GB1119668A - Method of testing microbial sensitivity to drugs - Google Patents
Method of testing microbial sensitivity to drugsInfo
- Publication number
- GB1119668A GB1119668A GB3678165A GB3678165A GB1119668A GB 1119668 A GB1119668 A GB 1119668A GB 3678165 A GB3678165 A GB 3678165A GB 3678165 A GB3678165 A GB 3678165A GB 1119668 A GB1119668 A GB 1119668A
- Authority
- GB
- United Kingdom
- Prior art keywords
- drug
- drugs
- measured
- culture medium
- dish
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 229940079593 drug Drugs 0.000 title abstract 11
- 239000003814 drug Substances 0.000 title abstract 11
- 230000000813 microbial effect Effects 0.000 title abstract 2
- 230000035945 sensitivity Effects 0.000 title abstract 2
- 238000010998 test method Methods 0.000 title abstract 2
- 239000001963 growth medium Substances 0.000 abstract 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract 3
- 239000002609 medium Substances 0.000 abstract 2
- 229920003023 plastic Polymers 0.000 abstract 2
- 239000004033 plastic Substances 0.000 abstract 2
- 229920001817 Agar Polymers 0.000 abstract 1
- 229930182555 Penicillin Natural products 0.000 abstract 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 abstract 1
- 108010040201 Polymyxins Proteins 0.000 abstract 1
- 230000002745 absorbent Effects 0.000 abstract 1
- 239000002250 absorbent Substances 0.000 abstract 1
- 239000008272 agar Substances 0.000 abstract 1
- 238000000151 deposition Methods 0.000 abstract 1
- 238000010410 dusting Methods 0.000 abstract 1
- 238000009630 liquid culture Methods 0.000 abstract 1
- 238000009629 microbiological culture Methods 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 229940049954 penicillin Drugs 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A method of testing microbial sensitivity to drug involves laying a measured layer of culture medium over a measured quantity of the drug (or mixture of drugs), allowing the drug to attain a uniform concentration in the plane part of the free surface of the medium, depositing a microbial culture on the solid medium surface and recording any growth after a suitable time and/or comparing it with any growth occurring on a comparable control layer of culture medium prepared without the drug. The several embodiments include the following features: (1) Discs of filter paper containing measured quantities of drug are each placed at the bottom of a flat-bottomed test-tube and are covered by a small, measured amount of culture medium. (2) There is employed a disposable Petri dish with several cylindrical cups sealed to its bottom into which the drug discs are placed. A measured volume of agar is dispensed into each cup, and the names and concentrations of the drugs are printed on a mat of absorbent blotting paper covering the bottom of the dish and surrounding the cups. The dish is vacuum sealed into a dark plastic bag, which is opened for use when required. According to a modification, solutions of drugs are evaporated to dryness in conical depressions in a sterile plastics plate, a trace of glycerol being added to avoid any dusting of the dried drug, and for use the depressions are filled with liquid culture medium containing the micro-organism. Reference is made to the higher diffusibility of penicillin than polymyxin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB3678165A GB1119668A (en) | 1965-08-26 | 1965-08-26 | Method of testing microbial sensitivity to drugs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB3678165A GB1119668A (en) | 1965-08-26 | 1965-08-26 | Method of testing microbial sensitivity to drugs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1119668A true GB1119668A (en) | 1968-07-10 |
Family
ID=10391139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB3678165A Expired GB1119668A (en) | 1965-08-26 | 1965-08-26 | Method of testing microbial sensitivity to drugs |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB1119668A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2204120C2 (en) * | 2001-06-07 | 2003-05-10 | Оленин Олег Дмитриевич | Device to take samples of microbic aerosols |
| RU2237236C1 (en) * | 2003-04-01 | 2004-09-27 | Оленин Олег Дмитриевич | Three-cascade impactor device for sampling microbial aerosols |
| US7951913B2 (en) | 2006-06-02 | 2011-05-31 | Biotika A.S. | Method of polymyxin B recovery from fermentation broth |
| US8119371B2 (en) | 2006-06-15 | 2012-02-21 | Biotika A.S. | Process for the preparation of polymyxin B employing (PAENI) Bacillus polymyxa |
| CN105255718A (en) * | 2015-10-16 | 2016-01-20 | 广东海大集团股份有限公司 | Testing cotton swab as well as preparation method and testing method thereof |
-
1965
- 1965-08-26 GB GB3678165A patent/GB1119668A/en not_active Expired
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2204120C2 (en) * | 2001-06-07 | 2003-05-10 | Оленин Олег Дмитриевич | Device to take samples of microbic aerosols |
| RU2237236C1 (en) * | 2003-04-01 | 2004-09-27 | Оленин Олег Дмитриевич | Three-cascade impactor device for sampling microbial aerosols |
| US7951913B2 (en) | 2006-06-02 | 2011-05-31 | Biotika A.S. | Method of polymyxin B recovery from fermentation broth |
| US8119371B2 (en) | 2006-06-15 | 2012-02-21 | Biotika A.S. | Process for the preparation of polymyxin B employing (PAENI) Bacillus polymyxa |
| CN105255718A (en) * | 2015-10-16 | 2016-01-20 | 广东海大集团股份有限公司 | Testing cotton swab as well as preparation method and testing method thereof |
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