GB2373785A - Truncating chemokines using dipeptidyl peptidase IV - Google Patents

Abstract

A method for cleaving NH2-terminal dipeptides from the following CXC chemokines: interferon (IFN) inducible chemokines (= IFN-gamma inducible protein-10 (IP-10)); monokine induced by IFN-gamma (= Mig) and IFN-inducible T-cell alpha-chemoattractant (= I-TAC). The chemokines are truncated using the lymphocyte membrane protease dipeptidyl peptidase IV (= CD26 or DPP IV). The truncated chemokines have reduced CXCR3 receptor binding properties, loss of calcium signalling capacity through CXCR3, a reduced chemotactic potency but they retain the ability to inhibit angiogenic activity of cells. The truncated chemokines may be used in the treatment of diseases such as multiple sclerosis, B-cell leukemia, hepatocellular carcinoma and skin diseases.

Description

CHEMOKINES Background of the invention Chemokines constitute a family of low molecular mass proteins that regulate the directed migration of specific subclasses of leukocytes during normal and inflammatory processes (1-3). The cellular specificity of chemokines is determined by the restricted expression of chemokine receptors on various leukocyte cell types (4). Chemokines are divided in subfamilies depending on the position of the first two cysteines in their primary sequence.

The CC subfamily, with two adjacent cysteines, contains more than 20 different proteins that regulate the migration of monocytes, eosinophils, basophils, B and T lymphocytes, natural killer (NK) cells and dendritic cells. The CXC chemokine subfamily, with two cysteines separated by one other amino acid, contains several proteins with a Glu-Leu-Arg (ELR) motif in front of the first cysteine. These ELRCXC chemokines all attract neutrophilic granulocytes to sites of inflammation. The CXC chemokines without ELR-motif can attract monocytes, B and/or T lymphocytes. Three of the known non-ELR-CXC chemokines, i. e. the interferon-y

(IFN-y) inducible protein-10 (IP-10 or CXCL10), monokine induced by IFN-y (Mig or CXCL9) and IFN-inducible T-cell a-chemoattractant (1-TAC or CXCL11) recognize a single CXC chemokine receptor (CXCR), namely CXCR3 (5-8). IP-10, Mig and I-TAC attract monocytes and activated memory Thl, but not Th2, lymphocytes (9-11). Furthermore, eosinophils and subclasses of B and NK cells have been reported to express CXCR3 (11-12).

In addition to their role in leukocyte migration, chemokines play a role in angiogenesis (13-16). ELRCXC chemokines have been reported to be angiogenic due to their interaction with CXCR2 (17-18). In contrast, the non-ELR-CXC chemokines IP-10, Mig and PF-4 (platelet factor-4 or CXCL4) were found to have angiostatic activity but the molecular mechanism is not understood (15).

The NH2-terminal region of most chemokines is crucial for receptor binding and signaling activities. Some chemokines become chemotactic only when processed at the NH2-terminus, e. g. truncation of platelet basic protein (PBP) into NAP-2 (CXCL7) (19). Others, e. g. the monocyte chemotactic proteins MCP-1 (CCL2), MCP-2 (CCL8) and MCP-3 (CCL7), loose their chemotactic activity when NH2-terminal amino acids are cleaved off (20,21).

