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GB866488A - A process for the manufacture of vitamins of b-group - Google Patents

A process for the manufacture of vitamins of b-group

Info

Publication number
GB866488A
GB866488A GB42212/59A GB4221259A GB866488A GB 866488 A GB866488 A GB 866488A GB 42212/59 A GB42212/59 A GB 42212/59A GB 4221259 A GB4221259 A GB 4221259A GB 866488 A GB866488 A GB 866488A
Authority
GB
United Kingdom
Prior art keywords
casein
phosphate
acid
biotin
vitamin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB42212/59A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of GB866488A publication Critical patent/GB866488A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H23/00—Compounds containing boron, silicon or a metal, e.g. chelates or vitamin B12
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/42—Cobalamins, i.e. vitamin B12, LLD factor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Vitamin B12 is obtained by fermenting a medium containing carbohydrates, amino acids and nutrient salts using Propionibacterium shermanii Strain 33 as the organism. The process is preferably carried out under anaerobic conditions at 28 DEG to 32 DEG C. at a pH of 6 to 7 with a sugar concentration of 1-2% by weight and for a period of 10-12 days. The fermentation medium is preferably obtained by mixing a fermentable sugar such as glucose, invert sugar or maltose, with an amino-acid mixture obtained by degrading wheat- or corn-gluten, potato- or soya-protein, casein, blood-, or fish albumin, and with pantothenic acid, biotin, sources of cobalt, sodium, potassium, magnesium, iron, phosphate, sulphate and chloride ions-, yeast extract and 5.6-dimethylbenzimidazole in alcoholic solution. Vitamin B12 may be separated by adjusting the pH to 6, heating to 85-120 DEG C., centrifiuging, adsorbing on active carbon, eluting with an aqueous alcohol such as isopropanol, and then crystallising. P shermanii Strain 33 is obtained by growing milk curd on a medium containing glucose, casein proteolyzate, casein acid hydrolysate, sodium dihydrogen phosphate, tripotassium phosphate, magnesium chloride hexahydrate, ferrous sulphate heptahydrate, cobalt sulphate heptahydrate, pantothenic acid, biotin and yeast extract, and selectively isolating cells of the diplo form which occur in short chains having at most 4 members.
GB42212/59A 1958-12-24 1959-12-11 A process for the manufacture of vitamins of b-group Expired GB866488A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CH866488X 1958-12-24

Publications (1)

Publication Number Publication Date
GB866488A true GB866488A (en) 1961-04-26

Family

ID=4543688

Family Applications (1)

Application Number Title Priority Date Filing Date
GB42212/59A Expired GB866488A (en) 1958-12-24 1959-12-11 A process for the manufacture of vitamins of b-group

Country Status (1)

Country Link
GB (1) GB866488A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1022244B (en) * 1956-04-21 1958-01-09 Lentia Gmbh Fertilizers or soil improvers containing micronutrients and processes for their production
WO1996033283A1 (en) * 1995-04-21 1996-10-24 Takeda Chemical Industries, Ltd. Process for producing calcium d-pantothenate
ES2102332A1 (en) * 1996-01-03 1997-07-16 Invest Y Desarrollo Agroindust Process for the production of vitamin B12 from polluting wastes in the citrus growing industries.

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1022244B (en) * 1956-04-21 1958-01-09 Lentia Gmbh Fertilizers or soil improvers containing micronutrients and processes for their production
WO1996033283A1 (en) * 1995-04-21 1996-10-24 Takeda Chemical Industries, Ltd. Process for producing calcium d-pantothenate
US6013492A (en) * 1995-04-21 2000-01-11 Takeda Chemical Industries, Ltd. Microbial process for producing calcium D-pantothenate
ES2102332A1 (en) * 1996-01-03 1997-07-16 Invest Y Desarrollo Agroindust Process for the production of vitamin B12 from polluting wastes in the citrus growing industries.

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