GB2400559A

GB2400559A - Bacillus anthracis exoantigens

Info

Publication number: GB2400559A
Application number: GB0414537A
Authority: GB
Inventors: Mich Le Mock
Original assignee: Centre National de la Recherche Scientifique CNRS; Institut Pasteur
Current assignee: Centre National de la Recherche Scientifique CNRS; Institut Pasteur
Priority date: 1999-09-10
Filing date: 2000-09-08
Publication date: 2004-10-20
Anticipated expiration: 2020-09-08
Also published as: GB2398242B; GB0414537D0; GB2398242A; GB0414534D0; GB2400559B; GB0411571D0; GB2400558B; GB2400558A

Abstract

A purified antigenic preparation derived from Bacillus anthracis (anthrax) spores comprises one or more exoantigens of molecular weights 15 kDa, 30 kDa, 55 kDa or greater than 200 kDa. Antibodies directed against such preparations are also provided.

Description

wO 01/1;9395 2400559 ACELLuLAR IMMUNOGENIC coMposITIoN8 AND AC-uLAR

VACCINE COMPOSITIONS AGAINST EACILS ANTRACIS 1'

The present invention relates to acellular immunogenic compositions and also to acellular vaccine compositions against Bacillus anthracis, and to the uses thereof in human medicine and in veterinary medicine.

Bacillus anthracis (B. anthracis), the agent responsible for anthrax, or charbon, is an aerobic spore-forming Gram-positive bacterium.

This agent induces an infection either by intradermal inoculation or by ingestion or inhalation of the spores -- 15 (Klein F' et al., (1966), J. Infect. Dis., 116, 1213-138; ;Friedlander A.M. et al., (1993), J. Infect.

Die. 167, 1239-1242), the transformation of which into vegetative cells, encapsulated and toxinogenic forms, allows the bacterium to proliferate and to synthesize its virulence factors.

The inventors have recently shown, in a marine model of pulmonary infection with B. anthracis, that alveolar microphage are the primary site of the germination, which is rapidly followed by the expression of the toxin genes, confirming that the encounterbetween the spore and the host is crucial for the pathogenicity of B. anthracis (Guidi-Rontani E; et al., Holecular Biology, (1999), 31, 9-17).

The main virulence factors are: r_ the antiphagocytic capsule consisting of poly-y-D glutamic acid (Avakyan A.A. et al. (1965), J. of À Eacteriology, 90, 1082-1095) and - three protein factors which act in paired combination. The edematogenic toxin (PA-F) induces an edema after subcutaneous injection, whereas the lethal toxin (PA-F) is responsible - 2 - for animal death after intravenous injection (J.W. Ezzell et al., (1984), Infect. Immun., 45,

-

761-767). The factor present in both combinations is the protective antigen (PA) which is involved ' in the binding of toxins to the target cells. The other two factors, the edematogenic factor (OF) and the lethal factor (LF), are responsible for the manifestation of the toxic effect.

The simultaneous production of the capsule and of the of the toxins is essential for the manifestation of the pathogenic power.

The genes encoding the enzymes which synthesize the capsule are carried by the pX02 plasmid (Green B.D. et al., ( 1985), Infect. Tmmun., 49, 291297; Uchida I. et al. , (1985), J. Gen. Mcrobiol. , 131, 363-367) and the three genes peg, cya and lef, which encode, respectively, the PA, OF and LF factors, are carried by the pXO1 plasmid, which was described by Mikesell P. en al. (Infect. Immun, (1983), 39, 371-376).

Although many studies have shown that PA is the main antigen responsible for protection in The context of natural immunization or immunization acquired by vaccination, the inventors haste shown That LF is also a powerful immunogen (Mock M. Annales de l'Institut Pasteur [Annals of the Pasteur Institute] Dear 1990).

In order to clarify the role of the toxin components in the toxicity of B. anthracis, the inventors have constructed various mutants. Thus, they have characterized a strain which lacks the pX02 plasmid and lacks PA by modification of the pXO1 plasmid. Due to the absence of PA, this strain is no longer lethal in nature (Cataldi A. et al. (1990), Molecular Microbiology, 4, 1111-1117). - 3 -

In order to investigate the elements which may be involved in immunization against infection with B. anthracis, the inventors have constructed mutants lacking at least one of the toxicity factors responsible for pathogenicity, i.e. deficient in PA, in EF or in LF, or even lacking the pXO1 plasmid and also lacking the pX02 plasmid. Although lacking toxicity or exhibiting attenuated toxicity, the single mutants, in particular RP9 (EF-) (Collection Nationale de Cultures et de Microorganismes [National Collection of Cultures and of Microorganisms] held by the Institut Pasteur under the number I-1094, dated Nay 2, 1991) and RP10 (LF-) (Collection Nationale de Cultures et de Microorganismes held by the Institut Pasteur under the number I-1095, dated Nay 2, 1991), and the double mutant RP 42 (Collection Nationale de Cultures et de Microorganismes held by the Institut Pasteur under the number I-2271, dated July 28, 1999) proved to be capable of producing antibodies immunoprotective against infection with a wild-type Sterne strain. These mutants are described in international application No. 92/19720, and in the articles by C. Pezard et al., (Infection and Immunity, (1991), 59, 3472-3477 and J. General Microbiology, (1993), 139, 2459-2463).

Currently, the veterinary vaccine Marketed (Merial.) is a live vaccine composed of a suspension of spores of the Sterne strain of B. anthraci-. Its protective efficacy in animals varies depending on the batch, without it being possible to determine the causes of these variations.

This random efficacy, side effects and also the potential risk of disseminating live germs in the environment make its use in humans impossible.

In human medicine, two vaccines against anthrax, essentially developed in Great Britain and in the United States, are used. They are acelullar vaccines - 4 - consisting mainly of the protective antigen (PA), prepared from culture supernatants of the toxinogenic Sterne strain of B. anhracis, and of an adjutant which can be used in human medicine, aluminum hydroxide. ' Recent studies on these two vaccines have shown that ' the British vaccine, containing traces of EP and of OF which induce an antibody response by ELISA, is more efficacious in guinea pigs than the American vaccine, which apparently lacks these two components (Turnbull P.C. et al., (1991), Vaccine, 9, 533-539).

However, these two vaccines have a certain number of drawbacks: - the vaccination protocol is restrictive, since it requires six injections in eighteen months, followed by one booster per year, - they induce harmful side effects which limit their use, - the protection induced by these acellular vaccines in animals, against a challenge with a virulent strain, is never complete, unlike that obtained with the live vaccine.

Given the magnitude of the infections caused by B. anthracis, many studies are currently dedicated to improving the vaccine so that it does not have the drawbacks set out above, but at the same time exhibits the same protection as the live vaccine.

In this context, the inventors have given themselves the aim of providing a reliable efficacious acellular; vaccine free of side effects which overcomes the drawbacks of the existing vaccines and the vaccine properties of which are easy to control.

Consequently, a subject of The present invention is an acellular immunogenic composition capable of inducing an immune response against B. anthracis infections, - s - characterized in that it comprises: - a protective antigen (PA), - killed, optionally purified, spores obtained either from mutant strains of B. anthracis carrying one or more mutations chosen from mutations in at least one gene encoding a protein responsible for a toxic effect, in B. anhracis, or from mutant strains of B. anthracis lacking at least one of the pXO1 and pX02 plasmids, combined at least with a pharmaceutically acceptable vehicle.

In an advantageous embodiment of the invention, said acellular immunogenic composition is capable of producing antibodies against B. anthracis.

A subject of the present invention is also an acellular vaccine composition against B. antacid, characterized in that it comprises: - a protective antigen (PA), - killed, optionally purified, spores obtained either from mutant strains of B. anthracús carrying one or more mutations chosen from mutations in at least one gene encoding a protein responsible for a toxic effect, in B. anthracis, or from mutant strains of B. anthracis lacking at least one of the pXO1 and pX02 plasmids, combined at least with a pharmaceutically acceptable vehicle and with at least one adjuvant.

For the purpose of the present invention, the Metal.

Cellular means that the immunogenic or vaccine composition no longer contains any viable cells (killed spores).

The adjutants used are adjutants conventionally used and will, in particular, be either saponin, in the case of the veterinary vaccine, or advantageously chosen from the group consisting of aluminum hydroxide and - 6 - squalene, in the case of the human vaccine.

In the context of the present invention, the spores may be killed by any physical or chemical means which leads to their inactivation. By way of example, mention may be made of treatment with formaldehyde or irradiation.