Aminopeptidases or endopeptidases that process chemokines at the NH2-terminus play an important role in the up-or down-regulation of chemokine activities. One of these enzymes, the membrane-associated protease dipeptidyl peptidase IV (DPP IV, EC3.4. 14.5) is highly specific. It cleaves off dipeptides from polypeptides with a proline, alanine or hydroxyproline at the second position. DPP IV, which is expressed on fibroblasts, epithelial and endothelial cells, is identical to the lymphocyte surface glycoprotein and T-cell activation marker CD26 (22). The extracellular domain of CD26/DPP IV also exists as a soluble and proteolytically active form in plasma and in cerebrospinal and seminal fluids. CD26/DPP IV interacts with CD45 (a protein tyrosine phosphatase) and with adenosine deaminase, has costimulatory activity in T-cell immune responses, plays a role in immune processes such as allograft rejection, suppresses malignant transformation and has been implicated in the regulation of insulin secretion (22,23). Recently, CD26/DPP IV was found to cleave a number of chemokines, but not cytokines (24). Moreover, one of the chemokine receptors that has been identified as a Thl cell marker, in addition to CCR5, is CXCR3 (9-11). Here we report that CD26/DPP IV efficiently cleaved all three CXCR3 ligands and demonstrate that the protease differently effects the receptor signaling, lymphocyte chemotactic and anti-angiogenic activities of these chemokines.

Illustrative embodiment of the invention Example 1: General method examples la) Reagents and cell lines Recombinant human chemokines (IP-10, Mig and I-TAC) and recombinant human IL-2 were purchased from PeproTech (Rocky Hill, NJ). Natural IL-8 was purified to homogeneity from conditioned medium from monocytes and contained an equimolar mixture of IL-8 (1-77), IL-8 (5-77) and IL-8 (6-77) (19). Soluble natural human CD26/DPP IV, without membrane anchor and starting at amino acid Gly31, was obtained from total seminal plasma and purified to homogeneity by anion exchange followed by affinity chromatography on immobilized adenosine deaminase as described (28). Human CXCR3 was stably expressed in CHO-K1 cells (ATCC: CCL-61) and cultured in HAM'S F-12 medium (Biowhittaker Europe, Verviers, Belgium) supplemented with 10 % FBS, 1 mM sodium pyruvate and 400 g/ml geneticin.

7b) In vitro truncation of chemokines by CD26/DPP IV In order to obtain efficient truncation, chemokines were treated with soluble CD26/DPP IV for 18 h at 37 oc and were subjected to SDS-PAGE as previously described (29).

NH2-terminal truncation was verified by Edman degradation on a pulsed liquid phase 477A/120A protein sequencer after electroblotting 0.5 to 2 zu of processed chemokine from the gel to Problot membranes (Applied Biosystems, Foster City, CA). Alternatively, truncated chemokines were purified by C8 RP-HPLC on a Brownlee Aquapore RP-300

column (50 x 1 mm ; PerkinElmer, Norwalk, CT) and the average Mr was determined by electrospray ion trap mass spectrometry (Esquire-LC ; Bruker Daltonic, Bremen, Germany). To determine the time-course of the NHz-terminal truncation, IP-10, Mig and I-TAC (5 gm) were incubated with soluble CD26/DPP IV (250,25, 2.5 and 0.25 U/l) in 15 Al of 50 mM Tris buffer, pH 7.5, supplemented with 1 mM EDTA. The specificity of the reaction was checked by incubating the chemokines with Tris buffer alone. Samples (5 p. l) were withdrawn after 5,15 and 30 min, and the reaction was stopped by addition of trifluoroacetic acid (final concentration of 0.1 %). The samples were desalted on a C18 ZipTip (Millipore, Bedford, MA) and the relative amounts of the NH2-terminally truncated chemokines were determined by ion trap mass spectrometry. The time course of the truncation (shown in the

figures) was constructed by normalizing the incubation times, taken into account that 25 U/I is close to the normal serum concentrations of DPP IV (30).

1 Chemotaxis assays Peripheral blood mononuclear cells were purified from buffy coats from healthy volunteers as previously described (31). Mononuclear cells were stimulated with anti-CD3 antibodies (OKT3: ATCC CRL 8001) in RPMI 1640 (Biowhittaker Europe) with 10 % FBS for 2 days before use. Alternatively, mononuclear cells were cultured in RPMI 1640 with 10 % FBS and treated with PHA (2 g/ml) for 3 days, washed with RPMI 1640 and kept in culture for two to three weeks in RPMI 1640 supplemented with 10 % FBS and 50 U/ml IL-2 before use in the chemotaxis assay (7).

Lymphocyte chemotaxis was performed in Boyden microchambers (Neuro Probe, Cabin John, MD) with fibronectin-coated, polyvinylpyrrolidone-free polycarbonate membranes (5 Am pore size, Coming Separations Division, Acton, MA). Lymphocytes were suspended

in HBSS + 0. 5 % (v/v) human serum albumin (HSA) at 2x106 cells/ml and were allowed to migrate for 2 h at 37 oc. Before chemotaxis, CXCR3 transfected CHO cells were resuspended in HBSS + 0. 5 % HSA and diluted to 1. 5x10 cells/ml. Boyden chamber chemotaxis experiments were performed for 2 h at 37 OC with 8 Am pore size polyvinylpyrrolidone-free polycarbonate membranes. In order to study antagonization, truncated chemokines were added at inactive concentrations together with the active substance to the bottom well of the Boyden chambers. Cells that migrated through the membrane were stained with DiffQuick (Merck, Darmstadt, Germany) and counted microscopically in 10 oil immersion fields (500 x magnification). The chemotactic index was calculated as the number of cells migrated to the sample divided by the number of cells

spontaneously migrated to the sample dilution medium (HBSS + 0. 5 % HSA).

1 Calcium signaling and receptor binding assays Alterations in intracellular calcium concentration ([Ca2+]i) in response to chemokines were monitored by fluorescence spectrometry. Briefly, CXCR3 transfected cells were loaded with the fluorescent dye Fura-2-AM for 30 min at 37 oC as previously described (29). Cells were washed with buffer, kept at 4 C and preincubated for 10 min at 37 oC before use. [Ca2+]; were measured in an LS50B spectrofluorimeter (PerkinElmer) at a final cell concentration of 106/mil.

Competition for 125I-labeled I-TAC binding was measured on freshly isolated peripheral blood mononuclear cells or on CXCR3-transfected cells as described (32). Briefly, 2 x 106 cells were incubated for 2 h at 4 C with 0.06 nM 125II-TAC (Amersham Pharmacia Biotech, Uppsala, Sweden) and varying concentrations of unlabeled chemokine. Cells were centrifuged and washed three times with 2 ml of PBS supplemented with 2 % (w/v) BSA and the radioactivity present on the cells was measured in a gamma counter. le) e) vivo testfor anti-angiogenic activity of chemokines Chemokines were tested for their angiogenic or angiostatic activity in the rabbit cornea micropocket model (16). Briefly, 32 mg sucralfate (Merck) were dissolved in 72 l PBS. Four III of this sucralfate solution was mixed with 4 l Hydron solution (12 % Hydron in ethanol ; Interferon Sciences, New Brunswick, NJ) and 5 iLl pellets were allowed to dry under UV light for 20 minutes. Subsequently, different concentrations of chemokines or dilution buffer (negative control) were dried on the pellets. One pellet was implanted 1 mm from the limbus into a corneal micropocket of each eye of an anesthetized New Zealand White rabbit. Neovascularization of the cornea was scored daily from day 5 to day 8 after implantation of the pellet. Maximal neovascularization was obtained between day 5 and 7. This maximal neovascularization was used for comparison.

Example 2: Processing of CXCR3 ligands by CD26/DPP IV Incubation of IP-10, I-TAC and Mig with CD26/DPP IV resulted in the effective removal ( > 95 %) of the NH2-terminal dipeptide (Table 1). After 18 h of incubation with CD26/DPP IV, no remaining intact chemokine was detectable by Edman degradation. Proteolysis beyond the penultimate proline was not observed confirming the purity and specificity of CD26/DPP IV. For biological evaluation, proteolytically cleaved CXCR3-ligands were purified by C-8 RP-HPLC. The purity and Mr of the cleaved chemokines were confirmed by mass spectrometry, which excludes carboxyterminal or internal processing (Table 1).

