GB709054A - Improved process for obtaining the circulatory hormone callicrein - Google Patents
Improved process for obtaining the circulatory hormone callicreinInfo
- Publication number
- GB709054A GB709054A GB20497/52A GB2049752A GB709054A GB 709054 A GB709054 A GB 709054A GB 20497/52 A GB20497/52 A GB 20497/52A GB 2049752 A GB2049752 A GB 2049752A GB 709054 A GB709054 A GB 709054A
- Authority
- GB
- United Kingdom
- Prior art keywords
- hormone
- aqueous
- pancreas
- temperature
- callicrein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6445—Kallikreins (3.4.21.34; 3.4.21.35)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The circulatory hormone callicrein is obtained from animal pancreas or submaxillary glands by forming an aqueous mixture of the animal substance, adjusting the hydrogen ion concentration of the mixture to pH 4 to 7, heating the aqueous mixture to 45 to 60 DEG C, and removing the aqueous component from the separated fatty and proteinaceous material. According to the example, fresh hog pancreas is minced, suspended in water, brought to a pH 4.6 by the addition of hydrochloric acid and stirred on e.g. a water bath until a temperature of 55 DEG C has been reached. The hot solution is centrifuged or allowed to stratify, and the aqueous solution removed. It is preferred to use the pancreas or submaxillary glands of the hog, and a thermolyis temperature of 55 DEG C, as in the example, when substantially all the hormone is liberated from the animal tissue within minutes of reaching this temperature. At 50 DEG C ten minutes is required, and at 48 DEG C one hour is required to ensure that the hormone is fully extracted into the aqueous phase.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE709054X | 1951-08-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB709054A true GB709054A (en) | 1954-05-12 |
Family
ID=6617773
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB20497/52A Expired GB709054A (en) | 1951-08-16 | 1952-08-14 | Improved process for obtaining the circulatory hormone callicrein |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB709054A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4817041B1 (en) * | 1970-05-06 | 1973-05-26 |
-
1952
- 1952-08-14 GB GB20497/52A patent/GB709054A/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4817041B1 (en) * | 1970-05-06 | 1973-05-26 |
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