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GB1414480A - Direct radioimmuno assay for antigens and their antibodies - Google Patents

Direct radioimmuno assay for antigens and their antibodies

Info

Publication number
GB1414480A
GB1414480A GB5866272A GB5866272A GB1414480A GB 1414480 A GB1414480 A GB 1414480A GB 5866272 A GB5866272 A GB 5866272A GB 5866272 A GB5866272 A GB 5866272A GB 1414480 A GB1414480 A GB 1414480A
Authority
GB
United Kingdom
Prior art keywords
antibody
antigen
coated surface
incubating
washing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB5866272A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of GB1414480A publication Critical patent/GB1414480A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/804—Radioisotope, e.g. radioimmunoassay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/82—Hepatitis associated antigens and antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Communicable Diseases (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1414480 Radioimmunoassay ABBOTT LABORATORIES 19 Dec 1972 [21 Dec 1971] 58662/72 Heading GIB A direct radioimmunoassay for determining antigen or antibody in a sample comprises contacting the sample with a coating of antigen or antibody (for determination of antibody or antigen respectively) on a solid substrate, for example the inner wall of a tube, incubating, washing the coated surface, contacting the washed coated surface with radio-actively labelled antigen or antibody (for determination of antibody or antigen respectively) incubating a second time, washing the coated surface again, counting the radiation emitted from the coated surface and comparing the result with that obtained by applying the method to a control sample known to be free from antigen or antibody. The determination of hepatitis associated antigen is described. The antibody-coated surface may be prepared by coating the inner surface of a tube with a solution containing 1 to 100 micrograms protein per ml. antibody to Australia antigen in 0.005 to 0.02 m Tris -HCl buffer containing 0.01% to 0.05% sodium azide and incubating at room temperature for from 6 to 72 hours, then washing with the same buffer solution.
GB5866272A 1971-12-21 1972-12-19 Direct radioimmuno assay for antigens and their antibodies Expired GB1414480A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US210510A US3867517A (en) 1971-12-21 1971-12-21 Direct radioimmunoassay for antigens and their antibodies

Publications (1)

Publication Number Publication Date
GB1414480A true GB1414480A (en) 1975-11-19

Family

ID=22783190

Family Applications (1)

Application Number Title Priority Date Filing Date
GB5866272A Expired GB1414480A (en) 1971-12-21 1972-12-19 Direct radioimmuno assay for antigens and their antibodies

Country Status (13)

Country Link
US (1) US3867517A (en)
AT (1) AT326818B (en)
AU (1) AU470050B2 (en)
BE (1) BE793061A (en)
CA (1) CA988420A (en)
CH (2) CH589445A5 (en)
DE (1) DE2262413B2 (en)
FR (1) FR2164705B1 (en)
GB (1) GB1414480A (en)
IT (1) IT988084B (en)
NL (1) NL7217371A (en)
PH (1) PH13655A (en)
ZA (1) ZA728798B (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4289748A (en) * 1979-05-31 1981-09-15 United States Of America Ultrasensitive enzymatic radioimmunoassay method
EP0154902A3 (en) * 1984-03-07 1987-12-16 New York Blood Center, Inc. Pre-s gene coded peptide hepatitis b immunogens, vaccines, diagnostics, and synthetic lipid vesicle carriers
US4861588A (en) * 1985-02-05 1989-08-29 New York Blood Center, Inc. Pre-S gene coded peptide hepatitis B immunogens, vaccines, diagnostics, and synthetic lipid vesicle carriers
US5158769A (en) * 1984-03-07 1992-10-27 New York Blood Center, Inc. Pre-S gene coded peptide hepatitis B immunogens, vaccines, diagnostics, and synthetic lipid vesicle carriers
US5204096A (en) * 1984-03-07 1993-04-20 New York Blood Center, Inc. Pre-S gene coded peptide hepatitis B immunogens, vaccines, diagnostics, and synthetic lipid vesicle carriers

Families Citing this family (879)

* Cited by examiner, † Cited by third party
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US4012494A (en) * 1971-12-21 1977-03-15 Abbott Laboratories Direct radioimmunoassay for antigens and their antibodies
US3949064A (en) * 1973-10-26 1976-04-06 Baxter Laboratories, Inc. Method of detecting antigens or antibodies
US4172117A (en) * 1974-05-20 1979-10-23 Biotest-Serum-Institut Gmbh Method for the simultaneous measurement of antigens and their antibodies by solid-phase radioimmunoassay
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US3646346A (en) * 1968-12-26 1972-02-29 Pharmacia Ab Antibody-coated tube system for radioimmunoassay

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US4289748A (en) * 1979-05-31 1981-09-15 United States Of America Ultrasensitive enzymatic radioimmunoassay method
EP0154902A3 (en) * 1984-03-07 1987-12-16 New York Blood Center, Inc. Pre-s gene coded peptide hepatitis b immunogens, vaccines, diagnostics, and synthetic lipid vesicle carriers
US5158769A (en) * 1984-03-07 1992-10-27 New York Blood Center, Inc. Pre-S gene coded peptide hepatitis B immunogens, vaccines, diagnostics, and synthetic lipid vesicle carriers
US5204096A (en) * 1984-03-07 1993-04-20 New York Blood Center, Inc. Pre-S gene coded peptide hepatitis B immunogens, vaccines, diagnostics, and synthetic lipid vesicle carriers
US5620844A (en) * 1984-03-07 1997-04-15 New York Blood, Inc. Assays for detecting hepatitis B virus envelope antigens or antibodies thereto and diagnostic test kits for use in performing the assays
US4861588A (en) * 1985-02-05 1989-08-29 New York Blood Center, Inc. Pre-S gene coded peptide hepatitis B immunogens, vaccines, diagnostics, and synthetic lipid vesicle carriers

Also Published As

Publication number Publication date
CH589445A5 (en) 1977-07-15
PH13655A (en) 1980-08-21
IT988084B (en) 1975-04-10
DE2262413B2 (en) 1977-11-17
NL7217371A (en) 1973-06-25
US3867517A (en) 1975-02-18
AT326818B (en) 1975-12-29
FR2164705A1 (en) 1973-08-03
CH568061A5 (en) 1975-10-31
ATA1088772A (en) 1975-03-15
DE2262413A1 (en) 1973-07-05
ZA728798B (en) 1974-02-27
FR2164705B1 (en) 1976-10-29
CA988420A (en) 1976-05-04
AU470050B2 (en) 1974-06-20
AU5021472A (en) 1974-06-20
BE793061A (en) 1973-06-20

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414F Notice of opposition given (sect. 14/1949)
414A Case decided by the comptroller ** specification amended (sect. 14/1949)
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PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee