GB2189809A - Immobilized biological material - Google Patents
Immobilized biological material Download PDFInfo
- Publication number
- GB2189809A GB2189809A GB8610913A GB8610913A GB2189809A GB 2189809 A GB2189809 A GB 2189809A GB 8610913 A GB8610913 A GB 8610913A GB 8610913 A GB8610913 A GB 8610913A GB 2189809 A GB2189809 A GB 2189809A
- Authority
- GB
- United Kingdom
- Prior art keywords
- biological material
- biological
- fibre
- cells
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 239000012620 biological material Substances 0.000 title claims abstract description 37
- 102000004190 Enzymes Human genes 0.000 claims abstract description 11
- 108090000790 Enzymes Proteins 0.000 claims abstract description 11
- 239000000243 solution Substances 0.000 claims abstract description 11
- 239000007864 aqueous solution Substances 0.000 claims abstract description 5
- 239000004744 fabric Substances 0.000 claims abstract description 5
- 239000011942 biocatalyst Substances 0.000 claims abstract description 3
- 239000000835 fiber Substances 0.000 claims description 14
- 210000004027 cell Anatomy 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 6
- 239000000376 reactant Substances 0.000 claims description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 4
- 238000011143 downstream manufacturing Methods 0.000 claims description 4
- 230000003100 immobilizing effect Effects 0.000 claims description 4
- 229910010272 inorganic material Inorganic materials 0.000 claims description 4
- 239000011147 inorganic material Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- 239000011149 active material Substances 0.000 claims description 2
- 229940072056 alginate Drugs 0.000 claims description 2
- 235000010443 alginic acid Nutrition 0.000 claims description 2
- 229920000615 alginic acid Polymers 0.000 claims description 2
- 159000000007 calcium salts Chemical class 0.000 claims description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 2
- 239000001569 carbon dioxide Substances 0.000 claims description 2
- 239000003054 catalyst Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 239000012266 salt solution Substances 0.000 claims description 2
- 238000009751 slip forming Methods 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 210000005253 yeast cell Anatomy 0.000 claims description 2
- 239000003049 inorganic solvent Substances 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/26—Constructional details, e.g. recesses, hinges flexible
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Immunology (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Biological material is solubilized in an inorganic/organic solvent and spun into a thin continuous thread by using a nozzle to extrude the solution into a bath of an aqueous solution. The continuous thread containing the biological material is formed into a cloth or wound on to a mandrel to form a biological reactor or biocatalyst reactor. <IMAGE>
Description
SPECIFICATION
Immobilized biological material
This invention relates to methods of and apparatus for encapsulating or immobilizing cells, enzymes and other biological materials in such a from thatthe cells, enzymes and other biological materials are readily available to carry out reactions orto act as catalysts for other reactions.
Accordinglythe invention consists of biological materials held by on or in a continuouslyformed filamentorfibreoforganicand inorganic materials.
Cells, enzymes or other biological materials are solubilized in a solvent containing organicand inorganic materials. The solution is extruded through a jet into baths containing aqueous solutions that forms or spins the emerging jet of liquid into a continuousthin filament orfibre. The fibre contains the cells, enzymes or other biological materials in an encapsulated or immobilized form within and/or on thesurface ofthefilm.
The invention provides a very simple way of encapsulating biologically active material to form a very flexible design of biochemical reactor that holds the reactive or catalystic material separate from but in intimate contact with the reacting fluids.
One preferred form of the invention will now be described.
Asolutionofalginateandyeastcells 1 is spun into a thin continuous thread by using a nozzle 2 to extrude the solution into a bath containing an aqueous solution of a calcium salt 3 as shown in
Figure 1/2. The speed of emission ofthe alginate/yeast solution into the aqueous salt solutions is such that a thin thread or filament offibre 4 is formed which is capable of being wound onto a bobbin 5. The fibre can be woven into a cloth or wound around aformer to form a pad or bobbin of material.In this case the thread is wound into a mandrel that forms the heart of a biocatalyst/bioreactorsystem.The mandrel 6 with the th read wound onto its surface is placed in a housing 7 shown in Figures 2/2, such that a sugar solution 8 can be passed through the mass offibres which contain the yeast cells. Reactions are stimulated by the cells and proceeds to give alcohol, water and carbon dioxide. The outgoing solution containing these is taken on to downstream processing where the products are separated.
