GB2392093A - Pharmaceutical formulations - Google Patents

Abstract

The invention relates to pharmaceutical formulations, and more particularly to formulations containing cannabinoids for administration via a pump action spray. In particular, the invention relates to pharmaceutical formulations, for use in administration of lipophilic medicaments via mucosal surfaces, comprising: at least one lipophilic medicament, a solvent and a co-solvent, wherein the total amount of solvent and co-solvent present in the formulation is greater than 55% wt/wt of the formulation and the formulation is absent of a self emulsifying agent and/or a fluorinated propellant.

Description

- - 1 Pharmaceutical Formulations Fleld Of The Invention The present

Invention relates to pharmaceutical 5 formulations, and more particularly to formulations containing cannabinoids for administration via a pump action spray.

Sack round Of The Inventlon It has long been known to introduce drugs into the 10 systemic circulation system via a contiguous mucous membrane to increase onset of activity, potency etc. For example, US 3,560,625 disclose aerosol formulations for introducing an alkoxybenzamide into the systemic circulatory 15 system. Two different types of aerosol formulations are disclosed: a) fluorinated hydrocarbon type comprising 2% by weight alkoxybenzamide, 18% ethanol, and 80% propellant; and b) nebuliser type comprising 0.5% by weight alkoxybenzamide, 20 a mixed solvent system comprising 10.3 % ethanol and 31.4% propylene glycol and 57.8% deionised water.

US 3,560,625 identifies a problem in finding a suitable solvent system to produce an aerosol spray for inhalation of the 25 ortho-ethoxybenzamide, due to the fact that whilst ethanol was undoubtedly the best solvent, a mixture containing more than 18% of ethanol by weight produced an unpleasant oral reaction which more than counterbalanced the efficacy of the oral route.

30 When the present applicant set out to produce spray formulations for a botanical drug substance comprising one or more cannabinoids they were aware that the highly lipophylic nature of the cannabinoids could present problems in formulating the active component(s).

The present applicant first sought to develop a formulation for oromucosal, preferably sublingual, delivery in a pressurized aerosol or spray form, as disclosed lo international patent application PCT/GBO1/01027. Their initial focus was on 40 propellant driven systems with HFC-123a and HFC-227 but these

2 - proved to be unsuitable as solvents for the cannablnoids. The formulations comprised synthetic A9-THC in amounts from 0.169 to 0.% wt/wt, with ethanol as the primary solvent in amounts up to 20.51\ by weight. One particular composition comprised 0.164% 5 synthetic A9-TlIC, 4.992% ethanol, 9.992% propylene glycol and 89.582! pl34a (propellant).

The applicant found that even at ethanol levels of 20% by volume of the total formulation volume they were unable to 10 dissolve sufficient levels of A9-THC in a standard spray dose to meet clinical needs, because of the cannabinoids poor solubility in the propellant. They also found that the ethanol level could not be increased, as the delivery characteristics of the device nozzle altered substantially when the lower volatility solvents 15 were increased above a critical ratio. The HFC- 123a and HFC-227 propellant sprays delivered a maximum of 7 mg/ml, whereas initial clinical studies suggested the formulations would be required to contain up to 50 mg cannabinoids/ml.

20 Thus, the present applicants focussed on self-emulsifying drug delivery systems, as are discussed in detail in a review article European Journal of Pharmaceutics and Blopharmaceutics 50 (2000) 179-188, which concluded that the poor aqueous solubllity of many chemical entitles represents a real challenge 25 for the design of appropriate formulations aimed at enhancing oral bloevailabillty.

In their co-pending International application PCT/GB02/006Z0 the applicant discloses a wide range of 30 cannabinoid-containing formulations containing at least one self-emulsifying agent. The inclusion of at least one self emulslfying agent was thought necessary to get the formulation to adhere to the mucosal surface in order to achieve sufficient absorption of the cannabinoids. One particular formulation 35 comprised 2S, by at glycerol mono-oleate, 5% COME of G1 cannabis to give THC, 5% COME of G5 cannabis to give CBD, 44% ethanol BP and 44% propylene glycol.

Summary Of The Invention

40 Surprlalogly, the applicant has found that they do not

- 3 - absolutely require the presence of a self-emulsifying agent in a liquid formulation to achieve a satisfactory dosage level by; oromucosal, and specifically sub-lingual or buccal, application.

5 Indeed, contrary to the teachings of US 3,560,625 and the European Journal of Pharmaceutics and Biopharmaceutics 50 (2000) 179-188, they have been able to produce a simple and effective vehicle for delivering a lipophilic medicament in a liquid spray. According to a specific aspect of the present invention J there is provided a pharmaceutical formulation consisting essentially of one or more cannabinoids, ethanol and propylene glycol. Preferably the one or more cannabinoids are present in the form of at least one extract from at least one cannabis plant.

The cannabis plant(s) preferably include at least one cannabis chemovar. Most preferably the plant extract will be a botanical 20 drug substance (BDS), as defined herein.

Optionally, the formulation may additionally contain a flavour, such as, for example, peppermint oil.

25 The formulation may also contain, in addition to the cannabinold(s), a further active agent, which is preferably an opiate, for example morphine. Thus, it is contemplated to provide a formulation consisting essentially of one or more cannabinoids, ethanol, propylene glycol and an opiate, 30 preferably morphine.

A typical liquid pharmaceutical formulation according to this specific aspect of the invention, given by way of example and not intended to be limiting to the invention, may contain in 35 a 1 ml vol: THC 25-50 mg/ml, preferably 25 mg/ml (based on amount of cannabinoid in a botanical drug substance), CBD 25-50 mg/ml, preferably 25 mg/ml (based on amount of cannabinoid in a botanical drug substance), propylene glycol 0.5 ml/ml, peppermint oil 0.0005 ml/ml, and ethanol (anhydrous) qs to 1 ml.

4 - Other preferred formulations include a "high THC" formulation comprising in a 1 ml vol: THC 25 mg/ml (based on amount of cannablnold In a botanical drug substance), propylene glycol 0.5 ml/ml, peppermint oil 0. 0005 ml/ml, and ethanol 5 (anhydrous) qs to 1 ml; and a high CBD" formulation comprising In a 1 ml vol: COD 25 mg/ml (based on amount of cannabinoid in a botanical drug substance), propylene glycol 0.5 ml/ml, peppermint oil 0.0005 ml/ml, and ethanol (anhydrous) qs to 1 ml.

10 In these formulations the cannbinoids are added as botanical drug substances derived from cannabis plants, quoted amounts of cannabinoids correspond to total amount (weight) of cannabinoid present in lml of the final formulation. The skilled reader will appreciate that the total amount of BDS 15 which must be added in order to achieve the desired amount of cannabinoid in the final formulation will be dependent on the concentration of cannabinoid present in the BDS, which will vary between different batches of BDS.

20 The finding that such a simple combination of one or more cannabinolds, ethanol and propylene glycol can be used effectively In a pump action spray was unexpected.

The applicant has found that, where the solvent/co-solvent 25 system Is ethanol/propylene glycol and the lipophilic medicament comprises one or more cannabinoids in the form of a botanical drug substance (BDS), the limits in which the solvent/co-solvent will work effectively are quite narrow, as discussed below.

30 More broadly speaking, and according to a general aspect of the invention, there is provided a liquid pharmaceutical formulation, for use in administration of a lipophilic medlcament via a mucosal surface, comprising at least one lipophlllc medicament, a solvent and a co-solvent, wherein the 35 total amount of solvent and co-solvent present in the formulation is greater than 55% wt/wt of the formulation and the formulation is absent of a self-emulsifying agent and/or a fluorinated propellant.

- 5 Preferably the amount of solvent/co-solvent is greater than 80%, more preferably in the order 90-98.

Preferably the formulation has a water content of less 5 than 5%.

Preferably the formulation does not contain any type of propellant. 10 The formulation also lacks any self-emulsifying agent.

Self-emulalfying agents are defined herein as an agent which wiLl form an emulsion when presented with an alternate phase with a minimum energy requirement. In contrast, an emulsifying agent, as opposed to a selfemulsltying agent, is one requiring 15 additional energy to form an emulsion. Generally a self emulsifying agent will be a soluble soap, a salt or a sulphated alcohol, especially a non-ionic surfactant or a quaternary compound. Exemplary self-emulsifying agents include, but are not limited to, glyceryl mono oleate (esp. SE grade), glyceryl 20 monostearate (esp. SE grade), macrogols, and polyoxyhydrogenated castor oils e.g. cremophor.

The formulation may additionally comprise a flavouring.

The preferred flavourlng is peppermint oil, preferably in an 2S amount by vol of up to 0.1%, typically 0.05% v/v.

Preferably the solvent IS selected from Cl-C4 alcohols.

The preferred solvent is ethanol.

30 Preferably the co-solvent is a solvent which allows a lower amount of the "primary" solvent to be used. In combination with the "primary" solvent it should solubilise the lipophylic medicament sufficiently that a medically useful amount of the lipophylic medicament is solubilised. A medically 35 useful amount will vary with the medicament, but for cannabinoids will be an amount of at least 1.0 mg/O.l ml of solvent/cosolvent Preferred co-solvents are selected from glycols, sugar 40 alcohols, carbonate esters and chlorinated hydrocarbons.

- 6 - The glycols are preferably selected from propylene glycol and glycerol, with propylene glycol being most preferred. The carbonate ester is preferably propylene carbonate.

s The most preferred combination is ethanol as the solvent and propylene glycol as the co-solvent.

The preparation of liquid formulations for oropharangeal 10 delivery of cannablnoids poses a number of problems. First, it is necessary to deliver at least 1.0 mg, more preferably at -

least 2.5 mg and even more preferably at least 5 mg of 3 cannablnolds per 0.1 ml of liquid formulation to achieve a therapeutic effect In a unit dose. In this regard a patient may 15 require up to 120 mg cannabinoid/day, on average around to rng/day to be taken in a maximum of six doses.

