GR1008010B - Dish for differential synchronous cultures - Google Patents
Dish for differential synchronous culturesInfo
- Publication number
- GR1008010B GR1008010B GR20120100431A GR20120100431A GR1008010B GR 1008010 B GR1008010 B GR 1008010B GR 20120100431 A GR20120100431 A GR 20120100431A GR 20120100431 A GR20120100431 A GR 20120100431A GR 1008010 B GR1008010 B GR 1008010B
- Authority
- GR
- Greece
- Prior art keywords
- dish
- growth
- sabouraud
- mycological
- color
- Prior art date
Links
- 230000001360 synchronised effect Effects 0.000 title 1
- 235000015097 nutrients Nutrition 0.000 abstract 3
- 241000233866 Fungi Species 0.000 abstract 2
- 238000004458 analytical method Methods 0.000 abstract 2
- 239000000463 material Substances 0.000 abstract 2
- 239000002184 metal Substances 0.000 abstract 2
- 238000005192 partition Methods 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 229920001817 Agar Polymers 0.000 abstract 1
- 239000008272 agar Substances 0.000 abstract 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 abstract 1
- 229960005091 chloramphenicol Drugs 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000012449 sabouraud dextrose agar Substances 0.000 abstract 1
- 238000012421 spiking Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
- C12Q1/08—Quantitative determination using multifield media
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Plastic dishes having a diameter of 9 cm, radially divided in three sectors, each of 120 degrees, are filled with three different commercially available nutrient substrates for mycological use: MEA, CzA and Sabouraud Glycose Agar or Sabouraud Dextrose Agar or Sabouraud Emmon's, with or without chloramphenicol. These nutrient substrates may be replaced by others, depending on the expected fungi populations, however one of these must be a general common one for mycological use, a second one must induce intense growth and the third one should be oligotrophic, selective or differentiating. The dish, as fabricated, has on one partition wall a visible color marking or a metal insert for fixed orientation of the dish during filling clockwise with the three materials, in the said order or in any other fixed order. Each dish is inoculated at the three materials by spiking of the same fungus sample strain and incubated. When analysing the dish, the growth of the strain on the three nutrients under almost identical growth conditions (temperature, humidity, inoculation time) is compared. The label (color or metal) on a partition allows the automatization of the incubation procedure, while repeated analyses over time of the same dish allow plotting of growth curves instead of simple, momentary descriptions. Analysis of the dishes, either by an image processing software or by naked eye, and filling of a description report may be facilitated by scanning the dish with a color scanner.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GR20120100431A GR1008010B (en) | 2012-08-24 | 2012-08-24 | Dish for differential synchronous cultures |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GR20120100431A GR1008010B (en) | 2012-08-24 | 2012-08-24 | Dish for differential synchronous cultures |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GR1008010B true GR1008010B (en) | 2013-10-16 |
Family
ID=47681965
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GR20120100431A GR1008010B (en) | 2012-08-24 | 2012-08-24 | Dish for differential synchronous cultures |
Country Status (1)
| Country | Link |
|---|---|
| GR (1) | GR1008010B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3055808A (en) * | 1959-07-23 | 1962-09-25 | Baltimore Biolog Lab Inc | Hermetically sealed petri dish |
| FR2257210A6 (en) * | 1973-12-18 | 1975-08-01 | Tournade Francois | Microbial sampling appts. for suspended particles in air - using nutrient media to trap bacteria for analysis |
| US3960658A (en) * | 1974-09-23 | 1976-06-01 | Centaur Chemical Co. | Multi-media petri dish |
| WO2009026920A1 (en) * | 2007-08-31 | 2009-03-05 | Statens Serum Institut | Compositions and means for diagnosing microbial infections |
-
2012
- 2012-08-24 GR GR20120100431A patent/GR1008010B/en not_active IP Right Cessation
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3055808A (en) * | 1959-07-23 | 1962-09-25 | Baltimore Biolog Lab Inc | Hermetically sealed petri dish |
| FR2257210A6 (en) * | 1973-12-18 | 1975-08-01 | Tournade Francois | Microbial sampling appts. for suspended particles in air - using nutrient media to trap bacteria for analysis |
| US3960658A (en) * | 1974-09-23 | 1976-06-01 | Centaur Chemical Co. | Multi-media petri dish |
| WO2009026920A1 (en) * | 2007-08-31 | 2009-03-05 | Statens Serum Institut | Compositions and means for diagnosing microbial infections |
Non-Patent Citations (1)
| Title |
|---|
| REEDER J C ET AL: "COMPARISON OF THREE-COMPARTMENT PETRI DISHES AND INDIVIDUAL PLATES FOR ROUTINE CULTURE OF VAGINAL SWABS", 1990, JOURNAL OF CLINICAL PATHOLOGY (LONDON), VOL. 43, NR. 11, PAGE(S) 947-949, ISSN: 0021-9746, XP002699937 * |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PG | Patent granted |
Effective date: 20131118 |
|
| ML | Lapse due to non-payment of fees |
Effective date: 20160303 |