1. Immobilization of biological material by incorporating the biological material in a continuously spun fibre.
2. The fibre incorporating the biological material in Claim 1 can be woven into cloth orfabricorwound onto a mandrel to form a biochemical reactor in which the biological material is the active component.
3. Because the biological material is encapsulated within or on the fibre, it contacts the reactants passing to the biological reactor of Claim 2 but is not present in the products emerging from the reactor thus simplifying downstream processing of the products.
4. The cross-sectional area of the fibre is small compared to the surface area exposed to the reactant fluids ensuring that the maximum exposed area of the biological material is brought ipto intimate contact with the reactants even though the biological material is in stationary form.
5. Despite the availability of the biological material for reaction as expressed in Claim 4, the structure of the fibre is such that it holds the biological material by bonds so that elutriation of the biological material by the product fluids is avoided.
6. A method of immobilizing biological material as shown in Figure 1/2 to form a reactor, one type of which is shown in Figure 2/2.
**WARNING** end of DESC field may overlap start of CLMS **.
Claims (6)
1. Immobilization of biological material by incorporating the biological material in a continuously spun fibre.
2. The fibre incorporating the biological material in Claim 1 can be woven into cloth orfabricorwound onto a mandrel to form a biochemical reactor in which the biological material is the active component.
3. Because the biological material is encapsulated within or on the fibre, it contacts the reactants passing to the biological reactor of Claim 2 but is not present in the products emerging from the reactor thus simplifying downstream processing of the products.
4. The cross-sectional area of the fibre is small compared to the surface area exposed to the reactant fluids ensuring that the maximum exposed area of the biological material is brought ipto intimate contact with the reactants even though the biological material is in stationary form.
5. Despite the availability of the biological material for reaction as expressed in Claim 4, the structure of the fibre is such that it holds the biological material by bonds so that elutriation of the biological material by the product fluids is avoided.
6. A method of immobilizing biological material as shown in Figure 1/2 to form a reactor, one type of which is shown in Figure 2/2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB8610913A GB2189809A (en) | 1986-05-03 | 1986-05-03 | Immobilized biological material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB8610913A GB2189809A (en) | 1986-05-03 | 1986-05-03 | Immobilized biological material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB8610913D0 GB8610913D0 (en) | 1986-06-11 |
| GB2189809A true GB2189809A (en) | 1987-11-04 |
Family
ID=10597344
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB8610913A Withdrawn GB2189809A (en) | 1986-05-03 | 1986-05-03 | Immobilized biological material |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB2189809A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5158881A (en) * | 1987-11-17 | 1992-10-27 | Brown University Research Foundation | Method and system for encapsulating cells in a tubular extrudate in separate cell compartments |
| US5283187A (en) * | 1987-11-17 | 1994-02-01 | Brown University Research Foundation | Cell culture-containing tubular capsule produced by co-extrusion |
| EP0568940A3 (en) * | 1992-05-05 | 1995-04-05 | Interpharm Lab Ltd | Bioreactor with radial flow direction. |
