HK1060834B - Composition and method for smoke detoxification - Google Patents

Description

Composition and method for smoke detoxification

no marking

This application claims the benefit of U.S. provisional application 60/267,428 filed on 9/2/2001.

Technical Field

The present invention relates generally to methods for achieving smoke detoxification. The invention particularly relates to turmeric-containing compositions for effecting smoke detoxification.

Background

Tobacco smoke derived from smoldering tobacco or exhaled by active smokers is a source of exposure of humans to mutagens and carcinogens. Studies have shown a close correlation between exposure to tobacco smoke and health hazards such as respiratory and pulmonary heart diseases and lung cancer in smokers and non-smokers. Active and passive smokers are known to excrete large amounts of tobacco-derived mutagens in their urine. In addition, studies have identified a correlation between human cancer and overcooked meat, such as, for example, meat that is cooked on a grill. (see Burros, m., "tea. Creatine in muscle reacts with amino acids at high temperatures to form cancer causing compounds called heterocyclic amines. However, salt brine pickling has been used to reduce these heterocyclic amines. Scientists speculate that antioxidants such as garlic, onions, chives, turmeric, thyme, rosemary, and oregano, as well as vitamins C and E, help to reduce such amines. However, when fat is put on a fire and smoke is produced, salting does not reduce the level of other carcinogens rising. (see the Burros article).

Turmeric has been found to be effective in inhibiting the formation and excretion of urinary mutagens in smokers. (see P.Kalpagam, T.C.Raghuram, T.P.Krishna and K.Krishnaswamy, "Effect of turmeric on Urinary Mutagens in Smokers" ("effective of turmeric on tobacco Mutagens in Smokers"), "Mutagenesis" (Mutagens), Vol.7, No. 2, p.107. sup. 109 (1992) (which are believed to detoxify tobacco Mutagens with the active ingredient curcumin.) Curcuma has also been found to be an effective antimutagen and can be used for chemoprevention. Krishnaswamy, K., and Raghuramulu, N., "Bioactive Phytochemicals with significant impact on Dietary practice" (Bioactive Phytochemicals on diet Practices), "journal of Indian drug research (Indian J Med Res)108, 11 months 1998, pp.167-181; deshopande, s.s., inge, a.d., and Maru, g.b., "inhibition of Benzo [ α ] pyrene-Induced forestomas in Mice by Curcumin-Free Aqueous turmeric extract" (inhibition of Cancer-Free Aqueous tumor extract on Benzo [ alpha ], "Cancer communication, 118(1997) 79-85; srimal r.c, "turmeric: review of drug Properties "(Turmeric: A Brief Review of Medicinal Properties), Fitoteappaia, Vol.LXVIII, No. 6, 1997, pp.483-493; arbiser, j.l., Klauber, n., Rohan, r., van leewen, r., huangng, m.t., Fisher, c., Flynn, e., Byers, h.r., Curcumin is an In Vivo Inhibitor of angiogenesis (currin an Vivo Inhibitor of angiogenesis), "molecular medicine" (Mol Med) (1998, 6 months), 4 (6): 376 to 83; plummer, s.m., Holloway, k.a., Manson, m.m., Munks, r.j., Kaptein, a., Farrow, s., and Howells, l., "chemopreventive Curcumin inhibits cyclooxygenase 2 Expression in Colon Cells including Inhibition of NF- κ B activation by the NIK/IKK Signaling Complex" (Inhibition of Cyclo-Oxygenase 2 Expression in colons Cells by the chemopreventive Agent current receptor of NF-kappaB activation Via the NIK/IKK Signaling Complex), "Oncogene (Oncogene) (1999, 28 th.10, 18 (44): 6013-20; singhal, s.s., Awasthi, s., Pandya, u., pip, j.t., Saini, m.k., Cheng, j.z., and Awasthi, y.c., "The Effect of curcumin on Glutathione-Linked Enzymes in K562 Human leukemia cells" (The Effect of currington Glutathione-Linked Enzymes in K562 Human leukemia cells), "toxicology communications" (Toxicol Lett)1999, 9/20/109 (1-2): 87-95; kang, b.y., Song, y.j., Kim, k.m., Choe, y.k., Hwang, s.y., Kim, t.s., "Curcumin Inhibits the distribution of Th1 Cytokine in CD4+ T Cells by inhibiting the Production of Interleukin-12 in Macrophages" (economy in inhibition Th1 Cytokine Profile in CD4+ T Cells bysupressing Interleukin-12 genes), "british journal of pharmacology (Br J Pharmacol), 9 months 1999, 128 (2): 380-4.

In addition, turmeric may be used to reduce the sharply increased fibrinogen levels. (see Ramirez-Bosca A., Soler A., Carrion-Gutierrez, M.A., Mira D.P., Zapata J.P., Diaz-Alperi J., Bernd A., Almagro E.Q., and Miquel, J., "correlation of hydroalcoholic extracts of Curcuma rhizome to reduce the ratio of Apo B/Apo A for atheroprotection" (A Hydrocellulosic extract of Curcuma Longa Lowers the Apo B/Apo A ratios for atherogenicity) aging and Development mechanism (Mechanisms of aging and Development)114(2000) 207-210. Curcuma rhizome appears to be beneficial to the cardiovascular system of persons exposed to tobacco as such.

It has been reported that eugenol, a compound present in many flavors such as clove, cardamom, and the like, exhibits resistance to mutagenicity to tobacco smoke. (see Sukumuran K. and Ramadasan K., "suppression of Tobacco-Induced Mutagenesis with Eugenol and plant Extracts" (Inhibition of suppression of Tobacco-Induced Mutagenesis by Eugenol and plant Extracts), "Mutagenesis Research (Mutation Research)343(1995) 25-30). In addition, eugenol is believed to exhibit anti-peroxidation activity. (see Krishnaswamy, K. and Raghuramulu, N., "Bioactive Phytochemicals with significant impact on Dietary practice" (Bioactive Phytochemicals on diet Practices), "Indian journal of drug research (Indian J Med Res)108, 11 months 1998, pp.167-181).

Green tea is reported to have chemopreventive effects on cigarette smoke-induced mutations in humans. (see Lee I.P., Kim Y.H., Kang M.H., Roberts C., Shim J.S., and Roh J.K., "Chemopreventive Effect of Green Tea (Pu' er Tea) on Smoke-Induced Mutations in Humans (SCE) in cigarettes" (Chemopreventive Effect of Green Tea (Camellia sinensis) agricultural magic Patton Ref., 9510.101 Smok-Induced Mutations (SCE) in Humans), "Journal of cell Biochemistry augmentation (Journal of Cellular Biochemistry Supplement) 27: 68-75 (1997)). See, for example, Klaunig j.e., Xu y., Han c., Kamendulis l.m., Chen j., Heiser c., Gordon m.s., and Mohler III e.r., "Effect of Tea drinking on oxidative stress in Smokers and non-Smokers" (The Effect of Tea treatment on oxidative stress in cookers and Nonsmokers), "Antioxidant Effect of Tea" (Antioxidant Effects of Tea), pp.249-254 for a discussion of The Effect of drinking green Tea on smoking-induced oxidative damage. For example, see Renqing Z, Zhouy, Chen D, Shenben L, and Haug A, "Effect of Soaking Temperature and Soaking time in the Preparation of aqueous Tea extracts on Environmental Tobacco Smoke resistance in Sister chromosome Exchange tests" (Effects of Soaking Temperature and Soaking time preference of Water Extract of Tea on Environmental Tobacco Smoke inter-site-Chromatid Exchange Assay), "Toxicology communications (Toxicology Letters)115(2000)23-32 for discussion of the anti-inducing activity of green Tea aqueous extracts on Environmental Tobacco Smoke. Furthermore, green tea has been reported to exhibit cancer chemopreventive effects. (see Katiyar K.S., Agarwal R., Zaim M.T., and Mukhtar H., "Preventive action of Green tea on forestomach and Lung Carcinogenesis in N-nitrosodiethylamine and Benzo [ alpha ] pyrene-induced A/J Mice" (Protection agenet N-nisodiethylamide and Benzo [ alpha ] pyridine-induced molding and Long pulmonary oncogenesis in A/J mix by GreenTea), "Carcinogenesis" (Carcinogenesis), volume 14, phase 5, pp.849-855 (1993); Suganuma M., Okabe S., Kai Y., Suveoka N., Sueoka gall, Fujiki, Preh., "Synergistic action of Epepicatechin ester with Epicatechin, Sulindac or Synechol Cancer Cell Line 9, (" Preventive action of Cancer on Lung Cancer Cell Line 9, Cancer Cell Line (-) -fashion "),9-), cancer Research 59, 44-47, 1/1999.

It is reported that cigarette filters containing a specific galenical formulation of rosemary extract are effective in reducing the free radicals found in smoke. (see I.Emani, C.Rolando, M.Rojas, K.Alexandrov, H.Scherf, and H.Bartsch: "Rosemary Cigarette filters can reduce Tobacco-related Cancer" (A Rosemary Cigarete Filter Tobacco MayReduce Tobacco-Linked Cancer), "biosynthesis and Chemopreventive filtration" (Biosyntech Chemopreventive Filter-Coresta, pp.3-10, 10 months 2000).

The volatile aromatic component, myristyl ether, in parsley leaf oil was reported as a possible cancer chemopreventive agent. (see Zheng G., Kenney P.M., Zhang J., and Lam L.K.T., "Inhibition of Benzo [ alpha ] pyridine-induced Carcinogenesis by Benzo [ alpha ] pyrene by Myristicin, a Volatile aromatic component in Parsley Leaf Oil," (Carcinogenesis of Parsley Leaf Oil), "Carcinogenesis (Carcinogenesis), Vol.13, No. 10, pp.1921-1923 (1992)).

Thus, it is known to use natural components to detoxify tobacco. However, there is a need for improved smoke detoxification activity using a combination of natural components.

SUMMARY

One embodiment of the present invention provides a method for effecting smoke detoxification in humans by using a composition consisting of effective amounts of a supercritical extract and a hydroalcoholic extract of turmeric.

Detailed Description

One embodiment of the present invention provides a composition for effecting smoke detoxification in humans. The composition may contain effective amounts of supercritical and hydroalcoholic turmeric extracts. The composition may also contain effective amounts of the following components: (A) supercritical and hydroalcoholic ginger extracts; (B) supercritical extracts of rosemary, parsley seed, peppermint and clove; (C) hydroalcoholic extracts of rosemary, parsley leaf, peppermint and clove; and (D) an aqueous extract of green tea. For example, the composition may provide improved smoke detoxification properties over known compositions because it contains turmeric extract prepared by a supercritical/hydroalcoholic double extraction process. On the other hand, known compositions contain only turmeric extract prepared using only one of the supercritical extraction method or the hydroalcoholic extraction method.

Another embodiment of the present invention provides a method of effecting smoke detoxification in a human comprising the step of administering (orally) to a human exposed (directly or indirectly) to a toxin associated with a form of smoke, such as a toxin from tobacco or other partially combustible hydrocarbon source, an effective amount of the composition for a therapeutically effective period of time. As such, the composition may be administered for a period of time sufficient to effect smoke detoxification in a human.

The (herbal) composition (excluding inactive ingredients) may be administered orally in a daily dose of at least about 350mg or about 375-2000 mg. If the composition includes inactive ingredients, the active ingredients (e.g., herbal extracts) of the composition may include amounts commonly used in orally administered compositions. For example, the composition may be administered on a daily basis for a period of at least 4 weeks. For example, oral administration may be carried out by swallowing the composition with water. The compositions for oral administration may be in any conventional form, including, for example, capsules (hard and/or soft), tablets, elixirs, powders, granules, suspensions in aqueous or non-aqueous media, sachets, and the like. The orally administered composition may also be in the form of one or more soft gelatin capsules.

The term "smoke detoxification" is meant to include reducing elevated levels of mutagens and fibrinogen in a human. Elevated levels of such substances may result from direct or indirect human contact with smoke, for example from tobacco, hydrocarbon combustion, barbeque, etc.

The turmeric extract may be a "full range" extract of the herb, such that the extract may contain curcumin as well as oils and other ingredients that provide improved smoke detoxification activity. As such the turmeric extract may contain not only curcuminoid fractions but also naturally occurring protein antioxidant factors and valuable essential oil components such as turmerone. However, conventional turmeric extracts are only prepared by isolating one component, particularly one or more curcuminoids, by solvent extraction (e.g., using acetone or methylene chloride).

Thus, the turmeric extract may contain a supercritical extract of the lipophilic fraction of the plant and a hydroalcoholic extract of the hydrophilic fraction of the plant. The use of supercritical extraction allows the lipophilic components not to be degraded during the extraction process by the pressure of the solvent, oxygen and/or heat as occurs in conventional extraction. The lipophilic component can thus be used in the purest and most concentrated form. As such, the turmeric extract may provide the consumer with the opportunity to experience the recovery and detoxification properties of turmeric in its most complete form.

Turmeric extract includes unique clearing and regulating capabilities, especially it may have a synergistic effect with green tea water extract. Green tea water extract itself did not exhibit significant prophylactic activity against known mutagens. Mint and clove extracts are also inhibitors of cigarette and other smoke mutagenicity. The rosemary and parsley leaf extracts enhance (make them uniform) detoxification activity and enhance the efficacy of the composition. The ginger extract has activity on benzopyrene and tryptophan pyrolysates and improves the bioavailability of the composition.

The supercritical extract and post-supercritical hydroalcoholic extract of turmeric may be prepared as follows. Turmeric root, which may be ground at low temperatures to protect heat sensitive ingredients, may be subjected to supercritical extraction to obtain (i) an oil extract containing precisely lipophilic (e.g., oil soluble/non-polar) ingredients (hereinafter "supercritical turmeric extract") and (ii) an oil-free residue. Suitable supercritical Extraction processes that can be used to obtain supercritical turmeric extracts are disclosed, for example, in "high density gas for Extraction and Refining" (DenseGases for Extraction and Refining), Springer Verlag 1988, by E.Stahl, K.W.Quirin, D.Gerard, which is incorporated herein by reference.

It has been found that about 25-33 kg of turmeric meal can produce 1 kg of oil extract. The oil-free residue can then be extracted with a water/alcohol (e.g., water/ethanol) mixture consisting of 60-80 parts alcohol and 40-20 parts water. Extraction of the oil-free residue with this water/alcohol mixture results in a wide range of polar constituents, including water-soluble constituents and curcuminoids (e.g., the full range of curcuminoids). The water/alcohol liquid can then be evaporated off, resulting in a powdery extract residue, hereinafter referred to as "hydroalcoholic turmeric extract (after supercritical extraction"). It has been found that about 6 kg of oil-free turmeric residue may yield about 1 kg of post-supercritical hydroalcoholic turmeric extract. The post-supercritical hydroalcoholic extract may be combined or blended with the supercritical turmeric extract in a weight ratio of about 3.0-6.0 parts post-supercritical hydroalcoholic extract to 1 part supercritical turmeric extract. The post-supercritical hydroalcoholic extract and the supercritical extract may also be combined in a weight ratio of about 5.3 parts post-supercritical hydroalcoholic extract per 1 part supercritical turmeric extract. The supercritical turmeric extract and the hydroalcoholic turmeric extract of the supercritical extract may be added separately and blended with the other extracts, provided that the resulting composition contains the supercritical turmeric extract and the hydroalcoholic turmeric extract after the supercritical extraction in appropriate weight ratios.

