GB938163A - Improvements in or relating to particle size reduction or cellular disruption - Google Patents
Improvements in or relating to particle size reduction or cellular disruptionInfo
- Publication number
- GB938163A GB938163A GB3229960A GB3229960A GB938163A GB 938163 A GB938163 A GB 938163A GB 3229960 A GB3229960 A GB 3229960A GB 3229960 A GB3229960 A GB 3229960A GB 938163 A GB938163 A GB 938163A
- Authority
- GB
- United Kingdom
- Prior art keywords
- inert bodies
- liquid
- inert
- bodies
- transducer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 230000001413 cellular effect Effects 0.000 title abstract 2
- 238000010951 particle size reduction Methods 0.000 title 1
- 239000007788 liquid Substances 0.000 abstract 10
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 abstract 6
- 239000000725 suspension Substances 0.000 abstract 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract 4
- 239000000463 material Substances 0.000 abstract 3
- 239000003883 ointment base Substances 0.000 abstract 3
- 239000011324 bead Substances 0.000 abstract 2
- 239000011521 glass Substances 0.000 abstract 2
- 235000019271 petrolatum Nutrition 0.000 abstract 2
- 239000000523 sample Substances 0.000 abstract 2
- 229910001220 stainless steel Inorganic materials 0.000 abstract 2
- 239000010935 stainless steel Substances 0.000 abstract 2
- 239000004094 surface-active agent Substances 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- KZDCMKVLEYCGQX-UDPGNSCCSA-N 2-(diethylamino)ethyl 4-aminobenzoate;(2s,5r,6r)-3,3-dimethyl-7-oxo-6-[(2-phenylacetyl)amino]-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid;hydrate Chemical compound O.CCN(CC)CCOC(=O)C1=CC=C(N)C=C1.N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 KZDCMKVLEYCGQX-UDPGNSCCSA-N 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 241000191967 Staphylococcus aureus Species 0.000 abstract 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 abstract 1
- 239000005864 Sulphur Substances 0.000 abstract 1
- 239000010419 fine particle Substances 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
- 239000011236 particulate material Substances 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
- 150000003431 steroids Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
- C12N1/066—Lysis of microorganisms by physical methods
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C17/00—Disintegrating by tumbling mills, i.e. mills having a container charged with the material to be disintegrated with or without special disintegrating members such as pebbles or balls
- B02C17/14—Mills in which the charge to be ground is turned over by movements of the container other than by rotating, e.g. by swinging, vibrating, tilting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C19/00—Other disintegrating devices or methods
- B02C19/18—Use of auxiliary physical effects, e.g. ultrasonics, irradiation, for disintegrating
Landscapes
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
A process for reducing the particle size of a particulate material comprises subjecting to ultrasonic vibrations a suspension of the material in a chemically inert liquid in the presence of small chemically inert bodies. The suspension may be agitated by means of a hollow stirrer. The inert bodies may be glass beads or stainless steel balls. The inert liquid, which may contain a surface active agent, may be water, a volatile substantially anhydrous liquid e.g. benzene or ether or a solution of an ointment base e.g. petroleum jelly, in a volatile liquid. Apparatus for carrying out the process comprises a combination of an ultrasonic transducer and a vessel containing small inert bodies, the combination being arranged such that the inert bodies may be subjected to ultrasonic vibrations. The transducer may be attached to the base of a vessel containing the inert bodies. In an alternative, an inner vessel may contain the inert bodies and an outer vessel have the transducer attached thereto, the space between the vessels containing a liquid. In a further alternative, a probe with attached ultrasonic transducer may be inserted in a vessel containing the inert bodies. The apparatus may have inlet and outlet means so that it may be used on a continuous basis. Examples are described.ALSO:A process for disrupting cellular material comprises subjecting to ultrasonic vibrations a suspension of the material in a chemically inert liquid in the presence of small chemically inert bodies. The suspension may be agitated by means of a hollow stirrer. The inert bodies may be glass beads or stainless steel balls. The inert liquid, which may contain a surface-active agent, may be water, a volatile substantially anhydrous liquid, e.g. benzene or ether or a solution of an ointment base e.g. petroleum jelly, in a volatile liquid in which case after the process has been completed, the volatile solvent is easily removed to leave a highly dispersed suspension of the fine particles in the ointment base. Apparatus for carrying out the process comprises a combination of an ultrasonic transducer and a vessel containing small inert bodies, the combination being arranged such that the inert bodies may be subjected to ultrasonic vibrations. The transducer may be attached to the base of a vessel containing the inert bodies. In an alternative, an inner vessel may contain the inert bodies and an outer vessel have the transducer attached thereto, the space between the vessels containing a liquid. In a further alternative, a probe with attached ultrasonic transducer may be inserted in a vessel containing the inert bodies. The apparatus may have inlet and outlet means so that it may be used on a continuous basis. Examples are described of the subdivision of procaine penicillin, a steroid, staphylococcus aureus, yeast and sulphur.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB3229960A GB938163A (en) | 1960-09-20 | 1960-09-20 | Improvements in or relating to particle size reduction or cellular disruption |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB3229960A GB938163A (en) | 1960-09-20 | 1960-09-20 | Improvements in or relating to particle size reduction or cellular disruption |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB938163A true GB938163A (en) | 1963-10-02 |
Family
ID=10336446
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB3229960A Expired GB938163A (en) | 1960-09-20 | 1960-09-20 | Improvements in or relating to particle size reduction or cellular disruption |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB938163A (en) |
Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62236478A (en) * | 1986-03-20 | 1987-10-16 | ジエン−プロ−ブ・インコ−ポレイテツド | Separation of rna and dna from cell |
| FR2618793A1 (en) * | 1987-07-10 | 1989-02-03 | Berlin Kosmetik Veb | METHOD AND DEVICE FOR OBTAINING BIO-ACTIVE SUSPENSIONS |
| EP0796917A3 (en) * | 1996-03-12 | 1998-11-25 | Becton, Dickinson and Company | Vehicle for delivery of particles to a sample |
| WO1999058637A3 (en) * | 1998-05-07 | 2000-01-27 | Fraunhofer Ges Forschung | Device and method for targeted exposure of a biological sample to sound waves |
| US6071480A (en) * | 1994-12-22 | 2000-06-06 | Abbott Laboratories | Method for generating a standing sonic wave, methods of sonication with a standing sonic wave, and a standing sonic wave sonicator |
| FR2791697A1 (en) * | 1999-04-01 | 2000-10-06 | Biomerieux Sa | Apparatus for ultrasonic cell lysis, useful for recovering nucleic acids from bacteria and yeast, in which the probe has an active surface that matches the shape of the sample vessel |
| WO2000073413A3 (en) * | 1999-05-28 | 2001-01-25 | Cepheid | Apparatus and method for cell disruption |
| EP0897989A3 (en) * | 1997-08-15 | 2002-06-26 | Becton, Dickinson and Company | Vehicle for delivery of particles to a sample |
| US6431476B1 (en) | 1999-12-21 | 2002-08-13 | Cepheid | Apparatus and method for rapid ultrasonic disruption of cells or viruses |
| US6440725B1 (en) | 1997-12-24 | 2002-08-27 | Cepheid | Integrated fluid manipulation cartridge |
| US6664104B2 (en) | 1999-06-25 | 2003-12-16 | Cepheid | Device incorporating a microfluidic chip for separating analyte from a sample |
| US6818185B1 (en) | 1999-05-28 | 2004-11-16 | Cepheid | Cartridge for conducting a chemical reaction |
| WO2009149298A1 (en) | 2008-06-05 | 2009-12-10 | Sonoash Llc | Upgraded combustion ash and its method of production |
| US7914994B2 (en) | 1998-12-24 | 2011-03-29 | Cepheid | Method for separating an analyte from a sample |
| US8268603B2 (en) | 1999-05-28 | 2012-09-18 | Cepheid | Apparatus and method for cell disruption |
| US8441629B2 (en) | 2009-04-15 | 2013-05-14 | Biocartis Sa | Optical detection system for monitoring rtPCR reaction |
| US8512637B2 (en) | 2009-05-06 | 2013-08-20 | Biocartis Sa | Device for cutting a sample carrier |
| US8641971B2 (en) | 2009-04-14 | 2014-02-04 | Biocartis Sa | HIFU induced cavitation with reduced power threshold |
| US8815521B2 (en) | 2000-05-30 | 2014-08-26 | Cepheid | Apparatus and method for cell disruption |
| US9073053B2 (en) | 1999-05-28 | 2015-07-07 | Cepheid | Apparatus and method for cell disruption |
| US9079182B2 (en) | 2009-04-15 | 2015-07-14 | Biocartis Nv | Protection of bioanalytical sample chambers |
-
1960
- 1960-09-20 GB GB3229960A patent/GB938163A/en not_active Expired
Cited By (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0288618A1 (en) * | 1986-03-20 | 1988-11-02 | Gen-Probe Incorporated | Method for releasing RNA and DNA from cells |
| JPH0813272B2 (en) | 1986-03-20 | 1996-02-14 | ジエン−プローブ・インコーポレイテツド | Method for releasing RNA and DNA from cells |
| JPS62236478A (en) * | 1986-03-20 | 1987-10-16 | ジエン−プロ−ブ・インコ−ポレイテツド | Separation of rna and dna from cell |
| FR2618793A1 (en) * | 1987-07-10 | 1989-02-03 | Berlin Kosmetik Veb | METHOD AND DEVICE FOR OBTAINING BIO-ACTIVE SUSPENSIONS |
| US6071480A (en) * | 1994-12-22 | 2000-06-06 | Abbott Laboratories | Method for generating a standing sonic wave, methods of sonication with a standing sonic wave, and a standing sonic wave sonicator |
| EP0796917A3 (en) * | 1996-03-12 | 1998-11-25 | Becton, Dickinson and Company | Vehicle for delivery of particles to a sample |
| US6893879B2 (en) | 1997-08-13 | 2005-05-17 | Cepheid | Method for separating analyte from a sample |
| EP0897989A3 (en) * | 1997-08-15 | 2002-06-26 | Becton, Dickinson and Company | Vehicle for delivery of particles to a sample |
| US6440725B1 (en) | 1997-12-24 | 2002-08-27 | Cepheid | Integrated fluid manipulation cartridge |
| US7569346B2 (en) | 1997-12-24 | 2009-08-04 | Cepheid | Method for separating analyte from a sample |
| WO1999058637A3 (en) * | 1998-05-07 | 2000-01-27 | Fraunhofer Ges Forschung | Device and method for targeted exposure of a biological sample to sound waves |
| US6699711B1 (en) * | 1998-05-07 | 2004-03-02 | Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. | Device and method for selective exposure of a biological sample to sound waves |
| US8247176B2 (en) | 1998-12-24 | 2012-08-21 | Cepheid | Method for separating an analyte from a sample |
| US7914994B2 (en) | 1998-12-24 | 2011-03-29 | Cepheid | Method for separating an analyte from a sample |
| US8592157B2 (en) | 1998-12-24 | 2013-11-26 | Cepheid | Method for separating an analyte from a sample |
| US6987018B2 (en) | 1998-12-24 | 2006-01-17 | Cepheid | Container for holding cells or viruses for disruption |
| US6887693B2 (en) | 1998-12-24 | 2005-05-03 | Cepheid | Device and method for lysing cells, spores, or microorganisms |
| US6686195B1 (en) | 1999-04-01 | 2004-02-03 | Biomerieux S.A. | Method and apparatus for ultrasonic lysis of biological cells |
| WO2000060049A1 (en) * | 1999-04-01 | 2000-10-12 | Biomerieux S.A. | Method and apparatus for ultrasonic lysis of biological cells |
| FR2791697A1 (en) * | 1999-04-01 | 2000-10-06 | Biomerieux Sa | Apparatus for ultrasonic cell lysis, useful for recovering nucleic acids from bacteria and yeast, in which the probe has an active surface that matches the shape of the sample vessel |
| EP1466966A1 (en) * | 1999-04-01 | 2004-10-13 | Biomerieux S.A. | Apparatus and process of lysis by biological ultrasounds of cells |
| US6881541B2 (en) | 1999-05-28 | 2005-04-19 | Cepheid | Method for analyzing a fluid sample |
| US8580559B2 (en) | 1999-05-28 | 2013-11-12 | Cepheid | Device for extracting nucleic acid from a sample |
| US6818185B1 (en) | 1999-05-28 | 2004-11-16 | Cepheid | Cartridge for conducting a chemical reaction |
| US6783736B1 (en) | 1999-05-28 | 2004-08-31 | Cepheid | Cartridge for analyzing a fluid sample |
| US9943848B2 (en) | 1999-05-28 | 2018-04-17 | Cepheid | Apparatus and method for cell disruption |
| US9789481B2 (en) | 1999-05-28 | 2017-10-17 | Cepheid | Device for extracting nucleic acid from a sample |
| US9322052B2 (en) | 1999-05-28 | 2016-04-26 | Cepheid | Cartridge for conducting a chemical reaction |
| US6391541B1 (en) | 1999-05-28 | 2002-05-21 | Kurt E. Petersen | Apparatus for analyzing a fluid sample |
| US8168442B2 (en) | 1999-05-28 | 2012-05-01 | Cepheid | Cartridge for conducting a chemical reaction |
| WO2000073412A3 (en) * | 1999-05-28 | 2002-03-21 | Cepheid | Apparatus and method for analyzing a fluid sample |
| US8268603B2 (en) | 1999-05-28 | 2012-09-18 | Cepheid | Apparatus and method for cell disruption |
| US9073053B2 (en) | 1999-05-28 | 2015-07-07 | Cepheid | Apparatus and method for cell disruption |
| US8709363B2 (en) | 1999-05-28 | 2014-04-29 | Cepheid | Cartridge for conducting a chemical reaction |
| WO2000073413A3 (en) * | 1999-05-28 | 2001-01-25 | Cepheid | Apparatus and method for cell disruption |
| US6878540B2 (en) | 1999-06-25 | 2005-04-12 | Cepheid | Device for lysing cells, spores, or microorganisms |
| US6664104B2 (en) | 1999-06-25 | 2003-12-16 | Cepheid | Device incorporating a microfluidic chip for separating analyte from a sample |
| US6431476B1 (en) | 1999-12-21 | 2002-08-13 | Cepheid | Apparatus and method for rapid ultrasonic disruption of cells or viruses |
| US8815521B2 (en) | 2000-05-30 | 2014-08-26 | Cepheid | Apparatus and method for cell disruption |
| WO2009149298A1 (en) | 2008-06-05 | 2009-12-10 | Sonoash Llc | Upgraded combustion ash and its method of production |
| EP2303461A4 (en) * | 2008-06-05 | 2017-04-19 | Provectus Engineered Materiels Ltd. | Upgraded combustion ash and its method of production |
| US8641971B2 (en) | 2009-04-14 | 2014-02-04 | Biocartis Sa | HIFU induced cavitation with reduced power threshold |
| US8986612B2 (en) | 2009-04-14 | 2015-03-24 | Biocartis Nv | HIFU induced cavitation with reduced power threshold |
| US9097626B2 (en) | 2009-04-14 | 2015-08-04 | Biocartis Nv | HIFU induced cavitation with reduced power threshold |
| US8441629B2 (en) | 2009-04-15 | 2013-05-14 | Biocartis Sa | Optical detection system for monitoring rtPCR reaction |
| US9079182B2 (en) | 2009-04-15 | 2015-07-14 | Biocartis Nv | Protection of bioanalytical sample chambers |
| US8512637B2 (en) | 2009-05-06 | 2013-08-20 | Biocartis Sa | Device for cutting a sample carrier |
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