1：((4aR，5R，9R)-5-(6-(6-氯-3-甲基咪唑并[1，2-a]吡啶-2-甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-27AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(6-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-27AA)

将6-氯-3-甲基咪唑并[1，2-a]吡啶-2-甲酸(300mg，1.42mmol)悬浮在中二氯甲烷(4mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(435mg，300μl，3.43mmol)以及一滴二甲基甲酰胺和甲苯(1∶3，v/v)的混合物。将混合物在室温搅拌1h。然后，将其在真空中在40℃浓缩，将残留物用正庚烷(4mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-氟-3-甲基吡啶甲酰氯，为棕色固体(360mg)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-17AA，100mg，228μmol)溶解在二氯甲烷(4mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(113mg，150μl，876μmol)，接着加入6-氯-3-甲基咪唑并[1，2-a]吡啶-2-碳酰氯(见上，80mg，349μmol)在二氯甲烷(1mL)中的溶液。将反应混合物在室温搅拌1.5h。然后，加入乙醇(200μl)，将混合物在室温搅拌30min，倒到饱和碳酸氢钠水溶液(15mL)上并用二氯甲烷(1x 30mL，2x 20mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，80∶20，v/v洗脱)纯化，在真空中干燥(50℃，5毫巴)后得到标题化合物，为白色固体(130mg，90％产率)。HPLC(方法LCMS_梯度)t_R＝3.4min。¹H NMR(CDCl₃，400MHz)：δ1.48(s，9H)，1.75-2.01(m，3H)，1.79(s，3H)，1.85(s，3H)，1.96(2s，3H，旋转异构体)，2.31-2.48(m，1H)，2.88(s，3H)，3.36-3.45(m，1H)，3.57-3.69(m，1H)，4.06-4.14(m，1H)，7.28(dd，J＝1.8，9.7Hz，1H)，7.54(dd，J＝8.9，10.9Hz，1H)，7.61(dd，J＝0.9，9.6Hz，1H)，7.99-8.02(m，1H)，8.43(dd，J＝3.0，8.9Hz，1H)，9.81(brs，1H)，11.23(br s，1H，exch)。MS(ES+)m/z 632.3[M+H]。6-chloro-3-methylimidazo [1,2-a] pyridine-2-carboxylic acid (300mg, 1.42mmol) is suspended in dichloromethane (4mL), suspension is cooled to 0-5 ℃ (ice bath) and add oxalyl chloride (435mg, 300 μ l, 3.43mmol) and a drop of dimethylformamide and toluene (1: 3, v/v) mixture.Mixture is stirred at room temperature for 1h.Then, it is concentrated at 40 ℃ in a vacuum, residue is processed and concentrated again and dried in a vacuum (40 ℃, 5 mbar) with n-heptane (4mL), obtain 5-fluoro-3-methylpicolinyl chloride, be brown solid (360mg). Thereafter, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-17AA, 100 mg, 228 μmol) was dissolved in dichloromethane (4 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (113 mg, 150 μl, 876 μmol) was added, followed by a solution of 6-chloro-3-methylimidazo[1,2-a]pyridine-2-carbonyl chloride (see above, 80 mg, 349 μmol) in dichloromethane (1 mL). The reaction mixture was stirred at room temperature for 1.5 h. Then, ethanol (200 μl) was added, the mixture was stirred at room temperature for 30 min, poured onto saturated aqueous sodium bicarbonate solution (15 mL) and extracted with dichloromethane (1 x 30 mL, 2 x 20 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, 80:20, v/v) and dried in vacuo (50° C., 5 mbar) to give the title compound as a white solid (130 mg, 90% yield). HPLC (Method LCMS_Gradient) t _R = 3.4 min. ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.48 (s, 9H), 1.75-2.01 (m, 3H), 1.79 (s, 3H), 1.85 (s, 3H), 1.96 (2s, 3H, rotamer), 2.31-2.48 (m, 1H), 2.88 (s, 3H), 3.36-3.45 (m, 1H), 3.57-3.69 (m, 1H), 4.06-4. 14 (m, 1H), 7.28 (dd, J=1.8, 9.7Hz, 1H), 7.54 (dd, J=8.9, 10.9Hz, 1H), 7.61 (dd, J=0.9 , 9.6Hz, 1H), 7.99-8.02 (m, 1H), 8.43 (dd, J=3.0, 8.9Hz, 1H), 9.81 (brs, 1H), 11.23 (br s, 1H, exch). MS(ES+)m/z 632.3[M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-6-氯-3-甲基咪唑并[1，2-a]吡啶-2-甲酰胺(6AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-6-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide (6AA)

将((4aR，5R，9R)-5-(6-(6-氯-3-甲基咪唑并[1，2-a]吡啶-2-甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-27AA，130mg，206μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(1.48g，1.0mL，13.0mmol)。将溶液在室温搅拌1h。之后，将其在真空中浓缩(25℃，5毫巴)。将残留物棕色粘性油状物溶解在二氯甲烷(40mL)中，并且加入饱和碳酸氢钠水溶液(15mL)。搅拌5min后，分离相并且将水相用二氯甲烷(2x 15mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物溶解在二氯甲烷(1mL)中，加入MTBE(5mL)并且再次在真空中浓缩，在真空中干燥(50℃，5毫巴)后得到灰白色固体，为粗产物。将粗产物通过手性制备型HPLC(Chiralpak AD-H，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.1％三乙胺)50/50，流速1.0mL/min)纯化，得到标题化合物，为白色粉末(61mg，56％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和MTBE(5mL)中并浓缩并在真空中在50℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.7min。¹H NMR(CDCl₃，400MHz)：δ1.64-1.89(m，3H)，1.73(s，3H)，1.83(s，3H)，1.86(s，3H)，2.26-2.40(m，1H)，2.88(s，3H)，3.31-3.40(m，1H)，3.55-3.66(m，1H)，3.81(dd，J＝3.1，12.7Hz，1H)，7.27(dd，J＝1.9，9.7Hz，1H)，7.45(dd，J＝8.9，11.0Hz，1H)，7.60(d，J＝9.7Hz，1H)，7.99-8.02(m，1H)，8.33(dd，J＝3.0，8.9Hz，1H)，9.79(br s，1H)。MS(ES+)m/z 532.3[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-(6-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-27AA, 130 mg, 206 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (1.48 g, 1.0 mL, 13.0 mmol) was added. The solution was stirred at room temperature for 1 h. Afterwards, it was concentrated in vacuo (25° C., 5 mbar). The residue, a brown viscous oil, was dissolved in dichloromethane (40 mL) and saturated aqueous sodium bicarbonate (15 mL) was added. After stirring for 5 min, the phases were separated and the aqueous phase was extracted with dichloromethane (2 x 15 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was dissolved in dichloromethane (1 mL), MTBE (5 mL) was added and concentrated in vacuo again. After drying in vacuo (50 ° C, 5 mbar), an off-white solid was obtained as a crude product. The crude product was purified by chiral preparative HPLC (Chiralpak AD-H, 250*4.6mm*5 μm, isocratic, n-heptane/(ethanol+0.1% triethylamine) 50/50, flow rate 1.0 mL/min) to obtain the title compound as a white powder (61 mg, 56%). For the purpose of transfer, the material was dissolved in dichloromethane (1 mL) and MTBE (5 mL) and concentrated and dried in vacuo at 50 ° C/5 mbar. HPLC (method LCMS_gradient) t _R =1.7 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.64-1.89 (m, 3H), 1.73 (s, 3H), 1.83 (s, 3H), 1.86 (s, 3H), 2.26- 2.40 (m, 1H), 2.88 (s, 3H), 3.31-3.40 (m, 1H), 3.55-3.66 (m, 1H), 3.81 (dd, J=3 .1, 12.7Hz, 1H), 7.27 (dd, J＝1.9, 9.7Hz, 1H), 7.45 (dd, J＝8.9, 11.0Hz, 1H), 7 .60 (d, J=9.7Hz, 1H), 7.99-8.02 (m, 1H), 8.33 (dd, J=3.0, 8.9Hz, 1H), 9.79 (br s, 1H). MS(ES+)m/z 532.3[M+H].

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺(7AA)N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide (7AA)

步骤1：((4aR，5R，9R)-5-(6-(5-(二氟甲氧基)吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-28AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(5-(difluoromethoxy)picolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-28AA)

将5-(二氟甲氧基)吡啶甲酸(300mg，1.59mmol)悬浮在二氯甲烷(4mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(508mg，350μl，4.0mmol)以及一滴二甲基甲酰胺和甲苯(1∶3，v/v)的混合物。将混合物在室温搅拌1h。然后，将其在真空中在40℃浓缩，将残留物用正庚烷(4mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-(二氟甲氧基)吡啶甲酰氯，为深绿色油状物(330mg)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-17AA，100mg，228μmol)溶解在二氯甲烷(4mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(75.5mg，100μl，584μmol)，接着加入5-(二氟甲氧基)吡啶甲酰氯(见上，65mg，313μmol)在二氯甲烷(1mL)中的溶液。将反应混合物在室温搅拌1.5h。然后，加入乙醇(200μl)，将混合物在室温搅拌30min，倒到饱和碳酸氢钠水溶液(15mL)上并用二氯甲烷(1x 30mL，2x 20mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度60∶40至80∶20，v/v洗脱)纯化，在真空中干燥(55℃，5毫巴)后得到标题化合物，为白色固体(135mg，97％产率)。HPLC(方法LCMS_梯度)t_R＝3.1min。¹H NMR(CDCl₃，400MHz)：δ1.48(s，9H)，1.79(s，3H)，1.81-2.04(m，3H)，1.85(s，3H)，1.96(br s，3H)，2.34-2.47(m，1H)，3.37-3.44(m，1H)，3.58-3.67(m，1H)，4.10(dd，J＝3.5，12.4Hz，1H)，6.67(t，J＝71.7Hz，1H)，7.58(dd，J＝9.0，10.9Hz，1H)，7.70(dd，J＝2.7，8.6Hz，1H)，8.34(d，J＝8.9Hz，1H)，8.45(dd，J＝3.0，8.9Hz，1H)，8.55(d，J＝2.4Hz，1H)，10.26(s，1H)，11.25(br s，1H，exch)。MS(ES+)m/z611.3[M+H]。5-(difluoromethoxy)picolinic acid (300mg, 1.59mmol) is suspended in dichloromethane (4mL), the suspension is cooled to 0-5 ℃ (ice bath) and a mixture of oxalyl chloride (508mg, 350 μl, 4.0mmol) and a drop of dimethylformamide and toluene (1: 3, v/v) is added. The mixture is stirred at room temperature for 1h. Then, it is concentrated at 40 ℃ in a vacuum, the residue is processed with n-heptane (4mL) and concentrated again and dried in a vacuum (40 ℃, 5 mbar) to obtain 5-(difluoromethoxy)picolinic acid chloride as a dark green oil (330mg). Afterwards, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-17AA, 100 mg, 228 μmol) was dissolved in dichloromethane (4 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (75.5 mg, 100 μl, 584 μmol) was added, followed by a solution of 5-(difluoromethoxy)picolinoyl chloride (see above, 65 mg, 313 μmol) in dichloromethane (1 mL). The reaction mixture was stirred at room temperature for 1.5 h. Then, ethanol (200 μl) was added, the mixture was stirred at room temperature for 30 min, poured onto saturated aqueous sodium bicarbonate solution (15 mL) and extracted with dichloromethane (1 x 30 mL, 2 x 20 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 60:40 to 80:20, v/v) to give the title compound as a white solid (135 mg, 97% yield) after drying in vacuo (55° C., 5 mbar). HPLC (Method LCMS_Gradient) t _R =3.1 min. ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.48 (s, 9H), 1.79 (s, 3H), 1.81-2.04 (m, 3H), 1.85 (s, 3H), 1.96 (br s, 3H), 2.34-2.47 (m, 1H), 3.37-3.44 (m, 1H), 3.58-3.67 (m, 1H), 4.10 (dd, J=3.5, 12.4Hz, 1H), 6.67 (t, J=71.7Hz, 1H), 7.58 (dd, J=9.0, 10 .9Hz, 1H), 7.70 (dd, J=2.7, 8.6Hz, 1H), 8.34 (d, J=8.9Hz, 1H), 8.45 (dd, J=3.0, 8.9Hz, 1H), 8.55 (d, J=2.4Hz, 1H), 10.26 (s, 1H), 11.25 (br s, 1H, exch). MS (ES+) m/z 611.3 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺(7AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide (7AA)

将((4aR，5R，9R)-5-(6-(5-(二氟甲氧基)吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-28AA，130mg，213μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(1.48g，1.0mL，13.0mmol)。将溶液在室温搅拌1h。之后，将其在真空中浓缩(25℃，5毫巴)。将残留物，无色粘性油状物，溶解在二氯甲烷(40mL)中，并且加入饱和碳酸氢钠水溶液(15mL)。搅拌5min后，分离相并且将水相用二氯甲烷(2x 15mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物溶解在二氯甲烷(1mL)中，加入MTBE(5mL)并且再次在真空中浓缩，在真空中干燥(50℃，5毫巴)后得到灰白色固体，为粗产物。将粗产物通过手性制备型HPLC(Chiralpak AD-H，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.1％三乙胺)70/30，流速1.0mL/min)纯化，得到标题化合物，为白色粉末(73mg，67％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和MTBE(5mL)中并浓缩并在真空中在50℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.5min。¹H NMR(CDCl₃，400MHz)：δ1.67-1.87(m，3H)，1.73(s，3H)，1.84(s，3H)，1.86(s，3H)，2.27-2.41(m，1H)，3.32-3.40(m，1H)，3.55-3.65(m，1H)，3.81(dd，J＝3.2，12.6Hz，1H)，6.66(t，J＝71.7Hz，1H)，7.48(dd，J＝8.9，11.0Hz，1H)，7.69(dd，J＝2.7，8.6Hz，1H)，8.31-8.38(m，2H)，8.54(d，J＝2.4Hz，1H)，10.24(s，1H)。MS(ES+)m/z 511.3[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-(5-(difluoromethoxy)picolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-28AA, 130 mg, 213 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (1.48 g, 1.0 mL, 13.0 mmol) was added. The solution was stirred at room temperature for 1 h. Afterwards, it was concentrated in vacuo (25 ° C, 5 mbar). The residue, a colorless viscous oil, was dissolved in dichloromethane (40 mL) and saturated aqueous sodium bicarbonate solution (15 mL) was added. After stirring for 5 min, the phases were separated and the aqueous phase was extracted with dichloromethane (2 x 15 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was dissolved in dichloromethane (1 mL), MTBE (5 mL) was added and concentrated in vacuo again. After drying in vacuo (50 ° C, 5 mbar), an off-white solid was obtained as a crude product. The crude product was purified by chiral preparative HPLC (Chiralpak AD-H, 250*4.6mm*5 μm, isocratic, n-heptane/(ethanol+0.1% triethylamine) 70/30, flow rate 1.0 mL/min) to obtain the title compound as a white powder (73 mg, 67%). For the purpose of transfer, the material was dissolved in dichloromethane (1 mL) and MTBE (5 mL) and concentrated and dried in vacuo at 50 ° C/5 mbar. HPLC (method LCMS_gradient) t _R = 1.5 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.67-1.87 (m, 3H), 1.73 (s, 3H), 1.84 (s, 3H), 1.86 (s, 3H), 2.2 7-2.41 (m, 1H), 3.32-3.40 (m, 1H), 3.55-3.65 (m, 1H), 3.81 (dd, J=3.2, 12. 6Hz, 1H), 6.66 (t, J=71.7Hz, 1H), 7.48 (dd, J=8.9, 11.0Hz, 1H), 7.69 (dd, J =2.7, 8.6Hz, 1H), 8.31-8.38 (m, 2H), 8.54 (d, J = 2.4Hz, 1H), 10.24 (s, 1H). MS (ES+) m/z 511.3 [M+H].

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺(8AA)N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide (8AA)

步骤1：((4aR，5R，9R)-5-(3-氟-6-(5-(2，2，3，3-四氟丙氧基)吡啶甲酰胺基)-吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-29AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(3-fluoro-6-(5-(2,2,3,3-tetrafluoropropoxy)picolinamido)-pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-29AA)

将5-(2，2，3，3-四氟丙氧基)吡啶甲酸(200mg，790μmol)悬浮在二氯甲烷(4mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(290mg，200μl，2.28mmol)以及一滴二甲基甲酰胺和甲苯(1∶3，v/v)的混合物。将混合物在室温搅拌1h。然后，将其在真空中在40℃浓缩，将残留物用正庚烷(4mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-(2，2，3，3-四氟丙氧基)吡啶甲酰氯，为黄色浑浊油状物(210mg)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-17AA，70mg，159μmol)溶解在二氯甲烷(4mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(52.9mg，70μl，409μmol)，接着加入5-(2，2，3，3-四氟丙氧基)吡啶甲酰氯(见上，60mg，221μmol)在二氯甲烷(1.3mL)中的溶液。将反应混合物在室温搅拌1.5h。然后，加入乙醇(200μl)，将混合物在室温搅拌30min，倒到饱和碳酸氢钠水溶液(15mL)上并用二氯甲烷(1x 30mL，2x 20mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，50g，用乙酸乙酯/正庚烷，梯度60∶40至80∶20，v/v洗脱)纯化，在真空中干燥(55℃，5毫巴)后得到标题化合物，为白色固体(100mg，93％产率)。HPLC(方法LCMS_梯度)t_R＝3.3min。¹H NMR(CDCl₃，400MHz)：δ1.48(s，9H)，1.79(s，3H)，1.80-2.06(m，3H)，1.85(s，3H)，1.96(br s，3H)，2.41(dddd，J＝3.2，12.1，12.6，12.9Hz，1H)，3.37-3.44(m，1H)，3.63(ddd，J＝3.8，12.1，12.9Hz，1H)，4.10(dd，J＝3.5，12.4Hz，1H)，4.52(br t，J＝11.8Hz，2H)，6.07(tt，J＝4.0，53.0Hz，1H)，7.44(dd，J＝2.8，8.7Hz，1H)，7.57(dd，J＝8.9，10.8Hz，1H)，8.30(dd，J＝0.5，8.6Hz，1H)，8.41(d，J＝2.4Hz，1H)，8.45(dd，J＝3.1，9.0Hz，1H)，10.23(s，1H)，11.24(br s，1H)。MS(ES+)m/z 675.4[M+H]。5-(2,2,3,3-tetrafluoropropoxy)picolinic acid (200mg, 790μmol) is suspended in dichloromethane (4mL), the suspension is cooled to 0-5 ℃ (ice bath) and a mixture of oxalyl chloride (290mg, 200μl, 2.28mmol) and a drop of dimethylformamide and toluene (1: 3, v/v) is added. The mixture is stirred at room temperature for 1h. Then, it is concentrated at 40 ℃ in a vacuum, the residue is processed with n-heptane (4mL) and concentrated again and dried in a vacuum (40 ℃, 5 mbar) to obtain 5-(2,2,3,3-tetrafluoropropoxy)picolinic acid chloride as a yellow turbid oil (210mg). Thereafter, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-17AA, 70 mg, 159 μmol) was dissolved in dichloromethane (4 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (52.9 mg, 70 μl, 409 μmol) was added, followed by a solution of 5-(2,2,3,3-tetrafluoropropoxy)picolinoyl chloride (see above, 60 mg, 221 μmol) in dichloromethane (1.3 mL). The reaction mixture was stirred at room temperature for 1.5 h. The product of 4-nitro- 4- pyridine-2-yl 4-nitropropene was stirred at room temperature for 30 minutes, and the mixture was poured onto a saturated sodium bicarbonate aqueous solution (15 mL) and extracted with dichloromethane (1 x 30 mL, 2 x 20 mL). The combined extracts were dried (sodium sulfate) and concentrated in a vacuum. The crude product was purified by column chromatography (silica gel, 50 g, eluted with ethyl acetate/n-heptane, gradient 60: 40 to 80: 20, v/v), and the title compound was obtained after vacuum drying (55 ° C, 5 mbar). The product was white solid (100 mg, 93% yield). HPLC (method LCMS-gradient) t _R =3.3 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.48 (s, 9H), 1.79 (s, 3H), 1.80-2.06 (m, 3H), 1.85 (s, 3H), 1.96 (br s, 3H), 2.41 (dddd, J=3.2, 12.1, 12.6, 12.9Hz, 1H), 3.37-3.44 (m, 1H), 3.63 (dddd, J=3.8, 12.1, 12.9Hz, 1H), 4.10 (dd, J=3.5, 12.4Hz, 1H), 4.52 (br t, J=11.8Hz, 2H), 6.07 (tt, J=4.0, 53.0Hz, 1H), 7.44 (dd, J=2.8, 8.7Hz, 1H), 7.57 (dd, J=8.9, 10.8Hz, 1H) , 8.30 (dd, J=0.5, 8.6Hz, 1H), 8.41 (d, J=2.4Hz, 1H), 8.45 (dd, J=3.1, 9.0Hz, 1H), 10.23 (s, 1H), 11.24 (br s, 1H). MS(ES+)m/z 675.4[M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺(8AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide (8AA)

将((4aR，5R，9R)-5-(3-氟-6-(5-(2，2，3，3-四氟丙氧基)吡啶甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-29AA，100mg，148μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(1.48g，1.0mL，13.0mmol)。将溶液在室温搅拌1h。之后，将其在真空中浓缩(25℃，5毫巴)。将残留物，无色粘性油状物，溶解在二氯甲烷(40mL)中，并且加入饱和碳酸氢钠水溶液(15mL)。搅拌5min后，分离相并且将水相用二氯甲烷(2x 15mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物溶解在二氯甲烷(1mL)中，加入MTBE(5mL)并且再次在真空中浓缩，在真空中干燥(40℃，5毫巴)得到灰白色固体，为粗产物。将粗产物通过手性制备型HPLC(Chiralpak AD-H，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.1％三乙胺)70/30，流速1.0mL/min)纯化，得到标题化合物，为白色粉末(62mg，73％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和MTBE(5mL)并浓缩并在真空中在50℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.8min。¹H NMR(CDCl₃，400MHz)：δ1.71-1.95(m，3H)，1.74(s，3H)，1.85(s，3H)，1.87(s，3H)，2.28-2.42(m，1H)，3.32-3.41(m，1H)，3.54-3.66(m，1H)，3.78-3.86(m，1H)，4.51(t，J＝11.8Hz，2H)，6.07(tt，J＝4.0，53.0Hz，1H)，7.42(dd，J＝2.7，8.6Hz，1H)，7.47(dd，J＝9.1，11.0Hz，1H)，8.29(d，J＝8.6Hz，1H)，8.35(dd，J＝3.0，8.9Hz，1H)，8.40(d，J＝2.7Hz，1H)，10.21(s，1H)。MS(ES+)m/z 575.2[M+H]。Tert-butyl ((4aR,5R,9R)-5-(3-fluoro-6-(5-(2,2,3,3-tetrafluoropropoxy)picolinamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-29AA, 100 mg, 148 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (1.48 g, 1.0 mL, 13.0 mmol) was added. The solution was stirred at room temperature for 1 h. Afterwards, it was concentrated in vacuo (25 ° C, 5 mbar). The residue, a colorless viscous oil, was dissolved in dichloromethane (40 mL) and saturated aqueous sodium bicarbonate solution (15 mL) was added. After stirring for 5 min, the phases were separated and the aqueous phase was extracted with dichloromethane (2 x 15 mL). The combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was dissolved in dichloromethane (1 mL), MTBE (5 mL) was added and concentrated in vacuo again, and dried in vacuo (40 ° C, 5 mbar) to give an off-white solid as a crude product. The crude product was purified by chiral preparative HPLC (Chiralpak AD-H, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.1% triethylamine) 70/30, flow rate 1.0 mL/min) to give the title compound as a white powder (62 mg, 73%). For the purpose of transfer, the material was dissolved in dichloromethane (1 mL) and MTBE (5 mL) and concentrated and dried in vacuo at 50 ° C/5 mbar. HPLC (method LCMS_gradient) t _R =1.8 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.71-1.95 (m, 3H), 1.74 (s, 3H), 1.85 (s, 3H), 1.87 (s, 3H), 2.28-2.42 (m, 1 H), 3.32-3.41 (m, 1H), 3.54-3.66 (m, 1H), 3.78-3.86 (m, 1H), 4.51 (t, J=11.8Hz, 2H), 6. 07 (tt, J=4.0, 53.0Hz, 1H), 7.42 (dd, J=2.7, 8.6Hz, 1H), 7.47 (dd, J=9.1, 11.0Hz, 1H), 8 .29 (d, J=8.6Hz, 1H), 8.35 (dd, J=3.0, 8.9Hz, 1H), 8.40 (d, J=2.7Hz, 1H), 10.21 (s, 1H). MS(ES+)m/z 575.2[M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(9AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (9AB)

步骤1：((3aS，4R，8R)-4-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-64AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-64AB)

向((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，100.0mg，0.24mmol)在THF(5.0mL)中的溶液中加入5-氰基-3-甲基吡啶甲酸(76.2mg，0.48mmol)，接着加入T3P(763.2mg，1.2mmol，50％于乙酸乙酯中)，和二异丙基乙胺(182.8mg，1.44mmol)。将反应物在70℃搅拌4h。之后，将反应混合物用水(10mL)稀释并用乙酸乙酯(2x 10mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用乙酸乙酯/石油醚2∶1洗脱)纯化，在真空中干燥后得到标题化合物，为黄色固体(100mg，75％产率)。¹H NMR(CDCl₃，400MHz)：δ1.50-1.65(m，1H)，1.67(s，9H)，1.87(s，3H)，1.99(s，3H)，2.06-2.20(m，1H)，2.16(s，3H)，2.89(s，3H)，3.56(dd，J＝7.5，10.5Hz，1H)，3.71(ddd，J＝4.9，10.5，10.5Hz，1H)，4.26-4.35(m，1H)，7.62(dd，J＝8.8，10.3Hz，1H)，8.00(s，1H)，8.51(dd，J＝3.0，8.8Hz，1H)，8.72(s，1H)，10.78(s，1H)，12.53(s，1H)。MS(ES+)m/z 570.2[M+H]。To a solution of tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 100.0 mg, 0.24 mmol) in THF (5.0 mL) was added 5-cyano-3-methylpicolinic acid (76.2 mg, 0.48 mmol), followed by T3P (763.2 mg, 1.2 mmol, 50% in ethyl acetate), and diisopropylethylamine (182.8 mg, 1.44 mmol). The reaction was stirred at 70 ° C for 4 h. Afterwards, the reaction mixture was diluted with water (10 mL) and extracted with ethyl acetate (2 x 10 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated to give the crude product. The crude product was purified by column chromatography (silica gel, eluted with ethyl acetate/petroleum ether 2:1) to give the title compound as a yellow solid (100 mg, 75% yield) after drying in vacuo. ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.50-1.65 (m, 1H), 1.67 (s, 9H), 1.87 (s, 3H), 1.99 (s, 3H), 2.06-2.20 (m, 1H), 2.16 (s, 3H), 2.89 (s, 3H), 3.56 (dd, J=7.5, 10.5 Hz, 1H), 3.71 (ddd, J=10. =4.9, 10.5, 10.5Hz, 1H), 4.26-4.35 (m, 1H), 7.62 (dd, J = 8.8, 10.3Hz, 1H), 8.00 (s, 1H), 8.51 (dd, J=3.0, 8.8Hz, 1H), 8.72 (s, 1H), 10.78 (s, 1H), 12.53 (s, 1H). MS(ES+)m/z 570.2[M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(9AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (9AB)

将((3aS，4R，8R)-4-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-64AB，100mg，0.17mmol)溶解在二氯甲烷(4mL)中并且加入三氟乙酸(1.48g，1.0mL，13.0mmol)。将溶液在室温搅拌4h。将反应混合物用饱和碳酸氢钠水溶液碱化至pH＝7～8，用二氯甲烷(2x 10mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化两次，得到产物。将产物用水(20.0mL)和乙腈(10mL)稀释，将溶液浓缩以去除有机溶剂。将残留的水溶液冻干，得到标题化合物，为白色固体(38mg，46％产率)。¹H NMR(CDCl₃，400MHz)：δ1.62-1.75(m，1H)，1.87(s，3H)，1.97(s，3H)，2.06-2.17(m，1H)，2.10(s，3H)，2.84(s，3H)，3.54(dd，J＝7.8，10.5Hz，1H)，3.72(ddd，J＝5.3，10.5，10.5Hz，1H)，4.24-4.33(m，1H)，7.61(dd，J＝9.0，10.3Hz，1H)，7.96(s，1H)，8.48(dd，J＝3.3，9.0Hz，1H)，8.79(s，1H)，10.86(s，1H)。MS(ES+)m/z470.2[M+H]。Tert-butyl ((3aS,4R,8R)-4-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-64AB, 100 mg, 0.17 mmol) was dissolved in dichloromethane (4 mL) and trifluoroacetic acid (1.48 g, 1.0 mL, 13.0 mmol) was added. The solution was stirred at room temperature for 4 h. The reaction mixture was basified with saturated aqueous sodium bicarbonate to pH = 7-8 and extracted with dichloromethane (2 x 10 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified twice by preparative TLC (dichloromethane/methanol 10:1, UV) to give the product. The product was diluted with water (20.0 mL) and acetonitrile (10 mL), and the solution was concentrated to remove the organic solvent. The residual aqueous solution was lyophilized to give the title compound as a white solid (38 mg, 46% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ1.62-1.75 (m, 1H), 1.87 (s, 3H), 1.97 (s, 3H), 2.06-2.17 (m, 1H), 2.10 (s, 3H), 2.84 (s, 3H), 3.54 (dd, J=7.8, 10.5Hz, 1H), 3.72 (ddd, J=5.3, 10.5, 10.5Hz, 1H), 4.24-4.33 (m, 1H), 7.61 (dd, J=9.0, 10.3Hz, 1H), 7.96 (s, 1H), 8.48 (dd, J=3.3, 9.0Hz, 1H), 8.79 (s, 1H), 10.86 (s, 1H). MS (ES+) m/z 470.2 [M+H].

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(9BA)N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (9BA)

步骤1：((3aR，4R，8S)-4-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-64BA)Step 1: tert-Butyl ((3aR,4R,8S)-4-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-64BA)

向((3aR，4R，8S)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39BA，150.0mg，0.35mmol)在THF(20mL)中的溶液中加入5-氰基-3-甲基吡啶甲酸(85.9mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)，和二异丙基乙胺(266.7mg，2.1mmol)。将反应物在70℃搅拌4h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(2x 10mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用乙酸乙酯/石油醚2∶1洗脱)纯化，在真空中干燥后得到标题化合物，为黄色固体(120mg，60％产率)。¹H NMR(CDCl₃，400MHz)：δ0.85(s，3H)，1.50-1.57(m，9H)，1.68-1.75(m，6H)，2.08-2.21(m，1H)，2.50-2.63(m，1H)，2.80-2.89(m，3H)，3.73-3.89(m，2H)，5.19(dd，J＝7.1，11.1Hz，1H)，7.55(dd，J＝9.1，10.2Hz，1H)，7.91-8.00(m，1H)，8.37-8.49(m，1H)，8.78(d，J＝1.4Hz，1H)，10.48(s，1H)，11.03(br s，1H)。MS(ES+)m/z 570.2[M+H]。To a solution of tert-butyl ((3aR,4R,8S)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39BA, 150.0 mg, 0.35 mmol) in THF (20 mL) was added 5-cyano-3-methylpicolinic acid (85.9 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate), and diisopropylethylamine (266.7 mg, 2.1 mmol). The reaction was stirred at 70 ° C for 4 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (2 x 10 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by column chromatography (silica gel, eluted with ethyl acetate/petroleum ether 2:1) to give the title compound as a yellow solid (120 mg, 60% yield) after drying in vacuo. ¹ H NMR (CDCl ₃ , 400MHz): δ0.85 (s, 3H), 1.50-1.57 (m, 9H), 1.68-1.75 (m, 6H), 2.08-2 .21(m, 1H), 2.50-2.63(m, 1H), 2.80-2.89(m, 3H), 3.73-3.89(m, 2H), 5. 19 (dd, J=7.1, 11.1Hz, 1H), 7.55 (dd, J=9.1, 10.2Hz, 1H), 7.91-8.00 (m, 1H), 8.37-8.49 (m, 1H), 8.78 (d, J=1.4Hz, 1H), 10.48 (s, 1H), 11.03 (br s, 1H). MS(ES+)m/z 570.2[M+H].

步骤2：N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(9BA)Step 2: N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (9BA)

将((3aR，4R，8S)-4-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-64BA，120mg，0.21mmol)溶解在乙腈(20mL)和二氯甲烷(6mL)中。加入二溴化锌(142mg，0.63mmol)并且将溶液在35℃搅拌18h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用二氯甲烷(2x 20mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10_∶1，UV)纯化，得到标题化合物，为白色固体(55mg，57％产率)。¹H NMR(CDCl₃，400MHz)：δ0.99(s，3H)，1.85(2s，6H)，2.15-2.32(m，1H)，2.60-2.71(m，1H)，2.86(s，3H)，3.73-3.88(m，2H)，5.14-5.23(m，1H)，7.62(dd，J＝9.4，9.4Hz，1H)，7.97(s，1H)，8.48(dd，J＝3.0，9.0Hz，1H)，8.82(d，J＝1.5Hz，1H)，10.53(s，1H)。MS(ES+)m/z 470.2[M+H]。Tert-butyl ((3aR,4R,8S)-4-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-64BA, 120 mg, 0.21 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (6 mL). Zinc dibromide (142 mg, 0.63 mmol) was added and the solution was stirred at 35 ° C for 18 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with dichloromethane (2 x 20 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10 _: 1, UV) to give the title compound as a white solid (55 mg, 57% yield). ¹ H NMR (CDCl ₃ , 400 MHz): δ 0.99 (s, 3H), 1.85 (2s, 6H), 2.15-2.32 (m, 1H), 2.60-2.71 (m, 1H), 2.86 (s, 3H), 3.73-3.88 (m, 2H), 5.14-5.23 (m, 1H), 7.62 (dd, J=9.4, 9.4 Hz, 1H), 7.97 (s, 1H), 8.48 (dd, J=3.0, 9.0 Hz, 1H), 8.82 (d, J=1.5 Hz, 1H), 10.53 (s, 1H). MS (ES+) m/z 470.2 [M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺(10AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide (10AB)

步骤1：((3aS，4R，8R)-4-(3-氟-6-(5-氟-3-甲基吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-66AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(3-fluoro-6-(5-fluoro-3-methylpicolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-66AB)

向((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，150mg，0.35mmol)在THF(20mL)中的溶液加入5-氟-3-甲基吡啶甲酸(82mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)，和二异丙基乙胺(267mg，2.1mmol)。将反应物在70℃搅拌4h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并用乙酸乙酯(3x 30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用石油醚/乙酸乙酯1∶1洗脱)纯化，在真空中干燥后得到标题化合物，为黄色固体(130mg，66％产率)。¹H NMR(CDCl₃，400MHz)：δ1.50-1.59(m，1H)，1.66(s，9H)，1.86(s，3H)，1.97(s，3H)，2.06-2.12(m，1H)，2.14(s，3H)，2.84(s，3H)，3.54(dd，J＝7.7，10.7Hz，1H)，3.69(ddd，J＝4.8，10.7，10.7Hz，1H)，4.30(ddd，J＝2.1，7.1，12.0Hz，1H)，7.40(dd，J＝2.1，8.9Hz，1H)，7.58(dd，J＝9.1，10.0Hz，1H)，8.31(d，J＝2.6Hz，1H)，8.49(dd，J＝3.1，8.9Hz，1H)，10.78(s，1H)，12.54(s，1H)。MS(ES+)m/z 563.2[M+H]。To a solution of tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-fluoro-3-methylpicolinic acid (82 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate), and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 70 °C for 4 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3 x 30 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated to give the crude product. The crude product was purified by column chromatography (silica gel, eluted with petroleum ether/ethyl acetate 1:1) to give the title compound as a yellow solid (130 mg, 66% yield) after drying in vacuo. ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.50-1.59 (m, 1H), 1.66 (s, 9H), 1.86 (s, 3H), 1.97 (s, 3H), 2.06-2.12 (m, 1H), 2.14 (s, 3H), 2.84 (s, 3H), 3.54 (dd, J=7.7, 10.7 Hz, 1H), 3.69 (ddd, J=4.8, 10.7, 10.7 Hz, 1H). 1H), 4.30 (ddd, J=2.1, 7.1, 12.0Hz, 1H), 7.40 (dd, J=2.1, 8.9Hz, 1H), 7.58 (dd, J=9.1, 10. 0Hz, 1H), 8.31 (d, J=2.6Hz, 1H), 8.49 (dd, J=3.1, 8.9Hz, 1H), 10.78 (s, 1H), 12.54 (s, 1H). MS(ES+)m/z 563.2[M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺(10AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide (10AB)

将((3aS，4R，8R)-4-(3-氟-6-(5-氟-3-甲基吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-66AB，130mg，0.23mmol)溶解在乙腈(20mL)中并且加入二溴化锌(155mg，0.69mmol)。然后，加入二氯甲烷(5mL)并且将得到的溶液在40℃搅拌18h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用二氯甲烷(2x 20mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到产物。将产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)纯化，然后冻干，得到标题化合物，为白色固体(37mg，34％产率)。¹H NMR(CDCl₃，400MHz)：δ1.65-1.79(m，1H)，1.80(s，3H)，1.93(s，3H)，2.05-2.12(m，1H)，2.06(s，3H)，2.82(s，3H)，3.52(dd，J＝7.6，10.5Hz，1H)，3.71(ddd，J＝5.2，10.5，10.5Hz，1H)，4.25(ddd，J＝2.0，7.0，12.3Hz，1H)，7.40(dd，J＝2.3，8.8Hz，1H)，7.56(dd，J＝9.0，10.3Hz，1H)，8.38(d，J＝2.8Hz，1H)，8.45(dd，J＝3.1，8.9Hz，1H)，10.62(s，1H)。MS(ES+)m/z 463.2[M+H]。Tert-butyl ((3aS,4R,8R)-4-(3-fluoro-6-(5-fluoro-3-methylpicolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-66AB, 130 mg, 0.23 mmol) was dissolved in acetonitrile (20 mL) and zinc dibromide (155 mg, 0.69 mmol) was added. Then, dichloromethane (5 mL) was added and the resulting solution was stirred at 40 ° C for 18 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with dichloromethane (2 x 20 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give the product. The product was purified by preparative HPLC (C18, eluting with 0.05% ammonia/acetonitrile) and then lyophilized to give the title compound as a white solid (37 mg, 34% yield). ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.65-1.79 (m, 1H), 1.80 (s, 3H), 1.93 (s, 3H), 2.05-2.12 (m, 1H), 2.06 (s, 3H), 2.82 (s, 3H), 3.52 (dd, J=7.6, 10.5 Hz, 1H), 3.71 (ddd, J=5.2, 10.5, 10.5 Hz, 1H), 4.25 (ddd, J=2.0, 7.0, 12.3Hz, 1H), 7.40 (dd, J=2.3, 8.8Hz, 1H), 7.56 (dd, J=9 .0, 10.3Hz, 1H), 8.38 (d, J=2.8Hz, 1H), 8.45 (dd, J=3.1, 8.9Hz, 1H), 10.62 (s, 1H). MS(ES+)m/z 463.2[M+H].

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺(10BA)N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide (10BA)

步骤1：((3aR，4R，8S)-4-(3-氟-6-(5-氟-3-甲基吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-66BA)Step 1: tert-Butyl ((3aR,4R,8S)-4-(3-fluoro-6-(5-fluoro-3-methylpicolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-66BA)

向((3aR，4R，8S)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39BA，150mg，0.35mmol)在THF(20mL)中的溶液加入5-氟-3-甲基吡啶甲酸(82mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)，和二异丙基乙胺(267mg，2.1mmol)。将反应物在70℃搅拌4h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并用乙酸乙酯(3x 30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用石油醚/乙酸乙酯1∶1洗脱)纯化，在真空中干燥后得到标题化合物，为黄色固体(130mg，66％产率)。¹H NMR(CDCl₃，400MHz)：δ0.81-0.91(m，3H)，1.49-1.59(m，9H)，1.67-1.76(m，6H)，2.08-2.22(m，1H)，2.52-2.64(m，1H)，2.76-2.87(m，3H)，3.71-3.87(m，2H)，5.21(dd，J＝7.1，11.0Hz，1H)，7.37(dd，J＝2.3，8.8Hz，1H)，7.48-7.57(m，1H)，8.37(d，J＝2.5Hz，1H)，8.44(dd，J＝3.0，8.9Hz，1H)，10.46(s，1H)，11.00(br s，1H)。MS(ES+)m/z 563.2[M+H]。To a solution of tert-butyl ((3aR,4R,8S)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39BA, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-fluoro-3-methylpicolinic acid (82 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate), and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 70 °C for 4 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3 x 30 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated to give the crude product. The crude product was purified by column chromatography (silica gel, eluted with petroleum ether/ethyl acetate 1:1) to afford the title compound after drying in vacuo as a yellow solid (130 mg, 66% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ0.81-0.91 (m, 3H), 1.49-1.59 (m, 9H), 1.67-1.76 (m, 6H), 2.08 -2.22(m, 1H), 2.52-2.64(m, 1H), 2.76-2.87(m, 3H), 3.71-3.87(m, 2H), 5.2 1 (dd, J=7.1, 11.0Hz, 1H), 7.37 (dd, J=2.3, 8.8Hz, 1H), 7.48-7.57 (m, 1H), 8.37 (d, J=2.5Hz, 1H), 8.44 (dd, J=3.0, 8.9Hz, 1H), 10.46 (s, 1H), 11.00 (br s, 1H). MS(ES+)m/z 563.2[M+H].

步骤2：N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺(10BA)Step 2: N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide (10BA)

将((3aR，4R，8S)-4-(3-氟-6-(5-氟-3-甲基吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-66BA，130mg，0.23mmol)溶解在乙腈(20mL)中并且加入二溴化锌(155mg，0.69mmol)。然后，加入二氯甲烷(5mL)并且将得到的溶液在40℃搅拌18h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用二氯甲烷(2x 20mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到标题化合物，为白色固体(50mg，47％产率)。¹H NMR(d4-MeOH，400MHz)：δ0.98(s，3H)，1.77(s，3H)，1.82(s，3H)，2.16-2.28(m，1H)，2.72-2.81(m，1H)，2.77(s，3H)，3.65-3.72(m，1H)，3.78(ddd，J＝4.8，10.5，10.5Hz，1H)，5.37(dd，J＝7.1，11.4Hz，1H)，7.65(dd，J＝2.3，9.5Hz，1H)，7.83(dd，J＝9.0，10.8Hz，1H)，8.43(d，J＝2.3Hz，1H)，8.48(dd，J＝3.3，9.0Hz，1H)。MS(ES+)m/z 463.2[M+H]。Tert-butyl ((3aR,4R,8S)-4-(3-fluoro-6-(5-fluoro-3-methylpicolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-66BA, 130 mg, 0.23 mmol) was dissolved in acetonitrile (20 mL) and zinc dibromide (155 mg, 0.69 mmol) was added. Then, dichloromethane (5 mL) was added and the resulting solution was stirred at 40 ° C for 18 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with dichloromethane (2 x 20 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give the title compound as ^a white solid (50 mg, 47% yield). NMR (d4-MeOH, 400MHz): δ0.98 (s, 3H), 1.77 (s, 3H), 1.82 (s, 3H), 2.16-2.28 (m , 1H), 2.72-2.81 (m, 1H), 2.77 (s, 3H), 3.65-3.72 (m, 1H), 3.78 (ddd, J=4.8, 10 .5, 10.5Hz, 1H), 5.37 (dd, J=7.1, 11.4Hz, 1H), 7.65 (dd, J=2.3, 9.5Hz, 1H), 7. 83 (dd, J=9.0, 10.8Hz, 1H), 8.43 (d, J=2.3Hz, 1H), 8.48 (dd, J=3.3, 9.0Hz, 1H). MS (ES+) m/z 463.2 [M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺(11AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide (11AB)

步骤1：((3aS，4R，8R)-4-(6-(3-氯-5-氟吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-67AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(6-(3-chloro-5-fluoropicolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-67AB)

向((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，150mg，0.35mmol)在THF(20mL)中的溶液中加入3-氯-5-氟吡啶甲酸(92.8mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)，和二异丙基乙胺(267mg，2.1mmol)。将反应物在60℃搅拌16h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(2x 30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用石油醚/乙酸乙酯1∶1洗脱)纯化，在真空中干燥后得到标题化合物，为黄色固体(120mg，58％产率)。¹H NMR(CDCl₃，400MHz)：δ1.53-1.59(m，1H)，1.64(s，9H)，1.86(s，3H)，1.97(s，3H)，2.06-2.12(m，1H)，2.14(s，3H)，3.50-3.58(m，1H)，3.64-3.76(m，1H)，4.29(ddd，J＝2.0，7.2，12.0Hz，1H)，7.60(dd，J＝9.1，10.0Hz，1H)，7.70(dd，J＝2.5，7.7Hz，1H)，8.41(d，J＝2.0Hz，1H)，8.52(dd，J＝3.0，8.9Hz，1H)，10.43-10.63(m，1H)，12.53(s，1H)。MS(ES+)m/z 583.1[M+H]。To a solution of tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 150 mg, 0.35 mmol) in THF (20 mL) was added 3-chloro-5-fluoropicolinic acid (92.8 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate), and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 60 ° C for 16 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (2 x 30 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated to give the crude product. The crude product was purified by column chromatography (silica gel, eluted with petroleum ether/ethyl acetate 1:1) to give the title compound as a yellow solid (120 mg, 58% yield) after drying in vacuo. ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.53-1.59 (m, 1H), 1.64 (s, 9H), 1.86 (s, 3H), 1.97 (s, 3H), 2.06-2.12 (m, 1H), 2.14 (s, 3H), 3.50-3.58 (m, 1H), 3.64-3.76 (m, 1H), 4.29 (ddd, J=2.0, 7. 2, 12.0Hz, 1H), 7.60 (dd, J=9.1, 10.0Hz, 1H), 7.70 (dd, J=2.5, 7.7Hz, 1H), 8.41 ( d, J=2.0Hz, 1H), 8.52 (dd, J=3.0, 8.9Hz, 1H), 10.43-10.63 (m, 1H), 12.53 (s, 1H). MS(ES+)m/z 583.1[M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺(11AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide (11AB)

将((3aS，4R，8R)-4-(6-(3-氯-5-氟吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-67AB，120mg，0.20mmol)溶解在乙腈(20mL)和二氯甲烷(5mL)中。然后，加入二溴化锌(135mg，0.60mmol)并且将得到的溶液在40℃搅拌18h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用二氯甲烷(2x 20mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到产物。将产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)再纯化，然后冻干，得到标题化合物，为白色固体(24mg，24％产率)。¹H NMR(CDCl₃，400MHz)：δ1.63-1.79(m，1H)，1.80(s，3H)，1.93(s，3H)，2.06-2.12(m，1H)，2.06(s，3H)，3.52(dd，J＝7.8，10.5Hz，1H)，3.71(ddd，J＝5.1，10.5，10.5Hz，1H)，4.25(ddd，J＝2.0，7.1，12.4Hz，1H)，7.58(dd，J＝9.0，10.3Hz，1H)，7.69(dd，J＝2.4，7.7Hz，1H)，8.46-8.52(m，2H)，10.38(s，1H)。MS(ES+)m/z 483.1[M+H]。Tert-butyl ((3aS,4R,8R)-4-(6-(3-chloro-5-fluoropicolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-67AB, 120 mg, 0.20 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (5 mL). Zinc dibromide (135 mg, 0.60 mmol) was then added and the resulting solution was stirred at 40 °C for 18 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with dichloromethane (2 x 20 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give the product. The product was further purified by preparative HPLC (C18, eluting with 0.05% ammonia/acetonitrile) and then lyophilized to give the title compound as a white solid (24 mg, 24% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ1.63-1.79 (m, 1H), 1.80 (s, 3H), 1.93 (s, 3H), 2.06-2.12 (m, 1H), 2.06 (s, 3H), 3.52 (dd, J=7.8, 10.5Hz, 1H), 3.71 (ddd, J=5.1, 10.5 , 10.5Hz, 1H), 4.25 (ddd, J=2.0, 7.1, 12.4Hz, 1H), 7.58 (dd, J=9.0, 10.3Hz, 1H), 7.69 (dd, J=2.4, 7.7Hz, 1H), 8.46-8.52 (m, 2H), 10.38 (s, 1H). MS (ES+) m/z 483.1 [M+H].

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺(11BA)N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide (11BA)

步骤1：((3aR，4R，8S)-4-(6-(3-氯-5-氟吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-67BA)Step 1: tert-Butyl ((3aR,4R,8S)-4-(6-(3-chloro-5-fluoropicolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-67BA)

向((3aR，4R，8S)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39BA，150mg，0.35mmol)在THF(20mL)中的溶液中加入3-氯-5-氟吡啶甲酸(92.8mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)，和二异丙基乙胺(267mg，2.1mmol)。将反应物在60℃搅拌16h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(2x 30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用石油醚/乙酸乙酯1∶1洗脱)纯化，在真空中干燥后得到标题化合物，为黄色固体(120mg，58％产率)。¹H NMR(CDCl₃，400MHz)：δ0.85(s，3H)，1.54(s，9H)，1.73(s，6H)，2.08-2.22(m，1H)，2.52-2.63(m，1H)，3.70-3.87(m，2H)，5.19(dd，J＝7.1，11.1Hz，1H)，7.54(dd，J＝9.1，10.2Hz，1H)，7.67(dd，J＝2.4，7.7Hz，1H)，8.42-8.51(m，2H)，10.21(s，1H)，11.03(br s，1H)。MS(ES+)m/z583.1[M+H]。To a solution of tert-butyl ((3aR,4R,8S)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39BA, 150 mg, 0.35 mmol) in THF (20 mL) was added 3-chloro-5-fluoropicolinic acid (92.8 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate), and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 60 ° C for 16 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (2 x 30 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by column chromatography (silica gel, eluted with petroleum ether/ethyl acetate 1:1) to give the title compound as a yellow solid (120 mg, 58% yield) after drying in vacuo. ¹ H NMR (CDCl ₃ , 400MHz): δ0.85 (s, 3H), 1.54 (s, 9H), 1.73 (s, 6H), 2.08-2.22 (m, 1H), 2.52-2.63 (m, 1H), 3.70-3.87 (m, 2H), 5.19 (dd, J =7.1, 11.1Hz, 1H), 7.54 (dd, J = 9.1, 10.2Hz, 1H), 7.67 (dd, J = 2.4, 7.7Hz, 1H), 8.42-8.51 (m, 2H), 10.21 (s, 1H), 11.03 (br s, 1H). MS(ES+)m/z583.1[M+H].

步骤2：N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺(11BA)Step 2: N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide (11BA)

将((3aR，4R，8S)-4-(6-(3-氯-5-氟吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-67BA，120mg，0.20mmol)溶解在乙腈(20mL)和二氯甲烷(5mL)中。然后，加入二溴化锌(135mg，0.60mmol)并且将得到的溶液在40℃搅拌18h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用二氯甲烷(2x 20mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到标题化合物，为白色固体(25.0mg，24％产率)。¹H NMR(d4-MeOH，400MHz)：δ1.07(s，3H)，1.91(s，3H)，1.92(s，3H)，2.40-2.52(m，1H)，2.85-2.93(m，1H)，3.78-3.86(m，1H)，3.87-3.96(m，1H)，5.81(dd，J＝7.5，11.5Hz，1H)，7.92(dd，J＝9.0，10.5Hz，1H)，8.03(dd，J＝2.5，8.3Hz，1H)，8.49(dd，J＝2.9，8.9Hz，1H)，8.58(d，J＝2.5Hz，1H)。MS(ES+)m/z 483.2[M+H]。Tert-butyl ((3aR, 4R, 8S)-4-(6-(3-chloro-5-fluoropicolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-67BA, 120 mg, 0.20 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (5 mL). Zinc dibromide (135 mg, 0.60 mmol) was then added and the resulting solution was stirred at 40 ° C for 18 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate (20 mL) and extracted with dichloromethane (2 x 20 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give the title compound as a white solid (25.0 mg, 24% yield). ^1H NMR (d4-MeOH, 400MHz): δ1.07 (s, 3H), 1.91 (s, 3H), 1.92 (s, 3H), 2.40-2.52 (m, 1H), 2.85-2.93 (m, 1H), 3.78-3.86 (m, 1H), 3.87-3.96 (m, 1H) , 5.81 (dd, J=7.5, 11.5Hz, 1H), 7.92 (dd, J=9.0, 10.5Hz, 1H), 8.03 (dd, J=2.5, 8.3Hz, 1H), 8.49 (dd, J=2.9, 8.9Hz, 1H), 8.58 (d, J=2.5Hz, 1H). MS(ES+)m/z 483.2[M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺(12AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide (12AB)

步骤1：((3aS，4R，8R)-4-(3-氟-6-(5-氯-3-甲基吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-68AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(3-fluoro-6-(5-chloro-3-methylpicolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-68AB)

向((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，150mg，0.35mmol)在THF(20mL)中的溶液中加入5-氯-3-甲基吡啶甲酸(92.8mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(267mg，2.1mmol)。将反应物在70℃搅拌4h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(3x 30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(硅胶，二氯甲烷/乙酸乙酯1∶1，UV)纯化，在真空中干燥后得到标题化合物，为黄色固体(100mg，50％产率)。MS(ES+)m/z 579.2[M+H]。To a solution of tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-chloro-3-methylpicolinic acid (92.8 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 70 ° C for 4 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3 x 30 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated to afford the crude product. The crude product was purified by preparative TLC (silica gel, dichloromethane/ethyl acetate 1:1, UV) to afford the title compound as a yellow solid (100 mg, 50% yield) after drying in vacuo. MS (ES+) m/z 579.2 [M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺(12AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide (12AB)

((3aS，4R，8R)-4-(3-氟-6-(5-氯-3-甲基吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-68AB，100mg，0.17mmol)溶解在乙腈(20mL)和二氯甲烷(1mL)中。然后，加入二溴化锌(100mg，0.45mmol)并且将得到的溶液在40℃搅拌16h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用乙酸乙酯(3x 30mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到产物。将产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)再纯化，然后冻干，得到标题化合物，为白色固体(32mg，40％产率)。¹H NMR(CDCl₃，400MHz)：δ1.66-1.79(m，1H)，1.81(s，3H)，1.94(s，3H)，2.04-2.13(m，1H)，2.06(s，3H)，2.80(s，3H)，3.53(dd，J＝7.5，10.5Hz，1H)，3.72(ddd，J＝5.0，10.5，10.5Hz，1H)，4.26(ddd，J＝2.2，7.2，12.4Hz，1H)，7.57(dd，J＝9.0，10.3Hz，1H)，7.68-7.70(m，1H)，8.44-8.50(m，2H)，10.64(s，1H)。MS(ES+)m/z 479.2[M+H]。Tert-butyl ((3aS,4R,8R)-4-(3-fluoro-6-(5-chloro-3-methylpicolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-68AB, 100 mg, 0.17 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (1 mL). Zinc dibromide (100 mg, 0.45 mmol) was then added and the resulting solution was stirred at 40° C. for 16 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3×30 mL). The combined extracts were dried over sodium sulfate, filtered, and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give the product. The product was repurified by preparative HPLC (C18, eluting with 0.05% ammonia in acetonitrile) and then lyophilized to afford the title compound as a white solid (32 mg, 40% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ1.66-1.79 (m, 1H), 1.81 (s, 3H), 1.94 (s, 3H), 2.04-2.13 (m, 1H), 2.06 (s, 3H), 2.80 (s, 3H), 3.53 (dd, J=7.5, 10.5Hz, 1H), 3.72 (ddd, J=5.0, 10.5, 10.5Hz, 1H), 4.26 (ddd, J=2.2, 7.2, 12.4Hz, 1H), 7.57 (dd, J=9.0, 10.3Hz, 1H), 7.68-7.70 (m, 1H), 8.44-8.50 (m, 2H), 10.64 (s, 1H). MS (ES+) m/z 479.2 [M+H].

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺(12BA)N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide (12BA)

步骤1：((3aR，4R，8S)-4-(3-氟-6-(5-氯-3-甲基吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-68BA)Step 1: tert-Butyl ((3aR,4R,8S)-4-(3-fluoro-6-(5-chloro-3-methylpicolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-68BA)

向((3aR，4R，8S)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39BA，150mg，0.35mmol)在THF(20mL)中的溶液加入5-氯-3-甲基吡啶甲酸(92.4mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(267mg，2.1mmol)。将反应物在70℃搅拌4h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并用乙酸乙酯(3x 30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(硅胶，二氯甲烷/乙酸乙酯1∶1，UV)纯化，在真空中干燥后得到标题化合物，为黄色固体(100mg，50％产率)。MS(ES+)m/z 579.2[M+H]。To a solution of tert-butyl ((3aR,4R,8S)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39BA, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-chloro-3-methylpicolinic acid (92.4 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 70 °C for 4 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3 x 30 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated to give the crude product. The crude product was purified by preparative TLC (silica gel, dichloromethane/ethyl acetate 1:1, UV) to give the title compound after drying in vacuo as a yellow solid (100 mg, 50% yield). MS (ES+) m/z 579.2 [M+H].

步骤2：N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺(12BA)Step 2: N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide (12BA)

将((3aR，4R，8S)-4-(3-氟-6-(5-氯-3-甲基吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-68BA，100mg，0.23mmol)溶解在乙腈(20mL)和二氯甲烷(1mL)中。然后，加入二溴化锌(100mg，0.45mmol)并且将得到的溶液在40℃搅拌16h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用乙酸乙酯(3x 30mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到产物。将产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)再纯化，然后冻干，得到标题化合物，为白色固体(21.3mg，26％产率)。¹H NMR(CDCl₃，400MHz)：δ0.82(s，3H)，1.67(s，3H)，1.69(s，3H)，2.11-2.24(m，1H)，2.51-2.60(m，1H)，2.79(s，3H)，3.66-3.73(m，1H)，3.76-3.84(m，1H)，5.13(dd，J＝7.0，10.8Hz，1H)，7.49(dd，J＝9.4，9.4Hz，1H)，7.66(d，J＝2.0Hz，1H)，8.37(dd，J＝2.3，8.8Hz，1H)，8.49(d，J＝2.3Hz，1H)，10.48(s，1H)。MS(ES+)m/z 479.2[M+H]。Tert-butyl ((3aR,4R,8S)-4-(3-fluoro-6-(5-chloro-3-methylpicolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-68BA, 100 mg, 0.23 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (1 mL). Zinc dibromide (100 mg, 0.45 mmol) was then added and the resulting solution was stirred at 40° C. for 16 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3×30 mL). The combined extracts were dried over sodium sulfate, filtered, and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give the product. The product was repurified by preparative HPLC (C18, eluting with 0.05% ammonia in acetonitrile) and then lyophilized to afford the title compound as a white solid (21.3 mg, 26% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ0.82 (s, 3H), 1.67 (s, 3H), 1.69 (s, 3H), 2.11-2.24 (m, 1H), 2 .51-2.60(m, 1H), 2.79(s, 3H), 3.66-3.73(m, 1H), 3.76-3.84(m, 1H), 5. 13 (dd, J=7.0, 10.8Hz, 1H), 7.49 (dd, J=9.4, 9.4Hz, 1H), 7.66 (d, J=2.0Hz, 1H), 8.37 (dd, J=2.3, 8.8Hz, 1H), 8.49 (d, J=2.3Hz, 1H), 10.48 (s, 1H). MS (ES+) m/z 479.2 [M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(氟甲氧基)吡啶酰胺(13AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)picolinamide (13AB)

步骤1：((3aS，4R，8R)-4-(3-氟-6-(5-(氟甲氧基)吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-69AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(3-fluoro-6-(5-(fluoromethoxy)picolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-69AB)

向((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，150mg，0.35mmol)在THF(20mL)中的溶液中加入5-(氟甲氧基)吡啶甲酸(90.6mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(267mg，2.1mmol)。将反应物在70℃搅拌4h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并用乙酸乙酯(3x 30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(硅胶，二氯甲烷/乙酸乙酯1∶1，UV)纯化，在真空中干燥后得到标题化合物，为黄色固体(0.1g，50％产率)。MS(ES+)m/z 579.2[M+H]。To a solution of tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-(fluoromethoxy)picolinic acid (90.6 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 70 °C for 4 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3 x 30 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated to give the crude product. The crude product was purified by preparative TLC (silica gel, dichloromethane/ethyl acetate 1:1, UV) to give the title compound after drying in vacuo as a yellow solid (0.1 g, 50% yield). MS (ES+) m/z 579.2 [M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(氟甲氧基)吡啶酰胺(13AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)picolinamide (13AB)

将((3aS，4R，8R)-4-(3-氟-6-(5-(氟甲氧基)吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-69AB，100mg，0.17mmol)溶解在乙腈(20mL)和二氯甲烷(1mL)中。然后，加入二溴化锌(100mg，0.45mmol)并且将得到的溶液在40℃搅拌16h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用乙酸乙酯(3x 30mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到产物。将产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)再纯化，然后冻干，得到标题化合物，为白色固体(20mg，25％产率)。¹H NMR(d6-DMSO，400MHz)：δ1.67-1.81(m，1H)，1.95(s，3H)，1.97(s，3H)，2.03(s，3H)，2.12-2.21(m，1H)，3.36-3.44(m，1H)，3.58-3.67(m，1H)，4.89-5.00(m，1H)，6.06(d，J＝53.2Hz，2H)，7.82(dd，J＝2.8，8.8Hz，1H)，8.06(dd，J＝9.3，10.3Hz，1H)，8.23(d，J＝8.8Hz，1H)，8.44(dd，J＝2.8，9.0Hz，1H)，8.50(d，J＝2.8Hz，1H)，10.17(s，1H)，11.04(s，1H)，11.10(s，1H)，11.43(s，1H)。MS(ES+)m/z 479.2[M+H]。Tert-butyl ((3aS,4R,8R)-4-(3-fluoro-6-(5-(fluoromethoxy)picolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-69AB, 100 mg, 0.17 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (1 mL). Zinc dibromide (100 mg, 0.45 mmol) was then added and the resulting solution was stirred at 40° C. for 16 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3×30 mL). The combined extracts were dried over sodium sulfate, filtered, and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give the product. The product was repurified by preparative HPLC (C18, eluted with 0.05% ammonia/acetonitrile) and then lyophilized to give the title compound as a white solid (20 mg, 25% yield). ^1H NMR (d6-DMSO, 400 MHz): δ 1.67-1.81 (m, 1H), 1.95 (s, 3H), 1.97 (s, 3H), 2.03 (s, 3H), 2.12-2.21 (m, 1H), 3.36-3.44 (m, 1H), 3.58-3.67 (m, 1H), 4.89-5.00 (m, 1H), 6.06 (d, J = 53.2 Hz, 2H), 7. 82 (dd, J=2.8, 8.8Hz, 1H), 8.06 (dd, J=9.3, 10.3Hz, 1H), 8.23 (d, J=8.8Hz, 1H), 8.44 (dd, J=2. 8, 9.0Hz, 1H), 8.50 (d, J=2.8Hz, 1H), 10.17 (s, 1H), 11.04 (s, 1H), 11.10 (s, 1H), 11.43 (s, 1H). MS(ES+)m/z 479.2[M+H].

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(氟甲氧基)吡啶酰胺(13BA)N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)picolinamide (13BA)

步骤1：((3aR，4R，8S)-4-(3-氟-6-(5-(氟甲氧基)吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-69BA)Step 1: tert-Butyl ((3aR,4R,8S)-4-(3-fluoro-6-(5-(fluoromethoxy)picolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-69BA)

向((3aR，4R，8S)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39BA，150mg，0.35mmol)在THF(20mL)中的溶液中加入5-(氟甲氧基)吡啶甲酸(90.6mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(267mg，2.1mmol)。将反应物在70℃搅拌4h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并用乙酸乙酯(3x 30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(硅胶，二氯甲烷/乙酸乙酯1∶1，UV)纯化，在真空中干燥后得到标题化合物，为黄色固体(0.1g，50％产率)。¹H NMR(CDCl₃，400MHz)：δ1.51-1.60(m，12H)，1.73(2s，6H)，2.06-2.23(m，1H)，2.59(ddd，J＝5.7，11.3，11.3Hz，1H)，3.67-3.89(m，2H)，5.21(dd，J＝7.2，10.8Hz，1H)，5.82(d，J＝52.0Hz，2H)，7.46-7.66(m，2H)，8.28(d，J＝8.5Hz，1H)，8.43-8.53(m，2H)，10.36(s，1H)，11.02(s，1H)。MS(ES+)m/z 579.2[M+H]。To a solution of tert-butyl ((3aR,4R,8S)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39BA, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-(fluoromethoxy)picolinic acid (90.6 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 70 °C for 4 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3 x 30 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated to give the crude product. The crude product was purified by preparative TLC (silica gel, dichloromethane/ethyl acetate 1:1, UV) to afford the title compound after drying in vacuo as a yellow solid (0.1 g, 50% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ1.51-1.60 (m, 12H), 1.73 (2s, 6H), 2.06-2.23 (m, 1H), 2.59 (ddd, J=5.7, 11.3, 11.3Hz, 1H), 3.67-3.89 (m, 2H), 5.21 (dd, J =7.2, 10.8Hz, 1H), 5.82 (d, J = 52.0Hz, 2H), 7.46-7.66 (m, 2H), 8.28 (d, J = 8.5Hz, 1H), 8.43-8.53 (m, 2H), 10.36 (s, 1H), 11.02 (s, 1H). MS(ES+)m/z 579.2[M+H].

