GB2020014A - Bile acid assay - Google Patents
Bile acid assayInfo
- Publication number
- GB2020014A GB2020014A GB7911887A GB7911887A GB2020014A GB 2020014 A GB2020014 A GB 2020014A GB 7911887 A GB7911887 A GB 7911887A GB 7911887 A GB7911887 A GB 7911887A GB 2020014 A GB2020014 A GB 2020014A
- Authority
- GB
- United Kingdom
- Prior art keywords
- acid
- bile
- bile acid
- antiserum
- labelled
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003613 bile acid Substances 0.000 title abstract 7
- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical compound C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 title abstract 5
- 238000003556 assay Methods 0.000 title abstract 2
- 210000002966 serum Anatomy 0.000 abstract 3
- BHQCQFFYRZLCQQ-UHFFFAOYSA-N (3alpha,5alpha,7alpha,12alpha)-3,7,12-trihydroxy-cholan-24-oic acid Natural products OC1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 BHQCQFFYRZLCQQ-UHFFFAOYSA-N 0.000 abstract 1
- FWEOQOXTVHGIFQ-UHFFFAOYSA-N 8-anilinonaphthalene-1-sulfonic acid Chemical compound C=12C(S(=O)(=O)O)=CC=CC2=CC=CC=1NC1=CC=CC=C1 FWEOQOXTVHGIFQ-UHFFFAOYSA-N 0.000 abstract 1
- 102000014914 Carrier Proteins Human genes 0.000 abstract 1
- 239000004380 Cholic acid Substances 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 108091008324 binding proteins Proteins 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 abstract 1
- 229960002471 cholic acid Drugs 0.000 abstract 1
- 235000019416 cholic acid Nutrition 0.000 abstract 1
- KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- AXDXVEYHEODSPN-HVATVPOCSA-N lithocholic acid sulfate Chemical compound C([C@H]1CC2)[C@H](OS(O)(=O)=O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)CC1 AXDXVEYHEODSPN-HVATVPOCSA-N 0.000 abstract 1
- 230000014759 maintenance of location Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000000376 reactant Substances 0.000 abstract 1
- YGSDEFSMJLZEOE-UHFFFAOYSA-M salicylate Chemical compound OC1=CC=CC=C1C([O-])=O YGSDEFSMJLZEOE-UHFFFAOYSA-M 0.000 abstract 1
- 229960001860 salicylate Drugs 0.000 abstract 1
- RGHFKWPGWBFQLN-UHFFFAOYSA-M sodium;5,5-diethylpyrimidin-3-ide-2,4,6-trione Chemical compound [Na+].CCC1(CC)C([O-])=NC(=O)NC1=O RGHFKWPGWBFQLN-UHFFFAOYSA-M 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Endocrinology (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Bile acids, e.g. cholic acid, sulfolithocholic acid, or conjugates thereof in unextracted serum samples are assayed by: (a) providing an antiserum specific to the acid being determined, (b) mixing the unextracted serum sample with the antiserum and the same bile acid tagged with a labelling agent, (c) incubating the mixture from (b) to allow the bile acid and labelled bile acid to bind with antibodies in the antiserum, (d) separating the antibody-bound reactants from the unbound, (e) measuring the labelled bound fraction from (d) to find the quantity of bile acid in the sample by comparison with standards, wherein the assay is carried out in the presence of a binding inhibitor, e.g. 8-anilino-1-naphthalene-sulfonic acid, sodium barbital or salicylate, to inhibit retention of bile acids by binding proteins present in the serum sample.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US89991878A | 1978-04-26 | 1978-04-26 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB2020014A true GB2020014A (en) | 1979-11-07 |
| GB2020014B GB2020014B (en) | 1982-10-20 |
Family
ID=25411734
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB7911887A Expired GB2020014B (en) | 1978-04-26 | 1979-04-05 | Bile acid assay |
Country Status (11)
| Country | Link |
|---|---|
| JP (1) | JPS54149700A (en) |
| AU (1) | AU527381B2 (en) |
| BE (1) | BE875854A (en) |
| CA (1) | CA1093962A (en) |
| DE (1) | DE2916783C3 (en) |
| ES (1) | ES479985A1 (en) |
| FR (1) | FR2424536A1 (en) |
| GB (1) | GB2020014B (en) |
| IT (1) | IT1112580B (en) |
| NL (1) | NL7902396A (en) |
