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GB2332908A - Flax promoter for manipulating gene expression - Google Patents

Flax promoter for manipulating gene expression

Info

Publication number
GB2332908A
GB2332908A GB9908939A GB9908939A GB2332908A GB 2332908 A GB2332908 A GB 2332908A GB 9908939 A GB9908939 A GB 9908939A GB 9908939 A GB9908939 A GB 9908939A GB 2332908 A GB2332908 A GB 2332908A
Authority
GB
United Kingdom
Prior art keywords
seq
flax
genes
promoters
gene expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9908939A
Other versions
GB9908939D0 (en
GB2332908B (en
Inventor
Ravinder Kumar Jain
Roberta Gail Thompson
Gordon Grant Rowland
Alan Gordon Mchughen
Samuel Leonard Mackenzie
David Charles Taylor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Council of Canada
Original Assignee
National Research Council of Canada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research Council of Canada filed Critical National Research Council of Canada
Publication of GB9908939D0 publication Critical patent/GB9908939D0/en
Publication of GB2332908A publication Critical patent/GB2332908A/en
Application granted granted Critical
Publication of GB2332908B publication Critical patent/GB2332908B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C12N15/8226—Stem-specific, e.g. including tubers, beets
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention relates to promoter sequences obtained from flax and useful for modification of flax and other plants for expression of endogenous or foreign genes. The promoters are the SEQ ID NO. 3 and SEQ ID NO. 4 and are obtained from newly illucidated structures of two SAD genes in flax, namely SEQ ID NO. 1 and SEQ ID NO. 2. The promoters have been inserted into cloning plasmids and deposited at the American Type Culture Collection as plasmids pCDC220 and pCDC214 under deposit numbers ATCC 98192 and ATCC 98193, respectively. The promoters may be used in conjunction with genes to modify characteristics of flax and other plants. The invention includes the SAD genes themselves and DNA sequences substantially homologous to SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, and SEQ ID NO. 4, as well as significant parts thereof.
GB9908939A 1996-10-31 1997-10-30 Flax promoter for manipulating gene expression Expired - Fee Related GB2332908B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2941696P 1996-10-31 1996-10-31
PCT/CA1997/000812 WO1998018948A1 (en) 1996-10-31 1997-10-30 Flax promoters for manipulating gene expression

Publications (3)

Publication Number Publication Date
GB9908939D0 GB9908939D0 (en) 1999-06-16
GB2332908A true GB2332908A (en) 1999-07-07
GB2332908B GB2332908B (en) 2001-03-28

Family

ID=21848902

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9908939A Expired - Fee Related GB2332908B (en) 1996-10-31 1997-10-30 Flax promoter for manipulating gene expression

Country Status (4)

Country Link
AU (1) AU4858897A (en)
CA (1) CA2268745A1 (en)
GB (1) GB2332908B (en)
WO (1) WO1998018948A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE326539T1 (en) * 1999-08-27 2006-06-15 Sembiosys Genetics Inc SEED-SPECIFIC PROMOTER FROM FLAX (LINUM USITATISSIMUM)
US7642346B2 (en) * 1999-08-27 2010-01-05 Sembiosys Genetics Inc. Flax seed specific promoters
US6777591B1 (en) * 1999-08-27 2004-08-17 Sembiosys Genetics Inc. Legume-like storage protein promoter isolated from flax and methods of expressing proteins in plant seeds using the promoter
EP1925671A1 (en) * 2001-06-06 2008-05-28 Bioriginal Food & Science Corp. Flax (Linum usitatissimum L.) seed-specific promoters
ES2312620T3 (en) 2001-06-06 2009-03-01 BIORIGINAL FOOD & SCIENCE CORP. SPECIFIC SEQUENCES OF LINEN SEED (LINUM USITATISSIUMUM L.).
AU2006212647A1 (en) * 2005-02-09 2006-08-17 Bioriginal Food & Science Corp. Novel omega-3 fatty acid desaturase family members and uses thereof
US9745362B2 (en) 2009-12-09 2017-08-29 Shengjun An Seed-specific expression vector and its construction methods and applications

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991013972A1 (en) * 1990-03-16 1991-09-19 Calgene, Inc. Plant desaturases - compositions and uses
WO1991018985A1 (en) * 1990-05-25 1991-12-12 E.I. Du Pont De Nemours And Company Nucleotide sequence of soybean stearoyl-acp desaturase gene
NL9002130A (en) * 1990-09-28 1992-04-16 Stichting Tech Wetenschapp Cruciferous stearoyl-ACP-desaturase coding sequences - for modifying lipid biosynthesis in plants, esp. oilseed rape
EP0561569A2 (en) * 1992-03-13 1993-09-22 The Lubrizol Corporation Modification of vegetable oils using desaturase
WO1995007357A2 (en) * 1993-09-04 1995-03-16 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Promoters
EP0736598A1 (en) * 1993-12-28 1996-10-09 Kirin Beer Kabushiki Kaisha Gene for fatty acid desaturase, vector containing said gene, plant containing said gene transferred thereinto, and process for creating said plant

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5475099A (en) * 1990-08-15 1995-12-12 Calgene Inc. Plant fatty acid synthases

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991013972A1 (en) * 1990-03-16 1991-09-19 Calgene, Inc. Plant desaturases - compositions and uses
WO1991018985A1 (en) * 1990-05-25 1991-12-12 E.I. Du Pont De Nemours And Company Nucleotide sequence of soybean stearoyl-acp desaturase gene
NL9002130A (en) * 1990-09-28 1992-04-16 Stichting Tech Wetenschapp Cruciferous stearoyl-ACP-desaturase coding sequences - for modifying lipid biosynthesis in plants, esp. oilseed rape
EP0561569A2 (en) * 1992-03-13 1993-09-22 The Lubrizol Corporation Modification of vegetable oils using desaturase
WO1995007357A2 (en) * 1993-09-04 1995-03-16 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Promoters
EP0736598A1 (en) * 1993-12-28 1996-10-09 Kirin Beer Kabushiki Kaisha Gene for fatty acid desaturase, vector containing said gene, plant containing said gene transferred thereinto, and process for creating said plant

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CSIRO, Div. of Plant Ind., Report 1985-86 (1986) pp.12-16 - A.Green et al. *
EMBL Sequence Database (26/2/93) Acc.No.X70962 - S.Singh et al. *
Euphytica 1995 85 (1-3) pp.317-321 - G.Rowland et al. *
Plant Physiology 1992 100 pp.894-901 - J.Polashock et al. *
Plant Physiology 1994 (104) p.1075 - S.Singh et al. *
Proc.Natl.Acad.Sci.USA 1992 89 pp.2624-2628 - D.Knutzon et al. *

Also Published As

Publication number Publication date
GB9908939D0 (en) 1999-06-16
AU4858897A (en) 1998-05-22
GB2332908B (en) 2001-03-28
CA2268745A1 (en) 1998-05-07
WO1998018948A1 (en) 1998-05-07

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Legal Events

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PCNP Patent ceased through non-payment of renewal fee

Effective date: 20031030