步骤2：N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(氟甲氧基)吡啶酰胺(13BA)Step 2: N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)picolinamide (13BA)

将((3aR，4R，8S)-4-(3-氟-6-(5-(氟甲氧基)吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-69BA，100mg，0.17mmol)溶解在乙腈(20mL)和二氯甲烷(1mL)中。然后，加入二溴化锌(100mg，0.45mmol)并且将得到的溶液在40℃搅拌16h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用乙酸乙酯(3x 30mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到标题化合物，为白色固体(56.0mg，69％产率)。¹H NMR(CDCl₃，400MHz)：δ0.90(s，3H)，1.75(s，3H)，1.76(s，3H)，2.13-2.26(m，1H)，2.56-2.64(m，1H)，3.69-3.77(m，1H)，3.82(ddd，J＝4.9，10.5，10.5Hz，1H)，5.17(dd，J＝7.3，11.3Hz，1H)，5.82(d，J＝53.2Hz，2H)，7.51-7.61(m，1H)，8.29(d，J＝8.5Hz，1H)，8.45(dd，J＝3.0，9.0Hz，1H)，8.50(d，J＝2.5Hz，1H)，10.37(s，1H)。MS(ES+)m/z 479.2[M+H]。Tert-butyl ((3aR,4R,8S)-4-(3-fluoro-6-(5-(fluoromethoxy)picolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-69BA, 100 mg, 0.17 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (1 mL). Zinc dibromide (100 mg, 0.45 mmol) was then added and the resulting solution was stirred at 40° C. for 16 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3×30 mL). The combined extracts were dried over sodium sulfate, filtered, and concentrated to afford the title compound as a white solid (56.0 mg, 69% yield). ^1H NMR (CDCl ₃ , 400MHz): δ0.90 (s, 3H), 1.75 (s, 3H), 1.76 (s, 3H), 2.13-2.26 (m, 1H), 2.56- 2.64(m, 1H), 3.69-3.77(m, 1H), 3.82(ddd, J=4.9, 10.5, 10.5Hz, 1H), 5.17(d d, J＝7.3, 11.3Hz, 1H), 5.82 (d, J＝53.2Hz, 2H), 7.51-7.61 (m, 1H), 8.29 (d, J＝ 8.5Hz, 1H), 8.45 (dd, J=3.0, 9.0Hz, 1H), 8.50 (d, J=2.5Hz, 1H), 10.37 (s, 1H). MS (ES+) m/z 479.2 [M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺(14AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide (14AB)

步骤1：((3aS，4R，8R)-4-(6-(5-(二氟甲氧基)吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-70AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(6-(5-(difluoromethoxy)picolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-70AB)

向((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，150mg，0.35mmol)在THF(20mL)中的溶液中加入5-(二氟甲氧基)吡啶甲酸(100mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(267mg，2.1mmol)。将反应物在60℃搅拌16h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(2x30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用石油醚/乙酸乙酯1∶1洗脱)纯化，在真空中干燥后得到标题化合物，为黄色固体(120mg，57％产率)。MS(ES+)m/z 597.2[M+H]。To a solution of tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-(difluoromethoxy)picolinic acid (100 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 60 ° C for 16 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (2×30 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated to afford the crude product. The crude product was purified by column chromatography (silica gel, eluted with petroleum ether/ethyl acetate 1:1) to afford the title compound as a yellow solid (120 mg, 57% yield) after drying in vacuo. MS (ES+) m/z 597.2 [M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺(14AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide (14AB)

将((3aS，4R，8R)-4-(6-(5-(二氟甲氧基)吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-70AB，120mg，0.20mmol)溶解在乙腈(20mL)和二氯甲烷(5mL)中。然后，加入二溴化锌(135mg，0.60mmol)并且将得到的溶液在40℃搅拌18h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用乙酸乙酯(2x 20mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到产物。将产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)再纯化，然后冻干，得到标题化合物，为白色固体(19mg，19％产率)。¹H NMR(d6-DMSO，400MHz)：δ1.58-1.68(m，1H)，1.62(s，3H)，1.73(s，3H)，1.91(s，3H)，2.04-2.13(m，1H)，3.24-3.31(m，1H)，3.45-3.54(m，1H)，3.92-4.04(m，1H)，7.53(t，J＝72.8Hz，1H)，7.86-7.94(m，1H)，7.95(dd，J＝2.8，8.5Hz，1H)，8.26-8.32(m，2H)，8.30(d，J＝8.5Hz，1H)，8.66-8.68(m，1H)，10.84(s，1H)。MS(ES+)m/z 497.2[M+H]。Tert-butyl ((3aS,4R,8R)-4-(6-(5-(difluoromethoxy)picolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-70AB, 120 mg, 0.20 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (5 mL). Zinc dibromide (135 mg, 0.60 mmol) was then added and the resulting solution was stirred at 40° C. for 18 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (2×20 mL). The combined extracts were dried over sodium sulfate, filtered, and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give the product. The product was repurified by preparative HPLC (C18, eluted with 0.05% ammonia in acetonitrile) and then lyophilized to give the title compound as a white solid (19 mg, 19% yield). ¹ H NMR (d6-DMSO, 400 MHz): δ 1.58-1.68 (m, 1H), 1.62 (s, 3H), 1.73 (s, 3H), 1.91 (s, 3H), 2.04-2.13 (m, 1H), 3.24-3.31 (m, 1H), 3.45-3.54 (m, 1H), 3.92-4.04 ( m, 1H), 7.53 (t, J=72.8Hz, 1H), 7.86-7.94 (m, 1H), 7.95 (dd, J=2.8, 8.5Hz, 1H ), 8.26-8.32 (m, 2H), 8.30 (d, J=8.5Hz, 1H), 8.66-8.68 (m, 1H), 10.84 (s, 1H). MS(ES+)m/z 497.2[M+H].

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺(14BA)N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide (14BA)

步骤1：((3aR，4R，8S)-4-(6-(5-(二氟甲氧基)吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-70BA)Step 1: tert-Butyl ((3aR,4R,8S)-4-(6-(5-(difluoromethoxy)picolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-70BA)

向((3aR，4R，8S)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39BA，150mg，0.35mmol)在THF(20mL)中的溶液中加入5-(二氟甲氧基)吡啶甲酸(100mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(267mg，2.1mmol)。将反应物在60℃搅拌16h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(2x30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用石油醚/乙酸乙酯1∶1洗脱)纯化，在真空中干燥后得到标题化合物，为黄色固体(120mg，57％产率)。¹H NMR(CDCl₃，400MHz)：δ1.68(s，9H)，1.86(s，3H)，1.98(s，3H)，2.15(s，3H)，3.55(dd，J＝7.5，10.7Hz，1H)，3.70(ddd，J＝4.7，10.7，10.7Hz，1H)，4.25-4.33(m，1H)，6.46-6.86(m，1H)，7.61(dd，J＝9.0，10.0Hz，1H)，7.70(dd，J＝2.6，8.7Hz，1H)，8.35(d，J＝8.7Hz，1H)，8.46-8.52(m，2H)，10.69(s，1H)，12.63(s，1H)。MS(ES+)m/z 597.1[M+H]。To a solution of tert-butyl ((3aR,4R,8S)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39BA, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-(difluoromethoxy)picolinic acid (100 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 60 ° C for 16 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (2×30 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by column chromatography (silica gel, eluted with petroleum ether/ethyl acetate 1:1) to give the title compound as a yellow solid (120 mg, 57% yield) after drying in vacuo. ¹ H NMR (CDCl ₃ , 400MHz): δ1.68 (s, 9H), 1.86 (s, 3H), 1.98 (s, 3H), 2.15 (s, 3H), 3.55 (dd, J=7.5, 10.7Hz, 1H), 3.70 (ddd, J=4.7, 10.7, 10.7Hz, 1H), 4.25-4.33 (m, 1H) , 6.46-6.86 (m, 1H), 7.61 (dd, J=9.0, 10.0Hz, 1H), 7.70 (dd, J=2.6, 8.7Hz, 1H), 8.35 (d, J=8.7Hz, 1H), 8.46-8.52 (m, 2H), 10.69 (s, 1H), 12.63 (s, 1H). MS (ES+) m/z 597.1 [M+H].

步骤2：N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺(14BA)Step 2: N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide (14BA)

将((3aR，4R，8S)-4-(6-(5-(二氟甲氧基)吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-70BA，120mg，0.20mmol)溶解在乙腈(20mL)和二氯甲烷(5mL)中。然后，加入二溴化锌(135mg，0.60mmol)并且将得到的溶液在40℃搅拌18h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用乙酸乙酯(2x 20mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到标题化合物，为白色固体(55.0mg，55％产率)。¹H NMR(CDCl₃，400MHz)：δ0.95(s，3H)，1.80(s，3H)，1.83(s，3H)，2.13-2.28(m，1H)，2.57-2.67(m，1H)，3.70-3.88(m，2H)，5.19(dd，J＝7.2，11.4Hz，1H)，6.67(t，J＝71.9Hz，1H)，7.59(dd，J＝9.5，9.5Hz，1H)，7.68(dd，J＝2.5，8.5Hz，1H)，8.32(d，J＝8.8Hz，1H)，8.48(dd，J＝2.9，8.9Hz，1H)，8.55(d，J＝2.5Hz，1H)，10.37(s，1H)。MS(ES+)m/z 497.1[M+H]。Tert-butyl ((3aR, 4R, 8S)-4-(6-(5-(difluoromethoxy)picolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-70BA, 120 mg, 0.20 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (5 mL). Zinc dibromide (135 mg, 0.60 mmol) was then added and the resulting solution was stirred at 40 ° C for 18 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate (20 mL) and extracted with ethyl acetate (2 x 20 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give the title compound as a white solid (55.0 mg, 55% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ0.95 (s, 3H), 1.80 (s, 3H), 1.83 (s, 3H), 2.13-2.28 (m, 1H), 2.57- 2.67 (m, 1H), 3.70-3.88 (m, 2H), 5.19 (dd, J=7.2, 11.4Hz, 1H), 6.67 (t, J=71.9 Hz, 1H), 7.59 (dd, J=9.5, 9.5Hz, 1H), 7.68 (dd, J=2.5, 8.5Hz, 1H), 8.32 (d, J= 8.8Hz, 1H), 8.48 (dd, J=2.9, 8.9Hz, 1H), 8.55 (d, J=2.5Hz, 1H), 10.37 (s, 1H). MS (ES+) m/z 497.1 [M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺(15AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide (15AB)

步骤1：((3aS，4R，8R)-4-(3-氟-6-(5-(2，2，3，3-四氟丙氧基)吡啶甲酰胺基)-吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-71AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(3-fluoro-6-(5-(2,2,3,3-tetrafluoropropoxy)picolinamido)-pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-71AB)

向((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，150mg，0.35mmol)在THF(20mL)中的溶液中加入5-(2，2，3，3-四氟丙氧基)吡啶甲酸(134mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(267mg，2.1mmol)。将反应物在70℃搅拌18h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(2x 10mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用石油醚/乙酸乙酯1∶2洗脱)纯化，在真空中干燥后得到纯化的产物。通过在石油醚(10mL)和二氯甲烷(2mL)中在15℃搅拌该材料1h，获得纯的标题化合物。将悬浮液过滤，并在真空中干燥，得到标题化合物，为白色固体(80mg，34％产率)。¹H NMR(CDCl₃，400MHz)：δ1.51-1.57(m，1H)，1.69(s，9H)，1.86(s，3H)，1.98(s，3H)，2.09(d，J＝7.2Hz，1H)，2.15(s，3H)，3.49-3.59(m，1H)，3.64-3.75(m，1H)，4.31(d，J＝6.8Hz，1H)，4.50(t，J＝11.8Hz，2H)，5.89-6.26(m，1H)，7.43(d，J＝8.3Hz，1H)，7.60(dd，J＝9.5Hz，1H)，8.32(d，J＝2.6Hz，2H)，8.44-8.59(m，1H)，10.38-10.71(m，1H)，12.38-12.74(m，1H)。MS(ES+)m/z661.2[M+H]。To a solution of tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-(2,2,3,3-tetrafluoropropoxy)picolinic acid (134 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 70 ° C for 18 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (2 x 10 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated to afford the crude product. The crude product was purified by column chromatography (silica gel, eluted with petroleum ether/ethyl acetate 1:2) and dried in vacuo to afford the purified product. The pure title compound was obtained by stirring the material in petroleum ether (10 mL) and dichloromethane (2 mL) at 15°C for 1 h. The suspension was filtered and dried in vacuo to afford the title compound as a white solid (80 mg, 34% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ1.51-1.57 (m, 1H), 1.69 (s, 9H), 1.86 (s, 3H), 1.98 (s, 3H), 2.09 (d, J=7.2 Hz, 1H), 2.15 (s, 3H), 3.49-3.59 (m, 1H), 3.64-3.75 (m, 1H), 4.31 (d, J=6.8Hz, 1H), 4.5 0 (t, J=11.8Hz, 2H), 5.89-6.26 (m, 1H), 7.43 (d, J=8.3Hz, 1H), 7.60 (dd, J=9.5Hz, 1H) , 8.32 (d, J=2.6Hz, 2H), 8.44-8.59 (m, 1H), 10.38-10.71 (m, 1H), 12.38-12.74 (m, 1H). MS(ES+)m/z661.2[M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺(15AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide (15AB)

将((3aS，4R，8R)-4-(3-氟-6-(5-(2，2，3，3-四氟丙氧基)吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-71AB，70mg，0.1mmol)溶解在乙腈(10mL)和二氯甲烷(5mL)中。然后，加入二溴化锌(67.5mg，0.3mmol)并且将得到的溶液在35℃搅拌16h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用二氯甲烷(2x 20mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)纯化，然后冻干，得到标题化合物，为白色固体(17mg，29％产率)。¹H NMR(CDCl₃，400MHz)：δ1.65-1.81(m，1H)，1.81(s，3H)，1.94(s，3H)，2.05-2.14(m，1H)，2.07(s，3H)，3.49-3.57(m，1H)，3.67-3.77(m，1H)，4.21-4.30(m，1H)，4.52(t，J＝11.8Hz，2H)，6.09(tt，J＝4.0，52.8Hz，1H)，7.40-7.47(m，1H)，7.58(dd，J＝9.5，10.0Hz，1H)，8.29(d，J＝8.5Hz，1H)，8.40(s，1H)，8.49(dd，J＝2.8，8.8Hz，1H)，10.45(s，1H)。MS(ES+)m/z 561.1[M+H]。Tert-butyl ((3aS,4R,8R)-4-(3-fluoro-6-(5-(2,2,3,3-tetrafluoropropoxy)picolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-71AB, 70 mg, 0.1 mmol) was dissolved in acetonitrile (10 mL) and dichloromethane (5 mL). Zinc dibromide (67.5 mg, 0.3 mmol) was then added and the resulting solution was stirred at 35 °C for 16 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with dichloromethane (2 x 20 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative HPLC (C18, eluting with 0.05% ammonia in acetonitrile) and then lyophilized to afford the title compound as a white solid (17 mg, 29% yield). ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.65-1.81 (m, 1H), 1.81 (s, 3H), 1.94 (s, 3H), 2.05-2.14 (m, 1H), 2.07 (s, 3H), 3.49-3.57 (m, 1H), 3.67-3.77 (m, 1H), 4.21-4.30 (m, 1H), 4.52 (t, J=11.8H). z, 2H), 6.09 (tt, J=4.0, 52.8Hz, 1H), 7.40-7.47 (m, 1H), 7.58 (dd, J=9.5, 10.0Hz, 1 H), 8.29 (d, J=8.5Hz, 1H), 8.40 (s, 1H), 8.49 (dd, J=2.8, 8.8Hz, 1H), 10.45 (s, 1H). MS(ES+)m/z 561.1[M+H].

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺(15BA)N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide (15BA)

步骤1：((3aR，4R，8S)-4-(3-氟-6-(5-(2，2，3，3-四氟丙氧基)吡啶甲酰胺基)-吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-71BA)Step 1: tert-Butyl ((3aR,4R,8S)-4-(3-fluoro-6-(5-(2,2,3,3-tetrafluoropropoxy)picolinamido)-pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-71BA)

向((3aR，4R，8S)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39BA，150mg，0.35mmol)在THF(20mL)中的溶液中加入5-(2，2，3，3-四氟丙氧基)吡啶甲酸(134mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(267mg，2.1mmol)。将反应物在70℃搅拌16h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(2x10mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用石油醚/乙酸乙酯1∶1洗脱)纯化，在真空中干燥后得到标题化合物，为黄色固体(100mg，43％产率)。MS(ES+)m/z 661.2[M+H]。To a solution of tert-butyl ((3aR,4R,8S)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39BA, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-(2,2,3,3-tetrafluoropropoxy)picolinic acid (134 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 70 ° C for 16 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (2×10 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated to afford the crude product. The crude product was purified by column chromatography (silica gel, eluted with petroleum ether/ethyl acetate 1:1) to afford the title compound as a yellow solid (100 mg, 43% yield) after drying in vacuo. MS (ES+) m/z 661.2 [M+H].

步骤2：N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺(15BA)Step 2: N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide (15BA)

将((3aR，4R，8S)-4-(3-氟-6-(5-(2，2，3，3-四氟丙氧基)吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-71BA，100mg，0.16mmol)溶解在乙腈(15mL)和二氯甲烷(8mL)中。然后，加入二溴化锌(111mg，0.5mmol)并且将得到的溶液在35℃搅拌18h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用二氯甲烷(2x20mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)，接着制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到标题化合物，为白色固体(19.6mg，23％产率)。¹H NMR(CDCl₃，400MHz)：δ1.00(s，3H)，1.85(2s，6H)，2.17-2.30(m，1H)，2.60-2.71(m，1H)，3.74-3.89(m，2H)，4.52(t，J＝11.8Hz，2H)，5.23(dd，J＝7.2，11.4Hz，1H)，6.08(tt，J＝4.3，52.9Hz，1H)，7.43(dd，J＝2.8，8.8Hz，1H)，7.60(dd，J＝9.3，9.8Hz，1H)，8.29(d，J＝8.8Hz，1H)，8.41(d，J＝2.8Hz，1H)，8.51(dd，J＝2.7，8.9Hz，1H)，10.36(s，1H)。MS(ES+)m/z 561.1[M+H]。Tert-butyl ((3aR, 4R, 8S)-4-(3-fluoro-6-(5-(2,2,3,3-tetrafluoropropoxy)picolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-71BA, 100 mg, 0.16 mmol) was dissolved in acetonitrile (15 mL) and dichloromethane (8 mL). Zinc dibromide (111 mg, 0.5 mmol) was then added and the resulting solution was stirred at 35 ° C for 18 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate (20 mL) and extracted with dichloromethane (2x20 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative HPLC (C18, eluting with 0.05% ammonia/acetonitrile) followed by preparative TLC (dichloromethane/methanol 10:1, UV) to afford the title compound as a white solid (19.6 mg, 23% yield). ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.00 (s, 3H), 1.85 (2s, 6H), 2.17-2.30 (m, 1H), 2.60-2.71 (m, 1H), 3.74-3.89 (m, 2H), 4.52 (t, J=11.8 Hz, 2H), 5.23 (dd, J=7.2, 11.4 Hz, 1H), 6.08 (tt, J= 4.3, 52.9Hz, 1H), 7.43 (dd, J=2.8, 8.8Hz, 1H), 7.60 (dd, J=9.3, 9.8Hz, 1H), 8.29 ( d, J=8.8Hz, 1H), 8.41 (d, J=2.8Hz, 1H), 8.51 (dd, J=2.7, 8.9Hz, 1H), 10.36 (s, 1H). MS(ES+)m/z 561.1[M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺(16AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide (16AB)

步骤1：((3aS，4R，8R)-4-(3-氟-6-(5-甲氧基吡嗪-2-甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-72AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(3-fluoro-6-(5-methoxypyrazine-2-carboxamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-72AB)

向((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，150mg，0.35mmol)在THF(20mL)中的溶液中加入5-甲氧基吡嗪-2-甲酸(90mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(267mg，2.1mmol)。将反应物在70℃搅拌4h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(3x30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(硅胶，二氯甲烷/乙酸乙酯1∶1，UV)纯化，在真空中干燥后得到标题化合物，为黄色固体(100mg，50％产率)。¹H NMR(CDCl₃，400MHz)：δ1.62-2.20(m，24H)，4.05-4.14(m，4H)，4.28(dd，J＝6.8，10.4Hz，1H)，7.54-7.65(m，1H)，8.12(s，1H)，8.48(dd，J＝2.9，8.9Hz，1H)，9.03(s，1H)，10.37(s，1H)，12.60(s，1H)。MS(ES+)m/z 562.2[M+H]。To a solution of tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-methoxypyrazine-2-carboxylic acid (90 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 70 ° C for 4 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3×30 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (silica gel, dichloromethane/ethyl acetate 1:1, UV) to give the title compound as a yellow solid (100 mg, 50% yield) after drying in vacuo. ^1H NMR (CDCl ₃ , 400 MHz): δ 1.62-2.20 (m, 24H), 4.05-4.14 (m, 4H), 4.28 (dd, J=6.8, 10.4 Hz, 1H), 7.54-7.65 (m, 1H), 8.12 (s, 1H), 8.48 (dd, J=2.9, 8.9 Hz, 1H), 9.03 (s, 1H), 10.37 (s, 1H), 12.60 (s, 1H). MS (ES+) m/z 562.2 [M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺(16AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide (16AB)

将((3aS，4R，8R)-4-(3-氟-6-(5-甲氧基吡嗪-2-甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-72AB，100mg，0.17mmol)溶解在乙腈(20mL)和二氯甲烷(1mL)中。加入二溴化锌(100mg，0.45mmol)并将溶液在40℃搅拌16h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用乙酸乙酯(3x30mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到黄色固体，为产物。将产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)再纯化，然后冻干，得到标题化合物，为白色固体(23mg，28％产率)。¹H NMR(CDCl₃，400MHz)：δ1.65-1.78(m，1H)，1.81(s，3H)，1.93(s，3H)，2.04-2.13(m，1H)，2.06(s，3H)，3.52(dd，J＝7.7，10.7Hz，1H)，3.71(ddd，J＝5.0，10.5，10.5Hz，1H)，4.09(s，3H)，4.25(ddd，J＝2.0，6.8，12.1Hz，1H)，7.59(dd，J＝8.9，10.4Hz，1H)，8.23(d，J＝1.3Hz，1H)，8.48(dd，J＝3.1，8.9Hz，1H)，9.03(d，J＝1.2Hz，1H)，10.19(s，1H)。MS(ES+)m/z 462.2[M+H]。Tert-butyl ((3aS,4R,8R)-4-(3-fluoro-6-(5-methoxypyrazine-2-carboxamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-72AB, 100 mg, 0.17 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (1 mL). Zinc dibromide (100 mg, 0.45 mmol) was added and the solution was stirred at 40 °C for 16 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3 x 30 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give a yellow solid as the product. The product was repurified by preparative HPLC (C18, eluting with 0.05% ammonia/acetonitrile) and then lyophilized to give the title compound as a white solid (23 mg, 28% yield). ^1H NMR (CDCl ₃ , 400 MHz): δ 1.65-1.78 (m, 1H), 1.81 (s, 3H), 1.93 (s, 3H), 2.04-2.13 (m, 1H), 2.06 (s, 3H), 3.52 (dd, J = 7.7, 10.7 Hz, 1H), 3.71 (ddd, J = 5.0, 10.5, 10.5 Hz, 1H), 4.09 (m, 1H), 5.84 (ddd, J = 5.0, 10.5, 10.5 Hz, 1H), 4.70 (ddd, J = 5.0, 10.5, 10.5 Hz, 1H), 4.59 (m, 1H), 4.93 (s, 3H), 5.71 (ddd, J = 5.0, 10.5, 10.5 Hz, 1H), 4.89 (m, 1H), 4.97 (m, 1H), 4.10 (m, 1H), 4.57 (s, 3H), 4.11 (m, 1H), 4.59 (m, 1H), 4. (s, 3H), 4.25 (ddd, J=2.0, 6.8, 12.1Hz, 1H), 7.59 (dd, J=8.9, 10.4Hz, 1H), 8.23 (d , J=1.3Hz, 1H), 8.48 (dd, J=3.1, 8.9Hz, 1H), 9.03 (d, J=1.2Hz, 1H), 10.19 (s, 1H). MS(ES+)m/z 462.2[M+H].

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺(16BA)N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide (16BA)

步骤1：((3aR，4R，8S)-4-(3-氟-6-(5-甲氧基吡嗪-2-甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-72BA)Step 1: tert-Butyl ((3aR,4R,8S)-4-(3-fluoro-6-(5-methoxypyrazine-2-carboxamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-72BA)

向((3aR，4R，8S)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39BA，150mg，0.35mmol)在THF(20mL)中的溶液中加入5-甲氧基吡嗪-2-甲酸(90mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(267mg，2.1mmol)。将反应物在70℃搅拌4h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(3x30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(硅胶，二氯甲烷/乙酸乙酯1∶1，UV)纯化，在真空中干燥后得到标题化合物，为黄色固体(100mg，50％产率)。¹H NMR(CDCl₃，400MHz)：δ0.87(s，3H)，1.52-1.60(m，9H)，1.73(2s，6H)，2.09-2.21(m，1H)，2.54-2.62(m，1H)，3.72-3.87(m，2H)，4.08(s，3H)，5.19(dd，J＝7.2，10.9Hz，1H)，7.48-7.60(m，1H)，8.21(d，J＝1.4Hz，1H)，8.47(dd，J＝3.0，8.9Hz，1H)，9.02(d，J＝1.3Hz，1H)，10.04(s，1H)，11.02(s，1H)。MS(ES+)m/z 562.2[M+H]。To a solution of tert-butyl ((3aR,4R,8S)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39BA, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-methoxypyrazine-2-carboxylic acid (90 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 70 ° C for 4 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3×30 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (silica gel, dichloromethane/ethyl acetate 1:1, UV) to afford the title compound after drying in vacuo as a yellow solid (100 mg, 50% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ0.87 (s, 3H), 1.52-1.60 (m, 9H), 1.73 (2s, 6H), 2.09-2.21 (m, 1H), 2.54-2.62 (m, 1H), 3.72-3.87 (m, 2H), 4.08 (s, 3H), 5.19 (dd, J =7.2, 10.9Hz, 1H), 7.48-7.60 (m, 1H), 8.21 (d, J = 1.4Hz, 1H), 8.47 (dd, J = 3.0, 8.9Hz, 1H), 9.02 (d, J = 1.3Hz, 1H), 10.04 (s, 1H), 11.02 (s, 1H). MS (ES+) m/z 562.2 [M+H].

步骤2：N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺(16BA)Step 2: N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide (16BA)

将((3aR，4R，8S)-4-(3-氟-6-(5-甲氧基吡嗪-2-甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-72BA，100mg，0.17mmol)溶解在乙腈(20mL)和二氯甲烷(1mL)中。加入二溴化锌(100mg，0.45mmol)并将溶液在40℃搅拌16h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用乙酸乙酯(3x30mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到黄色固体，为产物。将产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)再纯化，然后冻干，得到标题化合物，为白色固体(15mg，18％产率)。¹H NMR(CDCl₃，400MHz)：δ0.82(s，3H)，1.68(s，3H)，1.70(s，3H)，2.11-2.24(m，1H)，2.52-2.60(m，1H)，3.67-3.74(m，1H)，3.80(ddd，J＝4.7，10.5，10.5Hz，1H)，4.08(s，3H)，5.11(dd，J＝7.3，11.3Hz，1H)，7.51(dd，J＝9.0，10.3Hz，1H)，8.23(d，J＝1.0Hz，1H)，8.40(dd，J＝2.9，8.9Hz，1H)，9.02(d，J＝1.3Hz，1H)，10.06(br s，1H)。MS(ES+)m/z 462.2[M+H]。Tert-butyl ((3aR,4R,8S)-4-(3-fluoro-6-(5-methoxypyrazine-2-carboxamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-72BA, 100 mg, 0.17 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (1 mL). Zinc dibromide (100 mg, 0.45 mmol) was added and the solution was stirred at 40 ° C for 16 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3x30 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give a yellow solid as the product. The product was repurified by preparative HPLC (C18, eluted with 0.05% ammonia/acetonitrile) and then lyophilized to give the title compound as a white solid (15 mg, 18% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ0.82 (s, 3H), 1.68 (s, 3H), 1.70 (s, 3H), 2.11-2.24 (m, 1H), 2.52 -2.60 (m, 1H), 3.67-3.74 (m, 1H), 3.80 (ddd, J=4.7, 10.5, 10.5Hz, 1H), 4.08 (s, 3H), 5.11 (dd, J=7.3, 11.3Hz, 1H), 7.51 (dd, J=9.0, 10.3Hz, 1H), 8.23 (d , J=1.0Hz, 1H), 8.40 (dd, J=2.9, 8.9Hz, 1H), 9.02 (d, J=1.3Hz, 1H), 10.06 (br s, 1H). MS(ES+)m/z 462.2[M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺(25AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide (25AB)

步骤1：((3aS，4R，8R)-4-(6-(3-氯-5-氰基吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-65AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(6-(3-chloro-5-cyanopicolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-65AB)

向((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，150.0mg，0.35mmol)在THF(15mL)中的溶液中加入5-氰基-3-氯吡啶甲酸(96.5mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(266.7mg，2.1mmol)。将反应物在70℃搅拌16h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(2x10mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用乙酸乙酯/石油醚1∶1洗脱)纯化，在真空中干燥后得到标题化合物，为黄色固体(105mg，50％产率)。¹H NMR(CDCl₃，400MHz)：δ1.61-1.70(m，10H)，1.87(s，3H)，1.98(s，3H)，2.10-2.31(m，1H)，2.15(s，3H)，3.50-3.60(m，1H)，3.66-3.76(m，1H)，4.28-4.35(m，1H)，7.59-7.68(m，1H)，8.23(s，1H)，8.49-8.56(m，1H)，8.73-8.78(m，1H)，10.40-10.57(m，1H)，12.41-12.62(m，1H)。MS(ES+)m/z 590.2[M+H]。To a solution of tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 150.0 mg, 0.35 mmol) in THF (15 mL) was added 5-cyano-3-chloropicolinic acid (96.5 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (266.7 mg, 2.1 mmol). The reaction was stirred at 70 ° C for 16 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (2×10 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by column chromatography (silica gel, eluted with ethyl acetate/petroleum ether 1:1) to give the title compound as a yellow solid (105 mg, 50% yield) after drying in vacuo. ¹ H NMR (CDCl ₃ , 400MHz): δ1.61-1.70 (m, 10H), 1.87 (s, 3H), 1.98 (s, 3H), 2.10-2.31 (m, 1H), 2.15 (s, 3H), 3.50-3.60 (m, 1H), 3.66-3.76 (m, 1H), 4 .28-4.35 (m, 1H), 7.59-7.68 (m, 1H), 8.23 (s, 1H), 8.49-8.56 (m, 1H), 8.73-8.78 (m, 1H), 10.40-10.57 (m, 1H), 12.41-12.62 (m, 1H). MS (ES+) m/z 590.2 [M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺(25AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopicinamide (25AB)

将((3aS，4R，8R)-4-(6-(3-氯-5-氰基吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-65AB，100mg，0.17mmol)溶解在二氯甲烷(10mL)中并且加入三氟乙酸(194mg，1.7mmol)。将溶液在室温搅拌4h。将反应混合物用饱和碳酸氢钠水溶液碱化至pH＝7～8，用二氯甲烷(2x10mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到产物。将产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)再纯化，然后冻干，得到标题化合物，为白色固体(18mg，22％产率)。¹H NMR(CDCl₃，400MHz)：δ1.51-1.67(m，1H)，1.79(s，3H)，2.07(s，3H)，2.17-2.26(m，1H)，2.19(s，3H)，3.56-3.64(m，1H)，3.67-3.77(m，1H)，4.31-4.40(m，1H)，7.68(dd，J＝9.5，9.5Hz，1H)，8.13(s，1H)，8.57(dd，J＝2.8，9.0Hz，1H)，8.87(d，J＝1.3Hz，1H)，11.46(s，1H)。MS(ES+)m/z 490.2[M+H]。Tert-butyl ((3aS,4R,8R)-4-(6-(3-chloro-5-cyanopicolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-65AB, 100 mg, 0.17 mmol) was dissolved in dichloromethane (10 mL) and trifluoroacetic acid (194 mg, 1.7 mmol) was added. The solution was stirred at room temperature for 4 h. The reaction mixture was basified with saturated aqueous sodium bicarbonate to pH = 7-8 and extracted with dichloromethane (2 x 10 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give the product. The product was repurified by preparative HPLC (C18, eluting with 0.05% ammonia in acetonitrile) and then lyophilized to afford the title compound as a white solid (18 mg, 22% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ1.51-1.67 (m, 1H), 1.79 (s, 3H), 2.07 (s, 3H), 2.17-2.26 (m, 1H), 2.19 (s, 3H), 3.56-3.64 (m, 1H), 3.67-3.77 (m, 1H) , 4.31-4.40 (m, 1H), 7.68 (dd, J=9.5, 9.5Hz, 1H), 8.13 (s, 1H), 8.57 (dd, J=2.8, 9.0Hz, 1H), 8.87 (d, J=1.3Hz, 1H), 11.46 (s, 1H). MS(ES+)m/z 490.2[M+H].

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺(25BA)N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide (25BA)

步骤1：((3aR，4R，8S)-4-(6-(3-氯-5-氰基吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-65BA)Step 1: tert-Butyl ((3aR,4R,8S)-4-(6-(3-chloro-5-cyanopicolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-65BA)

向((3aR，4R，8S)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39BA，150.0mg，0.35mmol)在THF(20mL)中的溶液中加入5-氰基-3-氯吡啶甲酸(96.5mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(222.2mg，1.75mmol)。将反应物在70℃搅拌16h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(2x10mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，用乙酸乙酯/石油醚1∶1洗脱)纯化，在真空中干燥后得到标题化合物，为黄色固体(100mg，50％产率)。¹H NMR(CDCl₃，400MHz)：δ0.84(s，3H)，1.54(s，9H)，1.73(2s，6H)，2.09-2.22(m，1H)，2.48-2.64(m，1H)，3.70-3.88(m，2H)，5.11-5.21(m，1H)，7.52-7.64(m，1H)，8.11-8.23(m，1H)，8.37-8.52(m，1H)，8.72-8.91(m，1H)，10.03-10.26(m，1H)，10.88-11.12(m，1H)。MS(ES+)m/z 590.2[M+H]。To a solution of tert-butyl ((3aR,4R,8S)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39BA, 150.0 mg, 0.35 mmol) in THF (20 mL) was added 5-cyano-3-chloropicolinic acid (96.5 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (222.2 mg, 1.75 mmol). The reaction was stirred at 70 ° C for 16 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (2×10 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by column chromatography (silica gel, eluted with ethyl acetate/petroleum ether 1:1) to give the title compound as a yellow solid (100 mg, 50% yield) after drying in vacuo. ¹ H NMR (CDCl ₃ , 400MHz): δ0.84 (s, 3H), 1.54 (s, 9H), 1.73 (2s, 6H), 2.09-2.22 (m, 1H), 2.48-2.64 (m, 1H), 3.70-3.88 (m, 2H), 5.11-5.21 (m, 1H), 7.52-7.64(m, 1H), 8.11-8.23(m, 1H), 8.37-8.52(m, 1H), 8.72-8.91(m, 1H), 10.03-10.26(m, 1H), 10.88-11.12(m, 1H). MS(ES+)m/z 590.2[M+H].

步骤2：N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺(25BA)Step 2: N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopicinamide (25BA)

将((3aR，4R，8S)-4-(6-(3-氯-5-氰基吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-65BA，100mg，0.17mmol)溶解在乙腈(20mL)中并且加入二溴化锌(115mg，0.51mmol)。然后，加入二氯甲烷(6mL)并且将得到的溶液在35℃搅拌18h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用二氯甲烷(2x20mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到产物。将产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)再纯化，然后冻干，得到标题化合物，为白色固体(16mg，19％产率)。¹H NMR(CDCl₃，400MHz)：δ0.96(s，3H)，1.83(s，3H)，1.84(s，3H)，2.17-2.29(m，1H)，2.59-2.69(m，1H)，3.73-3.86(m，2H)，5.10(dd，J＝7.1，11.2Hz，1H)，7.63(dd，J＝9.4，9.4Hz，1H)，8.20(d，J＝1.8Hz，1H)，8.49(dd，J＝2.9，8.9Hz，1H)，8.88(d，J＝1.5Hz，1H)，10.25(br s，1H)。MS(ES+)m/z 490.2[M+H]。Tert-butyl ((3aR,4R,8S)-4-(6-(3-chloro-5-cyanopicolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-65BA, 100 mg, 0.17 mmol) was dissolved in acetonitrile (20 mL) and zinc dibromide (115 mg, 0.51 mmol) was added. Then, dichloromethane (6 mL) was added and the resulting solution was stirred at 35 ° C for 18 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with dichloromethane (2x20 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give the product. The product was further purified by preparative HPLC (C18, eluting with 0.05% ammonia/acetonitrile) and then lyophilized to give the title compound as a white solid (16 mg, 19% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ0.96 (s, 3H), 1.83 (s, 3H), 1.84 (s, 3H), 2.17-2.29 (m, 1H), 2.59-2.69 (m, 1H), 3.73-3.86 (m, 2H), 5.10 (dd, J=7.1, 11.2Hz, 1H), 7.63 (dd, J=9.4, 9.4Hz, 1H), 8.20 (d, J=1.8Hz, 1H), 8.49 (dd, J=2.9, 8.9Hz, 1H), 8.88 (d, J=1.5Hz, 1H), 10.25 (br s, 1H). MS(ES+)m/z 490.2[M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡嗪-2-甲酰胺(26AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyrazine-2-carboxamide (26AB)

步骤1：((3aS，4R，8R)-4-(6-(5-(二氟甲氧基)吡嗪-2-甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-73AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(6-(5-(difluoromethoxy)pyrazine-2-carboxamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-73AB)

向((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，150mg，0.35mmol)在THF(20mL)中的溶液中加入5-(二氟甲氧基)吡嗪-2-甲酸(150mg，0.78mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(267mg，2.1mmol)。将反应物在70℃搅拌4h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(3x30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(硅胶，二氯甲烷/乙酸乙酯1∶1，UV)纯化，在真空中干燥后得到标题化合物，为黄色固体(0.1g，50％产率)。MS(ES+)m/z 598.2[M+H]。To a solution of tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-(difluoromethoxy)pyrazine-2-carboxylic acid (150 mg, 0.78 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 70 ° C for 4 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3×30 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated to afford the crude product. The crude product was purified by preparative TLC (silica gel, dichloromethane/ethyl acetate 1:1, UV) to afford the title compound as a yellow solid (0.1 g, 50% yield) after drying in vacuo. MS (ES+) m/z 598.2 [M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡嗪-2-甲酰胺(26AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyrazine-2-carboxamide (26AB)

将((3aS，4R，8R)-4-(6-(5-(二氟甲氧基)吡嗪-2-甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-73AB，100mg，0.18mmol)溶解在乙腈(20mL)和二氯甲烷(1mL)中。然后，加入二溴化锌(100mg，0.45mmol)并且将得到的溶液在40℃搅拌16h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用乙酸乙酯(3x30mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到产物。将产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)再纯化，然后冻干，得到标题化合物，为白色固体(32mg，38％产率)。¹H NMR(d6-DMSO，400MHz)：δ1.54-1.67(m，1H)，1.62(s，3H)，1.73(s，3H)，1.91(s，3H)，2.05-2.14(m，1H)，3.23-3.31(m，1H)，3.44-3.53(m，1H)，3.90-4.06(m，1H)，7.82(t，J＝71.3Hz，1H)，7.86-7.95(m，1H)，8.21-8.32(m，2H)，8.71-8.75(m，1H)，9.04(d，J＝1.3Hz，1H)，10.87(br s，1H)。MS(ES+)m/z498.2[M+H]。Tert-butyl ((3aS,4R,8R)-4-(6-(5-(difluoromethoxy)pyrazine-2-carboxamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-73AB, 100 mg, 0.18 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (1 mL). Zinc dibromide (100 mg, 0.45 mmol) was then added and the resulting solution was stirred at 40 °C for 16 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate (20 mL) and extracted with ethyl acetate (3x30 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give the product. The product was further purified by preparative HPLC (C18, eluting with 0.05% ammonia/acetonitrile) and then lyophilized to give the title compound as a white solid (32 mg, 38% yield). ¹ H NMR (d6-DMSO, 400MHz): δ1.54-1.67(m, 1H), 1.62(s, 3H), 1.73(s, 3H), 1.91(s, 3H), 2.05-2.14(m, 1H), 3.23-3.31(m, 1H), 3.44-3.53(m, 1H), 3.90-4.06 (m, 1H), 7.82 (t, J=71.3Hz, 1H), 7.86-7.95 (m, 1H), 8.21-8.32 (m, 2H), 8.71-8.75 (m, 1H), 9.04 (d, J=1.3Hz, 1H), 10.87 (br s, 1H). MS (ES+) m/z 498.2 [M+H].

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡嗪-2-甲酰胺(26BA)N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyrazine-2-carboxamide (26BA)

步骤1：((3aR，4R，8S)-4-(6-(5-(二氟甲氧基)吡嗪-2-甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-73BA)Step 1: tert-Butyl ((3aR,4R,8S)-4-(6-(5-(difluoromethoxy)pyrazine-2-carboxamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-73BA)

向((3aR，4R，8S)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39BA，150mg，0.35mmol)在THF(20mL)中的溶液中加入5-(二氟甲氧基)吡嗪-2-甲酸(150mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(267mg，2.1mmol)。将反应物在70℃搅拌4h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(3x30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(硅胶，二氯甲烷/乙酸乙酯1∶1，UV)纯化，在真空中干燥后得到标题化合物，为黄色固体(0.1g，50％产率)。¹H NMR(CDCl₃，400MHz)：δ0.86(s，3H)，1.54(s，9H)，1.70-1.77(m，6H)，2.06-2.22(m，1H)，2.58(ddd，J＝5.7，11.1，11.1Hz，1H)，3.70-3.89(m，2H)，5.17(dd，J＝7.2，11.2Hz，1H)，7.32-7.70(m，2H)，8.39(d，J＝1.3Hz，1H)，8.46(dd，J＝3.0，8.9Hz，1H)，9.03-9.10(m，1H)，10.01(s，1H)，11.04(br s，1H)。MS(ES+)m/z 598.2[M+H]。To a solution of tert-butyl ((3aR,4R,8S)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39BA, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-(difluoromethoxy)pyrazine-2-carboxylic acid (150 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 70 ° C for 4 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3×30 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated to give the crude product.The crude product was purified by preparative TLC (silica gel, dichloromethane/ethyl acetate 1:1, UV) to give the title compound as a yellow solid (0.1 g, 50% yield) after drying in vacuo. ¹ H NMR (CDCl ₃ , 400MHz): δ0.86 (s, 3H), 1.54 (s, 9H), 1.70-1.77 (m, 6H), 2.06-2.22 (m, 1H), 2.58 (ddd, J=5.7, 11.1, 11.1Hz, 1H), 3.70-3.89 (m, 2H), 5.17 (dd, J=7.2, 11.2Hz, 1H), 7.32-7.70 (m, 2H), 8.39 (d, J=1.3Hz, 1H), 8.46 (dd, J=3.0, 8.9Hz, 1H), 9.03-9.10 (m, 1H), 10.01 (s, 1H), 11.04 (br s, 1H). MS (ES+) m/z 598.2 [M+H].

步骤2：N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡嗪-2-甲酰胺(26BA)Step 2: N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyrazine-2-carboxamide (26BA)

将((3aR，4R，8S)-4-(6-(5-(二氟甲氧基)吡嗪-2-甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-73BA，100mg，0.18mmol)溶解在乙腈(20mL)和二氯甲烷(1mL)中。然后，加入二溴化锌(100mg，0.45mmol)并且将得到的溶液在40℃搅拌16h。将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用乙酸乙酯(3x30mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到标题化合物，为白色固体(33.0mg，38％产率)。¹H NMR(CDCl₃，400MHz)：δ1.01(s，3H)，1.88(2s，6H)，2.17-2.31(m，1H)，2.60-2.71(m，1H)，3.74-3.89(m，2H)，5.19(dd，J＝7.2，11.3Hz，1H)，7.52(t，J＝71.3Hz，1H)，7.66(dd，J＝9.4，9.4Hz，1H)，8.42(s，1H)，8.49-8.55(m，1H)，9.09(s，1H)，10.06(s，1H)。MS(ES+)m/z 498.3[M+H]。Tert-butyl ((3aR, 4R, 8S)-4-(6-(5-(difluoromethoxy)pyrazine-2-carboxamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-73BA, 100 mg, 0.18 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (1 mL). Zinc dibromide (100 mg, 0.45 mmol) was then added and the resulting solution was stirred at 40 ° C for 16 h. The reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3x30 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give the title compound as a white solid (33.0 mg, 38% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ1.01 (s, 3H), 1.88 (2s, 6H), 2.17-2.31 (m, 1H), 2.60-2.71 (m, 1H), 3.74-3.89 (m, 2H), 5.19 (dd, J=7.2, 11.3H z, 1H), 7.52 (t, J=71.3Hz, 1H), 7.66 (dd, J=9.4, 9.4Hz, 1H), 8.42 (s, 1H), 8.49-8.55 (m, 1H), 9.09 (s, 1H), 10.06 (s, 1H). MS(ES+)m/z 498.3[M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氟吡啶酰胺(27AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoropyridinecarboxamide (27AB)

步骤1：((3aS，4R，8R)-4-(3-氟-6-(5-氟吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-74AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(3-fluoro-6-(5-fluoropicolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-74AB)

向((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，150.0mg，0.35mmol)在THF(20mL)中的溶液中加入5-氟吡啶甲酸(80mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(267mg，2.1mmol)。将反应物在70℃搅拌4h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(3x30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(硅胶，二氯甲烷/乙酸乙酯1∶1，UV)纯化，在真空中干燥后得到标题化合物，为黄色固体(100mg，50％产率)。¹H NMR(CDCl₃，400MHz)：δ1.68(s，9H)，1.87(s，3H)，1.98(s，3H)，2.06-2.19(m，4H)，3.44-3.59(m，11H)，3.68(d，J＝13.2Hz，1H)，4.24-4.36(m，1H)，7.58-7.66(m，2H)，8.37(dd，J＝4.5，8.7Hz，1H)，8.45(d，J＝2.6Hz，1H)，8.51(dd，J＝3.0，8.9Hz，1H)，10.64(s，1H)，12.48-12.70(m，1H)。MS(ES+)m/z 549.1[M+H]。To a solution of tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 150.0 mg, 0.35 mmol) in THF (20 mL) was added 5-fluoropicolinic acid (80 mg, 0.53 mmol) followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 70 °C for 4 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3 x 30 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (silica gel, dichloromethane/ethyl acetate 1:1, UV) to afford the title compound after drying in vacuo as a yellow solid (100 mg, 50% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ1.68 (s, 9H), 1.87 (s, 3H), 1.98 (s, 3H), 2.06-2.19 (m, 4H), 3.44-3.59 (m, 11H), 3.68 (d, J=13.2Hz, 1H), 4.24-4.36 (m, 1H), 7.58-7.66 (m, 2H), 8.37 (dd, J=4.5, 8.7Hz, 1H), 8.45 (d, J=2.6Hz, 1H), 8.51 (dd, J=3.0, 8.9Hz, 1H), 10.64 (s, 1H), 12.48-12.70 (m, 1H). MS (ES+) m/z 549.1 [M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氟吡啶酰胺(27AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoropyridinecarboxamide (27AB)

将((3aS，4R，8R)-4-(3-氟-6-(5-氟吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-74AB，100mg，0.18mmol)溶解在乙腈(20mL)和二氯甲烷(1mL)中。加入二溴化锌(100mg，0.45mmol)并将溶液在40℃搅拌16h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用乙酸乙酯(3x30mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到黄色固体，为产物。将产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)再纯化，然后冻干，得到标题化合物，为白色固体(23mg，28％产率)。¹H NMR(CDCl₃，400MHz)：δ1.62-1.81(m，1H)，1.80(s，3H)，1.93(s，3H)，2.05-2.13(m，1H)，2.06(s，3H)，3.52(dd，J＝7.8，10.5Hz，1H)，3.71(ddd，J＝5.3，10.5，10.5Hz，1H)，4.24(ddd，J＝1.8，7.0，12.3Hz，1H)，7.59(dd，J＝9.0，10.3Hz，1H)，7.63(ddd，J＝2.8，8.3，8.3Hz，1H)，8.34(dd，J＝4.5，8.8Hz，1H)，8.48(dd，J＝3.2，8.9Hz，1H)，8.53(d，J＝2.5Hz，1H)，10.46(s，1H)。MS(ES+)m/z 449.1[M+H]。Tert-butyl ((3aS,4R,8R)-4-(3-fluoro-6-(5-fluoropicolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-74AB, 100 mg, 0.18 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (1 mL). Zinc dibromide (100 mg, 0.45 mmol) was added and the solution was stirred at 40 °C for 16 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3 x 30 mL). The combined extracts were dried over sodium sulfate, filtered, and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give a yellow solid as the product. The product was repurified by preparative HPLC (C18, eluting with 0.05% ammonia in acetonitrile) and then lyophilized to give the title compound as a white solid (23 mg, 28% yield). ¹ H NMR (CDCl ₃ , 400 MHz): δ 1.62-1.81 (m, 1H), 1.80 (s, 3H), 1.93 (s, 3H), 2.05-2.13 (m, 1H), 2.06 (s, 3H), 3.52 (dd, J=7.8, 10.5 Hz, 1H), 3.71 (ddd, J=5.3, 10.5, 10.5 Hz, 1H), 4.24 (ddd, J=1.8, 7 .0, 12.3Hz, 1H), 7.59 (dd, J=9.0, 10.3Hz, 1H), 7.63 (ddd, J=2.8, 8.3, 8.3Hz, 1H), 8.34 ( dd, J=4.5, 8.8Hz, 1H), 8.48 (dd, J=3.2, 8.9Hz, 1H), 8.53 (d, J=2.5Hz, 1H), 10.46 (s, 1H). MS(ES+)m/z 449.1[M+H].

N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氟吡啶酰胺(27BA)N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoropyridinecarboxamide (27BA)

步骤1：((3aR，4R，8S)-4-(3-氟-6-(5-氟吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-74BA)Step 1: tert-Butyl ((3aR,4R,8S)-4-(3-fluoro-6-(5-fluoropicolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-74BA)

向((3aR，4R，8S)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39BA，150mg，0.35mmol)在THF(20mL)中的溶液中加入5-氟吡啶甲酸(80mg，0.53mmol)，接着加入T3P(1.1g，1.75mmol，50％于乙酸乙酯中)和二异丙基乙胺(267mg，2.1mmol)。将反应物在70℃搅拌4h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释并且用乙酸乙酯(3x30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(硅胶，二氯甲烷/乙酸乙酯1∶1，UV)纯化，在真空中干燥后得到标题化合物，为黄色固体(100mg，50％产率)。¹H NMR(CDCl₃，400MHz)：δ0.75-0.93(m，3H)，1.49-1.63(m，9H)，1.70-1.83(m，6H)，2.06-2.23(m，1H)，2.52-2.66(m，1H)，3.74-3.91(m，2H)，5.23(dd，J＝7.3，11.2Hz，1H)，7.51-7.69(m，2H)，8.35(dd，J＝4.5，8.7Hz，1H)，8.44-8.58(m，2H)，10.35(s，1H)，11.05(s，1H)。MS(ES+)m/z 549.1[M+H]。To a solution of tert-butyl ((3aR,4R,8S)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39BA, 150 mg, 0.35 mmol) in THF (20 mL) was added 5-fluoropicolinic acid (80 mg, 0.53 mmol), followed by T3P (1.1 g, 1.75 mmol, 50% in ethyl acetate) and diisopropylethylamine (267 mg, 2.1 mmol). The reaction was stirred at 70 ° C for 4 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3×30 mL). The combined organic layers were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (silica gel, dichloromethane/ethyl acetate 1:1, UV) to afford the title compound after drying in vacuo as a yellow solid (100 mg, 50% yield). ¹ H NMR (CDCl ₃ , 400MHz): δ0.75-0.93 (m, 3H), 1.49-1.63 (m, 9H), 1.70-1.83 (m, 6H), 2.06-2.23 (m, 1H), 2.52-2.66 (m, 1H), 3.74-3.91 (m, 2H) , 5.23 (dd, J=7.3, 11.2Hz, 1H), 7.51-7.69 (m, 2H), 8.35 (dd, J=4.5, 8.7Hz, 1H), 8.44-8.58 (m, 2H), 10.35 (s, 1H), 11.05 (s, 1H). MS(ES+)m/z 549.1[M+H].

步骤2：N-(6-((3aR，4R，8S)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-5-氟吡啶酰胺(27BA)Step 2: N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoropyridinecarboxamide (27BA)

将((3aR，4R，8S)-4-(3-氟-6-(5-氟吡啶甲酰胺基)吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-74BA，100mg，0.18mmol)溶解在乙腈(20mL)和二氯甲烷(1mL)中。加入二溴化锌(100mg，0.45mmol)并且将溶液在40℃搅拌16h。之后，将反应混合物用饱和碳酸氢钠水溶液(20mL)稀释，用乙酸乙酯(3x30mL)萃取。将合并的萃取物经硫酸钠干燥，过滤并浓缩，得到粗产物。将粗产物通过制备型TLC(二氯甲烷/甲醇10∶1，UV)纯化，得到黄色固体，为产物。将产物通过制备型HPLC(C18，用0.05％氨/乙腈洗脱)再纯化，然后冻干，得到标题化合物，为白色固体(24mg，30％产率)。¹H NMR(CDCl₃，400MHz)：δ0.82(s，3H)，1.67(s，3H)，1.70(s，3H)，2.10-2.24(m，1H)，2.51-2.60(m，1H)，3.67-3.74(m，1H)，3.81(ddd，J＝4.8，10.5，10.5Hz，1H)，5.12(dd，J＝7.3，11.3Hz，1H)，7.51(dd，J＝9.0，10.3Hz，1H)，7.61(ddd，J＝2.8，8.2，8.2Hz，1H)，8.33(dd，J＝4.5，8.8Hz，1H)，8.40(dd，J＝2.9，8.9Hz，1H)，8.53(d，J＝2.5Hz，1H)，10.33(s，1H)。MS(ES+)m/z 449.1[M+H]。Tert-butyl ((3aR,4R,8S)-4-(3-fluoro-6-(5-fluoropicolinamido)pyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-74BA, 100 mg, 0.18 mmol) was dissolved in acetonitrile (20 mL) and dichloromethane (1 mL). Zinc dibromide (100 mg, 0.45 mmol) was added and the solution was stirred at 40 °C for 16 h. Afterwards, the reaction mixture was diluted with saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (3 x 30 mL). The combined extracts were dried over sodium sulfate, filtered and concentrated to give the crude product. The crude product was purified by preparative TLC (dichloromethane/methanol 10:1, UV) to give a yellow solid as the product. The product was repurified by preparative HPLC (C18, eluting with 0.05% ammonia in acetonitrile) and then lyophilized to give the title compound as a white solid (24 mg, 30% yield). ¹ H NMR (CDCl ₃ , 400 MHz): δ 0.82 (s, 3H), 1.67 (s, 3H), 1.70 (s, 3H), 2.10-2.24 (m, 1H), 2.51-2.60 (m, 1H), 3.67-3.74 (m, 1H), 3.81 (ddd, J=4.8, 10.5, 10.5 Hz, 1H), 5.12 (dd, J=7.3, 11.3 Hz, 1H). Hz, 1H), 7.51 (dd, J=9.0, 10.3Hz, 1H), 7.61 (ddd, J=2.8, 8.2, 8.2Hz, 1H), 8.33 (dd, J =4.5, 8.8Hz, 1H), 8.40 (dd, J=2.9, 8.9Hz, 1H), 8.53 (d, J=2.5Hz, 1H), 10.33 (s, 1H). MS(ES+)m/z 449.1[M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-(二氟甲氧基)吡啶酰胺(28AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-(difluoromethoxy)picolinamide (28AB)

步骤1：((3aS，4R，8R)-4-(6-(3-氯-5-(二氟甲氧基)吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-75AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(6-(3-chloro-5-(difluoromethoxy)picolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-75AB)

将3-氯-5-(二氟甲氧基)吡啶甲酸(78mg，353μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(62mg，494μmol)以及二甲基甲酰胺(0.242M于甲苯中，36μL，9μmol)。将混合物在室温搅拌2h。然后，将其在真空中浓缩(40℃，5毫巴)，得到3-氯-5-(二氟甲氧基)吡啶甲酰氯，为黄色油状物(85mg，定量的)。其后，将((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，100mg，235μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(60.7mg，82.1μL，470μmol)，接着加入3-氯-5-(二氟甲氧基)吡啶甲酰氯(见上，85mg，353μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌15min，接着在室温搅拌45min。然后，加入甲醇(5mL)，将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度20∶80至100∶0洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为浅橙色固体(90mg，61％产率)。HPLC(方法LCMS_fglm)t_R＝1.36min。MS(ES+)m/z 631.3[M+H]。3-Chloro-5-(difluoromethoxy)picolinic acid (78 mg, 353 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (62 mg, 494 μmol) and dimethylformamide (0.242 M in toluene, 36 μL, 9 μmol) were added. The mixture was stirred at room temperature for 2 h. It was then concentrated in vacuo (40° C., 5 mbar) to give 3-chloro-5-(difluoromethoxy)picolinic acid chloride as a yellow oil (85 mg, quantitative). Subsequently, tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 100 mg, 235 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (60.7 mg, 82.1 μL, 470 μmol) was added, followed by a solution of 3-chloro-5-(difluoromethoxy)picolinoyl chloride (see above, 85 mg, 353 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 15 min and then at room temperature for 45 min. Methanol (5 mL) was then added, and the mixture was stirred at room temperature for 15 min and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 20:80 to 100:0) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a light orange solid (90 mg, 61% yield). HPLC (Method LCMS_fglm) _tR = 1.36 min. MS (ES+) m/z 631.3 [M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3-氯-5-(二氟甲氧基)吡啶酰胺(28AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-(difluoromethoxy)picolinamide (28AB)

将((3aS，4R，8R)-4-(6-(3-氯-5-(二氟甲氧基)吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-75AB，90mg，143μmol)溶解在二氯甲烷(10mL)中并且加入三氟乙酸(268mg，181μL，2.35mmol)。将溶液在室温搅拌16h。之后，将混合物冷却至0-5℃(冰浴)，搅拌时小心加入水(20mL)和氨水(2M，8mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x50mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，24g，用甲醇中2M氨/二氯甲烷，梯度2∶98至6∶94洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为灰白色泡沫(70mg)。对映异构纯化通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)60/40，流速1.0mL/min)进行，得到所需其次洗脱的对映异构体，为灰白色粉末(48mg，62％)，以及相反的首先洗脱的对映异构体，为灰白色粉末(12mg，15％)。HPLC(方法LCMS梯度)t_R＝1.4min。¹H NMR(CDCl₃，300MHz)：δ1.59-1.76(m，1H)，1.91(s，3H)，2.01(s，3H)，2.10-2.20(m，1H)，2.14(s，3H)，3.55(dd，J＝7.7，10.7Hz，1H)，3.72(ddd，J＝5.1，10.7，10.7Hz，1H)，4.30(ddd，J＝1.9，7.2，12.2Hz，1H)，6.68(t，J＝71.3Hz，1H)，7.60(dd，J＝8.9，10.3Hz，1H)，7.69(d，J＝2.4Hz，1H)，8.11(br s，2H)，8.50(d，J＝2.4Hz，1H)，8.54(dd，J＝3.0，8.9Hz，1H)，11.21(s，1H)。MS(ES+)m/z 531.3[M+H]。Tert-butyl ((3aS,4R,8R)-4-(6-(3-chloro-5-(difluoromethoxy)picolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-75AB, 90 mg, 143 μmol) was dissolved in dichloromethane (10 mL) and trifluoroacetic acid (268 mg, 181 μL, 2.35 mmol) was added. The solution was stirred at room temperature for 16 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and water (20 mL) and aqueous ammonia (2 M, 8 mL) were carefully added with stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 50 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 24 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 2:98 to 6:94) and dried in vacuo (40° C., 5 mbar) to afford the title compound as an off-white foam (70 mg). Enantiomeric purification was performed by chiral preparative HPLC (Chiralpak AD, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.01% ammonium acetate) 60/40, flow rate 1.0 mL/min) to afford the desired second-eluting enantiomer as an off-white powder (48 mg, 62%) and the opposite first-eluting enantiomer as an off-white powder (12 mg, 15%). HPLC (method LCMS gradient) t _R = 1.4 min. ¹ H NMR (CDCl ₃ , 300MHz): δ1.59-1.76 (m, 1H), 1.91 (s, 3H), 2.01 (s, 3H), 2.10-2.20 (m, 1H), 2.14 (s, 3H), 3.55 (dd, J=7.7, 10.7Hz, 1H), 3.72 (ddd, J=5.1, 1 0.7, 10.7Hz, 1H), 4.30 (ddd, J=1.9, 7.2, 12.2Hz, 1H), 6.68 (t, J=71.3Hz, 1H), 7.60 (dd, J=8.9, 10.3Hz, 1H), 7.69 (d, J=2.4Hz, 1H), 8.11 (br s, 2H), 8.50 (d, J=2.4Hz, 1H), 8.54 (dd, J=3.0, 8.9Hz, 1H), 11.21 (s, 1H). MS(ES+)m/z 531.3[M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3，5-二氯吡啶酰胺(29AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridinamide (29AB)

步骤1：((3aS，4R，8R)-4-(6-(3，5-二氯吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-76AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(6-(3,5-dichloropicolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-76AB)

将3，5-二氯吡啶甲酸(68mg，353μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(62mg，494μmol)以及二甲基甲酰胺(0.242M于甲苯中，36μL，9μmol)。将混合物在室温搅拌2h。然后，将其在真空中浓缩(40℃，5毫巴)，得到3，5-二氯吡啶甲酰氯，为浅黄色油状物(74mg，定量的)。其后，将((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，100mg，235μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(61mg，82μL，470μmol)，接着加入3，5-二氯吡啶甲酰氯(见上，74mg，353μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌60min。然后，加入甲醇(5mL)，将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，24g，用乙酸乙酯/正庚烷，梯度20∶80至100∶0洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为浅紫色固体(75mg，53％产率)。HPLC(方法LCMS_fglm)t_R＝1.41min。MS(ES+)m/z599.2[M+H]。3,5-Dichloropicolinic acid (68 mg, 353 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5 ° C (ice bath) and oxalyl chloride (62 mg, 494 μmol) and dimethylformamide (0.242 M in toluene, 36 μL, 9 μmol) were added. The mixture was stirred at room temperature for 2 h. It was then concentrated in vacuo (40 ° C, 5 mbar) to give 3,5-dichloropicolinic acid chloride as a light yellow oil (74 mg, quantitative). Subsequently, tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 100 mg, 235 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (61 mg, 82 μL, 470 μmol) was added, followed by a solution of 3,5-dichloropicolinoyl chloride (see above, 74 mg, 353 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 60 min. Then, methanol (5 mL) was added, the mixture was stirred at room temperature for 15 min, and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 24 g, eluted with ethyl acetate/n-heptane, gradient 20:80 to 100:0) to afford the title compound as a pale purple solid (75 mg, 53% yield) after drying in vacuo (40° C., 5 mbar). HPLC (Method LCMS_fglm) _tR = 1.41 min. MS (ES+) m/z 599.2 [M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-3，5-二氯吡啶酰胺(29AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridinamide (29AB)

将((3aS，4R，8R)-4-(6-(3，5-二氯吡啶甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-76AB，75mg，125μmol)溶解在二氯甲烷(10mL)中并且加入三氟乙酸(268mg，181μL，2.35mmol)。将溶液在室温搅拌16h。之后，将混合物冷却至0-5℃(冰浴)，在搅拌时小心加入水(20mL)和氨水(2M，8mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x50mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度2∶98至6∶94洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为灰白色泡沫(67mg)。对映异构纯化通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)70/30，流速1.0mL/min)进行，得到所需其次洗脱的对映异构体，为灰白色粉末(49mg，79％)，和相反的首先洗脱的对映异构体，为灰白色粉末(12mg，19％)。HPLC(方法LCMS_梯度)t_R＝1.4min。¹H NMR(CDCl₃，300MHz)：δ1.59-1.78(m，1H)，1.89(s，3H)，1.99(s，3H)，2.08-2.19(m，1H)，2.12(s，3H)，3.54(dd，J＝7.8，10.6Hz，1H)，3.72(ddd，J＝5.0，10.6，10.6Hz，1H)，4.29(ddd，J＝1.9，7.2，12.2Hz，1H)，5.83(br s，2H)，7.60(dd，J＝9.0，10.2Hz，1H)，7.91(d，J＝2.0Hz，1H)，8.52(dd，J＝2.9，9.0Hz，1H)，8.56(d，J＝2.0Hz，1H)，11.00(s，1H)。MS(ES+)m/z 499.2[M+H]。Tert-butyl ((3aS,4R,8R)-4-(6-(3,5-dichloropicolinamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-76AB, 75 mg, 125 μmol) was dissolved in dichloromethane (10 mL) and trifluoroacetic acid (268 mg, 181 μL, 2.35 mmol) was added. The solution was stirred at room temperature for 16 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and water (20 mL) and aqueous ammonia (2 M, 8 mL) were carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 50 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 2:98 to 6:94) and dried in vacuo (40° C., 5 mbar) to give the title compound as an off-white foam (67 mg). Enantiomeric purification was performed by chiral preparative HPLC (Chiralpak AD, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.01% ammonium acetate) 70/30, flow rate 1.0 mL/min) to give the desired second-eluting enantiomer as an off-white powder (49 mg, 79%) and the opposite first-eluting enantiomer as an off-white powder (12 mg, 19%). HPLC (method LCMS_gradient) t _R = 1.4 min. ¹ H NMR (CDCl ₃ , 300MHz): δ1.59-1.78 (m, 1H), 1.89 (s, 3H), 1.99 (s, 3H), 2.08-2.19 (m, 1H), 2.12 (s, 3H), 3.54 (dd, J =7.8, 10.6Hz, 1H), 3.72 (ddd, J = 5.0, 10.6, 10.6Hz, 1H), 4.29 (ddd, J = 1.9, 7.2, 12.2Hz, 1H), 5.83 (br s, 2H), 7.60 (dd, J=9.0, 10.2Hz, 1H), 7.91 (d, J=2.0Hz, 1H), 8.52 (dd, J=2.9, 9.0Hz, 1H), 8.56 (d, J=2.0Hz, 1H), 11.00 (s, 1H). MS(ES+)m/z 499.2[M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-4-氰基苯甲酰胺(30AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-4-cyanobenzamide (30AB)

步骤1：((3aS，4R，8R)-4-(6-(4-氰基苯甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-77AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(6-(4-cyanobenzamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-77AB)

向((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，100mg，0.23mmol)在二氯甲烷(2.3mL)中的溶液中加入4-氰基苯甲酸(86.4mg，0.59mmol)，接着加入T3P(0.70mL，1.2mmol，50％于乙酸乙酯中)和二异丙基乙胺(0.25mL，1.4mmol)。将反应物在密封瓶中在60℃搅拌20h。之后，通过加入氨水溶液(25％m/m，0.2mL)终止反应，并在室温搅拌1h。将混合物用碳酸氢钠水溶液(1M，20mL)稀释并且用二氯甲烷(2x30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并在真空中浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度20∶80至100∶0洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为黄色固体(0.033g，25％产率)。MS(ES+)m/z 555[M+H]。To a solution of tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 100 mg, 0.23 mmol) in dichloromethane (2.3 mL) was added 4-cyanobenzoic acid (86.4 mg, 0.59 mmol), followed by T3P (0.70 mL, 1.2 mmol, 50% in ethyl acetate) and diisopropylethylamine (0.25 mL, 1.4 mmol). The reaction was stirred at 60 ° C. in a sealed bottle for 20 h. Afterwards, the reaction was quenched by the addition of aqueous ammonia solution (25% m / m, 0.2 mL) and stirred at room temperature for 1 h. The mixture was diluted with aqueous sodium bicarbonate (1 M, 20 mL) and extracted with dichloromethane (2 x 30 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo to afford the crude product. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 20: 80 to 100: 0) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a yellow solid (0.033 g, 25% yield). MS (ES+) m/z 555 [M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-4-氰基苯甲酰胺(30AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-4-cyanobenzamide (30AB)

在室温向((3aS，4R，8R)-4-(6-(4-氰基苯甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-77AB，0.033g，0.060mmol)在二氯甲烷(0.6mL)中的溶液中加入三氟乙酸(0.092mL，1.19mmol)。将混合物在室温搅拌15h。然后，将其在真空中浓缩并且将残留物通过制备型HPLC(C18，用水/乙腈/三乙胺洗脱)纯化，得到标题化合物，为灰白色固体(3mg，11％产率)。¹H NMR(CDCl₃，300MHz)：δ1.55-1.73(m，1H)，1.99(s，3H)，2.09(s，3H)，2.15-2.28(m，1H)，2.20(s，3H)，3.55-3.65(m，1H)，3.67-3.79(m，1H)，4.34-4.44(m，1H)，7.66(dd，J＝9.2，10.2Hz，1H)，7.77(d，J＝8.5Hz，2H)，8.23(d，J＝8.3Hz，2H)，8.57(dd，J＝3.2，9.1Hz，1H)，11.10(s，1H)。MS(ES+)m/z 455[M+H]。To a solution of ((3aS, 4R, 8R)-4-(6-(4-cyanobenzamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamic acid tert-butyl ester (Int-77AB, 0.033 g, 0.060 mmol) in dichloromethane (0.6 mL) was added trifluoroacetic acid (0.092 mL, 1.19 mmol) at room temperature. The mixture was stirred at room temperature for 15 h. The product was then concentrated in a vacuum and the residue was purified by preparative HPLC (C18, eluted with water/acetonitrile/triethylamine) to give the title compound as an off-white solid (3 mg, 11% yield). ¹ H NMR (CDCl ₃ , 300MHz): δ1.55-1.73 (m, 1H), 1.99 (s, 3H), 2.09 (s, 3H), 2.15-2.28 (m, 1H), 2.20 (s, 3H), 3.55-3.65 (m, 1H), 3.67-3.79 (m, 1H), 4.34 -4.44 (m, 1H), 7.66 (dd, J=9.2, 10.2Hz, 1H), 7.77 (d, J=8.5Hz, 2H), 8.23 (d, J=8.3Hz, 2H), 8.57 (dd, J=3.2, 9.1Hz, 1H), 11.10 (s, 1H). MS(ES+)m/z 455[M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-2-氯-4-氰基苯甲酰胺(31AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-2-chloro-4-cyanobenzamide (31AB)

步骤1：((3aS，4R，8R)-4-(6-(2-氯-4-氰基苯甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-78AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(6-(2-chloro-4-cyanobenzamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-78AB)

