GB2188934A - Polypeptide production - Google Patents
Polypeptide productionInfo
- Publication number
- GB2188934A GB2188934A GB08710516A GB8710516A GB2188934A GB 2188934 A GB2188934 A GB 2188934A GB 08710516 A GB08710516 A GB 08710516A GB 8710516 A GB8710516 A GB 8710516A GB 2188934 A GB2188934 A GB 2188934A
- Authority
- GB
- United Kingdom
- Prior art keywords
- amino acid
- polypeptide
- polypetide
- cysteine
- cystine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 3
- 210000004899 c-terminal region Anatomy 0.000 abstract 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Natural products NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 abstract 2
- 239000004471 Glycine Substances 0.000 abstract 2
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 abstract 2
- 235000018417 cysteine Nutrition 0.000 abstract 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 abstract 2
- 229960003067 cystine Drugs 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 230000002862 amidating effect Effects 0.000 abstract 1
- 125000003368 amide group Chemical group 0.000 abstract 1
- 150000001408 amides Chemical class 0.000 abstract 1
- 125000004354 sulfur functional group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/17—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced ascorbate as one donor, and incorporation of one atom of oxygen (1.14.17)
- C12Y114/17003—Peptidylglycine monooxygenase (1.14.17.3)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
A process for the production of a polypeptide having a C-terminal amide group where the polypetide has one or more cysteine or cystine amino acid residues. A second polypetide comprising the amino acid sequence of the first polypeptide and a C-terminal glycine amino acid residue and where the cysteine or cystine sulphur group or groups is or are protected is reacted with an amidating enzyme capable of converting the C-terminal glycine amino acid residue to an amide.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB858523156A GB8523156D0 (en) | 1985-09-19 | 1985-09-19 | Polypeptide production |
| PCT/GB1986/000559 WO1987001729A1 (en) | 1985-09-19 | 1986-09-19 | Polypeptide production |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| GB8710516D0 GB8710516D0 (en) | 1987-06-03 |
| GB2188934A true GB2188934A (en) | 1987-10-14 |
| GB2188934B GB2188934B (en) | 1990-01-24 |
Family
ID=10585423
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB858523156A Pending GB8523156D0 (en) | 1985-09-19 | 1985-09-19 | Polypeptide production |
| GB8710516A Expired - Lifetime GB2188934B (en) | 1985-09-19 | 1986-09-19 | Polypeptide production using an amidating enzyme |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB858523156A Pending GB8523156D0 (en) | 1985-09-19 | 1985-09-19 | Polypeptide production |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0236416B1 (en) |
| JP (1) | JPS63501541A (en) |
| AT (1) | ATE78872T1 (en) |
| AU (1) | AU603553B2 (en) |
| DE (1) | DE3686257T2 (en) |
| DK (1) | DK250287D0 (en) |
| GB (2) | GB8523156D0 (en) |
| WO (1) | WO1987001729A1 (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6319685B1 (en) * | 1984-09-27 | 2001-11-20 | Unigene Laboratories, Inc. | Alpha-amidating enzyme compositions and processes for their production and use |
| JPS6437298A (en) * | 1987-08-04 | 1989-02-07 | Hiroshi Okamoto | Production of c-terminal amidated peptide |
| JP2653820B2 (en) * | 1988-03-14 | 1997-09-17 | 壽之 松尾 | Amidating enzyme and method for producing the same |
| AU637765B2 (en) * | 1988-05-30 | 1993-06-10 | Shiseido Company Ltd. | C-terminus amidation enzyme composition, process for its preparation and its use |
| US5153308A (en) * | 1988-06-16 | 1992-10-06 | Teijin Limited | S-sulfonated calcitonin derivatives |
| JP2535398B2 (en) * | 1989-01-17 | 1996-09-18 | サントリー株式会社 | Method for producing amidated peptide |
| GB9015369D0 (en) * | 1990-07-12 | 1990-08-29 | Erba Carlo Spa | Amidated fibrinolytic enzymes and their precursors,and processes for their preparation |
| JP5170976B2 (en) * | 2006-04-11 | 2013-03-27 | 株式会社イミュノフロンティア | Protein complex and method for producing the same |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0133282A1 (en) * | 1983-07-27 | 1985-02-20 | Hoechst Aktiengesellschaft | Preparation of acidamide-carboxy-terminal polypeptides |
| EP0134631A1 (en) * | 1983-06-15 | 1985-03-20 | Celltech Limited | Peptides, pharmaceutical compositions, genes, vectors, host organisms, processes for their production and diagnostic reagents |
-
1985
- 1985-09-19 GB GB858523156A patent/GB8523156D0/en active Pending
-
1986
- 1986-09-19 AT AT86905378T patent/ATE78872T1/en not_active IP Right Cessation
- 1986-09-19 JP JP61504817A patent/JPS63501541A/en active Pending
- 1986-09-19 DE DE8686905378T patent/DE3686257T2/en not_active Expired - Fee Related
- 1986-09-19 AU AU63761/86A patent/AU603553B2/en not_active Expired - Fee Related
- 1986-09-19 WO PCT/GB1986/000559 patent/WO1987001729A1/en not_active Ceased
- 1986-09-19 EP EP86905378A patent/EP0236416B1/en not_active Expired - Lifetime
- 1986-09-19 GB GB8710516A patent/GB2188934B/en not_active Expired - Lifetime
-
1987
- 1987-05-18 DK DK250287A patent/DK250287D0/en not_active Application Discontinuation
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0134631A1 (en) * | 1983-06-15 | 1985-03-20 | Celltech Limited | Peptides, pharmaceutical compositions, genes, vectors, host organisms, processes for their production and diagnostic reagents |
| EP0133282A1 (en) * | 1983-07-27 | 1985-02-20 | Hoechst Aktiengesellschaft | Preparation of acidamide-carboxy-terminal polypeptides |
Non-Patent Citations (2)
| Title |
|---|
| FEBS LETTERS, VOLUME 152, NO. 2, FEBRUARY 1983, PAGES 277-281 * |
| NATURE, VOLUME 298 12 AUGUST 1982, PAGES 686-688 * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0236416B1 (en) | 1992-07-29 |
| WO1987001729A1 (en) | 1987-03-26 |
| GB8523156D0 (en) | 1985-10-23 |
| DK250287A (en) | 1987-05-18 |
| AU6376186A (en) | 1987-04-07 |
| DE3686257T2 (en) | 1993-03-04 |
| ATE78872T1 (en) | 1992-08-15 |
| GB8710516D0 (en) | 1987-06-03 |
| JPS63501541A (en) | 1988-06-16 |
| GB2188934B (en) | 1990-01-24 |
| EP0236416A1 (en) | 1987-09-16 |
| DE3686257D1 (en) | 1992-09-03 |
| AU603553B2 (en) | 1990-11-22 |
| DK250287D0 (en) | 1987-05-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 19930919 |