For the purpose of the present invention, the term mutation. is intended to mean a deletion, modification or addition in the gene concerned, which results in a gene either lacking its ability to produce the corresponding protein or capable of producing an "inactive protein.

According to a particular embodiment of the invention, the immunogenic compositions and the vaccine compositions may also comprise at leant one detoxified exotoxin chosen in particular from the group consisting of the lethal factor (LF) and the edematogenic factor (EF), which have been detoxified, i e. which have lost their toxic properties.

These inactivated protein factors may in particular be obtained by expressing the genes which have been mutated in the sequence encoding the active site of said protein factors (pya or lef).

The immunogenic and vaccine compositions according to the invention have, surprisingly, a strong protective capacity, of the order of 100%, which is clearly greater than that obtained with the PA alone or the killed spores alone, which makes it possible to obtain complete immunization with a single injection under the conditions for the veterinary vaccine, and two injections under the conditions for the vaccine for human use.

According to another advantageous embodiment of the immunogenic and vaccine compositions according to the invention, the spores are derived from a strain of B. anthracis chosen from the group consisting of the following strains: Sterne 7702 (N. Sterne J. Vet. Sci. Enema. Indust., (1939), 13, 315-317), RPLC' (Collection Nationale de Cultures et de Microorganismes held by the Institut Pasteur under the number I-2270, dated July 28, 1999) and RP42 (Collection Nationale de Cultures et de Microorganismes held by the Institut Pasteur under the number I-2271, dated July 28, 1999).

In another advantageous embodiment of the immunogenic and vaccine compositions according to the invention, the protective antigen is chosen from the group consisting of the purified protective antigens derived from any wild-type or mutated Sterne strain of B. anthracis, and the recombinant protective antigens, in particular that produced by B. subtilis.

Advantageously, the protective antigen is derived from the RP42 strain (Collection Nationale de Cultures et de Microorganismes held by the Institut Pasteur under the number I-2271, dated July 28, 1999).

The subject of the present invention is also the RPC2 Strain deposited with the Collection Nationale de Culture. et de Microorganismes he'd at the Institut Pasteur under the number I-2270, dated July 28, 1999).

A subject of the present invention is also the use of at least one antibody directed against the spores derived from strains obtained either from mutant strains of B. anthracis carrying one or more mutations chosen from mutations in at least one gene encoding a protein responsible for a toxic effect, in B. anthracis. or from mutant strains of B. anthracis lacking at least one of the pXOl and pX02 plasmids, for producing a medicinal product capable of inducing passive immunization. In fact, antibiotics are the only current treatment against anthrax and must be - 8 - administered early, before the appearance of the toxic shock. Consequently, a serotherapy aimed at both the toxins and the spore germination would be a good addition. -.

The antibodies may be polyclonal antibodies obtained by immunizing a suitable animal with the spores derived from strains used for preparing the compositions according to the invention, under conventional conditions for preparing such antibodies.

The antibodies may be monoclonal antibodies obtained in a.way known per se, in particular by fusing Spleen cells from mice immunized with an antigen consisting or spores derived from strains used for preparing The compositions according to the invention.

A subject of the present invention is also purified antigenic preparations, characterized in that they ore derived from B. anthracis spores and Comprise, for example, one or more of the exoantigens (proteins of spores and of the exosporium) of respective molecular weights 15 kDa, 30 kDa, 55 kDa, and greater than kDa, said molecular weights being determined using the AIRS LOW Electroporesis Calibration Kit.

In accordance with the invention, the antigenic compositions are obtained by conventional techniques known to those skilled in the art.

The subject of the present invention is also the polyclonal or monoclonal antibodies directed against said antigen compositions.

The immunogenic and vaccine compositions according to the invention may be administered alone or in combination with other vaccines, by injection or by any route conventionally used for vaccination. i - 9 -

The doses to be administered will be determined depending on the animal or the person for whom protection is being sought.

The invention also includes the following aspects: Aspect 1 An acellular immunogenic composition capable of inducing an immune response against B. anthracis infections, characterized in that it comprises: - a protective antigen (PA), - killed, optionally purified, spores obtained either from mutant strains of B. anthracis carrying one or more mutations chosen from mutations in at least one gene encoding a protein responsible for a toxic effect, in B. anthracis or from mutant strains of B. anthracis lacking at least one of the pX01 and pX02 plasmids, combined at least with a pharmaceutically acceptable vehicle.

Aspect 2 The acellular immunogenic composition of Aspect 1, characterized in that it is capable of producing antibodies against B. anthracis.

Aspect 3 An acellular vaccine composition against B. anthracis, characterized in that it comprises: - a protective antigen (PA), - killed, optionally purified spores, obtained either from mutant strains of B. anthracis carrying one or more mutations chosen from mutations in at least one gene encoding a protein responsible for a toxic effect, in B. anthracis, or from mutant strains of B. anthracis lacking at least one of the pX01 and pX02 plasmids, combined at least with a pharmaceutically acceptable vehicle and with at least one adjuvant. -

Aspect 4 The immunogenic composition of either of Aspects 1 and 2, or the vaccine composition of Aspect 3, characterized in that it also comprises at least one detoxified exotoxin chosen from the group consisting of the lethal factor (LF) and the edematogenic factor (EF), which have been detoxified.

Aspect 5 The immunogenic compositions of either of Aspects 1 and 2, or the vaccine composition of Aspect 3, characterized in that the spores are derived from a strain of B. anthracis chosen from the group consisting of the following strains: Sterne 7702, RPLC2 (Collection Nationale de Cultures et de Microorganismes [National Collection of Cultures and of Microorganisms] held by the Institut Pasteur under the number 1 2270, dated July 28, 1999) and RP42 (Collection Nationale de Cultures et de Microorganismes held by the Institut Pasteur under the number 1-2271, dated July 28, 1999).

Aspect 6 The immunogenic composition or vaccine composition of any one of Aspects 1 to 5, characterized in that the protective antigen is chosen from the group consisting of the purified protective antigens derived from any wild-type or mutated Sterne strain of B. anthracis, and the recombinant protective antigens.

Aspect 7 The immunogenic composition or vaccine composition of Aspect 6, characterized in that the protective antigen is derived from the RP42 strain (Collection Nationale de Cultures et de Microorganismes [National Collection of Cultures and of Microorganisms] held by the Institut Pasteur under the number 1-2270, dated July 28, 1999).

Aspect 8 The RPLC2 strain deposited with the Collection Nationale de Cultures et de Microorganismes "National Collection of Cultures and of Microorganisms) held at the Institut Pasteur under the number 1-2270, dated July 28, 1999. - 11

Aspect 9 The use of at least one antibody directed against the spores derived from strains obtained either from mutant strains of B. anthracis carrying one or more mutations chosen from mutations in at least one gene encoding a protein responsible for a toxic effect, in B. anthracis, or from mutant strains of B. anthracis lacking at least one of the pXO1 and pX02 plasmids, for producing a medicinal product capable of inducing passive immunization.

Aspect 10 A purified antigenic preparation, characterized in that it is derived from B. anthracis spores and comprises one or more of the exoantigens of respective molecular weights 15 kDA, 30 kDA, 55 kDA, and greater than 200 kDA.

Aspect 1 1 An antibody directed against the antigenic preparations of Aspect 10.

Other ch'acteristics an.d advantages of the invention appear in the remainder of the description And examples illustrated ky,'the figurer in which: ..

- figure 1 represents the immnoblot analysis of the spore proteins according to. the procedure.

described in.example 5,'' - figure 2 represents,the immNnoblot Analysis of the exosporium proteins (A) revelation with a polyclonal antibody.and a monoclinal antibody (35B8) (B? analysis' according, to.the procedure described in example 5, ' ' -' figure 3. represents the various strains of ' " B. an.+hacds UP" to.pr e the RPLC2 strain. The ' ..

RPLC2 strain produces th toxin oonpcnente : inactivated by point mutations in the active sites.

of the LF (LF686; H686-ta) and ED (nF346.353; R346-HQ and K3s3mQ? protean. Ih.this figure,, The numbers which follow indicate the nucleotidss at which the deletions tee yin and end; term, Ken 'and, . 8p8 indicate the insertion of erythromycin resist--cc, kanaóycin resistance and spectinomycin resistance cassettes; 0 correct. EVER to an organism which Hal no resistance to these antibiotics.

EXAMPLE he aeter4els -d methods for Reps the campositios according to the Invention 1.1. Construction of the RPC2 strain

_ _ _

The RPCL2 strain (Collection Rationale de Cultures et de NicroorganismeS held by the Institut Pasteur under the number I-2270, dated July 28, 1999) is constructed from the strains indicated in figure 3, according to the operating principles described by C. Pezard et al. (1993) (reference cited).

