GB1027979A - Process for obtaining bcg-cultures - Google Patents
Process for obtaining bcg-culturesInfo
- Publication number
- GB1027979A GB1027979A GB2597364A GB2597364A GB1027979A GB 1027979 A GB1027979 A GB 1027979A GB 2597364 A GB2597364 A GB 2597364A GB 2597364 A GB2597364 A GB 2597364A GB 1027979 A GB1027979 A GB 1027979A
- Authority
- GB
- United Kingdom
- Prior art keywords
- bcg
- nutrient
- trade mark
- registered trade
- cultures
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title abstract 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract 6
- 235000015097 nutrients Nutrition 0.000 abstract 5
- 239000000243 solution Substances 0.000 abstract 4
- 238000004113 cell culture Methods 0.000 abstract 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 2
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 abstract 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 abstract 2
- 229920000136 polysorbate Polymers 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- 230000004083 survival effect Effects 0.000 abstract 2
- 102000009027 Albumins Human genes 0.000 abstract 1
- 108010088751 Albumins Proteins 0.000 abstract 1
- -1 Bacto-Casiton Chemical compound 0.000 abstract 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 abstract 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 abstract 1
- 238000005273 aeration Methods 0.000 abstract 1
- FRHBOQMZUOWXQL-UHFFFAOYSA-L ammonium ferric citrate Chemical compound [NH4+].[Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FRHBOQMZUOWXQL-UHFFFAOYSA-L 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 229960001230 asparagine Drugs 0.000 abstract 1
- 239000001110 calcium chloride Substances 0.000 abstract 1
- 229910001628 calcium chloride Inorganic materials 0.000 abstract 1
- 235000011148 calcium chloride Nutrition 0.000 abstract 1
- 229940041514 candida albicans extract Drugs 0.000 abstract 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 abstract 1
- 239000008367 deionised water Substances 0.000 abstract 1
- 229960004642 ferric ammonium citrate Drugs 0.000 abstract 1
- 239000004313 iron ammonium citrate Substances 0.000 abstract 1
- 235000000011 iron ammonium citrate Nutrition 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 abstract 1
- 235000019341 magnesium sulphate Nutrition 0.000 abstract 1
- 229910052757 nitrogen Inorganic materials 0.000 abstract 1
- 230000000644 propagated effect Effects 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 239000001632 sodium acetate Substances 0.000 abstract 1
- 235000017281 sodium acetate Nutrition 0.000 abstract 1
- 239000001488 sodium phosphate Substances 0.000 abstract 1
- 229910000162 sodium phosphate Inorganic materials 0.000 abstract 1
- 235000011008 sodium phosphates Nutrition 0.000 abstract 1
- 239000007858 starting material Substances 0.000 abstract 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 1
- 229960005486 vaccine Drugs 0.000 abstract 1
- 239000000080 wetting agent Substances 0.000 abstract 1
- 239000012138 yeast extract Substances 0.000 abstract 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 abstract 1
- 239000011686 zinc sulphate Substances 0.000 abstract 1
- 235000009529 zinc sulphate Nutrition 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/04—Mycobacterium, e.g. Mycobacterium tuberculosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Communicable Diseases (AREA)
- Pulmonology (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A process for obtaining BCG cultures comprises cultivating BCG under aerobic conditions in a nutrient solution containing glycerol, a source of nitrogen, inorganic salts and a wetting agent of the "TWEEN" (Registered Trade Mark) type. The aeration may be performed with 0.1 to 0.5 part by volume of air by volume of nutrient solution per minute. The BCG for cultivating in the said nutrient may be prepared by a process wherein a BCG culture is lyophilised, the lyophilisate is stored for a considerable time at the highest storage temperature of the vaccine, and then suspended in an aqueous solution, the solution plated on a solid nutrient medium in such a concentration that separate colonies grow, a few single cell colonies are propagated separately in a liquid medium, the single-cell cultures so obtained are lyophilised and in order to determine the rate of survival a few ampoules of each single-cell culture are plated on a solid medium and the single-cell cultures having the highest rate of survival are used as starting material. The nutrient medium comprises glycerol, "TWEEN" 80 (Registered Trade Mark), L-asparagine, Bacto-Casiton, potasium phosphate sodium phosphate, ferric ammonium citrate, magnesium sulphate, calcium chloride, zinc sulphate, copper sulphate and deionised water and albumin solution or sodium acetate and "Difco" (Registered Trade Mark) yeast extract.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH873663A CH465768A (en) | 1963-07-12 | 1963-07-12 | Process for obtaining BCG cultures |
| CH691364 | 1964-05-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1027979A true GB1027979A (en) | 1966-05-04 |
Family
ID=25700381
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB2597364A Expired GB1027979A (en) | 1963-07-12 | 1964-06-23 | Process for obtaining bcg-cultures |
Country Status (8)
| Country | Link |
|---|---|
| BE (1) | BE650404A (en) |
| BR (1) | BR6460789D0 (en) |
| DE (1) | DE1442075B2 (en) |
| DK (1) | DK106687C (en) |
| ES (1) | ES301910A1 (en) |
| GB (1) | GB1027979A (en) |
| NL (1) | NL6407893A (en) |
| SE (1) | SE333432B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0140543A3 (en) * | 1983-11-03 | 1988-03-02 | Becton, Dickinson And Company | Method and composition for enhancement of microorganism growth |
| WO2003049752A3 (en) * | 2001-12-11 | 2003-11-20 | Pasteur Institut | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
-
1964
- 1964-06-23 GB GB2597364A patent/GB1027979A/en not_active Expired
- 1964-07-07 DE DE19641442075 patent/DE1442075B2/en active Pending
- 1964-07-09 DK DK344864A patent/DK106687C/en active
- 1964-07-10 BE BE650404D patent/BE650404A/xx unknown
- 1964-07-10 BR BR16078964A patent/BR6460789D0/en unknown
- 1964-07-10 SE SE848864A patent/SE333432B/xx unknown
- 1964-07-10 NL NL6407893A patent/NL6407893A/xx unknown
- 1964-07-10 ES ES0301910A patent/ES301910A1/en not_active Expired
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0140543A3 (en) * | 1983-11-03 | 1988-03-02 | Becton, Dickinson And Company | Method and composition for enhancement of microorganism growth |
| WO2003049752A3 (en) * | 2001-12-11 | 2003-11-20 | Pasteur Institut | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
| JP2005516915A (en) * | 2001-12-11 | 2005-06-09 | インスティティ・パスツール | Gram-positive bacterial preparations for the treatment of diseases involving immune dysregulation |
| AU2002356387B2 (en) * | 2001-12-11 | 2007-09-13 | Institut National De La Sante Et De La Recherche Medicale | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
| AU2002356387C1 (en) * | 2001-12-11 | 2008-03-06 | Institut National De La Sante Et De La Recherche Medicale | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
| US7871627B2 (en) | 2001-12-11 | 2011-01-18 | Institut Pasteur | Gram positive bacteria preparations for the treatment of disease comprising an immune dysregulation |
| KR101048798B1 (en) * | 2001-12-11 | 2011-07-15 | 앵스티띠 나셔날 드 라 쌍뜨 에 드 라 르셰르슈 메디깔 | Gram-positive bacteria preparations for the treatment of diseases including immunomodulatory disorder |
| CN1602199B (en) * | 2001-12-11 | 2012-04-04 | 巴斯德研究院 | Preparations of gram-positive bacteria for the treatment of diseases involving immune disorders |
| EP2316466A3 (en) * | 2001-12-11 | 2012-04-11 | Institut Pasteur | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
| US8404250B2 (en) | 2001-12-11 | 2013-03-26 | Institut Pasteur | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
Also Published As
| Publication number | Publication date |
|---|---|
| ES301910A1 (en) | 1965-01-01 |
| NL6407893A (en) | 1965-01-13 |
| DK106687C (en) | 1967-03-06 |
| BE650404A (en) | 1965-01-11 |
| BR6460789D0 (en) | 1973-07-26 |
| DE1442075A1 (en) | 1968-11-21 |
| DE1442075B2 (en) | 1973-02-01 |
| SE333432B (en) | 1971-03-15 |
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