HK1164699A - Composition comprising green tea extract - Google Patents

Description

Composition comprising green tea extract

Technical Field

The present invention relates to a composition comprising green tea extract as an active ingredient.

Background

With lifestyle changes and westernized eating habits, and adult diseases such as obesity, hyperlipidemia, hypertension and arteriosclerosis due to accumulation of body fat are becoming serious problems of modern people. Obesity is mainly due to high fat, high protein intake in the diet. Although obesity requires a diet that is moderate, it is not easy to exercise in daily life. Initial successful weight loss is often defeated by, for example, the yo-yo effect. Therefore, to maintain reduced body weight, prolonged effort and care is required.

For weight control, weight loss treatment for reducing body weight through adequate medication and healthy exercise is required. Weight loss therapy refers to the dietetic and controlled diet of obese or those who wish to maintain good physical fitness by reducing body weight. There is sometimes a tendency to resort to surgical means, such as liposuction or drugs, to achieve weight loss in a relatively short period of time, with side effects resulting therefrom.

Complications arise because most obese patients are treated after the cause. In addition, the therapeutic effects often vary depending on the age and sex of individuals, and the development of a weight loss mechanism ensuring the prevention and treatment of obesity is urgently required for modern people.

Disclosure of Invention

Technical problem

One embodiment of the present invention is to provide a composition comprising a first plucked green tea extract.

Another embodiment of the present invention is to provide a composition comprising green tea extract having a total catechin content of 20-40 wt%.

Technical scheme

The composition of one embodiment of the present invention comprises a first plucked green tea extract as an active ingredient. In one embodiment, the composition of the present invention comprises a green tea extract having a total catechin content of 20-40 wt% as an effective ingredient.

Advantageous effects

The composition of the present invention comprising green tea extract as an active ingredient has an effect on, for example, the treatment and prevention of obesity.

Drawings

FIG. 1 is a flow chart of tea catechin extraction, hot water extraction and alcohol extraction.

Fig. 2 shows the measurement results of the degradation ability of green tea extract in different concentrations of adipocytes.

Figure 3 shows the change in body weight according to the green tea extract taken.

Fig. 4 shows the final body weight of the green tea extract according to the administration.

Fig. 5 shows the epididymal fat weight in the average body weight of an individual according to the green tea extract administered.

Fig. 6 shows the toxicity test results of green tea extract in organs and tissues.

Best mode for carrying out the invention

The present invention provides a composition comprising green tea extract as an active ingredient. The extraction method of the green tea extract is not limited. In one embodiment, the extraction method may be by using hot water or C₁-C₅Is obtained by extracting the lower alcohol of (a). For example, green tea extract can be extracted by hot water extraction or ethanol extraction. In particular, the primary plucked green tea may be extracted with hot water. Hot water extraction of the primary plucked green tea may be obtained, for example, by the steps of fig. 1. In particular, the product can be obtained by adding green tea leaves, hot water extract, filtration, vacuum concentration and spray drying.

In one embodiment, the composition of the present invention comprises as an active ingredient a first plucked green tea extract. The primary plucked green tea extract is less bitter and sweet in taste because the primary plucked green tea has a higher amino acid content than the normal green tea when extracted by standard procedures. The amino acid is a theanine. The content of theanine having "deliciousness" was 2 times higher in the first plucked green tea than in the ordinary green tea without artificially increasing the theanine content. Also, the green tea extract from the primary digest has a considerably higher content of the important catechin, epigallocatechin gallate (EGCG), than the normal green tea extract.

The first plucked green tea extract of the present invention contains a large amount of components closely related to obesity, such as catechin, caffeine and theanine. Since these ingredients are naturally present, there is no artificial mixing, and they do not interfere with each other, but provide excellent effects as well as good taste in treating and preventing obesity.

As used herein, the term "first flush green tea" (first flush green tea) is also referred to as first flush tea, spring tea or head tea, and refers to green tea that is first picked every year. In korea, green tea is usually picked between april and may. Generally, green tea is initially picked manually to preserve its original characteristics as much as possible. Therefore, the yield is low and the price is expensive. In the present invention, the term "normal green tea" is used to distinguish it from "primary picked green tea". Ordinary green tea refers to green tea picked from May to autumn after the first picking of green tea.

The invention also provides a composition comprising a green tea extract having a total content of catechins in the range of 20 to 40 wt%, especially 25 to 35 wt%, of the total content of the extract. The catechin includes Epigallocatechin (EGC), Epicatechin (EC), epigallocatechin gallate (EGCG), epicatechin gallate (ECG), etc. In one embodiment, the green tea extract satisfying the above total content of catechins may be the primary cut green tea extract described above.

In adult diseases, obesity, accumulation of high toxins, oxidized low-density lipoprotein (oxy-LDL) in the form of cholesterol or cholesterol esters in the intima of blood vessels, causes the formation of foam cells, and causes arteriosclerosis. Tea catechins strongly inhibit the oxidation of Low Density Lipoprotein (LDL). Thus, a composition comprising the green tea extract or the first plucked green tea extract according to the present invention is a composition for treating and preventing arteriosclerosis.

Similarly, catechins have anti-obesity effects by lowering serum, liver cholesterol levels, inhibiting the reabsorption of cholesterol, and preventing histamine release from mast cells. Thus, a composition comprising a green tea extract or a first plucked green tea extract according to the present invention is a composition for preventing obesity.

In addition, catechin inhibits fat accumulation in the body by interrupting the activity of digestive enzymes in the small intestine, and thus prevents absorption and excretion of surplus nutrients. This is because catechin lowers blood insulin levels, thus lowering blood glucose and lowering body fat. Thus, the composition of the present invention comprising green tea extract or primary plucked green tea extract is a composition for treating or preventing obesity, hyperlipidemia or hypertension.

Another advantage of catechin is its detoxification activity against toxicity and damage due to drugs of abuse. Furthermore, catechin does not produce side effects even if it is digested for a long period of time in the form of, for example, tea leaves. This pharmacological activity results from the large number of hydroxyl (-OH) groups in catechins, which modify or inhibit these substances by simple bonding with other substances.

The green tea extract used in the present application has a relatively high total catechin content compared to the existing ordinary green tea extract. And particularly has EGCG, an important catechin, in an amount of 2 times that of the common green tea extract. In one embodiment, the green tea extract of the invention has an EGCG content of 7-20 wt%, in particular 10-15 wt%, based on the total weight of the extract.

In one embodiment, the green tea extract of the present invention has a caffeine content of 2.5-4.5 wt% based on the total weight of the extract. That is, the content of caffeine having superior decomposition ability is 1.5 times higher than that of the existing general green tea extract.

Among the derivatives of an alkaloid, methylxanthine, present in green tea, caffeine, a drug also used for vasodilation or psychotropic drugs, can stimulate the central nervous system and be selectively controlled by catechin and theanine, although its efficacy is not long lasting. In this regard, caffeine exhibits various pharmacological activities, including irritation, cardiac augmentation, diuresis, fever reduction, astringency, and the like. Particularly, it inhibits the increase of cholesterol in vivo by combining with polyphenols, and treats or prevents sore throat, cardiac infarction, etc. Therefore, the composition of the present invention comprising green tea extract or the extract of green tea from the initial harvest is a composition for treating or preventing angina pectoris, myocardial infarction.

In one embodiment, the green tea extract of the present invention further comprises amino acids in an amount of 4.5 to 10 wt% based on the total weight of the extract. In particular, theanine, which accounts for the majority of the amino acids of green tea, is difficult to find in other plants. Theanine is an important component determining the taste and efficacy of green tea and is reported to have different physiological effects. For example, theanine has received attention in various fields because it can control stimulation, relieve stress and stress, and improve immunity by caffeine. In one embodiment, the green tea extract according to the invention comprises theanine in an amount of 2-5 wt%, especially 2.5-3.5 wt% based on the total weight of the composition.

In the following examples, experiments were performed using hot water extracts of first plucked green tea plucked between forty-five months in jizhou island. The primary plucked green tea hot water extract was treated to culture different concentrations of adipocytes, and was studied for fat degradation efficacy by measurement of glycerol and free fatty acid growth. As a result, the hot water extract of green tea leaves from the primary harvest exhibited superior fat degradation effect compared to tea catechins (70%) used as a control.