| SE (1) | SE7903645L (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0714028A1 (en) * | 1994-11-24 | 1996-05-29 | Yuugengaisha B.S.R. | Method for the measurement of serum bile acids by ELISA and method for the diagnosis of liver disease |
| CN106501535A (en) * | 2016-11-30 | 2017-03-15 | 长春迪瑞医疗科技股份有限公司 | A kind of compound releasing agent of ANS salt and the kit for detecting Blood cortisol |
| CN108700572A (en) * | 2016-02-29 | 2018-10-23 | 富士胶片株式会社 | Kit for quantifying bile acid in biological sample and method for quantifying bile acid in biological sample |
| CN109444399A (en) * | 2018-10-26 | 2019-03-08 | 重庆中元汇吉生物技术有限公司 | A kind of glycocholic acid detection kit |
| CN113721012A (en) * | 2021-08-27 | 2021-11-30 | 深圳上泰生物工程有限公司 | Composition and application of kit thereof in detection of glycocholic acid |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5474907A (en) * | 1994-03-25 | 1995-12-12 | Eastman Kodak Company | Multilayer analytical element for salicylate assay |
| WO2017150517A1 (en) | 2016-02-29 | 2017-09-08 | 富士フイルム株式会社 | Kit for determining quantity of substance to be assayed in biological sample |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4111656A (en) * | 1975-06-26 | 1978-09-05 | Mallinckrodt, Inc. | Radioimmunoassay methods for the determination of l-triiodothyronine and thyroxine |
| JPS5851000B2 (en) * | 1976-04-16 | 1983-11-14 | アボツト ラボラトリ−ズ | Production method of sulfolitocholylglycyltyramine |
-
1979
- 1979-03-27 NL NL7902396A patent/NL7902396A/en not_active Application Discontinuation
- 1979-03-30 CA CA324,498A patent/CA1093962A/en not_active Expired
- 1979-03-30 AU AU45634/79A patent/AU527381B2/en not_active Ceased
- 1979-04-05 GB GB7911887A patent/GB2020014B/en not_active Expired
- 1979-04-24 IT IT22140/79A patent/IT1112580B/en active
- 1979-04-24 FR FR7910391A patent/FR2424536A1/en active Pending
- 1979-04-24 JP JP4984979A patent/JPS54149700A/en active Pending
- 1979-04-25 DE DE2916783A patent/DE2916783C3/en not_active Expired
- 1979-04-25 BE BE0/194838A patent/BE875854A/en not_active IP Right Cessation
- 1979-04-25 SE SE7903645A patent/SE7903645L/en not_active Application Discontinuation
- 1979-04-26 ES ES479985A patent/ES479985A1/en not_active Expired
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0714028A1 (en) * | 1994-11-24 | 1996-05-29 | Yuugengaisha B.S.R. | Method for the measurement of serum bile acids by ELISA and method for the diagnosis of liver disease |
| US5631138A (en) * | 1994-11-24 | 1997-05-20 | Yuugengaisha B.S.R. | Method for the measurement of serum bile acids by elisa and method for the diagnosis of liver disease |
| CN108700572A (en) * | 2016-02-29 | 2018-10-23 | 富士胶片株式会社 | Kit for quantifying bile acid in biological sample and method for quantifying bile acid in biological sample |
| US10883986B2 (en) | 2016-02-29 | 2021-01-05 | Fujifilm Corporation | Kit for quantitatively determining bile acid in biological sample, and method for quantitatively determining bile acid in biological sample |
| CN106501535A (en) * | 2016-11-30 | 2017-03-15 | 长春迪瑞医疗科技股份有限公司 | A kind of compound releasing agent of ANS salt and the kit for detecting Blood cortisol |
| CN109444399A (en) * | 2018-10-26 | 2019-03-08 | 重庆中元汇吉生物技术有限公司 | A kind of glycocholic acid detection kit |
| CN113721012A (en) * | 2021-08-27 | 2021-11-30 | 深圳上泰生物工程有限公司 | Composition and application of kit thereof in detection of glycocholic acid |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS54149700A (en) | 1979-11-24 |
| AU527381B2 (en) | 1983-03-03 |
| DE2916783A1 (en) | 1979-10-31 |
| DE2916783B2 (en) | 1981-07-16 |
| NL7902396A (en) | 1979-10-30 |
| IT7922140A0 (en) | 1979-04-24 |
| DE2916783C3 (en) | 1982-04-01 |
| BE875854A (en) | 1979-10-25 |
| FR2424536A1 (en) | 1979-11-23 |
| IT1112580B (en) | 1986-01-20 |
| ES479985A1 (en) | 1980-08-16 |
| SE7903645L (en) | 1979-10-27 |
| GB2020014B (en) | 1982-10-20 |
| CA1093962A (en) | 1981-01-20 |
| AU4563479A (en) | 1979-11-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PCNP | Patent ceased through non-payment of renewal fee |