HK1074439B - Quinoline derivatives and their use as 5-ht6 ligands - Google Patents

Description

This invention relates to a novel quinoline compound, 3-phenylsulfonyl-8-piperazin-1-yl-quinoline, having pharmacological activity, to processes for its preparation, to compositions containing it and to its use in the preparation of a medicament for the treatment of CNS and other disorders.

WO 98/27081 discloses a series of aryl sulphonamide compounds that are said to be 5-HT₆ receptor antagonists and which are claimed to be useful in the treatment of various CNS disorders. GB-2341549 , WO 99/47516 and WO 99/65906 all disclose a series of indole derivatives that are claimed to have 5-HT₆ receptor affinity. JP 02262627 (Japan Synthetic Rubber Co) describes a series of substituted quinoline derivatives useful as wavelength converting elements. WO 00/42026 (Novo Nordisk) describes a series of quinoline and quinoxaline compounds for use as GLP-1 agonists.

A structurally novel class of compounds has now been found which also possess affinity for the 5-HT₆ receptor. The present invention therefore provides, in a first aspect, a compound of formula (I) or a pharmaceutically acceptable salt thereof: wherein:

• R1 and R2 represent hydrogen;
• R3, R4 and R5 represent hydrogen;
• m represents 1 ;
• n represents 1;
• p represents 1;
• A represents phenyl;

or solvates thereof.

Preferred compounds according to the invention include examples E1-E2 as shown below, or a pharmaceutically acceptable salt thereof.

The compound of formula (I) can form acid addition salts thereof. It will be appreciated that for use in medicine the salts of the compounds of formula (I) should be pharmaceutically acceptable. Suitable pharmaceutically acceptable salts will be apparent to those skilled in the art and include those described in J. Pharm. Sci., 1977, 66, 1-19, such as acid addition salts formed with inorganic acids e.g. hydrochloric, hydrobromic, sulfuric, nitric or phosphoric acid; and organic acids e.g. succinic, maleic, acetic, fumaric, citric, tartaric, benzoic, p-toluenesulfonic, methanesulfonic or naphthalenesulfonic acid. The present invention includes within its scope all possible stoichiometric and non-stoichiometric forms.

The compound of formula (I) may be prepared in crystalline or non-crystalline form, and, if crystalline, may optionally be solvated, eg. as the hydrate. This invention includes within its scope stoichiometric solvates (eg. hydrates) as well as compounds containing variable amounts of solvent (eg. water).

Certain compounds of formula (I) are capable of existing in stereoisomeric forms (e.g. diastereomers and enantiomers) and the invention extends to each of these stereoisomeric forms and to mixtures thereof including racemates. The different stereoisomeric forms may be separated one from the other by the usual methods, or any given isomer may be obtained by stereospecific or asymmetric synthesis. The invention also extends to any tautomeric forms and mixtures thereof.

A compound according to the invention is 3-phenylsulfonyl-8-piperazin-1-yl-quinoline or a pharmaceutically acceptable salt thereof (eg. as the hydrochloride salt), most preferably as the free base (eg. 3-phenylsulfonyl-8-piperazin-1-yl-quinoline).

It has been found that the free base of 3-phenylsulfonyl-8-piperazin-1-yl-quinoline exists in more than one polymorphic form. The present invention extends to all such forms whether in a pure polymorphic form or when admixed with any other material, such as another polymorphic form. Herein, the polymorphic forms of the free base are referred to as Form I and Form II. Each of the said forms may also be referred to herein as the free base as appropriate.

Suitably, the invention provides the free base, suitably as characterised by data provided by at least one of the following: infrared, Raman, X-ray powder diffraction or nuclear magnetic resonance and melting point data as provided herein, including partial spectral data provided herein.

In a further aspect the invention provides 3-phenylsulfonyl-8-piperazin-1-yl-quinoline Form I.

In a further aspect the invention provides 3-phenylsulfonyl-8-piperazin-1-yl-quinoline Form II.

In a particular aspect, the present invention provides 3-phenylsulfonyl-8-piperazin-1-yl-quinoline (Form I), characterised by

1. (i) an infrared spectrum substantially in accordance with Figure 1; and/or
2. (ii) a Raman spectrum substantially in accordance with Figure 2; and/or
3. (iii) an X-ray powder diffraction (XRPD) pattern substantially in accordance with Figure 3.

In a particular aspect, the present invention provides 3-phenylsulfonyl-8-piperazin-1-yl-quinoline (Form II), characterised by

1. (i) an infrared spectrum substantially in accordance with Figure 4; and/or
2. (ii) a Raman spectrum substantially in accordance with Figure 5; and/or
3. (iii) an X-ray powder diffraction (XRPD) pattern substantially in accordance with Figure 6.

As a consequence of greater stability provided by a higher melting point, 3-phenylsulfonyl-8-piperazin-1-yl-quinoline (Form II) is the preferred form of 3-phenylsulfonyl-8-piperazin-1-yl-quinoline.

The present invention also provides a process for the preparation of a compound of formula (I) or a pharmaceutically acceptable salt thereof, which process comprises:

1. (a) reacting a compound of formula (II) wherein R^1a is as defined for R¹ or an N-protecting group, R², R³, R⁴, R⁵, m, n and p are as defined above and L¹ is a leaving group such as iodo or trifluoromethylsulfonyloxy; with a compound of formula A-SO₂H, (or A-SH followed by a subsequent oxidation step) wherein A is as defined above and thereafter as necessary removing an R^1a N-protecting group;
2. (b) deprotecting a compound of formula (I) which is protected; and thereafter optionally
3. (c) interconversion of another compound of formula (Ia) wherein:
   • R¹ and R² independently represent hydrogen or C_1-6 alkyl or R¹ is linked to R² to form a group (CH₂)₂, (CH₂)₃ or (CH₂)₄;
   • R³, R⁴ and R⁵ independently represent hydrogen, halogen, cyano, -CF₃, -CF₃O, C_1-6 alkyl, C_1-6 alkoxy, C_1-6 alkanoyl or a group -CONR⁶R⁷;
   • R⁶ and R⁷ independently represent hydrogen or C_1-6 alkyl or together may be fused to form a 5- to 7- membered aromatic or non-aromatic heterocyclic ring optionally interrupted by an O or S atom;
   • m represents an integer from 1 to 4, such that when m is an integer greater than, two R² groups may instead be linked to form a group CH₂, (CH₂)₂ or (CH₂)₃;
   • n represents an integer from 1 to 3;
   • p represents 1 or 2;
   • A represents a group -Ar¹ or - Ar²Ar³;
   • Ar¹, Ar² and Ar³ independently represent an aryl group or a heteroaryl group, both of which may be optionally substituted by one or more (eg. 1, 2 or 3) substituents which may be the same or different, and which are selected from the group consisting of halogen, hydroxy, cyano, nitro, trifluoromethyl, trifluoromethoxy, C_1-6 alkyl, trifluoromethanesulfonyloxy, pentafluoroethyl, C_1-6 alkoxy, arylC_1-6 alkoxy, C_1-6 alkylthio, C_1-6 alkoxyC_1-6 alkyl, C_3-7 cycloalkylC_1-6 alkoxy, C_1-6 alkanoyl, C_1-6 alkoxycarbonyl, C_1-6 alkylsulfonyl, C_1-6 alkylsulfinyl, C_1-6 alkylsulfonyloxy, C_1-6 alkylsulfonylC_1-6 alkyl, arylsulfonyl, arylsulfonyloxy, arylsulfonylC_1-6 alkyl, C_1-6 alkylsulfonamido, C_1-6 alkylamido, C_1-6 alkylsufonamidoC_1-6 alkyl, C_1-6 alkylamidoC_1-6 alkyl, arylsulfonamido, arylcarboxamido, arylsuffonamidoC_1-6 alkyl, arylcarboxamidoC_1-6 alkyl, aroyl, aroylC_1-6 alkyl, arylC_1-6 alkanoyl, or a group CONR⁸R⁹ or SO₂NR⁸R⁹, wherein R⁸ and R⁹ independently represent hydrogen or C_1-6 alkyl or together may be fused to form a 5- to 7- membered aromatic or non-aromatic heterocyclic ring optionally interrupted by an O or S atom;
   • and/or forming a pharmaceutically acceptable salt and/or solvate.
   Alkyl groups, whether alone or as part of another group, may be straight chain or branched and the groups alkoxy and alkanoyl shall be interpreted similarly. Alkyl moieties are more preferably C_1-4alkyl, eg. methyl or ethyl. The term 'halogen' is used herein to describe, unless otherwise stated, a group selected from fluorine, chlorine, bromine or iodine. The term "aryl" includes phenyl and naphthyl. The term "heteroaryl" is intended to mean a 5-7 membered monocyclic aromatic o^- a fused 8-10 membered bicyclic aromatic ring containing 1 to 3 heteroatoms selected from oxygen, nitrogen and sulphur. Suitable examples of such monocyclic aromatic rings include thienyl, furyl, pyrrolyl, triazolyl, imidazolyl, oxazolyl, thiazolyl, oxadiazolyl, isothiazolyl, isoxazolyl, thiadiazolyl, pyrazolyl, pyrimidyl, pyridazinyl, pyrazinyl and pyridyl. Suitable examples of such fused aromatic rings include benzofused aromatic rings such as quinolinyl, isoquinolinyl, quinazolinyl, quinoxalinyl, cinnolinyl, naphthyridinyl, indolyl, indazolyl, pyrrolopyridinyl, benzofuranyl, benzothienyl, benzimidazolyl, benzoxazolyl, benzisoxazolyl, benzothiazolyl, benzisothiazolyl, benzoxadiazolyl, benzothiadiazolyl and the like. Heteroaryl groups, as described above, may be linked to the remainder of the molecule via a carbon atom or, when present, a suitable nitrogen atom except where otherwise indicated above. It will be appreciated that wherein the above mentioned aryl or heteroaryl groups have more than one substituent, said substituents may be linked to form a ring, for example a carboxyl and amine group may be linked to form an amide group. The present invention also provides a further process for the preparation of a compound of formula (I) or a pharmaceutically acceptable salt thereof, which process comprises:
4. (d) reacting a compound of formula (IV) with a compound of formula (V) wherein R^1a, R², R³, R⁴, R⁵, A, m, n and p are as defined above, and L² represents a suitable leaving group, such as a halogen atom and thereafter as necessary removing an R^1a N-protecting group; or
5. (e) reacting a compound of formula (VI) with a compound of formula (VII)

wherein R^1a, R², R³, R⁴, R⁵, m, n, p and A are as defined above and L³ represents a suitable leaving group, such as a halogen atom (e.g. a bromine or iodine atom) or a trifluoromethylsulfonyloxy group, and thereafter as necessary removing an R^1a N-protecting group. The N-protecting group used may be any conventional group e.g. t-butyloxycarbonyl (Boc) or benzyloxycarbonyl. Further N-protecting groups which may be used include methyl.

Process (a) wherein a compound of formula (II) is reacted with a compound of formula A-SO₂H typically comprises use of basic conditions and may be most conveniently carried out by using a suitable salt of the compound A-SO₂H (e.g. the sodium salt) in an appropriate solvent such as N,N-dimethylformamide, in the presence of a transition metal salt such as copper (I) iodide.

Process (a) wherein a compound of formula (II) is reacted with a compound of formula A-SH typically comprises use of basic conditions e.g. by using a suitable salt of the compound A-SH (e.g. the sodium salt) in an appropriate solvent such as N,N-dimethylformamide, in the presence of a suitable metal salt such as copper (I) iodide, followed by use of a suitable oxidant such as 3-chloroperbenzoic acid, peracetic acid or potassium monopersulfate.

In processes (a) and (b), examples of protecting groups and the means for their removal can be found in T. W. Greene 'Protective Groups in Organic Synthesis' ( Wiley and Sons, 1991). Suitable amine protecting groups include sulphonyl (e.g. tosyl), acyl (e.g. acetyl, 2',2',2'-trichloroethoxycarbonyl, benzyloxycarbonyl or t-butoxycarbonyl) and arylalkyl (e.g. benzyl), which may be removed by hydrolysis (e.g. using an acid such as hydrochloric acid) or reductively (e.g. hydrogenolysis of a benzyl group or reductive removal of a 2',2',2'-trichloroethoxycarbonyl group using zinc in acetic acid) as appropriate. Other suitable amine protecting groups include trifluoroacetyl (-COCF₃) which may be removed by base catalysed hydrolysis or a solid phase resin bound benzyl group, such as a Merrifield resin bound 2,6-dimethoxybenzyl group (Ellman linker), which may be removed by acid catalysed hydrolysis, for example with trifluoroacetic acid. A further amine protecting group includes methyl which may be removed using standard methods for N-dealkylation (e.g. 1-chloroethyl chloroformate under basic conditions followed by treatment with methanol).

Process (c) may be performed using conventional interconversion procedures such as epimerisation, oxidation, reduction, reductive alkylation, alkylation, nucleophilic or electrophilic aromatic substitution, ester hydrolysis or amide bond formation. For example, N-dealkylation of a compound of formula (la) wherein R¹ represents an alkyl group to give a compound of formula (I) wherein R¹ represents hydrogen. It will be appreciated that such interconversion may be interconversion of protected derivatives of formula (Ia) which may subsequently be deprotected following interconversion.

Process (d) may be performed in the presence of a suitable base, such as sodium carbonate and the use of a suitable solvent such as n-butanol.

Process (e) may be performed in the presence of a palladium, nickel or copper catalyst, for example a mixture of a palladium source such as Pd₂(dba)₃ and a suitable ligand such as (R)-, (S)- or (±)-2,2'-bis(diphenylphosphino)-1,1'-binaphthyl (BINAP) or (2-dicyclohexylphosphanylphenyl)-dimethylamine or 1,1'-bis-diphenylphosphinoferrocene, together with a suitable base such as sodium t-butoxide, in an inert solvent such as 1,4-dioxane.

Compounds of formula (II) may be prepared by reacting a compound of formula (II) wherein R³, R⁴, R⁵, n and L¹ are as defined above, with a compound of formula (IV) as defined above. This process typically comprises the use of a suitable base, such as sodium carbonate and the use of a suitable solvent such as n-butanol.

Compounds of formula (V) may be prepared in accordance with the following scheme: wherein R³, R⁴, R⁵, n, A and L¹ are as defined above.

Step (i) typically comprises reaction of a compound of formula (VIII) with a compound of formula A-SO₂-M, wherein A is as defined above and M is a metal residue such as sodium or potassium, in the presence of a copper (I) salt, e.g. copper (I) triflate or copper (I) iodide, in a suitable solvent such as anhydrous N,N-dimethylformamide or 1,4-dioxane, optionally including a ligand such as N,N-dimethyl-ethylene-1,2-diamine.

Alternatively, the transformation shown in step (i) may be carried out using a two step procedure typically comprising steps (iii) and (iv).

Step (iii) typically comprises the reaction of a compound of formula (VIII) with a compound of formula A-SH, wherein A is as defined above, in the presence of a base such as sodium hydride or potassium phosphate in a suitable solvent such as anhydrous N,N-dimethylformamide or ethylene glycol, optionally in the presence of a copper (I) iodide catalyst.

Step (iv) typically comprises oxidation using a suitable oxidant such as monomagnesium peroxyphthalate, 3-chloroperbenzoic acid, peracetic acid or potassium monopersulfate.

Step (ii) typically comprises the use of a suitable reducing agent, for example titanium (III) chloride, or iron powder in an appropriate solvent system, e.g. aqueous tetrahydrofuran and/or acetic acid, respectively.

Compounds of formula (VI) wherein L³ represents a halogen atom may be prepared in accordance with the following scheme: wherein R³, R⁴, R⁵, n and A are as defined above and Hal represents a halogen atom.

Step (i) typically comprises diazotisation according to known methods (e.g. using sodium nitrite with aqueous inorganic acid as solvent, or an alkyl nitrite ester using a suitable solvent such as acetonitrile in the presence of anhydrous acid e.g. trifluoroacetic acid), followed by treatment of the resulting diazonium salt with an appropriate halide salt such as copper (I) bromide, potassium iodide or tetrabutylammonium iodide. Such a procedure may be carried out in aqueous solution or using anhydrous conditions, for example using trifluoroacetic acid as solvent.

Compounds of formula (VI) wherein L³ represents a halogen atom may also be prepared in accordance with the following scheme: wherein R³, R⁴, R⁵, A and n are as defined above and Hal represents a halogen atom.

Step (i) typically comprises the use of a suitable reducing agent such as iron powder, to give a compound of formula (XI).

Step (ii) typically comprises a diazotisation reaction using an aqueous or non-aqueous source of nitrosonium ions as described above, followed by conversion to a halide.

Step (iii) typically comprises the use of a suitable oxidant such as monomagnesium peroxyphthalate.

Compounds of formula (VI) wherein L³ represents a trifluoromethylsulfonyloxy group may be prepared from compounds of formula (V) as defined above, by diazotisation according to known methods, followed by heating under acidic conditions, followed by treatment with trifluoromethylsulfonic anhydride in the presence of a base, such as pyridine.

Compounds of formula (III), (IV), (VII) and (VIII) are known in the literature or can be prepared by analogous methods.

Pharmaceutically acceptable salts may be prepared conventionally by reaction with the appropriate acid or acid derivative.

The compound of formula (I) and its pharmaceutically acceptable salts have affinity for the 5-HT₆ receptor and are believed to be of potential use in the treatment of certain CNS disorders such as anxiety, depression, epilepsy, obsessive compulsive disorders, migraine, cognitive memory disorders (e.g. Alzheimers disease, age related cognitive decline and mild cognitive impairment), Parkinsons Disease, ADHD (Attention Deficit Disorder/Hyperactivity Syndrome), sleep disorders (including disturbances of Circadian rhythm), feeding disorders such as anorexia and bulimia, panic attacks, withdrawal from drug abuse such as cocaine, ethanol, nicotine and benzodiazepines, schizophrenia (in particular cognitive deficits of schizophrenia), stroke and also disorders associated with spinal trauma and/or head injury such as hydrocephalus. The compound of the invention is also expected to be of use in the treatment of certain GI (gastrointestinal) disorders such as IBS (Irritable Bowel Syndrome). The compound of the invention is also expected to be of use in the treatment of obesity.

Thus the invention also provides a compound of formula (I) or a pharmaceutically acceptable salt thereof, for use as a therapeutic substance, in particular in the treatment or prophylaxis of the above disorders. In particular the invention provides for a compound of formula (I) or a pharmaceutically acceptable salt thereof, for use in the treatment of depression, anxiety, Alzheimers disease, age related cognitive decline, ADHD, obesity, mild cognitive impairment, schizophrenia, cognitive deficits in schizophrenia and stroke.

In another aspect, the invention provides the use of a compound of formula (I) or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for use in the treatment or prophylaxis of the above disorders.

5-HT₆ antagonists have the potential to be capable of increasing basal and learning-induced polysialylated neuron cell frequency in brain regions such as the rat medial temporal lobe and associated hippocampus, as described in International Patent Application No. PCT/EP03/00462 . Thus, according to a further aspect of the present invention, we provide the use of a compound of formula (I) or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for promoting neuronal growth within the central nervous system of a mammal.

In order to use the compound of formula (I) in therapy, it will normally be formulated into a pharmaceutical composition in accordance with standard pharmaceutical practice. The present invention also provides a pharmaceutical composition, which comprises a compound of formula (I) or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.

A pharmaceutical composition of the invention, which may be prepared by admixture, suitably at ambient temperature and atmospheric pressure, is usually adapted for oral, parenteral or rectal administration and, as such, may be in the form of tablets, capsules, oral liquid preparations, powders, granules, lozenges, reconstitutable powders, injectable or infusable solutions or suspensions or suppositories. Orally administrable compositions are generally preferred.

Tablets and capsules for oral administration may be in unit dose form, and may contain conventional excipients, such as binding agents, fillers, tabletting lubricants, disintegrants and acceptable wetting agents. The tablets may be coated according to methods well known in normal pharmaceutical practice.

Oral liquid preparations may be in the form of, for example, aqueous or oily suspension, solutions, emulsions, syrups or elixirs, or may be in the form of a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, emulsifying agents, non-aqueous vehicles (which may include edible oils), preservatives, and, if desired, conventional flavourings or colourants.

For parenteral administration, fluid unit dosage forms are prepared utilising a compound of the invention or pharmaceutically acceptable salt thereof and a sterile vehicle. The compound, depending on the vehicle and concentration used, can be either suspended or dissolved in the vehicle. In preparing solutions, the compound can be dissolved for injection and filter sterilised before filling into a suitable vial or ampoule and sealing. Advantageously, adjuvants such as a local anaesthetic, preservatives and buffering agents are dissolved in the vehicle. To enhance the stability, the composition can be frozen after filling into the vial and the water removed under vacuum. Parenteral suspensions are prepared in substantially the same manner, except that the compound is suspended in the vehicle instead of being dissolved, and sterilization cannot be accomplished by filtration. The compound can be sterilised by exposure to ethylene oxide before suspension in a sterile vehicle. Advantageously, a surfactant or wetting agent is included in the composition to facilitate uniform distribution of the compound.

The composition may contain from 0.1 % to 99% by weight, preferably from 10 to 60% by weight, of the active material, depending on the method of administration

The dose of the compound used in the treatment of the aforementioned disorders will vary in the usual way with the seriousness of the disorders, the weight of the sufferer, and other similar factors. However, as a general guide suitable unit doses may be 0.05 to 1000 mg, more suitably 0.05 to 200 mg, for example 20 to 40 mg; and such unit doses will preferably be administered once a day, although administration more than once a day may be required; and such therapy may extend for a number of weeks or months.

The following Descriptions and Examples illustrate the preparation of compounds of the invention.

Description 1 3-Bromo-8-(4-methy)-piperazin-1-yl)-quinoline (D1)

bis-(2-Chloro-ethylrmethyl-amine hydrochloride (3.7g, 19.2mmol) and sodium carbonate (9.0g, 85mmol) were added to a suspension of 3-bromo-quinolin-8-ylamine (3.9g, 17.5mmol) (for synthesis see Gershon et al., Monatsh. Chem., 199, 122, 935) in n-butanol (70ml). The stirred suspension was heated at reflux for 72h. The reaction mixture was cooled to ambient temperature, diluted with dichloromethane (300ml) and the solution washed with water (300ml), dried (MgSO₄) and concentrated in vacuo to an oil. The oil was purified by chromatography over silica gel eluting with a gradient of methanol/dichloromethane to afford the title compound (D1) as an oil (2.6g, 8.5mmol, 49%); δ_H (CDCl₃) 2.43 (3H, s), 2.78 (4H, br s), 3.44 (4H, br, s), 7.14 (1H, d, J = 6.8Hz), 7.33 (1H, d, J = 7.4Hz), 7.47 (1H, dd, J = 7.8Hz), 8.25 (1H, d, J = 2.3Hz), 8.85 (1H, d, J = 2.3Hz). Mass Spectrum : C₁₄H₁₆BrN₃ requires 305/307; found 306/308 (MH⁺).

Description 2 3-lodo-8-(4-methyl-piperazln-1-yl)-quinoline (D2)

A mixture of 3-bromo-8-(4-methyl-piperazin-1-yl)-quinoline (D1)(1.75g, 5.7mmol), copper (I) iodide (5.4g, 28.5mmol) and potassium iodide (9.6g, 57.8mmol) in hexamethylphosphoramide (20ml) was heated in an oil bath at 150°C for 21 h under argon. To the cooled reaction mixture was added toluene (120ml) and 1 M hydrochloric acid (120ml) and the whole was shaken vigorously for 5 minutes. The insoluble brown solid was then collected by filtration, washed with methanol (3 x 40ml) and resuspended in a mixture of dichloromethane (150ml) and 2M sodium hydroxide (150ml). After shaking the mixture vigorously, the insoluble material was filtered, washed with dichloromethane (2 x 50ml) and discarded. The filtrate and washings were transferred to a separating funnel and the layers were separated. The aqueous phase was extracted with dichloromethane (2 x 100ml) and the combined organic extracts were dried (MgSO₄) and concentrated to a brown oil (1.5g) which was identified by NMR spectroscopy as a mixture of the title compound (D2) and 3-bromo-8-(4-methyl-piperazin-1-yl)-quinoline (D1) in a ratio of 4:1. This mixture was used directly in the next stage (see Example 1). 3-Iodo-8-(4-methyl-piperazin-1-yl)-quinoline (D2): δ_H (CDCl₃) 2.41 (3H, s), 2.76 (4H, br s), 3.42 (4H, br s), 7.14 (1H, d, J = 6.8Hz), 7.29 (1H, d, J = 7.4Hz), 7.44 (1H, dd J = 7.8Hz), 8.47 (1H, d, J = 2.3Hz), 8.98 (1H, d, J = 2.3Hz); Mass Spectrum : C₁₄H₁₆IN₃ requires 353; found 354 (MH⁺).

Description 3 3-Iodo-8-nitroquinoline (D3)

A stirred mixture of 8-nitroquinoline (100 g, 0.57 mol) in acetic acid (500 ml) was treated with N-iodosuccinimide (155 g, 0.69 mol) portionwise over 10 minutes, and warmed to 62°C for 6 h. A further portion of N-iodosuccinimide (25 g, 0.14 mol) was introduced and the mixture stirred for a further 16 h before cooling to ambient temperature. The solvent was removed in vacuo, keeping the temperature below 35 °C. The residue was dissolved in dichloromethane (2 L) and washed successively with saturated aqueous sodium bicarbonate solution (2 x 1L), 10% aqueous sodium thiosulphate solution (1 L), water (1 L), brine (100 ml), then the organic phase was dried over magnesium sulphate. The mixture was filtered and the solvent removed to give a yellow solid which was recrystallised from ethyl acetate to give the title compound (D3) (168 g, 97%) as a yellow solid; δ_H (CDCl₃) 7.65 (1H, app.t), 7.94 (1H, dd), 8.07 (1H, dd), 8.66 (1H, d, J = 2Hz), 9.19 (1H, d, J = 2Hz); Mass Spectrum : C₉H₅IN₂ requires 300; found 301 (MH⁺).

Description 4 8-Nitro-3-phenylsulfonylquinoline (D4)

3-Iodo-8-nitroquinoline (D3) (135 g, 0.45 mol), was suspended in dimethylformamide (2.4 L) in a 5 L 3-necked flask fitted with an overhead stirrer, under an argon atmosphere. This mixture was treated successively with anhydrous sodium phenylsulfinate (99.6 g 0.608 mol), and bis-(copper (I) triflate) benzene complex (70 g, 0.338 mol). The resulting slurry was heated to 65 °C for 18 h. The mixture was cooled, filtered and the solvent evaporated in vacuo. Acetone (2.5 L) was added to the residue and the solution filtered. The filtrate was evaporated in vacuo, a further 2.5 L of acetone added and the mixture filtered again. The solvent was evaporated in vacuo and the residue dissolved in chloroform (3 L) and washed with 10% aqueous ammonia (2 x 2 L), and the organic phase was dried over magnesium sulphate and the solvent evaporated in vacuo. The dark brown residue was purified using a Biotage flash-150 chromatography apparatus (5 kg silica gel) eluting with hexane and increasing proportions of ethyl acetate to give the title compound (D4) (81.5 g, 58%) as a yellow solid; δ_H (d6-DMSO) 7.67 (2H, t), 7.57 (1H, d, 7.96 (1H, t), 8.13 (2H, d), 8.51 (1H, d), 8.59 (1H, d), 9.42 (1H, d), 9.50 (1H, d); Mass Spectrum : C₁₅H₁₀SO₄N₂ requires 314; found 315 (MH⁺).

Description 5 8-Amino-3-phenylsulfonylquinoline (D5)

A slurry of 8-nitro-3-phenylsulfonylquinoline (D4) (46.7 g, 172 mmol), in tetrahydrofuran (750 ml) was added to a stirred solution of 30% titanium (III) chloride in aqueous HCl (470 ml) [Supplied by BDH] cooled in an ice bath, at such a rate that the temperature was maintained below 35°C. Once the addition was completed, the solution was stirred for a further 10 minutes then water (1.5 L) was introduced and the mixture poured into a 5 L beaker. The rapidly stirred solution was treated by portionwise addition of solid potassium carbonate in order to attain pH -8.5. EDTA (250 g, 0.86 mol) was added and followed by further potassium carbonate to maintain pH -8.5. The mixture was extracted with dichloromethane (3 x 1 L) and the combined organic phase passed through a silica plug (500 g) eluting with further dichloromethane (1 L) and 10% ethyl acetate in dichloromethane (1 L). The combined organic phases were evaporated and the residue subjected to purification using Biotage Flash-75 chromatography apparatus (2 kg silica gel), eluting with dichloromethane and increasing proportions of ether to give the title compound (D5) (34.5 g, 72%) as a pale brown solid; δ_H (CDCl₃) 5.0 (2H, br s). 7.02 (1H, dd), 7.25 (1H, dd), 7.44 (1H, t), 7.50-7.59 (3H, m), 8.00-8.40 (2H, m), 8.70 (1 H, s), 0.09 (1H, s); Mass Spectrum : C₁₅H₁₂SO₂N₂ requires 284; found 285 (MH⁺).

Description 6 8-lodo-3-phenylsulfonylquinoline (D6)

8-Amino-3-phenylsulfonylquinoline (D5) (31.6 g, 0.11 mol) was dissolved in trifluoroacetic acid (60 ml) and the mixture evaporated. The resulting brown oil was dissolved in acetonitrile (200 ml) and added dropwise to a stirred solution of n-butyl nitrite (6.1 ml) in acetonitrile (300 ml) maintained at a temperature of <5°C. Once the addition was completed, the mixture was stirred for five minutes then tetra-(n-butyl)ammonium iodide (82 g, 0.22 mol) added portionwise, keeping the temperature below 10°C. The mixture was stirred for a further 20 minutes then concentrated in vacuo. The dark residue was subjected to flash-75 chromatography (2 kg silica gel), eluting with hexane and dichloromethane to give a brown solid. This was dissolved in dichloromethane (500 ml) and washed with 10% aqueous sodium thiosulphate (2 x 300 ml), dried over magnesium sulphate and concentrated to an orange solid. This was triturated with methanol to give the title compound (D6) (25.2 g, 75%) as a light yellow solid; δ_H (CDCl₃) 7.39 (1H, t), 7.53-7.63 (3H, m), 7.96 (1H, d), 8.04 (2H, dd), 8.50 (1H, dd), 8.79 (1H, d), 9.32 (1H, d); Mass Spectrum : C₁₅H₁₀NO₂SI requires 395; found 396 (MH⁺).

Description 7 8-(4-t-Butoxycarbonyl)piperazin-1-yl-3-phenylsulfonylquinoline (D7)

8-Iodo-3-phenylsulfonylquinoline (D6) (25.2 g, 63.6 mmol) was dissolved in dry, degassed dioxan (500 ml) under argon. To this solution was added sodium t-butoxide (8.56 g, 89.2 mmol) and 1-t-butyloxycarbonyl piperazine (14.2 g, 76.4 mmol) followed by a suspension of catalyst under argon. The catalyst was prepared by sonication of a mixture of tris-(dibenzylideneacetone)dipalladium(0) (1.75 g, 1.91 mmol) and 2-dicyclohexylphosphino-2'-(N,N-dimethyl amino)biphenyl (2.25 g, 5.73 mmol) in dry degassed dioxane (10 ml) for 2 minutes. This mixture was stirred at 40 °C for 5 h after which a further charge of catalyst was administered (prepared as above on half the scale) and stirring continued for 16 h at 40 °C.

The mixture was filtered and the solvent removed. The residue was adsorbed onto silica and chromatographed on silica eluting with 1% methanol in dichloromethane to give the title compound (D7) (22.0 g, 76%) as a yellow solid; δ_H (CDCl₃) 1.49 (9H, t), 3.31 (4H, m) 3.72 (4H, m), 7.25 (1H, m), 7.52 (2H, t) 7.57 (3H, m) 8.00 (2H, m) 8.76 (1 H, d) 9.21 (1 H, d); Mass Spectrum: C₂₄H₂₇N₃O₄S requires 453; found 454 (MH⁺).

Description 4 (Alternative Procedure) 8-Nitro-3-phenylsulfonylquinoline (D4)

A 2L vessel was charged with 3-lodo-8-nitroquinoline (D3) (70.8g, 236 mmol), copper (I) iodide (2.25g, 11.8 mmol, 5mol%), potassium phosphate (100g, 472 mmol, 2eq), ethylene glycol (0.71 L, 10 vol) and benzenethiol (36.2 ml, 354 mmol). The mixture was stirred and heated at 80°C for 3.5 hours. The reaction mixture was cooled to 20°C then H₂O (700 ml) and dichloromethane (700 ml) were added, the mixture was stirred for 5 minutes then the lower organic layer was removed (contained solids Charcoal (35.4g, Norit SX) was added to the organic layer and the mixture was stirred at room temperature for 15min then filtered through GF/F filter paper. The filter cake was rinsed with dichloromethane (140 ml) and the combined filtrate was washed with H₂O (350 ml). The resulting dichloromethane solution was added to a suspension of magnesium monoperoxyphthalic acid hexahydrate (210g, 424 mmol, 1.8eq) in a mixture of dichloromethane (700 ml) and methanol (140 ml) over 45 minutes maintaining 18°C < 23°C. The resulting mixture was stirred rapidly for 2.25 hours at 20°C to 23°C, then 10% w/v aqueous sodium sulfite (700 ml) was added over 15 minutes the mixture was separated and treated with saturated aqueous sodium bicarbonate (280 ml). The mixture was stirred for 20 min before the layers were allowed to settle. The lower organic layer was removed, washed with water (280 ml), then concentrated at atmospheric pressure to -210 ml. The resulting mixture was cooled to 0°C, stirred for 2hrs then filtered. The filter cake was washed with cold (0-5°C) dichloromethane (70 ml) then dried in vacuo at 25 to 40°C to g ve the title compound (D4) as a light yellow solid in 64-66% yield, identical spectroscopically to that prepared by the earlier method.

Description 8 8-Amino-3-phenylsulfonylquinoline methanesulfonic acid salt (D8)

A suspension of iron powder (26.7 g, 5 eq, 325 mesh) in THF (300 ml, 10 vol), water (30 ml, 1 vol) and acetic acid (19.2 ml, 3.5 eq) was heated to 50°C. 8-Nitro-3-phenylsulfonylquinoline (D4) (30 g, 1 wt) was added portionwise to the mixture over 30 min, keeping the temperature below 60°C. The reaction mixture was stirred at 50 to 55°C for 60 min. Toluene (240 ml, 8 vol) was added, followed by water (60 ml, 2 vol) before cooling to 40°C and filtering the mixture through a silica gel plug. The silica plug was washed with toluene (2 x 60 ml, 2 vol). The layers of the combined filtrate were separated and the organic layer concentrated in vacuo to ca 10 volumes. The reaction mixture was warmed to 77°C then treated with methanesulfonic acid (7.42 ml, 1.2 eq) added over 15 min maintaining the temperature at 75 to 80°C. The resulting orange suspension was cooled slowly to ambient, stirred at ambient temperature for ca 2h, then the product filtered and washed with toluene (3x 60 ml). The resulting pink solid (D8) was dried in vacuo at ca 45°C to constant weight. Yied: 34.17 g, 94 %. δ_H (d6-DMSO) 2.46 (3H, s), 7.54 (1H, d, J=8Hz), 7.60-7.70 (3H,m), 7.70-7.75 (1H, t, J=8Hz), 7.81 (1H, J=8Hz), 8.13 (2H, d, J=8hz), 8.28 (3H, bs), 9.14 (1H, d, J=2Hz), and 9.28 (1 H, J= 2Hz).

Description 6 (Alternative Procedure) 8-lodo-3-phenylsulfonylquinoline (D6)

A solution of sodium nitrite (5.44 g, 78.8 mmol, 1.2 eq) in water (125 ml, 5 vol) was added to a stirred slurry of 8-amino-3-phenylsulfonylquinoline methanesulfonic acid salt (D8) (25.0 g, 65.7 mmol) in 5M HCl (500 ml, 20 vol). The mixture was stirred at 23° to 24.5°C for 1hr 5 min then acetonitrile (200 ml, 8vol) was added. After 10 min a solution of sodium iodide (14.8g, 98.6 mmol, 1.5eq) in water (125 ml, 5 vol) was added over 3 min, resulting in the formation of a brown mixture and the evolution of gas. The brown mixture was stirred at 25°C to 23°C for 1 hr 5min then dichloromethane (500 ml, 20 vol) was added and the mixture was stirred for 5 min The lower organic layer was removed and the aqueous layer was extracted with dichloromethane (125 ml, 5 vol). The combined organic layers were washed with 10%w/v sodium sulfite (125 ml, 5 vol) then concentrated under reduced pressure. The resulting mixture was filtered and the cake was washed with acetonitrile (2 x 25 ml) and dried in a 40°C oven under reduced pressure to afford the title compound D6; yield 15.27g, 59%, identical spectroscopically to that produced by the first method.

Reference Example 1 8-(4-Methyl-piperazin-1-yl)-3-phenylsulfonylquinoline (Ref E1)

A 4:1 mixture of 3-iodo-8-(4-methyl-piperazin-1-yl)-quinoline (D2) and 3-bromo-8-(4-methyl-piperazin-1-yl)-quinoline (D1) (1.5g), phenylsulfinic acid sodium salt, dihyd-ate (2.52g, 12.6mmol) and copper (I) iodide (2.4g, 12.6mmol) in N,N-dimethylformamide (25ml) was stirred in an oil bath at 120°C for 40h under argon. To the reaction mixture, cooled to ambient temperature, was added 5% sodium hydrogen carbonate solution (100ml) and dichloromethane (100ml) with vigorous shaking. The insoluble material was filtered, washed with dichloromethane (3 x 20ml) and discarded. The filtrate and washings were transferred to a separating funnel and the layers separated. The aqueous layer was extracted with dichloromethane (1 00ml) and tie combined organic extracts were washed with water (100ml), dried (MgSO₄) and concentrated in vacuo to an oil (0.9g). The oil was purified by chromatography over silica gel eluting with a gradient of methanol/dichloromethane to afford an orange oil (0.28g, Rf 0.11, methanol/dichloromethane 1:19). This material was further purified by passage through a strong cation exchange (SCX) column eluting firstly with methanol (fractions discarded) and then with methanol/aqueous ammonia-880 (10:1) to give the title compound (Ref E1) as an orange oil (0.152g, 0.41mmol, 7% over two steps); δ_H (CDCl₃) 2.40 (3H, s), 2.72-2.76 (4H, m), 3.44 (4H, br, s), 7.25-7.27 (1H, m), 7.43-7.61 (5H, m), 7.99-8.02 (2H, m), 8.75 (1H, d, J = 2.4Hz), 9.21 (1 H, d, J = 2.4Hz); Mass Spectrum : C₂₀H₂₁N₃O₂S requires 367; found 368 (MH⁺).

Reference Example 1 (Alternative Procedure 1) 8-(4-Methyl-piperazin-1-yl)-3-phenylsulfonylquinoline (Ref E1)

A solution of 8-amino-3-phenylsulfonylquinoline (D5) (38.8 g, 137 mmol) in t-butanol (360 ml) was treated with bis-(2-chloroethyl)-methyl-amine hydrochloride (40 g, 138 mmol) and sodium carbonate (72 g, 0.68 mol). The mixture was heated to a vigorous reflux (~100 °C) for 16 h then a further portion of bis-(2-chloroethyl)amine hydrochloride (25 g, 86 mmol) introduced and heating continued for a further 4 h. The solution was cooled and a 1:1 mixture of saturated aqueous sodium bicarbonate and aqueous 10% sodium thiosulphate solution (2 L) added. Stirring was continued at ambient temperature for 16 h then the aqueous phase was extracted with dichloromethane (3 x 500 ml), the combined organic phase dried over magnesium sulphate, evaporated in vacuo and subjected to chromatography on a Biotage Flash 75 apparatus (1 kg Silica gel) to afford the title compound (Ref E1) as the free base form (11.6 g), identical spectroscopically to that prepared by the first method. A portion of this material was treated with 1 M HCl in ether then evaporated to afford the hydrochloride salt as a yellow solid; δ_H (CDCl₃) 2.95 (3H, d), 2.38-3.52 (4H, m), 4.01-4.06 (2H, m), 4.19-4.26 (2H, m), 7.60 (2H, t), 7.70 (1H, t), 7.96 (1H, t), 8.07 (2H, s), 8.09 (2H, s), 9.34 (1H, d), 9.63 (1H, d), 12.9 (1H, br s)

Reference Example 1 (Alternative Procedure 2) 8-(4-Methyl-piperazin-1-yl)-3-phenylsulfonylquinoline (Ref E1)

A suspension of 3-phenylsulfonyl-8-piperazin-1-yl-quinoline (200 mg 0.55 mmol) ir ethanol (4 ml) was treated successively with acetic acid (100µl), 37% formaldehyde in aqueous methanol (formalin) (0.1 ml) and resin bound Amberlyst cyanoborohydride (∼3 mmol/g, 0.5g). The mixture was stirred at ambient temperature for one hour then filtered and the filtrate absorbed onto an SCX cartridge. This was washed with ethanol then eluted with a solution of 3% ammonia in 7% aqueous methanol. The solution was evaporated and the residue subjected to purification by flash chromatography on silica gel (eluting with dichloromethane - methanol - aq. NH₃) to give the title compound (Ref E1), identical spectroscopically to that prepared by the earlier methods.

Reference Example 1 (Alternative procedure 3) 8-(4-Methyl-piperazin-1-yl)-3-phenylsulfonylquinoline (Ref E1)

8-lodo-3-phenylsulfonyl-quinoline (D6) (190 mg, 0.48 mmol), N-methyl-piperazine (48 mg, 0.48 mmol), sodium tertbutoxide (65 mg, 0.68 mmol), di-palladium tetrakis-(dibenzylidine acetone) [Pd₂(dba₃] (88 mg, 0.1 mmol) and 1,1'-diphenylphosphino ferrocene (161 mg, 0.3 mmol) were suspended in degassed dry dioxan (2 ml). The mixture was stirred under argon at 40°C for 16 hours. The solvent was removed and the residue subjected to flash chromatography on silica gel (eluting with dichloromethane - methanol aq. ammonia) to give the title compound (Ref E1), identical spectroscopically to that prepared by the earlier methods.

Example 1 3-Phonylsulfonyl-8-piperazin-1-yl-quinoline hydrochloride (E1)

A stirred solution of 8-(4-methyl-piperazin-1-yl)-3-phenylsulfonyiquinoline (Ref E1) (0.148g, 0.4mmol), 1-chloroethyl chloroformate (0.093ml, 0.85mmol) and N,N-diisopropylethylamine (0.148ml, 0.85mmol) in 1,2-dichloroethane (9ml) was heated at reflux for 1.25h under argon. The reaction mixture was cooled to ambient temperature and concentrated in vacuo to an oil. The oil was purified by chromatography over silica gel eluting with a gradient of methanol/dichloromethane, pooling fractions which contained the major component (Rf 0.9, methanol/dichloromethane 1:19). The purified material was redissolved in methanol (15ml) and the solution was refluxed for 1 h under argon. The reaction mixture was cooled to ambient temperature and concentrated in vacuo to a solid which was stirred with diethyl ether (5ml) and filtered to afford the title compound (E1) (0.08g 0.21 mmol, 51%); δ_H (d₆-DMSO) 3.32 (4H, br s), 3.55 (4H, br s), 7.35 (1 H, d, J = 6.5Hz), 7.63-7.77 (4H, m), 7.86 (1H, d, J = 7.4Hz), 8.10 (2H, m), 9.10 (1H, d, J = 2.4Hz), 9.21 (2H, s), 9.24 (1 H, d, J = 2.4Hz); Mass Spectrum : C₁₉H₁₉N₃ O₂S requires 353; found 354 (MH⁺).

Example 1 (Alternative Procedure) 3-Phenylsulfonyl-8-piperazin-1-yl-quinoline hydrochloride (E1)

A mixture of 8-(4-t-butoxycarbonyl)piperazin-1-yl-3-phenylsulfonylquinoline (D7) (35.7 g, 78.8 mmol), 1,4-dioxane (200 ml) and 4 M aqueous HCl (200 ml), was stirred a ambient temperature for two hours, then the solvent evaporated. The residue was co-evaporated several times from toluene and the remainder crystallised from hot ethanol to give the title compound (E1) (18.9 g, 68%) as a yellow crystalline solid; δ_H (d₆-DMSO) 3.32 (4H, br s), 3.55 (4H, br s), 7.35 (1H, d, J = 6.5Hz), 7.63-7.77 (4H, m), 7.86 (1H, d, J = 7.4Hz), 8.10 (2H, m), 9.10 (1H, d, J = 2.4Hz), 9.21 (2H, s), 9.24 (1H, d, J = 2.4Hz); Mass Spectrum : C₁₉H₁₉N₃O₂S requires 353; found 354 (MH⁺); m.p. 200°C (phase change), 270-274°C (decomposed)

Example 2 3-Phenylsulfonyl-8-piperazin-1-yl-quinoline (E2), (free base form of E1)

A 100 ml three necked flask was charged with Pd₂(dba)₃ (174 mg, 0.19 mmol, 0.03eq), 8-lodo-3-phenylsulfonylquinoline (D6) (2.5g, 6.33 mmol), 1,1'-bis-diphenylphosphenoferrocene (316 mg, 0.57 mmol), sodium tertbutoxide (851 mg, 8.86 mmol, 1.4eq) and piperazine (2.72g, 31.6 mmol, 5eq). The flask was evacuated and filled with nitrogen 4 times then anhydrous 1,4-dioxane (17.5 ml, 7vol) was added. The mixture was stirred and heated to 40°C for 16½ hrs. The dark solution was allowed to cool to room temperature, dichloromethane (12.5 ml) was added and the solution was washed with H₂O (12.5 ml). The aqueous wasn was extracted with dichloromethane and the combined organic layers were extracted with 5M HCl (2x12.5 ml). The combined aqueous layers were washed with (dichloromethane 2.5 ml) then transferred to a conical flask, dichloromethane (12.5 ml) was added and the flask was cooled in an ice/water bath. 10M Aqueous sodium hydroxide (13 ml) was added whilst stirring, the mixture was then stirred at room temperature until all the solids were dissolved. The lower organic layer was removed and the aqueous layer was extracted with dichloromethane (7.5 ml), the combined organic layers were concentrated under reduce pressure to -5 ml. Isooctane (2.5 ml) was added to the dark brown solution resulting in crystallisation, the mixture was stirred at room temp for 5 min then isooctane (22.5 ml) was added over 5 min. The mixture was aged at room temp for 1½ hrs before being cooled in an ice/water bath for 30 min, the mixture was filtered and the cake washed with isooctane (5 ml). The cake was dried under reduced pressure to give the title compound E2; yield 1.67g, 75%. δ_H (CDCl₃): 1.6 (1H, bs), 3.18 (4H, m), 3.34 (4H, m), 7.27 (1H, m), 7.49-7.60 (5H, m), 8.01 (2H, dd), 8.75, (1H, d), 9.21 (1H, d).

Example 3 Crystallisation of 3-phenylsulfonyl-8-piperazin-1-yl-quinoline (Form I)

3-Phenylsulfonyl-8-piperazin-1-yl-quinoline (0.1g) was dissolved in ethyl acetate (1.7ml) with warming. On cooling the product crystallised as needles. Solvent was allowed to evaporate to afford the title compound in quantitative recovery. Melting point 158°C.

Characterising data recorded for Form I:

The infrared spectrum of the solid product was recorded using a Nicolet Avatar 360 FT-IR spectrometer fitted with a universal ATR accessory. The FT-IR spectrum (Figure 1) shows bands at: 2945, 2819, 1606, 1590, 1566, 1487, 1469, 1447, 1380, 1323, 1283, 1247, 1164, 1138, 1126, 1107, 1095, 1083, 1056, 1026, 997, 964, 949, 919, 906, 879, 859, 824, 785, 761, 723, 705 cm^-1

The FT-Raman spectrum was acquired using a ThermNicolet 960 E.S.P. spectrometer. Excitation at 1064 nm was provided by a Nd:YVO₄ laser with a power of 400 mW at the sample position. 1200 scans were recorded at 4 cm^-1 resolution. The FT-Raman spectrum (Figure 2) shows bands at 215, 252, 293, 304, 315, 338 556, 705, 858, 997, 1025, 1098, 1154, 1363, 1382, 1397, 1566, 1584, 1606 and 3059 cm^-1.

The X-Ray Powder Diffractogram pattern of the product (Figure 3) was recorded using the following acquisition conditions: Unground material was packed into top-filled Si cups. Powder patterns were obtained using a Bruker D8 Advance X-Ray powder diffractometer configured with a Cu anode (40 kV, 40 mA), variable divergence slit, primary and secondary Soller slits, and a position sensitive detector. Data were acquired over the range 2 - 40 degrees 2-theta using a step size of 0.0145 degrees 2-theta (1 s per step). Samples were rotated during data collection. Characteristic 2θ XRPD angles are 6.84, 8.61, 10.47, 13.01, 15.11, 15.50, 16.24, 16.63, 17.20, 18.00, 19.65, 21.07, 21.66, 22.20, 22.62, 23.99, 25.61, 26.12, 26.76, 27.96, 28.86, 29.64, 30.26, 30.85, 31.31, 32.60, 33.08, 33.70, 34.35, 35.65, 36.85, 38.05 and 38.46°.

Example 4 Crystallisation of 3-phenylsulfonyl-8-piperazin-1-yl-quinoline (Form II)

3-Phenylsulfonyl-8-piperazin-1-yl-quinoline (0.5g) was dissolved in isopropanol (5ml) with warming. The solution was allowed to cool to ambient then stirred overnight before cooling in an ice-water bath for 15 min. The product was collected by filtration, and dried in vacuo at 50°C to give the title compound, 371mg, 74%. Melting point 164°C.

Characterising data recorded for Form II:

The infrared spectrum was recorded using a Nicolet Avatar 360 FT-IR spectrometer fitted with a universal ATR accessory. The FT-IR spectrum (Figure 4) shows bancs at: 3335, 2939, 2812, 1585, 1564, 1485, 1470, 1443, 1382, 1361, 1322, 1310, 1250, 1232, 1179, 1158, 1129, 1107, 1093, 1061, 1022, 1000, 950, 914, 862, 813, 774, 760, 727 cm^-1

The FT-Raman spectrum of a sample in a glass tube was acquired using a ThermNicolet 960 E.S.P. spectrometer. Excitation at 1064 nm was provided by a Nd:YVO₄ laser with a power of 400 mW at the sample position. 1200 scans were recorded at 4 cm^-1 resolution. The FT-Raman spectrum (Figure 5) shows bands at 216, 252, 288, 617, 701, 726, 863, 1000, 1026, 1078, 1153, 1197, 1339, 1360, 1331, 1396, 1445, 1564, 1584, and 3052 cm^-1. The X-Ray Powder Diffractogram pattern (Figure 6) was recorded using the following acquisition conditions: Unground material was packed into top-filled Si cups. Powder patterns were obtained using a Bruker D8 Advance X-Ray powder diffractometer configured with a Cu anode (40 kV, 40 mA), variable divergence slit, primary and secondary Soller slits, and a position sensitive detector. Data were acquired over the range 2 - 40 degrees 2-theta using a step size of 0.0145 degrees 2-theta (1 s per step). The sample was rotated during data collection. Characteristic 2θ XRPD angles are 9.30, 9.95, 10.99, 13.40, 14.63, 15.03, 16.04, 16.47, 17.93, 18.19, 18.73, 19.17, 20.69, 21.49, 22.12, 23.55, 24.59, 25.27, 27.03, 28.22, 28.61, 29.48, 29.81, 30.70, 32.05, 33.32, 33.95, 34.39, 34.90, 35.77, 36.25, 36.80, 37.60, 38.19, 38.70 and 39.26°.

Pharmacological data

Compounds can be tested following the procedures outlined in WO98/27081 . The compounds of Examples E1 and E2 were tested and showed good affinity for the 5-HT₆ receptor, having pKi values > 8.0 at human cloned 5-HT₆ receptors.

Brief Description of the Drawings

• Figure 1 shows the Infrared spectrum obtained for 3-phenylsulfonyl-8-piperazin-1-yl-quinoline (Form I).
• Figure 2 shows the Raman spectrum obtained for 3-phenylsulfonyl-8-piperazin-1-yl-quinoline (Form I).
• Figure 3 shows the X-Ray Powder Diffractogram obtained for 3-phenylsulfonyl-8-piperazin-1-yl-quinoline (Form I).
• Figure 4 shows the Infrared spectrum obtained for 3-phenylsulfonyl-8-piperazin-1-yl-quinoline (Form II).
• Figure 5 shows the Raman spectrum obtained for 3-phenylsulfonyl-8-piperazin-1-yl-quinoline (Form II).
• Figure 6 shows the X-Ray Powder Diffractogram obtained for 3-phenylsulfonyl-8-piperazin-1-yl-quinoline (Form II).

Claims (20)

1. A compound of formula (I) or a pharmaceutically acceptable salt thereof: wherein:

   R¹ and R² represent hydrogen;

   R³, R⁴ and R⁵ represent hydrogen;

   m represents 1;

   n represents 1;

   p represents 1;

   A represents phenyl; or a solvate thereof.
2. A compound of formula (I) according to claim 1 which is 3-phenylsulfonyl-8, piperazin-1-yl-quinoline or a pharmaceutically acceptable salt thereof.
3. A compound of formula (I) according to claim 1 or 2 which is the free base of 3-phenylsulfonyl-8-piperazin-1-yl-quinoline.
4. A compound of formula (I) according to claim 3 which is 3-phenylsulfonyl-8-piperazin-1-yl-quinoline (Form I).
5. A compound of formula (I) according to claim 3 which is 3-phenylsulfonyl-8-piperazin-1-yl-quinoline (Form II).
6. A process for the preparation of a compound of formula (I) as defined in any one of claims 1 to 5 or a pharmaceutically acceptable salt or solvate thereof, which comprises:

   (a) reacting a compound of formula (II) wherein R^1a is as defined for R¹ in claim 1 or an N-protecting group, R², R³, R⁴, R⁵, m, n and p are as defined in claim 1 and L¹ is a leaving group; with a compound of formula A-SO₂H, (or A-SH followed by a subsequent oxidation step) wherein A is as defined above and thereafter as necessary removing an R^1a N-protecting group; or

   (b) reacting a compound of formula (IV) with a compound of formula (V) wherein R^1a, R², R³, R⁴, R⁵, A, m, n and p are as defined in claim 1, and L² represents a suitable leaving group, and thereafter as necessary removing an R^1a N-protecting group; or

   (c) reacting a compound of formula (VI) with a compound of formula (VII) wherein R^1a, R², R³, R⁴, R⁵, m, n, p and A are as defined in claim 1 and L³ represents a suitable leaving group, and thereafter as necessary removing an R^1a N-protecting group; or

   (d) deprotecting a compound of formula (I) which is protected; and thereafter optionally

   (e) interconversion of another compound of formula (Ia)

   wherein:

   R¹ and R² independently represent hydrogen or C_1-6 alkyl or R¹ is linked to R² to fo-m a group (CH₂)₂, (CH₂)₃ or (CH₂)₄;

   R³, R⁴ and R⁵ independently represent hydrogen, halogen, cyano, -CF₃, -CF₃O, C_1-6 alkyl, C_1-6 alkoxy, C_1-6 alkanoyl or a group -CONR⁶R⁷;

   R⁶ and R⁷ independently represent hydrogen or C_1-6 alkyl or together may be fused to form a 5- to 7- membered aromatic or non-aromatic heterocyclic ring optionally interrupted by an O or S atom;

   m represents an integer from 1 to 4, such that when m is an integer greater than 1 two R² groups may instead be linked to form a group CH₂, (CH₂)₂ or (CH₂)₃;

   n represents an integer from 1 to 3;

   p represents 1 or 2;

   A represents a group -Ar¹ or - Ar²Ar³;

   Ar¹, Ar² and Ar³ independently represent an aryl group or a heteroaryl group, both of which may be optionally substituted by one or more (eg. 1, 2 or 3) substituents which may be the same or different, and which are selected from the group consisting of halogen, hydroxy, cyano, nitro, trifluoromethyl, trifluoromethoxy, C_1-6 alkyl, trifluoromethanesulfonyloxy, pentafluoroethyl, C_1-6 alkoxy, arylC_1-6 alkoxy, C_1-6 alkylthio, C_1-6 alkoxyC_1-6 alkyl, C_3-7 cycloalkylC_1-6 alkoxy, C_1-6 alkanoyl, C_1-6 alkoxycarbonyl, C_1-6 alkylsulfonyl, C_1-6 alkylsulfinyl, C_1-6 alkylsulfonyloxy, C_1-6 alkylsulfonylC_1-6 alkyl, arylsulfonyl, arylsulfonyloxy, arylsulfonylC_1-6 alkyl, C_1-6 alkylsulfonamido, C_1-6 alkylamido, C_1-6 alkylsulfonamidoC_1-6 alkyl, C_1-6 alkylamidoC_1-6 alkyl, arylsulfonamido, arylcarboxamido, arylsulfonamidoC_1-6 alkyl, arylcarboxamidoC_1-6 alkyl, aroyl, aroylC_1-6 alkyl, arylC_1-6 alkanoyl, or a group CONR⁸R⁹ or SO₂NR⁸R⁹, wherein R⁸ and R⁹ independently represent hydrogen or C_1-6 alkyl or together may be fused to form a 5- to 7- membered aromatic or non-aromatic heterocyclic ring optionally interrupted by an O or S atom; and/or forming a pharmaceutically acceptable salt and/or solvate.
7. A pharmaceutical composition which comprises a compound according to any one of claims 1 to 5 thereof and a pharmaceutically acceptable carrier or excipient
8. A pharmaceutical composition which comprises 3-phenylsulfonyl-8-piperarn-1-yl-quinoline and a pharmaceutically acceptable carrier or excipient.
9. A compound according to any one of claims 1 to 5 for use in therapy.
10. A compound according to any one of claims 1 to 5 for use in the treatment of anxiety, depression, epilepsy, obsessive compulsive disorders, migraine, cognitive memory disorders, Parkinsons Disease, ADHD (Attention Deficit Disorder/Hyperactivity Syndrome), sleep disorders, feeding disorders, panic attacks, withdrawal from drug abuse, schizophrenia, stroke, disorders associated with spinal trauma and/or head injury, Irritable Bowel Syndrome or obesity.
11. A compound according to claim 10 wherein the cognitive memory disorder is selected from Alzheimers disease, age related cognitive decline and mild cognitive impairment.
12. A compound according to any one of claims 1 to 5 for use in the treatment of depression, anxiety, Alzheimers disease, age related cognitive decline, ADHD, obesity, mild cognitive impairment, schizophrenia, cognitive deficits in schizophrenia and stroke.
13. A compound according to any one of claims 1 to 5 for use in the treatmen: of Alzheimers disease.
14. 3-Phenylsulfonyl-8-piperazin-1-yl-quinoline for use in the treatment of Alzheimers disease.
15. Use of a compound as defined in any one of claims 1 to 5 or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for the treatment or prophylaxis of anxiety, depression, epilepsy, obsessive compulsive disorders, migraine, cognitive memory disorders, Parkinsons Disease, ADHD (Attention Deficit Disorder/Hyperactivity Syndrome), sleep disorders, feeding disorders, panic attacks, withdrawal from drug abuse, schizophrenia, stroke, disorders associated with spinal trauma and/or head injury, Irritable Bowel Syndrome or obesity.
16. The use of according to claim 15 wherein the cognitive memory disorder is selected from Alzheimers disease, age related cognitive decline and mild cognitive impairment.
17. Use of a compound as defined in any one of claims 1 to 5 or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for the treatment or prophylaxis of depression, anxiety, Alzheimers disease, age related cognitive decline, ADHD, obesity, mild cognitive impairment, schizophrenia, cognitive deficits in schizophrenia and stroke.
18. Use of a compound as defined in any one of claims 1 to 5 or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for the treatment or prophylaxis of Alzheimers disease.
19. Use of 3-phenylsulfonyl-8-piperazin-1-yl-quinoline or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for the treatment or prophylaxis of Alzheimers disease.
20. Use of a compound as defined in any one of claims 1 to 5 in the manufacture of a medicament for promoting neuronal growth within the central nervous system of a mammal