GB1502674A - Microorganism culturing and testing apparatus - Google Patents
Microorganism culturing and testing apparatusInfo
- Publication number
- GB1502674A GB1502674A GB16645/75A GB1664575A GB1502674A GB 1502674 A GB1502674 A GB 1502674A GB 16645/75 A GB16645/75 A GB 16645/75A GB 1664575 A GB1664575 A GB 1664575A GB 1502674 A GB1502674 A GB 1502674A
- Authority
- GB
- United Kingdom
- Prior art keywords
- cellulose
- water
- pad
- composition
- nutrient
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000012258 culturing Methods 0.000 title abstract 2
- 244000005700 microbiome Species 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 abstract 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Substances OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 abstract 5
- 235000015097 nutrients Nutrition 0.000 abstract 4
- 239000000126 substance Substances 0.000 abstract 4
- -1 polyethylene Polymers 0.000 abstract 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract 2
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 abstract 2
- 239000004793 Polystyrene Substances 0.000 abstract 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 abstract 2
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric Acid Chemical compound [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 abstract 2
- 239000002253 acid Substances 0.000 abstract 2
- 239000002585 base Substances 0.000 abstract 2
- 229920002301 cellulose acetate Polymers 0.000 abstract 2
- 239000001863 hydroxypropyl cellulose Substances 0.000 abstract 2
- 235000010977 hydroxypropyl cellulose Nutrition 0.000 abstract 2
- 230000004048 modification Effects 0.000 abstract 2
- 238000012986 modification Methods 0.000 abstract 2
- 229920002223 polystyrene Polymers 0.000 abstract 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 abstract 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 abstract 1
- QISOBCMNUJQOJU-UHFFFAOYSA-N 4-bromo-1h-pyrazole-5-carboxylic acid Chemical compound OC(=O)C=1NN=CC=1Br QISOBCMNUJQOJU-UHFFFAOYSA-N 0.000 abstract 1
- PXRKCOCTEMYUEG-UHFFFAOYSA-N 5-aminoisoindole-1,3-dione Chemical compound NC1=CC=C2C(=O)NC(=O)C2=C1 PXRKCOCTEMYUEG-UHFFFAOYSA-N 0.000 abstract 1
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 abstract 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 abstract 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 abstract 1
- 241000206575 Chondrus crispus Species 0.000 abstract 1
- 239000001856 Ethyl cellulose Substances 0.000 abstract 1
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 abstract 1
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Natural products OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 abstract 1
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 abstract 1
- 239000004354 Hydroxyethyl cellulose Substances 0.000 abstract 1
- 241000588652 Neisseria gonorrhoeae Species 0.000 abstract 1
- 239000000020 Nitrocellulose Substances 0.000 abstract 1
- 239000005062 Polybutadiene Substances 0.000 abstract 1
- 239000004698 Polyethylene Substances 0.000 abstract 1
- 239000004372 Polyvinyl alcohol Substances 0.000 abstract 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 abstract 1
- RAHZWNYVWXNFOC-UHFFFAOYSA-N Sulphur dioxide Chemical class O=S=O RAHZWNYVWXNFOC-UHFFFAOYSA-N 0.000 abstract 1
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 abstract 1
- 150000007513 acids Chemical class 0.000 abstract 1
- 229940072056 alginate Drugs 0.000 abstract 1
- 235000010443 alginic acid Nutrition 0.000 abstract 1
- 229920000615 alginic acid Polymers 0.000 abstract 1
- 229910052783 alkali metal Inorganic materials 0.000 abstract 1
- 229910000288 alkali metal carbonate Inorganic materials 0.000 abstract 1
- 150000008041 alkali metal carbonates Chemical class 0.000 abstract 1
- 229910052784 alkaline earth metal Inorganic materials 0.000 abstract 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 abstract 1
- 150000008064 anhydrides Chemical class 0.000 abstract 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 abstract 1
- 239000001569 carbon dioxide Substances 0.000 abstract 1
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract 1
- 150000004649 carbonic acid derivatives Chemical class 0.000 abstract 1
- 239000001768 carboxy methyl cellulose Substances 0.000 abstract 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 abstract 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 abstract 1
- 229920002678 cellulose Polymers 0.000 abstract 1
- 239000001913 cellulose Substances 0.000 abstract 1
- 229920006217 cellulose acetate butyrate Polymers 0.000 abstract 1
- 229920001249 ethyl cellulose Polymers 0.000 abstract 1
- 235000019325 ethyl cellulose Nutrition 0.000 abstract 1
- 239000001530 fumaric acid Substances 0.000 abstract 1
- 239000011521 glass Substances 0.000 abstract 1
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 abstract 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 abstract 1
- 239000011976 maleic acid Substances 0.000 abstract 1
- 239000001630 malic acid Substances 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 229920001220 nitrocellulos Polymers 0.000 abstract 1
- 150000007524 organic acids Chemical class 0.000 abstract 1
- 229920003023 plastic Polymers 0.000 abstract 1
- 239000004033 plastic Substances 0.000 abstract 1
- 229920002401 polyacrylamide Polymers 0.000 abstract 1
- 229920002857 polybutadiene Polymers 0.000 abstract 1
- 229920000573 polyethylene Polymers 0.000 abstract 1
- 229920002451 polyvinyl alcohol Polymers 0.000 abstract 1
- 239000000376 reactant Substances 0.000 abstract 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 abstract 1
