GB1333420A - Reagent combination for use in the quantitative determination of amylase in body fluids - Google Patents
Reagent combination for use in the quantitative determination of amylase in body fluidsInfo
- Publication number
- GB1333420A GB1333420A GB1333420DA GB1333420A GB 1333420 A GB1333420 A GB 1333420A GB 1333420D A GB1333420D A GB 1333420DA GB 1333420 A GB1333420 A GB 1333420A
- Authority
- GB
- United Kingdom
- Prior art keywords
- amylase
- reagent
- incubated
- surfactant
- serum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 6
- 239000004382 Amylase Substances 0.000 title abstract 4
- 102000013142 Amylases Human genes 0.000 title abstract 4
- 108010065511 Amylases Proteins 0.000 title abstract 4
- 235000019418 amylase Nutrition 0.000 title abstract 4
- 210000001124 body fluid Anatomy 0.000 title abstract 2
- 239000010839 body fluid Substances 0.000 title abstract 2
- WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 abstract 6
- 239000000758 substrate Substances 0.000 abstract 4
- 229920002472 Starch Polymers 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 3
- 239000008107 starch Substances 0.000 abstract 3
- 239000004094 surface-active agent Substances 0.000 abstract 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- CBPYOHALYYGNOE-UHFFFAOYSA-M potassium;3,5-dinitrobenzoate Chemical compound [K+].[O-]C(=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1 CBPYOHALYYGNOE-UHFFFAOYSA-M 0.000 abstract 2
- 210000002966 serum Anatomy 0.000 abstract 2
- 235000019698 starch Nutrition 0.000 abstract 2
- -1 (E) Substances 0.000 abstract 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 abstract 1
- 229910052802 copper Inorganic materials 0.000 abstract 1
- 239000010949 copper Substances 0.000 abstract 1
- 230000000415 inactivating effect Effects 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- 235000011164 potassium chloride Nutrition 0.000 abstract 1
- 239000001103 potassium chloride Substances 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- XMVONEAAOPAGAO-UHFFFAOYSA-N sodium tungstate Chemical compound [Na+].[Na+].[O-][W]([O-])(=O)=O XMVONEAAOPAGAO-UHFFFAOYSA-N 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 125000003831 tetrazolyl group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
1333420 Determination of amylase XEROX CORP 1 Dec 1970 56964/70 Heading B1X Amylase is determined in body fluids using a reagent combination comprising (A) a tetrazolium salt and (B) an alkaline buffer capable of inactivating amylase in a serum-starch substrate solution. The reagent used in the test comprise a composition comprising soluble starch as substrate (C), a buffer (D) to maintain the sample-substrate solution at pH 6 to 8, and an amylaseactivating substance (E) which may be sodium chloride or potassium chloride, together with the reagent combination as above. The alkaline buffer (B) may comprise diethylamine hydrochloride and lithium hydroxide in proportions to give a pH of at least 10. The reagent may be in the form of three tablets, comprising (C), (D), (E) and surfactant; lithium hydroxide, (E), and surfactant; diethylamine hydrochloride, (A), (E) and surfactant. In one method of carrying out the test, a serum sample is incubated with the first tablet, the second and third tablets added, and incubation continued. Optical density is then read periodically at 560 mu. There is reference to a conventional procedure wherein serum is incubated with starch substrate solution, the mixture is acidified and sodium tungstate solution added then an alkaline copper solution, the mixture is incubated and an arsenomolybdate colour forming reagent added.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB5696470 | 1970-12-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1333420A true GB1333420A (en) | 1973-10-10 |
Family
ID=10477997
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1333420D Expired GB1333420A (en) | 1970-12-01 | 1970-12-01 | Reagent combination for use in the quantitative determination of amylase in body fluids |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB1333420A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4337310A (en) | 1978-05-02 | 1982-06-29 | Boehringer Mannheim Gmbh | Method for the determination of α-amylase |
-
1970
- 1970-12-01 GB GB1333420D patent/GB1333420A/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4337310A (en) | 1978-05-02 | 1982-06-29 | Boehringer Mannheim Gmbh | Method for the determination of α-amylase |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PCNP | Patent ceased through non-payment of renewal fee |