1.2 Preparation of PA The PA protein is prepared from the mutant i. anthracis strain RP42 (Collection Nationale de Cultures et de Microorganismes [National Collection of Cultures and of Microorganisms] held by the Institut Pasteur under the number I-2271, dated July 28, 1999) . . The medium R culture supernatantn (Riatroph J. D. et al. (1983) Infection and unity, 39, 483-486)- are filtered and then concentrated on a Minitan. system (Millipore. PLGC ONP membrane). . ..

The -PA-:antigen - is --then Purified by---ultra-rapid chromatography (FRANC) on a monoQ column according to the protocol described by Pezard C. et al. (1993) (reference cited). . 1.3. Preparation and inactivation of_pores.

The spores are prepared from the Sterne ?702 strain according to the procedure described by E. Guidi-anatani et al. (1999) (reference cited).

The Spores are prepared on a solid NAY medium and then washed with distilled water. They are Activated ty treatment with formal, at a final concentration of 4t, for 3 hours at 3? C. . After washing by eentrifugation, the ep.ores-are taken up in the initial volume of physiological saline (final concentration of lO9 spores/ml).

This suspension is used to perform the immunization.

If necessary' in particular when the intention is to prepare a vaccine for human use, the spores may be purified before the formal treatment, on a 50% to 76% gradient of Radioseleetran. (& hewing S..).

1.4 Preparation of the vaccine coE06ition The compositions are prepared e:her from killed spores alone, prepared according to the procedure described in: 1.3, or from a mixture of PA (at a concentration such that 10 fig per mouse are injected) and of killed spores.

(108 spores per mouse), to which either aluminum: hydroxide at a final concentration of O.3% or saponin Àat a final concentration of 0.05% is added as an.

adjutant. . 1.5 Protocol for treating mice.

Six-week-old female Swim- mice supplied by the company Iffa-Credo (BP010269592 L'ARBRBSLE-Cedex) are used.

The animals are divided up into group. of six and fed.

ad libitum. . The injection. are. given subcutaneously into The groin, in a volume of 200 1. . . . . _. . . . . 1.6. Titering antibody levels: The antibody levels are titered using a conventional ELI&A assay.

EXAMPLE as Effect.of two mmunisetoDe Is the condition for the homes ecallular Vaccine (protocol To. 1) 2.1. Treatment of animals. I The injection protocol for each group is as follows: - two injections of vaccine compositions prepared as indicated in point 1. 4. or of adjuvant (aluminum hydroxide) are given 28 days apart and.

- a challenge injection is given on the 43rd day, with the virulent B. anthracis strain 17JB (Pasteur reference strain No. 2) provided by The company Rh8ne-Merieux.

Four groups of animals are immunized according to this protocol as follows: . -15- . - the first group receives the aluminum hydroxide alone (control group), À - the second group receives a PA dose of lo fig per mouse, . - the third group receives the spores alone, at Gus spores per mouse, and - the fourth group receives the PA + killed spores mixture no as to have lo fig or PA and l0B spores per mouse.

All the groups receive, on the 43rd day, as specified above, a challenge dose corresponding to 30 tames the: - - LD50, i.e. l.S x 104 spores per mouse.

2.2. Results À The survival rates are given in table I below.

À . TAB1R I _.

Treatment Number of. Percentage deathe at the survival tat the 43rd day. . 43rd day.

Adj want alone 6J6. , 08.. . PA alone 3/6 50' Killed spore. alone 2/6 33% PA killed spores 0/6 100% These results clearly show that only - vaccine compositions according to the invention are capable or allowing complete protection.

XANPIR as Effect of the;=mm4zatons under the conditions for the vacDe for hen use (protocol No. 2) 3.1. Treatment of animals The injection protocol for each group is as follows: - two injection of vaccine compositions prepared as indicated in point 1. 4. or of adjuvant (aluminum hydroxide) are gives 21 days apart, and - 16 - - , ' . - a challenge injection is given on the 32nd day, ! with the virulent B. anhracis strain 17JB (Pasteur reference strain No. 2) provided by the company RhOne-Merieux.