Mass spectrometry was used to study the time course of chemokine processing (Fig. 1).

When 5 juM IP-10 was incubated with serum concentrations (25 U/l) of CD26/DPP IV, 50 % of the chemokine was truncated within the first 5 min and after 20 min less than 10 % of the IP-10 proteins remained intact. In contrast, the kinetics of Mig processing were about 4 times slower (50 % conversion in 20 min) than that of IP-10, whereas 50 % and 90 % of intact I-TAC was converted into I-TAC (3-73) within 1.5 and 6 min, respectively. Thus, CD26/DPP IV processed I-TAC more than 10-fold faster than Mig.

Example 3 : Impaired lymphocy e chemotactic activity of IP-70 after CD26/DPP IV cleavage The chemotactic activity of intact and CD26/DPP IV-truncated IP-10 was compared on activated (by PHA or anti-CD3) lymphocytes. On PHA-stimulated lymphocytes (Fig. 2A), a dose-dependent chemotactic effect was obtained with intact IP-10 from 1 nM onward, whereas IP-10 (3-77) was still inactive at concentrations as high as 10 nM. Thus, processing of IP-10 by CD26/DPP IV resulted in a 30-fold reduction in lymphocyte chemotactic activity.

Similar results were observed with anti-CD3-activated lymphocytes (Fig. 2B), although these cells were less sensitive to IP-10-induced chemotaxis than PHA-stimulated lymphocytes. This observation correlates with the reported lower expression of CXCR3 on anti-CD3- stimulated cells (5).

Example 4 : Effect of CD26IDPP IV on the chemotactic and calcium signaling capacities of IP-10, Mig and I-TAC on CXCR3-transfected cells Only one receptor for IP-10, i. e. CXCR3, has been identified (6). Therefore, the chemotactic potencies of IP-10 and IP-10 (3-77) were compared on CHO cells transfected with CXCR3 (Fig. 3). For IP-10, a dose-dependent (minimal effective concentration of 3 nM) chemotactic response was observed, whereas for CD26/DPP IV-truncated IP-10 (3-77) the minimal effective concentration (loon) was 30-fold higher. Intact Mig and I-TAC induced a significant (p < 0. 01) chemotactic response on CXCR3-transfected cells at 10 nM. In contrast, truncated Mig (3-103) and I-TAC (3-73) were inactive at concentrations as high as 100 nM and 25 nM, respectively. Although their NH2-terminal amino acid is different, removal of two amino acids (including the penultimate proline) resulted in a similarly impaired chemotactic activity of all three CXCR3 agonists.

Furthermore, it was observed that intact IP-10 and I-TAC induced a significant increase in [Ca] i at concentrations higher than 1 nM, whereas 10-fold higher concentrations of Mig were required to obtain a detectable calcium response in CXCR3-transfected CHO cells (Fig. 3). Both IP-10 and I-TAC, truncated by CD26/DPP IV, lacked the calcium-signaling capacity through CXCR3. Indeed, although intact IP-10 induced calcium mobilization at 1 nM, IP-10 (3-77) was inactive in the calcium assay at concentrations as high as 60 nM. In contrast, no significant reduction in the weak calcium mobilizing capacity of Mig through CXCR3 could be observed for Mig (3-103). In conclusion, although none of the three ligands possessed CXCR3-mediated chemotactic activity after NHz-terminal truncation by CD26/DPPIV, Mig, but not IP-10 or I-TAC, retained its rather weak calcium signaling potency through CXCR3.