Also, diet experiments were performed by using mice. Specifically, the mice were divided into the following groups for 8 weeks of experiment: a normal diet group, a high fat diet + tea catechins group, a high fat diet + primary-plucked green tea hot water extract (1/2 tea catechins) group, a high fat diet + primary-plucked green tea hot water extract (same amount as tea catechins) group, and a high fat diet + primary-plucked green tea hot water extract (2 volume times of tea catechins) group. As a result, the group of high fat diet + tea catechins did not show a particular weight loss effect, while the group of high fat diet + primary plucked green tea hot water extract (same amount or 2 times amount of tea catechins) showed a significant weight loss. Thus, it can be seen that the primary plucked green tea extract of the present invention is effective in reducing body weight.

In one embodiment, the present invention provides a food additive or functional health food comprising the green tea extract of the present invention.

The food additive or functional health food comprising green tea extract may be in various forms. For example, it can be processed into fermented milk, cheese, yogurt, fruit juice, probiotic products, food supplements, etc., and various food additives.

In one embodiment, the green tea extract may further comprise other ingredients within the scope of the present invention that do not negatively affect its primary efficacy. For example, further include additives such as perfumes, pigments, disinfectants, antioxidants, preservatives, humectants, thickeners, minerals, emulsifiers, synthetic polymers, etc. to improve physical properties. Further, for example: water soluble vitamins, oil soluble vitamins, polypeptide, polysaccharide, seaweed extract, etc. Those skilled in the art can select these ingredients without difficulty in consideration of an ideal formulation or purpose, and their additional contents can be selected within a range that does not adversely affect the purpose and efficacy of the present invention. For example, the above ingredients may be added in an amount of 0.01 to 5 wt%, especially 0.01 to 3 wt%, based on the total weight of the composition.

The extract according to the invention can be prepared in different formulations including solutions, emulsions, viscous mixtures, tablets, powders, etc., and can also be administered by different methods such as simple drinking, injection, spraying, squeezing, etc.

The invention also provides a pharmaceutical composition comprising green tea extract. The pharmaceutical composition of the present invention comprising the extract has the effects of controlling body weight, and reducing blood glucose and blood cholesterol.

When the extract of the present invention is used as a medicine, an organic or inorganic carrier is usually added to the extract as an effective ingredient to form a solid, semi-solid or liquid formulation for oral or parenteral administration.

Examples of formulations for oral administration include tablets, pills, granules, soft/hard capsules, powders, fine granules, powders, emulsions, syrups, pills, and the like. Examples of formulations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. The formulation of the active ingredient may be carried out simply by the methods generally employed and other suitable adjuvants generally employed, such as: surfactants, vehicles, colorants, fragrances, preservatives, stabilizers, buffers and suspending agents.

The pharmaceutical compositions of the present invention may be administered orally or parenterally, for example: rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal or subcutaneous administration.

The dose of the active ingredient to be administered depends on the age, sex and body weight of the user, the particular disease or pathological condition to be treated, the severity of the disease or pathological condition, the route of administration, and the discretion of the physician. The decision to administer a dose based on these parameters should be within the ability of one skilled in the art. The dosage is generally 0.001-2000 mg/kg/day, especially 0.5-1500 mg/kg/day.

Detailed Description

Example 1: hot water extraction of green tea extract

Green tea was first plucked, plucked in korea jizhou island. The primary plucked green tea was isolated and purified by hot water extraction. The detailed steps of hot water extraction are shown in figure 1.

In fig. 1, the flow chart on the left side shows a general process of extracting catechins from tea leaves, the flow chart on the middle part shows a process of extracting tea leaves with hot water, and the flow chart on the right side shows a process of extracting tea leaves with ethanol.

In this example, the primary plucked green tea is extracted by hot water extraction, which comprises adding five times the weight of a solvent (water) to the primary plucked green tea leaves, extracting with hot water at 50 to 80 ℃ for 0.5 to 12 hours, filtering, vacuum concentrating and spray drying.

Test example 1: analysis of the sample composition.

The composition of the first plucked green tea extract prepared in example 1 was investigated. In particular, the content of catechin, amino acids and caffeine was analyzed by the health & Functional Food Research Center (health & Functional Food Research Center). The results of this analysis are shown in tables 1, 2 and 3.

TABLE 1

Content (wt.) (%)	Common green tea	Green tea for initial picking	Tea catechins
				Histidine (His)	0.98	3.97	-
Aminocarbonylalanine (Asn)	4.16	5.10	0.58
				Serine (Ser)	0.85	1.59	0.03
Glutamine (Gln)	1.61	1.37	-
				Arginine (Arg)	0.80	3.03	8.70
Glycine (Gly)	0.36	0.42	-
				Aspartic acid (Asp)	3.90	4.42	0.03
Glutamic acid (Glu)	6.15	6.66	0.33
				Threonine (Thr)	0.45	0.67	-
Alanine (Ala)	0.64	0.61	-
				Proline (Pro)	0.29	0.25	0.01
Cysteine (Cys)	0.90	1.65	-
				Lysine (Lys)	0.32	0.32	-
Tyrosine (Tyr)	0.39	0.41	-
				Methionine (Met)	-	-	-
Valine (Val)	0.33	0.30	0.07
				Isoleucine (Ile)	0.28	0.24	-
Leucine (Leu)	0.23	0.27	0.08
				Phenylalanine (Phe)	0.39	0.41	0.06
Tryptophan (Trp)	0.35	0.50	-
				Theanine	1.89	2.97	0.00
Total amount of amino acids	4.23	6.19	0.99

TABLE 2

Content (wt.) (%)	Common green tea	Green tea for initial picking	Tea catechins
				Epigallocatechin (EGC)	8.82	8.33	12.21
Epicatechin (EC)	2.32	2.05	5.92
				Epigallocatechin gallate (EGCG)	6.39	11.36	38.47
Epicatechin gallate (ECG)	1.68	2.43	7.22
				Others	7.56	6.69	8.54
Total catechin content	26.77	30.86	72.38

TABLE 3

Content (wt.) (%)	Common green tea	Green tea for initial picking	Tea catechins
				Caffeine	2.12	2.81	0.41

The values in tables 1, 2 and 3 are the weight percent content per 1g of extract.

As shown in table 1, the first plucked green tea extract contained 6.19% total amino acids and 2.97% total theanine. In contrast, the ordinary green tea extract has a total content of 4.23% of amino acids and a total content of 1.89% of theanine. The green tea extract from the primary harvest had a total catechin content of 30.85% and a total epigallocatechin gallate (EGCG) content of 11.36%, while the common green tea extract had a total catechin content of 26.77% and a total EGCG content of 6.39%. Similarly, the green tea extract from the initial plucking had a caffeine content 25% higher than that of the normal green tea extract.

Therefore, the primary plucked green tea extract has a total amino acid content about 1.5 times higher than that of the general green tea extract. In particular, the theanine content is about 2 times higher. Similarly, the green tea extract from the first plucked tea has a total catechin content about 15% higher than that of the normal green tea extract, and the EGCG content is about 2 times higher. Furthermore, the green tea extract from the initial plucking had a higher content of caffeine compared to the normal green tea extract.

Test example 2: ability to degrade triglycerides in different adipocytes.

Step 1: culture and differentiation Induction of adipocytes

Mouse undifferentiated 3T3-L1 adipocytes (derived from 10% calf serum (Gibco Co., USA)) were cultured in Darbeck's modified eagle's medium (DMEM; Lonza, 12-604F, USA)Purchased at ATCC), they had 10% CO at 37 oC when the medium was replaced every other day₂The culture was performed in the incubator until 80% cell density was reached. Subsequently, after culturing for 48 hours in a medium containing 10% fetal bovine serum (Gibco co., USA), 0.5mM 3-isobutyl-1-methylxanthine (Sigma co., USA), 1 μ M dexamethasone (Sigma co., USA) and 167 nM insulin (Sigma co., USA), the medium was subsequently replaced with DMEM containing 10% fetal bovine serum and 167 nM insulin, and the cells were cultured for 48 hours. Finally, these cells were cultured in a medium containing only 10% fetal bovine serum for 48 hours to obtain differentiated adipocytes.

Step 2: treatment of differentiated adipocytes

After complete differentiation induction, adipocytes were detached from the medium, washed with PBS, and washed at 10% CO₂The culture chamber of (1) was cultured with low-glucose DMEM (1000 mg/L D-glucose without L-glutamine or phenol red; LM001-04, Welgene, Korea) containing 2% free fatty acid bovine serum albumin (Sigma Co., USA) for 24 hours. The low glucose DMEM was treated with tea catechins (70%, PFI co., japan) at a concentration of 50, 100 and 200 ppm as a positive control group, and treated with a hot water extract of ordinary green tea (BTC, korea) as a negative control group, or treated with a hot water extract of first plucked green tea from jizhou island (Bioland, korea) as a test group. Subsequently, at 10% CO₂The cells are cultured in the incubator.

And step 3: measurement of fat degradation ability after treatment of fully differentiated adipocytes

The cells cultured in step 2 were recovered from the medium and seeded in 50. mu.L microwell plates per well, and reacted with 50. mu.L of reaction mixture A of a free fatty acid meter (Roche, Cat # 1-383-175, Germany) at 25 ℃ for 10 minutes. After 5. mu.L of N-ethylmaleimide was added to each well, the primary absorbance was measured at 546 nm. Subsequently, 5. mu.L of reaction mixture B was added to each well and mixed well. After 15 minutes of reaction at 25 ℃, a measurement of the final absorption was carried out. The final free acid concentration relative to the blank concentration was determined from the difference between the final and initial absorption. The results are shown in table 2.

In FIG. 2, catechin did not have the ability to degrade fats at a concentration of 50 ppm compared to the control (medium). In contrast, the normal green tea extract and the first plucked green tea hot water extract showed the degradation ability of fatty acids. Also, at a concentration of 100 ppm, only the hot water extract of green tea from the first plucking exhibited fat degradation ability. At a concentration of 200 ppm, neither catechin, regular green tea nor first plucked green tea exhibited significant effects over the control group.

Test example 3: serum biochemical test of Diet Induced Obesity (DIO) model.

Step 1: preparation of samples

Tea catechins were passed daily (70%, Pharmafood inc., japan) dissolved in high pressure liquid chromatography grade (HPLC-grade) H before oral administration₂O (Sigma co., usa) was prepared as a control for animal experiments. Hot water extracts of green tea leaves from the initial pluck used in the test groups were also dissolved in HPLC grade H before oral administration₂O to give 100, 200 and 400mpk concentrations.

Step 2: determination of test population and effectiveness of body weight reduction and fat degradation

10 seven-year-old male C57BL/6J mice were prepared per group. After a one week dwell period, the mice were incubated at 12: the cages were kept in their own cages under a 12-hour light-dark cycle (light from 07:00 to 17: 00). These groups are: 1) normal diet group (normal feeding); 2) high fat diet group (control); 3) high fat diet + catechin 200mpk group (tea catechins); 4) high fat diet + primary green tea hot water extract 100mpk group (primary green tea 100 mpk); 5) high fat diet + primary green tea hot water extract 200mpk group (primary green tea 200mpk group), and 6) high fat diet + primary green tea hot water extract 400mpk group (primary green tea 400 mpk). The test substance is administered once daily for a fixed number of hours (10 a.m.) over 8 weeks. For 10 mice of the control group on a high fat diet, the same volume of water was administered.

Body weight measurements (at 11 a.m.) were made once a week. The final body weight of the test and control groups was measured at week 8 and the results are shown in figures 3 and 4.

Fig. 3 shows the change in body weight of each group, and fig. 4 shows the increase in body weight. In fig. 3 and 4, the body weight of the high fat diet + tea catechins group (tea catechins) increased from 19.25 ± 0.69 g to 33.33 ± 2.73 g after 8 weeks, with no significant weight loss effect compared to the control group. In contrast, the body weight of the first plucked green tea extract 200mpk group (first plucked green tea 200 mpk) increased from 19.12 ± 0.70 g to 31.59 ± 1.46 g after 8 weeks, while the body weight of the first plucked green tea 400mpk group (first plucked green tea 400 mpk) increased from 19.24 ± 0.68 g to 30.50 ± 2.50 g after 8 weeks. That is, the primary plucked green tea extract according to the present invention has a statistically significant effect of inhibiting weight gain.

When the epididymal fat weight was measured at week 8, the control group had an epididymal fat weight of 2.102 + -0.170 g, while the green tea extract from the initial plucking 200mpk group (initial plucking green tea 200 mpk) had an epididymal fat weight of 1.862 + -0.099 g and the green tea extract from the initial plucking 400mpk group (initial plucking green tea 400 mpk) had an epididymal fat weight of 1.543 + -0.069 g. The measurement results were calculated from the average body weight of each person. The results are shown in fig. 5.

In fig. 5, the high fat diet + primary plucked green tea hot water extract 400mpk (primary plucked green tea 400 mpk) showed a statistically significantly lower epididymal fat weight compared to the other groups. Therefore, the first plucked green tea extract of the present invention has the efficacy of fat degradation.

