HK1156468B - Versatile disinfectant - Google Patents

Description

Universal sterilizing disinfectant

Technical Field

The present invention relates to a novel disinfectant solution which is not known in the prior art, has a high safety and a wide antimicrobial spectrum, contains amino acids, vitamins and minerals as main components, which satisfy the basic substances of life and life activities, can disinfect fingers, mucous membranes, wounds, instruments, equipment, excretions, the environment, and further, fresh edible materials and crops, and can be used for various purposes and uses.

Background

The history of human fight against pathogenic bacteria has begun at the moment when humans are born, and since then for a long time humans have not had effective weapons for their enemies who are invisible to the eyes, and have been only frustrated once and feared.

Meanwhile, the evolution from hunting to the farming society has prompted people to live at a stable position and to have an increased population density, resulting in the occurrence of large-scale infections caused by exposure to droplets. With the development of transportation means such as silk roads, big navigation, roads crossing africa and the like, the characteristic infection diseases such as plague, cholera, syphilis, malaria and the like are spread. For example, smallpox occurs in ancient india, china, and thereafter spreads to the middle east, egypt, and invades europe with the cross military, crossing to the continental america through discovery of the new continent. Syphilis is introduced into europe from america in the reverse route.

Beginning with the discovery of anthrax by koch in 1876, it was known that the cause of these infections afflicting humans is microorganisms, and since then, infections and their pathogens became increasingly clear, and since then, the official struggle between humans and pathogens began.

Human wisdom thought about the method of attacking pathogens, pursuing them with effective disinfectant development and the advent of antibiotics and vaccines, temporarily seen the potential for a complete victory. However, it is understood that this is only a momentary illusion. Bacteria and viruses that have been able to withstand and adapt to the great changes in the environment to expand the range of activities, since the generation of microorganisms on the earth three billion years ago, should not simply fail as such.

Abuse of antibiotics resulted in non-drug resistant methicillin-resistant staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), multidrug-resistant pseudomonas aeruginosa (MDRP), and multidrug-resistant tubercle bacillus (XMR-TB). The development of economically preferential confusion causes Ebola hemorrhagic fever, West Nile fever, Lassa fever, macula fever, Nipah virus encephalitis, and the like, which are endemic diseases of wild animals. Since then, unknown infections (new infections) that can span species barriers, both the route of infection and the cause ascertainment are not progressing will necessarily continue to emerge. In recent cases, for example, in highly pathogenic avian influenza, the virus is slightly mutated, and it is possible that infection between humans becomes a real problem (new influenza occurs), and the new influenza spreads worldwide in a very short time (influenza pandemic), and the number of deaths is probably less than 1 hundred million people.

Analysis suggests that pathogens change the way of attack on humans in response to social changes such as our lifestyle and environmental changes, medical advances, internationalization, and aging. The world has in fact appeared to be vulnerable to surviving pathogens that mutate at rates many tens of millions of times greater than humans.

One of the means to protect itself from such a directly invisible terrorist pathogen is presumably to achieve a global scale population of public health systems and establish crisis monitoring regimes, provide intelligence quickly and, although there may be objections, thoroughly disinfect the pathogen.

Conventionally, there are general-purpose disinfectants such as alcohols, phenols, halides, quaternary ammonium salts, biguanide agents, and aldehydes, and recently, strongly acidic water and ozone water have also been used for sterilization of fresh food materials.

(1) Broad antimicrobial spectrum

(2) Can be sterilized by short contact time (quick action)

(3) High stability and continuous bactericidal action

(4) Little decrease in efficacy even in the presence of organic substances

(5) Has little harm to human body (low toxicity)

(6) Easy to dissolve in water, and simple application method

(7) High permeability

(8) Has no unpleasant odor

(9) Is cheap

(10) Has good storability

(11) Is easy to be discarded

(12) Without damaging the object to be used, and further

(13) Drug-resistant bacteria are not formed.

However, as can be seen in the case of bacterial and antibiotic relationships, bacteria will sooner or later acquire resistance to agents (compounds) that attack themselves. This is an irrefutable fact, reporting examples of nosocomial infections with bacteria that are ineffective with various alcohols and quaternary ammonium or biguanide agents.

In addition, unfortunately, a germicidal sterilant satisfying all of the above conditions (1) to (13) does not exist. Therefore, the existing disinfectants are currently selected depending on the purpose of use to determine which of these conditions is most important.

Initially, with modern medicine dawn starting from the middle of the 19 th century, people were encumbered with the eye being closed to many phytotoxicity and toxicities, and the fact that medical development and pursuit of truth were not contended with the sacrifice of the public was achieved.

Since the 21 st century, the world of society and the line of sight of the masses have evolved into an era where "reassurance and safety" are considered to be more important than ever and are placed in top priority.

Taking these current situations into account, the inventors of the present invention developed a sterilizing solution that substantially satisfies the above-mentioned requirements, and filed and patented as "a sterilizing solution containing iron ions" in 1998 (patent document 1).

The bactericidal solution is composed of several compounds approved for use even as food additives, and although it is low in toxicity, it suppresses the appearance of resistant bacteria in the case of tubercle bacillus and helicobacter pylori eradication by the same principle as in the case of using a plurality of drugs in combination. In addition, the antibacterial agent has the excellent characteristics of wide antibacterial spectrum, effectiveness to spores and the like. However, the inventors of the present invention have further studied the above-mentioned iron ion-containing bactericidal liquid, and have found that the main component of the bactericidal liquid is Fe³⁺The influence of ions is not 100% satisfactory for the user in terms of gradual coloring (when left standing) of the bactericidal liquid and odor of the bactericidal liquid, whether the bactericidal liquid itself or a control is used, and also partial damage to the growth of the crops when spread for the purpose of disinfection, and the like. The above-mentioned bactericidal solution containing iron ions is excellent in bactericidal activity against various pathogenic bacteria, but unsatisfactory in bactericidal activity against viruses.

Patent document 1: japanese patent publication No. 3853985

Disclosure of Invention

The present invention has been made in view of the above circumstances, and an object thereof is to provide a disinfectant solution which is almost nontoxic and harmless to humans and animals, shows a high bactericidal activity against a wide range of pathogenic microorganisms, and has an excellent persistence of a bactericidal action, and a method for sterilization using the disinfectant solution.

The inventors of the present invention have made intensive studies on the development of a sterilizing liquid having lower toxicity and no adverse side effects while overcoming the problems described above and the difficulties in obtaining drug resistance of the sterilizing liquid described in patent document 1 which the inventors of the present invention previously developed, and have made a change of directions to investigate the specifications of the sterilizing liquid in detail. As a result, it has been found that when the concentration of a substance is on the boundary line between "positive" and "negative" for the development of microorganisms, a specific disturbance phenomenon is exhibited to the microorganisms, and the phenomenon is captured, and if the substance is induced to the "negative" side, the microorganisms are stabilized, and the inhibition occurs first, and if the inhibition is increased, the sterilization is achieved.

Based on these findings, the present inventors have succeeded in developing an ideal universal disinfectant that is almost non-toxic to humans, animals and plants, exhibits high bactericidal activity against a wide range of pathogenic microorganisms due to its synergistic effect, and can be used for almost all subjects, by using amino acids, vitamins, and minerals that have high affinity for the body among basic substances of life as main components, and combining a small amount of surfactants and the like that are safe for long-term use in daily necessities.

That is, the present invention relates to the following inventions:

[1] a disinfecting solution characterized by comprising one or more metal ions having an antibacterial action, L-cysteine and L-ascorbic acid as main components, and a surfactant other than a nonionic surfactant as a main component.

[2]As described above [1]The disinfectant according to (1), wherein the metal ion having antibacterial activity is a trivalent iron ion (Fe)³⁺) Divalent iron ion (Fe)²⁺) Zinc ion (Zn)²⁺) Copper ion (Cu)²⁺) Cobalt ion (Co)²⁺) Nickel ion (Ni)²⁺) Or silver ion (Ag)⁺)。

[3] The disinfectant as described in the above [2], wherein the concentration of the metal ion having antibacterial activity is 50 to 200ppm in the case of trivalent iron ion, 110 to 400ppm in the case of divalent iron ion, 7.5 to 125ppm in the case of zinc ion, 15 to 60ppm in the case of copper ion, 180 to 300ppm in the case of cobalt ion, 85 to 175ppm in the case of nickel ion, and 1 to 3ppm in the case of silver ion.