In the case of a sublingual or buccal delivery, this means delivering this quantity of the active ingredient in an amount 20 of formulation which will not be swallowed by the patient, if the active ingredient is to be absorbed transmucosally.

Whilst such amounts can be achieved by dissolving the cannabinoid in ethanol as the solvent, high concentrations of 25 ethanol provoke a stinging sensation and are beyond the limit of tolerability. There IS thus a need to use a co-solvent in order to reduce the amount of ethanol, whilst still enabling sufficient 30 quantities of cannabinoid to be solubilised.

The applicant has discovered that the choice of co-solvent is limited. Preferred co-solvents should have a solubilizing effect sufficient to allow enough cannabinoid to be solubilised -

35 in a unit dose, namely at least 1.0 mg/0.1 ml of formulation, and which allows the amount of solvent present to be reduced to a level which is within the limits of patient tolerability.

Particularly suitable co-solvents which fulfill these criteria are propylene glycol and glycerol.

In a preferred embodiment the total amount of solvent and co-solvent present in the formulation, is greater than 65% w/w, more preferably greater than 70% w/w, more preferably greater than 75% w/w, more preferably greater than 80% w/w, more 5 preferably greater than 85% w/w of the formulation. Most preferably tile total amount of solvent and cosolvent present in the formulation is in the range from 80% w/w to 98% w/w of the formulation. 10 In a preferred embodiment the formulations according to the invention are liquid formulation administered via a pump action spray. Pump-action sprays are characterized in requiring the application of external pressure for actuation, for example external manual, mechanical or electrically initiated pressure.

15 This is in contrast to pressurized systems, e.g. propellant driven aerosol sprays, where actuation IS typically achieved by controlled release of pressure e.g. by controlled opening of a valve. 20 Pump-actlon sprays are found to be particularly beneficial when lt comes to delivering cannablnolds. Indeed, previously people have focussed their attention on solvent systems including a propellant.

25 Whilst it has been recognized that there are disadvantages with such systems, including the speed of delivery, those skilled in the art have tried to address this by slowing the propellant or by altering the nozzle. The applicants have found that by using a pump spray with their formulations they are able 30 to produce a spray in which the particles have a mean aerodynamic particle size of between 15 and 45 microns, more particularly between 20 and 40 microns and an average of about 33 microns. These contrast with particles having a mean aerodynamic particle size of between 5 and lo microns when 35 delivered us log a pressurized system.

In fact, comparative tests by the applicant have shown such a pump-action spray system to have advantages in being able to deliver the active components to a larger surface area within 40 the target area. This is illustrated with reference to the

- 8 - accompanying Example 3.

The variation in particle distribution and sprayed area has been demonstrated by direct experiment. A formulation as 5 described in the accompanying Example 4 was filled into a pump action spray assembly (Valois vial type VP7100 actuated). The same formulation was filled into a pressurized container powered by HFA 134a.

10 Both containers were discharged at a distance of 50 mm from a sheet of thin paper held at right angles to the direction of travel of the jet. The pattern of spray produced in both cases by discharge of 100 Al was then visualised against the light. In both cases the pattern of discharge was circular and 15 measurements were as follows: Mean Diameter (mm) Mean Area (A 2) Pump Action Spray 23 425.5 Pressurised Spray 16 201.1 The pressurized spray produced pooling of liquid at the centre of the area. The pump action spray gave a more even 20 spray pattern and less "bounce back". There was also a significantly greater area covered by the pump action spray.

The conditions under which this test was carried out are relevant to the in-practice use of the device. A wider area of j buccal mucosa can be reached by the pump action spray compared j 25 with the pressurized spray.

For pump spray applications the solvent/co-solvent combination must have a viscosity within the viscosity range defined by the preferred solventicosolvent combination. Thus 30 it should be a viscosity ranging between that for an ethanol/propylene glycol combination where the ethanol/propylene glycol are present in the relative proportions by vol of 60/40 and 90/60, more preferably still 55/45 to 45/55 and most preferably about 50/SO.

The VlsCOSlty of the resulting formulation when packaged for delivery by pump action through a mechanical pump such as,

- 9 - for example, a VP7 actuator valve (Valois), allows the resulting aerosol to deliver a spray having a mean aerodynamic particle size of from 20-40 microns, more preferably 25-35 and most preferably with an average particle size of from 30-35 microns.

5 Thls maxlmises contact with the target mucosal membrane for subllngual/buccal delivery.

Preferably the formulations according to the general and specific aspects of the invention comprises as the lipophilic 10 medicament one or more cannabinoids.

Preferably the lipophilic medicament is at least one extract from at least one cannabis plant. The cannabis plants) preferably include at least one cannabis chemovar. Most 15 preferably the plant extract will be a botanical drug substance (BDS), as defined herein.

A "plant extract" is an extract from a plant material as defined in the Guidance for Industry Botanical Drug Products 20 Draft Guidance, August 2000, US Department of Health and Human Services, Food and Drug Administration Centre for Drug Evaluation and Research.

"Plant material" is defined as a plant or plant part (e.g. 25 bark, wood, leaves, stems, roots, flowers, fruits, seeds, berries or parts thereof) as well as exudates.

The term "Cannabis plants)" encompasses wild type j Cannabls satlva and also variants thereof, including cannabis 30 chemovars which naturally contain different amounts of the individual cannabinoids, Cannabis sativa subspecies indict including the variants var. indict and var. kafiristanica, Cannabis indica and also plants which are the result of genetic crosses, self-crosses or hybrids thereof. The term "Cannabis 35 plant material" is to be interpreted accordingly as encompassing plant material derived from one or more cannabis plants. For the avoidance of doubt it is hereby stated that "cannabis plant material" includes dried cannabis biomass.

40 In the context of this application the terms "cannabis

- 10 extract" or "extract from a cannabis plant", which are used interchangeably, encompass "Botanical Drug Substances" derived from cannabis plant material. A Botanical Drug Substance is defined in the Guldance for Industry Botanical Drug Products 5 Draft Guidance, August 2000, US Department of Health and Human Services, Food and Drug Administration Centre for Drug Evaluation and Research as: "A drug substance derived from one or more plants, algae, or macroscopic fungi. It is prepared from botanical raw materials by one or more of the following 10 processes: pulverization, decoction, expression, aqueous extraction, ethanolic extraction, or other similar processes."

A botanical drug substance does not include a highly purified or chemically modified substance derived from natural sources.

Thus, in the case of cannabis, "botanical drug substances" 15 derived from cannabis plants do not include highly purified, Pharmacopoeial grade cannabinoids.

Cannabis based medicine extracts (CBMEs)", such as the CBMEs prepared using processes described in the accompanying 20 examples, are classified as "botanical drug substances", according to the deflnlUlon given in the Guidance for Industry Botanical Drug Products Draft Guidance, August 2000, US Department of Health and Human Services, Food and Drug Administration Cent re for Drug Evaluation and Research.

"Botanical drug substances" derived from cannabis plants include primary extracts prepared by such processes as, for example, maceration, percolation, extraction with solvents such as C1 to C5 alcohols (e.g. ethanol), Norflurane (HEA134a), 30 HEA227 and liquid carbon dioxide under sub-critical or super crlUlcal conditions. The primary extract may be further purified for example by super-critical or sub-critical solvent extraction, vaporization or chromatography. When solvents such as those listed above are used, the resultant extract contains 35 non-specific lipid-soluble material. This can be removed by a variety of processes including "winterisation", which involves chilling to -20 C followed by filtration to remove waxy ballast, extraction with liquid carbon dioxide and by distillation.

40 In the case where the cannabinoids are provided as a BDS,

l - 11 -

the BDS is preferably obtained by CO2 extraction, under sub crltical or super-critical conditions, followed by a secondary extraction, e.g. an ethanolic precipitation, to remove a substantial proportion of waxes and other ballast. This is 5 because the ballast includes wax esters and glycerides, unsatutrated fatty acid residues, terpenes, carotenes, and flavenolds which are not very soluble in the chosen solvent/co solvent, particularly the preferred co-solvent, propylene glycol, and will precipitate out. Most preferably the BDS is 10 produced by a process comprising decarboxylation, extraction with liquid carbon dioxide and then a further extraction to remove significant amounts of ballast. Most preferably the ballast is substantially removed by an ethanolic precipitation.

15 Most preferably, cannabis plant material is heated to a defined temperature for a defined period of time in order to decarboxylate cannabinoid acids to free cannabinoids prior to extraction of the BDS.

20 Preferred "botanical drug substances" include those which are obtainable by using any of the methods or processes specifically disclosed herein for preparing extracts from cannabis plant material. The extracts are preferably substantially free of waxes and other non- specific lipid soluble 25 material but preferably contain substantially all of the cannabinoids naturally present in the plant, most preferably in substantially the same ratios in which they occur in the intact cannabis plant.

30 Botanical drug substances are formulated into "Botanical Drug Products" which are defined in the Guidance for Industry Botanical Drug Products Draft Guidance, August 2000, US Department of Health and Human Services, Food and Drug Administration Centre for Drug Evaluation and Research as: "A 35 botanical product that is intended for use as a drug; a drug product that is prepared from a botanical drug substance."

"Cannabis plants" includes wild type Cannabis saliva and variants thereof, including cannabis chemovars which naturally 40 contain different amounts of the individual cannabinoids.

- 12 The term "cannabinoids" also encompasses highly purified, Pharmacopoeial Grade substances, which may be obtained by purification from a natural source or via synthetic means.