And step 3: serum test of test group and control group

Organ toxicity tests were performed on C57BL/6J mice given various concentrations of tea catechins and the primary green tea extract at 8 weeks.

To test the efficacy on animal organs (tissues), blood was drawn from animals given the group that took tea catechins and the primary plucked green tea hot water extract for 8 weeks.

In fig. 6, High Density Lipoprotein Concentration (HDLC) and Low Density Lipoprotein Concentration (LDLC) are used as indicators to indicate that obesity is generally reduced. Of all the control groups, the tea catechins and the primary plucked green tea group (primary plucked green tea 100 mpk), primary plucked green tea 200mpk and primary plucked green tea 400 mpk) exhibited similar HDLC and LDLC values. Therefore, it was confirmed that obesity was generally reduced in all the high fat diet groups (control group, tea catechins group, primary plucked green tea 100mpk group, primary plucked green tea 200mpk group, and primary plucked green tea 400mpk group) except the normal diet group (normal feeding).

Glutamate Pyruvate Transaminase (GPT) is an indicator of hepatotoxicity, whereas BUN is an indicator of nephrotoxicity. No significant difference was shown in the test group (catechin group, first plucked green tea 100mpk, first plucked green tea 200mpk and first plucked green tea 400 mpk) from the control group. Similarly, when the liver and kidney were removed from the body of the animal and tissue observation was performed with the subsequent tissue section, no particular abnormality was found. Therefore, the primary plucked green tea extract according to the present invention has no particular toxicity.

Glucose (GLUC) is an indicator of blood glucose. High glucose levels are often associated with obesity. Triglyceride (TG) is a blood lipid component that, together with cholesterol, causes arteriosclerosis. Also, Cholesterol (CHOL) is a diagnostic indicator of obesity, liver disease and diabetes. As shown in fig. 6, the primary plucked green tea group (primary plucked green tea 100mpk, primary plucked green tea 200mpk, primary plucked green tea 400 mpk) exhibited lower GLUC and CHOL levels in a concentration-dependent manner compared to the control group. Therefore, the composition of the present invention is effectively used for the treatment of diabetes and obesity.

Also, as shown in fig. 6, the primary plucked green tea groups (primary plucked green tea 100mpk, primary plucked green tea 200mpk, and primary plucked green tea 400 mpk) exhibited superior lower TG levels in a concentration-dependent manner compared to the control group. Therefore, the composition of the present invention has the effect of reducing the levels of CHOL and TG, and thus is effectively used for the treatment and prevention of hyperlipidemia, hypertension, arteriosclerosis, angina pectoris and myocardial infarction.

The compositions of the present invention comprising the primary plucked green tea extract may be prepared using the following different formulations, but are not limited to these formulations.

Formulation example 1: soft capsule

Soft capsules can be prepared by a common method by mixing 100 mg of the extract of green tea of primary harvest with 50 mg of soybean juice, 180 mg of soybean oil, 50 mg of red ginseng extract, 2 mg of palm oil, 8 mg of hydrogenated palm oil, 4 mg of yellow beeswax and 6 mg of lecithin, and filling 400 mg of the mixture in each capsule.

Formulation example 2: tablet(s)

100 mg of the extract of green tea from the first crop was mixed with 50 mg of soybean juice, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate. After addition of 40 mg of 30% ethanol, the resulting granules were dried at 60 ℃ and tableted.

Formulation example 3: granules

100 mg of the first plucked green tea was mixed with 50 mg of soybean juice, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch. After addition of 100 mg of 30% ethanol, the resulting granules were dried at 60 ℃ and filled into sachets. The final weight was 1g per bag.

Formulation example 4: beverage product

100 mg of the first plucked green tea extract was mixed with 50 mg of soybean juice, 10 g of glucose, 50 mg of red ginseng extract, 2 g of citric acid and 187.8 g of purified water, and bottled. The final volume was 200 mL per bottle.

Formulation example 5: health food

Green tea extract 1000 mg

Vitamin mixture

Vitamin A acetate 70 μ g

Vitamin E1.0 mg

Vitamin B₁ 0.13 mg

Vitamin B₂ 0.15 mg

Vitamin B₆ 0.5 mg

Vitamin B₁₂ 0.2 μg

Vitamin C10 mg

Biotin 10. mu.g

Nicotinamide 1.7 mg

Folic acid 50 ug

Calcium pantothenate 0.5 mg

Mineral mixture

Ferrous sulfate 1.75 mg

Zinc oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium dihydrogen phosphate 15 mg

Calcium Hydrogen phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

24.8 mg of magnesium chloride

The vitamin and mineral mixture described above is an example of a suitable health food. The composition may be varied. The above ingredients may be mixed to prepare granules as health foods according to a general method used.

Formulation example 6: health beverage

Green tea extract 1000 mg

Citric acid 1000 mg

Oligosaccharide 100 g

Plum concentrate 2 g

Taurine 1g

Preparation of purified water 900 mL

The above ingredients were heated with stirring at 85 ℃ for 1 hour according to a commonly employed method. The resulting solution was filtered, placed in a sterile 2-L container, sealed, sterilized, and stored in a refrigerator as a healthy drink.

The composition described above is one example suitable for use in a favorite beverage. The composition may vary depending on the age, nationality, purpose of use, regional or ethnic preference of the consumer, etc.

Those skilled in the art will appreciate that the conceptions and embodiments disclosed in the foregoing description may be readily utilized as a basis for modifying or designing other embodiments for carrying out the same purposes of the present invention.

Industrial applications

The composition of the present invention comprising green tea extract as an active ingredient can be widely used in, for example, food and pharmaceutical fields.

Claims (20)

1. An anti-obesity composition comprising a first plucked green tea extract as an active ingredient.

2. A composition for treating or preventing diabetes, comprising an extract of green tea from primary plucking as an active ingredient.

3. A composition for treating or preventing hyperlipidemia, comprising an extract of green tea from primary plucking as an active ingredient.

4. A composition for treating or preventing hypertension, comprising an extract of green tea originally plucked as an active ingredient.

5. A composition for treating or preventing arteriosclerosis, comprising an extract of green tea from primary plucking as an active ingredient.

6. A composition for treating or preventing angina pectoris, characterized by comprising an extract of green tea initially plucked as an active ingredient.

7. A composition for treating or preventing myocardial infarction, comprising an extract of green tea of primary plucking as an active ingredient.

8. An anti-obesity composition comprising a green tea extract as an active ingredient, wherein the total content of catechins in the green tea extract is 20 to 40 wt% based on the total weight of the extract.

9. Composition for treating or preventing diabetes, characterized by comprising green tea extract as an active ingredient, wherein the total content of catechins in the green tea extract is 20 to 40 wt% based on the total weight of the extract.

10. Composition for treating or preventing hyperlipidemia, comprising green tea extract as an active ingredient, wherein the total content of catechins in the green tea extract is 20 to 40 wt% based on the total weight of the extract.

11. Composition for treating or preventing hypertension, comprising green tea extract as an active ingredient, wherein the total content of catechins in the green tea extract is 20 to 40 wt% based on the total weight of the extract.

12. Composition for treating or preventing arteriosclerosis, characterized by comprising a green tea extract as an active ingredient, wherein the total content of catechins in the green tea extract is 20 to 40 wt% based on the total weight of the extract.

13. A composition for treating or preventing angina pectoris, characterized by comprising a green tea extract as an active ingredient, wherein the total content of catechins in the green tea extract is 20 to 40 wt% based on the total weight of the extract.

14. Composition for treating or preventing myocardial infarction, characterized by comprising green tea extract as an active ingredient, the total content of catechins in the green tea extract being 20-40 wt% based on the total weight of the extract.

15. The composition of any one of claims 1-14, wherein the first plucked green tea extract or the green tea extract is a hot water extract or C₁-C₅A lower alcohol extract.

16. The composition of claim 15, wherein C is₁-C₅The lower alcohol extract is ethanol extract.

17. The composition according to any one of claims 8 to 14, wherein the catechin in the green tea extract comprises epigallocatechin gallate, the total content of epigallocatechin gallate being 7 to 20 wt% based on the total weight of the extract.

18. The composition according to claim 17, wherein the green tea extract comprises caffeine in an amount of 2.5 to 4.5 wt% based on the total weight of the green tea extract.

19. The composition of claim 17, wherein the total content of amino acids in the green tea extract is 4.5-10 wt% based on the total weight of the extract.

20. The composition of claim 19, wherein the amino acids comprise theanine in an amount of 2 to 5 wt% based on the total weight of the extract.