GB2552110B - Methods of isolating nucleic acids under reduced degradation condition - Google Patents
Methods of isolating nucleic acids under reduced degradation conditionInfo
- Publication number
- GB2552110B GB2552110B GB1714375.1A GB201714375A GB2552110B GB 2552110 B GB2552110 B GB 2552110B GB 201714375 A GB201714375 A GB 201714375A GB 2552110 B GB2552110 B GB 2552110B
- Authority
- GB
- United Kingdom
- Prior art keywords
- methods
- under reduced
- nucleic acids
- isolating nucleic
- acids under
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000015556 catabolic process Effects 0.000 title 1
- 238000006731 degradation reaction Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/599,124 US20140039177A1 (en) | 2012-07-31 | 2012-08-30 | Methods of isolating nucleic acids under reduced degradation condition |
| GB1503908.4A GB2519910B (en) | 2012-08-30 | 2013-07-01 | Methods of isolating nucleic acids under reduced degradation condition |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| GB201714375D0 GB201714375D0 (en) | 2017-10-25 |
| GB2552110A GB2552110A (en) | 2018-01-10 |
| GB2552110B true GB2552110B (en) | 2018-05-23 |
Family
ID=50184574
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1503908.4A Expired - Fee Related GB2519910B (en) | 2012-08-30 | 2013-07-01 | Methods of isolating nucleic acids under reduced degradation condition |
| GB1714375.1A Expired - Fee Related GB2552110B (en) | 2012-08-30 | 2013-07-01 | Methods of isolating nucleic acids under reduced degradation condition |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1503908.4A Expired - Fee Related GB2519910B (en) | 2012-08-30 | 2013-07-01 | Methods of isolating nucleic acids under reduced degradation condition |
Country Status (2)
| Country | Link |
|---|---|
| GB (2) | GB2519910B (en) |
| WO (1) | WO2014035547A2 (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6544734B1 (en) * | 1998-10-09 | 2003-04-08 | Cynthia G. Briscoe | Multilayered microfluidic DNA analysis system and method |
| WO2004005553A1 (en) * | 2002-07-10 | 2004-01-15 | Massachusetts Institute Of Technology | Apparatus and method for isolating a nucleic acid from a sample |
| WO2012082849A1 (en) * | 2010-12-17 | 2012-06-21 | General Electric Company | Method for electroeluting genetic material from dried samples |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6120985A (en) * | 1997-10-31 | 2000-09-19 | Bbi Bioseq, Inc. | Pressure-enhanced extraction and purification |
| US6555945B1 (en) * | 1999-02-25 | 2003-04-29 | Alliedsignal Inc. | Actuators using double-layer charging of high surface area materials |
| WO2006132666A1 (en) * | 2005-06-06 | 2006-12-14 | Decision Biomarkers, Inc. | Assays based on liquid flow over arrays |
| US20070021735A1 (en) * | 2005-07-15 | 2007-01-25 | Sai Bhavaraju | Dual membrane electro-osmotic fluid delivery device |
| US20110097215A1 (en) * | 2009-10-23 | 2011-04-28 | The Government Of The United States Of America, As Represented By The Secretary Of The Navy | Flexible Solid-State Pump Constructed of Surface-Modified Glass Fiber Filters and Metal Mesh Electrodes |
-
2013
- 2013-07-01 GB GB1503908.4A patent/GB2519910B/en not_active Expired - Fee Related
- 2013-07-01 GB GB1714375.1A patent/GB2552110B/en not_active Expired - Fee Related
- 2013-07-01 WO PCT/US2013/048853 patent/WO2014035547A2/en not_active Ceased
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6544734B1 (en) * | 1998-10-09 | 2003-04-08 | Cynthia G. Briscoe | Multilayered microfluidic DNA analysis system and method |
| WO2004005553A1 (en) * | 2002-07-10 | 2004-01-15 | Massachusetts Institute Of Technology | Apparatus and method for isolating a nucleic acid from a sample |
| WO2012082849A1 (en) * | 2010-12-17 | 2012-06-21 | General Electric Company | Method for electroeluting genetic material from dried samples |
Also Published As
| Publication number | Publication date |
|---|---|
| GB2519910B (en) | 2018-05-09 |
| GB201714375D0 (en) | 2017-10-25 |
| WO2014035547A2 (en) | 2014-03-06 |
| GB201503908D0 (en) | 2015-04-22 |
| GB2519910A (en) | 2015-05-06 |
| WO2014035547A3 (en) | 2015-07-23 |
| GB2552110A (en) | 2018-01-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 20200701 |