[4] The disinfectant according to any one of the above [1] to [3], wherein the concentration of L-cysteine is 100 to 1000ppm and the concentration of L-ascorbic acid is 100 to 500 ppm.

[5] The disinfectant according to any one of [1] to [4], wherein the surfactant other than the nonionic surfactant is at least one selected from the group consisting of alkylbenzenesulfonates, linear alkylbenzenesulfonates, polyoxyethylene alkyl ether sulfates, higher alcohol sulfates, sodium lauryl sulfate, sodium lauroyl sarcosinate, octadecyl dimethyl benzyl ammonium chloride, benzalkonium chloride, benzethonium chloride, alkyl diamino ethyl glycine hydrochloride and alkyl poly amino ethyl glycine hydrochloride.

[6] The disinfectant according to any one of the above [1] to [5], wherein the concentration of the surfactant other than the nonionic surfactant is 20 to 100 ppm.

[7] The disinfectant liquid according to any one of the above [1] to [6], further comprising at least one selected from sorbic acid, sorbate, benzoic acid, benzoate, and paraben.

[8] The disinfectant solution according to [7] above, wherein the concentration of sorbic acid, sorbate, benzoic acid, benzoate, and paraben is 50 to 100 ppm.

[9] The disinfectant according to any one of the above [1] to [8], wherein the pH is adjusted to 2.5 to 4.0.

[10] A sterilization method characterized by bringing the sterilizing liquid according to any one of the above [1] to [9] into contact with an object to be treated.

[11] The sterilization method according to [10], wherein the object to be treated is an environment, an appliance, a human body, an animal body, a plant body or an organic matter.

ADVANTAGEOUS EFFECTS OF INVENTION

The disinfectant of the present invention is extremely safe because it contains, as main components, amino acids, vitamins and minerals having high affinity for the body, which are the sources of life, and can strongly disinfect and disinfect various pathogens including general bacteria, acid-fast bacteria, spores, fungi and viruses by introducing a concept which has not been found so far to maximize the microscopic action of minerals.

The disinfectant of the present invention is not particularly limited, and is effective for direct use on skin and mucous membranes, as well as excrement, instruments, equipment, and the environment. They are also widely used as external medicines such as medical toothpastes, medical soaps, and mouth washes, as cosmetics in shampoos and hair dyes, and in hygiene and deodorization for pets.

In addition, the sterilizing and disinfecting liquid of the invention has high utilization value in purification of fresh food such as vegetables and fish, and auxiliary aspect of treatment of infectious diseases of human and animals.

Drawings

FIG. 1 is a schematic view showing concentrations at which a sterilizing action is sufficiently exhibited in a range from a concentration at which a bacterial growth inhibitory action is clearly exhibited to a concentration at which a bacteriostatic action is clearly exhibited.

FIG. 2 is a schematic explanatory view showing the change in the antibacterial action intensity when a metal ion and a food preservative are used in combination.

Detailed Description

[1] In order to develop the disinfectant solution of the present invention, the respective components used in patent document 1 were investigated again.

(1) Re-study of Metal ions (verification and study of mineral Components)

Minerals are one of 5 macronutrients, and function as the core of enzymes that play a role in the organism as the root of various physiological actions or as catalysts.

On the other hand, antibacterial properties of silver and copper have been known empirically since ancient times and have been used in daily life such as tableware, currency, and ornaments, but the organic disinfectant as an antibacterial agent has a bright phase, and there is almost no current and retrospective evidence other than some products. Under such circumstances, the inventors of the present invention employed ferric ion (Fe) which has relatively low toxicity, can be used as a food additive, and exhibits antibacterial activity, in the disinfectant solution of patent document 1³⁺) As the main component.

Such inorganic antimicrobial agents are all effective in a short time, but have the characteristics of broad basic antimicrobial spectrum, and excellent stability and storage stability. Not limited to the above Fe³⁺Ions, from among minerals essential for ingestion into body tissues, to minerals exhibiting an antibacterial action in trace amounts: (Microscopic action of metal) was tried and investigated, and as a result, zinc ion (Zn) was selected²⁺) Copper ion (Cu)²⁺) Cobalt ion (Co)²⁺) Nickel ion (Ni)²⁺) Divalent iron ion (Fe)²⁺) And silver ion (Ag) having practical performances since ancient times⁺) The correlation between the ion concentration and the antibacterial activity of these minerals and the like was precisely examined, namely, the precise examination was carried out in 3 stages classified into a) a bacterial growth inhibitory action, b) a bacteriostatic action and c) a bactericidal action.

In addition, in data and the like in the present specification, which will be described later, the term "mineral ions" is collectively referred to as a common term "metal ions".

1) Addition of various metal ions to the growth medium

In a standard viable cell count measuring medium shown below, each metal ion was added at varying concentrations, followed by inoculation of a suspension (1X 10) of 1 wt% of the test cell⁹Cells/ml raw drinking water), the number of viable bacteria was measured over time according to a conventional method, and the correlation between each metal ion and the antibacterial activity was observed according to the growth and decay thereof, and summarized in table 1.

Test bacteria: representative of gram-positive bacteria S.aureus209P

: coli O-157 as a gram-negative bacterium

Standard viable cell count measurement medium: yeast extract 2.5g, peptone 5g, glucose 1g, agar 15g, pH7.2

[ Table 1]

TABLE 1 antibacterial Activity of Metal ions (1)

For s.aureus209p

Watch 1 (2)

For E.coli O-157

When the metal ions were added to the medium to culture bacteria, it was found that the boundary between the proliferation inhibitory effect and the bacteriostatic effect due to the metal ion concentration was not clear, but the bacteriostatic effect and the bactericidal effect could be roughly clearly distinguished.

For example, in Cu²⁺In the case of (2), the compound exhibits a growth inhibitory action in the range of 10 to 50ppm, a bacteriostatic action in the range of 40 to 150ppm, and a bactericidal action if the compound is 150ppm or more.

In addition, in the above table only describes various metal ion representative compounds, but for water soluble to become ionic other compounds, for example, with Fe³⁺Related ferric chloride, ferric nitrate hexahydrate, ferric nitrate nonahydrate, ferric nitrate n-hydrate, ferric phosphate n-hydrate, ferric citrate n-hydrate, etc., with Fe²⁺Related ferrous chloride tetrahydrate, ferrous gluconate, ferrous citrate, ferrous oxalate, etc., with Zn²⁺Related zinc citrate dihydrate, zinc gluconate, etc., with Cu²⁺Related copper chloride dihydrate, copper diammonium chloride dihydrate, copper nitrate trihydrate and the like, with Co²⁺Associated cobalt gluconate trihydrate, cobalt hydroxide, cobalt citrate, etc., with Ni²⁺Related nickel nitrate etc. and Ag⁺The related silver sulfate, silver phosphate, etc. also showed similar results.

Incidentally, the moderate presence of metal ions (minerals) shows promotion of development. (2) Based on the results of tables (1) and (2) in Table 1, 1 wt% of the test cell suspension (1X 10) was added to the aqueous solution (purified water 28 ℃ C.) containing each metal ion dissolved therein⁹Cells/1 ml water), which are then inoculatedOn the other hand, 0.2ml of the antimicrobial agent was collected every time, and the relative relationship between the metal ion concentration and the antimicrobial activity was estimated by immediately measuring the viable cell count by a conventional method and measuring the mortality. In addition, as the test bacteria, Staphylococcus aureus209P and Escherichia coli O-157 were used, and since these 2 strains showed similar results, the average values thereof are shown in the following Table 2.

[ Table 2]

TABLE 2 antibacterial Activity of Metal ions in purified Water

In the purified water containing no metal ion, the mortality rate was 2% in 60 minutes and about 3% in 120 minutes (autolysis), and in the purified water containing metal ion, the ion concentration thereof was proportional to the mortality rate, but it was found that the difference in the mortality rate due to concentration shading was small when the purified water was contacted for a short time of about 10 to 15 minutes, and even if the concentration was extremely low, the action was not sharp, but the bacteria were gradually killed at an accelerated rate if the contact time was prolonged.

From the test data relating to metal ions in the above tables 1 and 2, it can be presumed that:

<1> if the proliferation of bacteria is inhibited by adding metal ions to the medium, the route of bactericidal action is sustained at this concentration.