向((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，100mg，0.23mmol)在四氢呋喃(2.4mL)中的溶液中加入2-氯-4-氰基苯甲酸(107mg，0.59mmol)，接着加入T3P(0.70mL，1.2mmol，50％于乙酸乙酯中)和二异丙基乙胺(0.25mL，1.4mmol)。将反应物在密封管中在60℃搅拌20h。之后，通过加入氨水溶液(25％m/m，0.2mL)终止反应，并且在室温搅拌1h。将混合物用碳酸氢钠水溶液(1M，20mL)稀释并且用二氯甲烷(2x30mL)萃取。将合并的有机层经硫酸钠干燥，过滤并在真空中浓缩，得到粗产物。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度20∶80至100∶0洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为黄色固体(0.035g，25％产率)。MS(ES+)m/z 589[M+H]。To a solution of tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 100 mg, 0.23 mmol) in tetrahydrofuran (2.4 mL) was added 2-chloro-4-cyanobenzoic acid (107 mg, 0.59 mmol), followed by T3P (0.70 mL, 1.2 mmol, 50% in ethyl acetate) and diisopropylethylamine (0.25 mL, 1.4 mmol). The reaction was stirred at 60 ° C. in a sealed tube for 20 h. Afterwards, the reaction was quenched by the addition of aqueous ammonia solution (25% m / m, 0.2 mL) and stirred at room temperature for 1 h. The mixture was diluted with aqueous sodium bicarbonate (1 M, 20 mL) and extracted with dichloromethane (2 x 30 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo to afford the crude product. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 20: 80 to 100: 0) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a yellow solid (0.035 g, 25% yield). MS (ES+) m/z 589 [M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-2-氯-4-氰基苯甲酰胺(31AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-2-chloro-4-cyanobenzamide (31AB)

在室温向((3aS，4R，8R)-4-(6-(2-氯-4-氰基苯甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-78AB，0.035g，0.059mmol)在二氯甲烷(0.6mL)中的溶液中加入三氟乙酸(0.092mL，1.19mmol)。将混合物在室温搅拌15h。然后，将其在真空中浓缩并且将残留物通过制备型HPLC(C18，用水/乙腈/三乙胺洗脱)纯化，得到标题化合物，为灰白色固体(9mg，31％产率)。¹H NMR(CDCl₃，300MHz)：δ1.52-1.70(m，1H)，1.94(s，3H)，2.05(s，3H)，2.17-2.29(m，1H)，2.19(s，3H)，3.60(dd，J＝7.7，10.9Hz，1H)，3.74(ddd，J＝5.0，10.7，10.7Hz，1H)，4.40(ddd，J＝1.5，7.2，11.8Hz，1H)，7.60-7.71(m，3H)，7.74-7.77(m，1H)，8.58(dd，J＝3.2，9.0Hz，1H)，10.78(s，1H)，11.17(s，1H)，11.88(br s，1H)。MS(ES+)m/z 489[M+H]。To a solution of tert-butyl ((3aS,4R,8R)-4-(6-(2-chloro-4-cyanobenzamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-78AB, 0.035 g, 0.059 mmol) in dichloromethane (0.6 mL) was added trifluoroacetic acid (0.092 mL, 1.19 mmol) at room temperature. The mixture was stirred at room temperature for 15 h. It was then concentrated in vacuo and the residue was purified by preparative HPLC (C18, eluting with water/acetonitrile/triethylamine) to give the title compound as an off-white solid (9 mg, 31% yield). ¹ H NMR (CDCl ₃ , 300MHz): δ1.52-1.70 (m, 1H), 1.94 (s, 3H), 2.05 (s, 3H), 2.17-2.29 (m, 1H ), 2.19 (s, 3H), 3.60 (dd, J=7.7, 10.9Hz, 1H), 3.74 (ddd, J=5.0, 10.7, 10.7 Hz, 1H), 4.40 (ddd, J=1.5, 7.2, 11.8Hz, 1H), 7.60-7.71 (m, 3H), 7.74-7.77 (m, 1H), 8.58 (dd, J=3.2, 9.0Hz, 1H), 10.78 (s, 1H), 11.17 (s, 1H), 11.88 (br s, 1H). MS (ES+) m/z 489 [M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(32AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (32AA)

步骤1：((4aR，5R，9R)-5-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-79AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-79AA)

将5-氰基-3-甲基吡啶甲酸(195mg，1.2mmol)悬浮在二氯甲烷(4mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(203mg，140μL，1.6mmol)以及一滴二甲基甲酰胺和甲苯(1∶3，v/v)的混合物。将混合物在室温搅拌1.75h。然后，将其在真空中浓缩，将残留物用正庚烷(3mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-氰基-3-甲基吡啶甲酰氯，为红色油状物(220mg，99％)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AA，70mg，147μmol)溶解在二氯甲烷(3mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(37.7mg，50μL，292μmol)，接着加入5-氰基-3-甲基吡啶甲酰氯(见上，35mg，194μmol)在二氯甲烷(650μL)中的溶液。将反应混合物在0-5℃搅拌1.5h。然后，加入乙醇(100μL)，将混合物在室温搅拌30min，并且将混合物倒入饱和碳酸氢钠水溶液(10mL)中并且用二氯甲烷(1x40mL，2x20mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，80g，用乙酸乙酯/正庚烷，梯度20∶80至40∶60洗脱)纯化，在真空中干燥(50℃，5毫巴)后得到标题化合物，为白色固体(90mg，99％)。HPLC(方法LCMS_梯度)t_R＝3.4min。¹H NMR(CDCl₃，400MHz)：δ1.49(s，9H)，1.81(s，3H)，1.83(s，3H)，1.95(s，3H)，2.52-2.82(m，2H)，2.87(s，3H)，3.64-3.88(m，2H)，4.56-4.62(m，1H)，7.62(dd，J＝9.0，10.6Hz，1H)，7.97-7.99(m，1H)，8.45(dd，J＝3.1，9.0Hz，1H)，8.79-8.81(m，1H)，10.50(s，1H)，11.36(s，1H)。MS(ES+)m/z 620.2[M+H]。5-cyano-3-methylpicolinic acid (195mg, 1.2mmol) is suspended in dichloromethane (4mL), the suspension is cooled to 0-5 ℃ (ice bath) and a mixture of oxalyl chloride (203mg, 140 μ L, 1.6mmol) and a drop of dimethylformamide and toluene (1: 3, v/v) is added. The mixture is stirred at room temperature for 1.75h. Then, it is concentrated in a vacuum, the residue is processed with n-heptane (3mL) and concentrated again and dried in a vacuum (40 ℃, 5 mbar) to obtain 5-cyano-3-methylpicolinic acid chloride, which is a red oil (220mg, 99%). Thereafter, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AA, 70 mg, 147 μmol) was dissolved in dichloromethane (3 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (37.7 mg, 50 μL, 292 μmol) was added, followed by a solution of 5-cyano-3-methylpicolinyl chloride (see above, 35 mg, 194 μmol) in dichloromethane (650 μL). The reaction mixture was stirred at 0-5° C. for 1.5 h. The product of 4-nitro- 4- pyridine-2-yl _pyridine -3 ... ¹ H NMR (CDCl ₃ , 400MHz): δ1.49 (s, 9H), 1.81 (s, 3H), 1.83 (s, 3H), 1.95 (s, 3H), 2.52-2.82 (m, 2H), 2.87 (s, 3H), 3.64-3.88 (m, 2H), 4.56-4.62 (m, 1H), 7.62 (dd, J=9.0, 10.6Hz, 1H), 7.97-7.99 (m, 1H), 8.45 (dd, J=3.1, 9.0Hz, 1H), 8.79-8.81 (m, 1H), 10.50 (s, 1H), 11.36 (s, 1H). MS(ES+)m/z 620.2[M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(32AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (32AA)

将((4aR，5R，9R)-5-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-79AA，90mg，145μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(1.33g，900μL，11.7mmol)。将溶液在室温搅拌0.5h。之后，将其在真空中浓缩(25℃，5毫巴)。将残留物棕色粘性油状物溶解在二氯甲烷(40mL)中，加入饱和碳酸氢钠水溶液(15mL)。搅拌5min后，分离相并且将水相用二氯甲烷(2x15mL)萃取。将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物溶解在二氯甲烷(1mL)中，加入MTBE(5mL)并且再次在真空中浓缩，在真空中干燥(50℃，5毫巴)后得到灰白色固体，为粗产物。将粗产物通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)70/30，流速1.0mL/min)纯化，得到所需(-)-旋光对映异构体，为白色固体(49.8mg，66％)，以及相反(+)-旋光对映异构体，为白色固体(24.2mg，32％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和叔丁基甲醚(3mL)并浓缩并在真空中在50℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.7min。¹H NMR(CDCl₃，400MHz)：δ1.89(s，3H)，1.91(s，3H)，1.96(s，3H)，2.22-2.39(m，1H)，2.57-2.79(m，1H)，2.87(s，3H)，3.57-3.85(m，2H)，4.07-4.15(m，1H)，7.09(br s，2H，exch)，7.56(dd，J＝9.0，10.8Hz，1H)，7.97-8.00(m，1H)，8.43(dd，J＝3.0，8.9Hz，1H)，8.79(d，J＝1.6Hz，1H)，10.46(s，1H)。MS(ES+)m/z 520.1[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-79AA, 90 mg, 145 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (1.33 g, 900 μL, 11.7 mmol) was added. The solution was stirred at room temperature for 0.5 h. Afterwards, it was concentrated in vacuo (25° C., 5 mbar). The residue, a brown viscous oil, was dissolved in dichloromethane (40 mL) and saturated aqueous sodium bicarbonate (15 mL) was added. The product of 4-nitro-1-oxo-2-oxo-4-oxo-1-oxo-4-oxo-2 ... For transfer purposes the material was dissolved in dichloromethane (1 mL) and tert-butyl methyl ether (3 mL) and concentrated and dried in vacuo at 50° C./5 mbar. HPLC (method LCMS_gradient) t _R =1.7 min. ¹ H NMR (CDCl ₃ , 400MHz): δ1.89 (s, 3H), 1.91 (s, 3H), 1.96 (s, 3H), 2.22-2.39 (m, 1H), 2.5 7-2.79(m, 1H), 2.87(s, 3H), 3.57-3.85(m, 2H), 4.07-4.15(m, 1H), 7.09(br s, 2H, exch), 7.56 (dd, J=9.0, 10.8Hz, 1H), 7.97-8.00 (m, 1H), 8.43 (dd, J=3.0, 8.9Hz, 1H), 8.79 (d, J=1.6Hz, 1H), 10.46 (s, 1H). MS (ES+) m/z 520.1 [M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氟吡啶酰胺(33AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoropyridinecarboxamide (33AA)

步骤1：((4aR，5R，9R)-3，3-二氟-5-(3-氟-6-(5-氟吡啶甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-80AA)Step 1: tert-Butyl ((4aR,5R,9R)-3,3-difluoro-5-(3-fluoro-6-(5-fluoropicolinamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-80AA)

将5-氟吡啶甲酸(47.6mg，337μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(56.9mg，39.3μL，449μmol)以及一滴二甲基甲酰胺和甲苯(1∶3，v/v)的混合物。将混合物在室温搅拌2h。加入第二部分草酰氯(28.7mg，19.8μL，226μmol)并且将混合物在室温搅拌另外1h以驱使反应完成。然后，将其在真空中浓缩，将残留物用甲苯(5mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-氟吡啶甲酰氯，为红色油状物(52.1mg，97％)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AA，115mg，242μmol)溶解在二氯甲烷(6mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(61.9mg，83.6μL，479μmol)，接着加入5-氟吡啶甲酰氯(见上，52.1mg，326μmol)在二氯甲烷(5mL)中的溶液。将反应混合物在0-5℃搅拌15min，接着在室温搅拌14h。然后，加入甲醇(2mL)，将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度20∶80至35∶65洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(100mg，69％)。HPLC(方法LCMS_梯度)t_R＝3.4min。MS(ES+)m/z 599.2[M+H]。5-Fluoropicolinic acid (47.6mg, 337μmol) is suspended in dichloromethane (3mL), suspension is cooled to 0-5 ℃ (ice bath) and add oxalyl chloride (56.9mg, 39.3μL, 449μmol) and a drop of dimethylformamide and toluene (1: 3, v/v) mixture.Mixture is stirred at room temperature for 2h.Add second portion oxalyl chloride (28.7mg, 19.8μL, 226μmol) and mixture is stirred at room temperature for other 1h to drive reaction to complete.Then, it is concentrated in a vacuum, residue is handled and concentrated again and dried in a vacuum (40 ℃, 5 mbar) with toluene (5mL), obtain 5-fluoropicolinic acid chloride, be red oily matter (52.1mg, 97%). Subsequently, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AA, 115 mg, 242 μmol) was dissolved in dichloromethane (6 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (61.9 mg, 83.6 μL, 479 μmol) was added, followed by a solution of 5-fluoropicolinyl chloride (see above, 52.1 mg, 326 μmol) in dichloromethane (5 mL). The reaction mixture was stirred at 0-5° C. for 15 min and then at room temperature for 14 h. Methanol (2 mL) was then added, and the mixture was stirred at room temperature for 15 min and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 20:80 to 35:65) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a white solid (100 mg, 69%). HPLC (method LCMS_Gradient) t _R = 3.4 min. MS (ES+) m/z 599.2 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氟吡啶酰胺(33AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoropyridinecarboxamide (33AA)

将((4aR，5R，9R)-3，3-二氟-5-(3-氟-6-(5-氟吡啶甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-80AA，99mg，165μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(378mg，254μL，3.30mmol)。将溶液在室温搅拌4h。之后，将混合物冷却至0-5℃(冰浴)，并且在搅拌时小心加入氨水(8％m/m，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x5mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，20g，用甲醇中2M氨/二氯甲烷，梯度0∶100至10∶90洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色泡沫(80mg，96％)。对映异构纯化通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)80/20，流速1.0mL/min)进行，得到所需(-)-旋光的对映异构体，为白色固体(51mg，61％)，以及相反(+)-旋光的对映异构体，为白色固体(18.3mg，22％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和叔丁基甲醚(3mL)中并浓缩并在真空中在40℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.7min。¹H NMR(CDCl₃，300MHz)：δ1.80(s，3H)，1.85(2s，6H)，2.29-2.44(m，1H)，2.57-2.79(m，1H)，3.58-3.88(m，2H)，4.16-4.27(m，1H)，7.52(dd，J＝9.0，10.8Hz，1H)，7.63(ddd，J＝2.6，8.3，8.3Hz，1H)，8.31-8.46(m，2H)，8.54(d，J＝2.6Hz，1H)，10.26(s，1H)。MS(ES+)m/z 499.1[M+H]。Tert-butyl ((4aR,5R,9R)-3,3-difluoro-5-(3-fluoro-6-(5-fluoropicolinamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-80AA, 99 mg, 165 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (378 mg, 254 μL, 3.30 mmol) was added. The solution was stirred at room temperature for 4 h. Afterwards, the mixture was cooled to 0-5 ° C (ice bath) and aqueous ammonia (8% m/m, 6 mL) was carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2x5 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 20 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 0:100 to 10:90) and dried in vacuo (40°C, 5 mbar) to give the title compound as a white foam (80 mg, 96%). Enantiomeric purification was performed by chiral preparative HPLC (Chiralpak AD, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.01% ammonium acetate) 80/20, flow rate 1.0 mL/min) to give the desired (-)-optically active enantiomer as a white solid (51 mg, 61%), and the opposite (+)-optically active enantiomer as a white solid (18.3 mg, 22%). For transfer purposes the material was dissolved in dichloromethane (1 mL) and tert-butyl methyl ether (3 mL) and concentrated and dried in vacuo at 40° C./5 mbar. HPLC (method LCMS_gradient) t _R =1.7 min. ¹ H NMR (CDCl ₃ , 300MHz): δ1.80 (s, 3H), 1.85 (2s, 6H), 2.29-2.44 (m, 1H), 2.57-2.79 (m, 1H), 3.58-3.88 (m, 2H), 4.16-4.27 (m, 1H), 7. 52 (dd, J=9.0, 10.8Hz, 1H), 7.63 (ddd, J=2.6, 8.3, 8.3Hz, 1H), 8.31-8.46 (m, 2H), 8.54 (d, J=2.6Hz, 1H), 10.26 (s, 1H). MS(ES+)m/z 499.1[M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基吡啶酰胺(34AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyanopyridineamide (34AA)

步骤1：((4aR，5R，9R)-5-(6-(5-氰基吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-81AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(5-cyanopicolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-81AA)

将5-氰基吡啶甲酸(60mg，405μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(86.1mg，59.4μL，679μmol)以及一滴二甲基甲酰胺和甲苯(1∶3，v/v)的混合物。将混合物在室温搅拌18h。然后，将其在真空中浓缩，将残留物用甲苯(5mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-氰基吡啶甲酰氯，为红色油状物(52mg，77％)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AA，110mg，231μmol)溶解在二氯甲烷(6mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(59.2mg，80μL，458μmol)，接着加入5-氟吡啶甲酰氯(见上，52mg，312μmol)在二氯甲烷(5mL)中的溶液。将反应混合物在0-5℃搅拌45min。然后，加入甲醇(2mL)，将混合物在室温搅拌15min，并且将混合物在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度20∶80至40∶60洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(140mg，96％)。HPLC(方法LCMS_梯度)t_R＝3.2min。¹H NMR(CDCl₃，300MHz)：δ1.50(s，9H)，1.82(s，3H)，1.84(s，3H)，1.97(s，3H)，2.52-2.85(m，2H)，3.64-3.92(m，2H)，4.54-4.65(m，1H)，7.66(dd，J＝9.0，10.6Hz，1H)，8.25(dd，J＝2.0，8.3Hz，1H)，8.46(dd，J＝0.7，8.2Hz，1H)，8.50(dd，J＝3.1，9.0Hz，1H)，8.97-9.00(m，1H)，10.33(s，1H)，11.38(s，1H)。MS(ES+)m/z 606.2[M+H]。5-Cyanopicolinic acid (60 mg, 405 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (86.1 mg, 59.4 μL, 679 μmol) and a drop of dimethylformamide and toluene (1: 3, v/v) were added. The mixture was stirred at room temperature for 18 h. The mixture was then concentrated in vacuo, the residue was treated with toluene (5 mL) and concentrated again and dried in vacuo (40° C., 5 mbar) to give 5-cyanopicolinic acid chloride as a red oil (52 mg, 77%). Afterwards, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AA, 110 mg, 231 μmol) was dissolved in dichloromethane (6 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (59.2 mg, 80 μL, 458 μmol) was added, followed by a solution of 5-fluoropicolinyl chloride (see above, 52 mg, 312 μmol) in dichloromethane (5 mL). The reaction mixture was stirred at 0-5° C. for 45 min. Then, methanol (2 mL) was added, the mixture was stirred at room temperature for 15 min, and the mixture was concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 20: 80 to 40: 60) and the title compound was obtained after drying in vacuo (40° C., 5 mbar) as a white solid (140 mg, 96%). HPLC (method LCMS-gradient) t _R =3.2 min. ¹ H NMR (CDCl ₃ , 300MHz): δ1.50 (s, 9H), 1.82 (s, 3H), 1.84 (s, 3H), 1.97 (s, 3H), 2.52- 2.85 (m, 2H), 3.64-3.92 (m, 2H), 4.54-4.65 (m, 1H), 7.66 (dd, J=9.0, 10. 6Hz, 1H), 8.25 (dd, J=2.0, 8.3Hz, 1H), 8.46 (dd, J=0.7, 8.2Hz, 1H), 8.50 (dd, J=3.1, 9.0Hz, 1H), 8.97-9.00 (m, 1H), 10.33 (s, 1H), 11.38 (s, 1H). MS (ES+) m/z 606.2 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氰基吡啶酰胺(34AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyanopyridineamide (34AA)

将((4aR，5R，9R)-5-(6-(5-氰基吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-81AA，137mg，226μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(516mg，349μL，4.52mmol)。将溶液在室温搅拌90min。之后，将混合物冷却至0-5℃(冰浴)，并且在搅拌时小心加入氨水(8％m/m，8mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x5mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，20g，用甲醇中2M氨/二氯甲烷，梯度0∶100至10∶90洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(100mg，94％)。对映异构纯化通过手性制备型HPLC(ChiralpakAD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)70/30，流速1.0mL/min)进行，得到所需(-)-旋光的对映异构体，为白色固体(68mg，59％)，以及相反(+)-旋光的对映异构体，为白色固体(15mg，13％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和叔丁基甲醚(3mL)中并浓缩并在真空中在40℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.7min。¹H NMR(CDCl₃，300MHz)：δ1.83(s，3H)，1.88(s，3H)，1.89(s，3H)，2.26-2.41(m，1H)，2.57-2.80(m，1H)，3.58-3.78(m，2H)，4.11-4.21(m，1H)，5.55(br s，2H，exch)，7.56(dd，J＝8.9，10.9Hz，1H)，8.24(dd，J＝2.0，8.1Hz，1H)，8.40-8.48(m，2H)，8.95-9.00(m，1H)，10.29(s，1H)。MS(ES+)m/z 506.1[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-(5-cyanopicolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-81AA, 137 mg, 226 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (516 mg, 349 μL, 4.52 mmol) was added. The solution was stirred at room temperature for 90 min. Afterwards, the mixture was cooled to 0-5°C (ice bath) and aqueous ammonia (8% m/m, 8 mL) was carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2x5 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 20 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 0: 100 to 10: 90) and dried in vacuo (40° C., 5 mbar) to give the title compound as a white solid (100 mg, 94%). Enantiomeric purification was performed by chiral preparative HPLC (Chiralpak AD, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.01% ammonium acetate) 70/30, flow rate 1.0 mL/min) to give the desired (-)-optically active enantiomer as a white solid (68 mg, 59%), and the opposite (+)-optically active enantiomer as a white solid (15 mg, 13%). For transfer purposes the material was dissolved in dichloromethane (1 mL) and tert-butyl methyl ether (3 mL) and concentrated and dried in vacuo at 40° C./5 mbar. HPLC (method LCMS_gradient) t _R =1.7 min. ¹ H NMR (CDCl ₃ , 300MHz): δ1.83 (s, 3H), 1.88 (s, 3H), 1.89 (s, 3H), 2.26-2.41 (m, 1H), 2.57-2.80 (m, 1H), 3.58-3.78 (m, 2H), 4.11-4.21 (m, 1H), 5.55 (br s, 2H, exch), 7.56 (dd, J=8.9, 10.9Hz, 1H), 8.24 (dd, J=2.0, 8.1Hz, 1H), 8.40-8.48 (m, 2H), 8.95-9.00 (m, 1H), 10.29 (s, 1H). MS(ES+)m/z 506.1[M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺(35AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide (35AA)

步骤1：((4aR，5R，9R)-5-(6-(5-(二氟甲氧基)吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-82AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(5-(difluoromethoxy)picolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-82AA)

将5-(二氟甲氧基)吡啶甲酸(76.6mg，405μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(68.4mg，47.2μL，539μmol)以及二甲基甲酰胺(0.25M于甲苯中，40.5μL，10μmol)。将混合物在室温搅拌17h。然后，将其在真空中浓缩，将残留物用甲苯(5mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-(二氟甲氧基)吡啶甲酰氯，为绿色油状物(72mg，86％)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AA，110mg，231μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(59.8mg，80.8μL，463μmol)，接着加入5-(二氟甲氧基)吡啶甲酰氯(见上，72mg，347μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌45min。然后，加入甲醇(5mL)，并将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，20g，用乙酸乙酯/正庚烷，梯度5∶95至70∶30洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为蜡状固体(173mg)，在不进一步纯化的情况下将其用于下一步骤。HPLC(方法LCMS_fglm)t_R＝1.50min。MS(ES+)m/z 647.5[M+H]。5-(Difluoromethoxy)picolinic acid (76.6 mg, 405 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5 ° C (ice bath) and oxalyl chloride (68.4 mg, 47.2 μL, 539 μmol) and dimethylformamide (0.25 M in toluene, 40.5 μL, 10 μmol) were added. The mixture was stirred at room temperature for 17 h. It was then concentrated in vacuo, the residue was treated with toluene (5 mL) and concentrated again and dried in vacuo (40 ° C, 5 mbar) to give 5-(difluoromethoxy)picolinoyl chloride as a green oil (72 mg, 86%). Afterwards, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AA, 110 mg, 231 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (59.8 mg, 80.8 μL, 463 μmol) was added, followed by a solution of 5-(difluoromethoxy)picolinoyl chloride (see above, 72 mg, 347 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 45 min. Methanol (5 mL) was then added, and the mixture was stirred at room temperature for 15 min and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 20 g, eluted with ethyl acetate/n-heptane, gradient 5:95 to 70:30) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a waxy solid (173 mg), which was used in the next step without further purification. HPLC (Method LCMS_fglm) _tR = 1.50 min. MS (ES+) m/z 647.5 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(二氟甲氧基)吡啶酰胺(35AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)picolinamide (35AA)

将((4aR，5R，9R)-5-(6-(5-(二氟甲氧基)吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-82AA，173mg，参见前一步骤，231μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(528mg，356μL，4.63mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)，并且在搅拌时小心加入氨水(8％m/m，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x5mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度0∶100至10∶90洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(121mg)。对映异构纯化通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)80/20，流速1.0mL/min)进行，得到所需(-)-旋光的首先洗脱的对映异构体，为白色固体(66mg，经2个步骤52％)，以及相反(+)-旋光的对映异构体，为白色固体(22mg，17％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和叔丁基甲醚(3mL)中并浓缩并在真空中在40℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.8min。¹H NMR(CDCl₃，300MHz)：δ1.86(s，3H)，1.90(2s，6H)，2.27-2.42(m，1H)，2.56-2.80(m，1H)，3.58-3.86(m，2H)，4.11-4.21(m，1H)，5.41(br s，2H，exch)，6.67(t，J＝71.8Hz，1H)，7.54(dd，J＝9.1，10.7Hz，1H)，7.70(dd，J＝2.5，8.6Hz，1H)，8.34(d，J＝8.7Hz，1H)，8.43(dd，J＝3.0，8.9Hz，1H)，8.55(d，J＝2.4Hz，1H)，10.29(s，1H)。MS(ES+)m/z 547.1[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-(5-(difluoromethoxy)picolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-82AA, 173 mg, see previous step, 231 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (528 mg, 356 μL, 4.63 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and aqueous ammonia (8% m/m, 6 mL) was carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 5 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 0:100 to 10:90) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a white solid (121 mg). Enantiomeric purification was performed by chiral preparative HPLC (Chiralpak AD, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.01% ammonium acetate) 80/20, flow rate 1.0 mL/min) to afford the desired (-)-optically active first-eluting enantiomer as a white solid (66 mg, 52% over 2 steps), and the opposite (+)-optically active enantiomer as a white solid (22 mg, 17%). For transfer purposes, the material was dissolved in dichloromethane (1 mL) and tert-butyl methyl ether (3 mL) and concentrated and dried in vacuo at 40° C./5 mbar. HPLC (method LCMS_gradient) t _R =1.8 min. ¹ H NMR (CDCl ₃ , 300 MHz): δ 1.86 (s, 3H), 1.90 (2s, 6H), 2.27-2.42 (m, 1H), 2.56-2.80 (m, 1H), 3.58-3.86 (m, 2H), 4.11-4.21 (m, 1H), 5.41 (br s, 2H, exch), 6.67 (t, J=71.8Hz, 1H), 7.54 (dd, J=9.1, 10.7Hz, 1H), 7.70 (dd, J=2.5, 8.6Hz, 1 H), 8.34 (d, J=8.7Hz, 1H), 8.43 (dd, J=3.0, 8.9Hz, 1H), 8.55 (d, J=2.4Hz, 1H), 10.29 (s, 1H). MS(ES+)m/z 547.1[M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺(36AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide (36AA)

步骤1：((4aR，5R，9R)-3，3-二氟-5-(3-氟-6-(5-(2，2，3，3-四氟丙氧基)吡啶甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-83AA)Step 1: tert-Butyl ((4aR,5R,9R)-3,3-difluoro-5-(3-fluoro-6-(5-(2,2,3,3-tetrafluoropropoxy)picolinamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-83AA)

将5-(2，2，3，3-四氟丙氧基)吡啶甲酸(103mg，405μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(68.4mg，47.2μL，539μmol)以及二甲基甲酰胺(0.25M于甲苯中，40.5μL，10μmol)。将混合物在室温搅拌15h。然后，将其在真空中浓缩，将残留物用甲苯(5mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-(2，2，3，3-四氟丙氧基)吡啶甲酰氯，为橙色油状物(94.2mg，86％)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AA，110mg，231μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(59.8mg，80.8μL，463μmol)，接着加入5-(2，2，3，3-四氟丙氧基)吡啶甲酰氯(见上，94.2mg，347μmol)在二氯甲烷(3mL)中的溶液中。将反应混合物在0-5℃搅拌15min，接着在室温搅拌90min。然后，加入甲醇(5mL)，将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，20g，用乙酸乙酯/正庚烷，梯度5∶95至70∶30洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色泡沫(173mg)，在不进一步纯化的情况下将其用于下一步骤。HPLC(方法LCMS_fglm)t_R＝1.53min。MS(ES+)m/z 711.3[M+H]。5-(2,2,3,3-tetrafluoropropoxy)picolinic acid (103 mg, 405 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (68.4 mg, 47.2 μL, 539 μmol) and dimethylformamide (0.25 M in toluene, 40.5 μL, 10 μmol) were added. The mixture was stirred at room temperature for 15 h. It was then concentrated in vacuo, the residue was treated with toluene (5 mL) and concentrated again and dried in vacuo (40° C., 5 mbar) to give 5-(2,2,3,3-tetrafluoropropoxy)picolinic acid chloride as an orange oil (94.2 mg, 86%). Afterwards, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AA, 110 mg, 231 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (59.8 mg, 80.8 μL, 463 μmol) was added followed by a solution of 5-(2,2,3,3-tetrafluoropropoxy)picolinoyl chloride (see above, 94.2 mg, 347 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5°C for 15 min, followed by 90 min at room temperature. Methanol (5 mL) was then added, the mixture was stirred at room temperature for 15 min, and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 20 g, eluted with ethyl acetate/n-heptane, gradient 5:95 to 70:30) and dried in vacuo (40°C, 5 mbar) to give the title compound as a white foam (173 mg), which was used in the next step without further purification. HPLC (Method LCMS_fglm) _tR = 1.53 min. MS (ES+) m/z 711.3 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(2，2，3，3-四氟丙氧基)吡啶酰胺(36AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)picolinamide (36AA)

将((4aR，5R，9R)-3，3-二氟-5-(3-氟-6-(5-(2，2，3，3-四氟丙氧基)吡啶甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-83AA，173mg，参见前一步骤，231μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(528mg，356μL，4.63mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)，并且在搅拌时小心加入氨水(8％m/m，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x5mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度0∶100至10∶90洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(127mg)。对映异构纯化通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)80/20，流速1.0mL/min)进行，得到所需(-)-旋光的首先洗脱的对映异构体，为白色固体(85mg，经2个步骤60％)，以及相反(+)-旋光的对映异构体，为白色固体(26mg，18％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和叔丁基甲醚(3mL)中并浓缩并在真空中在40℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝2.1min。¹H NMR(CDCl₃，300MHz)：δ1.82(s，3H)，1.87(2s，6H)，2.28-2.44(m，1H)，2.56-2.80(m，1H)，3.57-3.88(m，2H)，4.02(br s，2H)，4.14-4.25(m，1H)，4.52(t，J＝11.8Hz，1H)，6.08(tt，J＝4.2，53.1Hz，1H)，7.44(dd，J＝2.8，8.7Hz，1H)，7.52(dd，J＝9.0，10.8Hz，1H)，8.30(d，J＝8.7Hz，1H)，8.37-8.44(m，2H)，10.26(s，1H)。MS(ES+)m/z 611.1[M+H]。Tert-butyl ((4aR,5R,9R)-3,3-difluoro-5-(3-fluoro-6-(5-(2,2,3,3-tetrafluoropropoxy)picolinamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-83AA, 173 mg, see previous step, 231 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (528 mg, 356 μL, 4.63 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and aqueous ammonia (8% m/m, 6 mL) was carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 5 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 0:100 to 10:90) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a white solid (127 mg). Enantiomeric purification was performed by chiral preparative HPLC (Chiralpak AD, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.01% ammonium acetate) 80/20, flow rate 1.0 mL/min) to afford the desired (-)-optically active first-eluting enantiomer as a white solid (85 mg, 60% over 2 steps), and the opposite (+)-optically active enantiomer as a white solid (26 mg, 18%). For transfer purposes, the material was dissolved in dichloromethane (1 mL) and tert-butyl methyl ether (3 mL) and concentrated and dried in vacuo at 40° C./5 mbar. HPLC (method LCMS_gradient) t _R = 2.1 min. ¹ H NMR (CDCl ₃ , 300 MHz): δ 1.82 (s, 3H), 1.87 (2s, 6H), 2.28-2.44 (m, 1H), 2.56-2.80 (m, 1H), 3.57-3.88 (m, 2H), 4.02 (br s, 2H), 4.14-4.25 (m, 1H), 4.52 (t, J=11.8Hz, 1H), 6.08 (tt, J=4.2, 53.1Hz, 1H), 7.44 (dd, J=2.8, 8.7Hz, 1H), 7.52 (dd, J=9.0, 10.8Hz, 1H), 8.30 (d, J=8.7Hz, 1H), 8.37-8.44 (m, 2H), 10.26 (s, 1H). MS(ES+)m/z 611.1[M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺(37AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide (37AA)

步骤1：((4aR，5R，9R)-5-(6-(3-氯-5-氰基吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-84AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(3-chloro-5-cyanopicolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-84AA)