The supercritical extract and post-supercritical hydroalcoholic extract of ginger may be prepared using the procedures used to prepare the supercritical extract and post-supercritical hydroalcoholic extract of turmeric. The supercritical extract of ginger and the hydroalcoholic extract after supercritical extraction may be blended with each other and then added to other herbal extracts. The resulting composition may contain a hydroalcoholic post-supercritical ginger extract and a supercritical ginger extract, both of which may be combined in a weight ratio of about 4.4 parts post-supercritical extract to 1 part supercritical extract.

In addition, the supercritical extracts of clove, peppermint, parsley seed and ambient aroma can be prepared using the procedure used to prepare the supercritical extracts of turmeric and ginger. Hydroalcoholic extracts of clove, peppermint, parsley leaf and rosemary, respectively, may be prepared by extracting the plant parts with a water/alcohol (e.g., water/ethanol) mixture consisting of 60-80 parts alcohol and 40-20 parts water. The water/alcohol liquid can then be evaporated off, resulting in a powdery extract residue. For each of clove, peppermint, parsley and rosemary, the supercritical and hydroalcoholic extracts may be blended with each other and then added to the other herbal extracts or the supercritical and hydroalcoholic extracts may be added separately to and blended with the other herbal extracts used in the present composition.

The composition may contain the following components in a range of weight ratios: (i) (ii) a hydroalcoholic rosemary extract to a supercritical rosemary extract from about 1.75: 1 to about 2.25: 1 or about 2: 1; (ii) (ii) a hydroalcoholic parsley leaf extract to a supercritical parsley seed extract from about 1.75: 1 to about 2.25: 1 or about 2: 1; (iii) (ii) from about 4: 1 to about 6: 1 or about 4.4: 1 of the post-supercritical hydroalcoholic and supercritical ginger extracts; (iv) (ii) a hydroalcoholic peppermint extract to a supercritical peppermint extract from about 1.75: 1 to about 2.25: 1 or about 2: 1; and/or (v) a hydroalcoholic clove extract to supercritical clove extract from about 1.75: 1 to about 2.25: 1 or about 2: 1. As described above, the post-supercritical hydroalcoholic turmeric extract and the supercritical turmeric extract may be combined in a weight ratio of about 3-6 parts post-supercritical hydroalcoholic extract to 1 part supercritical extract or may be combined in a weight ratio of about 5.3 parts post-supercritical hydroalcoholic extract to 1 part supercritical turmeric extract.

The green tea water extract may be prepared by the steps of: (i) for example, immersing the dried green tea leaves in water at a temperature of 80 ℃ or below 80 ℃ for a period of about 10 to 60 minutes; (ii) filtering the immersed tea leaves to obtain an aqueous extract; and (iii) drying the extract to obtain a (dried) solid material. Such extraction processes are described, for example, in toxicological communications 115(2000)23-32, R.Zhou et al, which is incorporated herein by reference. Another suitable extraction process for obtaining an aqueous extract of green tea is described, for example, in S.K Katiyar et al Carcinogenesis (Carcinogenesis), Vol.14, No. 5, pp.849-855(1993), which is incorporated herein by reference.

The composition may comprise the following components in weight ratio: (i) about 1: 1 to about 2: 1 or about 1.5: 1 of extracts of turmeric, rosemary, parsley, ginger, peppermint and clove with (ii) green tea extract.

The composition may also contain an effective amount (e.g., an amount such that the composition exhibits smoke detoxification properties) of each of the above-described extracts. For example, the composition may contain the following components by weight: (i) about 45% -55% or about 50% of the post-supercritical hydroalcoholic and supercritical turmeric extracts, wherein the post-supercritical hydroalcoholic and supercritical turmeric extracts may be present in a suitable weight ratio to each other (see above); (ii) from about 1.5% to 2.5% of the supercritical and hydroalcoholic rosemary extract; (iii) from about 1.5% to 2.5% or about 2.0% of the supercritical parsley seed extract and hydroalcoholic parsley leaf extract; (iv) from about 1.5% to 2.5% or about 2.0% of the post-supercritical hydroalcoholic and supercritical ginger extracts of ginger, wherein the post-supercritical hydroalcoholic and supercritical ginger extracts may be present in a suitable weight ratio to each other (see above); (v) from about 1.5% to 2.5% or about 2.0% of the supercritical and hydroalcoholic peppermint extracts; (vi) from about 1.5% to 2.5% or about 2.0% of the supercritical and hydroalcoholic clove extracts; and (vii) about 35% to 45% or about 40% of an aqueous green tea extract.

In addition, the supercritical extract of turmeric may contain about 43% to 47% or about 45% of turmerones. The post-supercritical hydroalcoholic extract of turmeric may contain a minimum of about 11% or about 11% -15% curcuminoids. The supercritical extract of ginger may contain about 28% to 32% pungent compounds and about 6% to 10% zingiberene or about 30% pungent compounds and about 8% zingiberene. The hydroalcoholic post-supercritical extraction of ginger contains about 1% to 5% or about 3% of pungent compounds. The green tea aqueous extract may contain about 43% to 47% or about 45% polyphenols. The supercritical extract of eugenol can contain from about 63% to 67% or about 65% eugenol. The supercritical extract of parsley seed may contain about 23% to 27% or about 25% myristicin. The supercritical extract of peppermint may contain about 33% to about 37% or about 35% menthol. The supercritical extract of rosemary may contain about 21% to 25% or about 23% phenolic antioxidants. The hydroalcoholic extract of rosemary may contain about 21% to 25% or about 23% phenolic antioxidants.

The composition may also contain a pharmaceutically acceptable carrier such as, for example, one or more pharmaceutically suitable: inactive excipients, carriers, diluents, adjuvants and lubricants. For example, inactive excipients, carriers, diluents, lubricants and adjuvants may include: cellulose, substituted cellulose, calcium carbonate, dicalcium phosphate, starch, lactose, modified food starches, glucose, calcium sulfate, magnesium carbonate, magnesium stearate, stearic acid, glycerol, vegetable oils, polysorbates, lecithin, silicon dioxide, food coatings, talc, croscarmellose sodium, povidone, water, and gelatin. The (active ingredient) composition may contain other inactive excipients, carriers, diluents, lubricants and adjuvants, such as, for example, those disclosed in the Handbook of Food Additives (CRC Press), which is incorporated herein by reference (to the relevant part only). Pharmaceutically acceptable carriers may be included in any conventional amount, for example for use in orally administered compositions.

The following table is a typical composition (excluding inactive ingredients) wherein the composition is administered orally, e.g., by a human. The amounts listed in the table represent the daily dosages of the listed components.

Watch (A)

Daily dosage of orally administered composition
							Supercritical extract	Ethanol extract after supercritical extraction	Ethanol extract	Aqueous extracts	Plant parts
Turmeric root tuber	30mg (45% turmerone-13.5 mg)	160mg (minimum 11% curcumin-17.6 mg)	N/A	N/A	Rhizome of Japanese apricot
						Green tea	N/A	N/A	N/A	150mg (45% polyphenols-67.5 mg)	Leaf of Chinese character
Clove incense	2.5mg (65% eugenol-1.6 mg)	N/A	5mg(10∶1)	N/A	Bud of rice
						Ginger (ginger)	1.4mg (30% pungent odor compound-0.4 mg, 8% shogarene 0.1mg)	6.1mg (3% pungent odor Compound-0.18 mg)	N/A	N/A	Rhizome of Japanese apricot
Parsley	30mg (from seed) (25% myristicin-0.6 mg)	N/A	5mg (from leaf) (8: 1)	N/A	Seeds/leaves
						Mint	2.5mg (from seed) (35% menthol-0.85 g)	N/A	5mg(4∶1)	N/A	Leaf of Chinese character
Rosemary	2.5mg (23% total phenolic antioxidant-0.58 mg)	N/A	5mg (23% total phenolic antioxidant-1.15 mg)	N/A	Leaf of Chinese character

The compositions listed in the above table may also contain inactive ingredients such as, for example, olive oil (ultra pure), maltodextrin and yellow beeswax. The composition in capsule form may further comprise gelatin, vegetable glycerin, purified water and carob. The compositions listed in the table may be in the form of a soft gel capsule, wherein the amounts listed may constitute a single serving or unit dose of the composition. The capsule may be taken using 8 ounces of water or another liquid. Furthermore, for example, if exposed to high (higher) smoke concentrations, two or more capsules of the composition may be taken per day.

The embodiments described above are provided to enable any person skilled in the art to make or use the invention. Various modifications to these embodiments are possible and the generic principles provided herein may be applied to other embodiments as well. Thus, the present invention is not intended to be limited to the embodiments shown above and/or to be accorded the widest scope consistent with the principles and novel features disclosed in any fashion herein.

Claims (21)

1. A composition for oral administration comprising:

supercritical and hydroalcoholic turmeric extracts,

wherein the oral administration composition comprises supercritical extract and hydroalcoholic extract of Curcuma longa in an amount effective for detoxifying human smoke.

2. The composition of claim 1, wherein the weight ratio of the hydroalcoholic extract to the supercritical extract comprises a weight ratio of about 3.0-6.0 parts hydroalcoholic extract to 1 part supercritical extract.

3. The composition of claim 1, wherein the composition comprises at least one of curcuminoids, antioxidants, and turmerone.

4. The composition as set forth in claim 1, wherein,

wherein said supercritical extract of turmeric comprises about 43% to 47% turmerones; and is

Wherein said hydroalcoholic turmeric extract contains about 11% to about 15% curcuminoids.

5. The composition of claim 1, further comprising:

an aqueous extract of green tea leaves,

wherein at least one of the supercritical extract and hydroalcoholic extract of turmeric acts synergistically with the aqueous extract of green tea.

6. A composition for oral administration comprising:

supercritical and hydroalcoholic extracts of turmeric and ginger;

supercritical extracts of rosemary, parsley seed, peppermint and clove;

hydroalcoholic extracts of rosemary, parsley leaf, peppermint and clove; and

an aqueous extract of green tea leaves,

wherein the oral administration composition consists of the following components with effective dose for realizing human smoke toxicity removal: (i) supercritical and hydroalcoholic extracts of turmeric and ginger; (ii) supercritical extracts of rosemary, parsley seed, peppermint and clove; and (iii) hydroalcoholic extracts of rosemary, parsley leaf, peppermint, and clove.

7. The composition of claim 6, wherein the weight ratio of the hydroalcoholic turmeric extract to the supercritical turmeric extract comprises a weight ratio of about 3.0-6.0 parts hydroalcoholic extract to 1 part supercritical extract.

8. The composition of claim 6, wherein the weight ratio of the hydroalcoholic ginger extract to the supercritical ginger extract comprises a weight ratio of about 4.4 parts hydroalcoholic extract to 1 part supercritical extract.

9. The composition of claim 6, wherein the composition comprises the following components in the following weight ratios: (i) (ii) the hydroalcoholic rosemary extract to the supercritical rosemary extract from about 1.75: 1 to about 2.25: 1; (ii) (ii) a hydroalcoholic parsley leaf extract to a supercritical parsley seed extract from about 1.75: 1 to about 2.25: 1; (iii) (ii) a hydroalcoholic ginger extract to a supercritical ginger extract from about 4: 1 to about 6: 1; (iv) (ii) a hydroalcoholic peppermint extract to a supercritical peppermint extract from about 1.75: 1 to about 2.25: 1; (v) (ii) a hydroalcoholic clove extract to supercritical clove extract from about 1.75: 1 to about 2.25: 1; and (vi) about 1: 1 to 2: 1 of extracts of turmeric, rosemary, parsley, ginger, peppermint and clove and green tea extract.

10. The composition of claim 6, wherein the composition comprises the following components by weight: (i) about 45% -55% of hydroalcoholic and supercritical turmeric extracts; (ii) from about 1.5% to 2.5% of the supercritical and hydroalcoholic rosemary extract; (iii) from about 1.5% to about 2.5% by weight of the supercritical parsley seed extract and hydroalcoholic parsley leaf extract; (iv) about 1.5% to 2.5% of the hydroalcoholic and supercritical ginger extracts; (v) from about 1.5% to about 2.5% of supercritical and hydroalcoholic peppermint extracts; (vi) about 1.5% -2.5% supercritical and hydroalcoholic clove extracts; and (vii) about 35% to 45% of an aqueous green tea extract.