Example 6 : Binding properties of intact and CD26/DPP IV-truncated CXCR3-ligands The capacity of truncated IP-10 to compete for binding of 1251-labeled intact I-TAC to peripheral blood-derived mononuclear cells was significantly decreased (Fig. 4). At 100 nM IP-10 (3-77) displaced only 49 % of the labeled I-TAC from the cells, whereas the displacement with 100 nM intact IP-10 was 88 %. A comparable difference in binding competition capacity with 125I-Iabeled I-TAC to mononuclear cells was observed between intact and truncated 1-TAC. On CXCR3-transfected cells, the binding affinity of IP-10 (3-77) and I-TAC (3-73) was also clearly reduced (Fig. 4). No significant difference in binding competition capacity to mononuclear cells was observed between both Mig forms although there was a tendency for reduced binding potency for the truncated Mig. In addition, intact and truncated Mig could not significantly compete for 125I-I-TAC binding to CXCR3transfected cells.

Example 7: Anti-inflammatory effect of truncated IP-10(3-77) Since IP-10(3-77) failed to induce calcium mobilization and chemotactic responses, but retained some receptor binding properties, this truncated chemokine was tested as an inhibitor in chemotaxis assays on CXCR3-transfected cells. Addition of an inactive concentration IP-10 (3-77) (30 nM) to the lower well of the Boyden chamber resulted in a more than 50 % reduction in chemotactic response towards 30 nM of intact IP-10 and a complete inhibition of the chemotactic response towards 10 nM intact IP-10 (Fig. 5).

Example 8 : Anti-angiogenic activities oj IP-l processed by CD26/DPP IV When pellets containing 3 ng of natural human IL-8 were implanted into corneal micropockets on rabbit eyes, the induced neovascularization was maximal between day 5 and 7. Maximal inhibition of the angiogenic effect of IL-8 (3 ng) was obtained by addition of 10 ng of intact IP-10 (Fig. 6). Similarly, CD26-truncated IP-10 (3-77) equally inhibited IL-8induced angiogenesis. At 10 and 30 ng per pellet, no significant differences (Mann-Whitney U-test, p > 0.2) between the anti-angiogenic activities of intact and truncated IP-10 were observed. Although there was a tendency for intact IP-10 to be somewhat more efficient, these data indicate that in contrast to the inflammatory effect, the angiostatic potential of IP-10 is either not mediated through CXCR3 or implicates alternative CXCR3-triggered signal transduction pathways different from calcium signaling and chemotactic activity.

Discussion During the past two decades, more than 50 human chemokines have been identified.

Chemokines and chemokine receptors form a complex network that controls leukocyte migration during normal cell homing as well as inflammatory processes. The receptor specificity of chemokines determines the pattern of target cells. The expression of chemokines and chemokine receptors is regulated at the transcriptional level by different inducers. IFN-y, a prototypic Thl cytokine, has been reported to enhance the production of the non-ELR-CXC chemokines IP-10, Mig and I-TAC (7,33-36) but down-regulates the production of neutrophil chemotactic chemokines such as the ELRCXC chemokines IL-8 and ENA-78 (37). All three IFN-inducible non-ELR-CXC chemokines interact with CXCR3.

This receptor is highly expressed on activated memory T cells (CD45RO+ cells) and has been detected on a small portion of B cells and NK cells (5,11). Higher CXCR3 expression levels were detected on Thl lymphocytes compared to Th2 cells and IP-10 has been reported to attract Th 1 but not Th2 cells (9). Additional regulatory mechanisms of chemokine activity and receptor specificity were shown at the posttranscriptional level, mainly by NH2-terminal chemokine processing. For example, limited NH2-terminal truncation of IL-8 (e. g. by gelatinase B) enhanced its chemotactic potency whereas platelet basic protein was found to become chemotactic only when truncated into NAP-2 (19,38). In contrast, small modifications of the NH2-terminal residues of MCP-1, MCP-2 and MCP-3 decreased the biological activity of these CC chemokines (20,21).

