GB1293563A - Enzymes and methods for their preparation, isolation and purification - Google Patents
Enzymes and methods for their preparation, isolation and purificationInfo
- Publication number
- GB1293563A GB1293563A GB917970A GB917970A GB1293563A GB 1293563 A GB1293563 A GB 1293563A GB 917970 A GB917970 A GB 917970A GB 917970 A GB917970 A GB 917970A GB 1293563 A GB1293563 A GB 1293563A
- Authority
- GB
- United Kingdom
- Prior art keywords
- mixture
- enzyme
- proteolytic
- enzyme mixture
- proteins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108090000790 Enzymes Proteins 0.000 title abstract 7
- 102000004190 Enzymes Human genes 0.000 title abstract 7
- 238000002955 isolation Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 238000002360 preparation method Methods 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 229940088598 enzyme Drugs 0.000 abstract 6
- 239000000203 mixture Substances 0.000 abstract 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 2
- 239000005018 casein Substances 0.000 abstract 2
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 abstract 2
- 235000021240 caseins Nutrition 0.000 abstract 2
- 230000003301 hydrolyzing effect Effects 0.000 abstract 2
- 235000018102 proteins Nutrition 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 230000002797 proteolythic effect Effects 0.000 abstract 2
- JVIPLYCGEZUBIO-UHFFFAOYSA-N 2-(4-fluorophenyl)-1,3-dioxoisoindole-5-carboxylic acid Chemical compound O=C1C2=CC(C(=O)O)=CC=C2C(=O)N1C1=CC=C(F)C=C1 JVIPLYCGEZUBIO-UHFFFAOYSA-N 0.000 abstract 1
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 abstract 1
- 239000005695 Ammonium acetate Substances 0.000 abstract 1
- 102000013142 Amylases Human genes 0.000 abstract 1
- 108010065511 Amylases Proteins 0.000 abstract 1
- 229920002307 Dextran Polymers 0.000 abstract 1
- 229920001425 Diethylaminoethyl cellulose Polymers 0.000 abstract 1
- 108090000371 Esterases Proteins 0.000 abstract 1
- 239000001828 Gelatine Substances 0.000 abstract 1
- 108090001060 Lipase Proteins 0.000 abstract 1
- 102000004882 Lipase Human genes 0.000 abstract 1
- 239000004367 Lipase Substances 0.000 abstract 1
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 102000035195 Peptidases Human genes 0.000 abstract 1
- 241000187436 Streptomyces globisporus Species 0.000 abstract 1
- 241001342522 Vampyrum spectrum Species 0.000 abstract 1
- 229940043376 ammonium acetate Drugs 0.000 abstract 1
- 235000019257 ammonium acetate Nutrition 0.000 abstract 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 1
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 1
- 239000001166 ammonium sulphate Substances 0.000 abstract 1
- 235000019418 amylase Nutrition 0.000 abstract 1
- 229940025131 amylases Drugs 0.000 abstract 1
- -1 aromatic amino acids Chemical class 0.000 abstract 1
- 229920002678 cellulose Polymers 0.000 abstract 1
- 239000001913 cellulose Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000005194 fractionation Methods 0.000 abstract 1
- 229920000159 gelatin Polymers 0.000 abstract 1
- 235000019322 gelatine Nutrition 0.000 abstract 1
- 238000002523 gelfiltration Methods 0.000 abstract 1
- 102000034238 globular proteins Human genes 0.000 abstract 1
- 108091005896 globular proteins Proteins 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 235000019421 lipase Nutrition 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Gastroenterology & Hepatology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Detergent Compositions (AREA)
- Peptides Or Proteins (AREA)
Abstract
1293563 Hydrolytic enzyme mixture ASTRA LAKEMEDEL AB 6 April 1970 [10 April 1969 25 Feb 1970] 18582/69 and 9179/70 Heading C3H A stable hydrolytic enzyme mixture which is a mixture of proteins is characterized in that it (a) has a U.V. spectrum characteristic of proteins containing aromatic amino acids, (b) is proteolytically active against casein, haemoglobin and gelatine, with a proteolytic optimum at pH 9-10 with casein, (c) hydrolyses N-α- tosyl-L-arginine ethyl ester, (d) contains proteolytic activity inducable by heating, (e) also contains esterases, lipases and amylases, (f) precipitates a heat stable enzyme with organic solvents (e.g. acetone) or ammonium sulphate, and (g) has stability and activity independent of Ca<SP>++</SP> ion concentration in solution. The mixture may be formed by fermenting Streptomyces globisporus variant NRRL 3762 under submerged aerobic conditions in a nutrient medium at, e.g. 26-30‹ C. and an initial pH of 6À0-7À5. A proteolytic enzyme fraction may be obtained from the enzyme mixture by subjecting it to gel filtration using a modified dextran having a fractionation range for peptides and globular proteins of molecular weight 1500-30,000 and eluting the dextran with water, or by contacting a solution of the enzymes with diethylaminoethyl cellulose and eluting the cellulose with ammonium acetate at pH 7À4, or by subjecting the enzyme mixture to isoelectric separation and recovering the fraction eluted at pH 4-6.
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI700770A FI47779C (en) | 1969-04-10 | 1970-03-19 | Method for preparing a hydrolytic enzyme mixture. |
| FI00770/70A FI47779B (en) | 1969-04-10 | 1970-03-19 | |
| SE04417/70A SE362252B (en) | 1969-04-10 | 1970-03-31 | |
| BE748297D BE748297A (en) | 1969-04-10 | 1970-04-01 | ENZYMES AND METHOD FOR THE PREPARATION, ISOLATION AND PURIFICATION OF THESE ENZYMES |
| US25454A US3684658A (en) | 1969-04-10 | 1970-04-03 | Enzymes and process for their preparation |
| AU13462/70A AU1346270A (en) | 1969-04-10 | 1970-04-06 | Enzymes and methods for their preparation, isolation and purification |
| DE19702016822 DE2016822A1 (en) | 1969-04-10 | 1970-04-09 | Enzyme mixture and process for its preparation |
| NL7005169A NL7005169A (en) | 1969-04-10 | 1970-04-10 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1858269 | 1969-04-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1293563A true GB1293563A (en) | 1972-10-18 |
Family
ID=10114925
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB917970A Expired GB1293563A (en) | 1969-04-10 | 1970-02-25 | Enzymes and methods for their preparation, isolation and purification |
Country Status (2)
| Country | Link |
|---|---|
| FR (1) | FR2054547A1 (en) |
| GB (1) | GB1293563A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56106593A (en) * | 1980-01-24 | 1981-08-24 | Dainippon Pharmaceut Co Ltd | Alkali protease m3 |
-
1970
- 1970-02-25 GB GB917970A patent/GB1293563A/en not_active Expired
- 1970-04-09 FR FR7012868A patent/FR2054547A1/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| FR2054547B1 (en) | 1973-03-16 |
| FR2054547A1 (en) | 1971-04-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PLNP | Patent lapsed through nonpayment of renewal fees |