| US5418154A (en) * | 1987-11-17 | 1995-05-23 | Brown University Research Foundation | Method of preparing elongated seamless capsules containing biological material |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1361963A (en) * | 1972-01-28 | 1974-07-30 | Snam Progetti | Process for producing fructose or a syrup containing fructose and glucose |
| GB1386674A (en) * | 1971-04-23 | 1975-03-12 | Snam Progetti | Process for the enzymatic production of l-tryptophan |
| GB1453744A (en) * | 1973-03-22 | 1976-10-27 | Snam Progetti | Permeable cellulosic bodies containing enzymes |
| GB1513169A (en) * | 1974-07-31 | 1978-06-07 | Snam Progetti | Fibres incorporating antibodies antigens and antisera method for their preparation and their use |
| GB2008966A (en) * | 1977-10-13 | 1979-06-13 | Snam Progetti | Radial flow reactor for use in catalyzed reactions |
| GB2041941A (en) * | 1979-02-15 | 1980-09-17 | Eni Ente Naz Idrocarb | Microporous Bodies having Occluded Agents |
| EP0125105A2 (en) * | 1983-05-09 | 1984-11-14 | Pfizer Inc. | Immobilization of catalytically active microorganisms in agar gel fibers |
| EP0173915A2 (en) * | 1984-09-07 | 1986-03-12 | Joachim Prof. Dr. Klein | Biocatalyst and process for preparing it |
-
1986
- 1986-05-03 GB GB8610913A patent/GB2189809A/en not_active Withdrawn
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1386674A (en) * | 1971-04-23 | 1975-03-12 | Snam Progetti | Process for the enzymatic production of l-tryptophan |
| GB1361963A (en) * | 1972-01-28 | 1974-07-30 | Snam Progetti | Process for producing fructose or a syrup containing fructose and glucose |
| GB1453744A (en) * | 1973-03-22 | 1976-10-27 | Snam Progetti | Permeable cellulosic bodies containing enzymes |
| GB1513169A (en) * | 1974-07-31 | 1978-06-07 | Snam Progetti | Fibres incorporating antibodies antigens and antisera method for their preparation and their use |
| GB2008966A (en) * | 1977-10-13 | 1979-06-13 | Snam Progetti | Radial flow reactor for use in catalyzed reactions |
| GB2041941A (en) * | 1979-02-15 | 1980-09-17 | Eni Ente Naz Idrocarb | Microporous Bodies having Occluded Agents |
| EP0125105A2 (en) * | 1983-05-09 | 1984-11-14 | Pfizer Inc. | Immobilization of catalytically active microorganisms in agar gel fibers |
| EP0173915A2 (en) * | 1984-09-07 | 1986-03-12 | Joachim Prof. Dr. Klein | Biocatalyst and process for preparing it |
Non-Patent Citations (1)
| Title |
|---|
| BIOTECHNOL. NEWSWATCH (MCGRAW-HILLS)VOL 4 ISSUE 20 P6-7 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5158881A (en) * | 1987-11-17 | 1992-10-27 | Brown University Research Foundation | Method and system for encapsulating cells in a tubular extrudate in separate cell compartments |
| US5283187A (en) * | 1987-11-17 | 1994-02-01 | Brown University Research Foundation | Cell culture-containing tubular capsule produced by co-extrusion |
| US5284761A (en) * | 1987-11-17 | 1994-02-08 | Brown University Research Foundation | Method of encapsulating cells in a tubular extrudate |
| US5389535A (en) * | 1987-11-17 | 1995-02-14 | Brown University Research Foundation | Method of encapsulating cells in a tubular extrudate |
| US5418154A (en) * | 1987-11-17 | 1995-05-23 | Brown University Research Foundation | Method of preparing elongated seamless capsules containing biological material |
| US5643773A (en) * | 1987-11-17 | 1997-07-01 | Brown University Research Foundation | Preparation of elongated seamless capsules containing a coaxial rod and biological material |
| EP0568940A3 (en) * | 1992-05-05 | 1995-04-05 | Interpharm Lab Ltd | Bioreactor with radial flow direction. |
| US5705390A (en) * | 1992-05-05 | 1998-01-06 | Interpharm Laboratories Ltd. | Bioreactor |
Also Published As
| Publication number | Publication date |
|---|---|
| GB8610913D0 (en) | 1986-06-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |