GB1286857A - Test kit for determining virus antibody - Google Patents
Test kit for determining virus antibodyInfo
- Publication number
- GB1286857A GB1286857A GB5304069A GB5304069A GB1286857A GB 1286857 A GB1286857 A GB 1286857A GB 5304069 A GB5304069 A GB 5304069A GB 5304069 A GB5304069 A GB 5304069A GB 1286857 A GB1286857 A GB 1286857A
- Authority
- GB
- United Kingdom
- Prior art keywords
- buffer
- cells
- serum
- test
- fixed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 241000700605 Viruses Species 0.000 title abstract 3
- 239000000872 buffer Substances 0.000 abstract 5
- 210000002966 serum Anatomy 0.000 abstract 5
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 abstract 3
- 230000002745 absorbent Effects 0.000 abstract 3
- 239000002250 absorbent Substances 0.000 abstract 3
- 229910052783 alkali metal Inorganic materials 0.000 abstract 3
- 150000001340 alkali metals Chemical class 0.000 abstract 3
- 239000000427 antigen Substances 0.000 abstract 3
- 102000036639 antigens Human genes 0.000 abstract 3
- 108091007433 antigens Proteins 0.000 abstract 3
- AIJULSRZWUXGPQ-UHFFFAOYSA-N Methylglyoxal Chemical compound CC(=O)C=O AIJULSRZWUXGPQ-UHFFFAOYSA-N 0.000 abstract 2
- 239000000725 suspension Substances 0.000 abstract 2
- 239000005995 Aluminium silicate Substances 0.000 abstract 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 abstract 1
- 241000287828 Gallus gallus Species 0.000 abstract 1
- 108010010803 Gelatin Proteins 0.000 abstract 1
- 208000005647 Mumps Diseases 0.000 abstract 1
- 241000711386 Mumps virus Species 0.000 abstract 1
- 241000283973 Oryctolagus cuniculus Species 0.000 abstract 1
- 241000710799 Rubella virus Species 0.000 abstract 1
- 239000008351 acetate buffer Substances 0.000 abstract 1
- 235000012211 aluminium silicate Nutrition 0.000 abstract 1
- 229920000159 gelatin Polymers 0.000 abstract 1
- 239000008273 gelatin Substances 0.000 abstract 1
- 235000019322 gelatine Nutrition 0.000 abstract 1
- 235000011852 gelatine desserts Nutrition 0.000 abstract 1
- 206010022000 influenza Diseases 0.000 abstract 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 abstract 1
- 208000010805 mumps infectious disease Diseases 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 235000000346 sugar Nutrition 0.000 abstract 1
- 150000008163 sugars Chemical class 0.000 abstract 1
- 241000712461 unidentified influenza virus Species 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
- G01N33/555—Red blood cell
- G01N33/556—Fixed or stabilised red blood cell
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
1286857 Determination of virus antibodies ABBOTT LABORATORIES 29 Oct 1969 [31 Oct 1968] 53040/69 Heading B1X A test kit for use in the quantitative determination of a specific virus antibody in a serum comprises (a) a solution of an antigen specific to the antibody to be determined, (b) indicator cells in the form of a suspension of fixed red cells in a medium comprising an aqueous alkali metal acetate buffer pH 5.4 to 6.2, (c) a suspension of an absorbent in an alkali metal acetate-protein buffer pH 6.8 to 8.0, for removing non-specific agglutinants from the serum under test, and (d) an aqueous alkali metal acetate-protein buffer having a pH of 6.8 to 8.0. Antigens exemplified are influenza, mumps and rubella viruses. The cells may be chicken cells fixed by treatment with pyruvic aldehyde and formaldehyde as in Specification 1,244,344. The absorbent may be kaolin. The serum after treatment with the absorbent may be treated with fixed cells suspended in the buffer (d) before use in the test. The buffer may contain as protein rabbit serum albumin, bovine serum albumin, gelatin or normal human serum, and also salts and sugars (both exemplified). In carrying out the test, the antigen (a) diluted with buffer (d) is added to the pretreated serum sample and the fixed cells (c) are added.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US77239868A | 1968-10-31 | 1968-10-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1286857A true GB1286857A (en) | 1972-08-23 |
Family
ID=25094933
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB5304069A Expired GB1286857A (en) | 1968-10-31 | 1969-10-29 | Test kit for determining virus antibody |
Country Status (4)
| Country | Link |
|---|---|
| JP (1) | JPS4946050B1 (en) |
| CA (1) | CA943066A (en) |
| DE (1) | DE1954728C3 (en) |
| GB (1) | GB1286857A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4403037A (en) | 1980-10-10 | 1983-09-06 | American Hoechst Corporation | Erythrocyte preparations and use thereof in hemagglutination tests |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5289462U (en) * | 1975-12-27 | 1977-07-04 | ||
| JPS61142466A (en) * | 1984-12-14 | 1986-06-30 | Shionogi & Co Ltd | Stabilization of rubella ha antigen |
| CN104730259A (en) * | 2015-03-12 | 2015-06-24 | 青岛易邦生物工程有限公司 | Method for detecting hemagglutination inhibition antibody of chicken infectious bronchitis |
-
1969
- 1969-09-29 CA CA063,391A patent/CA943066A/en not_active Expired
- 1969-10-29 GB GB5304069A patent/GB1286857A/en not_active Expired
- 1969-10-30 DE DE19691954728 patent/DE1954728C3/en not_active Expired
- 1969-10-31 JP JP8695669A patent/JPS4946050B1/ja active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4403037A (en) | 1980-10-10 | 1983-09-06 | American Hoechst Corporation | Erythrocyte preparations and use thereof in hemagglutination tests |
Also Published As
| Publication number | Publication date |
|---|---|
| DE1954728A1 (en) | 1970-07-16 |
| DE1954728C3 (en) | 1978-12-07 |
| JPS4946050B1 (en) | 1974-12-07 |
| CA943066A (en) | 1974-03-05 |
| DE1954728B2 (en) | 1978-04-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PCNP | Patent ceased through non-payment of renewal fee |