- 235000017557 sodium bicarbonate Nutrition 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-L sulfite Chemical compound [O-]S([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-L 0.000 abstract 1
- 239000011975 tartaric acid Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- 229920003176 water-insoluble polymer Polymers 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/045—Culture media therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
1502674 Compositions for generating CO 2 or SO 2 MILES LABORATORIES Inc 22 April 1975 [25 April 1974(3)] 16645/75 Heading C 1A [Also in Divisions C6 and G1] Apparatus for culturing and identifying a microorganism in an anaerobic environment, e.g. Neisseria gonorrhoeae in an atomosphere of carbon dioxide, consists in a preferred embodiment of a polystyrene base 10, Fig. 1, having an upstanding rectangular wall 12 and a transverse wall 21 having an upper surface 22 below the level of the upper surface 19 of wall 12, and a polyethylene cover 17. The walls 12 and 21 define spaced rectangular wells 13 and 14 in which are disposed a nutrient gel layer or dry nutrient pad 15 and a gas-generating composition in the form of a pad or film 16 responsive to water vapour evolved by the reconstituted nutrient medium. In a modification the gasgenerating composition is carried by the cover. In other embodiments, a rectangular base has the nutrient pad and a gas generating pad or film attached to it, and the assembly is enclosed in a sealable plastics bag. The carbon-dioxide-generating composition includes, additional to the conventional acid and carbonate, a water-soluble polymeric substance and a water-insoluble polymeric substance in relative proportions between 1:9 and 9:1, preferably between 1:3 and 3:1, by weight. The water-insoluble polymer moderates the evolution of gas and also serves as a supporting matrix. Water-soluble polymeric substances: hydroxypropyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, polyacrylamide or polyvinyl alcohol. Water-insoluble polymeric substances: cellulose acetate, cellulose nitrate, cellulose acetate butyrate, ethyl cellulose, butyl cellulose, polybutadiene or polystyrene, also alginate or carrageen. Acids (conventional): citric, malic, fumaric, tartaric or maleic acid, or a corresponding anhydride. Carbonates (conventional): dibasic alkali metal carbonate, alkali metal bicarbonate or alkaline earth metal carbonate. Example 2 discloses the preparation of films or pads of effervescent composition in which acetone solutions of hydroxypropyl cellulose and cellulose acetate (ratios 3:1, 2:2, 1:3) containing citric acid and sodium bicarbonate were cast on glass plates or filter paper and the solvent allowed to evaporate. According to a modification, a steady, controlled humidity-activated sulphur dioxide source may be similarly provided by using an organic acid and an inorganic sulphite as the solid reactants in such a composition.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US46410874A | 1974-04-25 | 1974-04-25 | |
| US46403174A | 1974-04-25 | 1974-04-25 | |
| US464107A US3888741A (en) | 1974-04-25 | 1974-04-25 | Culturing means and test kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1502674A true GB1502674A (en) | 1978-03-01 |
Family
ID=27412916
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB16645/75A Expired GB1502674A (en) | 1974-04-25 | 1975-04-22 | Microorganism culturing and testing apparatus |
Country Status (4)
| Country | Link |
|---|---|
| JP (1) | JPS50145576A (en) |
| DE (1) | DE2518282A1 (en) |
| FR (1) | FR2268863B1 (en) |
| GB (1) | GB1502674A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0017414A1 (en) * | 1979-04-02 | 1980-10-15 | FMC Corporation | Device for delivering measured quantities of reagent to an assay medium and method of assaying a liquid aqueous assay medium |
| GB2157708A (en) * | 1984-01-24 | 1985-10-30 | Eric James Sjoberg | Collecting and preserving microorganisms |
| US5494823A (en) * | 1992-01-21 | 1996-02-27 | Shimakyu Chemical Co. Ltd. | Apparatus for culturing microorganism |
| CN110463832A (en) * | 2019-08-13 | 2019-11-19 | 广州悦蜂生物防治科技有限公司 | A kind of dedicated indicator of the artificial liquid nutritional feed quality of natural enemy insect |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2142868C (en) * | 1992-08-21 | 2001-07-24 | Yuichi Kinoshita | Chemical and microbiological test kit |
| US5955352A (en) * | 1994-12-22 | 1999-09-21 | Showa Yakuhin Kako Co., Ltd. | Instruments for chemical and microbiological tests |
-
1975
- 1975-04-22 GB GB16645/75A patent/GB1502674A/en not_active Expired
- 1975-04-23 JP JP50048733A patent/JPS50145576A/ja active Pending
- 1975-04-24 FR FR7512824A patent/FR2268863B1/fr not_active Expired
- 1975-04-24 DE DE19752518282 patent/DE2518282A1/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0017414A1 (en) * | 1979-04-02 | 1980-10-15 | FMC Corporation | Device for delivering measured quantities of reagent to an assay medium and method of assaying a liquid aqueous assay medium |
| GB2157708A (en) * | 1984-01-24 | 1985-10-30 | Eric James Sjoberg | Collecting and preserving microorganisms |
| US5494823A (en) * | 1992-01-21 | 1996-02-27 | Shimakyu Chemical Co. Ltd. | Apparatus for culturing microorganism |
| CN110463832A (en) * | 2019-08-13 | 2019-11-19 | 广州悦蜂生物防治科技有限公司 | A kind of dedicated indicator of the artificial liquid nutritional feed quality of natural enemy insect |
Also Published As
| Publication number | Publication date |
|---|---|
| DE2518282A1 (en) | 1975-10-30 |
| AU8003375A (en) | 1976-10-14 |
| FR2268863B1 (en) | 1978-02-03 |
| FR2268863A1 (en) | 1975-11-21 |
| JPS50145576A (en) | 1975-11-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed [section 19, patents act 1949] | ||
| PCNP | Patent ceased through non-payment of renewal fee |