[go: up one dir, main page]

GB201108259D0 - Gel beads in microfluidic droplets - Google Patents

Gel beads in microfluidic droplets

Info

Publication number
GB201108259D0
GB201108259D0 GBGB1108259.1A GB201108259A GB201108259D0 GB 201108259 D0 GB201108259 D0 GB 201108259D0 GB 201108259 A GB201108259 A GB 201108259A GB 201108259 D0 GB201108259 D0 GB 201108259D0
Authority
GB
United Kingdom
Prior art keywords
gel beads
microfluidic droplets
microfluidic
droplets
beads
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
GBGB1108259.1A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cambridge Enterprise Ltd
Original Assignee
Cambridge Enterprise Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge Enterprise Ltd filed Critical Cambridge Enterprise Ltd
Priority to GBGB1108259.1A priority Critical patent/GB201108259D0/en
Publication of GB201108259D0 publication Critical patent/GB201108259D0/en
Priority to PCT/GB2012/051106 priority patent/WO2012156744A2/en
Ceased legal-status Critical Current

Links

GBGB1108259.1A 2011-05-17 2011-05-17 Gel beads in microfluidic droplets Ceased GB201108259D0 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GBGB1108259.1A GB201108259D0 (en) 2011-05-17 2011-05-17 Gel beads in microfluidic droplets
PCT/GB2012/051106 WO2012156744A2 (en) 2011-05-17 2012-05-17 Gel beads in microfluidic droplets

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB1108259.1A GB201108259D0 (en) 2011-05-17 2011-05-17 Gel beads in microfluidic droplets

Publications (1)

Publication Number Publication Date
GB201108259D0 true GB201108259D0 (en) 2011-06-29

Family

ID=44260665

Family Applications (1)

Application Number Title Priority Date Filing Date
GBGB1108259.1A Ceased GB201108259D0 (en) 2011-05-17 2011-05-17 Gel beads in microfluidic droplets

Country Status (2)

Country Link
GB (1) GB201108259D0 (en)
WO (1) WO2012156744A2 (en)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
CN104364392B (en) 2012-02-27 2018-05-25 赛卢拉研究公司 For the composition and kit of numerator counts
WO2014093976A1 (en) * 2012-12-14 2014-06-19 Gnubio, Inc. Method for maintaining heterogeneous concentrations of molecules in emulsion droplets
KR102758333B1 (en) 2013-08-28 2025-01-23 벡톤 디킨슨 앤드 컴퍼니 Massively parallel single cell analysis
US20160279068A1 (en) 2013-11-08 2016-09-29 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
CN116064731A (en) 2015-03-13 2023-05-05 哈佛学院院长及董事 Determining Cells Using Expansion
CN107406889B (en) 2015-03-20 2021-11-02 诺维信公司 Droplet-based selection by injection
EP3277843A2 (en) 2015-03-30 2018-02-07 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
JP6940484B2 (en) 2015-09-11 2021-09-29 セルラー リサーチ, インコーポレイテッド Methods and compositions for library normalization
US11123297B2 (en) 2015-10-13 2021-09-21 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
US10233487B2 (en) * 2016-03-24 2019-03-19 Bio-Rad Laboratories, Inc. Use of gel beads to control droplet dispersion
WO2017184707A1 (en) * 2016-04-19 2017-10-26 President And Fellows Of Harvard College Immobilization-based systems and methods for genetic analysis and other applications
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
EP4300099A3 (en) 2016-09-26 2024-03-27 Becton, Dickinson and Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
WO2018067789A1 (en) * 2016-10-07 2018-04-12 President And Fellows Of Harvard College Hydrogel display
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
GB201702938D0 (en) * 2017-02-23 2017-04-12 Univ Southampton Methods of generating and screening compartmentalised peptides libraries
US12054764B2 (en) 2017-04-14 2024-08-06 The Broad Institute, Inc. High-throughput screens for exploring biological functions of microscale biological systems
CN117143960A (en) * 2017-05-18 2023-12-01 10X基因组学有限公司 Methods and systems for sorting droplets and beads
US10544413B2 (en) * 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP4345172A3 (en) 2017-06-05 2024-07-03 Becton, Dickinson and Company Sample indexing for single cells
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
US10501739B2 (en) * 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Microfluidic channel networks for partitioning
CN111206081B (en) * 2018-11-21 2023-06-30 思纳福(苏州)生命科技有限公司 Nucleic acid detection microsphere, preparation method, kit and high-throughput nucleic acid detection method
WO2019169347A1 (en) 2018-03-02 2019-09-06 10X Genomics, Inc. Systems and apparatus for holding plates
JP7407128B2 (en) 2018-05-03 2023-12-28 ベクトン・ディキンソン・アンド・カンパニー High-throughput multi-omics sample analysis
WO2019213237A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
US11639517B2 (en) 2018-10-01 2023-05-02 Becton, Dickinson And Company Determining 5′ transcript sequences
US20210382061A1 (en) * 2018-10-22 2021-12-09 The General Hospital Corporation Multiplexed single-cell analysis using optically-encoded rna capture particles
EP3877520B1 (en) 2018-11-08 2025-03-19 Becton, Dickson And Company Whole transcriptome analysis of single cells using random priming
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
JP2022514594A (en) * 2018-12-20 2022-02-14 スキルセル Biodegradable biochemical sensors for determining the presence and / or levels of pesticides or endocrine disruptors: methods and compositions.
US11661631B2 (en) 2019-01-23 2023-05-30 Becton, Dickinson And Company Oligonucleotides associated with antibodies
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
EP3930900A1 (en) 2019-02-28 2022-01-05 10X Genomics, Inc. Devices, systems, and methods for increasing droplet formation efficiency
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
CA3138806A1 (en) 2019-05-22 2020-11-26 Dalia Dhingra Method and apparatus for simultaneous targeted sequencing of dna, rna and protein
WO2021003255A1 (en) 2019-07-01 2021-01-07 Mission Bio Method and apparatus to normalize quantitative readouts in single-cell experiments
FR3098128B1 (en) * 2019-07-05 2023-11-17 Commissariat Energie Atomique Microfluidic device comprising a microdrop having a sol-gel matrix.
CN120099137A (en) 2019-07-22 2025-06-06 贝克顿迪金森公司 Single-cell chromatin immunoprecipitation sequencing assay
CN114729350A (en) 2019-11-08 2022-07-08 贝克顿迪金森公司 Obtaining full-length V (D) J information for immunohistorian sequencing using random priming
CN114902024A (en) * 2019-11-15 2022-08-12 闪璨迈璀公司 Compositions and methods based on fluorophore diffusion
US11857981B2 (en) 2019-12-23 2024-01-02 10X Genomics, Inc. Magnetic separator for an automated single cell sequencing system
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
CN115335520A (en) 2020-01-29 2022-11-11 贝克顿迪金森公司 Barcoded wells for spatial mapping of single cells by sequencing
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
ES2987035T3 (en) 2020-06-02 2024-11-13 Becton Dickinson Co Oligonucleotides and beads for gene expression assay 5
US12263482B1 (en) 2020-06-03 2025-04-01 10X Genomics, Inc. Methods and devices for magnetic separation in a flow path
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CN114058088A (en) * 2020-07-29 2022-02-18 京东方科技集团股份有限公司 Gel precursor, nucleic acid amplification reagent gel, memory chip and using method
CN116194589A (en) 2020-07-31 2023-05-30 贝克顿迪金森公司 Single-cell assays for transposase-accessible chromatin
CN112295616B (en) * 2020-09-11 2022-03-18 东南大学 Micro-droplet mixing reinforced micro-fluidic device
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
WO2022132206A1 (en) 2020-12-15 2022-06-23 Becton, Dickinson And Company Single cell secretome analysis
CN114184452B (en) * 2021-11-30 2024-03-01 阿里生物技术泰州有限公司 Dye bead for centrifugal microfluidic disc chip and preparation method thereof
EP4617661A1 (en) * 2024-03-14 2025-09-17 ETH Zurich Cell-free expression and screening microfluidic platform for peptide drug discovery

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2792122C (en) * 1997-07-07 2015-09-08 Medical Research Council In vitro sorting method
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
GB0221053D0 (en) * 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
WO2005049787A2 (en) * 2003-11-24 2005-06-02 Yeda Research And Development Co.Ltd. Compositions and methods for in vitro sorting of molecular and cellular libraries
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
CN101413034B (en) * 2008-11-21 2011-02-09 东南大学 Method for preparing molecular cloning chip by high-throughput nucleic acid molecular cloning
CN101736087A (en) * 2010-01-28 2010-06-16 华东医学生物技术研究所 Method for digitally detecting micro-mutation by using micro-emulsion clone amplified bound water gel microsphere chip

Also Published As

Publication number Publication date
WO2012156744A3 (en) 2013-01-10
WO2012156744A2 (en) 2012-11-22

Similar Documents

Publication Publication Date Title
GB201108259D0 (en) Gel beads in microfluidic droplets
IL281148A (en) Novel kif5b-ret fusion molecules and uses thereof
IL231983A0 (en) Tricyclo-phosphorothioate dna
GB201414967D0 (en) Microfluidic cartridge
AP2012000425S (en) Automobile
GB201211342D0 (en) Microfluidic device
SG11201400130RA (en) Microfluidic device
GB2509440B (en) Microfluidic device with interconnects
GB2514719B (en) Vehicular handle
IL236153A0 (en) Structurall member in framework structures
EP2707756A4 (en) True-amplitude layer-stripping in fractured media
SG11201405814WA (en) Improvements in fluid conditioning
GB2504205B (en) Improvements in vehicles
PT2755765T (en) Microfluidic device
GB201108951D0 (en) Air vehicles
EP2867155A4 (en) Nanostructure with functionally different sides
GB2500986B (en) Reagent bead inserter
GB2497781B (en) Liquid pourer
PL396187A1 (en) Vehicle simulator
HUP1200389A2 (en) Microfluidic disc
HUP1200504A2 (en) Driving simulator
AU2012901312A0 (en) Improvements In Fluid Conditioning
ZA201108197B (en) Gel
SG2014011332A (en) Microfluidic device and microfluidic system
GB201200113D0 (en) Improvements in agglomerating materials

Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)