S Thus, the formulations according to the invention may be used for delivery of extracts of cannabis plants and also individual cannabinoids, or synthetic analogues thereof, whether or not -

derived from cannabis plants, and also combinations of cannabinoids. Preferred cannabloods include, but are not limited to, -

tetrahydrocannabinoids, their precursors, alkyl (particularly propyl) analogues, cannabidiols, their precursors, alkyl (particularly propyl) analogies, and cannabinol. In a preferred 15 embodiment the formulations may comprise any cannabinoids selected from tetrabydrocannabinol, A9tetrahydrocannabinol (THC), A8-tetrahydrocannabinol, A9tetrahydrocannabinol propyl analogue (THCV), cannabidiol (CBD), cannabidiol propyl analogue (CBDV), cannabinol, cannabichromene, cannabichromene propyl 20 analogue and cannabigerol, or any combination of two or more of these cannabinoids. THCV and CBDV (propyl analogies of THC and CBD, respectively) are known cannabinoids which are predominantly expressed in particular Cannabis plant varieties and it has been found that THCV has qualitative advantageous 25 properties compared with THC and CBD respectively. Subjects taking THCV report that the mood enhancement produced by THCV is less disturbing than that produced by THC. It also produces a less severe hangover.

30 Most preferably the formulations will contain THC and/or CBD. In a preferred embodiment the formulations may contain specific, pre-defined ratios by weight of different cannbinoids, 35 e.g. specific ratios of CBD to THC, or tetrahydrocannabinovarin (THCV) to cannabidivarin (CBDV), or THCV to THC. Certain specific ratios of cannabinoids have been found to be clinically useful in the treatment or management of specific diseases or medical conditions. In particular, certain of such formulations 40 have been found to be particularly useful in the field of pain

- 13 relief and appetite stimulation.

It has particularly been observed by the present applicants that combinations of specific cannabinoids are more 5 beneficial than any one of the individual cannabinoids alone.

Preferred embodiments are those formulations in which the amount of CBD is in a greater amount by weight than the amount of THC.

Such formulations are designated as "reverse-ratio" formulations and are novel and unusual since, in the various varieties of lo medicinal and recreational Cannabis plant available world-wide, CBD is the minor cannabinoid component compared to THC. In other embodiments THC and CBD or THCV and CBDV are present in approximately equal amounts or THC or THCV are the major component and may be up to 95.S! of the total cannabinoids IS present. Preferred formulations contain THC and CBD in defined ratios by weight. The most preferred formulations contain THC and CBD in a ratio by weight in the range from 0.9:1.1 to 20 1.1:0.9 THC:CBD, even more preferably the THC:CBD ratio is substantially 1:1. Other preferred formulations contain the following ratios by weight of THC and CBD:greater than or equal to 19:1 THC:CBD, greater than or equal to 19:1 CBD:THC, 4.5:1 THC:CBD, l:4 THC:CBD and 1:2.7 THC:CBD. For formulations 25 wherein the THC:CBD ratio is substantially 1:1 it is preferred that the formulation includes about 2.5 g/ml of each of THC and CBD. Cannabis has been used medicinally for many years, and in 30 Victorlan times was a widely used component of prescription medicines. It was used as a hypnotic sedative for the treatment of "hysteria, delirium, epilepsy, nervous insomnia, migraine, pain and dysmenorrhosa". The use of cannabis continued until the middle of the twentieth century, and its usefulness as a 35 prescription medicine is now being re- evaluated. The discovery of specific cannabinoid receptors and new methods of administration have made it possible to extend the use of cannabis-based medicines to historic and novel indications.

40 The recreational use of cannabis prompted legislation

- 14 which resulted in the prohibition of its use. Historically, cannabis was regarded by many physicians as unique; having the ability to counteract pain resistant to opioid analgesics, in conditions such as spinal cord injury, and other forms of 5 neuropathic pain including pain and spasm in multiple sclerosis.

In the United States and Caribbean, cannabis grown for recreational use has been selected so that it contains a high content of tetrahydrocannabinol (THC), at the expense of other Lo cannabinolds. In the Merck Index (1996) other cannabinoids known to occur in cannabis such as cannabidiol and cannabinol were regarded as inactive substances. Although cannabidiol was formerly regarded as an inactive constituent there is emerging evidence that it has pharmacological activity, which is 15 different from that of THC in several respects. The therapeutic effects of cannabis cannot be satisfactorily explained just in terms of one or the other "active" constituents.

It has been shown that tetrahydrocannabinol (THC) alone 20 produces a lower degree of pain relief than the same quantity of THC given as an extract of cannabis. The pharmacological basis underlying this phenomenon has been investigated. In some cases, THC and cannabidiol (CBD) have pharmacological properties of opposite effect In the same preclinical tests, and the same 25 effect in others. For example, in some clinical studies and from anecdotal reports there is a perception that CBD modifies the psychoactive effects of THC. This spectrum of activity of the two cannablnoids may help to explain some of the therapeutic benefits of cannabis grown in different regions of the world.

30 It also points to useful effects arising from combinations of THC and CBD. These have been investigated by the applicant.

Table 1 below shows the difference in pharmacological properties of the two cannabinoids.

- 15 Table 1

Effect THC TRCV CBD CDDV Reference CB1 (Brain receptors) ++ + Pertwee et al, 1998 CB2 (Peripheral receptors) + -

CNS [Effects Antlconvulsant t -- ++ Carlini et al, 1973 Antlmetrazol - GW Data Antl-electroshock - ++ GW data Muscle Relaxant -- ++ Petro, 1980 Antlnoclceptlve ++ + GW data Catalepsy ++ ++ GW data Psychoactive ++ - GW data Antipsychotic - ++ Zuardi et al, 1991 Neuroprotectlve antioxidant + + + Hampson A J et al, activity* ++ - 1998 Antlemetlc + + Sedatlon (reduced spontaneous activity) ++ Zuardi et al, 1991 Appetlte stimulation ++ Appetlte suppression - ++ Anxlolytlc GW data Cardiovascular EffectsBradycardia - + Smiley et al, 1976 Tachycardia + -

Hypertension + -

Hypotens1on - + Adams et al, 1977 Anti-inflammatory _ + Brown, 1998 Immunomodulatory/anti-inflammatory activity Raw Paw Oedema Test - ++ GW data Cox 1 GW data Cox 2 GW data TNFa Antagonism + + ++ ++ Glaucoma ++ + i 5 Effect is CB1 receptor independent.

t THC is pro convulsant THC has a biphasic effect on blood pressure; in naive patients it may produce postural hypotension and it has also been reported to produce hypertension on prolonged usage. GW 10 Internal Report No 002/000159.

From these pharmacological characteristics and from direct experiments carried out by the applicant it has been shown, surprisingly, that combinations of THC and CBD in varying 15 proportions are particularly useful in the treatment of certain

- 16 -

therapeutic conditions. It has further been found clinically that the toxicity of a mixture of THC and CBD iS less than that of THC alone.

5 Accordingly, the invention provides pharmaceutical formulations, having all the essential features described above, which comprise cannabinoids as the active agents and which have specific ratios of CBD to THC, which have been found to be clinically useful in the treatment or management of specific 10 diseases or medical conditions.

In a further aspect the invention also relates to pharmaceutical formulations having all the essential features defined above, and which have specific ratios of 15 tetrabydrocannabinovarin (THCV) or cannabidivarin (CBDV). THCV and CBDV (propyl analogies of THC and CBD, respectively) are known cannabinolds which are predominantly expressed in particular Cannabis plant varieties and it has been found that THCV has qualitative advantageous properties compared with THC 20 and CBD respectively. Subjects taking THCV report that the mood enhancement produced by THCV IS less disturbing than that produced by THC. It also produces a less severe hangover.

The invention still further relates to pharmaceutical 25 formulations, having all the essential features as defined above, which have specific ratios of THCV to THC. Such formulations have been found to be particularly useful in the field of pain relief and appetite stimulation.

30 It has particularly been observed by the present applicants that the combinations of the specific cannabinoids are more beneficial than any one of the individual cannabinoids alone. Preferred embodiments are those formulations in which the amount of CBD is In a greater amount by weight than the amount 35 of THC. Such formulations are designated as "reverseratio" formulations and are novel and unusual since, in the various varieties of medicinal and recreational Cannabis plant available worldwide, CBD is the minor cannabinoid component compared to THC. In other embodiments THC and CBD or THCV and CBDV are 40 present in approximately equal amounts or THC or THCV are the

- 17 major component and may be up to 95.5% of the total cannabinoids present. Particularly preferred ratios of cannabinolds and the 5 target medical conditions for which they are suitable are shown lo Table 2 below. Other preferred ratios of THC:CBD, THCV:C8DV and THC:TCHV and preferred therapeutic uses of such formulations are set out in the accompanying claims.

Table 2: Target Therapeutic Groups for Different Ratios of Cannabinold 15 Product group Ratio THC:CBD Target Therapeutic Area High THC > 95:5 Cancer pain, migraine, appetite stimulation Even ratio 50:50 Multiple sclerosis, spinal cord injury, peripheral neuropathy, other neurogenic pain. Reverse/Broad ratio COD <25:75 Rheumatoid arthritis, Inflammatory bowel diseases.

High COD <5:95 Psychotic disorders 30 (schizophrenia), Epilepsy & movement disorders Stroke, head injury, Disease modification in RA and other inflammatory 35 conditions Appetite suppression Formulations containing specific, defined ratios of cannabinoids may be formulated from pure cannabinoids in 40 combination with pharmaceutical carriers and excipients which

- 18 are well-known to those skilled in the art. Pharmaceutical grade "pure" cannabinoids may be purchased from commercial suppliers, for example CBD and THC can be purchased from Slgma-Aldrich Company Ltd. Fancy Road, Poole Dorset, BH12 4QH, 5 or may be chemically synthesized. Alternatively, cannabinoids may be extracted from Cannabis plants using techniques well-known to those skilled in the art.

In preferred embodiments of the invention the formulations 10 comprise extracts of one or more varieties of whole Cannabis plants, particularly Cannabis saliva, Cannabis indica or plants which are the result of genetic crosses, self-crosses or hybrids thereof. The precise cannabinoid content of any particular cannabis variety may be qualitatively and quantitatively 15 determined using methods well known to those skilled in the art, such as TLC or HPLC. Thus, one may chose a Cannabis variety from which to prepare an extract which will produce the desired ratio of COD to THC or CBDV to THCV or THCV to THC.

Alternatively, extracts from two of more different varieties may 20 be mixed or blended to produce a material with the preferred cannabinoid ratio for formulating into a pharmaceutical formulation. The preparation of convenient ratios of THC- and 25 CBD-containing medicines is made possible by the cultivation of specific chemovars of cannabis. These chemovars (plants distinguished by the cannabinoids produced, rather than the morphological characteristics of the plant) can be been bred by a variety of plant breeding techniques which will be familiar to 30 a person skilled in the art. Propagation of the plants by cuttings for production material ensures that the genotype is fixed and that each crop of plants contains the cannabinoids in substantially the same ratio.

35 Furthermore, it has been found that by a process of horticultural selection, other chemovars expressing their cannabinoid content as predominantly tetrahydrocannabinovarin (THCV) or cannabidivarin (CBDV) can also be achieved.

40 Horticulturally, it is convenient to grow chemovars

- 19 producing THC, THCV, COD and CBDV as the predominant cannabinoid from cuttings. This ensures that the genotype in each crop is identical and the qualitative formulation (the proportion of each cannabinold in the biomass) IS the same. From these S chemovars, extracts can be prepared by the similar method of extraction. Convenient methods of preparing primary extracts include maceration, percolation, extraction with solvents such as C1 to C5 alcohols (ethanol), Norflurane (HFA134a), HFA227 and liquid carbon dioxide under pressure. The primary extract may 10 be further purified for example by supercritical or subcritical extraction, vaporlsation and chromatography. When solvents such I as those listed above are used, the resultant extract contains non-specific lipid-soluble material or "ballast". This can be removed by a variety of processes including chilling to -ARC 15 followed by filtration to remove waxy ballast, extraction with liquid carbon dioxide and by distillation. Preferred plant cultivation and extract preparation methods are shown in the Examples. The resulting extract IS suitable for incorporation into pharmaceutical preparations.

I I 20

There are a number of therapeutic conditions which may be treated effectively by cannabis, including, for example, cancer pain, migraine, appetite stimulation, multiple sclerosis, spinal cord injury, peripheral neuropathy, other neurogenic pain, 25 rheumatoid arthritis, inflammatory bowel diseases, psychotic disorders (schizophrenia), epilepsy & movement disorders, stroke, head injury, appetite suppression. The proportion of different cannabinoids in a given formulation determines the ! specific therapeutic conditions which are best treated (as 30 summarised in Table 2, and stated in the accompanying claims).

The principles of formulation suitable for administration of cannabis extracts and cannabinoids can also be applied to other medicaments such as alkaloids, bases and acids. The 35 requirements are that, if the medicament is insoluble in saliva, it should be solubilised and/or brought into the appropriate unionised form by addition of buffering salts and pH adjustment.

Other lipophilic medicaments which may be included in the 40 general formulations of the invention may include, but are not i

- 20 limited to, morphine, pethidine, codeine, methadone, dlamorphine, fentanyl, alfentanil, buprenorphine, temazepam, lipophilic analgesics and drugs of abuse. The term "drugs of abuse" encompasses compounds which may produce dependence in a 5 human subject, typically such compounds will be analgesics, usually opiates or synthetic derivatives thereof.

The formulation is preferably preferably packaged in a glass vial. It is preferably filled to a slight over-pressure 10 in an inert atmosphere e.g. nitrogen to prevent/slow oxidative breakdown of the cannabinoids, and is contained in a form such that ingress of light is prevented, thereby preventing photochemical degradation of the cannabinoids. This is most effectively achieved using an amber vial, since the applicant IS has determined that it is UV and light in the blue spectrum, typically in the wavelength range 200-500nm, that is responsible for photodegradation.

The invention will be further described, by way of example 20 only, with reference to the following experimental data and exemplary formulations, together with the accompanying Figures, in which: Figure la and lb illustrate mean plasma concentrations of 25 cannabinoids CBD, THC and 11hydroxy THC following administration of high CBD (Fig, la) and high THC (Fig.lb) cannabis extracts to human subjects.

Figure 2 illustrate mean plasma concentrations of cannabinoids 30 CBD, THC and 11-hydroxy THC following administration of a -

cannabis extract containing a 1:1 ratio of THC:CBD to a human subject. Figure 3 illustrates cross-sectional area of aerosol plume vs % 35 propylene glycol in propylene glycol/ethanol liquid spray formulations. Flqure A illustrates viscosity as a function of propylene glycol content in propylene glycol/ethanol liquid spray formulations.

- 21 -

Figure 5 illustrates cross-sectional area of aerosol plume vs viscosity for propylene glycol/ethanol liquid spray formulations. 5 Figures 6 and 6a show results of HPLC analysis of samples drawn from stored, light exposed solutions of THC, before (6) and after (6a) charcoal treatment.

Figures 7 and 7a show results of HPLC analysis of samples drawn 10 from stored, light exposed solutions of CBD, before (7) and after (7a) charcoal treatment.

Development of pump-action spray formulations 15 Initially the applicant looked at cannabinoid uptake in patients by applying drops sublingually (BDS dissolved in a mixture of a glycerol/propylene glycol and ethanol) THC 5mg/ml, CBD 5mg/ml and THC/CBD 5mg/ml plus 5 mg/ml.

20 The results are noted In table 3 below: Table 3

Initial absorption 20 min T max awrox 2 hours . C max 6ng/ml THC, 2 ngJml CBD _ AUC 0-12 approx 1 6ng.h/mlTHC, Sng.h/mlCBD following a dose of approx 20mg of each cannabinoids Plasma levels after 6 hours were about I ng/mi THC and 0.5 ng/ml CBD 25 The proportion of 11 hydroxy tetrabydro cannabinol to THC (AUC 0-12) was about 1.9 indicating a significant amount of oral ingestion may have occurred.

On moving to a pump action sublingual spray (following 30 problems solubllising cannabinoids with hydroflurocabon propellant systems) the applicant obtained the results noted in table 9. The solvent system comprised 50:50 ethanol to propylene glycol (v/v ratio) with THC 25mg/ml; CBD 50 mg/ml and THC/CBD 25mg/ml plus 50 mg/ml respectively.

- 22 Table 4

Initial absor tion 60 min P T max aDDrOx 3 hours C max 6ng/ml THC, 8 ng/ml CBD AUC 0-12 approx tang h/mlTHC, 22ng.h/mlCBD following a dose of approx 21mg ofthc AND 35 mg l CBD Plasma levels after 6 hours were about I ng/ml THC and I ng/ml CBD The proportion of 11 hydroxy tetrahydro cannabinol to THC 5 (AUC 0-12) was about 1.6. The profile for each cannabinoid was similar irrespective of the formulation (THC, CBD, THC plus CBD). After accounting for the different dosages, whilst the 10 extent of absorption was comparable to the drops, the rate of absorption was slower and the proportion metabolized rid Despite the slower rate of absorption the pump spray mechanism and the ethanol/propylene glycol carrier system 15 provided the opportunity to administer sufficient cannabinoids, in a flexible dose form with accuracy and advantageously with reduced metabolism.

The data obtained is illustrated in Figs. la, lb and 2, 20 which show the mean plasma concentrations for the formulations identified with reference to table 3 and 4.

That effective delivery of the cannabinoids can be achieved in a vehicle consisting of ethanol and propylene glycol 25 is illustrated by the plasma levels shown in Figs. la, lb and 2.

These show, respectively, formulations containing the high THC and high CBD formulations in Fig. la and lb. Similarly, the effectiveness of a defined ratio formulation THC:CBD 1:1 is illustrated in Flg.2.

Significantly the ethanol/propylene glycol system was -

found to only work with a pump action spray within quite narrow limits. 35 The findings giving rise to the development of pump spray formulations, as exemplified in formulations 1-4 below, are set

l - 23 out below: Example l-Significance of particle size 5 Applicant observed that the propellant aerosols they were developing suffered from "bounce back" and this appeared to be a function of delivery speed and particle size.

They determined that, in contrast to the propellant driven 10 system, a pump spray could deliver an aerosol plume in which the particle size could be controlled to generate a particle size of between 20 and 40 microns tthus maximizing the amount of material hitting the sublingual/ buccal mucosa and thus the amount of cannabinoids that can be absorbed). To produce 15 particles of the appropriate size the viscosity of the formulation needed to be carefully controlled. If the formulation was too viscous droplet formation was hindered, a jet formed and the valve blocked; If the formulation was not viscous enough they got excessive nebulisation, a plume of broad 20 cross sectional area formed, and the spray was no longer directed solely onto the sublingual/buccal mucosa. This could result in the formulation pooling and some of the formulation being swallowed. In both cases the result is unsatisfactory.

25 In fact, it turned out that for the solvent of preferred choice, ethanol, and the co-solvent of preferred choice, propylene glycol, the working range was fairly narrow as demonstrated below: 30 The viscosity of different combinations of ethanol/propylene glycol were studied and their spray performance with a vp7/lOO valve (Valois) compared. The results are tabulated in table 5 below:

- 24 Table 5

Propylene glycol/ethanol Relative viscosity Spray performance (run time in see) 100/0 442 Jet formed 80/20 160 Jet formed 60/40 80 Some letting 50/50 62 Good aerosol plume 40/60 44 Good aerosol plume 20/80 26 Good aerosol plume 0/100 16 Good aerosol plume 5 From this data it appeared that addition of propylene glycol at greater than 60/40 would not be acceptable. These result, when read alongside US 3, 560,625, could have suggested that the said solvent/co-solvent combination would be no good.

However, applicant found that patients could tolerate ethanol 10 levels of this order when presented in the given formulations.

The effect of viscosity on aerosol plume was quantified by spraying the various formulations at a standard distance of 0.5 cm onto disclosing paper. The distance represents the typical 15 distance between the nozzle of the pump action spray unit and the sub lingual cavity in normal use. The paper was photocopied and the image of the plume excised and weighed to give a relative cross sectional area. The relative value was then converted into a real cross sectional area by dividing this 20 value by the weight per cm2 of the photocopier paper (determined by weighing a known area of paper). The results are given in table 6 below:

Table 6

Propylene glycol/ethanol Area of cross section of spray Dlume 100/0 3. 5cm' - 80/20 14 2cm' 60/40 17 9cm2 50/50 20.7cm 40/60 29.4cm2 20/80 54. 4cm 0/100 93.8cm2 This data is illustrated in Fig 3

- 25 Additionally plots of viscosity of mixtures of ethanol and propylene glycol content Fig. 4 and plume cross section as a function of Viscosity Fig. 5 are given.

The figures emphasise the dramatic and undesirable changes in properties which occur outside the narrow range of ethanol/propylene glycol wt/wt of 60/40 and 40/60, and more particularly still 55/45 to 45/55, most preferably about 50/50.

Other factors are also significant in ensuring the combination is used in a narrow range. Increasing the ethanol levels beyond 60 vol % gives rise to irritation and at propylene glycol levels approaching 60% and as low as 55%, in the case of 15 BDS, non polar derivatives present in the BDS begin to precipitate out on prolonged ambient storage.

Other co-solvents which might be used would be expected to have similar limitations. The more viscous the co-solvent the 20 greater the problem of producing a plume forming spray, and the more polar, the greater the risk that precipitation will be exacerbated. However, because the combination of ethanol/propylene 25 glycol is able to dissolve up to 50 mg/ml (i.e. therapeutically desirable levels of cannabinoids), is non irritating, pharmaceutically acceptable, and the propylene glycol also acts j as a penetration enhancer maximising bioavailability of the ! cannabinoids it is particularly advantageous.

The mean particle size of the preferred compositions have been shown to be 33 pm when tested using a Malvern Marsteriser.

The droplets, which are considerably greater than 5 pm, therefore mlulmise the risk of inhalation of aerosol.

Example 2-Effect of water when the cannabinoids are present in a BDS. The presence of greater than 5% water in the formulation 40 was shown to cause precipitation of the BDS as illustrated by

- 26 the investigation described in Table 7 below: Table 7-Sequential addition of water was made to 5 ml 25mg/ml THC and 5 ml 25mg/ml COD in an ethanol/propylene glycol 5 formulate (50/50).

Vol of water added 1; mal vol Approx final solvent ratio % vol observation ml ml Water/propylene glycol/ ethanol 0 5 0/50/50 Solution 0 05 5 05 1/49.5/49.5 Ppt forms but re dissolves on mixing 0.21 5.26 5/47.5/47.5 Ppt forms. Solution remains cloudy after mixing Indeed because of this observation the use of anhydrous ethanol Is preferred.

Example formulations (non-limiting) according to the invention are as follows: 1S COMPOSITION l (General) COMPONENT AMOUNT PER UNIT (Iml) FUNCTION Active Active THC (BDS) 25-50 mg/ml CBD (BDS) 25-50 mg/ml Excipient Propylene Glycol Peppermint oil 0.5 ml/ml Co solvent Ethanol (anhydrous) 0.0005 ml/ml Flavour qs to I ml Solvent COMPOSITION 2 (High THC) COMPONENT AMOUNT PER UNIT (Iml) FUNCTION Active THC (BDS) 25 mg/ml Active Excipient Propylene Glycol Peppermint oil 0.5 ml/ml Co solvent Ethanol (anLydrous) 0.0005 ml/ml Flavour qs to I ml Solvent

- 27 COMPOSITION 3 (High CBD) COMPONENT AMOUNT PER UNIT (Iml) FUNCTION Active CBD (BDS) 25 mg/ml Active Excipient Propylene Giycol Peppermint oil 0.5mUml Co solvent Ethanol (anhydrous) 0.0005ml/ml Flavour qs to I ml Solvent 5 COMPOSITION 4 (THC/CBD substantially l:l) COMPONENT AMOUNT PER UNIT (Iml) FUNCTION Active THC (BDS) 25 mg/ml Active CBD (BDS) 25 mg/ml Active Excipient Propylene Glycol Peppermint oil 0.5ml/ml Co solvent Ethanol (anhydrous) 0.0005ml/ml F1avour qs to I ml Solvent / Example 3

The following example illustrates the application of liquid spray formulations to the buccal mucosae and the blood levels produced by buccal absorption in comparison with sublingual administration.

IS The following liquid formulations suitable for buccal administration contain self-emulsifying agents, and hence do not fall within the scope of the present invention. Nevertheless, the general principles illustrated by use of these compositions 20 applies equally to the delivery formulations according to the invention. Solutions were produced by dissolving (at a temperature not exceeding 50 C) the following ingredients (quantitative details are expressed as parts by weight): A B C D E

Glyceryl monostearate (self-emulsifying) 2 2 2 Glyceryl monooleate (selfemulsifying) - 2 2 Cremophor RH40 20 30 30 20 30 CBME-G I to give THC S 10

- 28 -

CBME-G5 to give CBD - - 5 10 CBME-G I and G5 to give THC & CBD - - - each a-Tocopherol 0.1 0.1 0.1 0.1 0.1 Ascorbyl palmitate 0.1 0 1 0.1 0. 1 0.1 Ethanol BP to produce 100 100 100 100 100 Cannabis Based Medicine Extract (CBME) is an extract of cannabis which may be prepared by, for example, percolation with liquid carbon dioxide, with the removal of ballast by cooling a 5 concentrated ethanolic solution to a temperature of -20 C and removing precipitated Inert plant constituents by filtration or centrifugation. The product formed by mixing these Ingredients is 10 dispensed in 6 ml quantities into a glass vial and closed with a pump action spray. In use, the dose is discharged through a break-up button or conventional design. Proprietary devices that are suitable for this purpose are Type VP7 produced by Valois, but similar designs are available from other IS manufacturers. The vial may be enclosed in secondary packaging to allow the spray to be directed to a particular area of buccal mucosa. Alternatively, a proprietary button with an extension may be used to direct the spray to a preferred area of buccal mucosa. Each 1 ml of product contains 50-lOOmg of A9-tetrahydrocannabinol (THC) and/or cannabidiol (CBD). Each actuation of the pump delivers a spray which can be directed to the buccal mucosae. In the above formulations CBMEs of known 25 cannabinoid strength are used. CBME-G1 is an extract from a high THC-yielding strain of cannabis, and CBME-G5 is from a high CBD-yielding variety. It will be clear to a person skilled in the art that purified cannablnolds, and extracts containing the cannabinoids, can be made formulated as described above by 30 quantitative adjustment.

Although solutions of COME in ethanol alone can be used as a spray, the quantity of cannabinoid that can be delivered is limited by the aggressive nature of pure ethanol in high

- 29 concentratlon as a solvent. This limits the amount that can be applied to the mucosae without producing discomfort to the patient. When a group of patients received THC or CBD in a solution of the type described above, directing the spray either 5 sublingually or against the buccal mucosa, the patients uniformly reported a stinging sensation with the sublingual application, but mild or no discomfort when the same solution was sprayed onto the buccal mucosa. Spraying small quantities of this type of formulation onto the buccal mucosa does not 10 appreciably stimulate the swallowing reflex. This provides greater dwell time for the formulation to be in contact with the buccal surface.

Formulations were administered to a group of 13 human 15 subjects so that they received 4mg THC, 4mg of CBD or placebo (vehicle alone) via a sublingual tablet, sublingual pump-action spray or buccal route.

Absorption [area under the absorption curve (AUC)] of 20 cannablnoid and primary metabolite were determined in samples of blood taken after doslog. The following Table 8 gives these as normalized mean values.

Table 8

Route of Administration Analyte in Plasma PAS sublingual Sublingual tablet Oropharyngeal AUC AUC AUC

THC 2158.1 1648.4 1575

11-OH THC 3097 6 3560.5 2601.1

CBD 912 886 1 858

These results show that the total amounts of cannabinoid absorbed by sublingual and buccal (oropharyngeal) routes are similar but that there is a substantial (approximately 25%) reduction in the amount of 11- hydroxy (11-OH) metabolite 30 detected after oropharyogeal (buccal) administration. This finding is not inconsistent with reduced swallowing (and

- 30 subsequent reduced hepatic) metabolism of the buccal -

formulatlon. It is known that the ll-hydroxy metabolite of THC (ll-OH 5 THC) is possibly more psychoactive than the parent compound. It is therefore desirable to minimise the amount of this metabolite during administration, and this is likely to be achieved by using a formulation and method of application which reduces the amount of a buccal or sublingual dose that is swallowed. The: 10 pump action spray appears to offer a simple means of reducing the amount of material that is swallowed and metabolized by absorption from the intestinal tract below the level of the oropharynx. Example 4-Growing of_Medicinal Cannabis Plants are grown as clones from germinated seed, under glass at a temperature of 25 C + 1.5 C for 3 weeks in 24 hour -

daylight; this keeps the plants in a vegetative state.

20 Flowering is induced by exposure to 12 hour day length for 8-9 weeks. No artificial pesticides, herbicides, insecticides or fumigants are used. Plants are grown organically, with 25 biological control of insect pests.

The essential steps in production from seed accession to dried Medicinal Cannabis are summarized as follows: 30 Seed Accessions Seeds germinated at G-Pharm (UK) Selection for cannabinoid content and vigour 35 1 Mother Plant Cuttings rooted

- 31 14-21 days in peat plug 25 C, 24 hour day length Rooted cuttings potted up in 5 litre pot. of bespoke compost 5 1 Young Clone Plant AS tabllShed 3 weeks, 24 hour day length, 25 C Lower Branches Removed end of week 3 10 Used to make new generation of cuttings Induction of flowering Plant relocation to 12 hour day length are to induce flowering 15 Flower formation and maturation 8-9 weeks at 25 C Harvest 90% of flowers and leaves senesced 20 1 Drying Under conditions of light exclusion MEDICINAL CANNABIS

I Example 5-Determination of Cannabinoid Content in Plants and E tracts 30 Identity bv TLC a) Materials and methods Equipment Application device capable of delivering an 35 accurately controlled volume of solution i.e 1 pi capillary pipette or micro litre syringe.

