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GB201111637D0 - A process for producing amides and acids using a nitrile-metabolising enzyme - Google Patents

A process for producing amides and acids using a nitrile-metabolising enzyme

Info

Publication number
GB201111637D0
GB201111637D0 GBGB1111637.3A GB201111637A GB201111637D0 GB 201111637 D0 GB201111637 D0 GB 201111637D0 GB 201111637 A GB201111637 A GB 201111637A GB 201111637 D0 GB201111637 D0 GB 201111637D0
Authority
GB
United Kingdom
Prior art keywords
nitrile
acids
polypeptide
metabolising enzyme
metabolising
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
GBGB1111637.3A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Waterford Institute of Technology
Original Assignee
Waterford Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Waterford Institute of Technology filed Critical Waterford Institute of Technology
Priority to GBGB1111637.3A priority Critical patent/GB201111637D0/en
Publication of GB201111637D0 publication Critical patent/GB201111637D0/en
Priority to PCT/EP2012/063315 priority patent/WO2013004832A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/002—Nitriles (-CN)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to an isolated nucleotide sequence andcorresponding polypeptide derived from the nitrile-metabolising Pantoea strain deposited under NCIMB 41853. Saidisolated polypeptide actsas a nitrilase and the invention extends to a process for producing a carboxylic acid using said isolated polypeptide to metabolise nitriles such as 3-hydroxyglutaronitrile, 3-hydroxybutyronitrile and 3- hydroxy-phenylpropionitrile to form corresponding carboxylic acids.
GBGB1111637.3A 2011-07-07 2011-07-07 A process for producing amides and acids using a nitrile-metabolising enzyme Ceased GB201111637D0 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GBGB1111637.3A GB201111637D0 (en) 2011-07-07 2011-07-07 A process for producing amides and acids using a nitrile-metabolising enzyme
PCT/EP2012/063315 WO2013004832A1 (en) 2011-07-07 2012-07-06 A nitrile-metabolising enzyme and a process for producing an acid using same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB1111637.3A GB201111637D0 (en) 2011-07-07 2011-07-07 A process for producing amides and acids using a nitrile-metabolising enzyme

Publications (1)

Publication Number Publication Date
GB201111637D0 true GB201111637D0 (en) 2011-08-24

Family

ID=44544373

Family Applications (1)

Application Number Title Priority Date Filing Date
GBGB1111637.3A Ceased GB201111637D0 (en) 2011-07-07 2011-07-07 A process for producing amides and acids using a nitrile-metabolising enzyme

Country Status (2)

Country Link
GB (1) GB201111637D0 (en)
WO (1) WO2013004832A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119823972B (en) * 2023-10-13 2025-10-31 中国科学院天津工业生物技术研究所 Nitrile hydrolases and their application in the preparation of p-cyanobenzoic acid

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4405258B2 (en) * 2001-06-21 2010-01-27 ヴェレニウム コーポレイション Nitrilase
US9217164B2 (en) * 2007-03-01 2015-12-22 Basf Enzymes Llc Nitrilases, nucleic acids encoding them and methods for making and using them

Also Published As

Publication number Publication date
WO2013004832A1 (en) 2013-01-10

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Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)