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GB2383045A - Novel cell wall anchor proteins derived from yeast, genes thereof and cell surface expression systems using the same - Google Patents

Novel cell wall anchor proteins derived from yeast, genes thereof and cell surface expression systems using the same

Info

Publication number
GB2383045A
GB2383045A GB0301013A GB0301013A GB2383045A GB 2383045 A GB2383045 A GB 2383045A GB 0301013 A GB0301013 A GB 0301013A GB 0301013 A GB0301013 A GB 0301013A GB 2383045 A GB2383045 A GB 2383045A
Authority
GB
United Kingdom
Prior art keywords
protein
cell surface
cell wall
surface expression
genes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0301013A
Other versions
GB0301013D0 (en
Inventor
Eui-Sung Choi
Jung-Hoon Sohn
So-Young Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Research Institute of Bioscience and Biotechnology KRIBB
Original Assignee
Korea Research Institute of Bioscience and Biotechnology KRIBB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Research Institute of Bioscience and Biotechnology KRIBB filed Critical Korea Research Institute of Bioscience and Biotechnology KRIBB
Publication of GB0301013D0 publication Critical patent/GB0301013D0/en
Publication of GB2383045A publication Critical patent/GB2383045A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention provides novel cell wall anchor proteins derived from yeast, genes thereof and the genetic method for tethering polypeptide to the yeast cell wall using the same. Particularly, the present invention provides the novel GPI(glycosyl phosphatidyl inositol)-anchor protein genes, <I>SED1</I>, <I>GAS1</I>, <I>TIP1</I> and <I>CWP1</I>, and their proteins. <I>PIR2</I> cell wall protein gene and its protein derived from <I>Hansenula polymorpha</I>, and the cell surface expression system using them which immobilize foreign enzymes or polypeptides on the cell wall of a microbial cell. In addition, the present invention provides the cell surface expression system using <I>WSC1</I> gene and it s protein derived from yeasts, including <I>Hansenula polymorpha</I> and <I>Saccharomyces cerevisiae</I>, and <I>STA1</I> gene and its protein derived from <I>Saccharomyces diastaticus</I>. The cell surface expression system of the present invention expects an immobilization effect as biocatalysts by adhereing a desired protein to the cell surface, and provides a means of altering target protein characteristics such as binding affinity and stability by library screening.
GB0301013A 2000-07-26 2000-07-27 Novel cell wall anchor proteins derived from yeast, genes thereof and cell surface expression systems using the same Withdrawn GB2383045A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20000042939 2000-07-26
PCT/KR2000/000819 WO2002012509A1 (en) 2000-07-26 2000-07-27 Novel cell wall anchor proteins derived from yeast, genes thereof and cell surface expression systems using the same

Publications (2)

Publication Number Publication Date
GB0301013D0 GB0301013D0 (en) 2003-02-19
GB2383045A true GB2383045A (en) 2003-06-18

Family

ID=19679978

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0301013A Withdrawn GB2383045A (en) 2000-07-26 2000-07-27 Novel cell wall anchor proteins derived from yeast, genes thereof and cell surface expression systems using the same

Country Status (7)

Country Link
US (1) US7132273B1 (en)
KR (1) KR100395307B1 (en)
CN (1) CN100365125C (en)
AU (1) AU2000261856A1 (en)
DE (1) DE10085481T1 (en)
GB (1) GB2383045A (en)
WO (1) WO2002012509A1 (en)

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KR100475133B1 (en) 2002-09-13 2005-03-10 한국생명공학연구원 Method for screening of a lipase having improved enzymatic activity using yeast surface display vector and the lipase
WO2006088328A1 (en) * 2005-02-18 2006-08-24 Cgk Co., Ltd. Detection system for molecular interactions
KR100743973B1 (en) 2006-04-27 2007-07-30 한국과학기술원 Method of surface expression on cell surface simultaneously with enzyme that regenerates protein and cofactor
WO2008118476A2 (en) * 2007-03-26 2008-10-02 Codon Devices, Inc. Cell surface display, screening and production of proteins of interest
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
CA2715212A1 (en) * 2008-03-03 2009-09-11 Glycofi, Inc. Surface display of recombinant proteins in lower eukaryotes
US8067339B2 (en) 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
CN101717736B (en) * 2009-10-23 2013-06-19 中国科学院水生生物研究所 Ultrahigh permeability bread yeast and preparation method thereof
IL210093A0 (en) * 2010-12-19 2011-06-30 David Helman Membrane bound reporter molecules and their use in cell sorting
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
CN102260329B (en) * 2011-07-29 2013-09-25 华南理工大学 Pichia pastoris wall protein Gcw45, and surface display system and construction method thereof
KR101498012B1 (en) * 2012-06-19 2015-03-11 한국생명공학연구원 HpGAS1 gene deleted yeasts and methods of producing recombinant proteins using them
WO2013191369A1 (en) * 2012-06-19 2013-12-27 한국생명공학연구원 Hpgas1 gene-disrupted yeast strain and production method for recombinant protein using same
KR20170003787U (en) 2016-04-26 2017-11-03 주식회사 스튜디오오겐 The sturcture of nose pad of glasses
WO2018041740A1 (en) 2016-09-01 2018-03-08 Bayer Pharma Aktiengesellschaft Non-covalent display system using fimgt/dsf
WO2018167670A1 (en) * 2017-03-13 2018-09-20 Lallemand Hungary Liquidity Management Llc Recombinant yeast host cells expressing cell-associated heterologous proteins
CN108676811B (en) * 2018-05-28 2021-07-13 郝志敏 Gene traceless editing vector and application thereof in organism gene editing
US12247196B2 (en) 2018-09-20 2025-03-11 Washington University Engineered microorganisms and methods of making and using same
CN110093404A (en) * 2019-04-16 2019-08-06 艾美汉信疫苗（大连）有限公司 The method for screening recombination Hansenula yeast virus-like particle expression strain using fluorescence real-time quantitative PCR
CN110551644A (en) * 2019-07-19 2019-12-10 天津科技大学 low-yield higher alcohol saccharomyces cerevisiae strain constructed by regulating cell wall constituent protein
CN111996132A (en) * 2020-07-21 2020-11-27 郑州大学 A kind of surface display method of Yarrowia lipolytica
EP4271819A4 (en) * 2020-12-30 2025-03-19 Clara Foods Co. Surface displayed endoglycosidases
CA3203880A1 (en) * 2020-12-30 2022-07-07 Weixi ZHONG Surface displayed endoglycosidases
CN115232757B (en) * 2022-06-22 2023-05-23 首都师范大学 Saccharomyces cerevisiae strain, fermentation strain and its construction method and bioethanol production method
CN115160417B (en) * 2022-06-24 2023-06-02 西南大学 Glyco_hydro-like secreted protein of Pichia chelitatus and its application
US20240084243A1 (en) * 2022-06-29 2024-03-14 Clara Foods Co. Surface displayed fusion proteins
KR20240053728A (en) 2022-10-17 2024-04-25 주식회사 퓨처앤텍 Surface-display overexpression technique by using lipoprotein PrsA expression system
CN120272339B (en) * 2025-06-11 2025-08-19 上海欣瑞特生物医药技术有限公司 A recombinant engineered bacterium for preparing recombinant equine albumin and its application

Citations (4)

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Publication number Priority date Publication date Assignee Title
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
US5602034A (en) * 1987-12-30 1997-02-11 Chiron Corporation Expression and secretion of heterologous proteins in yeast employing truncated alpha-factor leader sequences
US6114147A (en) * 1993-02-10 2000-09-05 Unilever Patent Holdings Immobilized proteins with specific binding capacities and their use in processes and products

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5741672A (en) * 1984-07-27 1998-04-21 Unilever Patent Holdings B.V. Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes
KR100256023B1 (en) * 1997-10-24 2000-05-01 박호군 Multi-parallel introduction type expression vector for Hanshenula polymorpha
CN100338091C (en) * 1999-09-25 2007-09-19 伊藤火腿株式会社 Encoding blended protein DNA and preparing of useful polypeptides by expression thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5602034A (en) * 1987-12-30 1997-02-11 Chiron Corporation Expression and secretion of heterologous proteins in yeast employing truncated alpha-factor leader sequences
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
US6114147A (en) * 1993-02-10 2000-09-05 Unilever Patent Holdings Immobilized proteins with specific binding capacities and their use in processes and products

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Appl Environ Microbiol 1998 Dec;64(12);4857-61 *
Mol Cell Biol 2000 Mar;20(9):3245-55 *

Also Published As

Publication number Publication date
CN100365125C (en) 2008-01-30
KR20020010428A (en) 2002-02-04
GB0301013D0 (en) 2003-02-19
US7132273B1 (en) 2006-11-07
DE10085481T1 (en) 2003-07-31
KR100395307B1 (en) 2003-09-06
WO2002012509A1 (en) 2002-02-14
AU2000261856A1 (en) 2002-02-18
CN1454258A (en) 2003-11-05

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WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)