Recently, the highly specific protease CD26/DPP IV has been reported to NH2-terminally process a number of chemokines with different effects on their chemotactic and antiviral activities (22,24). CD26 is expressed on a wide variety of cells including fibroblasts, epithelial and endothelial cells. Moreover, CD26 expression on CD45RO+ Thl lymphocytes is further increased upon activation. This protease cleaves GCP-2, SDF-1, RANTES, eotaxin, macrophage-derived chemokine (MDC), and the macrophage inflammatory protein-la (MIP-la) isoform LD78J3 into NH2-terminally truncated forms (22,24). Although MCP-1, MCP-2, MCP-3 and MIP-1J3 also possess the prerequisite penultimate proline, they are not processed by CD26/DPP IV. MCPs are protected from the proteolytic activity by their NH2terminal pyroglutamic acid (39). Here we report that CD26/DPP IV is able to efficiently cleave the NH2-terminal dipeptide of all three CXCR3 ligands IP-10, Mig and I-TAC. Timecourse experiments showed that under physiological serum concentrations of CD26/DPP IV, the majority of the chemokine is processed within a few minutes even when high concentrations of chemokine (5 its) are present. This rapid processing of the CXCR3 ligands by CD26/DPP IV at physiological concentrations is indicative for an important functional role of this interaction between this ubiquitous protease and these chemokines.

The chemotactic potency of the CXC chemokine SDF-1 and of the CC chemokines RANTES, eotaxin and MDC was drastically reduced upon NH2-terminal truncation by CD26/DPP IV (24). Accordingly, the binding affinity of truncated SDF-1, eotaxin and MDC for their respective receptors CXCR4, CCR3 and CCR4 decreased. In contrast, treatment of the CC-chemokine LD78P with CD26/DPP IV generated a highly potent monocyte and lymphocyte chemoattractant that retained strong anti-HIV-1 activity (40). The limited NH2-terminaI processing of IP-10 by CD26/DPP IV described here resulted in 30-fold reduced lymphocyte chemotactic activity. All three truncated CXCR3 ligands, i. e. IP-10, Mig and 1-TAC, lost chemotactic activity mediated through CXCR3. The calcium signaling capacity of IP-10 and I-TAC through CXCR3 was also abolished upon NH2-terminal truncation. CD26/DPP IV truncated IP-10 and 1-TAC retained weak CXCR3 binding properties, but higher doses of the truncated variant versus intact chemokine were necessary to remove 125I-I-TAC from its receptor. Finally, on CXCR3 transfected cells, truncated IP-10 acted as a chemotaxis inhibitor for the intact chemokine. So far, no information was available on the role of CXCR3 in the angiostatic activity of IP-10. CD26/DPP IV-truncated IP-10 retained anti-angiogenic activity in the rabbit cornea micropocket model. Thus, CXCR3-mediated chemotactic activity and calcium signaling are no prerequisites for the angiostatic activity of IP-10. Since CD26/DPP IV and CXCR3 expression and IFN-y production (the main inducer of the CXCR3 ligands) are hallmarks for activated memory Thl cells, processing of the three chemokines by CD26/DPP IV may constitute an important physiological regulatory mechanism during the attraction of leukocytes in case of a typical Thl response. Indeed in the case of a typical Thl response, it is crucial to dampen the cellular reaction after sufficient cell influx has occurred. Chemoattracted Thl lymphocytes, laden with CD26/DPP IV, cleave the angiostatic chemokines IP10, Mig and I-TAC resulting in down-modulation of their chemotactic activity. If the angiostatic effects would also be reduced, then the balance would be in favor of angiogenesis which may bring the local vessels and the circulating leukocytes closer to the Thl response site. Obviously, the latter effect does not take place and angiostasis thus helps to keep the circulating lymphocytes out of the field of attraction and to halt the inflammation. As a consequence, the interaction between CD26/DPP IV and CXCR3-ligands may play an important role in disease processes such as multiple sclerosis (41-43), B-cell leukemia (44), hepatocellular carcinoma (45), and skin diseases (34,25-26) where expression of CXCR3 and its ligands have been reported.

