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GB1386303A - Method and apparatus for lymphocyte separation from blood - Google Patents

Method and apparatus for lymphocyte separation from blood

Info

Publication number
GB1386303A
GB1386303A GB1684972A GB1684972A GB1386303A GB 1386303 A GB1386303 A GB 1386303A GB 1684972 A GB1684972 A GB 1684972A GB 1684972 A GB1684972 A GB 1684972A GB 1386303 A GB1386303 A GB 1386303A
Authority
GB
United Kingdom
Prior art keywords
tube
container
lymphocytes
frame
separator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1684972A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Technicon Instruments Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technicon Instruments Corp filed Critical Technicon Instruments Corp
Publication of GB1386303A publication Critical patent/GB1386303A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • External Artificial Organs (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

1386303 Magnetic separators TECHNICON INSTRUMENTS CORP 12 April 1972 [13 April 1971] 16849/72 Heading B2J [Also in Division A5] Apparatus for the separation of lymphocytes from whole blood samples comprises a frame 46 having clamps 48 and guides 50 for supporting a hypodermic syringe 10 containing a blood sample in which leucocytes have been tagged with magnetic particles; a container 90 for the separated lymphocytes: a separator constituted by a magnet (58) with pole pieces (64), (66) Figs. 7 and 8 (not shown); and a transfer tube 74 which passes from the frame 46, through the separator and back to the frame 46, and which has sealed ends 76, 78 held in supports 92, 82 adjacent the upper ends of the container 90 and the syringe 10 respectively. In use, a drive mechanism 52 working in a slot 54 urges the plunger 14 upwardly so that the needle of the syringe 10 punctures the tube 74, the other end of which has also been punctured by a hypodermic needle 96 communicating with the container 90. As the plunger 14 continues to move upwardly blood is forced through the tube 74 and the flowing stream passes through the separator, where the magnetic field acts to hold the magnetically tagged leucocytes against the bottom of the tube yet allows the lymphocytes to flow through the tube for collection in container 90.
GB1684972A 1971-04-13 1972-04-12 Method and apparatus for lymphocyte separation from blood Expired GB1386303A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13352471A 1971-04-13 1971-04-13

Publications (1)

Publication Number Publication Date
GB1386303A true GB1386303A (en) 1975-03-05

Family

ID=22459022

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1684972A Expired GB1386303A (en) 1971-04-13 1972-04-12 Method and apparatus for lymphocyte separation from blood

Country Status (12)

Country Link
US (1) US3709791A (en)
JP (1) JPS5522445B1 (en)
BE (1) BE781532A (en)
CA (1) CA955892A (en)
CH (1) CH544308A (en)
DE (1) DE2217266C2 (en)
FR (1) FR2132800B1 (en)
GB (1) GB1386303A (en)
IT (1) IT968389B (en)
NL (1) NL170983C (en)
SE (1) SE378309B (en)
SU (1) SU433695A3 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4855045A (en) * 1982-01-14 1989-08-08 Reed Thomas A Method and apparatus for the separation of organic substances from a suspension or solution

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE377811B (en) * 1973-11-13 1975-07-28 S E Lindgren
DE2656317C2 (en) * 1976-12-11 1986-06-19 Kernforschungsanlage Jülich GmbH, 5170 Jülich Process for the preparation of a suspension of loaded erythrocytes
SE431214B (en) * 1977-06-02 1984-01-23 Klaus H Mosbach SET TO MAKE MAGNETIC POLYMER PARTICLES BASED ON A PREFERRED BIOLOGY ACTIVE SUBSTANCE
US4224942A (en) * 1979-04-25 1980-09-30 Northwestern University Cell fractionating method
DE3009126A1 (en) * 1980-03-10 1981-10-08 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen METHOD FOR OBTAINING FUNCTIONAL AND MORPHOLOGICALLY INTACT AND VIABLE LEUCOCYTES FROM BLOOD
US4508625A (en) * 1982-10-18 1985-04-02 Graham Marshall D Magnetic separation using chelated magnetic ions
US4487700A (en) * 1983-02-18 1984-12-11 Technicon Instruments Corporation Method and apparatus for separating lymphocytes from anticoagulated blood
DE3321238C2 (en) * 1983-06-11 1985-05-02 Kernforschungsanlage Jülich GmbH, 5170 Jülich Method and device for the fusion of cells
US4664796A (en) * 1985-09-16 1987-05-12 Coulter Electronics, Inc. Flux diverting flow chamber for high gradient magnetic separation of particles from a liquid medium
US6013531A (en) * 1987-10-26 2000-01-11 Dade International Inc. Method to use fluorescent magnetic polymer particles as markers in an immunoassay
US20030203491A1 (en) * 2002-04-26 2003-10-30 Andrevski Zygmunt M. Gravitational flow purification system
US7867765B2 (en) 2005-12-28 2011-01-11 The General Hospital Corporation Blood cell sorting methods and systems
EP2306959A2 (en) * 2008-07-11 2011-04-13 The General Hospital Corporation Magnetic apparatus for blood separation
CN110904040A (en) * 2019-11-18 2020-03-24 江苏久腾医学科技有限公司 Cell stripping method

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3700555A (en) * 1970-10-12 1972-10-24 Technicon Instr Method and apparatus for lymphocyte separation from blood

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4855045A (en) * 1982-01-14 1989-08-08 Reed Thomas A Method and apparatus for the separation of organic substances from a suspension or solution

Also Published As

Publication number Publication date
IT968389B (en) 1974-03-20
NL7204570A (en) 1972-10-17
FR2132800B1 (en) 1975-10-24
BE781532A (en) 1972-10-02
CH544308A (en) 1973-11-15
DE2217266A1 (en) 1972-10-26
SU433695A3 (en) 1974-06-25
CA955892A (en) 1974-10-08
US3709791A (en) 1973-01-09
SE378309B (en) 1975-08-25
NL170983C (en) 1983-01-17
JPS5522445B1 (en) 1980-06-17
FR2132800A1 (en) 1972-11-24
DE2217266C2 (en) 1984-01-19
NL170983B (en) 1982-08-16

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee