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GB1549069A - Enzyme linked immunoassay - Google Patents

Enzyme linked immunoassay

Info

Publication number
GB1549069A
GB1549069A GB51709/76A GB5170976A GB1549069A GB 1549069 A GB1549069 A GB 1549069A GB 51709/76 A GB51709/76 A GB 51709/76A GB 5170976 A GB5170976 A GB 5170976A GB 1549069 A GB1549069 A GB 1549069A
Authority
GB
United Kingdom
Prior art keywords
antigen
enzyme linked
linked immunoassay
insoluble
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB51709/76A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Italia SRL
Original Assignee
Farmitalia Carlo Erba SRL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Farmitalia Carlo Erba SRL filed Critical Farmitalia Carlo Erba SRL
Priority to GB51709/76A priority Critical patent/GB1549069A/en
Priority to JP14733977A priority patent/JPS53101522A/en
Priority to BE183341A priority patent/BE861711A/en
Priority to CA292,743A priority patent/CA1110165A/en
Priority to BR7708181A priority patent/BR7708181A/en
Priority to SE7714034A priority patent/SE7714034L/en
Priority to HU77EA179A priority patent/HU179956B/en
Priority to DE19772755008 priority patent/DE2755008A1/en
Priority to AU31395/77A priority patent/AU517266B2/en
Priority to NZ185927A priority patent/NZ185927A/en
Priority to IT30554/77A priority patent/IT1109485B/en
Priority to IL53576A priority patent/IL53576A/en
Priority to NO774240A priority patent/NO774240L/en
Priority to NL7713692A priority patent/NL7713692A/en
Priority to GR54943A priority patent/GR63580B/en
Priority to PT67384A priority patent/PT67384B/en
Priority to FI773718A priority patent/FI773718A7/en
Priority to CH1516677A priority patent/CH628739A5/en
Priority to DK550077A priority patent/DK550077A/en
Priority to ES464929A priority patent/ES464929A1/en
Priority to AR270301A priority patent/AR226805A1/en
Priority to FR7737194A priority patent/FR2373795A1/en
Priority to TR20650A priority patent/TR20650A/en
Priority to CS778265A priority patent/CS208739B2/en
Priority to ES468934A priority patent/ES468934A1/en
Publication of GB1549069A publication Critical patent/GB1549069A/en
Priority to HK138/81A priority patent/HK13881A/en
Priority to MY367/81A priority patent/MY8100367A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

In order to determine the presence or the quantity of an antigen, an antigen (1) in insoluble form and two antibodies (2) and (3), of which the second is labelled with an enzyme, are brought into contact in the sample to be analysed. The second antibody (3) binds to the insoluble antigen (1) in a proportion which is the inverse of the quantity of antigen present in the sample. The insoluble fraction and the soluble fraction are separated and the enzymatic activity of one or the other is measured.
GB51709/76A 1976-12-10 1976-12-10 Enzyme linked immunoassay Expired GB1549069A (en)

Priority Applications (27)

Application Number Priority Date Filing Date Title
GB51709/76A GB1549069A (en) 1976-12-10 1976-12-10 Enzyme linked immunoassay
AR270301A AR226805A1 (en) 1976-12-10 1977-12-09 REAGENT TO DETERMINE THE PRESENCE OR AMOUNTS OF AN ANTIGEN T3 OR HCG IN A LIQUID SAMPLE FOR IMMUNOANALYSIS WITH LINKED ENZYMES
NL7713692A NL7713692A (en) 1976-12-10 1977-12-09 IMPROVEMENTS IN AND RELATING TO ENZYME-LINKED IMMUNO ANALYZES.
CA292,743A CA1110165A (en) 1976-12-10 1977-12-09 Double antibody immunoassay using enzyme-labelled antibody and solid phase antigen
BR7708181A BR7708181A (en) 1976-12-10 1977-12-09 ENZYME-IMMUNE-ASSESSMENT PROCESS AND SET OF MATERIALS FOR USE IN PROCESSING A PROCESS
PT67384A PT67384B (en) 1976-12-10 1977-12-09 Improvements in and relating to enzyme-linked immunoassay
HU77EA179A HU179956B (en) 1976-12-10 1977-12-09 Process for the immunological determination of antigens by means of enzyme marking
DE19772755008 DE2755008A1 (en) 1976-12-10 1977-12-09 PROCEDURE FOR IMMUNOLOGICAL DETERMINATION USING ENZYME MARKERS
AU31395/77A AU517266B2 (en) 1976-12-10 1977-12-09 Enzyme-linked immunoassay
NZ185927A NZ185927A (en) 1976-12-10 1977-12-09 Detection or assay of antigen using two antibodies one being enzyme-labelled
IT30554/77A IT1109485B (en) 1976-12-10 1977-12-09 IMPROVEMENTS RELATING TO THE IMMUNOLOGICAL TEST WITH ENZYME
IL53576A IL53576A (en) 1976-12-10 1977-12-09 Enzyme-linked immunoassay for detection of assay of antiges
NO774240A NO774240L (en) 1976-12-10 1977-12-09 PROCEDURE FOR DETECTION AND DETERMINATION OF ANTIGENES
JP14733977A JPS53101522A (en) 1976-12-10 1977-12-09 Improved enzyme binding immune analysing method
GR54943A GR63580B (en) 1976-12-10 1977-12-09 Improvements in and relating to enzyme-linked immunoassay
SE7714034A SE7714034L (en) 1976-12-10 1977-12-09 PROCEDURE FOR ENZYME COUPLE IMMUNO ANALYSIS
FI773718A FI773718A7 (en) 1976-12-10 1977-12-09 ENZYMBUNDEN IMMUNOTEST OCH MATERIALSATS FOER UTFOERANDE DAERAV
CH1516677A CH628739A5 (en) 1976-12-10 1977-12-09 Process of immunological assay using a bound enzyme, and means for implementing the process
DK550077A DK550077A (en) 1976-12-10 1977-12-09 PROCEDURE FOR ENZYMO-COUPLED IMMUNALYSIS AND THE REAGENT KIT FOR USE IN ITS EXERCISE
ES464929A ES464929A1 (en) 1976-12-10 1977-12-09 Enzyme linked immunoassay
BE183341A BE861711A (en) 1976-12-10 1977-12-09 IMMUNOLOGICAL DOSAGE BY FIXED ENZYME
FR7737194A FR2373795A1 (en) 1976-12-10 1977-12-09 IMMUNOLOGICAL DOSAGE BY FIXED ENZYME
TR20650A TR20650A (en) 1976-12-10 1977-12-09 ENZYME-MOUNTED IMMUNO DETERMINATION OR RELATED REPORT
CS778265A CS208739B2 (en) 1976-12-10 1977-12-09 Method of immunological determination of the antidotes by means of the bonded enzyme
ES468934A ES468934A1 (en) 1976-12-10 1978-04-19 Enzyme linked immunoassay
HK138/81A HK13881A (en) 1976-12-10 1981-04-09 Improvements in and relating to enzyme-linked immunoassay
MY367/81A MY8100367A (en) 1976-12-10 1981-12-30 Improvements in and relating to enzyme-linked immunoassay

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB51709/76A GB1549069A (en) 1976-12-10 1976-12-10 Enzyme linked immunoassay

Publications (1)

Publication Number Publication Date
GB1549069A true GB1549069A (en) 1979-08-01

Family

ID=10461077

Family Applications (1)

Application Number Title Priority Date Filing Date
GB51709/76A Expired GB1549069A (en) 1976-12-10 1976-12-10 Enzyme linked immunoassay

Country Status (26)

Country Link
JP (1) JPS53101522A (en)
AR (1) AR226805A1 (en)
AU (1) AU517266B2 (en)
BE (1) BE861711A (en)
BR (1) BR7708181A (en)
CA (1) CA1110165A (en)
CH (1) CH628739A5 (en)
CS (1) CS208739B2 (en)
DE (1) DE2755008A1 (en)
DK (1) DK550077A (en)
ES (2) ES464929A1 (en)
FI (1) FI773718A7 (en)
FR (1) FR2373795A1 (en)
GB (1) GB1549069A (en)
GR (1) GR63580B (en)
HK (1) HK13881A (en)
HU (1) HU179956B (en)
IL (1) IL53576A (en)
IT (1) IT1109485B (en)
MY (1) MY8100367A (en)
NL (1) NL7713692A (en)
NO (1) NO774240L (en)
NZ (1) NZ185927A (en)
PT (1) PT67384B (en)
SE (1) SE7714034L (en)
TR (1) TR20650A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0062892A1 (en) * 1981-04-13 1982-10-20 American Hoechst Corporation Single incubation immunochemical assay for creatin phosphokinase MB
EP0084531A4 (en) * 1981-07-31 1983-12-19 Sinai School Medicine Assay for viruses.
US4467031A (en) * 1981-08-21 1984-08-21 Hoffmann-La Roche Inc. Enzyme-immunoassay for carcinoembryonic antigen
US4808522A (en) * 1986-04-24 1989-02-28 Moskovsky Gosudarstvennx Universitet Imeni M. V. Lomonoso VA Enzyme immunoassays using inorganic pyrophosphatase
AT388618B (en) * 1987-05-05 1989-08-10 Binder Bernd Dr METHOD FOR QUANTITATIVE DETERMINATION OF FUNCTION AND ANTIGENT CONCENTRATION OF A SUBSTANCE CONTAINED IN A BIOLOGICAL LIQUID
EP1992636A3 (en) * 1999-11-12 2009-04-08 Amgen Inc. Process for correction of a disulfide misfold in Fc molecules

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS576363A (en) * 1980-06-13 1982-01-13 Takeda Chem Ind Ltd Production of specific antibody
CA1160566A (en) * 1980-04-25 1984-01-17 Harald Gallati Immunological determination method
JPS58151559A (en) * 1982-03-05 1983-09-08 Takeda Chem Ind Ltd Immunochemical measuring method of human villous gonadotropin and reagent therefor
US4704356A (en) * 1984-03-27 1987-11-03 Rush-Presbyterian-St. Luke's Medical Center Method of diagnosing cartilage tissue abnormalities
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
DE3807440A1 (en) * 1988-03-07 1989-09-21 Progen Biotechnik Gmbh Method for the immunological detection of substances, and a composition and a test kit
DE19508264C1 (en) * 1995-03-08 1996-02-01 Klose Werner Dipl Ing Fh Measuring contours, esp. in road surface

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (en) * 1971-02-10 1977-09-15 Organon Nv METHOD FOR THE DETERMINATION AND DETERMINATION OF SPECIFIC BINDING PROTEINS AND THEIR CORRESPONDING BINDABLE SUBSTANCES.
NL154599B (en) * 1970-12-28 1977-09-15 Organon Nv PROCEDURE FOR DETERMINING AND DETERMINING SPECIFIC BINDING PROTEINS AND THEIR CORRESPONDING BINDABLE SUBSTANCES, AND TEST PACKAGING.
US4002532A (en) * 1974-10-21 1977-01-11 Weltman Joel K Enzyme conjugates
US3951748A (en) * 1974-11-11 1976-04-20 Medical Products, Inc. Sensitized matrix for detection of disease
DK140815B (en) * 1975-06-10 1979-11-19 Weeke Bengt A method for detecting or determining antibodies in body fluids by known antigens or for determining antigens by known antibodies from body fluids and a means for use in practicing the method.

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0062892A1 (en) * 1981-04-13 1982-10-20 American Hoechst Corporation Single incubation immunochemical assay for creatin phosphokinase MB
EP0084531A4 (en) * 1981-07-31 1983-12-19 Sinai School Medicine Assay for viruses.
US4588680A (en) * 1981-07-31 1986-05-13 Mount Sinai School Of Medicine Of The City University Of New York Assay for viruses
US4467031A (en) * 1981-08-21 1984-08-21 Hoffmann-La Roche Inc. Enzyme-immunoassay for carcinoembryonic antigen
US4808522A (en) * 1986-04-24 1989-02-28 Moskovsky Gosudarstvennx Universitet Imeni M. V. Lomonoso VA Enzyme immunoassays using inorganic pyrophosphatase
AT388618B (en) * 1987-05-05 1989-08-10 Binder Bernd Dr METHOD FOR QUANTITATIVE DETERMINATION OF FUNCTION AND ANTIGENT CONCENTRATION OF A SUBSTANCE CONTAINED IN A BIOLOGICAL LIQUID
US5298401A (en) * 1987-05-05 1994-03-29 Bernd Binder Process for the quantitative determination of the function and antigenic concentration of a substance contained in a biological liquid
EP1992636A3 (en) * 1999-11-12 2009-04-08 Amgen Inc. Process for correction of a disulfide misfold in Fc molecules
US7723490B2 (en) 1999-11-12 2010-05-25 Amgen Inc. Process for correction of a disulfide misfold in Fc molecules

Also Published As

Publication number Publication date
BE861711A (en) 1978-03-31
IL53576A (en) 1981-07-31
ES464929A1 (en) 1979-01-01
SE7714034L (en) 1978-06-11
FR2373795A1 (en) 1978-07-07
CH628739A5 (en) 1982-03-15
AU517266B2 (en) 1981-07-16
IL53576A0 (en) 1978-03-10
GR63580B (en) 1979-11-22
MY8100367A (en) 1981-12-31
PT67384A (en) 1978-01-01
AU3139577A (en) 1979-06-14
HU179956B (en) 1983-01-28
DE2755008A1 (en) 1978-06-15
JPS53101522A (en) 1978-09-05
CS208739B2 (en) 1981-09-15
ES468934A1 (en) 1978-12-16
CA1110165A (en) 1981-10-06
BR7708181A (en) 1978-07-11
AR226805A1 (en) 1982-08-31
DK550077A (en) 1978-06-11
PT67384B (en) 1979-05-18
NL7713692A (en) 1978-06-13
FI773718A7 (en) 1978-06-11
NO774240L (en) 1978-06-13
IT1109485B (en) 1985-12-16
FR2373795B1 (en) 1982-09-10
TR20650A (en) 1982-03-24
HK13881A (en) 1981-04-16
NZ185927A (en) 1980-02-21

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee