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GB201314565D0 - Method and apparatus for improved resolution chromatography - Google Patents

Method and apparatus for improved resolution chromatography

Info

Publication number
GB201314565D0
GB201314565D0 GBGB1314565.1A GB201314565A GB201314565D0 GB 201314565 D0 GB201314565 D0 GB 201314565D0 GB 201314565 A GB201314565 A GB 201314565A GB 201314565 D0 GB201314565 D0 GB 201314565D0
Authority
GB
United Kingdom
Prior art keywords
column
flow
portions
inlet
eluate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GBGB1314565.1A
Other versions
GB2504622A (en
GB2504622B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Western Sydney
Thermo Electron Manufacturing Ltd
Original Assignee
University of Western Sydney
Thermo Electron Manufacturing Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Western Sydney, Thermo Electron Manufacturing Ltd filed Critical University of Western Sydney
Priority to GB1314565.1A priority Critical patent/GB2504622B/en
Publication of GB201314565D0 publication Critical patent/GB201314565D0/en
Publication of GB2504622A publication Critical patent/GB2504622A/en
Application granted granted Critical
Publication of GB2504622B publication Critical patent/GB2504622B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/14—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/24—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the treatment of the fractions to be distributed
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G01N30/6017—Fluid distributors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G01N30/603—Construction of the column end pieces retaining the stationary phase, e.g. Frits
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G01N30/6086—Construction of the column body form designed to optimise dispersion
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/80—Fraction collectors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

An apparatus and a method are provided for column chromatography, comprising a cylindrical chromatography column 15, the column having an inlet and an outlet 411. The inlet is configured to introduce a flow of mobile phase 2 into the column carrying a sample, whereby the sample separates into components as it advances longitudinally through the column from the inlet to the outlet carried by the mobile phase, wherein the inlet is further configured to introduce the flow of mobile phase into the column in at least two separate portions which are independently controllable, and to introduce the portions into different radial regions 440, 442 of the column, such that the portions flow longitudinally through the column in different radial regions, and wherein the mobile phase leaves the column as a flow of eluate and the outlet is configured to split the flow of eluate as it leaves the column into at least two separate eluate portions from the different radial regions of the column and the apparatus is configured to separately process the eluate portions.
GB1314565.1A 2011-05-26 2011-05-26 Method and apparatus for improved resolution chromatography Active GB2504622B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1314565.1A GB2504622B (en) 2011-05-26 2011-05-26 Method and apparatus for improved resolution chromatography

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1314565.1A GB2504622B (en) 2011-05-26 2011-05-26 Method and apparatus for improved resolution chromatography
GB1108859.8A GB2491168B (en) 2011-05-26 2011-05-26 Method and apparatus for improved resolution chromatography

Publications (3)

Publication Number Publication Date
GB201314565D0 true GB201314565D0 (en) 2013-09-25
GB2504622A GB2504622A (en) 2014-02-05
GB2504622B GB2504622B (en) 2015-09-16

Family

ID=44279655

Family Applications (2)

Application Number Title Priority Date Filing Date
GB1108859.8A Active GB2491168B (en) 2011-05-26 2011-05-26 Method and apparatus for improved resolution chromatography
GB1314565.1A Active GB2504622B (en) 2011-05-26 2011-05-26 Method and apparatus for improved resolution chromatography

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB1108859.8A Active GB2491168B (en) 2011-05-26 2011-05-26 Method and apparatus for improved resolution chromatography

Country Status (6)

Country Link
JP (1) JP6002212B2 (en)
CN (1) CN103687654B (en)
AU (1) AU2012260981B2 (en)
DE (1) DE112012002247B4 (en)
GB (2) GB2491168B (en)
WO (1) WO2012160032A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170110716A (en) * 2015-02-09 2017-10-11 막스-플랑크-게젤샤프트 츄어 푀르더룽 데어 비쎈샤프텐 에.파우. Means and methods for minimizing sweep and dead volume in chromatographic applications
CN104849378B (en) * 2015-04-29 2017-03-15 华东理工大学 The capillary column system for increasing open tubular column applied sample amount using reducing packed column
CN110291392B (en) * 2017-02-13 2021-08-20 株式会社岛津制作所 supercritical fluid device
CN109406689B (en) * 2018-10-22 2023-05-12 南京国科精准医学科技有限公司 Ion permeation gas molecular separation method and device
CA3182368A1 (en) 2020-05-01 2021-11-04 Kashiv Biosciences, Llc An improved process of purification of protein
CA3255003A1 (en) * 2022-03-23 2023-09-28 Kashiv Biosciences, Llc An improved process for the separation and analysis of pancreatic enzymes
US12493018B2 (en) 2022-06-27 2025-12-09 Agilent Technologies, Inc. Load adapter for improved packing bed stability
CN119972207B (en) * 2025-01-22 2025-12-26 北京工业大学 Two-dimensional liquid chromatography microfluidic chip and its fabrication method

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UST959004I4 (en) * 1976-05-26 1977-06-07 E. I. Du Pont De Nemours And Company Chromatographic liquid dispensing inlet and method
JPS54139596A (en) * 1978-04-20 1979-10-30 Shimadzu Corp Liquid chromatography
US4537217A (en) 1982-12-09 1985-08-27 Research Triangle Institute Fluid distributor
JPH037815Y2 (en) * 1984-10-05 1991-02-26
JPS6253857A (en) * 1985-08-31 1987-03-09 Fujitsu Ltd Printing density control system of printer
JPS6263857A (en) * 1985-09-13 1987-03-20 Japan Spectroscopic Co Method and instrument for separating and analyzing component in fluid
US5124133A (en) * 1985-10-31 1992-06-23 Wilmer Schoenrock Apparatus and method for providing a uniform flow profile through large diameter, low-pressure vessels
JPS62240857A (en) * 1986-04-14 1987-10-21 Kurita Water Ind Ltd Chromatography apparatus
CS259190B1 (en) 1986-08-21 1988-10-14 Bedrich Porsch Column for preparative liquid chromatography
US4915843A (en) * 1988-11-28 1990-04-10 Union Oil Company Of California Continuous displacement chromatographic method
JPH0776767B2 (en) 1988-12-10 1995-08-16 株式会社日立製作所 Column for liquid chromatography
US4999102A (en) 1988-12-16 1991-03-12 The Amalgamated Sugar Company Liquid transfer manifold system for maintaining plug flow
JP3975575B2 (en) * 1998-09-11 2007-09-12 株式会社島津製作所 column
CN101206205B (en) * 2006-12-22 2011-08-10 中国科学院大连化学物理研究所 Method and isolated device for micro flux liquid chromatogram on-line large volume sample injection

Also Published As

Publication number Publication date
CN103687654A (en) 2014-03-26
AU2012260981B2 (en) 2015-09-10
DE112012002247T5 (en) 2014-04-10
GB2504622A (en) 2014-02-05
JP6002212B2 (en) 2016-10-05
GB2491168A (en) 2012-11-28
WO2012160032A1 (en) 2012-11-29
CN103687654B (en) 2016-03-16
JP2014517924A (en) 2014-07-24
GB201108859D0 (en) 2011-07-06
GB2491168B (en) 2014-09-10
GB2504622B (en) 2015-09-16
DE112012002247B4 (en) 2022-05-05

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