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GB2035298A - Preparation of beta -lactam compounds - Google Patents

Preparation of beta -lactam compounds Download PDF

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Publication number
GB2035298A
GB2035298A GB7930152A GB7930152A GB2035298A GB 2035298 A GB2035298 A GB 2035298A GB 7930152 A GB7930152 A GB 7930152A GB 7930152 A GB7930152 A GB 7930152A GB 2035298 A GB2035298 A GB 2035298A
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Prior art keywords
ester
group
groups
optionally substituted
xis
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GB7930152A
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GB2035298B (en
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Beecham Group PLC
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Beecham Group PLC
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Priority to GB7930152A priority Critical patent/GB2035298B/en
Publication of GB2035298A publication Critical patent/GB2035298A/en
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Publication of GB2035298B publication Critical patent/GB2035298B/en
Expired legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D503/00Heterocyclic compounds containing 4-oxa-1-azabicyclo [3.2.0] heptane ring systems, i.e. compounds containing a ring system of the formula:, e.g. oxapenicillins, clavulanic acid derivatives; Such ring systems being further condensed, e.g. 2,3-condensed with an oxygen-, nitrogen- or sulfur-containing hetero ring

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Abstract

A process for the preparation of an ester of 9-mercaptodeoxyclavulanic acid comprises the reaction of potassium trithiocarbonate with the corresponding ester of a compound of the formula (I): <IMAGE> wherein X is a readily displaceable moiety.n

Description

SPECIFICATION Process for the preparation of p-lactam compounds The present invention relates to the preparation of esters of 9-mercaptodeoxyclavulanic acid derivatives.
Esters of 9-mercaptodeoxyclavulanic acid (also called 3R, 5R,Z-2-(2-mercaptoethylidene)- clavam-3-carboxylic acid) and their use are described in French Patent Specification No. 2342292. A Process has now been found for the preparation of said esters which gives better yields and which is more convenient to employ than the previously known process.
The present invention provides a process for the preparation of an ester of 9-mercaptodeoxyclavulanic acid which process comprises the reaction of potassium trithiocarbonate with the corresponding ester of a compound oftheformula (I):
wherein X is a readily displaceable moiety.
Suitable readily displaceable moieties include the chlorine or bromine atoms and the groups of the sub-formuiae (a) and (b): R1-O (a) OSO3 HNR2R3R4 (b) where R1 is an optionally substituted phenylacetyl group, a C35 acyl group optionally substituted by one or more chlorine atoms, or an optionally substituted mesyl or tosyl group; and R2, R3 and R4 are groups such that NR2R3R4 is a tertiary amine.
Suitable groups R1 include acetyl, chloroacetyl, dichloroacetyl, trichloroacetyl the like of which dichloroacetyl is preferred.
Suitable groups R2, R3 and R4 include C1-6 alkyl groups such as methyl, ethyl and like groups.
Suitable groups R1 include dichloroacetyl and the like.
Suitable groups R2, R3 and R4 include C-6 alkyl groups.
Most suitably an ester of 9-chlorodeoxy-clavulanic acid is employed.
The reaction is normally carried out in a non-hydroxylic medium such as dimethylformamide, hexamethyl-phosphoramide, tetrahydrofuran and the like organic solvent of relatively high dielectric constant.
It has been preferred to carry out this reaction at a slightly depressed or ambient temperature, for example -10 Cto +30"C although non-extreme temperatures outside this range may be employed if desired.
The desired ester may be obtained from the reaction mixture by neutralisation followed by evaporation.
However this method of recovery tends to produce an impure product so that it is desireable to dilute the reaction solution with a water imiscible organic solvent such as ethyl acetate prior to or after neutralisation and then recover the desired ester from this organic solution after it has been washed with water to remove the water-soluble impurities.
In general the organic solvent is removed by evaporation although other conventional methods may be employed if desired.
The product may be further purified chromatographically if thought desirable.
Since the compound prepared by the process of this invention will normally be employed as a chemical intermediate rather than a ss-lactamase inhibitor, it is preferred that the ester employed is one convertible to the free acid or its salt in mild manner. Suitable esters of this type include those described in the hereby incorporated French Patent specification No. 2342292 or Belgian Patent No. 850779 as suitable for 9-thioderivatives of deoxyclavulanic acid. Such esters are most aptly those which can be converted into corresponding acid or its salt by hydrogenation optionally in the presence of a base or those which can be converted into a salt of the corresponding acid by mild base hydrolysis. Such esters are described in the aforementioned Patents.These suitable esters include benzyl and substituted benzyl esters such as the p-methoxybenzyl, p-bromobenzyl, p-nitrobenzyl and the like or lower alkyl or alkoxyalkyl esters such as the methyl or methoxymethyl ester.
A particularly favoured ester is the p-nitrobenzyl ester since the product of the reaction is then a crystalline solid.
The following Example illustrates the invention.
EXAMPLE 4-Nitrobenzyl (3R, 5R, Z)-2-(2-mercapto eth ylldene)-cla vam-3-carboxylate
4-Nitrobenzyl (3R, 5R, Z)-2-(-chloroethylidene)-clavam-3-carboxylate (325.5 mg; 1 m.mole) was added i to a solution of dry dimethylformamide and potassium trithiocarbonate (232 mg; 1.25 m.mole) at 0-5 under nitrogen. The mixture was stirred for 2 min at 0-5 and then at room temperature for 10 min. after which time it was poured into a rapidly stirred solution of ethyl-acetate (90 ml), water (70 ml) in HCI (10 ml). (The operation carried out under nitrogen).The organic layer was separated and washed with water (4 x 50 ml) dried over anhydrous magnesium sulphate, and evaporated to give a yellow oil (0.3; 85%) which crystailised on standing in the fridge (5 ). Fecrystallisation from ethylacetate-Detrol (60-80 ) gave the title ester (260 mg) as pale yellow crystals, m.p.135-137 ; [&alpha;]D20 + 23.2 (C.0.34; CHCl3); Vmax (CHC13) 1803,1760,1525 and 1350 cm-1; b(DCCI3) 1.58 (1H,t,J7Hz, SH), 3.04 (1H, d, J 17 Hz,6ss-CH),3.21 (2H,t,J7 Hz, 9-CH2), 3.5 (1H, dd, d 17 Hz and 2.5 Hz, 6a-CH), 4.82 (1 H, t,J8 Hz, 8-CH), 5.1 (1H, s,3-CH),5.3 (2H, s, -CO2CH2), 5.69 (1H, d,J2.5 Jz, 5-CH), 7.5 and 8.21 (4H, 2d, aromatic-H).

Claims (10)

1. A process for the preparation of an ester of 9-mercaptodeoxyclavulanic acid which process comprises the reaction of potassium trithiocarbonate with the corresponding ester of a compound of the formula (I):
wherein Xis a readily displaceable moiety.
2. A process as claimed in claim 1 wherein X is a chlorine or bromine atom or groups of the subformulae (a) or (b):
wherein R1 is an optionally substituted phenyl acetyl group, a C26 acyl group optionally substituted by one or more chlorine atoms or an optionally substituted mesyl ortosyl group; and R2, R3 and R4 are groups such that NR2R3R4 is a tertiary amine.
3. A process as claimed in claim 2 wherein X is a group OR, where R is an acetyl, chloroacetyl, dichloroacetyl ortrichloroacetyl qrnuo.
4. A process as claimed in claim 2 wherein Xis a OSO3 - HNR2R3R4 group wherein R2, R3 and R4 are C1-6 alkyl groups.
5. A process as claimed in claim 1 wherein Xis chlorine.
6. A process as claimed in claims 1 to 5 which is carried out in a non-hydrolyic medium.
7. A process as claimed in claims 1 to 6 which is carried out at a temperature in the range -10Cto +30"C.
8. A process as claimed in any of claims 1 to 7 which comprises reacting a benzyl ester, a substituted benzyl ester, a lower alkyl or an alkoxyalkyl ester of the compound of the formula (I).
9. A process as claimed in claim 8 wherein the ester is the p-methoxybenzyl, p-bromobenzyl, p-nitrobenzyl, methyl or methoxylmethyl ester.
10. A process as claimed in claim 8 wherein the ester is the p-nitrobenzyl ester.
GB7930152A 1978-09-30 1979-08-31 Preparation of -lactam compounds Expired GB2035298B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB7930152A GB2035298B (en) 1978-09-30 1979-08-31 Preparation of -lactam compounds

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB7838837 1978-09-30
GB7930152A GB2035298B (en) 1978-09-30 1979-08-31 Preparation of -lactam compounds

Publications (2)

Publication Number Publication Date
GB2035298A true GB2035298A (en) 1980-06-18
GB2035298B GB2035298B (en) 1983-01-19

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Family Applications (1)

Application Number Title Priority Date Filing Date
GB7930152A Expired GB2035298B (en) 1978-09-30 1979-08-31 Preparation of -lactam compounds

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GB2035298B (en) 1983-01-19

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