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GB1404628A - Method and apparatus for analyzing microscopic particles - Google Patents

Method and apparatus for analyzing microscopic particles

Info

Publication number
GB1404628A
GB1404628A GB5644672A GB5644672A GB1404628A GB 1404628 A GB1404628 A GB 1404628A GB 5644672 A GB5644672 A GB 5644672A GB 5644672 A GB5644672 A GB 5644672A GB 1404628 A GB1404628 A GB 1404628A
Authority
GB
United Kingdom
Prior art keywords
flash
particles
platelets
vidicon
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB5644672A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coulter Electronics Inc
Original Assignee
Coulter Electronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Coulter Electronics Inc filed Critical Coulter Electronics Inc
Priority to GB5644672A priority Critical patent/GB1404628A/en
Publication of GB1404628A publication Critical patent/GB1404628A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1404628 Photo-electric particle analyzers COULTER ELECTRONICS Inc 7 Dec 1972 56446/72 Heading G1A Microscopic particles are analyzed by illuminating them with a sequence of pulses of EM radiation and separately scanning the image produced by each pulse to produce output data, the system being arranged so that data relating to different parameters of the particules are obtained by successive pulses. In a first embodiment, Fig. 1, three flash tubes 22, 24, 26 are arranged with a pair of dichroic mirrors 32, 34 to trans-illuminate particles carried on a microscope slide 16. The image magnified by the microscope 10 is viewed by a vidicon camera 12 which is preferably of the storage type. The target of the vidicon is scanned with a raster and is provided with vertical blanking pulses which are also applied via shaping circuitry to a ring counter which triggers the flash tubes in turn, Fig. 3 (not shown). This results in the sample being illuminated by a sequence of flashes of red, blue and green light each flash occuring in the blanking intervals between successive scans. The output of the vidicon and signals from the scanning circuits and flash tube control circuits are applied to a signal processor, which is provided with a store to enable the parameters of the particles to be determined and hence the particles to be identified and counted. Instead of trans-illumination the flash tubes may be arranged for reflective illumination. The light from the flash tubes may be individually filtered, the dichroic mirrors being replaced by half silvered mirrors. A single flash tube may be used together with a synchronously rotated filtered wheel placed in front of the camera. The illumination may also consist of IR or UV radiation. The slide may be moved continuously or stepwise during the measurements. In a specific application the sample is blood stained with Wright's stain. With green light the white cell nuclei and platelets stand out in sharp contrast and can be differential by, their sizes and shapes. With blue light the white cell nuclei cannot be distinguished from the cytoplasm, the white and red cells being equally opaque while the platelets are transparent. These parameters can be utilized to determine the numbers of white cells, red cells and platelets in the sample.
GB5644672A 1972-12-07 1972-12-07 Method and apparatus for analyzing microscopic particles Expired GB1404628A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB5644672A GB1404628A (en) 1972-12-07 1972-12-07 Method and apparatus for analyzing microscopic particles

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB5644672A GB1404628A (en) 1972-12-07 1972-12-07 Method and apparatus for analyzing microscopic particles

Publications (1)

Publication Number Publication Date
GB1404628A true GB1404628A (en) 1975-09-03

Family

ID=10476644

Family Applications (1)

Application Number Title Priority Date Filing Date
GB5644672A Expired GB1404628A (en) 1972-12-07 1972-12-07 Method and apparatus for analyzing microscopic particles

Country Status (1)

Country Link
GB (1) GB1404628A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2152660A (en) * 1983-11-28 1985-08-07 Cii Analysis of biological products
EP0501005A3 (en) * 1991-02-27 1992-12-09 Toa Medical Electronics Co., Ltd. Flow imaging cytometer
WO2002056084A1 (en) * 2001-01-11 2002-07-18 Interscope Technologies, Inc. A system for microscopic digital imaging using a pulse light illumination system
GB2383487A (en) * 2001-12-18 2003-06-25 Fairfield Imaging Ltd Digital microscope with continuously moving stage
US7155049B2 (en) 2001-01-11 2006-12-26 Trestle Acquisition Corp. System for creating microscopic digital montage images
US7212660B2 (en) 2001-01-11 2007-05-01 Clarient, Inc. System and method for finding regions of interest for microscopic digital montage imaging

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2152660A (en) * 1983-11-28 1985-08-07 Cii Analysis of biological products
EP0501005A3 (en) * 1991-02-27 1992-12-09 Toa Medical Electronics Co., Ltd. Flow imaging cytometer
US5247339A (en) * 1991-02-27 1993-09-21 Toa Medical Electronics Co., Ltd. Flow imaging cytometer
US7421102B2 (en) 2001-01-11 2008-09-02 Carl Zeiss Microimaging Ais, Inc. System and method for finding regions of interest for microscopic digital montage imaging
WO2002056084A1 (en) * 2001-01-11 2002-07-18 Interscope Technologies, Inc. A system for microscopic digital imaging using a pulse light illumination system
US7876948B2 (en) 2001-01-11 2011-01-25 Carl Zeiss Microimaging Gmbh System for creating microscopic digital montage images
US6798571B2 (en) 2001-01-11 2004-09-28 Interscope Technologies, Inc. System for microscopic digital montage imaging using a pulse light illumination system
US7155049B2 (en) 2001-01-11 2006-12-26 Trestle Acquisition Corp. System for creating microscopic digital montage images
US7212660B2 (en) 2001-01-11 2007-05-01 Clarient, Inc. System and method for finding regions of interest for microscopic digital montage imaging
US7869641B2 (en) 2001-01-11 2011-01-11 Carl Zeiss Microimaging Gmbh System and method for finding regions of interest for microscopic digital montage imaging
GB2383487B (en) * 2001-12-18 2006-09-27 Fairfield Imaging Ltd Method and apparatus for acquiring digital microscope images
US7388599B2 (en) 2001-12-18 2008-06-17 Medical Solutions Plc Method and apparatus for acquiring digital microscope images
GB2383487A (en) * 2001-12-18 2003-06-25 Fairfield Imaging Ltd Digital microscope with continuously moving stage
US8253788B2 (en) 2001-12-18 2012-08-28 Source Bioscience Plc Method and apparatus for acquiring digital microscope images
US8749627B2 (en) 2001-12-18 2014-06-10 Source Bioscience Plc Method and apparatus for acquiring digital microscope images

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee