GB854918A - Azotobacter concentrates obtained by submerged aerobic fermentation - Google Patents
Azotobacter concentrates obtained by submerged aerobic fermentationInfo
- Publication number
- GB854918A GB854918A GB3066/57A GB306657A GB854918A GB 854918 A GB854918 A GB 854918A GB 3066/57 A GB3066/57 A GB 3066/57A GB 306657 A GB306657 A GB 306657A GB 854918 A GB854918 A GB 854918A
- Authority
- GB
- United Kingdom
- Prior art keywords
- culture
- sterile
- waste
- culture medium
- magnesium carbonate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 241000589151 Azotobacter Species 0.000 title abstract 5
- 239000012141 concentrate Substances 0.000 title abstract 3
- 238000010564 aerobic fermentation Methods 0.000 title 1
- 239000002699 waste material Substances 0.000 abstract 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 8
- 239000001963 growth medium Substances 0.000 abstract 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 6
- 239000003463 adsorbent Substances 0.000 abstract 6
- 239000008103 glucose Substances 0.000 abstract 6
- ZLNQQNXFFQJAID-UHFFFAOYSA-L magnesium carbonate Chemical compound [Mg+2].[O-]C([O-])=O ZLNQQNXFFQJAID-UHFFFAOYSA-L 0.000 abstract 6
- 239000001095 magnesium carbonate Substances 0.000 abstract 6
- 229910000021 magnesium carbonate Inorganic materials 0.000 abstract 6
- 239000002689 soil Substances 0.000 abstract 6
- 239000005995 Aluminium silicate Substances 0.000 abstract 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 abstract 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 4
- 229920002472 Starch Polymers 0.000 abstract 4
- 238000005273 aeration Methods 0.000 abstract 4
- 235000012211 aluminium silicate Nutrition 0.000 abstract 4
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 abstract 4
- 229910052757 nitrogen Inorganic materials 0.000 abstract 4
- 238000002360 preparation method Methods 0.000 abstract 4
- 239000008107 starch Substances 0.000 abstract 4
- 235000019698 starch Nutrition 0.000 abstract 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 4
- 229920001353 Dextrin Polymers 0.000 abstract 2
- 239000004375 Dextrin Substances 0.000 abstract 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 2
- 229910052925 anhydrite Inorganic materials 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 abstract 2
- 210000000988 bone and bone Anatomy 0.000 abstract 2
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 abstract 2
- 229910052799 carbon Inorganic materials 0.000 abstract 2
- JYYOBHFYCIDXHH-UHFFFAOYSA-N carbonic acid;hydrate Chemical compound O.OC(O)=O JYYOBHFYCIDXHH-UHFFFAOYSA-N 0.000 abstract 2
- 235000008504 concentrate Nutrition 0.000 abstract 2
- 235000019425 dextrin Nutrition 0.000 abstract 2
- 238000007865 diluting Methods 0.000 abstract 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 abstract 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 abstract 2
- 235000019797 dipotassium phosphate Nutrition 0.000 abstract 2
- 239000000428 dust Substances 0.000 abstract 2
- 238000000855 fermentation Methods 0.000 abstract 2
- 230000004151 fermentation Effects 0.000 abstract 2
- 239000003864 humus Substances 0.000 abstract 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 abstract 2
- 235000019341 magnesium sulphate Nutrition 0.000 abstract 2
- 239000000463 material Substances 0.000 abstract 2
- 238000002156 mixing Methods 0.000 abstract 2
- 235000015097 nutrients Nutrition 0.000 abstract 2
- 239000000741 silica gel Substances 0.000 abstract 2
- 229910002027 silica gel Inorganic materials 0.000 abstract 2
- 239000011780 sodium chloride Substances 0.000 abstract 2
- 235000002639 sodium chloride Nutrition 0.000 abstract 2
- 239000011684 sodium molybdate Substances 0.000 abstract 2
- 235000015393 sodium molybdate Nutrition 0.000 abstract 2
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- 239000008223 sterile water Substances 0.000 abstract 2
- 238000003756 stirring Methods 0.000 abstract 2
- 235000020357 syrup Nutrition 0.000 abstract 2
- 239000006188 syrup Substances 0.000 abstract 2
- 238000005406 washing Methods 0.000 abstract 2
- 239000002351 wastewater Substances 0.000 abstract 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/065—Azotobacter
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Fodder In General (AREA)
Abstract
A concentrated azotobacter culture for use in soil bacterizing and fodder preparations is obtained by preparing a sterile culture medium from nutrients and assimilable carbonaceous waste material free or substantially free of nitrogen, diluting same with sterile water, adding thereto sterile magnesium carbonate and a sterile adsorbent, stirring, inoculating with an azotobacter culture with simultaneous aeration, fermenting until substantially all carbonaceous waste material has been utilised and thereafter adding a further quantity of said culture medium, magnesium carbonate and adsorbent, allowing fermentation to proceed at a pH value of 6,5 to 7,0 with aeration until substantially all the carbon source has been utilised and separating, washing and rethickening the mature mash twice to obtain a bacterial concentrate. In the sample, the culture medium contains glucose waste (74,4% glucose, 18,5% dextrin and 0,032% nitrogen in a solids content of 94,8%) KHPO4, K2HPO4, MgSO4, NaCl, CaSO4, sodium molybdate and water and magnesium carbonate and activated carbon is added. In place of the glucose waste, there may be used waste starch, starch refuse, syrups and waste waters containing such materials. Other adsorbents are bone dust, kaolin, Fuller's earth and silica gel. The temperature of cultivation is 25 to 30 DEG C. and the duration 36 to 48 hours. The final culture may be used to inoculate a fresh batch of culture medium. A soil bacterizing preparation is produced by mixing the final culture with sterile humus soil, kaolin or fuller's earth in proportions of 1 to 20 to 1 to 100. In the case of an animal feed, the final culture is mixed with animal fodder.ALSO:A concentrated azotobacter culture for use in soil bacterizing and fodder preparations is obtained by preparing a sterile culture medium from nutrients and assimilable carbonaceous waste material free or substantially free of nitrogen, diluting same with sterile water, adding thereto sterile magnesium carbonate and a sterile adsorbent, stirring, inoculating with an azotobacter culture with simultaneous aeration, fermenting until substantially all carbonaceous waste material has been utilised and thereafter adding a further quantity of said culture medium, magnesium carbonate and adsorbent, allowing fermentation to proceed at a pH value of 6,5 to 7,0 with aeration until substantially all the carbon source has been utilized and separating, washing and re-thickening the mature mash twice to obtain a bacterial concentrate. In the example, the culture medium contains glucose waste (74,4% glucose, 18,5% dextrin and 0,032% nitrogen in a solids content of 94,8%) KHPO4, K2HPO4, MgSO4, NaCl, CaSO4, sodium molybdate and water and magnesium carbonate and activated carbon is added. In place of the glucose waste, there may be used waste starch, starch refuse, syrups and waste waters containing such materials. Other adsorbents are bone dust, kaolin, Fuller's earth and silica gel. The temperature of cultivation is 25 to 30 DEG C. and the duration 36 to 48 hours. The final culture may be used to inoculate a fresh batch of culture medium. A soil bacterizing preparation is produced by mixing the final culture with sterile humus soil, kaolin, or Fuller's earth in proportions of 1 to 20 to 1 to 100. In the case of an animal feed, the final culture is mixed with animal fodder.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS854918X | 1956-02-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB854918A true GB854918A (en) | 1960-11-23 |
Family
ID=5456188
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB3066/57A Expired GB854918A (en) | 1956-02-06 | 1957-01-29 | Azotobacter concentrates obtained by submerged aerobic fermentation |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB854918A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3205060A (en) * | 1962-03-26 | 1965-09-07 | Standard Oil Co | Soil conditioning composition |
| FR2352055A1 (en) * | 1976-05-18 | 1977-12-16 | Mueller Hans | MICROORGANISMS RECYCLING PROCESS |
| EP0341736A1 (en) * | 1988-05-13 | 1989-11-15 | BIOCHEMIE Gesellschaft m.b.H. | Fertilizer comprising bacterial biomass, process for its production and its use as a special organic fertilizer |
| EP0369223A1 (en) * | 1988-10-27 | 1990-05-23 | Biochemie Gesellschaft M.B.H. | Bacterial biomass fertilizer |
| WO2018096429A1 (en) * | 2016-11-22 | 2018-05-31 | Solyom Zoltan | Procedure for preserving soil bacteria, suitably to produce preserved soil-improving bio-fertilizers |
-
1957
- 1957-01-29 GB GB3066/57A patent/GB854918A/en not_active Expired
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3205060A (en) * | 1962-03-26 | 1965-09-07 | Standard Oil Co | Soil conditioning composition |
| FR2352055A1 (en) * | 1976-05-18 | 1977-12-16 | Mueller Hans | MICROORGANISMS RECYCLING PROCESS |
| EP0341736A1 (en) * | 1988-05-13 | 1989-11-15 | BIOCHEMIE Gesellschaft m.b.H. | Fertilizer comprising bacterial biomass, process for its production and its use as a special organic fertilizer |
| EP0369223A1 (en) * | 1988-10-27 | 1990-05-23 | Biochemie Gesellschaft M.B.H. | Bacterial biomass fertilizer |
| WO2018096429A1 (en) * | 2016-11-22 | 2018-05-31 | Solyom Zoltan | Procedure for preserving soil bacteria, suitably to produce preserved soil-improving bio-fertilizers |
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