<2> when the sterilization by the short contact time is expected, the concentration is sufficient from the concentration at which the bacterial growth inhibition effect is clearly exhibited to the concentration at which the bacteriostatic effect is clearly exhibited (hatched portion in fig. 1).

That is, even if bacteria are brought into contact at a concentration at which the bacteria exhibit bactericidal activity, a very high killing ability cannot be expected.

Therefore, it is reasonable to consider safety, and to make up for the insufficiency of the concentration in the concentration range shown in <2>, thereby maximizing the sterilization effect.

(2) Validation and researcher of food preservatives

Then, in patent document 1, Fe is ensured for strengthening³⁺The sterilizing effect of the ions, and food preservative.

Then, the inventors of the present invention performed a precise inspection in this regard.

The food preservatives such as sorbic acid, sorbate, benzoic acid, benzoate and p-hydroxybenzoate are lack of bactericidal activity and play a role in bacteriostasis. Inhibit the action of microbial dehydrogenases and prevent their development.

1) When various food preservatives are added to the culture medium for proliferation

The same experiment was continued with the above metal ions, and the following table 3 summarizes the correlation between the concentration of the food preservative and a) the inhibitory effect on bacterial growth and b) the inhibitory effect on bacterial growth.

[ Table 3] antibacterial Activity of food preservative

As is clear from the above table, there is no significant difference in the antibacterial activity according to the type of food preservative, the proliferation inhibitory effect is generally exhibited at a concentration of 20 to 300ppm, the bacteriostatic effect is exhibited at a concentration of 200 to 300ppm or more, and the boundary between the two effects is not obvious and overlaps.

2) Dissolving food preservative in refined water

Based on the results in table 3, the same tests as in the case of the metal ions were performed. In addition, since the effect is increased on the acidic side, the pH is adjusted to pH 3.0.

[ Table 4] mortality with time due to food preservatives

When a food preservative is added to purified water, the mortality rate is higher than that of purified water to which no food preservative is added, but it may be caused by promotion of "autolysis". That is, unlike the metal ions described above, no bactericidal effect is exhibited. There was no difference in the mortality rate due to the concentration, and the difference was slight even when 500ppm, which was 50ppm10 times, was added. Therefore, it is not meaningful to suggest that the preservative is used at a high concentration for the purpose of preserving food.

Next, referring back to patent document 1, it is attempted to verify the effect of the combination of both, that is, the metal ion and the food preservative

Although the test amounts of 7 kinds of metal ions having different concentrations and 5 kinds of food preservatives having different concentrations were enormous, it was concluded that when a food preservative was added to metal ion water having a concentration that spanned the growth inhibitory effect and the bacteriostatic effect, if the amount of the food preservative added was 200ppm or more, Fe was excluded as an iron ion³⁺、Fe²⁺In addition, the bactericidal effect of other metal ions is weakened, and the addition of a small amount (50 to 100ppm) produces an additive effect (see FIG. 2).

(3) Validation of L-ascorbic acid

L-ascorbic acid is discussed next.

L-ascorbic acid (vitamin C) is essential in the body, is very compatible with body tissues, and increases the affinity for cell tissues with a small addition amount. The inventors of the present invention have found, after their studies, that an aqueous solution of L-ascorbic acid itself has almost no antibacterial activity, but contributes to stabilization and maintenance and continuation of activity of metal ions and a food preservative with a strong antioxidant activity, and at the same time, increases the antibacterial activity of metal ions when it comes into contact with organic substances such as bacteria, and causes strong damage to pathogens.

However, it is also known that when the amount of L-ascorbic acid added is large, the antibacterial effect is rather reduced.

For example, when 500ppm or more of Fe is used³⁺In the case of (2), a concentration of 500 to 2000ppm is recommended, but for the purpose of sufficiently extracting the microscopic action of the metal ion of the present invention, a concentration of 100 to 500ppm is preferable, and it has been confirmed that L-ascorbic acid is not essential by the use and addition amount of L-cysteine, a surfactant and the like described later.

As described above, the main constituent components of the invention described in patent document 1 were verified and studied again, and as a result, Fe was found to be a core material thereof³⁺The amount of the ion content is clearly evident from coloring and taste, odor or damage to plants.

It was also confirmed that the addition of a food preservative as an enhancer in an amount of not less than a certain amount further promotes coloring (oxidation reaction).

Addition of a large amount of L-ascorbic acid, which is responsible for suppressing the oxidation reaction and enhancing the sterilization, is to be avoided because the sterilizing power is rather lowered.

Namely, Fe is known³⁺The amount and concentration of the first constituent components cause the above problems.

Therefore, intensive studies have been made to solve these problems at once, and as a result, not only Fe but also Fe is used in this case³⁺And Fe with very little effect²⁺、Zn²⁺、Cu²⁺、Co²⁺、Ni²⁺、Ag⁺The food preservative has minimized usage amount of each ion in consideration of safety to animals, plants and environment, and maximally elicits its effectWhen the metal ion concentration is low, the addition effect is small, and therefore, the metal ion is not used (when used, the amount is small).

Further, since the amount of the above-mentioned component added is small, it is sufficient to add L-ascorbic acid in a small amount, and therefore, in addition to this, (A) a compound contributing to the enhancement of bactericidal activity is searched for in addition to the metal ion component having the above-mentioned antibacterial action, and (B) a method of rapidly permeating the interior of a pathogen is sought. That is, it is considered that the combination of the above (a) and (B) can extract the minimal action of the metal ion having the antibacterial action to the utmost.

As a result, several candidates satisfying the above (A) and (B) were selected through careful experiments, and finally, as a combined use product used in combination with the above (A) and (B), a surfactant other than a nonionic surfactant (an anionic surfactant, a cationic surfactant, and an amphoteric surfactant) and L-cysteine were used, and then, as (B), the osmotic pressure was increased, and the development of the disinfectant of the present invention, which satisfies the official designation of "universal" and has high safety, a broad antimicrobial spectrum, and convenience, was successful.

The disinfecting and sterilizing liquid of the present invention will be described below.

[ II ] the sterilizing and disinfecting liquid of the present invention

The disinfecting and sterilizing liquid of the present invention is characterized by comprising metal ions having an antibacterial action, L-cysteine and L-ascorbic acid as main components, and a surfactant other than a nonionic surfactant as a main component.

(1) Metal ions with antibacterial effect

The metal ion having an antibacterial action may be as described in [ I ] above]As the various metal ions verified in the above, trivalent iron ion (Fe) can be cited³⁺) Divalent iron ion (Fe)²⁺) Zinc ion (Zn)²⁺) Copper ion (Cu)²⁺) Cobalt ion (Co)²⁺) Nickel ion (Ni)²⁺) Or silver ionSeed (Ag)⁺). These metal ions may be used alone or in combination.

The amount of the metal ion in the disinfectant solution of the present invention can be suitably adjusted to obtain a desired bactericidal activity, and is preferably 50 to 200ppm in the case of iron ion, 110 to 400ppm in the case of divalent iron ion, 7.5 to 125ppm in the case of zinc ion, 15 to 60ppm in the case of copper ion, 180 to 300ppm in the case of cobalt ion, 85 to 175ppm in the case of nickel ion, and 1 to 3ppm in the case of silver ion, for example.

As the metal ion, for example, the various compounds described above which are soluble in water and become ions can be used. For example, for Fe³⁺Examples of the ion include ferric chloride, ferric nitrate hexahydrate, ferric nitrate nonahydrate, ferric nitrate n-hydrate, ferric phosphate n-hydrate, and ferric citrate n-hydrate, and in the case of Fe²⁺Examples of the ion include ferric chloride tetrahydrate, ferric gluconate, ferric citrate, and ferric oxalate, and Zn is present²⁺Examples thereof include zinc citrate dihydrate and zinc gluconate, and Cu²⁺Examples thereof include copper chloride dihydrate, diammonium copper chloride dihydrate and copper nitrate trihydrate, and Co is used²⁺Examples thereof include cobalt gluconate trihydrate, cobalt hydroxide, and cobalt citrate, and Ni is referred to as Ni²⁺Examples thereof include nickel nitrate, etc., and Ag is⁺Examples thereof include silver sulfate and silver phosphate.

(2) L-cysteine

L-cysteine is one of sulfur-containing amino acids, is an indispensable component in skin metabolism, contributes to collagen production, and inhibits melanin production by synergistic action with L-ascorbic acid. Is a major constituent of skin, nails, and hair, and is widely distributed in the body. It has been unexpectedly found that L-cysteine not only manifests antibacterial action by itself due to its usage, but also has a molecular structure in which SH group (mercapto group formed by bonding sulfur and hydrogen) is bonded to antibacterial metal ion, amplifies its activity, exhibits strong bactericidal activity, and promotes destruction of bacterial cells by DNA inhibition, enzyme inactivation, inhibition of metabolic function, protein denaturation or generation of free radicals. The strong antioxidant and reducing effects contribute to the stability of the components, have high affinity with the body, strongly adhere to pathogens, and have the function of promoting permeability. The optimum concentration varies slightly depending on the type and concentration of the metal ions contained therein, but is preferably several times the ion concentration. For example, the content of L-cysteine in the disinfectant solution of the present invention is preferably 100 to 1000 ppm.

(3) L-ascorbic acid

The effect of L-ascorbic acid is as described above. The content of the L-ascorbic acid in the sterilizing and disinfecting liquid is preferably 100-500 ppm.

(4) Surfactants other than nonionic surfactants

The basic structure of the surfactant is composed of lipophilic groups and hydrophilic groups, and the functions of the surfactant are wetting, moisture absorption, permeation, solubility assistance, emulsification, dispersion, foaming, lubrication, cleaning, antistatic, adsorption, film formation, antibiosis, cell membrane disturbance, rust prevention and the like in a wide range. The main application of the detergent is completely deep into the daily life of people, such as synthetic detergents, kitchen cleaning agents, toothpaste, hair dye, emulsifying agents, softening agents and the like, and the detergent is known as an indispensable substance at present.

Many surfactants have the above-described action more or less simultaneously, but from the viewpoint of "sterilization and disinfection" and from the viewpoint of use, the following anionic surfactants, cationic surfactants and amphoteric surfactants can be mentioned as the surfactants other than the nonionic surfactants which can exert excellent effects in the present invention.

(anionic surfactant)

Alkyl benzene sulfonates (ABS type), linear alkyl benzene sulfonates (LAS type), polyoxyethylene alkyl ether sulfates (AES type), sodium lauryl sulfate, sodium lauroyl sarcosinate, higher alcohol sulfate salts (AS).

(cationic surfactant)

Octadecyl dimethyl benzyl ammonium chloride, benzalkonium chloride, benzethonium chloride.

(amphoteric surfactant)

Alkyl diamino ethyl glycine hydrochloride, alkyl poly amino ethyl glycine hydrochloride.

The above-mentioned surfactants other than the nonionic surfactant may be used singly or in combination of two or more.

In the disinfectant solution of the present invention, by containing the surfactant other than the nonionic surfactant in an amount of 20 to 100ppm, the disinfecting effect is amplified and the disinfecting time is significantly shortened. The following represents 1 example thereof.

[ Table 5]

TABLE 5 germicidal Effect from the addition of surfactants

(5) Others

The sterilization disinfectant can improve the bactericidal power by containing more than one of sorbic acid, sorbate, benzoic acid, benzoate and p-hydroxybenzoate.

Examples of the sorbate include potassium sorbate and sodium sorbate. Examples of the benzoate salt include potassium benzoate, sodium benzoate, calcium benzoate, ammonium benzoate, and zinc benzoate.

The concentration of the sorbic acid, the potassium sorbate, the benzoic acid, the sodium benzoate and the paraben in the sterilizing disinfectant is preferably 50-100 ppm.

The disinfectant solution of the present invention can be prepared by adding and mixing the above-mentioned various components in water. The order of addition is not particularly limited. The water used as the medium includes tap water, ion-exchanged water, pure water, purified water, and the like, and can be appropriately selected according to the purpose of use.

(6)pH

In addition, the antimicrobial and disinfectant solution of the present invention is adjusted to be acidic, which contributes to the maintenance of the titer and stability of the components of the antimicrobial and disinfectant solution and the penetration into pathogens. The pH value of the sterilization disinfectant is preferably 2.5-4.0. Among them, a known pH adjuster can be used for adjusting pH.

(7) Permeability of

When solutions of different concentrations are separated by a semipermeable membrane, the permeation of a solution of a dilute concentration into a solution of a higher concentration is called osmosis, and the strength thereof is called osmotic pressure. All the main components constituting the sterilizing liquid of the present invention have high affinity for cell tissues. Therefore, by minimizing the amount of the constituent components, a force is exerted to permeate (with a low concentration) into (inside of) a high concentration, thereby realizing a sterilizing/disinfecting solution having a quick action.

Without being limited to germicidal disinfectants, the effectiveness of high concentrations is overwhelmingly well established as a general rule of knowledge. On the other hand, the sterilizing and disinfecting solution of the present invention, which has a phenomenon that a sufficient sterilizing effect is produced even if the amount of the main component is small, has a significant advantage in terms of economy and safety in that it is more effective at a low concentration or at a lower concentration.

Table 6 (1) and (2) show some of the effects of the disinfecting and sterilizing liquid of the present invention prepared by adding L-cysteine to a mixture of various metal ions, L-ascorbic acid and a surfactant to adjust the pH to an acidic pH of 3.0 with dilute hydrochloric acid.

[ Table 6-1]

TABLE 6 (1) Sterilization effects

[ tables 6-2]

TABLE 6 (2) germicidal Effect

As shown in Table 6 (1) and (2), it was confirmed that the test bacteria were killed by contacting them for 10 to 15 seconds at the optimum concentration of the metal ions in an acidic aqueous solution (pH3.0) containing 500ppm of L-cysteine, 100ppm of L-ascorbic acid and a surfactant (sodium lauryl sulfate 100ppm) in a range from the concentration at which the metal ions alone exhibited a strong growth inhibitory effect to the concentration at which the metal ions exhibited an inhibitory effect.

It goes without saying that the optimum concentration of the metal ion varies to a greater or lesser extent depending on the amount of other substances such as L-cysteine added.

Next, a suspension of various test bacteria (about 1X 10) was further prepared⁹Cell/raw drinking water), 2 wt% of the bacterial liquid was dropped into the sterilizing disinfectant of the present invention comprising table 6 No13, one platinum-made selective loop was inoculated into each growth medium over time, and the bacterial growth was observed for the presence or absence of growth of bacteria under optimum conditions. The results are shown in tables (1) and (2) 7.

[ Table 7-1]

TABLE 7 (1) Sterilization effects

+: growth and development, -: does not grow and develop

[ tables 7-2]

TABLE 7 (2) Sterilization effects

+: growth and development, -: does not grow and develop

As can be seen from the above table, if the bacteria are common bacteria, the sterilization disinfectant can kill in 10-15 seconds, and the acid-fast bacteria can kill in 1 minute. In addition, in the case of fungi, the filamentous fungi can be killed in about 15 seconds, and the yeast fungi can be killed in about 30 seconds.

Spores were disintegrated in 1 to 120 minutes depending on the formation stage (see example 12 described later in detail).

In the case of the virus, the enveloped avian influenza virus H5N3 was almost inactivated by contact for 5 minutes, and the non-enveloped norovirus was almost inactivated by contact for 30 minutes (see example 13 described later in detail).

In addition, the antibacterial power of the disinfectant can be further improved by adding a small amount of essential oils and antibacterial components derived from various plants such as cypress and mint or antibacterial components derived from minerals to the disinfectant.

The action mechanism of the disinfectant of the present invention is complicated and not sufficiently decomposed, but it is presumed that the metal ion antibacterial action (the mechanism is centered on a strong oxidizing ability, active oxygen is generated by the action of an ion catalyst, hydrogen peroxide is generated by the reduction of ions, and the cell itself is destroyed by the generation of OH radicals, which are responsible for the strong oxidizing ability, and the protein is coagulated and denatured to damage enzymes and inhibit the metabolic function, and the cell nuclear membrane of bacteria is destroyed by the binding with-SH groups, -COOH groups, -OH groups, etc. of bacteria) is exhibited to the maximum extent, and the killing ability in a very short time is exhibited.

In the present invention, the term "disinfection" means that pathogenic bacteria are killed and that the remaining of nonpathogenic microorganisms does not become a problem. On the other hand, sterilization means that not only pathogenic microorganisms but all microorganisms are killed. The term "disinfectant" as used herein means a disinfectant having either of the above-mentioned disinfecting action and disinfecting action. The term "antimicrobial" means the state from the inhibition of the growth of microorganisms to the killing of microorganisms.

The pathogenic bacteria in the present invention mean microorganisms causing diseases such as bacteria and viruses, and examples thereof include Salmonella spp, Shigella spp, Vibrio parahaemolyticus, Vibrio cholerae, Escherichia coli O-157, Campylobacter jejuni, Clostridium difficile, Clostridium perfringens, Yersinia enterocolitica, Helicobacter pylori, Entezomorpha histolytica, Bacillus coli, Clostridium histolyticum, Clostridium botulinum, Streptococcus pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae, and Streptococcus pneumoniae, Chlamydia pneumoniae (chlamydiae), Legionella pneumophila (Leginella pneumoniae), Branhamella catarrhalis (Branhamella catarrhalis), Mycobacterium tuberculosis (Mycobacterium tuberculosis), Mycoplasma pneumoniae (Mycoplasma pneumae), Streptococcus pyogenes (Storepcoccaceae), Corynebacterium diphtheriae (Corynebacterium diphtheria), Bordetella pertussis (Bordetella pertussis tussisi), Chlamydia psittaci (Chramidium psidium psittaci), Pseudomonas aeruginosa (Pseudomonas aeruginosa), Methylococcus pyogenes (Methylococcus pyogenes), Staphylococcus aureus (Staphylococcus aureus), Escherichia coli (Escherichia coli), Klebsiella pneumoniae (Klebsiella pneumoniae), Escherichia coli (Streptococcus mutans), Streptococcus mutans (Escherichia coli), Streptococcus mutans (Streptococcus mutans), Streptococcus faecalis (Streptococcus mutans, etc.

Examples of the virus in the present invention include avian influenza virus, norovirus, hepatitis virus, human immunodeficiency virus, rotavirus and the like.

The object to be treated can be sterilized by bringing the sterilizing liquid of the present invention into contact with the object.

Examples of the object to be treated include environments, instruments, human bodies, animal bodies, plant bodies, and organic substances.

Examples of the environment include a home environment such as a bedroom, a living room, a toilet, a bathroom, and the like, an environment in a vehicle such as an automobile, a train, an airplane, a ship, and the like, a special environment in which a clean environment is required to be maintained such as an operating room, a ward, a rest room, and the like, an environment in which domestic animals such as cats, rabbits, dogs, chickens, sheep, goats, pigs, cattle, and horses are raised, and a marine farm.

The environment also includes furniture, props, appliances, toys, devices, and the like that are present in the environment.

Examples of the above-mentioned appliances include metal appliances, nonmetal appliances, and appliances combining these appliances.

The human body and the animal body include skin, fingers, and mucous membranes which come into contact with the outside in a daily life, and wound sites, disease-lesion sites, and the like are exemplified.

Examples of the plant body include vegetables, fruits, ornamental plants, and the like.

Examples of the organic substances include blood, body fluid, expectoration, pus, and excrement of human and animals.

The contact method of the sterilizing liquid may be a known method such as spraying or coating. In addition, when the air is sprayed, the air can be sterilized.

Specific production examples and examples will be described below, but the gist of the present invention is not limited to these production examples and examples.

Examples

The disinfecting and sterilizing liquid of the present invention can be produced in various forms by combining the components of the present invention and their concentrations, and only representative examples thereof will be used in the examples described below.

The basic production method is preferably a method in which a solution a containing metal ions and a solution B containing components other than metal ions are separately produced and then mixed (solution a + solution B being the set concentration of each component). The volumes of the solution A and the solution B may be equal to each other, but are preferably about 1: 9 or 2: 8, and accidents such as precipitation of components can be prevented. In addition, in the supplementary note, the precipitation can be clarified with little influence on the sterilization ability by filtering. The adjustment of the pH is preferably performed after mixing using dilute hydrochloric acid, dilute sulfuric acid, or dilute nitric acid.

< production example 1>

Solution A: 0.96g of ferric chloride hexahydrate was dissolved in 200ml of purified water.

Solution B: 1g L-cysteine, 0.1g L-ascorbic acid, 0.05g of potassium sorbate and 0.5g of sodium lauryl sulfate were dissolved in 800ml of purified water.

Then, the solution A and the solution B were mixed, and 1ml of 3N hydrochloric acid was added to prepare a disinfectant solution composed of the following components.

Comprises the following components: fe³⁺Ion 200ppm, L-cysteine 1000ppm, L-ascorbic acid 100ppm, potassium sorbate 50ppm, sodium dodecyl sulfate 100ppm (pH3.0)

< production examples 2 to 10>

Hereinafter, various types of the disinfectant solutions of the present invention were prepared by the same production method, and the solutions are summarized in the list (tables 8 (1) and (2)) as production examples 2 to 10.

TABLE 8-1 preparation examples of the disinfectant

(Metal Compound g/1000ml ═ Metal ion ppm)

TABLE 8-2 preparation examples of the disinfectant

(Metal Compound g/1000ml ═ Metal ion ppm)

< example 1>

The effectiveness of general sterilizing and disinfecting solutions is often reduced in the presence of organic substances. This phenomenon is remarkable in the case of sodium hypochlorite, quaternary ammonium salts and biguanides. The liquid prepared in production example 1 was tested for its effect on the disinfectant of the present invention.

The liquid of 0.1% chlorella extract (CGF) and 1% commercial concocted sauce were prepared, and the sterilizing liquid was added in an amount of 1% and 5%. Next, as test bacteria, suspensions (1X 10) of 1% by weight of S.aureus (MRSA) and E.coli O-157 were inoculated, respectively⁹Cell/raw drinking water). Over timeThe resulting culture was inoculated in a culture medium at 100. mu.L and cultured at 37 ℃ to observe the bactericidal effect of the cells in the presence or absence of bacterial growth, as shown in Table 9.

[ Table 9]

Bactericidal effect in the presence of organic matter of table 9

+: development, -: does not develop

In the presence of organic matter, when the concentration is dilute (below 50 ppm), the sterilization effect is not affected at all. However, if the concentration is 100ppm or more, the effectiveness is slightly lowered, and even if this is done, bacteria are killed within 30 seconds in the presence of 500ppm of organic matter, and inactivation such as hypochlorite is not observed.

< example 2>

The situation where bacteria vigorously proliferate in natural environments occurs not only in the case of food poisoning but also in foods rich in nutrition. In this case, it was also observed whether the disinfectant of the present invention can function effectively. This is the final case of the test in the presence of the organic substance of example 1.

The cooked assorted dishes (with carrot, fried bean curd, burdock, chicken, hizikia fusiforme added), cold jellied bean curd, juice roll, cuttlefish body and boiled pumpkin were each left at 23 ℃ for 24 hours, and then the present disinfectant prepared in production example 9 was sprayed on the surface of each cooked product in multiple passes, and from immediately after spraying to 24 hours, a part of each sample was taken out and homogenized with a homogenizer, and the number of viable bacteria in 1g of food was measured according to a conventional method, and is shown in table 10.

In addition, as a comparative control, water and chlorhexidine solution (a 0.1% solution of chlorhexidine gluconate) were sprayed.

[ Table 10]

10 th table sterilization effect in cooked food

Spray after 24 hours (number of bacteria/g)

The process of food spoilage is naturally different depending on the food material, cooking method, and environment.

For the assorted dishes in this test, 2X 10 after cooking for 24 hours⁶The viable count per gram was increased to 2.5X 10 after 48 hours⁸Per gram.

Similarly, for cold jellied bean curd, after 24 hours, it was 1X 10⁸One/g, increased to 1.5X 10 after 48 hours⁹Per g, slightly rancid.

In the case of juice roll, the volume was 5X 10 after cooking for 24 hours⁶One/g, increased to 1X 10 after 48 hours⁸The number per gram, but no particular abnormality was confirmed in the functional test.

In the case of cuttlefish, the color of the product was 3X 10 after 24 hours⁸Per g, considerable rancidity had occurred, and after 48 hours the bacteria number increased only 10-fold (5X 10)⁹One/g) but emits a strong rancid odor.

In the case of cooked pumpkin, it was 5X 10 after cooking for 24 hours⁶The number of viable bacteria per gram is increased to 8 multiplied by 10 after 48 hours⁷In the functionality test, the number/g was almost unchanged from that immediately after cooking. (all above are sprayed with water only)

When a disinfectant is sprayed in a state where the above-mentioned putrefaction is slightly advanced, viable bacteria are rapidly reduced to 1/1000 to 1/1 ten thousand, 1/1 to 1/100 ten thousand after 1 hour, 1/10 to 1/1000 thousand after 5 hours, and 1/100 to 1/1 billion after 24 hours immediately after spraying the disinfectant, and complete sterilization is not achieved except for a juice roll.

On the other hand, in the case of chlorhexidine solution, the number of viable bacteria immediately after spraying decreased to 1/10 to 1/1000, and continued to decrease after 1 hour, stopped after 5 hours, started to increase thereafter, and increased to a considerable number of bacteria after 24 hours, and no substantial bactericidal effect was observed.

< example 3>

The general disinfectant is not effective for excrement (specimen) or, if effective, is not suitable for use because of its poor effectiveness in many cases. Therefore, tests were conducted on the effectiveness of the disinfectant. In addition, as comparative controls, 70% ethanol, sodium hypochlorite solution (effective chlorine amount 400ppm) and chlorhexidine solution were used.

(1) Expectoration of phlegm

Sputum was collected from 5 persons, mixed and then pretreated with a sputum-dissolving agent Sputazyme, and a part of the sputum was collected to determine the viable cell count according to a usual method. 2 times of each of the sterilizing disinfectants was added to the remaining specimens, and the number of viable bacteria in the specimens was measured over time. The disinfectant prepared in production example 5 was used as the disinfectant (the same disinfectant was used for the following test specimens, feces and pus).

(2) Excrement and urine

Intestinal bacterial survival measurable in feces collected 1.5X 10¹¹Per gram. Adding 5 times of various sterilizing and disinfecting liquids into the excrement, fully stirring, and measuring the number of viable bacteria in the excrement by an anaerobic culture method in time.

(3) Pus discharge

Pus mainly caused by antibiotic-resistant MRSA and pus mainly caused by pseudomonas aeruginosa (MDRP) were collected from patients with bedsores, and the number of viable bacteria per 1g of pus was measured. Subsequently, 2 times the amount of the sterilizing disinfectant was added, and the number of viable bacteria in the specimen was measured over time according to a usual method.

The bactericidal effects on the specimens of (1), (2) and (3) above are shown in table 11.

[ Table 11]

Sterilization effect of 11 th sample (feces) from the surface

The number of viable bacteria in expectoration is 2 × 10⁹However, the amount of the bacteria per gram was one in a thousand after 5 minutes, 1/200 ten thousand after 15 minutes, and all the bacteria were killed after 30 minutes after 2 times of the sterilizing liquid of the present invention was added.

On the other hand, in the case of 70% ethanol, the amount remained reduced to 1/1000 even after 60 minutes, and the survival time was 5X 10⁶Per gram. In the case of sodium hypochlorite, the amount rapidly decreased after the addition, but the amount was almost in this state after the addition, and even after 60 minutes had elapsed, 5X 10 was present³Per gram. In the case of chlorhexidine, a state very similar to hypochlorite was also shown, and survival was confirmed to be 8 × 10 even after 60 minutes⁴Per gram.

Next, the number of viable bacteria that can be measured in feces was 1.5X 10¹¹However, when 5 times of the amount of the present disinfectant was added, the amount of the disinfectant was reduced to about 1/100 after 5 minutes and 1/200 ten thousand after 15 minutes, and the disinfectant did not completely die after 60 minutes, and the survival was confirmed to be about 150 per gram, but the disinfectant was not almost completely sterilized.

In contrast, the bacteria were only slightly reduced in the case of 70% ethanol and chlorhexidine, which proved to be ineffective in practice. In the case of hypochlorite, the amount decreases to about 1/1 ten thousand after 60 minutes. Although not invalid (1X 10)⁷One/g), but it is difficult to say that the composition is an effective performance.

In the case of pus, a significant difference between the MRSA subject itself and the MDRP subject itself could not be confirmed, and all of the bactericidal disinfectant solutions tested were effective.

That is, in the case of the disinfectant solution of the present invention, the disinfectant solution was completely killed after 30 minutes, and in the case of a general disinfectant solution, the disinfectant solution was not completely killed even after 60 minutes of contact, but rapidly decreased to 1X 10²～5×10⁴Per gram.

< example 4>

Then, when used in a cough, the effectiveness was tested. The number of viable bacteria in 1ml of saliva before 3 persons had a cough was measured, and then the disinfectant of the present invention prepared in preparation example 6 was applied to the cough 3 times and 1 time for 20 seconds, i.e., for 1 minute, and the saliva immediately after the cough, 15 minutes, 30 minutes, and 60 minutes was collected, and the number of viable bacteria contained in 1ml of saliva was measured, and the effect of cough was observed. The number of viable bacteria before cough was represented by a numerical value of 100.

As a control, 2 persons had a cough using tap water and 1000ml of povidone-iodine, respectively, in the same manner. These results are shown in Table 12.

[ Table 12]

The 12 th apparent cough relieving effect

The number of bacteria immediately after gargling with tap water alone was 5 to 10%. However, it is unexpectedly found that the state is rapidly returned to the original state as time elapses.

On the other hand, povidone-iodine recommended as a gargle reduced to 2% immediately after gargling, gradually increased thereafter, and returned to a level of 30 to 40% after 60 minutes, which resulted in no significant difference from the gargling with tap water.

On the other hand, when the disinfectant of the present invention is used, the rinse-off effect is 1 to 2% immediately after rinsing, 2 to 5% after 15 minutes, and 8 to 12% after 30 minutes, and is maintained at 15 to 20% even after 60 minutes, thereby clearly showing that the rinse-off effect is sustained for a long period of time. In addition, reports on the reduction or cure of chronic gingivitis and periodontal disease by continuous gargling with the disinfectant for a long time are published.

< example 5>

The skin of the hand is widely coated with the sterilizing disinfectant, and the sterilizing effect is observed.

Immediately after the disinfectant solution prepared in production example 3 was applied, 10cm of sterilized gauze immersed in sterilized raw drinking water was wiped²The number of attached bacteria was measured (smear method). Then, after 15 minutes, 30 minutes and 60 minutes, respectively, the coating was applied to 10cm²Move within the range, and observe each time at 10cm²The bacteria attached to the skin are wiped off. In addition, as a comparative control, tap water and 70% ethanol were used. The number of bacteria before applying the disinfectant solution was expressed as a numerical value of 100. These results are shown in Table 13.

[ Table 13]

Degree of reduction of bacteria after applying disinfectant to No. 13 surface

As can be seen from the above table, tap water certainly did not exhibit a sterilizing effect, and was within the error range. Immediately after coating with 70% ethanol, no bacteria were detected, and thereafter, bacteria were gradually developed (dropped bacteria and adhesion due to movement of bacteria, etc.), and 60 minutes later, the temperature was returned to 45% of that immediately before coating.

On the other hand, bacteria could not be detected immediately after the application of the disinfectant of the present invention, and the detection rate was only 0.01% even after 30 minutes and was only 0.04% even after 60 minutes, and it was confirmed that the effectiveness was maintained.

< example 6>

In the hospital setting, the effectiveness of door handles (stainless steel) and sanitary ware for urination, which are touched by an uncertain public, contaminated with bacteria and fungi, was tested. The samples were used as they were immediately after the wiping, and the growth and contamination state of the attached bacteria immediately after the wiping were observed over time, and the results are shown in table 14.

The disinfectant prepared in production example 7 was used as the disinfectant of the present invention, and tap water was used as a control.

[ Table 14]

14 th Table shows the Sterilization Effect of appliances

The number of bacteria before wiping is 100

The bacteria adhering to the handle before the wiping was completely killed by the disinfectant of the present invention immediately after the wiping, and the effect was maintained for several hours, 1/1 ten thousand even after 6 hours, 1/500 even after 24 hours, and only 1/100 bacteria were recovered even after 48 hours, and it was confirmed in this test that the effectiveness was maintained for a long time.

On the other hand, when wiped with tap water, the bacteria were removed to 1/1000 only immediately after wiping, but the number of bacteria adhered thereafter increased, and returned to about 1/2 after 24 hours and to the state before wiping after 48 hours.

In the urinal test, although the effectiveness of the urinal was not shown to the extent of that of the handle due to the nutrition supplementation (urine), the number of bacteria remained 1/5 even after 24 hours and before wiping was 1/10 even after 48 hours. When tap water was used, the same tendency as that of the door handle was exhibited, and the state was returned to the original state after 48 hours.

< example 7>

The disinfectant prepared in production example 8 was sprayed on a floor (P-tile) with significant contamination, and the moistened sterile gauze was moved by 100 cm/hour²The number of bacteria was measured by wiping, and the growth and disappearance thereof are shown in Table 15. In addition, tap water was used as a control.

[ Table 15]

Effectiveness of 15 th surface sterilizing and disinfecting liquid on ground

(the number of bacteria immediately after spraying was 100)

In the case of tap water, certain bacteria still survive. On the other hand, when the present disinfectant was sprayed, a very small amount of viable bacteria could be observed immediately after spraying, but the viable bacteria were not detected after 15 minutes or 30 minutes, and a very small amount of bacteria was observed after 60 minutes.

This result means that the bacteria are immediately killed even if they adhere to the floor surface in a state where the floor surface is wetted with the disinfectant.

< example 8>

The mat area was taken up in about 8 pieces (about 13 m) with a syringe having a capacity of 100ml²) The air in the closed room of (1) classifies microorganisms contained in the air into general bacteria, spores and fungi, and the number of viable bacteria is measured.

Next, the sterilizing disinfectant of the present invention prepared in production example 9 was injected into a tank of a commercially available Micro Fogger (ultrasonic spray device UV-200SP manufactured by Aquamid Co., Ltd.), sprayed at 20 ml/min (particles of 25 μm), and air was collected at a fixed point with a 100ml syringe over time to examine the number of viable microorganisms contained therein, and the ratio thereof is shown in the following Table 16.

[ Table 16]

16 th table of viable count ratio in indoor environment

As is clear from the above table, the number of viable bacteria of the general bacteria was only 5% at 5 minutes after the operation, 1% at 30 minutes, and all the bacteria were dead at 60 minutes.

The survival rate of the fungi became 35% 5 minutes after the operation, decreased to 8% 30 minutes later, and all died 60 minutes later. In contrast, spores were almost completely unaffected at 5 minutes, but thereafter slowly disintegrated less and after 120 minutes, they were substantially disintegrated (survival rate 1%).

This fact means that if the particles of the present sterilizing and disinfecting liquid are continuously sprayed in a room which is not an open system, the room can be brought into a substantially sterile state, indicating the possibility of developing a novel high-performance sterilizing air cleaner (free from bacteria and viruses).

< example 9>

(1) It is not surprising that the vegetables account for about 30% as an edible material for food poisoning. Vegetables have a problem of residual agricultural chemicals, and it is important to prevent food poisoning by sufficiently washing vegetables in running water when they are eaten raw. In this case, the present disinfectant solution prepared in production example 2 was immersed in vegetables (cabbage, radish sprouts, lettuce) for 2 minutes, followed by rinsing with tap water for 15 seconds, and then the number of viable cells attached to each unit area of the vegetables was measured over time. As a control, the vegetables were washed in running water for 15 seconds. The results are shown in Table 17.

[ Table 17]

Sterilizing and cleaning effect of 17 th surface vegetable

It was confirmed that the test vegetables were completely sterilized immediately after immersion in the present disinfectant for 2 minutes, and thereafter, there were few bacteria adhering thereto, but only simple washing in running water merely performed sterilization.

(2) Fish is known as a food material which is a representative cause of food poisoning because it is putrefactive relatively quickly because a large amount of pathogenic bacteria and putrefactive bacteria grow not only on the surface but also inside the scales. Particularly, people like raw food, so the events are not always after. This study was conducted on herring and sardine whose putrefaction proceeded extremely rapidly. The present disinfectant solution prepared in production example 5 was put into a vessel, and fish bodies were immersed in the solution for 1 minute, and thereafter, the surfaces of the fish bodies were gently rubbed in running water for 20 seconds and left at room temperature (23 ℃).

In addition, fish bodies washed in running water for 20 seconds were used as controls.

The detected bacteria were classified into vibrio and other bacteria which are deep as compared with the source of fish, and the number of viable bacteria before washing and the number of viable bacteria after washing were measured over time, and the results are summarized in table 18.

[ Table 18]

Sterilizing and cleaning effect of 18 th surface fish

The number of bacteria adhered immediately after washing with running water was reduced to 1/200-1/1000, regardless of the type of fish, but the bacteria proliferated gradually when left to stand, and the number of bacteria was larger after 6 hours than before washing. In particular, Vibrio is about 10 times as significant. The reason is unknown, and is presumed to be related to the removal state of mucus on the surface of a fish body.

On the other hand, after the sterilization disinfectant is immersed for 1 minute, bacteria and vibrio cannot be detected immediately after the disinfection disinfectant is extracted, and the bacteria are maintained at a level of 1/20-1/500 of the number of bacteria before washing even after 6 hours (the detected bacteria are mainly generated by the fallen bacteria).

That is, like vegetables, the bactericidal effect is expected to be long-lasting, and it is considered that it can contribute to prevention of food poisoning by fish. In addition, the flavor of the vegetables and the fish is not changed completely by adopting the disinfectant, and the original taste is still kept.

< example 10>

In a pet kept at home, the cleaning effect was observed for 2 guinea pigs and indoor guinea dogs, respectively. The whole body was soaked in tap water heated to 35 ℃ and the present disinfectant prepared in production example 4 for 3 minutes, during which time the body was washed with a light brush. After washing, the body hair was dried with a blower. Immediately thereafter, the number of body surface bacteria was measured and 24 hours later, and hair and body odor were also examined.

As a result, in the case of guinea pigs, 1X 10 cells adhered after washing⁶Per cm²The bacteria can be sterilized to 5 × 10 by washing with tap water⁴Per cm²That is, the bacteria were removed to 1/20, but after 24 hours, both the number of bacteria and the body odor were restored to the level before washing.

On the other hand, when the sterilizing and disinfecting liquid is used, the reduction is 2 multiplied by 10²Per cm²That is, the number of bacteria was reduced to 1/5000, and thereafter, the number of bacteria was gradually restored, and after 24 hours, the level of 1/250 (4X 10) before washing was maintained³Per cm²). Body hair is also glossy and fluffy, and body odor is also slightly odorous.

In the case of indoor dogs, the number of bacteria adhering to the body surface was about 1/10 for guinea pigs, but when washed with the disinfectant, the disinfectant showed a tendency similar to the performance of the guinea pigs, and the body odor hardly disappeared even after 24 hours had passed, and the effect lasted for 72 hours (3 days).

< example 11>

(1) Therapeutic test example 1

A treatment trial was conducted for 3 months on 8 patients with interdigital, vesicular and keratotic trichophytosis (tinea pedis) and 2 patients with toenail trichophytosis.

The treatment test method employed was a flow washing method in which the affected part was soaked in the present disinfecting and sterilizing solution prepared in production example 10, which was charged into a resin container, for 15 minutes, and then the affected part was washed with running water.

It is difficult to cure tinea pedis radically, especially toenail tinea pedis, and even in experts, it is considered to be very difficult to treat. The following table 19 shows the trichophytosis symptoms of the treatment subjects, the microscopic examination results of the skin and toenails collected from the affected part, and the culture examination results of the part pretreated with potassium hydroxide.

[ Table 19]

19 th treatment test for tinea pedis

As can be seen from the above table, the treatment results vary from individual to individual, and vary from almost completely cured to almost non-improved.

However, the trichophyton cultured after 3 months almost became negative except for the cases of toenail and tinea pedis. Indicating the possibility of radical treatment by further continued treatment.

(2) Therapeutic test example 2

5 volunteers suffering from chronic sinusitis were subjected to nasal irrigation 1 time per day for 3 months using the disinfectant prepared in production example 1. The diagnosis was made based on subjective symptoms and pathological findings, and it was confirmed that all 5 persons had reduced symptoms after 1 month, and after 3 months, the symptoms were reduced in both pathological examination and X-ray examination. Complete cure was reported from 1 person who continued the treatment trial for 6 months.

(3) Therapeutic examination example 3

Helicobacter pylori is known to be a causative bacterium of gastric ulcer and duodenal ulcer, and there is a risk of developing gastric cancer if inflammation continues for a long time, and when helicobacter pylori is sterilized, three doses of antibiotics are recommended for 1 week. At present, the increase of resistant bacteria causes the bacteria removal rate to be about 80 to 85%, and it is expected that the extinguishment thereof becomes gradually difficult, and a therapeutic test is conducted on whether the bactericidal disinfectant of the present invention containing amino acids, vitamins and minerals as essential life substances is effective by one of the present inventors who are positive for helicobacter pylori.

About 50ml of the 16ml liquid prepared in No1 diluted 3 times was taken 1 time a day, and the urea breath test was examined at 1 month intervals for 1 week continuously, and the result was negative, confirming the practicability of sterilization of helicobacter pylori in the future.

< example 12>

Collecting sand from a sand field in a park, collecting soil from a river beach, mixing the sand and the soil, drying the mixture with hot air at 80-90 ℃ for 6 hours, adding 1g of the mixture into water, and immediately filtering the mixture with a filter. A part of the filtered water was applied to a glass slide, and spores were stained by a usual method, and the number of stained spores was counted, and the number of spores contained in 1g of soil was counted. Simultaneously, aerobic- (Bacillus development) and anaerobic- (Clostridium development) were cultured and the ratio of the two was measured.

Next, 1g of the above-mentioned dry soil was added to 10ml of a bactericidal disinfectant to be tested, and part of the soil was cultured aerobically and anaerobically over time, and the colonies grown were measured to examine the disintegration rate of spores. The results are shown in Table 20.

[ Table 20]

Time to disintegration of No. 20 epidermal spore and its proportion

It is known that spores of the genus Bacillus or Clostridium are disintegrated by the disinfecting and sterilizing liquid of the present invention at about 50% in 30 minutes, at least 70% in 60 minutes, and almost all of the spores are disintegrated and destroyed in 120 minutes.

In contrast, 70% ethanol was completely ineffective for spores, and 100% disintegrated and destroyed by contacting hypochlorite (available chlorine amount 400ppm) for 60 minutes or more. However, the shorter the contact time, the more the destruction without disintegration, and the soil inactivates hypochlorite very quickly, often showing no effectiveness.

< example 13>

Avian influenza viruses with an envelope were tested.

Using 5 strains of avian influenza virus, H3N2, H4N6, H5N3, H6N6 and H7N7, each of the virus solutions was cultured in SPF chick embryos, and 0.1ml of each virus solution was mixed with 0.5ml of the disinfectant solution of the present invention prepared in preparation example 10. After leaving at room temperature for 10 minutes, the mixture was diluted in 10 stages with phosphate buffer (pH7.2), and then 0.2ml of each dilution was inoculated into each allantoic cavity of 5 day-10 SPF chick embryos. Incubation was continued at 37 ℃ for 3 days. After standing overnight at 4 ℃, virus titer was determined by studying chicken hemagglutination of allantoic fluid. As a control, a phosphate buffer (pH7.2) was used.

As a result, the titer of any virus was reduced to 1/10 to 1/100 ten thousand, and it was confirmed that the present solution showed a very strong inactivation effect on the virus.

In the test for norovirus without envelope, oysters infected with virus and feces of infected persons were immersed in 10 times the amount of the sterilizing disinfectant for 30 minutes, and then a dedicated institution was asked to investigate whether or not the virus was destroyed, and as a result, the virus reaction was negative.

A part of the examples are described above, and finally toxicity tests are carried out.

The toxicity of the disinfectant of the present invention prepared in preparation example 10 was 1ml > orally administered to LD50 mice and 4ml intraperitoneally administered thereto, and the inhibitory activity (cell proliferation inhibitory level) against animal cells was slightly different depending on the test cells (monkey kidney CV-1 cells and human lymphocytes), but about half of the cells were able to proliferate without being inhibited even when diluted 10 times. When the dilution was 1000 to 10000 times, it was confirmed that the inhibition was not at all.

In contrast, strong inhibitory activity remained in chlorhexidine solution (0.1% chlorhexidine gluconate) diluted 10000 times.

The disinfectant solutions of the present invention prepared in other production examples were also low in toxicity similar to the above, and proved to be unprecedented ones having extremely high safety.

In addition, table 21 summarizes the difference in action between the disinfectant solution of the present invention and the disinfectant solution described in patent document 1.

[ Table 21]

TABLE 21 shows comparison of the effects of the disinfectant of the present invention and the disinfectant described in patent document 1

The evaluation was performed under the same conditions, and the larger the "+" number, the more desirable the properties are shown in the table.

As is clear from the results in table 21, the disinfectant solution of the present invention exhibits excellent effects in various aspects as compared with the disinfectant solution disclosed in patent document 1, which the present inventors have proposed.

Among them, the disinfectant of the present invention has a broad antimicrobial spectrum because of its ability to kill viruses, and is excellent in the persistence of the disinfecting ability, and exhibits a remarkable effect of excellent disinfecting ability in the presence of organic substances. The disinfectant of the present invention is excellent in permeability to substances to be contacted, particularly, microbial cells, etc., has no unpleasant odor, is excellent in storage stability, and is less harmful to a subject, and from these viewpoints, it is easy to handle and is excellent in safety.

Industrial applicability

As described above, the disinfectant solution of the present invention is a very convenient disinfectant solution, which is actually called "universal" as compared with conventional disinfectant solutions, and its application fields are extremely wide as a powerful means for preventing and blocking influenza pandemics (pandemics) of a novel influenza that is considered to be a mere time problem and is mutated from highly pathogenic avian influenza virus H5N1, and as a means for disinfecting and eliminating viruses which are indispensable for most advanced medical treatments for increasing the yield of food produced by biotechnology and driving universal cells, as well as for the production of food produced by biotechnology.

Many of the advantages of the invention encompassed by this specification have been set forth in the foregoing description, but it should be understood that the disclosure is merely illustrative in many respects. Various changes may be made in details, particularly in matters of shape, size, and arrangement of parts, without departing from the scope of the invention.

It goes without saying that the scope of the invention is defined by the words of the scope of the appended claims.

Claims (7)

1. A sterilization disinfectant is characterized in that:

metal ions having an antibacterial action, L-cysteine and L-ascorbic acid as main components, a surfactant other than a nonionic surfactant being contained in the main component,

the metal ion having antibacterial effect is trivalent iron ion (Fe)³⁺) Divalent iron ion (Fe)²⁺) Zinc ion (Zn)²⁺) Copper ion (Cu)²⁺) Cobalt ion (Co)²⁺) Nickel ion (Ni)²⁺) Or silver ion (Ag)⁺)，

The concentration of the metal ion having antibacterial action is 50 to 200ppm in the case of trivalent iron ion, 110 to 400ppm in the case of divalent iron ion, 7.5 to 125ppm in the case of zinc ion, 15 to 60ppm in the case of copper ion, 180 to 300ppm in the case of cobalt ion, 85 to 175ppm in the case of nickel ion, 1 to 3ppm in the case of silver ion,

the concentration of L-cysteine is 100 to 1000ppm, the concentration of L-ascorbic acid is 100 to 500ppm,

the surfactant other than the nonionic surfactant is one or more selected from the group consisting of alkyl benzene sulfonate, linear alkyl benzene sulfonate, polyoxyethylene alkyl ether sulfate, higher alcohol sulfate, sodium lauryl sulfate, sodium lauroyl sarcosinate, octadecyl dimethyl benzyl ammonium chloride, benzalkonium chloride, benzethonium chloride, alkyl diamino ethyl glycine hydrochloride and alkyl poly amino ethyl glycine hydrochloride.

2. The sterilizing and disinfecting solution of claim 1, wherein:

the concentration of the surfactant other than the nonionic surfactant is 20 to 100 ppm.

3. The sterilizing and disinfecting solution of claim 1 or 2, wherein:

further contains one or more selected from sorbic acid, sorbate, benzoic acid, benzoate and paraben.

4. The sterilizing and disinfecting solution of claim 3, wherein:

the concentration of sorbic acid, sorbate, benzoic acid, benzoate and paraben is 50-100 ppm.

5. The disinfectant solution according to any one of claims 1 to 4, wherein:

the pH is adjusted to 2.5 to 4.0.

6. A sterilization method, characterized by:

the sterilizing and disinfecting liquid according to any one of claims 1 to 5 is brought into contact with an object to be treated.

7. The sterilization method according to claim 6, wherein:

the object to be treated is an environment, an appliance, a human body, an animal body, a plant body or an organic matter.