Figure legends Figure 1: Time course of the truncation of CXCR-3 ligands by CD26/DPP IV IP-10 (diamonds), Mig (circles) and I-TAC (triangles) at 5 AM were incubated for different time intervals with 25 U/l soluble CD26/DPP IV as indicated in the Methods section. The remaining concentration of the intact CXCR3-ligands determined by mass spectrometry is indicated in the graphs.

Figure 2: Effect of CD26/DPP IV on the lymphocyte chemotactic activity of IP-10 The chemotactic activity of intact (filled diamonds) and CD26/DPP IV-truncated (open diamonds) IP-10 for PHA- (panel A) or anti-CD3-stimulated (panel B) lymphocytes was measured in the Boyden chamber assay. Results represent the mean ( SEM) chemotactic index of 4 or more independent experiments with cells from different donors. The Student t-test was used for statistical analysis (** = p < 0. 05 ; *** = p < 0. 01 for a significantly positive chemotactic response compared to buffer controls).

Figure 3: Comparison of the chemotactic activity and calcium mobilizing capacity of

intact and truncated CXCR3 ligands on CXCR3-transfected cells. Intact (filled symbols) and CD26/DPP IV-truncated (open symbols) IP-10 (diamonds), Mig (circles) and I-TAC (triangles) were tested for their ability to induce a chemotactic response

or to increase the [Ca 2+], in CXCR3-transfected CHO cells. Results represent the mean ( SEM) chemotactic index of 4 or more independent experiments and the mean ( SEM) increase of the [Ca] i of 5 or more independent experiments. The detection limit for the increase of the [Ca2'], is indicated by the dashed line. The Student t-test was used for statistical analysis (* = p < 0.1 ; ** = p < 0. 05 ; *** = p < 0. 01 for a positive chemotactic response compared to buffer controls).

Figure 4: Receptor binding properties of intact and CD26/DPP IV-truncated CXCR3 agonists Intact (filled symbols) and CD26/DPP IV-truncated (open symbols) IP-10 (diamonds), I-TAC (triangles) and Mig (circles) were tested for their ability to compete for 11-labeled I-TAC binding to peripheral blood-derived mononuclear cells or CXCR3-transfected CHO cells.

Results represent the mean ( SEM) % of'I-1-TAC that binds to the cells compared to the amount of labeled I-TAC that binds the cells without addition of cold ligands (3 or more independent experiments). The Student t-test was used for statistical analysis (** = p < 0. 05; *** = p < O. OI for a significant difference between the intact and truncated chemokines).

Figure 5: Inhibition of chemotaxis by truncated IP-10 (3-77) The antagonistic activity ofCD26/DPP IV-truncated IP-10 was tested on CXCR3-transfected cells in the Boyden microchamber. Intact IP-10 (at 10 or 30 nM) was added with (black histograms) or without (open histograms) 30 nM IP-10 (3-77) in the bottom well of the chemotaxis chamber. Results represent the mean ( SEM) chemotactic index (C. I.) from 5

independent experiments. The Student t-test was used for statistical analysis (** = p < 0. 05 ; *** = p < 0. 01).

Figure 6 : Comparison of the anti-angiogenic activity of IP-10 and IP-10 (3-77) Hydron pellets were implanted in a corneal micropocket on a rabbit eye. Hydron pellets contained dilution buffer (panel A), 3 ng of natural human IL-8 (panel B), 3 ng of natural human IL-8 and 10 ng of intact IP-10 (1-77) (panel C), or 3 ng of natural human IL-8 and 10 ng of IP-10 (3-77) (panel D). Neovascularization was scored daily (score from 0 to 4) from day 4 to 8 and the maximal neovascularization (always occurring between day 5 and 7) is shown in panel E (each dot represents an independent experiment). The mean values are indicated by the lines and statistical analysis (comparison with the positive control that contained 3 ng IL-8) was performed using the Mann-Whitney U-test. The photographs in panels A to D are representative examples for the mean neovascularization scores as indicated in panel E.

Tables Table 1: NH2-terminal sequence analysis and mass spectrometry of CD26/DPP IV- treated chemokines.

Chemokine NH2-terminal sequence Reduction in Mr

Untreated CD26/DPP IV treated theoretical observed IP-10 VPLSRTVRCTC LSRTVRCTC 196. 25 196. 9 0. 4 Mig TPVVRKGRCSC VVRKGRCSC 198. 22 197. 7 0. 4 I-TAC FPMFKRGRCLC MFKRGRCLC 244.29 243.9 0. 3 A The NH2-terminal sequence of untreated and CD26/DPP IV treated (18 h incubation) chemokines was determined by automated Edman degradation.

B The average ( SD) difference between the Mr of intact and truncated CXCR3 ligands was calculated from mass measurements on 4 different incubations (between 2 and 30 min) with CD26/DPP IV.

Summary of the invention This invention involves rapid enzymatic processing of three interferon-induced CXC chemokines that bind to the same receptor and Thl lymphocyte marker CXCR3. The clipping enzyme is another Thl marker CD26/dipeptidyl peptidase IV (DPP IV). The reduction in signaling and chemotactic potency of the chemokines IP-10, Mig and I-TAC, after proteolytic processing by CD26/DPP IV, is demonstrative for a natural negative feedback mechanism in the inflammatory response. This includes inactivation of the CXCR3 ligands as chemoattractants, whilst retaining competition with intact chemokine for receptor binding. In contrast, the anti-angiogenic potential of IP-10 remains unaffected by the clipping. This indicates that the molecular mechanisms of action that underlie the inflammatory and antiangiogenic activities of these chemokines are different and that the angiostatic activity does not necessarily imply an activation of the IP-10 receptor CXCR3.

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Claims (1)

1. CLAIMS Claim 1: A method of truncating CXC chemokines, characterised in that said chemokines are exposed to or treated with a lymphocyte membrane protease, or variants, homologues or fragments of said lymphocyte membrane protease and said truncated chemokines have reduced CXCR3 binding properties, loss of calcium signalling capacity through CXCR3, a reduced chemotactic potency and said truncated chemokines have retained ability to inhibit angiogenic activity of cells.

   Claim 2: The method of claim 1, characterised in that said lymphocyte membrane protease is a CD26/dipeptidyl peptidase IV or variants, homologues or fragments thereof.

   Claim 3: The method of claim 2, characterised in that the chemokines were treated at least during 5 hours and at least at 15 oC with CD26/dipeptidyl peptidase IV or to variants, homologues or fragments thereof.

   Claim 4: The method of claim 1 to 3, characterised in that said chemokines are CXC chemokines.

   Claim 5: The method of claim 1 to 3, characterised in that the chemokines are of the group consisting of interferon (IFN) -inducible chemokines, monokine induced by IFN-y (Mig) and IFN-inducible T-cell a-chemoattractant (1-TAC).

   Claim 6: The method of claim 1 to 3, characterised in that the chemokine is IFN-y inducible protein-10 (IP-10).

   Claim 7: Truncated CXC chemokines or their variants, homologues or fragments having reduced CXCR3 binding properties, loss of calcium signalling capacity through CXCR3 and reduced chemotactic potency and said truncated chemokines having retained ability to inhibit angiogenic activity of cells, said chemokines being obtainable by any of the processing method of claim 1 to 6, said processed chemokine.

   Claim 8: The CXC chemokines of claim 7 for use in a treatment of multiple sclerosis.

   Claim 9: The CXC chemokines of claim 7 for use in treatment of cancer.

   Claim 10: The CXC chemokines of claim 7 for use in a treatment of B-cell leukemia.

   Claim 11: The CXC chemokines of claim 7 for use in a treatment of hepatocellular carcinoma.

   Claim 12: The CXC chimokinases of claim 7 for use in a treatment of skin diseases.