GB1070157A - Compositions for the determination of aldolase activity - Google Patents
Compositions for the determination of aldolase activityInfo
- Publication number
- GB1070157A GB1070157A GB2100465A GB2100465A GB1070157A GB 1070157 A GB1070157 A GB 1070157A GB 2100465 A GB2100465 A GB 2100465A GB 2100465 A GB2100465 A GB 2100465A GB 1070157 A GB1070157 A GB 1070157A
- Authority
- GB
- United Kingdom
- Prior art keywords
- dichloroindophenol
- diphosphate
- arsenate
- dinucleotide
- reduced
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000001390 Fructose-Bisphosphate Aldolase Human genes 0.000 title abstract 3
- 108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 title abstract 3
- RXGJTUSBYWCRBK-UHFFFAOYSA-M 5-methylphenazinium methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 RXGJTUSBYWCRBK-UHFFFAOYSA-M 0.000 abstract 3
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 abstract 3
- 229940000489 arsenate Drugs 0.000 abstract 3
- 239000001177 diphosphate Substances 0.000 abstract 3
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 abstract 3
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 abstract 3
- DJHGAFSJWGLOIV-UHFFFAOYSA-K Arsenate3- Chemical compound [O-][As]([O-])([O-])=O DJHGAFSJWGLOIV-UHFFFAOYSA-K 0.000 abstract 2
- SENPVEZBRZQVST-HISDBWNOSA-N deamido-NAD zwitterion Chemical compound [N+]1([C@@H]2O[C@@H]([C@H]([C@H]2O)O)COP([O-])(=O)OP(O)(=O)OC[C@H]2O[C@H]([C@@H]([C@@H]2O)O)N2C=3N=CN=C(C=3N=C2)N)=CC=CC(C(O)=O)=C1 SENPVEZBRZQVST-HISDBWNOSA-N 0.000 abstract 2
- 241001465754 Metazoa Species 0.000 abstract 1
- FBWADIKARMIWNM-UHFFFAOYSA-N N-3,5-dichloro-4-hydroxyphenyl-1,4-benzoquinone imine Chemical compound C1=C(Cl)C(O)=C(Cl)C=C1N=C1C=CC(=O)C=C1 FBWADIKARMIWNM-UHFFFAOYSA-N 0.000 abstract 1
- 229910052783 alkali metal Inorganic materials 0.000 abstract 1
- -1 alkali metal arsenate Chemical class 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 239000000975 dye Substances 0.000 abstract 1
- LXJXRIRHZLFYRP-UHFFFAOYSA-N glyceraldehyde 3-phosphate Chemical compound O=CC(O)COP(O)(O)=O LXJXRIRHZLFYRP-UHFFFAOYSA-N 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A composition for the determination of aldolase, e.g. in body liquids of animals and man, comprises fructose- 1:6-diphosphate, glyceraldehyde phosphate dehydrogenase, an alkali metal arsenate, nicotinic acid adenine dinucleotide, 2:6-dichloroindophenol, phenazine methosulphate and a triethanolamine buffer of pH 7-8.5. In the presence of aldolase, the fructose-1:6-diphosphate is hydrolysed to 3-phosphoglyceraldehyde and in the presence of glyceraldehyde phosphate dehydrogenase and arsenate, oxidation takes place, whilst the dinucleotide is reduced: the dyestuff system (2:6 -dichloroindophenol, phenazine methosulphate) is then reduced by the reduced dinucleotide. The materials may be put up as a test pack in the form of the following aqueous solutions:-(1) triethanolamine, nicotinic acid adenine dinucleotide, dichloroindophenol and arsenate, (2) phenazine methosulphate, (3) fructose- 1:6-diphosphate, (4) glyceraldehyde phosphate dehydrogenase and (5) triethanolamine buffer. Alternatively the composition may be impregnated in a suitable carrier, e.g. filter paper strips, and the product lyophilised.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB2100465A GB1070157A (en) | 1965-05-18 | 1965-05-18 | Compositions for the determination of aldolase activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB2100465A GB1070157A (en) | 1965-05-18 | 1965-05-18 | Compositions for the determination of aldolase activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1070157A true GB1070157A (en) | 1967-05-24 |
Family
ID=10155550
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB2100465A Expired GB1070157A (en) | 1965-05-18 | 1965-05-18 | Compositions for the determination of aldolase activity |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB1070157A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0054096A1 (en) * | 1980-12-12 | 1982-06-23 | Ivan Endre Modrovich | Method of forming stabilized urease solutions |
-
1965
- 1965-05-18 GB GB2100465A patent/GB1070157A/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0054096A1 (en) * | 1980-12-12 | 1982-06-23 | Ivan Endre Modrovich | Method of forming stabilized urease solutions |
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