GB2011070A - Assay of vitamin B12 - Google Patents
Assay of vitamin B12Info
- Publication number
- GB2011070A GB2011070A GB7847509A GB7847509A GB2011070A GB 2011070 A GB2011070 A GB 2011070A GB 7847509 A GB7847509 A GB 7847509A GB 7847509 A GB7847509 A GB 7847509A GB 2011070 A GB2011070 A GB 2011070A
- Authority
- GB
- United Kingdom
- Prior art keywords
- vitamin
- assay
- solution
- chicken serum
- proteins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229930003779 Vitamin B12 Natural products 0.000 title abstract 3
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 title abstract 3
- 239000011715 vitamin B12 Substances 0.000 title abstract 3
- 235000019163 vitamin B12 Nutrition 0.000 title abstract 3
- 238000003556 assay Methods 0.000 title abstract 2
- 241000287828 Gallus gallus Species 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 102000004506 Blood Proteins Human genes 0.000 abstract 1
- 108010017384 Blood Proteins Proteins 0.000 abstract 1
- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 abstract 1
- 102000011409 Transcobalamins Human genes 0.000 abstract 1
- 108010023603 Transcobalamins Proteins 0.000 abstract 1
- 230000009137 competitive binding Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/82—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving vitamins or their receptors
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The amount of vitamin B12 in solution in a liquid is determined by mixing a sample of the solution with vitamin B12-binding proteins from chicken serum in a pH of about 12.8 to 13.2, and then analysing a component of the mixture. In preferred procedures, especially useful in assaying human sera, a competitive binding technique is employed utilizing labelled vitamin B12 and a buffer containing cyanide ion. By operating at very high pH and with chicken serum proteins, the assay can be conducted relatively simply and with very accurate results.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB7847509A GB2011070B (en) | 1977-12-14 | 1978-12-07 | Assay of vitamin b12 |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB5206977 | 1977-12-14 | ||
| GB7847509A GB2011070B (en) | 1977-12-14 | 1978-12-07 | Assay of vitamin b12 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB2011070A true GB2011070A (en) | 1979-07-04 |
| GB2011070B GB2011070B (en) | 1982-07-14 |
Family
ID=26266987
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB7847509A Expired GB2011070B (en) | 1977-12-14 | 1978-12-07 | Assay of vitamin b12 |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB2011070B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3137668A1 (en) | 1980-09-22 | 1982-05-27 | Amersham International Ltd., Amersham, Buckinghamshire | METHOD FOR TESTING FOR VITAMIN B (DOWN ARROW) 1 (DOWN ARROW) (DOWN ARROW) 2 (DOWN ARROW) AND TEST SET FOR ITS IMPLEMENTATION |
| US4332786A (en) * | 1980-05-15 | 1982-06-01 | Baxter Travenol Laboratories, Inc. | Method and composition for vitamin B-12 Assay |
| USD1107249S1 (en) * | 2022-11-28 | 2025-12-23 | Schalcon S.P.A. | Contact lens disposal container |
-
1978
- 1978-12-07 GB GB7847509A patent/GB2011070B/en not_active Expired
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4332786A (en) * | 1980-05-15 | 1982-06-01 | Baxter Travenol Laboratories, Inc. | Method and composition for vitamin B-12 Assay |
| DE3137668A1 (en) | 1980-09-22 | 1982-05-27 | Amersham International Ltd., Amersham, Buckinghamshire | METHOD FOR TESTING FOR VITAMIN B (DOWN ARROW) 1 (DOWN ARROW) (DOWN ARROW) 2 (DOWN ARROW) AND TEST SET FOR ITS IMPLEMENTATION |
| USD1107249S1 (en) * | 2022-11-28 | 2025-12-23 | Schalcon S.P.A. | Contact lens disposal container |
Also Published As
| Publication number | Publication date |
|---|---|
| GB2011070B (en) | 1982-07-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 732 | Registration of transactions, instruments or events in the register (sect. 32/1977) | ||
| PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 19941207 |