GB1318568A - Colourimetric assay of dehydrogenases - Google Patents
Colourimetric assay of dehydrogenasesInfo
- Publication number
- GB1318568A GB1318568A GB4449870A GB1318568DA GB1318568A GB 1318568 A GB1318568 A GB 1318568A GB 4449870 A GB4449870 A GB 4449870A GB 1318568D A GB1318568D A GB 1318568DA GB 1318568 A GB1318568 A GB 1318568A
- Authority
- GB
- United Kingdom
- Prior art keywords
- dehydrogenase
- composition
- phosphate
- enzyme
- glucose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108020005199 Dehydrogenases Proteins 0.000 title abstract 2
- 238000003556 assay Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 abstract 3
- 101710088194 Dehydrogenase Proteins 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract 2
- CCBICDLNWJRFPO-UHFFFAOYSA-N 2,6-dichloroindophenol Chemical compound C1=CC(O)=CC=C1N=C1C=C(Cl)C(=O)C(Cl)=C1 CCBICDLNWJRFPO-UHFFFAOYSA-N 0.000 abstract 1
- BIRSGZKFKXLSJQ-SQOUGZDYSA-N 6-Phospho-D-gluconate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O BIRSGZKFKXLSJQ-SQOUGZDYSA-N 0.000 abstract 1
- 102000004567 6-phosphogluconate dehydrogenase Human genes 0.000 abstract 1
- 108020001657 6-phosphogluconate dehydrogenase Proteins 0.000 abstract 1
- 102100031126 6-phosphogluconolactonase Human genes 0.000 abstract 1
- 108010029731 6-phosphogluconolactonase Proteins 0.000 abstract 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 abstract 1
- NBSCHQHZLSJFNQ-GASJEMHNSA-N D-Glucose 6-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@H]1O NBSCHQHZLSJFNQ-GASJEMHNSA-N 0.000 abstract 1
- VFRROHXSMXFLSN-UHFFFAOYSA-N Glc6P Natural products OP(=O)(O)OCC(O)C(O)C(O)C(O)C=O VFRROHXSMXFLSN-UHFFFAOYSA-N 0.000 abstract 1
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 abstract 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 abstract 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 abstract 1
- XJLXINKUBYWONI-NNYOXOHSSA-N NADP zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-NNYOXOHSSA-N 0.000 abstract 1
- HWYNRVXFYFQSID-UHFFFAOYSA-M benzo[a]phenoxazin-9-ylidene(dimethyl)azanium;chloride Chemical compound [Cl-].C1=CC=C2C(N=C3C=CC(C=C3O3)=[N+](C)C)=C3C=CC2=C1 HWYNRVXFYFQSID-UHFFFAOYSA-M 0.000 abstract 1
- 239000008366 buffered solution Substances 0.000 abstract 1
- 229910052739 hydrogen Inorganic materials 0.000 abstract 1
- 239000001257 hydrogen Substances 0.000 abstract 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 abstract 1
- 235000014655 lactic acid Nutrition 0.000 abstract 1
- 239000004310 lactic acid Substances 0.000 abstract 1
- 229950006238 nadide Drugs 0.000 abstract 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 abstract 1
- 239000004745 nonwoven fabric Substances 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- 239000004033 plastic Substances 0.000 abstract 1
- 229920003023 plastic Polymers 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 239000002023 wood Substances 0.000 abstract 1
- 239000002759 woven fabric Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/805—Test papers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
1318568 Determination of dehydrogenases MILES LABORATORIES Inc 17 Sept 1970 44498/70 Heading B1X A composition for the assay of a dehydrogenase enzyme comprises nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate, a substrate for the enzyme, a terminal acceptor dye and meldola's blue as intermediary hydrogen carrier. The terminal acceptor dye may be 2-(P iodophenyl)-3 (pnitrophenyl)-5-phenyl tetrazolium chloride, bromide or iodide or 2,6-dichloroindophenol. The substrate-enzyme pair may be lactic acid, lactic dehydrogenase; glucose 6-phosphate, glucose-6-phosphate dehydrogenase; 6-phosphogluconate, 6-phosphogluconate dehydrogenase. The composition may be used in aqueous buffered solution or in or on a carrier which may be filter paper thickness 0.01 to 0.2", wood strip, synthetic plastics or woven or non-woven fabric. In use, the composition in solution and the sample are mixed and the rate of change of optical density at 500 mu observed.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB4449870 | 1970-09-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1318568A true GB1318568A (en) | 1973-05-31 |
Family
ID=10433592
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB4449870A Expired GB1318568A (en) | 1970-09-17 | 1970-09-17 | Colourimetric assay of dehydrogenases |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US3732147A (en) |
| CA (1) | CA955160A (en) |
| GB (1) | GB1318568A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2147415A (en) * | 1983-08-05 | 1985-05-09 | Powell & Scholefield Limited | Device and method for antibiotic sensitivity testing |
| EP0244825A1 (en) * | 1986-05-09 | 1987-11-11 | Fuji Photo Film Co., Ltd. | Dry-type analytical element for cholesterol |
| FR2618905A1 (en) * | 1987-07-27 | 1989-02-03 | Suekami Pharmaceuti Research | METHOD FOR DETERMINING THE PRESENCE OF LIVE SPERMATOZOIDS AND TESTING THEREFOR |
| US6051393A (en) * | 1994-03-29 | 2000-04-18 | Jones; Sonja K. | Method of detecting malignant and pre-malignant conditions of the cervix, and test kits therefor |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3852034A (en) * | 1972-06-21 | 1974-12-03 | American Sterilizer Co | Indicator for effectiveness of ethylene oxide sterilization |
| US4056485A (en) * | 1974-10-04 | 1977-11-01 | Warner-Lambert Company | Stable colored reference standard for enzymatic determinations |
| US4006061A (en) * | 1975-12-29 | 1977-02-01 | Monsanto Company | Lactate dehydrogenase determination method |
| US4223799A (en) * | 1979-06-11 | 1980-09-23 | Time Saving Falls, Inc. | Releasable locking means for closure caps |
| DE3048662A1 (en) * | 1980-12-23 | 1982-07-22 | Boehringer Mannheim Gmbh, 6800 Mannheim | STABILIZED PREPARATION OF TETRAZOLIUM SALTS |
| DE3247894A1 (en) * | 1982-12-24 | 1984-06-28 | Merck Patent Gmbh, 6100 Darmstadt | TEST SYSTEM AND METHOD FOR DETERMINING NAD (P) H |
| US4728608A (en) * | 1985-04-08 | 1988-03-01 | Cambridge Bioscience Corporation | Bacterial screening system with enhanced shelf life |
| DE69327242T2 (en) * | 1992-07-02 | 2000-05-25 | Roche Diagnostics Corp., Indianapolis | STABILIZATION OF TETRAZOLIUM SALTS IN A REAGENT |
| DE10001529A1 (en) * | 2000-01-15 | 2001-07-19 | Roche Diagnostics Gmbh | Stabilized coenzyme solutions and their use for the determination of dehydrogenases or their substrates |
| TWI275795B (en) * | 2001-02-14 | 2007-03-11 | Sysmex Corp | Novel assay method |
| TW201441620A (en) * | 2013-04-19 | 2014-11-01 | Nat Univ Tsing Hua | Xylem fiber device for detection |
| TWI490475B (en) * | 2013-06-25 | 2015-07-01 | Nat Univ Tsing Hua | Three dimensional lignocellulosic detection device |
| CN105527279A (en) * | 2014-09-30 | 2016-04-27 | 郑兆珉 | Food safety detection device and manufacturing method thereof |
| TWI561819B (en) * | 2014-10-31 | 2016-12-11 | Univ Nat Tsing Hua | Three dimensional detection device and manufacturing method for the same |
-
1970
- 1970-09-17 GB GB4449870A patent/GB1318568A/en not_active Expired
-
1971
- 1971-09-16 CA CA123,014A patent/CA955160A/en not_active Expired
- 1971-09-16 US US00181192A patent/US3732147A/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2147415A (en) * | 1983-08-05 | 1985-05-09 | Powell & Scholefield Limited | Device and method for antibiotic sensitivity testing |
| EP0244825A1 (en) * | 1986-05-09 | 1987-11-11 | Fuji Photo Film Co., Ltd. | Dry-type analytical element for cholesterol |
| FR2618905A1 (en) * | 1987-07-27 | 1989-02-03 | Suekami Pharmaceuti Research | METHOD FOR DETERMINING THE PRESENCE OF LIVE SPERMATOZOIDS AND TESTING THEREFOR |
| EP0302765A1 (en) * | 1987-07-27 | 1989-02-08 | Clonatec | Method for detecting living spermatozoa, and tests therefor |
| US6051393A (en) * | 1994-03-29 | 2000-04-18 | Jones; Sonja K. | Method of detecting malignant and pre-malignant conditions of the cervix, and test kits therefor |
Also Published As
| Publication number | Publication date |
|---|---|
| CA955160A (en) | 1974-09-24 |
| US3732147A (en) | 1973-05-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed [section 19, patents act 1949] | ||
| PLNP | Patent lapsed through nonpayment of renewal fees |