将3-氯-5-氰基吡啶甲酸(73.9mg，405μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(68.4mg，47.2μL，539μmol)以及二甲基甲酰胺(0.25M于甲苯中，40.5μL，10μmol)。将混合物在室温搅拌17h。然后，将其在真空中浓缩，将残留物用甲苯(5mL)并且再次浓缩并在真空中干燥(40℃，5毫巴)处理，得到3-氯-5-氰基吡啶甲酰氯，为棕色油状物(69.7mg，86％)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AA，110mg，231μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(59.8mg，80.8μL，463μmol)，接着加入3-氯-5-氰基吡啶甲酰氯(见上，69.7mg，347μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌15min，接着在室温搅拌90min。然后，加入甲醇(5mL)，将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度10∶90至60∶40洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(141mg，95％)。HPLC(方法LCMS_fglm)t_R＝1.44min。MS(ES+)m/z 640.1[M+H]。3-Chloro-5-cyanopicolinic acid (73.9 mg, 405 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5 ° C (ice bath) and oxalyl chloride (68.4 mg, 47.2 μL, 539 μmol) and dimethylformamide (0.25 M in toluene, 40.5 μL, 10 μmol) were added. The mixture was stirred at room temperature for 17 h. It was then concentrated in vacuo, and the residue was treated with toluene (5 mL) and concentrated again and dried in vacuo (40 ° C, 5 mbar) to give 3-chloro-5-cyanopicolinic acid chloride as a brown oil (69.7 mg, 86%). Subsequently, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AA, 110 mg, 231 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (59.8 mg, 80.8 μL, 463 μmol) was added, followed by a solution of 3-chloro-5-cyanopicolinyl chloride (see above, 69.7 mg, 347 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 15 min and then at room temperature for 90 min. Methanol (5 mL) was then added, and the mixture was stirred at room temperature for 15 min and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 10:90 to 60:40) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a white solid (141 mg, 95%). HPLC (Method LCMS_fglm) _tR = 1.44 min. MS (ES+) m/z 640.1 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺(37AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopicinamide (37AA)

将((4aR，5R，9R)-5-(6-(3-氯-5-氰基吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-84AA，141mg，220μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(528mg，356μL，4.63mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)，并且在搅拌时小心加入氨水(8％m/m，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x5mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度0∶100至10∶90洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(110mg)。对映异构纯化通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)70/30，流速1.0mL/min)进行，得到所需(-)-旋光的首先洗脱的对映异构体，为白色固体(66mg，56％)，以及相反(+)-旋光的对映异构体，为白色固体(22mg，19％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和叔丁基甲醚(3mL)中并浓缩并在真空中在40℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.7min。¹H NMR(CDCl₃，300MHz)：δ1.83(s，3H)，1.86(s，3H)，1.87(s，3H)，2.27-2.42(m，1H)，2.56-2.79(m，1H)，3.58-3.87(m，2H)，4.12-4.22(m，1H)，4.67(br s，2H)，7.55(dd，J＝8.9，10.7Hz，1H)，8.21(d，J＝1.6Hz，1H)，8.41(dd，J＝3.0，8.9Hz，1H)，8.86(d，J＝1.8Hz，1H)，10.15(s，1H)。MS(ES+)m/z 540.1[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-(3-chloro-5-cyanopicolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-84AA, 141 mg, 220 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (528 mg, 356 μL, 4.63 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and aqueous ammonia (8% m/m, 6 mL) was carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 5 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 0:100 to 10:90) and dried in vacuo (40° C., 5 mbar) to give the title compound as a white solid (110 mg). Enantiomeric purification was performed by chiral preparative HPLC (Chiralpak AD, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.01% ammonium acetate) 70/30, flow rate 1.0 mL/min) to give the desired (-)-optically active enantiomer, the first eluting enantiomer, as a white solid (66 mg, 56%), and the opposite (+)-optically active enantiomer, as a white solid (22 mg, 19%). For transfer purposes the material was dissolved in dichloromethane (1 mL) and tert-butyl methyl ether (3 mL) and concentrated and dried in vacuo at 40° C./5 mbar. HPLC (method LCMS_gradient) t _R =1.7 min. ¹ H NMR (CDCl ₃ , 300MHz): δ1.83 (s, 3H), 1.86 (s, 3H), 1.87 (s, 3H), 2.27-2.42 (m, 1H), 2.56-2.79 (m, 1H), 3.58-3.87 (m, 2H), 4.12-4.22 (m, 1H), 4.67 (br s, 2H), 7.55 (dd, J=8.9, 10.7Hz, 1H), 8.21 (d, J=1.6Hz, 1H), 8.41 (dd, J=3.0, 8.9Hz, 1H), 8.86 (d, J=1.8Hz, 1H), 10.15 (s, 1H). MS(ES+)m/z 540.1[M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺(38AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide (38AA)

步骤1：((4aR，5R，9R)-3，3-二氟-5-(3-氟-6-(5-甲氧基吡嗪-2-甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-85AA)Step 1: tert-Butyl ((4aR,5R,9R)-3,3-difluoro-5-(3-fluoro-6-(5-methoxypyrazine-2-carboxamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-85AA)

将5-甲氧基吡嗪-2-甲酸(62.4mg，405μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(68.4mg，47.2μL，539μmol)以及二甲基甲酰胺(0.25M于甲苯中，40.5μL，10μmol)。将混合物在室温搅拌17h。然后，将其在真空中浓缩，将残留物用甲苯(5mL)处理并且再次浓缩并在真空中干燥(40℃，5毫巴)，得到5-甲氧基吡嗪-2-碳酰氯，为棕色油状物(59.9mg，86％)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AA，110mg，231μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(59.8mg，80.8μL，463μmol)，接着加入5-甲氧基吡嗪-2-碳酰氯(见上，59.9mg，347μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌15min，接着在室温搅拌90min。然后，加入甲醇(5mL)，将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度10∶90至70∶30洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色泡沫(131mg，93％)。HPLC(方法LCMS_fglm)t_R＝1.49min。MS(ES+)m/z 612.2[M+H]。5-Methoxypyrazine-2-carboxylic acid (62.4 mg, 405 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (68.4 mg, 47.2 μL, 539 μmol) and dimethylformamide (0.25 M in toluene, 40.5 μL, 10 μmol) were added. The mixture was stirred at room temperature for 17 h. It was then concentrated in vacuo, the residue was treated with toluene (5 mL) and concentrated again and dried in vacuo (40° C., 5 mbar) to give 5-methoxypyrazine-2-carbonyl chloride as a brown oil (59.9 mg, 86%). Subsequently, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AA, 110 mg, 231 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (59.8 mg, 80.8 μL, 463 μmol) was added, followed by a solution of 5-methoxypyrazine-2-carbonyl chloride (see above, 59.9 mg, 347 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 15 min and then at room temperature for 90 min. Methanol (5 mL) was then added, and the mixture was stirred at room temperature for 15 min and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 10:90 to 70:30) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a white foam (131 mg, 93%). HPLC (Method LCMS_fglm) _tR = 1.49 min. MS (ES+) m/z 612.2 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-甲氧基吡嗪-2-甲酰胺(38AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide (38AA)

将((4aR，5R，9R)-3，3-二氟-5-(3-氟-6-(5-甲氧基吡嗪-2-甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-85AA，131mg，214μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(528mg，356μL，4.63mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)，并且在搅拌时小心加入氨水(8％m/m，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x 5mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度0∶100至10∶90洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(110mg)。对映异构纯化通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)80/20，流速1.0mL/min)进行，得到所需(-)-旋光的首先洗脱的对映异构体，为白色固体(75mg，68％)，以及相反(+)-旋光的对映异构体，为白色固体(20mg，18％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和叔丁基甲醚(3mL)中并浓缩并在真空中在50℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.6min。¹H NMR(CDCl₃，300MHz)：δ1.80(s，3H)，1.85(2s，6H)，2.28-2.43(m，1H)，2.55-2.79(m，1H)，3.57-3.87(m，2H)，4.09(s，3H)，4.11(br s，2H)，4.15-4.25(m，1H)，7.51(dd，J＝9.1，10.9Hz，1H)，8.23(d，J＝1.2Hz，1H)，8.40(dd，J＝3.0，8.9Hz，1H)，9.04(d，J＝1.2Hz，1H)，9.97(s，1H)。MS(ES+)m/z 512.2[M+H]。Tert-butyl ((4aR,5R,9R)-3,3-difluoro-5-(3-fluoro-6-(5-methoxypyrazine-2-carboxamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-85AA, 131 mg, 214 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (528 mg, 356 μL, 4.63 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and aqueous ammonia (8% m/m, 6 mL) was carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 5 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 0:100 to 10:90) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a white solid (110 mg). Enantiomeric purification was performed by chiral preparative HPLC (Chiralpak AD, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.01% ammonium acetate) 80/20, flow rate 1.0 mL/min) to afford the desired (-)-optically active first-eluting enantiomer as a white solid (75 mg, 68%), and the opposite (+)-optically active enantiomer as a white solid (20 mg, 18%). For transfer purposes the material was dissolved in dichloromethane (1 mL) and tert-butyl methyl ether (3 mL) and concentrated and dried in vacuo at 50° C./5 mbar. HPLC (method LCMS_gradient) t _R =1.6 min. ¹ H NMR (CDCl ₃ , 300MHz): δ 1.80 (s, 3H), 1.85 (2s, 6H), 2.28-2.43 (m, 1H), 2.55-2.79 (m, 1H), 3.57-3.87 (m, 2H), 4.09 (s, 3H), 4.11 (br s, 2H), 4.15-4.25 (m, 1H), 7.51 (dd, J=9.1, 10.9Hz, 1H), 8.23 (d, J=1.2Hz, 1H), 8.40 (dd, J=3.0, 8.9Hz, 1H), 9.04 (d, J=1.2Hz, 1H), 9.97 (s, 1H). MS(ES+)m/z 512.2[M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺(39AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide (39AA)

步骤1：((4aR，5R，9R)-3，3-二氟-5-(3-氟-6-(5-氟-3-甲基吡啶甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-86AA)Step 1: tert-Butyl ((4aR,5R,9R)-3,3-difluoro-5-(3-fluoro-6-(5-fluoro-3-methylpicolinamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-86AA)

将5-氟-3-甲基吡啶甲酸(57.4mg，370μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(62.5mg，43.1μL，492μmol)以及二甲基甲酰胺(0.25M于甲苯中，37μL，9.2μmol)。将混合物在室温搅拌2h。然后，将其在真空中浓缩(40℃，5毫巴)，得到5-氟-3-甲基吡啶甲酰氯，为棕色油状物(50.4mg，78％)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AA，92mg，193μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(50mg，67.6μL，387μmol)，接着加入5-氟-3-甲基吡啶甲酰氯(见上，50.4mg，290μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌15min，接着在室温搅拌90min。然后，加入甲醇(5mL)，将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度10∶90至40∶60洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(118mg，定量的)。HPLC(方法LCMS_fglm)t_R＝1.56min。MS(ES+)m/z 613.2[M+H]。5-Fluoro-3-methylpicolinic acid (57.4 mg, 370 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (62.5 mg, 43.1 μL, 492 μmol) and dimethylformamide (0.25 M in toluene, 37 μL, 9.2 μmol) were added. The mixture was stirred at room temperature for 2 h. It was then concentrated in vacuo (40° C., 5 mbar) to give 5-fluoro-3-methylpicolinic acid chloride as a brown oil (50.4 mg, 78%). Subsequently, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AA, 92 mg, 193 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (50 mg, 67.6 μL, 387 μmol) was added, followed by a solution of 5-fluoro-3-methylpicolinyl chloride (see above, 50.4 mg, 290 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 15 min and then at room temperature for 90 min. Methanol (5 mL) was then added, and the mixture was stirred at room temperature for 15 min and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 10:90 to 40:60) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a white solid (118 mg, quantitative). HPLC (Method LCMS_fglm) _tR = 1.56 min. MS (ES+) m/z 613.2 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氟-3-甲基吡啶酰胺(39AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpicolinamide (39AA)

将((4aR，5R，9R)-3，3-二氟-5-(3-氟-6-(5-氟-3-甲基吡啶甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-86AA，118mg，193μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(441mg，298μL，3.87mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)，并且在搅拌时小心加入氨水(8％m/m，6mL)，直到水层的pH是11-12。相分离后，将水层用乙酸乙酯(2x 50mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度0∶100至10∶90洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(60mg)。对映异构纯化通过手性制备型HPLC(Reprosil Chiral NR，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)80/20，流速1.0mL/min)进行，得到所需(-)-旋光的首先洗脱的对映异构体，为白色固体(32mg，32％)，以及相反(+)-旋光的对映异构体，为白色固体(7mg，7％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和叔丁基甲醚(3mL)中并浓缩并在真空中在40℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.7min。¹H NMR(CDCl₃，300MHz)：δ1.80(s，3H)，1.86(2s，6H)，2.29-2.44(m，1H)，2.56-2.78(m，1H)，2.84(s，3H)，3.58-3.87(m，2H)，4.15-4.24(m，1H)，4.42(br s，2H)，7.36-7.43(m，1H)，7.50(dd，J＝9.1，10.9Hz，1H)，8.34-8.41(m，2H)，10.43(s，1H)。MS(ES+)m/z 513.2[M+H]。Tert-butyl ((4aR,5R,9R)-3,3-difluoro-5-(3-fluoro-6-(5-fluoro-3-methylpicolinamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-86AA, 118 mg, 193 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (441 mg, 298 μL, 3.87 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and aqueous ammonia (8% m/m, 6 mL) was carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with ethyl acetate (2 x 50 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 0:100 to 10:90) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a white solid (60 mg). Enantiomeric purification was performed by chiral preparative HPLC (Reprosil Chiral NR, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.01% ammonium acetate) 80/20, flow rate 1.0 mL/min) to afford the desired (-)-optically active first-eluting enantiomer as a white solid (32 mg, 32%), and the opposite (+)-optically active enantiomer as a white solid (7 mg, 7%). For transfer purposes the material was dissolved in dichloromethane (1 mL) and tert-butyl methyl ether (3 mL) and concentrated and dried in vacuo at 40° C./5 mbar. HPLC (method LCMS_gradient) t _R =1.7 min. ¹ H NMR (CDCl ₃ , 300MHz): δ1.80 (s, 3H), 1.86 (2s, 6H), 2.29-2.44 (m, 1H), 2.56-2.78 (m, 1H), 2.84 (s, 3H), 3.58-3.87 (m, 2H), 4.15-4.24 (m, 1H), 4.42 (br s, 2H), 7.36-7.43 (m, 1H), 7.50 (dd, J=9.1, 10.9Hz, 1H), 8.34-8.41 (m, 2H), 10.43 (s, 1H). MS(ES+)m/z 513.2[M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3，5-二氯吡啶酰胺(40AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridinecarboxamide (40AA)

步骤1：((4aR，5R，9R)-5-(6-(3，5-二氯吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-87AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(3,5-dichloropicolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-87AA)

将3，5-二氯吡啶甲酸(46.1mg，240μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(42.6mg，29.4μL，336μmol)以及二甲基甲酰胺(0.137M于甲苯中，43.8μL，6μmol)。将混合物在室温搅拌17h。然后，将其在真空中浓缩(40℃，5毫巴)，得到3，5-二氯吡啶甲酰氯，为黄色油状物(50.6mg，定量的)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AAp，80mg，168μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(41.3mg，55.8μL，320μmol)，接着3，5-二氯吡啶甲酰氯(见上，50.6mg，240μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌15min，接着在室温搅拌90min。然后，加入甲醇(5mL)，并且将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度10∶90至40∶60洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为灰白色固体(115mg)，在不进一步纯化的情况下将其用于下一步骤。HPLC(方法LCMS_fglm)t_R＝1.56min。MS(ES+)m/z 649.1[M+H]。3,5-Dichloropicolinic acid (46.1 mg, 240 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5°C (ice bath) and oxalyl chloride (42.6 mg, 29.4 μL, 336 μmol) and dimethylformamide (0.137 M in toluene, 43.8 μL, 6 μmol) were added. The mixture was stirred at room temperature for 17 h. It was then concentrated in vacuo (40°C, 5 mbar) to give 3,5-dichloropicolinic acid chloride as a yellow oil (50.6 mg, quantitative). Subsequently, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AAp, 80 mg, 168 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (41.3 mg, 55.8 μL, 320 μmol) was added, followed by a solution of 3,5-dichloropicolinoyl chloride (see above, 50.6 mg, 240 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 15 min and then at room temperature for 90 min. Methanol (5 mL) was then added, and the mixture was stirred at room temperature for 15 min and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 10:90 to 40:60) and dried in vacuo (40° C., 5 mbar) to give the title compound as an off-white solid (115 mg), which was used in the next step without further purification. HPLC (Method LCMS_fglm) _tR = 1.56 min. MS (ES+) m/z 649.1 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3，5-二氯吡啶酰胺(40AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridinecarboxamide (40AA)

将((4aR，5R，9R)-5-(6-(3，5-二氯吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-87AA，115mg，参见前一步骤，168μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(384mg，259μL，3.36mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)，并且在搅拌时小心加入氨水(8％m/m，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x 5mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度0∶100至10∶90洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色粉末(85mg，经2个步骤92％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和叔丁基甲醚(3mL)中并浓缩并在真空中在40℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.7min。¹H NMR(CDCl₃，300MHz)：δ1.78(s，3H)，1.83(2s，6H)，2.30-2.47(m，1H)，2.54-2.79(m，1H)，3.57-3.88(m，2H)，4.17-4.30(m，1H)，4.41(br s，2H)，7.50(dd，J＝9.3，10.3Hz，1H)，7.94(s，1H)，8.34-8.43(m，1H)，8.57(d，J＝1.0Hz，1H)，10.17(s，1H)。MS(ES+)m/z 549.0[M+H]。Tert-butyl ((4aR, 5R, 9R)-5-(6-(3,5-dichloropicolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-87AA, 115 mg, see previous step, 168 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (384 mg, 259 μL, 3.36 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and aqueous ammonia (8% m/m, 6 mL) was carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 5 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 0: 100 to 10: 90) and dried in vacuo (40° C., 5 mbar) to give the title compound as a white powder (85 mg, 92% over 2 steps). For transfer purposes, the material was dissolved in dichloromethane (1 mL) and tert-butyl methyl ether (3 mL) and concentrated and dried in vacuo at 40° C./5 mbar. HPLC (method LCMS_gradient) t _R = 1.7 min. ¹ H NMR (CDCl ₃ , 300MHz): δ 1.78 (s, 3H), 1.83 (2s, 6H), 2.30-2.47 (m, 1H), 2.54-2.79 (m, 1H), 3.57-3.88 (m, 2H), 4.17-4.30 (m, 1H), 4.41 (br s, 2H), 7.50 (dd, J=9.3, 10.3Hz, 1H), 7.94 (s, 1H), 8.34-8.43 (m, 1H), 8.57 (d, J=1.0Hz, 1H), 10.17 (s, 1H). MS(ES+)m/z 549.0[M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺(41AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide (41AA)

步骤1：((4aR，5R，9R)-5-(6-(3-氯-5-氟吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-88AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(3-chloro-5-fluoropicolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-88AA)

将3-氯-5-氟吡啶甲酸(42.1mg，240μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(42.6mg，29.4μL，336μmol)以及二甲基甲酰胺(0.137M于甲苯中，43.8μL，6μmol)。将混合物在室温搅拌17h。然后，将其在真空中浓缩(40℃，5毫巴)，得到3-氯-5-氟吡啶甲酰氯，为棕色油状物(49mg)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AAp，80mg，168μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(41.3mg，55.8μL，320μmol)，接着加入3-氯-5-氟吡啶甲酰氯(见上，49mg，240μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌15min，接着在室温搅拌90min。然后，加入甲醇(5mL)，将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度10∶90至40∶60洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为灰白色固体(110mg)，在不进一步纯化的情况下将其用于下一步骤。HPLC(方法LCMS_梯度)t_R＝3.4min。MS(ES+)m/z 633.1[M+H]。3-Chloro-5-fluoropicolinic acid (42.1 mg, 240 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (42.6 mg, 29.4 μL, 336 μmol) and dimethylformamide (0.137 M in toluene, 43.8 μL, 6 μmol) were added. The mixture was stirred at room temperature for 17 h. It was then concentrated in vacuo (40° C., 5 mbar) to give 3-chloro-5-fluoropicolinic acid chloride as a brown oil (49 mg). Subsequently, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AAp, 80 mg, 168 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (41.3 mg, 55.8 μL, 320 μmol) was added, followed by a solution of 3-chloro-5-fluoropicolinyl chloride (see above, 49 mg, 240 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 15 min and then at room temperature for 90 min. Methanol (5 mL) was then added, the mixture was stirred at room temperature for 15 min, and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 10: 90 to 40: 60) and dried in vacuo (40° C., 5 mbar) to give the title compound as an off-white solid (110 mg), which was used in the next step without further purification. HPLC (Method LCMS_Gradient) t _R = 3.4 min. MS (ES+) m/z 633.1 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3-氯-5-氟吡啶酰胺(41AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinecarboxamide (41AA)

将((4aR，5R，9R)-5-(6-(3-氯-5-氟吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-88AA，110mg，参见前一步骤，168μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(384mg，259μL，3.36mmol)。将溶液在室温搅拌2h。之后，将混合物冷却至0-5℃(冰浴)，并且在搅拌时小心加入氨水(8％m/m，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x 5mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度0∶100至10∶90洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为灰白色粉末(85mg，经2个步骤95％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和叔丁基甲醚(3mL)中并浓缩并在真空中在40℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.5min。¹H NMR(CDCl₃，300MHz)：δ1.78(s，3H)，1.83(2s，6H)，2.31-2.46(m，1H)，2.55-2.78(m，1H)，3.58-3.88(m，2H)，4.18-4.29(m，1H)，4.41(br s，2H)，7.50(dd，J＝8.9，10.9Hz，1H)，7.68(dd，J＝2.4，7.9Hz，1H)，8.38(dd，J＝3.0，8.9Hz，1H)，8.50(d，J＝1.8Hz，1H)，10.15(s，1H)。MS(ES+)m/z 533.1[M+H]。Tert-butyl ((4aR, 5R, 9R)-5-(6-(3-chloro-5-fluoropicolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-88AA, 110 mg, see previous step, 168 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (384 mg, 259 μL, 3.36 mmol) was added. The solution was stirred at room temperature for 2 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and aqueous ammonia (8% m/m, 6 mL) was carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 5 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 0: 100 to 10: 90) and dried in vacuo (40° C., 5 mbar) to give the title compound as an off-white powder (85 mg, 95% over 2 steps). For transfer purposes, the material was dissolved in dichloromethane (1 mL) and tert-butyl methyl ether (3 mL) and concentrated and dried in vacuo at 40° C./5 mbar. HPLC (method LCMS_gradient) t _R = 1.5 min. ¹ H NMR (CDCl ₃ , 300MHz): δ 1.78 (s, 3H), 1.83 (2s, 6H), 2.31-2.46 (m, 1H), 2.55-2.78 (m, 1H), 3.58-3.88 (m, 2H), 4.18-4.29 (m, 1H), 4.41 (br s, 2H), 7.50 (dd, J=8.9, 10.9Hz, 1H), 7.68 (dd, J=2.4, 7.9Hz, 1H), 8.38 (dd, J=3.0, 8.9Hz, 1H), 8.50 (d, J=1.8Hz, 1H), 10.15 (s, 1H). MS(ES+)m/z 533.1[M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺(42AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide (42AA)

步骤1：((4aR，5R，9R)-5-(6-(5-氯-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-89AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(5-chloro-3-methylpicolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-89AA)

将5-氯-3-甲基吡啶甲酸(42.1mg，240μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(42.6mg，29.4μL，336μmol)以及二甲基甲酰胺(0.137M于甲苯中，43.8μL，6μmol)。将混合物在室温搅拌17h。然后，将其在真空中浓缩(40℃，5毫巴)，得到5-氯-3-甲基吡啶甲酰氯，为棕色油状物(48mg，定量的)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AAp，80mg，168μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(41.3ng，55.8μL，320μmol)，接着加入5-氯-3-甲基吡啶甲酰氯(见上，50.6mg，240μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌15min，接着在室温搅拌90min。然后，加入碳酸钠水溶液(10％，15mL)，将混合物在室温搅拌10min。相分离后，将水层用二氯甲烷(2x 10mL)萃取，将合并的有机物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度10∶90至40∶60洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为灰白色固体(61mg，58％)。HPLC(方法LCMS_梯度)t_R＝3.86min。MS(ES+)m/z 629.2[M+H]。5-Chloro-3-methylpicolinic acid (42.1 mg, 240 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (42.6 mg, 29.4 μL, 336 μmol) and dimethylformamide (0.137 M in toluene, 43.8 μL, 6 μmol) were added. The mixture was stirred at room temperature for 17 h. It was then concentrated in vacuo (40° C., 5 mbar) to give 5-chloro-3-methylpicolinic acid chloride as a brown oil (48 mg, quantitative). Subsequently, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AAp, 80 mg, 168 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (41.3 ng, 55.8 μL, 320 μmol) was added, followed by a solution of 5-chloro-3-methylpicolinyl chloride (see above, 50.6 mg, 240 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 15 min and then at room temperature for 90 min. Then, aqueous sodium carbonate (10%, 15 mL) was added and the mixture was stirred at room temperature for 10 min. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 10 mL), and the combined organics were dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 10:90 to 40:60) and dried in vacuo (40° C., 5 mbar) to give the title compound as an off-white solid (61 mg, 58%). HPLC (Method LCMS_Gradient) _tR = 3.86 min. MS (ES+) m/z 629.2 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氯-3-甲基吡啶酰胺(42AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpicolinamide (42AA)

将((4aR，5R，9R)-5-(6-(5-氯-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-89AA，61mg，97μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(192mg，130μL，1.68mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)，并且在搅拌时小心加入氨水(8％m/m，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x 5mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度0∶100至10∶90洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色粉末(49mg，95％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和叔丁基甲醚(3mL)中并浓缩并在真空中在40℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.95min。¹H NMR(CDCl₃，300MHz)：δ1.79(s，3H)，1.84(2s，6H)，2.31-2.46(m，1H)，2.56-2.78(m，1H)，2.81(s，3H)，3.58-3.87(m，2H)，4.18-4.28(m，1H)，4.44(br s，2H)，7.48(dd，J＝8.9，10.9Hz，1H)，7.65-7.70(m，1H)，8.35(dd，J＝3.0，8.9Hz，1H)，8.49(d，J＝1.8Hz，1H)，10.44(s，1H)。MS(ES+)m/z 529.1[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-(5-chloro-3-methylpicolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-89AA, 61 mg, 97 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (192 mg, 130 μL, 1.68 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and aqueous ammonia (8% m/m, 6 mL) was carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 5 mL), the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia/dichloromethane in methanol, gradient 0: 100 to 10: 90) and dried in vacuo (40° C., 5 mbar) to give the title compound as a white powder (49 mg, 95%). For transfer purposes, the material was dissolved in dichloromethane (1 mL) and tert-butyl methyl ether (3 mL) and concentrated and dried in vacuo at 40° C./5 mbar. HPLC (method LCMS_gradient) t _R =1.95 min. ¹ H NMR (CDCl ₃ , 300MHz): δ1.79 (s, 3H), 1.84 (2s, 6H), 2.31-2.46 (m, 1H), 2.56-2.78 (m, 1H), 2.81 (s, 3H), 3.58-3.87 (m, 2H), 4.18-4.28 (m, 1H), 4.44 (br s, 2H), 7.48 (dd, J=8.9, 10.9Hz, 1H), 7.65-7.70 (m, 1H), 8.35 (dd, J=3.0, 8.9Hz, 1H), 8.49 (d, J=1.8Hz, 1H), 10.44 (s, 1H). MS(ES+)m/z 529.1[M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-I1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氯吡啶酰胺(43AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-11,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-chloropicolinamide (43AA)

步骤1：((4aR，5R，9R)-5-(6-(5-氯吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-90AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(5-chloropicolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-90AA)

将5-氯吡啶甲酸(75.6mg，480μmol)悬浮在二氯甲烷(6mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(85.2mg，58.8μL，672μmol)以及二甲基甲酰胺(0.137M于甲苯中，87.6μL，12μmol)。将混合物在室温搅拌17h。然后，将其在真空中浓缩(40℃，5毫巴)，得到5-氯吡啶甲酰氯，为黄色油状物(88.8mg，定量的)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AAp，80mg，168μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(82.6mg，111.6μL，640μmol)，接着加入5-氯吡啶甲酰氯(见上，88.8mg，480μmol)在二氯甲烷(6mL)中的溶液。将反应混合物在0-5℃搅拌15min，接着在室温搅拌3h。然后，加入碳酸钠水溶液(10％，15mL)，将混合物在室温搅拌10min。相分离后，将水层用二氯甲烷(2x 10mL)萃取，将合并的有机物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度10∶90至40∶60洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为灰白色固体(110mg)，其在不进一步纯化的情况下用于下一步骤。HPLC(方法LCMS_梯度)t_R＝3.6min。MS(ES+)m/z 615.1[M+H]。5-Chloropyric acid (75.6 mg, 480 μmol) was suspended in dichloromethane (6 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (85.2 mg, 58.8 μL, 672 μmol) and dimethylformamide (0.137 M in toluene, 87.6 μL, 12 μmol) were added. The mixture was stirred at room temperature for 17 h. It was then concentrated in vacuo (40° C., 5 mbar) to give 5-chloropicolinoyl chloride as a yellow oil (88.8 mg, quantitative). Subsequently, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AAp, 80 mg, 168 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (82.6 mg, 111.6 μL, 640 μmol) was added, followed by a solution of 5-chloropicolinoyl chloride (see above, 88.8 mg, 480 μmol) in dichloromethane (6 mL). The reaction mixture was stirred at 0-5° C. for 15 min and then at room temperature for 3 h. Then, sodium carbonate aqueous solution (10%, 15mL) was added and the mixture was stirred at room temperature for 10min. After phase separation, the aqueous layer was extracted with dichloromethane (2x 10mL), the combined organic matter was dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12g, eluted with ethyl acetate/n-heptane, gradient 10: 90 to 40: 60) and dried in vacuo (40°C, 5 mbar) to obtain the title compound as an off-white solid (110mg), which was used in the next step without further purification. HPLC (method LCMS_gradient) _tR = 3.6min. MS (ES+) m/z 615.1[M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-氯吡啶酰胺(43AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-chloropicolinamide (43AA)

将((4aR，5R，9R)-5-(6-(5-氯吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-90AA，110mg，参见前一步骤，168μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(384mg，259μL，3.36mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)并且在搅拌时小心加入氨水(8％m/m，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x 5mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度0∶100至10∶90洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色粉末(75mg，经2个步骤87％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和叔丁基甲醚(3mL)中并浓缩并在真空中在40℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.8min。¹H NMR(CDCl₃，300MHz)：δ1.79(s，3H)，1.84(2s，6H)，2.30-2.45(m，1H)，2.56-2.79(m，1H)，3.58-3.88(m，2H)，4.17-4.28(m，1H)，4.43(br s，2H)，7.51(dd，J＝8.9，10.9Hz，1H)，7.91(dd，J＝2.3，8.4Hz，1H)，8.27(dd，J＝0.6，8.5Hz，1H)，8.39(dd，J＝3.0，8.9Hz，1H)，8.65(dd，J＝0.6，2.4Hz，1H)，10.26(s，1H)。MS(ES+)m/z 515.1[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-(5-chloropicolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-90AA, 110 mg, see previous step, 168 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (384 mg, 259 μL, 3.36 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and aqueous ammonia (8% m/m, 6 mL) was carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 5 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 0: 100 to 10: 90) and dried in vacuo (40° C., 5 mbar) to give the title compound as a white powder (75 mg, 87% over 2 steps). For transfer purposes, the material was dissolved in dichloromethane (1 mL) and tert-butyl methyl ether (3 mL) and concentrated and dried in vacuo at 40° C./5 mbar. HPLC (method LCMS_gradient) t _R = 1.8 min. ¹ H NMR (CDCl ₃ , 300MHz): δ 1.79 (s, 3H), 1.84 (2s, 6H), 2.30-2.45 (m, 1H), 2.56-2.79 (m, 1H), 3.58-3.88 (m, 2H), 4.17-4.28 (m, 1H), 4.43 (br s, 2H), 7.51 (dd, J=8.9, 10.9Hz, 1H), 7.91 (dd, J=2.3, 8.4Hz, 1H), 8.27 (dd, J=0.6, 8.5Hz, 1H), 8.39 (dd, J=3.0, 8.9Hz, 1H), 8.65 (dd, J=0.6, 2.4Hz, 1H), 10.26 (s, 1H). MS (ES+) m/z 515.1 [M+H].

6-((6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)氨甲酰基)烟酸(44AA)6-((6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)carbamoyl)nicotinic acid (44AA)

步骤1：6-((6-((4aR，5R，9R)-7-((叔丁氧基羰基)氨基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)氨甲酰基)烟酸叔丁酯(Int-91AA)Step 1: tert-Butyl 6-((6-((4aR,5R,9R)-7-((tert-butoxycarbonyl)amino)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)carbamoyl)nicotinate (Int-91AA)

将5-(叔丁氧基羰基)吡啶甲酸(70.4mg，315mol)悬浮在二氯甲烷(5mL)中，将悬浮液冷却至-10-0℃(乙醇/冰浴)并且加入1-氯-N，N，2-三甲基丙烯基胺(98.3mg，736μmol)。在-10-0℃搅拌60min后，在-4℃经5min加入((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AAp，100mg，210tmol)和N，N-二异丙基乙胺(136mg，184μL，1.05mmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌30min并且加温至室温。然后，加入碳酸钠水溶液(10％，15mL)，将混合物在室温搅拌10min。相分离后，将水层用二氯甲烷(2x 20mL)萃取，将合并的有机物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度10∶90至30∶70洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为灰白色固体(140mg，98％)。HPLC(方法LCMS_梯度)t_R＝4.0min。¹HNMR(CDCl₃，300MHz)：δ1.50(s，9H)，1.65(s，9H)，1.83(s，3H)，1.84(s，3H)，1.97(s，3H)，2.53-2.85(m，2H)，3.65-3.92(m，2H)，4.56-4.65(m，1H)，7.64(dd，J＝9.0，10.7Hz，1H)，8.36(d，J＝8.1Hz，1H)，8.45-8.55(m，2H)，9.20-9.24(m，1H)，10.51(s，1H)，11.37(s，1H)。MS(ES+)m/z 681.3[M+H]。5-(tert-Butoxycarbonyl)picolinic acid (70.4 mg, 315 mol) was suspended in dichloromethane (5 mL), the suspension was cooled to -10-0°C (ethanol/ice bath) and 1-chloro-N,N,2-trimethylpropenylamine (98.3 mg, 736 μmol) was added. After stirring at -10-0°C for 60 min, a solution of tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AAp, 100 mg, 210 tmol) and N,N-diisopropylethylamine (136 mg, 184 μL, 1.05 mmol) in dichloromethane (3 mL) was added at -4°C over 5 min. The reaction mixture was stirred at 0-5°C for 30 min and warmed to room temperature. Then, aqueous sodium carbonate solution (10%, 15 mL) was added and the mixture was stirred at room temperature for 10 min. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 20 mL), the combined organics were dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 10: 90 to 30: 70) and dried in vacuo (40° C., 5 mbar) to give the title compound as an off-white solid (140 mg, 98%). HPLC (method LCMS_gradient) t _R = 4.0 min. ^1HNMR (CDCl ₃ , 300MHz): δ1.50 (s, 9H), 1.65 (s, 9H), 1.83 (s, 3H), 1.84 (s, 3H), 1.97 (s, 3H), 2.53-2.85 (m, 2H), 3.65-3.92 (m, 2H), 4.56-4.65 (m, 1H), 7.64 (dd, J=9.0, 10.7Hz, 1H), 8.36 (d, J=8.1Hz, 1H), 8.45-8.55 (m, 2H), 9.20-9.24 (m, 1H), 10.51 (s, 1H), 11.37 (s, 1H). MS(ES+)m/z 681.3[M+H].

步骤2：6-((6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)氨甲酰基)烟酸(44AA)Step 2: 6-((6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)carbamoyl)nicotinic acid (44AA)

将6-((6-((4aR，5R，9R)-7-((叔丁氧基羰基)氨基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)氨甲酰基)烟酸叔丁酯(Int-91AA，140mg，206μmol)溶解在二氯甲烷(7mL)中并且加入三氟乙酸(703mg，475μL，6.17mmol)。将溶液在室温搅拌42h。之后，将混合物在水中浓缩，将泡沫状残留物再溶解在水(5mL)中。加入氢氧化钠水溶液(1M)直到将pH调节至11。然后，加入乙酸直到pH是5。将形成的沉淀过滤出并接着用水，乙腈和二乙醚彻底洗涤，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(86mg，80％)。HPLC(方法LCMS_梯度)t_R＝1.4min。¹HNMR(d6-DMSO，300MHz)：δ1.69(s，3H)，1.71(s，3H)，1.74(s，3H)，2.38-2.42(m，1H)，2.55-2.80(m，1H)，3.42(br s，2H)，3.46-3.70(m，2H)，4.04-4.12(m，1H)，6.83(br s，1H)，7.85(dd，J＝8.9，11.5Hz，1H)，8.20-8.30(m，2H)，8.47(dd，J＝2.0，8.1Hz，1H)，9.14-9.17(m，1H)，10.51(s，1H)。MS(ES+)m/z 525.1[M+H]。Tert-butyl 6-((6-((4aR,5R,9R)-7-((tert-butoxycarbonyl)amino)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)carbamoyl)nicotinate (Int-91AA, 140 mg, 206 μmol) was dissolved in dichloromethane (7 mL) and trifluoroacetic acid (703 mg, 475 μL, 6.17 mmol) was added. The solution was stirred at room temperature for 42 h. Afterwards, the mixture was concentrated in water and the foamy residue was redissolved in water (5 mL). Aqueous sodium hydroxide solution (1 M) was added until the pH was adjusted to 11. Then, acetic acid was added until the pH was 5. The precipitate formed was filtered off and subsequently washed thoroughly with water, acetonitrile and diethyl ether to give, after drying in vacuo (40° C., 5 mbar), the title compound as a white solid (86 mg, 80%). HPLC (method LCMS_gradient) t _R =1.4 min. ¹ HNMR (d6-DMSO, 300MHz): δ1.69 (s, 3H), 1.71 (s, 3H), 1.74 (s, 3H), 2.38-2.42 (m, 1H), 2.55-2.80 (m, 1H), 3.42 (br s, 2H), 3.46-3.70 (m, 2H), 4.04-4.12 (m, 1H), 6.83 (br s, 1H), 7.85 (dd, J=8.9, 11.5Hz, 1H), 8.20-8.30 (m, 2H), 8.47 (dd, J=2.0, 8.1Hz, 1H), 9.14-9.17 (m, 1H), 10.51 (s, 1H). MS (ES+) m/z 525.1 [M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3-氯-5-(二氟甲氧基)吡啶酰胺(45AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-(difluoromethoxy)picolinamide (45AA)

步骤1：((4aR，5R，9R)-5-(6-(3-氯-5-(二氟甲氧基)吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-92AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(3-chloro-5-(difluoromethoxy)picolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-92AA)

将3-氯-5-(二氟甲氧基)吡啶甲酸(56.3mg，252μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(44.8mg，30.9μL，353μmol)以及二甲基甲酰胺(0.242M于甲苯中，26μL，6.3μmol)。将混合物在室温搅拌15h。然后，将其在真空中浓缩(40℃，5毫巴)，得到3-氯-5-(二氟甲氧基)吡啶甲酰氯，为红色油状物(58.4mg，定量的)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AAp，80mg，168μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(41.3mg，55.8μL，320μmol)，接着加入3-氯-5-(二氟甲氧基)吡啶甲酰氯(见上，58.4mg，252μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌45min。然后，加入甲醇(5mL)，将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度10∶90至35∶65洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为灰白色固体(109mg，95％)。HPLC(方法LCMS_fglm)t_R＝1.49min。MS(ES+)m/z 681.3[M+H]。3-Chloro-5-(difluoromethoxy)picolinic acid (56.3 mg, 252 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (44.8 mg, 30.9 μL, 353 μmol) and dimethylformamide (0.242 M in toluene, 26 μL, 6.3 μmol) were added. The mixture was stirred at room temperature for 15 h. It was then concentrated in vacuo (40° C., 5 mbar) to give 3-chloro-5-(difluoromethoxy)picolinic acid chloride as a red oil (58.4 mg, quantitative). Afterwards, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AAp, 80 mg, 168 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (41.3 mg, 55.8 μL, 320 μmol) was added, followed by a solution of 3-chloro-5-(difluoromethoxy)picolinoyl chloride (see above, 58.4 mg, 252 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 45 min. Methanol (5 mL) was then added, and the mixture was stirred at room temperature for 15 min and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 10:90 to 35:65) and dried in vacuo (40° C., 5 mbar) to afford the title compound as an off-white solid (109 mg, 95%). HPLC (Method LCMS_fglm) _tR = 1.49 min. MS (ES+) m/z 681.3 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-3-氯-5-(二氟甲氧基)吡啶酰胺(45AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-(difluoromethoxy)picolinamide (45AA)

将((4aR，5R，9R)-5-(6-(3-氯-5-(二氟甲氧基)吡啶甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-92AA，109mg，160μmol)溶解在二氯甲烷(10mL)中并且加入三氟乙酸(384mg，259μL，3.36mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)，在搅拌时小心加入水(20mL)和氨水(2M，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x 50mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度2∶98至5∶95洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色粉末(91mg，98％)。为了转移的目的，将材料溶解在二氯甲烷(1mL)和叔丁基甲醚(3mL)中并浓缩并在真空中在40℃/5毫巴干燥。HPLC(方法LCMS_梯度)t_R＝1.6min。¹H NMR(CDCl₃，300MHz)：δ1.78(s，3H)，1.82(s，3H)，1.83(s，3H)，2.31-2.46(m，1H)，2.55-2.78(m，1H)，3.58-3.87(m，2H)，4.18-4.29(m，1H)，4.40(br s，2H)，6.68(t，J＝71.2Hz，1H)，7.50(dd，J＝8.9，10.9Hz，1H)，7.71(d，J＝2.2Hz，1H)，8.39(dd，J＝3.0，8.9Hz，1H)，8.49(d，J＝2.4Hz，1H)，10.19(s，1H)。MS(ES+)m/z581.2[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-(3-chloro-5-(difluoromethoxy)picolinamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-92AA, 109 mg, 160 μmol) was dissolved in dichloromethane (10 mL) and trifluoroacetic acid (384 mg, 259 μL, 3.36 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and water (20 mL) and aqueous ammonia (2 M, 6 mL) were carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 50 mL), the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2 M ammonia/dichloromethane in methanol, gradient 2: 98 to 5: 95) and dried in vacuo (40 ° C, 5 mbar) to obtain the title compound as a white powder (91 mg, 98%). For the purpose of transfer, the material was dissolved in dichloromethane (1 mL) and tert-butyl methyl ether (3 mL) and concentrated and dried in vacuo at 40 ° C/5 mbar. HPLC (method LCMS_gradient) t _R = 1.6 min. ¹ H NMR (CDCl ₃ , 300MHz): δ1.78 (s, 3H), 1.82 (s, 3H), 1.83 (s, 3H), 2.31-2.46 (m, 1H), 2.55-2.78 (m, 1H), 3.58-3.87 (m, 2H), 4.18-4.29 (m, 1H), 4.40 (br s, 2H), 6.68 (t, J=71.2Hz, 1H), 7.50 (dd, J=8.9, 10.9Hz, 1H), 7.71 (d, J=2. 2Hz, 1H), 8.39 (dd, J=3.0, 8.9Hz, 1H), 8.49 (d, J=2.4Hz, 1H), 10.19 (s, 1H). MS (ES+) m/z 581.2 [M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-11，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(2，2-二氟乙氧基)吡嗪-2-甲酰胺(46AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-11,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2-difluoroethoxy)pyrazine-2-carboxamide (46AA)

步骤1：((4aR，5R，9R)-5-(6-(5-(2，2-二氟乙氧基)吡嗪-2-甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-93AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(5-(2,2-difluoroethoxy)pyrazine-2-carboxamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-93AA)

5-(2，2-二氟乙氧基)吡嗪-2-甲酸(51.4mg，252μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(44.8mg，30.9μL，353μmol)以及二甲基甲酰胺(0.242M于甲苯中，26μL，6.3μmol)。将混合物在室温搅拌2h。然后，将其在真空中浓缩(40℃，5毫巴)，得到5-(2，2-二氟乙氧基)吡嗪-2-甲酰氯，为黄色油状物(56.1mg，定量的)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AAp，80mg，168μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(41.3mg，55.8μL，320μmol)，接着加入5-(2，2-二氟乙氧基)吡嗪-2-甲酰氯(见上，56.1mg，252μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌75min。然后，加入甲醇(5mL)，将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度10∶90至35∶65洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为灰白色固体(104mg，94％)。HPL℃(方法LCMS_fglm)t_R＝1.49min。MS(ES+)m/z662.3[M+H]。5-(2,2-difluoroethoxy)pyrazine-2-carboxylic acid (51.4 mg, 252 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (44.8 mg, 30.9 μL, 353 μmol) and dimethylformamide (0.242 M in toluene, 26 μL, 6.3 μmol) were added. The mixture was stirred at room temperature for 2 h. It was then concentrated in vacuo (40° C., 5 mbar) to give 5-(2,2-difluoroethoxy)pyrazine-2-carboxylic acid chloride as a yellow oil (56.1 mg, quantitative). Afterwards, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AAp, 80 mg, 168 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (41.3 mg, 55.8 μL, 320 μmol) was added, followed by a solution of 5-(2,2-difluoroethoxy)pyrazine-2-carbonyl chloride (see above, 56.1 mg, 252 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 75 min. Methanol (5 mL) was then added, and the mixture was stirred at room temperature for 15 min and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 10:90 to 35:65) and dried in vacuo (40° C., 5 mbar) to afford the title compound as an off-white solid (104 mg, 94%). HPLC (Method LCMS_fglm) _tR = 1.49 min. MS (ES+) m/z 662.3 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(2，2-二氟乙氧基)吡嗪-2-甲酰胺(46AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2-difluoroethoxy)pyrazine-2-carboxamide (46AA)

将((4aR，5R，9R)-5-(6-(5-(2，2-二氟乙氧基)吡嗪-2-甲酰胺基)-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-93AA，104mg，157μmol)溶解在二氯甲烷(10mL)中并且加入三氟乙酸(384mg，259μL，3.36mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)，在搅拌时小心加入水(20mL)和氨水(2M，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x 50mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度2∶98至5∶95洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色粉末(88mg，99％)。HPLC(方法LCMS_梯度)t_R＝1.7min。¹H NMR(CDCl₃，300MHz)：δ1.79(s，3H)，1.83(s，3H)，1.84(s，3H)，2.29-2.44(m，1H)，2.55-2.79(m，1H)，3.57-3.87(m，2H)，4.16-4.27(m，1H)，4.41(br s，2H)，4.68(dt，J＝4.0，13.3Hz，2H)，6.18(tt，J＝4.0，55.0Hz，1H)，7.51(dd，J＝9.0，10.8Hz，1H)，8.34(d，J＝1.4Hz，1H)，8.38(dd，J＝3.0，8.9Hz，1H)，9.04(d，J＝1.2Hz，1H)，9.94(s，1H)。MS(ES+)m/z562.3[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-(5-(2,2-difluoroethoxy)pyrazine-2-carboxamido)-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-93AA, 104 mg, 157 μmol) was dissolved in dichloromethane (10 mL) and trifluoroacetic acid (384 mg, 259 μL, 3.36 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and water (20 mL) and aqueous ammonia (2 M, 6 mL) were carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 50 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 2:98 to 5:95) to give the title compound as a white powder (88 mg, 99%) after drying in vacuo (40° C., 5 mbar). HPLC (method LCMS_Gradient) t _R =1.7 min. ¹ H NMR (CDCl ₃ , 300MHz): δ1.79 (s, 3H), 1.83 (s, 3H), 1.84 (s, 3H), 2.29-2.44 (m, 1H), 2.55-2.79 (m, 1H), 3.57-3.87 (m, 2H), 4.16-4.27 (m, 1H), 4.41 (br s, 2H), 4.68 (dt, J=4.0, 13.3Hz, 2H), 6.18 (tt, J=4.0, 55.0Hz, 1H), 7.51 (dd, J=9.0, 10.8Hz, 1H), 8.34 (d, J=1.4Hz, 1H), 8.38 (dd, J=3.0, 8.9Hz, 1H), 9.04 (d, J=1.2Hz, 1H), 9.94 (s, 1H). MS(ES+)m/z562.3[M+H].

N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(丙-1-炔-1-基)吡啶酰胺(47AA)N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(prop-1-yn-1-yl)picolinamide (47AA)

步骤1：((4aR，5R，9R)-3，3-二氟-5-(3-氟-6-(5-(丙-1-炔-1-基)吡啶甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-94AA)Step 1: tert-Butyl ((4aR,5R,9R)-3,3-difluoro-5-(3-fluoro-6-(5-(prop-1-yn-1-yl)picolinamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-94AA)

将5-(丙-1-炔-1-基)吡啶甲酸(30.5mg，189μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(33.6mg，23.2μL，265μmol)以及二甲基甲酰胺(0.242M于甲苯中，19.5μL，4.7μmol)。将混合物在室温搅拌3h。然后，将其在真空中浓缩(40℃，5毫巴)，得到5-(丙-1-炔-1-基)吡啶甲酰氯，为黄色油状物(34mg，定量的)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-51AAp，60mg，126μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(31mg，41.9μL，240μmol)，接着加入5-(丙-1-炔-1-基)吡啶甲酰氯(见上，34mg，189μmol)在二氯甲烷(3mL)中溶液的。将反应混合物在0-5℃搅拌75min。然后，加入甲醇(5mL)，将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度10∶90至35∶65洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(85mg)，在不进一步纯化的情况下将其用于下一步骤。HPLC(方法LCMS_fglm)t_R＝1.57min。MS(ES+)m/z 619.3[M+H]。5-(Propan-1-yn-1-yl)picolinic acid (30.5 mg, 189 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (33.6 mg, 23.2 μL, 265 μmol) and dimethylformamide (0.242 M in toluene, 19.5 μL, 4.7 μmol) were added. The mixture was stirred at room temperature for 3 h. It was then concentrated in vacuo (40° C., 5 mbar) to give 5-(prop-1-yn-1-yl)picolinic acid chloride as a yellow oil (34 mg, quantitative). Subsequently, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-51AAp, 60 mg, 126 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (31 mg, 41.9 μL, 240 μmol) was added, followed by a solution of 5-(prop-1-yn-1-yl)picolinoyl chloride (see above, 34 mg, 189 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 75 min. Methanol (5 mL) was then added, and the mixture was stirred at room temperature for 15 min and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 10:90 to 35:65) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a white solid (85 mg), which was used in the next step without further purification. HPLC (Method LCMS_fglm) _tR = 1.57 min. MS (ES+) m/z 619.3 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-3，3-二氟-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-5-基)-5-氟吡啶-2-基)-5-(丙-1-炔-1-基)吡啶酰胺(47AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(prop-1-yn-1-yl)picolinamide (47AA)

将((4aR，5R，9R)-3，3-二氟-5-(3-氟-6-(5-(丙-1-炔-1-基)吡啶甲酰胺基)吡啶-2-基)-5，8，8-三甲基-9-氧代-2，3，4，4a，5，8-六氢-[1，4]噻嗪并[2，1-f][1，2]噻嗪-7-基)氨基甲酸叔丁酯(Int-94AA，85mg，参见前一步骤，126μmol)溶解在二氯甲烷(10mL)中并且加入三氟乙酸(288mg，194μL，2.52mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)，在搅拌时小心加入水(20mL)和氨水(2M，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x50mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度0∶100至7∶93洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(61mg，93％经2个步骤)。HPLC(方法LCMS_梯度)t_R＝1.9min。¹H NMR(CDCl₃，300MHz)：δ1.79(s，3H)，1.84(2s，6H)，2.14(s，3H)，2.29-2.45(m，1H)，2.56-2.79(m，1H)，3.58-3.88(m，2H)，4.16-4.26(m，1H)，4.41(br s，2H)，7.50(dd，J＝8.9，10.9Hz，1H)，7.88(dd，J＝1.9，8.2Hz，1H)，8.22(d，J＝8.1Hz，1H)，8.40(dd，J＝3.0，8.9Hz，1H)，8.66(d，J＝1.4Hz，1H)，10.35(s，1H)。MS(ES+)m/z 519.3[M+H]。Tert-butyl ((4aR,5R,9R)-3,3-difluoro-5-(3-fluoro-6-(5-(prop-1-yn-1-yl)picolinamido)pyridin-2-yl)-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-7-yl)carbamate (Int-94AA, 85 mg, see previous step, 126 μmol) was dissolved in dichloromethane (10 mL) and trifluoroacetic acid (288 mg, 194 μL, 2.52 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and water (20 mL) and aqueous ammonia (2M, 6 mL) were carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 50 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 0: 100 to 7: 93) to give the title compound after drying in vacuo (40° C., 5 mbar) as a white solid (61 mg, 93% over 2 steps). HPLC (Method LCMS_Gradient) t _R = 1.9 min. ¹ H NMR (CDCl ₃ , 300MHz): δ1.79 (s, 3H), 1.84 (2s, 6H), 2.14 (s, 3H), 2.29-2.45 (m, 1H), 2.56-2.79 (m, 1H), 3.58-3.88 (m, 2H), 4.16-4.26 (m, 1H), 4.41 (br s, 2H), 7.50 (dd, J=8.9, 10.9Hz, 1H), 7.88 (dd, J=1.9, 8.2Hz, 1H), 8.22 (d, J= 8.1Hz, 1H), 8.40 (dd, J=3.0, 8.9Hz, 1H), 8.66 (d, J=1.4Hz, 1H), 10.35 (s, 1H). MS (ES+) m/z 519.3 [M+H].

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(48AA)N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (48AA)

步骤1：((4aR，5R，9R)-5-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-95AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-95AA)

将5-氰基-3-甲基吡啶甲酸(52.2mg，322μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(57.2mg，39.5μL，451μmol)以及二甲基甲酰胺(0.242M于甲苯中，33μL，8.0μmol)。将混合物在室温搅拌15h。然后，将其在真空中浓缩(40℃，5毫巴)，得到5-氰基-3-甲基吡啶甲酰氯，为红色油状物(58.2mg，定量的)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-63AA，100mg，215μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(55.5mg，75μL，430μmol)，接着加入5-氰基-3-甲基吡啶甲酰氯(见上，58.2mg，322μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌45min。然后，加入甲醇(5mL)，将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度10∶90至60∶40洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(137mg)，其在不进一步纯化的情况下用于下一步骤。HPLC(方法LCMS_fglm)t_R＝1.45min。MS(ES+)m/z 610.3[M+H]。5-Cyano-3-methylpicolinic acid (52.2 mg, 322 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (57.2 mg, 39.5 μL, 451 μmol) and dimethylformamide (0.242 M in toluene, 33 μL, 8.0 μmol) were added. The mixture was stirred at room temperature for 15 h. It was then concentrated in vacuo (40° C., 5 mbar) to give 5-cyano-3-methylpicolinic acid chloride as a red oil (58.2 mg, quantitative). Subsequently, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-63AA, 100 mg, 215 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (55.5 mg, 75 μL, 430 μmol) was added, followed by a solution of 5-cyano-3-methylpicolinyl chloride (see above, 58.2 mg, 322 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 45 min. Methanol (5 mL) was then added, and the mixture was stirred at room temperature for 15 min and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 10:90 to 60:40) and dried in vacuo (40° C., 5 mbar) to give the title compound as a white solid (137 mg), which was used in the next step without further purification. HPLC (Method LCMS_fglm) _tR = 1.45 min. MS (ES+) m/z 610.3 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(48AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (48AA)

将((4aR，5R，9R)-5-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-95AA，137mg，215μmol，参见前一步骤)溶解在二氯甲烷(10mL)中并且加入三氟乙酸(490mg，331μL，4.3mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)，在搅拌时小心加入水(20mL)和氨水(2M，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x50mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度2∶98至7∶93洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色粉末(100mg)。对映异构纯化通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)60/40，流速1.0mL/min)进行，得到所需主要的，首先洗脱的对映异构体，为白色固体(58mg，52％经2个步骤)，以及相反的，较少的对映异构体，为白色固体(28mg，25％)。HPLC(方法LCMS_梯度)t_R＝1.6min。¹H NMR(CDCl₃，300MHz)：δ0.07-0.17(m，1H)，0.26-0.35(m，1H)，0.39-0.48(m，1H)，0.53-0.62(m，1H)，0.89-0.99(m，1H)，1.83(s，3H)，1.90(s，3H)，1.92(s，3H)，2.46(dd，J＝1.8，13.1Hz，1H)，2.80(dd，J＝13.2，13.2Hz，1H)，2.87(s，3H)，3.96-4.08(m，2H)，5.15(br s，2H)，7.52(dd，J＝8.9，10.9Hz，1H)，7.96-7.99(m，1H)，8.35(dd，J＝3.0，8.9Hz，1H)，8.78-8.81(m，1H)，10.43(s，1H)。MS(ES+)m/z 510.3[M+H]。Tert-butyl ((4aR, 5R, 9R)-5-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a, 5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-95AA, 137 mg, 215 μmol, see previous step) was dissolved in dichloromethane (10 mL) and trifluoroacetic acid (490 mg, 331 μL, 4.3 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and water (20 mL) and aqueous ammonia (2 M, 6 mL) were carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 50 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 2:98 to 7:93) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a white powder (100 mg). Enantiomeric purification was performed by chiral preparative HPLC (Chiralpak AD, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.01% ammonium acetate) 60/40, flow rate 1.0 mL/min) to afford the desired major, first-eluting enantiomer as a white solid (58 mg, 52% over 2 steps) and the opposite, minor enantiomer as a white solid (28 mg, 25%). HPLC (method LCMS_gradient) t _R = 1.6 min. ¹ H NMR (CDCl ₃ , 300MHz): δ0.07-0.17 (m, 1H), 0.26-0.35 (m, 1H), 0.39-0.48 (m, 1H), 0.53-0.62 (m, 1H), 0.89-0.99 (m, 1H), 1.83 (s, 3H), 1.9 0 (s, 3H), 1.92 (s, 3H), 2.46 (dd, J=1.8, 13.1Hz, 1H), 2.80 (dd, J=13.2, 13.2Hz, 1H), 2.87 (s, 3H), 3.96-4.08 (m, 2H), 5.15 (br s, 2H), 7.52 (dd, J=8.9, 10.9Hz, 1H), 7.96-7.99 (m, 1H), 8.35 (dd, J=3.0, 8.9Hz, 1H), 8.78-8.81 (m, 1H), 10.43 (s, 1H). MS(ES+)m/z 510.3[M+H].

N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(48AB)N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (48AB)

步骤1：((4aS，5R，9R)-5-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-95AB)Step 1: tert-Butyl ((4aS,5R,9R)-5-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-95AB)

将5-氰基-3-甲基吡啶甲酸(51.1mg，377μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(67mg，46μL，528μmol)以及二甲基甲酰胺(0.242M于甲苯中，39μL，9.4μmol)。将混合物在室温搅拌15h。然后，将其在真空中浓缩(40℃，5毫巴)，得到5-氰基-3-甲基吡啶甲酰氯，为红色油状物(68.1mg，定量的)。其后，将((4aS，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-63AB，117mg，251μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(65mg，88μL，503μmol)，接着加入5-氰基-3-甲基吡啶甲酰氯(见上，68.1mg，377μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌90min。然后，加入甲醇(5mL)，将混合物在室温搅拌15min。将得到的悬浮液过滤，将沉淀用二氯甲烷(3x5mL)洗涤并干燥，得到第一批标题化合物。将合并的滤液在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度1∶99至3∶97洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到第二批标题化合物。将两批合并，得到标题化合物，为浅棕色固体(141mg，92％)。HPLC(方法LCMS_fglm)t_R＝1.40min。MS(ES+)m/z 610.3[M+H]。5-Cyano-3-methylpicolinic acid (51.1 mg, 377 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (67 mg, 46 μL, 528 μmol) and dimethylformamide (0.242 M in toluene, 39 μL, 9.4 μmol) were added. The mixture was stirred at room temperature for 15 h. It was then concentrated in vacuo (40° C., 5 mbar) to give 5-cyano-3-methylpicolinic acid chloride as a red oil (68.1 mg, quantitative). Subsequently, tert-butyl ((4aS,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-63AB, 117 mg, 251 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (65 mg, 88 μL, 503 μmol) was added, followed by a solution of 5-cyano-3-methylpicolinoyl chloride (see above, 68.1 mg, 377 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 90 min. Then, methanol (5 mL) was added and the mixture was stirred at room temperature for 15 min. The resulting suspension was filtered and the precipitate was washed with dichloromethane (3x5 mL) and dried to give the first batch of the title compound. The combined filtrate was concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in dichloromethane in methanol, gradient 1: 99 to 3: 97) and dried in vacuo (40° C., 5 mbar) to give the second batch of the title compound. The two batches were combined to give the title compound as a light brown solid (141 mg, 92%). HPLC (Method LCMS_fglm) t _R = 1.40 min. MS (ES+) m/z 610.3 [M+H].

步骤2：N-(6-((4aS，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-5-基)-5-氟吡啶-2-基)-5-氰基-3-甲基吡啶酰胺(48AB)Step 2: N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpicolinamide (48AB)

将((4aS，5R，9R)-5-(6-(5-氰基-3-甲基吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-95AB，141mg，231μmol)溶解在二氯甲烷(10mL)中并且加入三氟乙酸(573mg，387μL，5.0mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)，在搅拌时小心加入水(20mL)和氨水(2M，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x50mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度2∶98至7∶93洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(110mg)。对映异构纯化通过手性制备型HPLC(Reprosil Chiral NR，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)60/40，流速1.0mL/min)进行，得到所需(+)-旋光的其次洗脱的对映异构体，为浅棕色固体(68mg，57％)，以及相反(-)-旋光的首先洗脱的对映异构体，为浅棕色固体(22mg，18％)。HPLC(方法LCMS_梯度)t_R＝0.86min。¹H NMR(CDCl₃，300MHz)：δ0.29-0.49(m，3H)，0.69-0.83(m，2H)，1.81(s，3H)，1.93(s，3H)，1.98(s，3H)，2.40(dd，J＝13.9，13.9Hz，1H)，2.79-2.86(m，1H)，2.83(s，3H)，3.83-3.92(m，2H)，6.17(br s，2H)，7.55(dd，J＝9.1，10.3Hz，1H)，7.95-7.98(m，1H)，8.44(dd，J＝3.0，8.9Hz，1H)，8.80(d，J＝1.4Hz，1H)，11.00(s，1H)。MS(ES+)m/z510.2[M+H]。Tert-butyl ((4aS,5R,9R)-5-(6-(5-cyano-3-methylpicolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-95AB, 141 mg, 231 μmol) was dissolved in dichloromethane (10 mL) and trifluoroacetic acid (573 mg, 387 μL, 5.0 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and water (20 mL) and aqueous ammonia (2 M, 6 mL) were carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 50 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 2:98 to 7:93) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a white solid (110 mg). Enantiomeric purification was performed by chiral preparative HPLC (Reprosil Chiral NR, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.01% ammonium acetate) 60/40, flow rate 1.0 mL/min) to afford the desired (+)-optically active second-eluting enantiomer as a light brown solid (68 mg, 57%) and the opposite (-)-optically active first-eluting enantiomer as a light brown solid (22 mg, 18%). HPLC (method LCMS_gradient) t _R =0.86 min. ¹ H NMR (CDCl ₃ , 300 MHz): δ 0.29-0.49 (m, 3H), 0.69-0.83 (m, 2H), 1.81 (s, 3H), 1.93 (s, 3H), 1.98 (s, 3H), 2.40 (dd, J=13.9, 13.9 Hz, 1H), 2.79-2.86 (m, 1H), 2.83 (s, 3H), 3.83-3.92 (m, 2H), 6.17 (br s, 2H), 7.55 (dd, J=9.1, 10.3Hz, 1H), 7.95-7.98 (m, 1H), 8.44 (dd, J=3.0, 8.9Hz, 1H), 8.80 (d, J=1.4Hz, 1H), 11.00 (s, 1H). MS(ES+)m/z510.2[M+H].

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-5-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺(49AA)N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide (49AA)

步骤1：((4aR，5R，9R)-5-(6-(3-氯-5-氰基吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-96AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(3-chloro-5-cyanopicolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-96AA)

将3-氯-5-氰基吡啶甲酸(58.8mg，322μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(57.2mg，39.5μL，451μmol)以及二甲基甲酰胺(0.242M于甲苯中，33μL，8μmol)。将混合物在室温搅拌2h。然后，将其在真空中浓缩(40℃，5毫巴)，得到3-氯-5-氰基吡啶甲酰氯，为棕色油状物(64.8mg，定量的)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-63AA，100mg，215μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(55.5mg，75μL，430μmol)，接着加入3-氯-5-氰基吡啶甲酰氯(见上，64.8mg，322μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌45min。然后，加入甲醇(5mL)，将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度10∶90至70∶30洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为浅黄色固体(137mg，99％)。HPLC(方法LCMS_fglm)t_R＝1.38min。MS(ES+)m/z 630.3[M+H]。3-Chloro-5-cyanopicolinic acid (58.8 mg, 322 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (57.2 mg, 39.5 μL, 451 μmol) and dimethylformamide (0.242 M in toluene, 33 μL, 8 μmol) were added. The mixture was stirred at room temperature for 2 h. It was then concentrated in vacuo (40° C., 5 mbar) to give 3-chloro-5-cyanopicolinic acid chloride as a brown oil (64.8 mg, quantitative). Subsequently, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-63AA, 100 mg, 215 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (55.5 mg, 75 μL, 430 μmol) was added, followed by a solution of 3-chloro-5-cyanopicolinyl chloride (see above, 64.8 mg, 322 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 45 min. Methanol (5 mL) was then added, and the mixture was stirred at room temperature for 15 min and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 10:90 to 70:30) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a light yellow solid (137 mg, 99%). HPLC (Method LCMS_fglm) _tR = 1.38 min. MS (ES+) m/z 630.3 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-5-基)-5-氟吡啶-2-基)-3-氯-5-氰基吡啶酰胺(49AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopicinamide (49AA)

将((4aR，5R，9R)-5-(6-(3-氯-5-氰基吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-96AA，137mg，214μmol)溶解在二氯甲烷(10mL)中并且加入三氟乙酸(490mg，331μL，4.3mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)，在搅拌时小心加入水(20mL)和氨水(2M，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x50mL)萃取，将合并的萃取物干燥(硫酸钠)并在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度0∶100至8∶92洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(97mg)。对映异构纯化通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)60/40，流速1.0mL/min)进行，得到所需首先洗脱的对映异构体，为灰白色固体(67mg，58％)，以及相反其次洗脱的对映异构体，为浅棕色固体(26mg，22％)。HPLC(方法LCMS_梯度)t_R＝1.5min。¹H NMR(CDCl₃，300MHz)：δ0.02-0.10(m，1H)，0.27-0.36(m，1H)，0.40-0.49(m，1H)，0.51-0.60(m，1H)，0.84-0.96(m，1H)，1.87(s，3H)，1.91(s，3H)，1.95(s，3H)，2.42-2.51(m，1H)，2.80(dd，J＝13.2，13.2Hz，1H)，3.92-4.02(m，2H)，4.62(br s，2H)，7.57(dd，J＝9.0，11.0Hz，1H)，8.21(d，J＝1.8Hz，1H)，8.41(dd，J＝3.0，8.9Hz，1H)，8.85(d，J＝1.8Hz，1H)，10.16(s，1H)。MS(ES+)m/z 530.3[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-(3-chloro-5-cyanopicolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-96AA, 137 mg, 214 μmol) was dissolved in dichloromethane (10 mL) and trifluoroacetic acid (490 mg, 331 μL, 4.3 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and water (20 mL) and aqueous ammonia (2 M, 6 mL) were carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2 x 50 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 0:100 to 8:92) and dried in vacuo (40° C., 5 mbar) to afford the title compound as a white solid (97 mg). Enantiomeric purification was performed by chiral preparative HPLC (Chiralpak AD, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.01% ammonium acetate) 60/40, flow rate 1.0 mL/min) to afford the desired first-eluting enantiomer as an off-white solid (67 mg, 58%) and the opposite second-eluting enantiomer as a light brown solid (26 mg, 22%). HPLC (Method LCMS_Gradient) _tR = 1.5 min. ¹ H NMR (CDCl ₃ , 300MHz): δ0.02-0.10 (m, 1H), 0.27-0.36 (m, 1H), 0.40-0.49 (m, 1H), 0.51-0.60 (m, 1H), 0.84-0.96 (m, 1H), 1.87 (s, 3H), 1.91 (s, 3H), 1.95 (s, 3H), 2.42-2.51 (m, 1H), 2.80 (dd, J=13.2, 13.2Hz, 1H), 3.92-4.02 (m, 2H), 4.62 (br s, 2H), 7.57 (dd, J=9.0, 11.0Hz, 1H), 8.21 (d, J=1.8Hz, 1H), 8.41 (dd, J=3.0, 8.9Hz, 1H), 8.85 (d, J=1.8Hz, 1H), 10.16 (s, 1H). MS(ES+)m/z 530.3[M+H].

N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-5-基)-5-氟吡啶-2-基)-3-氯-5-(二氟-甲氧基)吡啶酰胺(50AA)N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-(difluoro-methoxy)picolinamide (50AA)

步骤1：((4aR，5R，9R)-5-(6-(3-氯-5-(二氟甲氧基)吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-97AA)Step 1: tert-Butyl ((4aR,5R,9R)-5-(6-(3-chloro-5-(difluoromethoxy)picolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-97AA)

将3-氯-5-(二氟甲氧基)吡啶甲酸(72mg，322μmol)悬浮在二氯甲烷(3mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(57.2mg，39.5μL，451μmol)以及二甲基甲酰胺(0.242M于甲苯中，33μL，8μmol)。将混合物在室温搅拌2h。然后，将其在真空中浓缩(40℃，5毫巴)，得到3-氯-5-(二氟甲氧基)吡啶甲酰氯，为黄色油状物(78mg，定量的)。其后，将((4aR，5R，9R)-5-(6-氨基-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-63AA，100mg，215μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(55.5mg，75μL，430μmol)，接着将3-氯-5-(二氟甲氧基)吡啶甲酰氯(见上，78mg，322μmol)在二氯甲烷(3mL)中的溶液。将反应混合物在0-5℃搅拌45min。然后，加入甲醇(5mL)，将混合物在室温搅拌15min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用乙酸乙酯/正庚烷，梯度10∶90至60∶40洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为灰白色固体(137mg，95％)。HPLC(方法LCMS_fglm)t_R＝1.48min。MS(ES+)m/z 671.3[M+H]。3-Chloro-5-(difluoromethoxy)picolinic acid (72 mg, 322 μmol) was suspended in dichloromethane (3 mL), the suspension was cooled to 0-5° C. (ice bath) and oxalyl chloride (57.2 mg, 39.5 μL, 451 μmol) and dimethylformamide (0.242 M in toluene, 33 μL, 8 μmol) were added. The mixture was stirred at room temperature for 2 h. It was then concentrated in vacuo (40° C., 5 mbar) to give 3-chloro-5-(difluoromethoxy)picolinic acid chloride as a yellow oil (78 mg, quantitative). Afterwards, tert-butyl ((4aR,5R,9R)-5-(6-amino-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-63AA, 100 mg, 215 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (55.5 mg, 75 μL, 430 μmol) was added, followed by a solution of 3-chloro-5-(difluoromethoxy)picolinoyl chloride (see above, 78 mg, 322 μmol) in dichloromethane (3 mL). The reaction mixture was stirred at 0-5° C. for 45 min. Methanol (5 mL) was then added, and the mixture was stirred at room temperature for 15 min and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with ethyl acetate/n-heptane, gradient 10:90 to 60:40) and dried in vacuo (40° C., 5 mbar) to afford the title compound as an off-white solid (137 mg, 95%). HPLC (Method LCMS_fglm) _tR = 1.48 min. MS (ES+) m/z 671.3 [M+H].

步骤2：N-(6-((4aR，5R，9R)-7-氨基-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-5-基)-5-氟吡啶-2-基)-3-氯-5-(二氟甲氧基)吡啶酰胺(50AA)Step 2: N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-(difluoromethoxy)picolinamide (50AA)

将((4aR，5R，9R)-5-(6-(3-氯-5-(二氟甲氧基)吡啶甲酰胺基)-3-氟吡啶-2-基)-5，8，8-三甲基-9-氧代-4，4a，5，8-四氢-2H-螺[[1，4]噻嗪并[2，1-f][1，2]噻嗪-3，1′-环丙]-7-基)氨基甲酸叔丁酯(Int-97AA，137mg，204μmol)溶解在二氯甲烷(10mL)中并且加入三氟乙酸(490mg，331μL，4.3mmol)。将溶液在室温搅拌17h。之后，将混合物冷却至0-5℃(冰浴)，在搅拌时小心加入水(20mL)和氨水(2M，6mL)，直到水层的pH是11-12。相分离后，将水层用二氯甲烷(2x50mL)萃取，将合并的萃取物干燥(硫酸钠)并且在真空中浓缩。将残留物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度0∶100至8∶92洗脱)纯化，在真空中干燥(40℃，5毫巴)后，得到标题化合物，为白色固体(108mg)。对映异构纯化通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)60/40，流速1.0mL/min)进行，得到所需首先洗脱的对映异构体，为灰白色固体(80mg，69％)，以及相反其次洗脱的对映异构体，为浅棕色固体(25mg，21％)。HPLC(方法LCMS_梯度)t_R＝1.8min。¹HNMR(CDCl₃，300MHz)：δ0.06-0.16(m，1H)，0.26-0.36(m，1H)，0.39-0.47(m，1H)，0.52-0.61(m，1H)，0.89-0.98(m，1H)，1.83(s，3H)，1.90(s，3H)，1.92(s，3H)，2.42-2.49(m，1H)，2.79(dd，J＝13.3，13.3Hz，1H)，3.96-4.06(m，2H)，5.67(br s，2H)，6.68(t，J＝71.1Hz，1H)，7.52(dd，J＝8.9，10.9Hz，1H)，7.72(d，J＝2.4Hz，1H)，8.39(dd，J＝3.0，8.9Hz，1H)，8.48(d，J＝2.2Hz，1H)，10.19(s，1H)。MS(ES+)m/z 571.2[M+H]。Tert-butyl ((4aR,5R,9R)-5-(6-(3-chloro-5-(difluoromethoxy)picolinamido)-3-fluoropyridin-2-yl)-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-7-yl)carbamate (Int-97AA, 137 mg, 204 μmol) was dissolved in dichloromethane (10 mL) and trifluoroacetic acid (490 mg, 331 μL, 4.3 mmol) was added. The solution was stirred at room temperature for 17 h. Afterwards, the mixture was cooled to 0-5° C. (ice bath) and water (20 mL) and aqueous ammonia (2 M, 6 mL) were carefully added while stirring until the pH of the aqueous layer was 11-12. After phase separation, the aqueous layer was extracted with dichloromethane (2x50 mL), and the combined extracts were dried (sodium sulfate) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 0:100 to 8:92) and dried in vacuo (40°C, 5 mbar) to give the title compound as a white solid (108 mg). Enantiomeric purification was performed by chiral preparative HPLC (Chiralpak AD, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.01% ammonium acetate) 60/40, flow rate 1.0 mL/min) to give the desired first-eluting enantiomer as an off-white solid (80 mg, 69%) and the opposite second-eluting enantiomer as a light brown solid (25 mg, 21%). HPLC (Method LCMS_Gradient) _tR = 1.8 min. ^1HNMR (CDCl ₃ , 300MHz): δ0.06-0.16 (m, 1H), 0.26-0.36 (m, 1H), 0.39-0.47 (m, 1H), 0.52-0.61 (m, 1H), 0.89-0.98 (m, 1H), 1.83 (s, 3H), 1.90 (s, 3H), 1.92 (s, 3H), 2.42-2.49 (m, 1H), 2.79 (dd, J=13.3, 13.3Hz, 1H), 3.96-4.06 (m, 2H), 5.67 (br s, 2H), 6.68 (t, J=71.1Hz, 1H), 7.52 (dd, J=8.9, 10.9Hz, 1H), 7.72 (d, J=2. 4Hz, 1H), 8.39 (dd, J=3.0, 8.9Hz, 1H), 8.48 (d, J=2.2Hz, 1H), 10.19 (s, 1H). MS(ES+)m/z 571.2[M+H].

N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-4-氯-1-(二氟甲基)-1H-吡唑-3-甲酰胺(51AB)N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-4-chloro-1-(difluoromethyl)-1H-pyrazole-3-carboxamide (51AB)

步骤1：((3aS，4R，8R)-4-(6-(4-氯-1-(二氟甲基)-1H-吡唑-3-甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-98AB)Step 1: tert-Butyl ((3aS,4R,8R)-4-(6-(4-chloro-1-(difluoromethyl)-1H-pyrazole-3-carboxamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-98AB)

将4-氯-1-(二氟甲基)-1H-吡唑-3-甲酸(111mg，565μmol)悬浮在二氯甲烷(5mL)中，将悬浮液冷却至0-5℃(冰浴)并且加入草酰氯(100mg，791μmol)以及二甲基甲酰胺(0.308M于甲苯中，76μL，23μmol)。将混合物在室温搅拌3.5h。然后，将其在真空中浓缩(40℃，5毫巴)并且通过加入甲苯(5mL)共沸干燥，接着在真空中浓缩，得到4-氯-1-(二氟甲基)-1H-吡唑-3-碳酰氯，为浅黄色油状物(121mg，定量的)。其后，将((3aS，4R，8R)-4-(6-氨基-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-39AB，100mg，235μmol)溶解在二氯甲烷(5mL)中，将溶液冷却至0-5℃(冰浴)并且加入N，N-二异丙基乙胺(46mg，62μL，353μmol)和4-(二甲基氨基)吡啶(2.9mg，23.5μmol)，接着加入4-氯-1-(二氟甲基)-1H-吡唑-3-碳酰氯(见上，61mg，282μmol)在二氯甲烷(5mL)中的溶液。将反应混合物在室温搅拌75min。然后，加入另外部分的N，N-二异丙基乙胺(46mg，62μL，353μmol)和4-氯-1-(二氟甲基)-1H-吡唑-3-碳酰氯(见上，60mg，280μmol)在二氯甲烷(5mL)中的溶液，将混合物在室温搅拌16h。之后，加入第三部分的N，N-二异丙基乙胺(46mg，62μL，353μmol)和4-氯-1-(二氟甲基)-1H-吡唑-3-碳酰氯(见上，61mg，282μmol)在二氯甲烷(5mL)中的溶液，将混合物在室温搅拌另外1h。加入碳酸钠水溶液(10％m/m，25mL)，将水相用二氯甲烷(2x25mL)萃取，将合并的有机萃取物干燥(硫酸钠)并在真空中浓缩。将粗产物通过柱色谱(硅胶，24g，用乙酸乙酯/二氯甲烷，梯度35∶65至100∶0洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为灰白色固体(71mg，50％产率)。HPLC(方法LCMS_fglm)t_R＝1.31min。MS(ES+)m/z 604.4[M+H]。4-Chloro-1-(difluoromethyl)-1H-pyrazole-3-carboxylic acid (111 mg, 565 μmol) was suspended in dichloromethane (5 mL), the suspension was cooled to 0-5 ° C (ice bath) and oxalyl chloride (100 mg, 791 μmol) and dimethylformamide (0.308 M in toluene, 76 μL, 23 μmol) were added. The mixture was stirred at room temperature for 3.5 h. It was then concentrated in vacuo (40 ° C, 5 mbar) and azeotropically dried by adding toluene (5 mL), followed by concentration in vacuo to give 4-chloro-1-(difluoromethyl)-1H-pyrazole-3-carbonyl chloride as a light yellow oil (121 mg, quantitative). Afterwards, tert-butyl ((3aS,4R,8R)-4-(6-amino-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-39AB, 100 mg, 235 μmol) was dissolved in dichloromethane (5 mL), the solution was cooled to 0-5° C. (ice bath) and N,N-diisopropylethylamine (46 mg, 62 μL, 353 μmol) and 4-(dimethylamino)pyridine (2.9 mg, 23.5 μmol) were added, followed by a solution of 4-chloro-1-(difluoromethyl)-1H-pyrazole-3-carbonyl chloride (see above, 61 mg, 282 μmol) in dichloromethane (5 mL). The reaction mixture was stirred at room temperature for 75 min. Then, additional portions of N,N-diisopropylethylamine (46 mg, 62 μL, 353 μmol) and a solution of 4-chloro-1-(difluoromethyl)-1H-pyrazole-3-carbonyl chloride (see above, 60 mg, 280 μmol) in dichloromethane (5 mL) were added, and the mixture was stirred at room temperature for 16 h. Afterwards, a third portion of N,N-diisopropylethylamine (46 mg, 62 μL, 353 μmol) and a solution of 4-chloro-1-(difluoromethyl)-1H-pyrazole-3-carbonyl chloride (see above, 61 mg, 282 μmol) in dichloromethane (5 mL) was added, and the mixture was stirred at room temperature for another 1 h. Aqueous sodium carbonate solution (10% m/m, 25 mL) was added, the aqueous phase was extracted with dichloromethane (2 x 25 mL), and the combined organic extracts were dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 24 g, eluted with ethyl acetate/dichloromethane, gradient 35:65 to 100:0) to afford the title compound as an off-white solid (71 mg, 50% yield) after drying in vacuo (40° C., 5 mbar). HPLC (Method LCMS_fglm) _tR = 1.31 min. MS (ES+) m/z 604.4 [M+H].

步骤2：N-(6-((3aS，4R，8R)-6-氨基-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-4-基)-5-氟吡啶-2-基)-4-氯-1-(二氟甲基)-1H-吡唑-3-甲酰胺(51AB)Step 2: N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-4-chloro-1-(difluoromethyl)-1H-pyrazole-3-carboxamide (51AB)

将((3aS，4R，8R)-4-(6-(4-氯-1-(二氟甲基)-1H-吡唑-3-甲酰胺基)-3-氟吡啶-2-基)-4，7，7-三甲基-8-氧代-3，3a，4，7-四氢-2H-异噻唑并[1，5-a][1，4]噻嗪-6-基)氨基甲酸叔丁酯(Int-98AB，71mg，118μmol)溶解在二氯甲烷(5mL)中并且加入三氟乙酸(268mg，181μL，2.35mmol)。将溶液在室温搅拌18h。之后，将混合物在真空中浓缩，将残留物再溶解在甲醇(5mL)中，加入氨水(25％m/m，200μL)，在室温搅拌5min，并在真空中浓缩。将粗产物通过柱色谱(硅胶，12g，用甲醇中2M氨/二氯甲烷，梯度1∶99至6∶94洗脱)纯化，在真空中干燥(40℃，5毫巴)后得到标题化合物，为白色固体(60mg)。对映异构纯化通过手性制备型HPLC(Chiralpak AD，250*4.6mm*5μm，等度，正庚烷/(乙醇+0.01％乙酸铵)80/20，流速1.0mL/min)进行，得到所需其次洗脱的对映异构体，为灰白色粉末(40mg，67％)，以及相反首先洗脱的对映异构体，为灰白色粉末(10mg，17％)。HPLC(方法LCMS_梯度)t_R＝1.4min。¹HNMR(CDCl₃，300MHz)：δ1.62-1.78(m，1H)，1.80(s，3H)，1.93(s，3H)，2.03-2.14(m，1H)，2.06(s，3H)，3.52(dd，J＝7.5，10.5Hz，1H)，3.71(ddd，J＝5.0，10.6，10.6Hz，1H)，4.23(ddd，J＝2.1，7.1，12.4Hz，1H)，7.24(t，J＝59.8Hz，1H)，7.58(dd，J＝8.9，10.3Hz，1H)，7.97(s，1H)，8.43(dd，J＝3.0，8.9Hz，1H)，9.35(s，1H)。MS(ES+)m/z 504.3[M+H]。Tert-butyl ((3aS,4R,8R)-4-(6-(4-chloro-1-(difluoromethyl)-1H-pyrazole-3-carboxamido)-3-fluoropyridin-2-yl)-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazolo[1,5-a][1,4]thiazin-6-yl)carbamate (Int-98AB, 71 mg, 118 μmol) was dissolved in dichloromethane (5 mL) and trifluoroacetic acid (268 mg, 181 μL, 2.35 mmol) was added. The solution was stirred at room temperature for 18 h. Afterwards, the mixture was concentrated in vacuo and the residue was redissolved in methanol (5 mL), ammonia (25% m/m, 200 μL) was added, stirred at room temperature for 5 min, and concentrated in vacuo. The crude product was purified by column chromatography (silica gel, 12 g, eluted with 2M ammonia in methanol/dichloromethane, gradient 1:99 to 6:94) to afford the title compound after drying in vacuo (40° C., 5 mbar) as a white solid (60 mg). Enantiomeric purification was performed by chiral preparative HPLC (Chiralpak AD, 250*4.6 mm*5 μm, isocratic, n-heptane/(ethanol+0.01% ammonium acetate) 80/20, flow rate 1.0 mL/min) to afford the desired second-eluting enantiomer as an off-white powder (40 mg, 67%) and the opposite first-eluting enantiomer as an off-white powder (10 mg, 17%). HPLC (method LCMS_gradient) t _R = 1.4 min. ¹ H NMR (CDCl ₃ , 300MHz): δ1.62-1.78 (m, 1H), 1.80 (s, 3H), 1.93 (s, 3H), 2.03-2.14 (m, 1H) , 2.06 (s, 3H), 3.52 (dd, J=7.5, 10.5Hz, 1H), 3.71 (ddd, J=5.0, 10.6, 10.6Hz , 1H), 4.23 (ddd, J=2.1, 7.1, 12.4Hz, 1H), 7.24 (t, J=59.8Hz, 1H), 7.58 (dd, J=8.9, 10.3Hz, 1H), 7.97 (s, 1H), 8.43 (dd, J=3.0, 8.9Hz, 1H), 9.35 (s, 1H). MS (ES+) m/z 504.3 [M+H].

可以类似地合成实施例2BB，3AB，3BA，3BB，4AB，4BA，4BB，5AA，5AB，5BA，5BB，6AB，6BA，6BB，7AB，7BA，7BB，8AB，8BA，8BB，17AB，17BA，17BB，18AA，18AB，18BA，18BB，19AA，19AB，19BA，20AA，20AB，20BA，20BB，21AA，21AB，21BA，21BB，22AA，22AB，22BA，22BB，23AA，23AB，23BA，23BB，24AA，24AB，24BA和24BB。Examples 2BB, 3AB, 3BA, 3BB, 4AB, 4BA, 4BB, 5AA, 5AB, 5BA, 5BB, 6AB, 6BA, 6BB, 7AB, 7BA, 7BB, 8AB, 8BA, 8BB, 17AB, 17BA, 17BB, 18AA, 18AB, 18BA, 18BB, 19AA, 19AB, 19BA, 20AA, 20AB, 20BA, 20BB, 21AA, 21AB, 21BA, 21BB, 22AA, 22AB, 22BA, 22BB, 23AA, 23AB, 23BA, 23BB, 24AA, 24AB, 24BA and 24BB can be synthesized similarly.

Claims

1. A compound of formula I,

in

n is 1, 2, or 3;

^R1 Choose from the following groups.

i) _C1-6 -alkyl, and

ii) Halogen- _C1-6 -alkyl;

^R2 Choose from the following groups

i) _C1-6 -alkyl, and

ii) Halogen- _C1-6 -alkyl;

Or ^R1 and ^R2 together with the C- atoms to which they are attached form a _C3-6 -cycloalkyl-, wherein the _C3-6 -cycloalkyl- is optionally substituted by one or more substituents selected from the group consisting of halogens and hydroxyl groups;

^R3 Each group is independently selected from the following items.

i) Hydrogen,

ii) _C1-6 -alkyl, and

iii) Halogens;

^R4 Each of the following groups can be chosen independently.

i) Hydrogen,

ii) _C1-6 -alkyl, and

iii) Halogens;

Or, ^R3 and ^R4 together are -( _CH2 ) _m- , where m is 2, 3, 4, or 5.

^R5 is hydrogen.

^R6 Choose from the following groups.

i) _C1-6 -alkyl, and

ii _{) Halogen-C1-6} _- alkyl;

^R7 Choose from the following groups.

i) Hydrogen, and

ii) Halogens;

^R8 selects a group consisting of the following items.

i) Aryl

ii) An aryl group substituted with 1 to 4 substituents individually selected from the following: amino, cyano, halogen, halogen- _C1-6 -alkyl, halogen- _C1-6 -alkoxy, _C1-6 -alkoxy, _C1-6 -alkoxy- _{C1-6-alkyl, C2-6} _- alkynyl- _C1-6 -alkoxy, _C2-6 -alkynyl, _C1-6 -alkyl, wherein ^R9 is H or _C1-6 -alkyl (COOR ⁹⁾ , wherein ^R10 is H or _C1-6 -alkyl ( _C3-6 -cycloalkyl) and ^R11 is H or _C1-6 -alkyl (CONR ¹⁰ R11 ⁾ , optionally substituted with 1 _to 4 substituents individually selected from the group consisting of halogen, cyano, _C1-6 -alkyl and _C1-6 -alkoxy, and _C3-6 -cycloalkyl- _C1-6 -alkoxy, wherein the cycloalkyl unit is substituted by one to four substituents individually selected from the group consisting of halogen, cyano, _C1-6 -alkyl and _C1-6 -alkoxy;

iii) heteroaryl, and

iv) Heteroaryl groups substituted with 1 to 4 substituents individually selected from the following: amino, cyano, halogen, halogen- _C1-6 -alkyl, halogen- _C1-6 -alkoxy, _C1-6 -alkoxy, _{C1-6-alkoxy-C1-6} _{-alkyl, C2-6} _- alkynyl- _C1-6 -alkoxy, _C2-6 -alkynyl, _C1-6 -alkyl, wherein ^R9 is H or _C1-6 -alkyl (COOR ⁹⁾ , wherein ^R10 is H or _C1-6 -alkyl ( _C3-6 -cycloalkyl) and ^R11 is H or _C1-6 -alkyl (CONR ¹⁰ R11 ⁾ , optionally substituted with 1 to 4 substituents individually selected from the group consisting of halogen, cyano, _C1-6 - _alkyl and _C1-6 -alkoxy, and _C3-6 -cycloalkyl- _C1-6 -alkoxy, wherein the cycloalkyl unit is substituted by one to four substituents individually selected from the group consisting of halogen, cyano, _C1-6 -alkyl and _C1-6 -alkoxy;

"Aryl" refers to a ring system consisting of a monovalent aromatic carbon ring, either monocyclic or bicyclic, containing 6 to 10 carbon ring atoms.

"Heteroaryl" refers to an aromatic carbocyclic group having a single 5- to 8-membered ring, or multiple fused rings containing 6 to 10 ring atoms, and containing 1, 2, or 3 heteroatoms independently selected from N, O, and S, wherein at least one heterocycle in the group is aromatic, or a medicinal salt thereof.

2. The compound of formula I according to claim 1, wherein...

n is 1, 2, or 3;

^R1 Choose from the following groups.

i) _C1-6 -alkyl, and

ii) Halogen- _C1-6 -alkyl;

^R2 Choose from the following groups

i) _C1-6 -alkyl, and

ii) Halogen- _C1-6 -alkyl;

Or ^R1 and ^R2 together with the C-atoms they are attached to form a _C3-6 -cycloalkyl-, wherein the _C3-6 -cycloalkyl- is optionally substituted by one or more substituents selected from the group consisting of halogens and hydroxyl groups;

^R3 Each group is independently selected from the following items.

i) Hydrogen,

ii) _C1-6 -alkyl, and

iii) Halogens;

^R4 Each of the following groups can be chosen independently.

i) Hydrogen,

ii) _C1-6 -alkyl, and

iii) Halogens;

^R5 is hydrogen.

^R6 Choose from the following groups.

i) _C1-6 -alkyl, and

ii) Halogen- _C1-6 -alkyl;

^R7 Choose from the following groups.

i) Hydrogen, and

ii) Halogens;

^R8 selects a group consisting of the following items.

i) Aryl

ii) an aryl group substituted with 1 to 4 substituents individually selected from the group consisting of: amino, cyano, halogen, halogen- _C1-6 -alkyl, halogen- _C1-6 -alkoxy, _C1-6 -alkoxy, _C1-6 -alkoxy- _C1-6 -alkyl, _C2-6 -alkynyl- _C1-6 -alkoxy, _C2-6 -alkynyl, _C1-6 -alkyl, _C3-6 -cycloalkyl, optionally substituted with 1 to 4 substituents individually selected from the group consisting of halogen, cyano, _C1-6 -alkyl and _C1-6 -alkoxy, and _C3-6 -cycloalkyl _-C1-6 _- alkoxy, wherein the cycloalkyl unit is substituted with 1 to 4 substituents individually selected from the group consisting of halogen, cyano, _C1-6 -alkyl and _C1-6 -alkoxy;

iii) heteroaryl, and

iv) A heteroaryl group substituted with 1 to 4 substituents individually selected from the group consisting of: amino, cyano, halogen, halogen- _C1-6 -alkyl, halogen- _C1-6 -alkoxy, _C1-6 -alkoxy, _C1-6 -alkoxy- _C1-6 -alkyl, _C2-6 -alkynyl- _C1-6 -alkoxy, _C2-6 -alkynyl, _{C1-6-alkyl, C3-6} _- cycloalkyl, optionally substituted with 1 to 4 substituents individually selected from the group consisting of halogen, cyano, _C1-6 -alkyl and _C1-6 -alkoxy, and _C3-6 -cycloalkyl _-C1-6 _- alkoxy, wherein the cycloalkyl unit is substituted with 1 to 4 substituents individually selected from the group consisting of halogen, cyano, _C1-6 -alkyl and _C1-6 -alkoxy;

Wherein “aryl” and “heteroaryl” are as defined in claim 1,

Or its medicinal salt.

3. The compound of formula I according to any one of claims 1-2, wherein it is a compound of formula Ia, wherein n, ^R1 , ^R2 , ^R3 , ^R4 , ^R5 , ^R6 , ^R7 and ^R8 are as described in any one of claims 1-2.

4. The compound of formula I according to any one of claims 1-2, wherein ^R1 is methyl.

5. The compound of formula I according to any one of claims 1-2, wherein ^R2 is methyl.

6. The compound of formula I according to any one of claims 1-2, wherein ^R3 is hydrogen.

7. The compound of formula I according to any one of claims 1-2, wherein ^R4 is hydrogen.

8. The compound of formula I according to any one of claims 1-2, wherein ^R3 and ^R4 together are -( _CH2 ) _2- .

9. The compound of formula I according to any one of claims 1-2, wherein ^R5 is hydrogen.

10. The compound of formula I according to any one of claims 1-2, wherein ^R6 is methyl.

11. The compound of formula I according to any one of claims 1-2, wherein ^R7 is F.

12. The compound of formula I according to any one of claims 1-2, wherein ^R8 is an aryl group substituted with 1-2 substituents individually selected from cyano and halogen.

13. The compound of formula I according to any one of claims 1-2, wherein ^R8 is a phenyl group substituted with 1-2 substituents individually selected from cyano and Cl.

14. The compound of formula I according to any one of claims 1-2, wherein ^R8 is a heteroaryl group substituted with 1-2 substituents individually selected from cyano, halogen, halogen- _C1-6 -alkoxy, halogen- _C1-6 -alkyl, _C1-6 -alkoxy, _C2-6 -alkynyl and _C1-6 -alkyl.

15. The compound of formula I according to any one of claims 1-2, wherein ^R8 is a heteroaryl group substituted with 1-2 substituents individually selected from cyano, halogen, halogen- _C1-6 -alkoxy, _C1-6 -alkoxy and _C1-6 -alkyl.

16. The compound of formula I according to any one of claims 1-2, wherein ^R8 is 1H-pyrazolyl, pyridyl, pyrazinyl or imidazo[1,2-a]pyridyl, which is substituted by 1-2 substituents individually selected from cyano, halogen, halogen- _C1-6 -alkoxy, halogen- _C1-6 -alkyl, _C1-6 -alkoxy, _C2-6 -alkynyl and _C1-6 -alkyl.

17. The compound of formula I according to any one of claims 1-2, wherein ^R8 is pyridyl, pyrazinyl or imidazo[1,2-a]pyridyl, each of which is substituted by 1-2 substituents individually selected from cyano, halogen, halogen- _C1-6 -alkoxy, _C1-6 -alkoxy and _C1-6 -alkyl.

18. The compound of formula I according to any one of claims 1-2, wherein n is 1 or 2.

19. The compound of formula I according to any one of claims 1-2, wherein the compound is selected from the group consisting of the following:

N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpyridineamide,

N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinamide,

N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpyridineamide,

N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)pyridineamide,

N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyridineamide,

N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)pyridineamide,

N-(6-((3aR,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazin-2-carboxamide,

N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide,

N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpyridineamide,

N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinamide,

N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpyridineamide,

N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)pyridineamide,

N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyridineamide,

N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)pyridineamide,

N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazin-2-carboxamide,

N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyrazin-2-carboxamide,

N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoropyridinamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpyridineamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpyridineamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)pyridineamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyridineamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)pyridineamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazin-2-carboxamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyrazin-2-carboxamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoropyridinamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-(difluoromethoxy)pyridineamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridineamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-4-cyanobenzamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-2-chloro-4-cyanobenzamide,

N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpyridineamide,

N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinamide,

N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpyridineamide,

N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)pyridineamide,

N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyridineamide,

N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)pyridineamide,

N-(6-((3aS,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazin-2-carboxamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoropyridinamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyanopyridineamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyridineamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)pyridineamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazido[2,1-f][1,2]thiazido-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpyridineamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridineamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpyridineamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-chloropyridinamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-(difluoromethoxy)-pyridineamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2-difluoroethoxy)pyrazin-2-carboxamide,

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(prop-1-yn-1-yl)pyridineamide,

N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridineamide,

N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpyridineamide,

N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide,

N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-7-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyridineamide,

N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)pyridineamide,

N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridineamide,

N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpyridineamide,

N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazido[2,1-f][1,2]thiazido-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide,

N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-7-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyridineamide,

N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)pyridineamide,

N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazin-2-carboxamide,

N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridineamide,

N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpyridineamide,

N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide,

N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-7-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyridineamide,

N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)pyridineamide,

N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazin-2-carboxamide,

N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-3,5-dichloropyridineamide,

N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpyridineamide,

N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazido[2,1-f][1,2]thiazido-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide,

N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-7-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyridineamide,

N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)pyridineamide,

N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazido[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazido[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpyridineamide,

N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinamide,

N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpyridineamide,

N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)pyridineamide,

N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyridineamide,

N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)pyridineamide,

N-(6-((5aR,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazido[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazido[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpyridineamide,

N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinamide,

N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpyridineamide,

N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)pyridineamide,

N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyridineamide,

N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)pyridineamide,

N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazido[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazido[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpyridineamide,

N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinamide,

N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpyridineamide,

N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)pyridineamide,

N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazido[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyridineamide,

N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)pyridineamide,

N-(6-((5aS,6R,10R)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazido[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazido[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazido[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-fluoro-3-methylpyridineamide,

N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-3-chloro-5-fluoropyridinamide,

N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-chloro-3-methylpyridineamide,

N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazido[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-(fluoromethoxy)pyridineamide,

N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-(difluoromethoxy)pyridineamide,

N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)pyridineamide,

N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazido[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazine-2-carboxamide,

N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide,

N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-4,4a,5,8-tetrahydro-2H-spiro[[1,4]thiazino[2,1-f][1,2]thiazin-3,1′-cyclopropyl]-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-(difluoro-methoxy)pyridineamide,

N-(6-((3aS,4R,8R)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-4-chloro-1-(difluoromethyl)-1H-pyrazole-3-carboxamide, and

6-((6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)carbamoyl)nicotinic acid,

Or its medicinal salt.

20. The compound of formula I according to any one of claims 1-2, wherein the compound is selected from the group consisting of the following:

Or its medicinal salt.

21. The compound of formula I according to any one of claims 1-2, wherein the compound is selected from the group consisting of the following:

N-(6-((3aR,4R,8S)-6-amino-4,7,7-trimethyl-8-oxo-3,3a,4,7-tetrahydro-2H-isothiazo[1,5-a][1,4]thiazin-4-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((5aR,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazin-2-carboxamide, and

N-(6-((5aS,6R,10S)-8-amino-6,9,9-trimethyl-10-oxo-3,4,5,5a,6,9-hexahydro-2H-[1,4]thiazino[2,1-g][1,2]thiaza-6-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazin-2-carboxamide

Or its medicinal salt.

22. The compound of formula I according to any one of claims 1-2, wherein the compound is selected from the group consisting of the following:

N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazido[2,1-f][1,2]thiazido-5-yl)-5-fluoropyridin-2-yl)-3-chloro-5-cyanopyridineamide, N-(6-((4aR,5R,9R)-7-amino-3,3-difluoro-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazido[2,1-f][1,2]thiazido-5-yl)-5-fluoropyridin-2-yl)-5-methoxypyrazin-2-carboxamide,

N-(6-((4aR,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazinco[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-6-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aR,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazinco[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-6-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazinco[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-6-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

N-(6-((4aS,5R,9R)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazido[2,1-f][1,2]thiazido-5-yl)-5-fluoropyridin-2-yl)-5-(2,2,3,3-tetrafluoropropoxy)pyridineamide, N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazido[2,1-f][1,2]thiazido-5-yl)-5-fluoropyridin-2-yl)-5-cyano-3-methylpyridineamide,

N-(6-((4aS,5R,9S)-7-amino-5,8,8-trimethyl-9-oxo-2,3,4,4a,5,8-hexahydro-[1,4]thiazino[2,1-f][1,2]thiazin-5-yl)-5-fluoropyridin-2-yl)-6-chloro-3-methylimidazo[1,2-a]pyridine-2-carboxamide,

Or its medicinal salt.

23. The compound of formula I according to any one of claims 1-22, which is used as a therapeutically active substance.

24. The compound of formula I according to claims 1-22, which is used as a therapeutically active substance for the therapeutic and/or preventive treatment of diseases and conditions or Alzheimer's disease characterized by elevated β-amyloid levels and/or β-amyloid oligomers and/or β-amyloid plaques and further deposition.

25. A pharmaceutical composition comprising the compound of any one of claims 1-22.

26. Use of the compound of formula I according to claims 1-22 for the preparation of a medicament for the therapeutic and/or preventive treatment of diseases and conditions or Alzheimer's disease characterized by elevated levels of β-amyloid protein and/or β-amyloid oligomers and/or β-amyloid plaques and further deposition.

27. Use of the compound of formula I according to claims 1-22 for the preparation of a medicament for the therapeutic and/or preventive treatment of Alzheimer's disease.