11. A method of effecting smoke detoxification in a human comprising forming a composition comprised of an effective amount of a supercritical extract and a hydroalcoholic extract of turmeric, and orally administering the composition to the human in order to reduce the levels of mutagens and fibrinogen in the human exposed to smoke detoxification.

12. The method of claim 11, wherein the forming a composition comprises forming a composition having a weight ratio of hydroalcoholic turmeric extract to supercritical turmeric extract comprising a weight ratio of about 3.0-6.0 parts hydroalcoholic extract to 1 part supercritical extract.

13. The method of claim 11, wherein the forming comprises forming a composition having at least one of curcuminoids, antioxidants, and turmerone.

14. The method of claim 11, wherein the step of treating the sample with the acid is carried out in a single step,

the supercritical extract of turmeric contains about 43% to 47% of turmerones; and is

Wherein said hydroalcoholic turmeric extract contains about 11% to about 15% curcuminoids.

15. The method of claim 11, wherein said forming a composition comprises adding an effective amount of an aqueous extract of green tea.

16. The method of claim 11, wherein said oral administration comprises orally administering the composition to a human exposed to a smoke toxin for a therapeutically effective period of time.

17. A method of effecting smoke detoxification in a human comprising forming a composition consisting of effective amounts of: (i) supercritical and hydroalcoholic extracts of turmeric and ginger; (ii) supercritical extracts of rosemary, parsley seed, peppermint and clove; and (iii) hydroalcoholic extracts of rosemary, parsley leaf, peppermint, and clove; and orally administering the composition to a person in contact with the tobacco toxicant.

18. The method of claim 17, wherein the forming comprises forming a composition having a weight ratio of hydroalcoholic turmeric extract to supercritical turmeric extract of about 3.0-6.0 parts hydroalcoholic extract to 1 part supercritical extract.

19. The method of claim 17, wherein the forming comprises forming the composition with a weight ratio of hydroalcoholic ginger extract to supercritical ginger extract of about 4.4 parts hydroalcoholic extract to 1 part supercritical extract.

20. The method of claim 17, wherein the forming comprises forming a composition comprising the following components in the following weight ratios: (i) (ii) the hydroalcoholic rosemary extract to the supercritical rosemary extract from about 1.75: 1 to about 2.25: 1; (ii) (ii) a hydroalcoholic parsley leaf extract to a supercritical parsley seed extract from about 1.75: 1 to about 2.25: 1; (iii) (ii) a hydroalcoholic ginger extract to a supercritical ginger extract from about 4: 1 to about 6: 1; (iv) (ii) a hydroalcoholic peppermint extract to a supercritical peppermint extract from about 1.75: 1 to about 2.25: 1; (v) (ii) a hydroalcoholic clove extract to supercritical clove extract from about 1.75: 1 to about 2.25: 1; and (vi) about 1: 1 to 2: 1 of extracts of turmeric, rosemary, parsley, ginger, peppermint and clove and green tea extract.

21. The method of claim 17, wherein the forming comprises forming a composition comprising the following components by weight: (i) about 45% -55% of hydroalcoholic and supercritical turmeric extracts; (ii) from about 1.5% to 2.5% of the supercritical and hydroalcoholic rosemary extract; (iii) from about 1.5% to about 2.5% by weight of the supercritical parsley seed extract and hydroalcoholic parsley leaf extract; (iv) about 1.5% to 2.5% of the hydroalcoholic and supercritical ginger extracts; (v) from about 1.5% to about 2.5% of supercritical and hydroalcoholic peppermint extracts; (vi) from about 1.5% to about 2.5% of the supercritical and hydroalcoholic clove extracts; and (vii) about 35% to 45% of an aqueous green tea extract.