GB2485850C - Methods and compositions for detecting copy number and chromosome aneuploidy by ligation probes and partitioning the ligated products prior to amplification - Google Patents
Methods and compositions for detecting copy number and chromosome aneuploidy by ligation probes and partitioning the ligated products prior to amplificationInfo
- Publication number
- GB2485850C GB2485850C GB1100787.9A GB201100787A GB2485850C GB 2485850 C GB2485850 C GB 2485850C GB 201100787 A GB201100787 A GB 201100787A GB 2485850 C GB2485850 C GB 2485850C
- Authority
- GB
- United Kingdom
- Prior art keywords
- partitioning
- amplification
- compositions
- methods
- copy number
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000003321 amplification Effects 0.000 title 1
- 208000036878 aneuploidy Diseases 0.000 title 1
- 231100001075 aneuploidy Toxicity 0.000 title 1
- 210000000349 chromosome Anatomy 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- 238000003199 nucleic acid amplification method Methods 0.000 title 1
- 239000000523 sample Substances 0.000 title 1
- 238000000638 solvent extraction Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (13)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26459109P | 2009-11-25 | 2009-11-25 | |
| US30983710P | 2010-03-02 | 2010-03-02 | |
| US30984510P | 2010-03-02 | 2010-03-02 | |
| US31763510P | 2010-03-25 | 2010-03-25 | |
| US31763910P | 2010-03-25 | 2010-03-25 | |
| US34121810P | 2010-03-25 | 2010-03-25 | |
| US31768410P | 2010-03-25 | 2010-03-25 | |
| US34106510P | 2010-03-25 | 2010-03-25 | |
| US38098110P | 2010-09-08 | 2010-09-08 | |
| US40910610P | 2010-11-01 | 2010-11-01 | |
| US10947310P | 2010-11-02 | 2010-11-02 | |
| US41076910P | 2010-11-05 | 2010-11-05 | |
| PCT/US2010/058124 WO2011066476A1 (en) | 2009-11-25 | 2010-11-25 | Methods and compositions for detecting genetic material |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| GB201100787D0 GB201100787D0 (en) | 2011-03-02 |
| GB2485850A GB2485850A (en) | 2012-05-30 |
| GB2485850B GB2485850B (en) | 2014-01-29 |
| GB2485850C true GB2485850C (en) | 2019-01-23 |
Family
ID=44066935
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1100787.9A Active GB2485850C (en) | 2009-11-25 | 2010-11-25 | Methods and compositions for detecting copy number and chromosome aneuploidy by ligation probes and partitioning the ligated products prior to amplification |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN102985552B (en) |
| CA (1) | CA2767028A1 (en) |
| GB (1) | GB2485850C (en) |
Families Citing this family (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA2881685C (en) | 2012-08-14 | 2023-12-05 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| EP2931919B1 (en) | 2012-12-14 | 2019-02-20 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP2954104B1 (en) | 2013-02-08 | 2020-09-16 | 10X Genomics, Inc. | Polynucleotide barcode generation |
| WO2014172288A2 (en) * | 2013-04-19 | 2014-10-23 | Raindance Technologies, Inc. | Digital analyte analysis |
| EP3024948B1 (en) * | 2013-07-25 | 2020-01-15 | Bio-rad Laboratories, Inc. | Genetic assays for detecting viral recombination rate |
| US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
| CN104169929B (en) * | 2013-09-10 | 2016-12-28 | 深圳华大基因股份有限公司 | For determining system and the device of fetus whether existence numerical abnormalities of chromosomes |
| US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
| DE202015009609U1 (en) | 2014-04-10 | 2018-08-06 | 10X Genomics, Inc. | Microfluidic system for the production of emulsions |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| JP2017526046A (en) | 2014-06-26 | 2017-09-07 | １０エックス ゲノミクス，インコーポレイテッド | Nucleic acid sequence assembly process and system |
| JP2017522866A (en) | 2014-06-26 | 2017-08-17 | １０エックス ジェノミクス， インコーポレイテッド | Nucleic acid sequence analysis |
| EP4574974A3 (en) | 2014-06-26 | 2025-10-08 | 10x Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| CN104032031A (en) * | 2014-07-04 | 2014-09-10 | 华东理工大学 | PCR analyzing method for quantitatively detecting nucleic acid through RNA polymerase and ligase coupled reaction medium |
| CN114807307A (en) | 2014-10-29 | 2022-07-29 | 10X 基因组学有限公司 | Methods and compositions for sequencing target nucleic acids |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| BR112017014902A2 (en) | 2015-01-12 | 2018-03-13 | 10X Genomics Inc | processes and systems for the preparation of nucleic acid sequencing libraries and libraries prepared using them |
| SG11201705425SA (en) | 2015-01-13 | 2017-08-30 | 10X Genomics Inc | Systems and methods for visualizing structural variation and phasing information |