TLC development tank with lid

- 32 Hot air blower Silica gel G TEC plates (SIL N-HR/UV254), 200 Am 5 layer with fluorescent indicator on polyester support. Dipping tank for visualization reagent.

10 Mobile phase 80% petroleum ether 60:80/20% Diethyl ether.

Visualisation reagent 0.1% w/v aqueous Fast Blue B (lOOmg in lOOml deionised water). An optional method is to scan at UV 15 254 and 365 nm.

b) Sample preparation i i) Herbal raw material Approximately 200mg of finely ground, dried cannabis is weighed into a lOml volumetric flask. Make up to volume using methanol:chloroform (9:1) extraction solvent.

25 Extract by ultrasound for 15 minutes. Decant supernatant and use directly for chromatography.

ii) Herbal drug Extract 30 Approximately 50mg of extract is weighed into a 25ml volumetric flask. Make up to volume using methanol solvent. Shake vigorously to dissolve and then use directly for chromatography.

35 c) Standards 0.1 mg/ml delta-9-THC in methanol.

O.lmg/ml COD in methanol.

- 33 The standard solutions are stored frozen at -20 C between uses and are used for up to 12 months after initial preparation.

5 d) Test solutions and method Apply to points separated by a minimum of 10mm.

i) either 5 pi of herb extract or 1 pi of herbal extract 10 solution as appropriate, ii) 10 1 of 0.1 mg/ml delta-9-THC in methanol standard solution, iii) 10 1 of 0.lmg/ml CBD in methanol standard solution.

15 Elute the TLC plate through a distance of 8cm, then remove the plate. Allow solvent to evaporate from the plate and then repeat the elusion for a second time (double development). 20 The plate is briefly immersed in the Fast Blue B reagent until the characteristic re/orange colour of cannabinoids begins to develop. The plate is removed and allowed to dry under ambient conditions in the dark. 25 A permanent record of the result is made either by i reproduction of

the Image by digital scanner(preferred | option) or by noting spot positions and colours on a tracing paper.

30 Assay THC, THCA, CBD, CBDA and CON by HPLC a) Materials and methods Equipment: HP 1100 HPLC with diode array detector and autosampler. The equipment is set up and 35 operated in accordance with in-house standard operating procedures (SOPlabO37) HPLC column Discovery ca 5 m, 15x 0.46 cm plus Kingsorb

- 34 ODS2 precolumn 5 Am 3 x 0.46 cm.

Mobile Phase Acetonotrile:methanol:0.25t aqueous acetic acid (16:7:6 by volume) Column Operatlng 25 C Temperature Flow Rate 1.0 ml/mln Injection Volume 10 Ill Run time 25mins 15 Detection Neutral and acid cannabinolds 220nm (band width 16nm) Reference wavelength 400nm/bandwidth 16nm 20 Slit 4nm Acid cannabinoids are routinely monitored at 310nm (band width 16nm) for qualitative confirmatory and 25 identification purposes only.

Data capture HP Chemistation with Version A7.01 software 30 b) Sample preparation Approximately 40mg of Cannabis Based Medicinal Extract is dissolved in 25ml methanol and this solution is diluted to 1 to 10 in methanol.

35 This dilution is used for chromatography.

0.5 ml of the fill solution, contained within the Pump Action Sublingual Spray unit, is

- 35 sampled by glass pipette. The solution is diluted into a 25ml flask and made to the mark with methanol.

200 Al of this solution is diluted with 800 Al 5 of methanol.

Herb or resin samples are prepared by taking a l00mg sample and treating this with 5 or l0ml of Methanol/Chloroform (9/l w/v). The 10 dispersion is sonlcated in a sealed tube for lo minutes, allowed to cool and an aliquot is centrifuged and suitably diluted with methanol prior to chromatography.

15 c) Standards External standardisatlon is used for this method. Dilution of stock standards of THC, CBD and CBN in methanol or ethanol are : made to give final working standards of approximately 20 accurately 0.l mg/ml. The working standards are stored at -20 C and are used for up to 12 months after initial preparation.

Injection of each standard is made in triplicate prior to the inlectlon of any test solution. At suitable intervals during 25 the processing of test solutions, repeat injections of standards are made. In the absence of reliable CBDA and THCA standards, ! these compounds are analysed using respectively the CBD and THC standard response factors.

30 The elusion order has been determined as CBD, CBDA, CBN, THC and THCA. Other cannabinoids are detected using this method and may be identified and determined as necessary.

d) Test solutions Diluted test solutions are made up in methanol and should contain analyses in the linear working range of 0.02-0.2 mg/ml.

!

- 36 e) Chromatography Acceptance Criteria: The following acceptance criteria are applied to the results of each sequence as they have been found to result in adequate 5 resolution of all analyses (including the two most closely elating analyses CBD and CBDA) i) Retention time windows for each analyte: 10 CBD 5.4-5.9 minutes CBN 7.9-8.7 minutes THC 9.6-10.6 minutes ii) Peak shape (symmetry factor according to BP method) CBD < 1.30

CBN < 1.25

THC < 1.35

20 iii) A number of modifications to the standard method have been developed to deal with those samples which contain late eluting impurity peaks e.g method CBD2A extends the run time to 50 minutes. All solutions should be clarified by centrifugation before being 25 transferred into autosampler vials sealed with teflon faced septum seal and cap.

iv) The precolumn is critical to the quality of the chromatography and should be changed when the back 30 pressure rises above 71 bar and/or acceptance criteria regarding retention time and resolution, fall outside their specified limits.

f) Data Processing Cannabinoids can be subdivided into neutral and acidicthe qualitative identification can be performed using the DAD dual wavelength mode. Acidic cannabinoids absorb strongly in the

- 37 region of 220nm-310nm. Neutral cannabinoids only absorb strongly in the region of 220nm.

Routinely, only the data recorded at 220 nm is used for 5 quantitative analysis.

The DAD can also be set up to take UV spectral scans of each peak, which can then be stored in a spectral library and used for identification purposes.

Data processing for quantitation utilizes batch processing software on the Hewlett Packard Chemstation.

a) Sample Chromatograms 15 HPLC sample chromatograms for THC and COD Herbal Drug extracts are provided in the accompanying Figures.

Example 6-Preparation of the Herbal Drug Extract A flow chart showing the process of manufacture of extract from the High-THC and Hlgh-CBD chemovars is given below: Medicinal Cannabis tHigh-THc or High-CBD) 25 1 Chopping to predominantly 2 to 3mm ! 1 Heating at 100 to 150 C for sufficient time to decarboxylate acid form of 30 cannabinoids to produce neutral cannabinoids Extraction with a specified volume of liquid carbon dioxide over 6 to 8 hours 35 Removal of CO2 by depressurlsation to recover crude extract Wlnterisatlon''-Dlssolution of crude extract in ethanol Ph. Eur.

- 38 followed by chilling solution (-200C/4B furs) to precipitate unwanted waxes Removal of unwanted waxy material by cold filtration 5 1 Removal of ethanol from the filtrate by thin film evaporation under reduced pressure The resulting extract is referred to as a Cannabis Based 10 Medicine Extract and is also classified as a Botanic Drug Substance, according to the US Food and Drug Administration Guidance for Industry Botanical Drug Products.

Example 7

15 High THC cannabis was grown under glass at a mean temperature of 21 + 2 C, RH 50-60%. Herb was harvested and dried at ambient room temperature at a RH of 40-95% in the dark. When dry, the leaf and flower head were stripped from stem and this dried biomass is referred to as "medicinal cannabis".

Mediclnal cannabis was reduced to a coarse powder (particles passing through a 3 mm mesh) and packed into the chamber of a Supercrltical Fluid Extractor. Packing density was 0.3 and liquid carbon dioxide at a pressure of 600 bar was 25 passed through the mass at a temperature of 35 C. Supercritical extraction is carried out for 4 hours and the extract was recovered by stepwise decompression into a collection vessel.

The resulting green-brown oily resinous extract is further purified. When dissolved in ethanol BP (2 parts) and subjected 30 to a temperature of 20 C for 29 hours a deposit (consisting of fat-soluble, waxy material! was thrown out of solution and was removed by filtration. Solvent was removed at low pressure in a rotary evaporator. The resulting extract is a soft extract which contains approximately 60% THC and approximately 6% of 35 other cannabinoids of which 1-2 is cannabidiol and the remainder is minor cannabinoids including cannabinol.

Quantitative yield was 9% w/w based on weight of dry medicinal cannabis.

- 39 A high CBD chemovar was similarly treated and yielded an extract containing approximately 60% CBD with up to A% tetrahydrocannabinol, within a total of other cannabinoids of 5 6%. Extracts were made using THCV and CBDV chemovars using the general method described above.

A person skilled in the art will appreciate that other combinations of temperature and pressure (e.g. in the range +10 C 10 to 35 C and 60-600 bar) can be used to prepare extracts under supercritical and subcritical conditions.

i Example 8- The Effects of Light on the Stability of the Alcoholic Solutions of THC,CBD or THCV.

The following example includes data to support the packaging of liquid dosage forms in amber glass, to provide some protection from the degradative effects of light on cannabinolds. Further credence is also given to the selection of the lowest possible storage temperature for the solutions containing cannabinoid active ingredients.

25 Background and Overview:

Light is known to be an initiator of degradation reactions in many substances, including cannabinoids. This knowledge has | been used in the selection of the packaging for liquid formulations, amber glass being widely used in pharmaceutical 30 presentations as a light exclusive barrier.

Experiments were set up to follow the effects of white light on the stability of methanolic solutions of THC, CBD or THCV. Following preliminary knowledge that light of different 35 wavelengths may have differing effects on compound stability (viz. tretinoin IS stable only in red light or darkness), samples were wrapped in coloured acetate films or in light exclusive foil. A concurrent experiment used charcoal treated CBME to study the effects of the removal of plant pigments on 40 the degradation process.

l

- 40 Materials and Methods: Cannabinoids: 1 mgJml solutions of CBME were made up in 5 AR methanol. Methanolic solutions of CBME (lOOmg/ml) were passed through charcoal columns (Biotage Flash 12AC 7.5cm cartridges, lo/no. 273012S) and were then diluted to lmg/ml.

Solutions were stored in soda-glass vials, which were tightly screw capped and oversealed with stretch film. Tubes were 10 wrapped in coloured acetate films as follows: Red, Yellow, Green, and Cyan Solutions were also filled into the amber glass U-save 15 vials; these were sealed with a septum and oversealed. One tube of each series of samples was tightly wrapped in aluminium foil in order to completely exclude light. This served as a "dark" control to monitor the contribution of ambient temperature to the degradation behaviour. All of the above tubes were placed 20 in a box fitted with 2 x 40 watt white Osram fluorescent tubes.

The walls of the box were lined with reflective foil and the internal temperature was monitored at frequent intervals.

A further tube of each series was stored at -20 to act as 25 a pseudo to the reference sample; in addition, one tube was exposed directly to light without protection. Samples were withdrawn for chromatographic analysis at intervals up to 112 days following the start of the study. The study was designated AS01201/AX282.

Samples of the test solutions were withdrawn and diluted as appropriate for HPLC and TLC analysis. HPLC was carried out In accordance with TM GE. 004.V1 (SOPamO58). TLC was performed on layers on Silica gel (MN SilG/UV) in accordance with TM 35 GE.002.V1 (SOPamO56).

Two further TLC systems were utilized in order to separate degradation products: 40 a) SllG/UV, stationary phase, hexane/acetone 8/2 v/v mobile

- 41 phase b) RPCl8 stationary phase, acetonitrile/methanoltO.25% aqueous acetic acid 16/7/6 by volume Visualisation of cannabinoids was by Fast Blue B salt.

Results and Discusslon: HPLC quantitative analysis: 10 The results from the HPLC analysis of samples drawn from the stored, light exposed solutions, are plotted and presented as Figures 6, 6a (THC before and after charcoal treatment), and 7, 7a (CBD before and after charcoal treatment).

tS It can be seen from Figs. 6 and 6a that there are significant improvements to the stability of THC in all solutions, except those stored in the dark (at ambient temperature) and at -20 (and hence which are not under photochemical stress). Even storage in amber glass shows an 20 improvement when un-treated extract IS compared with charcoal treated extract. This, however, may reflect in an improvement of the thermal stability of the charcoal treated extract.

Figs. 7 and 7a present similar data for CBD containing 25 extracts, from which it can be seen that this cannabinoid is significantly more Sensitive to the effects of light than is THC. In the absence of charcoal, all exposures, except in amber glass, light excluded (foil) and -20 storage, had degraded to non-detectable levels of CBD before 40 days. This improved to 30 figures of between 92 and 62 days following charcoal treatment.

Amber glass protected CBD showed an improvement from 38% residual compound at 112 days without charcoal clean up, to approximately 64% at the same time after charcoal treatment.

There was also an improvement in the stability of CBD in light 35 excluded solution after charcoal treatment. This can only reflect a reduction in either thermo-oxidative degradation, or a residual photochemical degradation initiated by light (and/or air) during COME and solution preparation.

40 Thin Layer Chromatography Qualitative Analysis:

* - 42 The evaluation of the light degraded solutions using thin layer chromatography, used both the existing normal phase system li.e. Silica stationary phase and hexane/diethyl ether as mobile phase) and two additional systems, capable of resolving more 5 polar or polymeric products formed during the degradation processes. Thus, chromatography using the hexane/diethyl ether system, showed that for THC by day 112, there was a reduction in 10 the intensity of the THC and secondary CBD spots with all of the colour filtered lights (data not shown). At the same time, there was an increase in the intensity of Fast Blue B stalalog material running at, or close to, the origin. Foil protected solution exhibited none of these effects.

Conclusions and Recommendations:

Cannabinoids are known to be degraded by a number of natural challenges, VIZ. light, heat, oxygen, enzymes etc. It is most likely that in an extract of herbal plant material, 20 which has not been subjected to extensive clean-up procedures, that some of these processes may still be able to continue.

Paradoxically, it IS also likely that the removal of cannabinoids from the presence of any protection agents within the plant tissue, may render the extract more likely to suffer 25 from particular degradation pathways.

Packaging into amber glass vials, conducting formulation I manufacture in amber filtered light, and the storage of plant | extracts and pharmaceutical formulations at temperatures as low 30 as possible compatible with manufacturing and distribution requirements and patient compliance eliminates, or at least reduces, the effect of light on degradation of cannabinoids.

These actions dramatically improved the storage stability of both plant extracts and finished products.

It was interesting to note that COD appeared to be markedly less stable than THC, when subjected to photochemlcal stress. This is the opposite of the finding for the relative thermo-oxidative stabilities, in which THC is the less stable.

40 This seems to indicate that, although polymeric degradation

- 43 products may be the common result of both photochemical and thermooxidative degradation, the exact details of the mechanism are not identical for the two processes.

5 Among the conclusions that can be drawn are the following:

1] The choice of amber glass for the packaging of the dose solutions provides improved stability, but minor improvements can be made by additional light exclusion measures.

21 The drying process and subsequent extraction and formulation of cannabis extracts should indeed be carried out in low intensity, amber filtered light.

15 3] Consideration should be given to the blanketing of extracts under an inert atmosphere (e.g. Nitrogen).

41 Clean-up of cannabis extracts by simple charcoal filtration after winterisation, may yield substantial 20 improvements to product shelflife.

Claims (79)

1. t! - 44 C1aims: 1. A liquid pharmaceutical formulation, for use in

   administration of a lipophilic medicament via a mucosal surface, 5 comprising: at least one lipophilic medicament, a solvent and a cosolvent, wherein the total amount of solvent and co-solvent present in the formulation is greater than 55% wt/wt of the formulation and the formulation is absent of a self emulsifying agent and/or a fluorinated propellant.
2. 2. A liquid pharmaceutical formulation as claimed in claim 1 with a water content of less than 5.
3. 3. A liquid pharmaceutical formulation as claimed in 15 claim 1 or 2 wherein the solvent is selected from C1-C4 alcohols.
4. 4. A liquid pharmaceutical formulation as claimed in claim 3 wherein the solvent is ethanol.
5. 5. A liquid pharmaceutical formulation as claimed in any of the preceding claims wherein the co-solvent is selected from glycols, sugar alcohols, carbonate esters and chlorinated hydrocarbons.
6. 6. A liquid pharmaceutical formulation as claimed in claim 5 wherein the glycols are selected from propylene glycol and glycerol, and the carbonate ester is propylene carbonate.

   30
7. 7. A liquid pharmaceutical formulation as claimed in claim 5 wherein the co-solvent is propylene glycol.
8. 8. A liquid pharmaceutical formulation as claimed in claim 1 or 2 wherein the solvent IS ethanol and the co-solvent 35 IS propylene glycol.
9. 9. A liquid pharmaceutical formulation as claimed in claim 8 wherein ethanol/propylene glycol are present in relative

   - 45 proportlons by weight in the range 60/40 to 40/60.
10. 10. A liquid pharmaceutical formulation as claimed in claim 8 wherein ethanol/propylene glycol are present in relative 5 proportions by weight in the range 55/45 to 45/55.
11. 11. A liquid pharmaceutical formulation as claimed in claim 8 wherein ethanol/propylene glycol are present in the relative proportions by weight of about 50/50.
12. 12. A liquid pharmaceutical formulation as claimed in any of the preceding claims further comprising a flavouring.
13. 13. A liquid pharmaceutical formulation as claimed in Is claim 12 wherein the flavouring is peppermint oil.
14. 14. A liquid pharmaceutical formulation as claimed in claim 12 or claim 13 wherein the flavouring is present in an amount up to 0.1% v/v, preferably 0.05% v/v.
15. 15. A liquid pharmaceutical formulation as claimed in any of the preceding claims wherein the lipophilic medicament comprises one or more cannabinoids.

    25
16. 16. A liquid pharmaceutical formulation as claimed in claim 15 wherein the 1lpophilic medicament is at least one extract from at least one cannabis plant.
17. 17. A liquid pharmaceutical formulation as claimed in 30 claim 16 wherein the extract from at least one cannabis plant is a botanical drug substance.
18. 18. A liquid formulation as claimed in claim 17 wherein the botantlcal drug substance has undergone an extraction step 35 to remove a substantial proportion of waxes and other solvent insoluble materials present in plant material.
19. 19. A liquid formulation as claimed in claim 17 or 18

    - 46 wherein the botanical drug substance Is a CO2 extract of one or more cannibis plants.
20. 20. A liquid pharmaceutical formulation as claimed in 5 any of one the preceding claims consisting essentially of one or more cannabinoids, ethanol and propylene glycol, and optionally a flavouring and/or an opiate.
21. 21. A liquid pharmaceutical formulation wherein the 10 lipophilic medicament comprises tetrabydrocannabinol, A9 tetrabydrocannablnol, A8tetrahydrocannabinol, 69 tetrahydrocannablool propyl analogue, cannabidiol, cannabldiol propyl analogue, cannabinol, cannablchromene, cannabichromene propyl analogue, cannabigerol or any mixture thereof.
22. 22. A liquid pharmaceutical formulation as claimed in any one of claims 15 to 21 wherein the cannabinoids include THC and/or CBD.