Four groups of animals are immunized according to this protocol a. follows: - the first group receives the aluminum hydroxide alone, - the second group receives a PA dose of lo fig per mouse, . I - the third group receives the spores alone, at 108 spores per mouse, and - the fourth group receives the PA killed spores mixture so as to have to fig of PA and 108 spores per mouse.

All the groups receive. on the 32nd day, as Specified above, a challenge dose correpon;ng to 100 times the D50, i.e. 1.5 x 104 spore" per mouse.

3.2. Results 3.2.1. Survival rated The results are given in table II below

TABLE II

Treatment Thurber of Percentage deaths at the survival at the 32nd day __ 32nd day Adjuvant alone; 6/6 0% PA alone 1/6 83.

Killed spores alone 1/7 85%

__ _

PA + killed spores 0/6 loot These results clearly show that only the vaccine compositions according to the invention are capable of allowing complete protection. - 17

3.2.2. Antibody levels The-levels of antibodies directed against The spores À are high, of the order. of 10 000 to 15 00G, and identical in the two groups which received them, whether these spores are alone or combined with PA.

These.results confirm the synergistic effect of The compositions according to the invention, which, with an Antibody level identical to that obtained by injecting 10.the killed spores alone, allows complete projection.

EAMp.e._4.s a.o._si Jih_u Lacy of....h-_YaOoDe: À composite co accorded. t- the enroot. on with the steno lit rack, under the cozen t' ok for the racco for Ureters uee (a Mingle izijectan unto a an t" "jlZVt) challen5 "th the 17J atr-4n 4.1. Treatment ofLanmele.

The injection protocol for each group is as follows: - one -injection of vaccine composition prepared as indicated in point 1. 4. or of saponin to given an À .DO, and. . - challenge injection is given on the 32nd day, with the virulent B. anhace Strain 37JB (Pasteur reference No. 2) provided by the company Rh8ne-Merieux.

Five groups of animals are immunized according to this protocol as follows: - the first group receives saponin alone (control group), - the second group receives a PA dose of 10 fig per mouse, - the third group receives the spores alone, at 108 spores per mouse, - - the fourth group receives the PA + killed spores mixture Do as to have 10 fig of PA and 108 spores per mouse, and - the fifth group receives the Sauterne live vaccine -18- : prepared at the Institut Pasteur.

All the groups receive a challenge dose corresponding to log tomes the LD50, i.e. 105 spores, on the 32nd day.

4.2. Results - - They are given in Table III below.

TABLE III

Treatment dumber of Percentage deaths at the Survival at the - 32nd day 32nd dav

_

Adjuvant alone _ 6./6 0% PA alone. l/6. -83% Live spores, O/S. . 100% Billed pore. alone 4/6 33. . PA + killed spored 0/6.. look.

These results clearly show that the vaccine compositions according. to the invention are as À efficacious as the live.vaccne and mmy, consequently, advantageously be used as a veterinary vaccine.

EXANP 5' Allot analysts of the B. -Nabisco spore proteDs 5.1. Materials and methods 5.l.l. Preparation of the pollonal and monoclinal antibodies A polyclonal serum from mice immunized with killed spores derived, for example, from the RPLC2 strain (Collection Nationale de Cultures et de Microorganismes [National Collection of Cultures and of Microorganisms] held by the Institut Pasteur under the number I-2270, dated July 28, 1999) is prepared according to conventional techniques known to those skilled in The art The monoclonal antibody specific for the spore surface (35B8) is prepared according to the technique described by Kohler et al. (1975), Nature, 296, 495-497.

5.1.2. Irtraction of the spores The proteins from the spore are solubilized by treating the spores with a Tris-HC1 buffer, at pH 9.8, containing EM of urea and 2% of SDS, or with a 10 me Tris buffer at pH 9. 5, containing 10 me of Ev1A Id 1.

of SDS, according to the technique described by _Garcia-Patrone (1995), Holecular and Cellular Eioybe., =5,._29.-31- _ _ _ __ _._. _._ -_ _.. _ _ 15. 5.2. Results. . ..

They are illustrated in figure 1 and figure 2. . The mouse polyclonal serum recognizes 3 protein Species of repective..moleculr.weishts lS.kDa, 30 kDa, 5S kDa and a protean.specie-.of molecular weight greater t ban kid (figure 1).

The heaviest Species is also. recognized by The.

monoclinal antibody 35B8 and appears to belong to the I exoporium (figure 2A).

Specifically. the immunoblot analysis of ha exosporium proteins _show" the t the various monoclinal antibodies used, including 35B8, recognize a protein species of molecular weight greater the" 200 kDa (figure 2A).

It emerges from the -above that the vacc; no compositions according to the invention are capable of allowing complete protection both under the conditions for the human vaccine and under the conditions for the veterinary vaccine. . . . EXAMPLE 6: Comparison of The efficacy of thevaccine compositions according to the invention aa-n4atoro according to protocol Do. of example 2, with the: HA antig-" alone, in Deco or in guinea Dices challenge with the 9602 Strom A. Swiss mice 6.1 Treatment of animals: . The injection protocol for each coup is as follows: - two injections of the vaccine compositions prepared as indicated in point 1.4 in example 1 are given 15 days apart.(DO P"d D15), and.

- a challenge injection i. given on 35th day, with the virulent strain 9602 (M. Berthier et al., Lancet, i996, 347, 9004:828j isolated from a lethal cane of human anthrax, and the virulence of which is,ten times greater than that of the 17JB "train used in the previous examples; said strain i" injected subcutaneously. , groups o,f animals an defined in example 2 are immunized according to this protocol. ' All the groups receive, on the 35th day, as specified. ; above. a challenge dose corresponding to 30 times the LD50, i.e. 1.5 x 104 spore. per mouse. ' ' I 6.2. Results - The experiment. were repeated 3 times, with different preparations. on batches of 6 to 8 mice per point (due 30.to Pa containment). i The survival rates are illustrated in table rv below. T,

_

Treatment Percentage survival at the 35th day and up to the 43rd day Adjuvant alone. 0% ., PA alone. 0% Killed spores alone. 0% a.

PA killed spores 100t B. Guinea pig=.

The-experiments were carried out twice, on batches of 6 guinea pigs. The protocol in similar to that used in the mice, with the exception of the following points: - The PA doses are 40 g per animal, - . the challenge injection-is given intramuacuiarly.

1008 survival is obtained for the combination according À to the invention, which id killed. sported PA versus 40% in the animals receiving PA alone, which is Ale Imposition of the-- conventional vaccine.

6.3. Antibody levels.

These experiments (mice. and guinea High) were accompanied by nn";toring of the antibody response by ELISA on serum samples from mice and from guinea pigs.

The anti-PA antibody titers are high (> 5 000); a À20 response of the same order i" det' Ted against pore- specific antigens.

EXANPn 7s Compr$oon of the efficacy of the Veaoio.

compositions accord$ao to the invention with the Bterne live vaccine, under the coDitlona for the =.cc- tor vetorny use as described An en y ple 4 (challenge with the 9602 strain) . The test was carried out on Swiss mice (under the conditions described in example 4). m e challenge injection is given with the 9602 strain (M.-Berhier.et al., mentioned above), to mice which have received a single injection either of live spores (APACE) or.of the combination according to the inven-tion,- which is killed spores + PA. The protection efficacy, 83%, is identical for both batches.

These results clearly show that it is possible to provide 100% protection of mice and guinea pigs with a vaccine combination comprising killed spores and Ace PA antigen. - - 23

Claims

CLAIMS: 1. A purified antigenic preparation, characterized in that it is

derived from B. anthracis spores and comprises one or more of the exoantigens of respective molecular weights 15 kdA, 30 kDA, 55kDA, and greater than 200 kDA.
2. An antibody directed against the antigenic preparations as claimed in Claim 1. À , ( , c < c I, c,

2. An antibody directed against the antigenic preparations as claimed in Claim Amendments to the claims have been filed as follows CLAIMS: 1. A purified antigenic preparation, characterized in that: - it is obtained by selecting protein species of respective molecular weights 15 kDa, 30 kDa, 55 kDa and a protein species of molecular weight greater than 200 kDa recognised by a mouse polyclonal serum obtained by immunization of said mice with killed spores of mutant strains of B. anthracis carrying one or more mutations chosen from mutations in at least one gene encoding a protein responsible for a toxic effect, in B. anthracis or from mutant strains of B. anthracis lacking at least one of the pX01 and pX02 plasmids and - in that it consists essentially of one or more of the exoantigens of respective molecular weights 15 kDa, 30 kDa, 55 kDa, and greater than 200 1 5 kDa.