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GB2473401B - Recombinant bacteria for producing deoxyviolacein and uses thereof - Google Patents

Recombinant bacteria for producing deoxyviolacein and uses thereof

Info

Publication number
GB2473401B
GB2473401B GB1100438.9A GB201100438A GB2473401B GB 2473401 B GB2473401 B GB 2473401B GB 201100438 A GB201100438 A GB 201100438A GB 2473401 B GB2473401 B GB 2473401B
Authority
GB
United Kingdom
Prior art keywords
recombinant bacteria
deoxyviolacein
producing
producing deoxyviolacein
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB1100438.9A
Other versions
GB201100438D0 (en
GB2473401A (en
Inventor
Xinhui Xing
Peixia Jiang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
STAR LAKE BIOSCIENCE CO Inc
Tsinghua University
Original Assignee
STAR LAKE BIOSCIENCE CO Inc
Tsinghua University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN2008101166013A external-priority patent/CN101319219B/en
Priority claimed from CN2008102243591A external-priority patent/CN101368169B/en
Application filed by STAR LAKE BIOSCIENCE CO Inc, Tsinghua University filed Critical STAR LAKE BIOSCIENCE CO Inc
Publication of GB201100438D0 publication Critical patent/GB201100438D0/en
Publication of GB2473401A publication Critical patent/GB2473401A/en
Application granted granted Critical
Publication of GB2473401B publication Critical patent/GB2473401B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
    • C12P17/165—Heterorings having nitrogen atoms as the only ring heteroatoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/182—Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system
    • C12R1/19—
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
GB1100438.9A 2008-07-11 2009-04-22 Recombinant bacteria for producing deoxyviolacein and uses thereof Expired - Fee Related GB2473401B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2008101166013A CN101319219B (en) 2008-07-11 2008-07-11 A method for producing deoxyviolet bacterin and its special recombinant bacteria
CN2008102243591A CN101368169B (en) 2008-10-17 2008-10-17 Pseudomonas putida for preparing deoxidized violacein and uses thereof
PCT/CN2009/000430 WO2010003304A1 (en) 2008-07-11 2009-04-22 Recombinant bacteria for producing deoxyviolacein and uses thereof

Publications (3)

Publication Number Publication Date
GB201100438D0 GB201100438D0 (en) 2011-02-23
GB2473401A GB2473401A (en) 2011-03-09
GB2473401B true GB2473401B (en) 2012-10-17

Family

ID=41506661

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1100438.9A Expired - Fee Related GB2473401B (en) 2008-07-11 2009-04-22 Recombinant bacteria for producing deoxyviolacein and uses thereof

Country Status (6)

Country Link
US (1) US8778654B2 (en)
JP (1) JP5632370B2 (en)
CN (1) CN102099477A (en)
BR (1) BRPI0910514A2 (en)
GB (1) GB2473401B (en)
WO (1) WO2010003304A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8778654B2 (en) 2008-07-11 2014-07-15 Tsinghua University Recombinant bacteria for producing deoxyviolacein and uses thereof
AT512453B1 (en) * 2010-10-22 2015-05-15 Kemijski Inst Improved synthesis of a biosynthetic product having an assembled composition of the biosynthetic enzymes on a nucleotide sequence motif
KR101602772B1 (en) 2014-03-05 2016-03-14 대한민국 Novel microorganism EP15224 producing violacein
GB2537144B (en) * 2015-04-09 2019-11-13 Glen Hastie Nugent David Method of dyeing fabric using microorganisms
KR102006904B1 (en) * 2017-07-07 2019-08-02 울산과학기술원 Microorganism including genetic modification that increase productivity of deoxyviolacein and method for producing deoxyviolacein using the same
CN110643651B (en) * 2019-09-30 2022-11-04 大连民族大学 A method for promoting the expression of deoxyviologen in Chromobacterium violaceum
KR102370964B1 (en) * 2019-12-20 2022-03-10 씨제이제일제당 주식회사 Microorganism including genetic modification that increases activity of multidrug efflux transport system, and method for preparing tryptophan metabolites using the same
KR102404998B1 (en) * 2019-12-20 2022-06-07 씨제이제일제당 주식회사 Multidrug efflux pump variant, polynucleotide coding the variant, microorganism including the variant, and method for producing violacein or deoxyviolacein using the same
CN111748564B (en) * 2020-07-13 2022-07-22 新乡医学院 Genetically modified purple bacteriocin biosynthesis gene cluster, recombinant expression vector, engineered bacteria and their applications

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002050299A2 (en) * 2000-12-20 2002-06-27 Stiftung Alfred-Wegener-Institut Für Polar- Und Meeresforschung Microbiological method for the biosynthesis of natural blue-violet colorants violacein and desoxyviolacein and the utilization thereof
CN101139654A (en) * 2007-07-26 2008-03-12 曹传东 Method for producing fine iron sand powder by using ferro-sulphur ore as raw material and equipment thereof
CN101319213A (en) * 2007-06-07 2008-12-10 上海市血液中心 Bacterial genome DNA extract and its preparation method and application
CN101368169A (en) * 2008-10-17 2009-02-18 清华大学 Recombinant Pseudomonas putida producing deoxyviolet violacein and its application

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101139564B (en) * 2007-08-08 2010-06-09 清华大学 A Strain of Dunella and Its Application
US8778654B2 (en) 2008-07-11 2014-07-15 Tsinghua University Recombinant bacteria for producing deoxyviolacein and uses thereof
CN101319219B (en) * 2008-07-11 2010-12-01 清华大学 A method for producing deoxyviolet bacterin and its special recombinant bacteria

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002050299A2 (en) * 2000-12-20 2002-06-27 Stiftung Alfred-Wegener-Institut Für Polar- Und Meeresforschung Microbiological method for the biosynthesis of natural blue-violet colorants violacein and desoxyviolacein and the utilization thereof
CN101319213A (en) * 2007-06-07 2008-12-10 上海市血液中心 Bacterial genome DNA extract and its preparation method and application
CN101139654A (en) * 2007-07-26 2008-03-12 曹传东 Method for producing fine iron sand powder by using ferro-sulphur ore as raw material and equipment thereof
CN101368169A (en) * 2008-10-17 2009-02-18 清华大学 Recombinant Pseudomonas putida producing deoxyviolet violacein and its application

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Biochemistry 45 (2006) Balibar et al 'In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum' 15444-15457 *
Chem Industry Engineer Progress; Vol 27, pp 315-321 (2008). Wang et al. "Recent research progress of bacterial violaccin" *
Journal of Molecular Microbiology and Biotechnology 2 (2000) August et al 'Sequence analysis and functional characterization of the violacein biosynthetic pathway from Chromobacterium violaceum' 513-519 *
Org Lett; Vol 3, pp 1981-1984 (2001). Brady et al. "Cloning and heterologous expression of a natural product biosynthetic gene cluster from cDNA" *

Also Published As

Publication number Publication date
US20110183384A1 (en) 2011-07-28
WO2010003304A1 (en) 2010-01-14
JP5632370B2 (en) 2014-11-26
GB201100438D0 (en) 2011-02-23
JP2011527183A (en) 2011-10-27
BRPI0910514A2 (en) 2015-09-22
CN102099477A (en) 2011-06-15
US8778654B2 (en) 2014-07-15
GB2473401A (en) 2011-03-09

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