    20
23. 23. A liquid pharmaceutical formulation as claimed in claim 22 wherein THC and CBD are present in a ratio (w/w) of from 0.9:1.1 to 1.1:0.9.
24. 24. A liquid pharmaceutical formulation as claimed in 25 claim 22 wherein THC and CBD are present in a ratio (w/w) of about l:1.
25. 25. A formulation according to claim 24 which formulation includes 2.5 g/ml of each of THC and CBD.
26. 26. A liquid pharmaceutical formulation as claimed in any one of claims 15 to 21 which comprises both the cannabinoids cannabiaiol (CBD) and tetrahydrocannabinol (THC), or the cannabinoids tetrabydrocannabinovarin (THCV) and cannabidivarin 35 (CBDV), in a pre-defined ratio by weight.
27. 27. A pharmaceutical formulation according to claim 26 which comprises both the cannabinoids cannabidiol (CBD) and

    - 47 tetrahydrocannabinol (THC), wherein the CBD is present in an amount by weight which is greater than the amount by weight of THC. 5
28. 28. A formulation according to claim 27 wherein the ratio by weight of CBD to THC is greater than 2.5:1.
29. 29. A formulation according to claim 27 or claim 28 wherein the ratio by weight of CBD to THC is between 99:1 and 10 2.5:1, preferably between about 20:1 and about 2.5:1.
30. 30. A formulation according to any one of claims 27 to 29 wherein the ratio by weight of CBD to THC is about 19:1.

    15
31. 31. A formulation according to any one of claims 27 to 29 wherein the ratio by weight of CBD to THC is in the range from about 5:1 to about 3:1.
32. 32. A formulation according to any one of claims 27 to 20 31 which is substantially free of cannabinoids other than CBD and THC.
33. 33. A formulation according to claim 32 which is substantially free of other cannabinoids found in Cannabis sp.
34. 34. A formulation according to any one of claims 27 to 33 wherein the CBD and THC are in substantially pure form.
35. 35. A formulation according to any one of claims 27 to 30 31 which further comprises one or more other cannabinoids.
36. 36. A formulation according to claim 35 wherein the one or more other cannabinoids are tetrabydrocannabinovarin (THCV) and/or cannabidlvarin (CBDV).
37. 37. A formulation according to any one of claims 27 to 31, 35 or 36 wherein the CBD and THC form part of least one extract from at least one Cannabis plant, said at least one

    - 48 extract comprising all the naturally occurring cannabinoids in said plant.
38. 38. A formulation according to claim 37 wherein the 5 Cannabis plant is selected from Cannabis saliva, Cannabis lndica, a genetic cross between them, a self-cross or a hybrid thereof.
39. 39. A formulation according to claim 38 wherein the 10 Cannabis plant Is Cannabis saliva, subspecies indlca and is selected from var. indlca and var. kaflristanica.
40. 40. A formulation as claimed in any one of claims 37 to 39 which comprises extracts from two or more different Cannabis lS varieties wherein in the final formulation the amount of CBD IS greater than the amount of THC by weight.
41. 41. A formulation according to any one of claims 37 to 39 wherein said extract is prepared by supercritical or 20 sub-critical fluid extraction of dried Cannabis plant.
42. 42. A liquid pharmaceutical formulation according to claim 26 which comprises both the cannabinoids cannabidiol (CBD) and tetrabydrocannabinol (THC), wherein the THC is present in an 25 amount by weight which is greater than the amount by weight of CBD.
43. 43. A formulation according to claim 42 wherein the pre-defined ratio by weight of CBD to THC is between 1:99 and 30 1:1 5
44. 44. A formulation according to claim 43 wherein said pre-defined ratio by weight of COD to THC is about 1:39.

    35
45. 45. A formulation according to claim 43 wherein said pre-defined ratio by weight of CBD to THC is about 1:2.
46. 46. A pharmaceutical formulation according to claim 26

    1. - 49 which comprises both the cannabinoids tetrahydrocannabinovarin (THCV) and cannabidivarin (CBDV) wherein the CBDV is present in an amount by weight which is greater than the amount by weight of THCV.

    S
47. 47. A formulation according to claim 46 which further comprises CBD and/or THC.
48. 48. A formulation according to claim 46 or 47 wherein 10 the ratio by weight of CBDV to THCV is greater than 1.5:1.
49. 49. A formulation according to any one of claims 46 to 48 wherein the ratio by weight of CBDV to THCV is in the range from about 99:1 to about 1.5:1, preferably from about 20:1 to 15 about 2.5:1.
50. 50. A formulation according to any one of claims 46 to 49 wherein the ratio by weight of CBDV to THCV is about 9:1.

    20
51. 51. A formulation according to any one of claims 46 to 49 wherein the ratio of CBDV to THCV by weight is from about 5:1 to 3:.
52. 52. A formulation according to claim 46 or any one of 25 claims 48 to 51 which is substantially free from other cannabinoids found in Cannabis sp.
53. 53. A formulation according to any one of claims 46 to 52 wherein the CBDV and THCV form part of an extract from a 30 Cannabis plant, said extract comprising all of the naturally occurring cannablnoids in said plant.
54. 54. A formulation according to claim 53 wherein the Cannabis plant is selected from Cannabis saliva, Cannabis indica 35 or a genetic cross between them, a self-cross or a hybrid thereof.
55. 55. A liquid pharmaceutical formulation as claimed in

    - 50 -

    any one of claims 15 to 21 which comprises both the cannabinoids THC and TIlCV wherein the THCV is present in an amount by weight which is approximately equal to or greater than the amount by weight of THC.
56. 56. A pharmaceutical formulation according to claim 55 wherein the ratio by weight of THCV to THC is between 99:1 and 1.5:1.

    10
57. 57. A formulation according to claim 55 or 56 wherein the ratio by weight of THCV to THC IS approximately 17:3.
58. 58. A formulation according to any one of claims 55 to 57 which also comprises COD and/or CBDV at an amount by weight 15 which is less than the amount by weight of THCV.
59. 59. A formulation according to any one of claims 55 to 58 wherein the THCV and THC form part o f an extract from a Cannabis plant, said extract comprising all the naturally 20 occurring cannabinoids in said plant.
60. 60. A formulation according to claim 59 wherein said Cannabis plant is selected from Cannabis saliva, Cannabis indica or the result of a genetic cross between them, a self-cross or a 2S hybrid thereof.
61. 61. A pharmaceutical formulation according to any one of claims 26 to 42, 46 to 59 for use in the treatment of inflammatory disease or any disease or condition during the 30 course of which oxidative stress plays a part.
62. 62. A pharmaceutical formulation according to claim 32 or 51 for use in the treatment of rheumatoid arthritis, or inflammatory bowel disease or Crohn's disease.
63. 63. A pharmaceutical formulation according to claim 31 or SO for use in the treatment of psychotic disorders, epilepsy, movement disorders, stroke, head injury, or diseases which

    - 51 require appetite suppression.
64. 64. A pharmaceutical formulation according to any one of claims 15 to 21 which comprises approximately equal amounts of 5 CBD and THC or THCV and CBDV for the treatment of multiple sclerosis, spinal cord injury, peripheral neuropathy or other neurogenic pain.
65. 65. A pharmaceutical formulation according to any one of 10 claims 15 to 21 Welch comprises a ratio by weight of THC to CBD I or THCV to CBDV of from about 39:1 to about 99:1 for use in the treatment of cancer pain or migraine or for stimulation of appetite. 15
66. 66. A pharmaceutical formulation for use according to claim 65 wherein the ratio by weight of THC to CBD or THCV to CBDV is approximately 39:1.
67. 67. A pharmaceutical formulation for use according to 20 claim 65 or 66 wherein in the formulation the THC and CBD and/or THCV and CBDV form part of an extract from a Cannabis plant, said extract comprising all the naturally occurring cannabinoids in said plant.

    25
68. 68. A liquid pharmaceutical formulation comprising in a 1 ml vol: THC 25 mg/ml (based on amount of cannabinoid in a botanical drug substance), CBD 25 mg/ml (based on amount of cannabinoid in a botanical drug substance), propylene glycol 0.5 ml/ml, peppermint oil 0.0005 ml/ml, and ethanol (anhydrous) qs 30 to 1 ml.
69. 69. A liquid pharmaceutical formulation comprising in a l ml vol: THC 25 mg/ml (based on amount of cannabinoid in a botanical drug substance), propylene glycol 0.5 ml/ml, 35 peppermint oil 0.0005 ml/ml, and ethanol (anhydrous) qs to 1 ml.
70. 70. A liquid pharmaceutical formulation comprising in a 1 ml vol: CBD 25 mg/ml (based on amount of cannabinoid in a

    J - 52 botanical drug substance), propylene glycol 0.5 ml/ml, peppermint oil 0.0005 ml/ml, and ethanol (anNydrous) qs to 1 ml.
71. 71. A liquid formulation as claimed in any of claims 1 5 to 14 wherein the lipophilic medicament is selected from: morphine, pethidine, codeine, methadone, diamorphine, fentanyl, alfentanil, temazepam, buprenorphine, lipophilic analgesics and drugs of abuse.
72. 72. A liquid formulation as claimed in any of the preceding claims which is packaged for delivery as a spray having a mean aerodynamic particle size, when delivered, of from 20-40 microns, more preferably 25-35 and most preferably 30-35 microns.
73. 73. A 1lquld formulations as claimed in claim 72 which is packaged for delivery as a pump-action spray.
74. 74. A liquid formulation as claimed in claim 73 wherein 20 the formulation is dispensed through a mechanical pump.
75. 75. A liquid formulation as claimed in claim 74 wherein the mechanical pump comprises a VP7 actuator valve.

    25
76. 76. A liquid formulation as claimed in any of the preceding claim wherein the formulation is packaged in a container coloured to omit UV light and light from the blue region of the spectrum, preferably at wavelengths in the range of 200-500 nm.
77. 77. A liquid formulation as claimed in claim 76 wherein the container is coloured amber.
78. 78. A liquid formulation as claimed in any of the 35 preceding claims wherein the lipophilic medicament is packaged In an inert atmosphere.
79. 79. A liquid formulation as claimed in claim 78 wherein

    - 53 the lipophilic medicament is packaged under nitrogen.