GB2289945A - Microbiological test method and reagents - Google Patents
Microbiological test method and reagentsInfo
- Publication number
- GB2289945A GB2289945A GB9515786A GB9515786A GB2289945A GB 2289945 A GB2289945 A GB 2289945A GB 9515786 A GB9515786 A GB 9515786A GB 9515786 A GB9515786 A GB 9515786A GB 2289945 A GB2289945 A GB 2289945A
- Authority
- GB
- United Kingdom
- Prior art keywords
- amount
- reagents
- adp
- test method
- luciferase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title abstract 4
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 2
- 238000012009 microbiological test Methods 0.000 title 1
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 abstract 3
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 abstract 3
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 abstract 2
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 abstract 2
- 108060001084 Luciferase Proteins 0.000 abstract 2
- 239000005089 Luciferase Substances 0.000 abstract 2
- 230000003834 intracellular effect Effects 0.000 abstract 2
- 239000000463 material Substances 0.000 abstract 2
- 102000002281 Adenylate kinase Human genes 0.000 abstract 1
- 108020000543 Adenylate kinase Proteins 0.000 abstract 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 abstract 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 abstract 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 abstract 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 abstract 1
- 108091000080 Phosphotransferase Proteins 0.000 abstract 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 238000003149 assay kit Methods 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 102000020233 phosphotransferase Human genes 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2304/00—Chemical means of detecting microorganisms
- C12Q2304/60—Chemiluminescent detection using ATP-luciferin-luciferase system
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A method for determining the presence and/or amount of microorganisms and/or their intracellular material present in a sample comrpising estimating the amount of adenylate kinase therein by its ability to convert adenosine diphosphate (ADP) to adenosine triphosphate (ATP) and relating that to the presence/or amount of organism and/or intracellular material. The method provides improved sensitivity over existing luciferase/luciferin assays. Reagents including purified ADP and adenyl kinase free luciferase are provided together with test kits including these and apparatus for automated operation of the method. <IMAGE>
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9515786A GB2289945B (en) | 1993-01-21 | 1995-07-27 | Detecting microorganisms by assaying for ATP produced by intracellular adenylate kinase |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB939301118A GB9301118D0 (en) | 1993-01-21 | 1993-01-21 | Enzyme linked assays |
| PCT/GB1994/000118 WO1994017202A1 (en) | 1993-01-21 | 1994-01-21 | Microbiological test method and reagents |
| GB9515786A GB2289945B (en) | 1993-01-21 | 1995-07-27 | Detecting microorganisms by assaying for ATP produced by intracellular adenylate kinase |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| GB9515786D0 GB9515786D0 (en) | 1995-10-04 |
| GB2289945A true GB2289945A (en) | 1995-12-06 |
| GB2289945B GB2289945B (en) | 1997-06-18 |
Family
ID=26302319
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB9515786A Expired - Fee Related GB2289945B (en) | 1993-01-21 | 1995-07-27 | Detecting microorganisms by assaying for ATP produced by intracellular adenylate kinase |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB2289945B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3933592A (en) * | 1965-02-17 | 1976-01-20 | Hazleton Laboratories, Incorporated | Method of detecting living microorganisms |
| EP0054676A2 (en) * | 1980-12-18 | 1982-06-30 | Roche Diagnostics GmbH | Process for determining HL antigens |
| EP0238352A2 (en) * | 1986-03-21 | 1987-09-23 | Minnesota Mining And Manufacturing Company | Method for the selective detection of microbial nucleotides |
-
1995
- 1995-07-27 GB GB9515786A patent/GB2289945B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3933592A (en) * | 1965-02-17 | 1976-01-20 | Hazleton Laboratories, Incorporated | Method of detecting living microorganisms |
| EP0054676A2 (en) * | 1980-12-18 | 1982-06-30 | Roche Diagnostics GmbH | Process for determining HL antigens |
| EP0238352A2 (en) * | 1986-03-21 | 1987-09-23 | Minnesota Mining And Manufacturing Company | Method for the selective detection of microbial nucleotides |
Non-Patent Citations (3)
| Title |
|---|
| Adv. Biotechnol. (Proc. Int. Ferment. Symp. 6th 1980) Vol. 1, 1981 pages 457-462 * |
| Chemical Abstracts, Vol. 96, No. 21, 1982, page 350 * |
| Journal of Biochemical and Biophysical Methods Vol. 1, No. 31979, pages 163-169 * |
Also Published As
| Publication number | Publication date |
|---|---|
| GB2289945B (en) | 1997-06-18 |
| GB9515786D0 (en) | 1995-10-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| HU9502220D0 (en) | Microbial test method and reagents | |
| GB2303919A (en) | Microbiological test method and reagents | |
| GB2304892A (en) | Capture assays | |
| CA2356317A1 (en) | Method and apparatus for measuring sample by luminescence | |
| DK1513878T3 (en) | Compositions and Methods for Analysis of Apo B48 and Apo B100 | |
| GB9911095D0 (en) | Microbiological test method and reagents | |
| GB2289945A (en) | Microbiological test method and reagents | |
| ATE125623T1 (en) | REDUCABLE INDICATORS CONTAINING PYROGALLOL AND THEIR USE. | |
| CA2512636A1 (en) | Assay methods and materials | |
| Pike et al. | Rapid and semi-automated microbiological analyses for water quality | |
| LeDoux et al. | Domoic acid: State of contamination of shellfish in France- preliminary study | |
| Karl | MICROBIAL BIOMASS, VIABILITY, METABOLISM, AND GROWTH |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 20000121 |