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GB201209896D0 - Process - Google Patents

Process

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Publication number
GB201209896D0
GB201209896D0 GB201209896A GB201209896A GB201209896D0 GB 201209896 D0 GB201209896 D0 GB 201209896D0 GB 201209896 A GB201209896 A GB 201209896A GB 201209896 A GB201209896 A GB 201209896A GB 201209896 D0 GB201209896 D0 GB 201209896D0
Authority
GB
United Kingdom
Prior art keywords
crm197
vector
operably linked
gene encoding
coli host
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
GB201209896A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Diosynth Biotechnologies UK Ltd
Original Assignee
Fujifilm Diosynth Biotechnologies UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujifilm Diosynth Biotechnologies UK Ltd filed Critical Fujifilm Diosynth Biotechnologies UK Ltd
Priority to GB201209896A priority Critical patent/GB201209896D0/en
Publication of GB201209896D0 publication Critical patent/GB201209896D0/en
Priority to PCT/GB2013/000238 priority patent/WO2013178974A1/en
Ceased legal-status Critical Current

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

In one aspect, a process for the intracellular expression of CRM197 in an E coli host is provided. The process comprises expressing a vector comprising a gene encoding CRM 197 operably linked to a promoter and at least one perfect palindrome operator sequence. In a related aspect, a process for the periplasmic expression of CRM197 in an E coli host is provided, comprising expressing a vector comprising a gene encoding CRM197 and a secretion leader sequence operably linked to a weak promoter system comprising at least one perfect palindrome operator sequence.
GB201209896A 2012-06-01 2012-06-01 Process Ceased GB201209896D0 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB201209896A GB201209896D0 (en) 2012-06-01 2012-06-01 Process
PCT/GB2013/000238 WO2013178974A1 (en) 2012-06-01 2013-05-23 Process for expression of crm197

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB201209896A GB201209896D0 (en) 2012-06-01 2012-06-01 Process

Publications (1)

Publication Number Publication Date
GB201209896D0 true GB201209896D0 (en) 2012-07-18

Family

ID=46582288

Family Applications (1)

Application Number Title Priority Date Filing Date
GB201209896A Ceased GB201209896D0 (en) 2012-06-01 2012-06-01 Process

Country Status (2)

Country Link
GB (1) GB201209896D0 (en)
WO (1) WO2013178974A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102282930B1 (en) * 2014-01-31 2021-07-27 피나 바이오솔루션스, 엘엘씨 Expression and purification of crm197 and related proteins
US11060123B2 (en) 2014-01-31 2021-07-13 Fina Biosolutions, Llc Production of soluble recombinant protein without n-terminal methionine
US12104161B2 (en) 2014-01-31 2024-10-01 Fina Biosolutions Llc Production of soluble recombinant proteins without N-terminal methionine in E-coli
WO2016079755A1 (en) 2014-11-20 2016-05-26 Biological E Limited Codon optimized polynucleotide for high level expression of crm197
GB201704659D0 (en) 2017-03-24 2017-05-10 Fujifilm Diosynth Biotechnologies Uk Ltd Expression system
KR102099342B1 (en) 2019-09-03 2020-04-10 주식회사 제노포커스 Expression Method of CRM197 Protein

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9715660D0 (en) * 1997-07-25 1997-10-01 Zeneca Ltd Proteins
GB9904582D0 (en) 1999-02-26 1999-04-21 Nycomed Imaging As Process
GB0602173D0 (en) 2006-02-03 2006-03-15 Avecia Ltd Expression system
AU2010201410B2 (en) 2010-03-30 2015-04-30 Pelican Technology Holdings, Inc. High level expression of recombinant CRM197

Also Published As

Publication number Publication date
WO2013178974A1 (en) 2013-12-05

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Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)