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GB2362212A - Method for the identification and speciation of bacteria of the burkholderia cepacia complex - Google Patents

Method for the identification and speciation of bacteria of the burkholderia cepacia complex

Info

Publication number
GB2362212A
GB2362212A GB0104647A GB0104647A GB2362212A GB 2362212 A GB2362212 A GB 2362212A GB 0104647 A GB0104647 A GB 0104647A GB 0104647 A GB0104647 A GB 0104647A GB 2362212 A GB2362212 A GB 2362212A
Authority
GB
United Kingdom
Prior art keywords
bacteria
burkholderia cepacia
complex
sequence information
nucleotide sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0104647A
Other versions
GB0104647D0 (en
Inventor
Eshwar Mahenthiralingam
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of British Columbia
Original Assignee
University of British Columbia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of British Columbia filed Critical University of British Columbia
Publication of GB0104647D0 publication Critical patent/GB0104647D0/en
Publication of GB2362212A publication Critical patent/GB2362212A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Identification and speciation of bacteria of the <I>Burkholderia cepacia</I> complex in a sample can be accomplished by: a) obtaining nucleotide sequence information for the <I>recA</I> gene in bacteria of the <I>Burkholderia cepacia</I> complex found in the sample; and b) comparing the nucleotide sequence information obtained for the <I>recA</I> gene in bacteria of the <I>Burkholderia cepacia</I> complex found in the sample with a standard library of nucleotide sequence information comprising standard nucleotide sequence information for at least three species of bacteria of the <I>Burkholderia cepacia</I> complex. Preferably, the nucleotide sequence information is obtained by evaluation of restriction fragment length polymorphism (RFLP). Other techniques for obtaining sequence information can also be used, including base-by-base determination of the sequence of the region of interest, sequence-specific oligonucleotide hybridization probes, and ligation techniques. Universal primers which can be used for amplification of all known members of the <I>Burkholderia cepacia</I> complex, and genomovar-specific primers which can be used for selective amplification of the <I>recA</I> gene from bacteria of one genomovar provide alternative analytical modalities. Speciation of bacteria of the <I>Burkholderia cepacia</I> complex can be used as a basis for administration of a vaccine specific to the flagellin of the bacteria, since it is shown that this flagellin is conserved across members of genomovar III, subgroup RG-B.
GB0104647A 1998-09-03 1999-09-03 Method for the identification and speciation of bacteria of the burkholderia cepacia complex Withdrawn GB2362212A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9911598P 1998-09-03 1998-09-03
US9911698P 1998-09-03 1998-09-03
PCT/CA1999/000813 WO2000014274A1 (en) 1998-09-03 1999-09-03 Method for the identification and speciation of bacteria of the burkholderia cepacia complex

Publications (2)

Publication Number Publication Date
GB0104647D0 GB0104647D0 (en) 2001-04-11
GB2362212A true GB2362212A (en) 2001-11-14

Family

ID=26795589

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0104647A Withdrawn GB2362212A (en) 1998-09-03 1999-09-03 Method for the identification and speciation of bacteria of the burkholderia cepacia complex

Country Status (3)

Country Link
AU (1) AU5612099A (en)
GB (1) GB2362212A (en)
WO (1) WO2000014274A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020055101A1 (en) 1995-09-11 2002-05-09 Michel G. Bergeron Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US20030049636A1 (en) 1999-05-03 2003-03-13 Bergeron Michel G. Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
US20100267012A1 (en) 1997-11-04 2010-10-21 Bergeron Michel G Highly conserved genes and their use to generate probes and primers for detection of microorganisms
CA2905326C (en) 1999-09-28 2016-09-27 Geneohm Sciences Canada Inc. Nucleic acids and methods for the detection of klebsiella
CN100432228C (en) * 2003-11-21 2008-11-12 广东省微生物研究所 Ribotide sequence of 2-naphthanic acid degradation bacteria DNA segment and its preparation method and application
CN116064866B (en) * 2022-09-23 2023-09-29 中国食品药品检定研究院 Kits, primers and methods for detection of Burkholderia cepacia and applications
CN117265140B (en) * 2023-05-05 2024-08-27 浙江省食品药品检验研究院 Burkholderia cepacia complex PCR detection primer and detection method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997001647A2 (en) * 1995-06-28 1997-01-16 Trustees Of Health & Hospitals Of The City Of Boston, Inc. Dna sequences for identifying highly transmissible lineages of pseudomonas (burkholderia) cepacia
WO1998020157A2 (en) * 1996-11-04 1998-05-14 Infectio Diagnostic (I.D.I.) Inc. Species-specific, genus-specific and universal dna probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997001647A2 (en) * 1995-06-28 1997-01-16 Trustees Of Health & Hospitals Of The City Of Boston, Inc. Dna sequences for identifying highly transmissible lineages of pseudomonas (burkholderia) cepacia
WO1998020157A2 (en) * 1996-11-04 1998-05-14 Infectio Diagnostic (I.D.I.) Inc. Species-specific, genus-specific and universal dna probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
INT J SYSTEMIC BACTERIOL, 1997, 47, 1188-1200, P VANDAMME ETAL *
J BACTERIOLOGY, 1995, 177, 6881-6893, S KARLIN ET AL *
J BACTERIOLOGY, 1998, 180, 1110-1118, BA HALES ET AL *
J CLINICAL MICROBIOL, 1994, 32, 2422-2424, SE DASEN ET AL *
J CLINICAL MICROBIOL, 1995, 33, 3304-3307, P YUK-FONG LIU ETAL *
J CLINICAL MICROBIOL, 1996, 34, 2914-2920, E MAHENTHIRALINGAM ET AL *
J MED MICROBIOL, 1995, 43, 436-441, HC RYLEY ET AL *
PEDIATRIC RES, 35, 394A, JJ LIPUMA ET AL *

Also Published As

Publication number Publication date
GB0104647D0 (en) 2001-04-11
WO2000014274A1 (en) 2000-03-16
AU5612099A (en) 2000-03-27

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Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)