| MX2017010142A (en) | 2015-02-09 | 2017-12-11 | 10X Genomics Inc | Systems and methods for determining structural variation and phasing using variant call data. |
| EP4286516A3 (en) | 2015-02-24 | 2024-03-06 | 10X Genomics, Inc. | Partition processing methods and systems |
| JP2018505688A (en) | 2015-02-24 | 2018-03-01 | １０エックス ゲノミクス，インコーポレイテッド | Method for targeted nucleic acid sequence coverage (COVERAGE) |
| CN105936930A (en) * | 2015-03-04 | 2016-09-14 | 松下知识产权经营株式会社 | DNA detection method and DNA detection device |
| CN105969655A (en) * | 2015-03-10 | 2016-09-28 | 松下知识产权经营株式会社 | Method for analyzing multiple nucleic acid targets |
| EP3329010B1 (en) * | 2015-07-29 | 2019-07-10 | Progenity, Inc. | Nucleic acids and methods for detecting chromosomal abnormalities |
| KR101848438B1 (en) | 2015-10-29 | 2018-04-13 | 바이오코아 주식회사 | A method for prenatal diagnosis using digital PCR |
| WO2017074094A1 (en) * | 2015-10-29 | 2017-05-04 | Biocore Co., Ltd. | A method for prenatal diagnosis using digital pcr. |
| KR20240161696A (en) | 2015-12-04 | 2024-11-12 | 10엑스 제노믹스, 인크. | Methods and compositions for nucleic acid analysis |
| EP3414341A4 (en) | 2016-02-11 | 2019-10-09 | 10X Genomics, Inc. | SYSTEMS, METHODS, AND MEDIA FOR ASSEMBLING NOVO OF GENOME SEQUENCE DATA OVERALL |
| CN105821482B (en) * | 2016-04-29 | 2018-04-10 | 李星军 | A kind of biochemistry micro- reaction system, high-flux sequence build storehouse instrument and application |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CN110214186B (en) | 2017-01-30 | 2023-11-24 | 10X基因组学有限公司 | Methods and systems for droplet-based single cell barcoding |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| WO2018213774A1 (en) | 2017-05-19 | 2018-11-22 | 10X Genomics, Inc. | Systems and methods for analyzing datasets |
| EP4230746A3 (en) | 2017-05-26 | 2023-11-01 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| US20180340169A1 (en) | 2017-05-26 | 2018-11-29 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| CN107619827B (en) * | 2017-09-12 | 2020-11-13 | 广州阳普医疗科技股份有限公司 | Composition, preparation method thereof and application thereof in field of preservation of free DNA (deoxyribonucleic acid) in whole blood |
| WO2019099751A1 (en) | 2017-11-15 | 2019-05-23 | 10X Genomics, Inc. | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| CN112262218B (en) | 2018-04-06 | 2024-11-08 | 10X基因组学有限公司 | Systems and methods for quality control in single cell processing |
| EP3781710A4 (en) * | 2018-04-17 | 2022-01-05 | Chromacode, Inc. | MULTIPLEX ANALYSIS METHODS AND SYSTEMS |
| EP4104179B1 (en) | 2020-02-13 | 2026-01-28 | 10X Genomics, Inc. | Systems and methods for joint interactive visualization of gene expression and dna chromatin accessibility |
| WO2021252733A2 (en) * | 2020-06-10 | 2021-12-16 | Bonadea Diagnostics, Llc | Sequence conversion reaction |
| CN116153393B (en) * | 2023-02-23 | 2025-12-02 | 宁波市妇女儿童医院 | A method for chromosome copy number counting based on relative fluorescence intensity between chromosome monochromatids |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7537897B2 (en) * | 2006-01-23 | 2009-05-26 | Population Genetics Technologies, Ltd. | Molecular counting |
| ES2446927T3 (en) * | 2006-03-01 | 2014-03-10 | Keygene N.V. | Rapid sequence-based SNP detection using ligation assays |
| EP2021113A2 (en) * | 2006-05-11 | 2009-02-11 | Raindance Technologies, Inc. | Microfluidic devices |
| CA2655272C (en) * | 2006-06-14 | 2017-04-18 | Living Microsystems, Inc. | Rare cell analysis using sample splitting and dna tags |
| GB2502409B8 (en) * | 2008-09-23 | 2014-10-15 | Bio Rad Laboratories | Droplet-based assay system |
| US9156010B2 (en) * | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
-
2010
- 2010-11-25 CA CA2767028A patent/CA2767028A1/en not_active Abandoned
- 2010-11-25 GB GB1100787.9A patent/GB2485850C/en active Active
- 2010-11-25 CN CN201080062146.9A patent/CN102985552B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| GB201100787D0 (en) | 2011-03-02 |
| GB2485850B (en) | 2014-01-29 |
| CN102985552A (en) | 2013-03-20 |
| CN102985552B (en) | 2016-02-17 |
| CA2767028A1 (en) | 2011-06-03 |
| GB2485850A (en) | 2012-05-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| COOA | Change in applicant's name or ownership of the application |
Owner name: BIO-RAD LABORATORIES, INC. Free format text: FORMER OWNER: BIO-RAD QL, INC |
|
| S73 | Revocation on comptroller's initiative (section 73/patents act 1977) |
Free format text: SPECIFICATION AMENDED |