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FR3118169B1 - Procédé de caractérisation de spermatozoïdes - Google Patents

Procédé de caractérisation de spermatozoïdes

Info

Publication number
FR3118169B1
FR3118169B1 FR2013979A FR2013979A FR3118169B1 FR 3118169 B1 FR3118169 B1 FR 3118169B1 FR 2013979 A FR2013979 A FR 2013979A FR 2013979 A FR2013979 A FR 2013979A FR 3118169 B1 FR3118169 B1 FR 3118169B1
Authority
FR
France
Prior art keywords
image
particle
images
series
resulting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR2013979A
Other languages
English (en)
Other versions
FR3118169A1 (fr
Inventor
Cédric Allier
Olivier Cioni
Ondrej Mandula
Agnès Camus
Eric Schmitt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Commissariat a lEnergie Atomique CEA
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commissariat a lEnergie Atomique CEA, Commissariat a lEnergie Atomique et aux Energies Alternatives CEA filed Critical Commissariat a lEnergie Atomique CEA
Priority to FR2013979A priority Critical patent/FR3118169B1/fr
Priority to EP21839575.4A priority patent/EP4268120A1/fr
Priority to US18/258,726 priority patent/US20240044771A1/en
Priority to PCT/EP2021/086909 priority patent/WO2022136325A1/fr
Publication of FR3118169A1 publication Critical patent/FR3118169A1/fr
Application granted granted Critical
Publication of FR3118169B1 publication Critical patent/FR3118169B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00—Arrangements for image or video recognition or understanding
    • G06V10/40—Extraction of image or video features
    • G06V10/44—Local feature extraction by analysis of parts of the pattern, e.g. by detecting edges, contours, loops, corners, strokes or intersections; Connectivity analysis, e.g. of connected components
    • G06V10/443—Local feature extraction by analysis of parts of the pattern, e.g. by detecting edges, contours, loops, corners, strokes or intersections; Connectivity analysis, e.g. of connected components by matching or filtering
    • G06V10/449—Biologically inspired filters, e.g. difference of Gaussians [DoG] or Gabor filters
    • G06V10/451—Biologically inspired filters, e.g. difference of Gaussians [DoG] or Gabor filters with interaction between the filter responses, e.g. cortical complex cells
    • G06V10/454—Integrating the filters into a hierarchical structure, e.g. convolutional neural networks [CNN]
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1027—Determining speed or velocity of a particle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/144—Imaging characterised by its optical setup
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1497—Particle shape

Landscapes

  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • Multimedia (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Artificial Intelligence (AREA)
  • Evolutionary Computation (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Signal Processing (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Image Analysis (AREA)
  • Image Processing (AREA)

Abstract

Procédé de caractérisation d’une particule mobile dans un échantillon (10), le procédé comportant: acquisition d’au moins une image () de l’échantillon durant une période d’acquisition, à l’aide d’un capteur d’image (20) et formation d’une série d’images, la série d’images comportant au moins une image;utilisation de chaque image de la série d’images résultant de a) en tant qu’image d’entrée () d’un réseau de neurones convolutif de détection (CNNd), le réseau de neurones convolutif de détection étant configuré pour détecter les particules et pour produire, à partir de chaque image, une image de sortie () sur laquelle à chaque particule détectée est assignée une distribution d’intensité, centrée sur la particule et s’étendant autour de la particule ;pour chaque particule détectée, à partir de chaque image de sortie () résultant de b), estimation d’une position de chaque particule détectée dans chaque image de la série d’images ; caractérisation de chaque particule détectée à partir de l’estimation de la position résultant de c), établie à partir de chaque image de la série d’images.
FR2013979A 2020-12-22 2020-12-22 Procédé de caractérisation de spermatozoïdes Active FR3118169B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR2013979A FR3118169B1 (fr) 2020-12-22 2020-12-22 Procédé de caractérisation de spermatozoïdes
EP21839575.4A EP4268120A1 (fr) 2020-12-22 2021-12-20 Procédé de caractérisation de spermatozoïdes
US18/258,726 US20240044771A1 (en) 2020-12-22 2021-12-20 Method for characterising sperm cells
PCT/EP2021/086909 WO2022136325A1 (fr) 2020-12-22 2021-12-20 Procédé de caractérisation de spermatozoïdes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2013979A FR3118169B1 (fr) 2020-12-22 2020-12-22 Procédé de caractérisation de spermatozoïdes
FR2013979 2020-12-22

Publications (2)

Publication Number Publication Date
FR3118169A1 FR3118169A1 (fr) 2022-06-24
FR3118169B1 true FR3118169B1 (fr) 2025-10-17

Family

ID=74592263

Family Applications (1)

Application Number Title Priority Date Filing Date
FR2013979A Active FR3118169B1 (fr) 2020-12-22 2020-12-22 Procédé de caractérisation de spermatozoïdes

Country Status (4)

Country Link
US (1) US20240044771A1 (fr)
EP (1) EP4268120A1 (fr)
FR (1) FR3118169B1 (fr)
WO (1) WO2022136325A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12475564B2 (en) * 2022-02-16 2025-11-18 Proscia Inc. Digital pathology artificial intelligence quality check
FR3154495B1 (fr) * 2023-10-23 2025-09-05 Commissariat Energie Atomique Procédé de détection de particules présentes dans un liquide
FR3164787A1 (fr) 2024-07-18 2026-01-23 Commissariat A L'energie Atomique Et Aux Energies Alternatives Procédé de caractérisation de la trajectoire d’une particule mobile dans un échantillon

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60129831T2 (de) * 2000-09-25 2008-04-30 Sensovation Ag Vorrichtung und Verfahren zur optischen Messung
US8842901B2 (en) 2010-12-14 2014-09-23 The Regents Of The University Of California Compact automated semen analysis platform using lens-free on-chip microscopy
US9588037B2 (en) 2012-07-13 2017-03-07 The Regents Of The University Of California High throughput lens-free three-dimensional tracking of sperm
FR3028951B1 (fr) 2014-11-21 2017-01-06 Commissariat Energie Atomique Systeme d'imagerie sans lentille comprenant une diode, un diaphragme et un diffuseur entre la diode et le diaphragme
FR3030749B1 (fr) * 2014-12-19 2020-01-03 Commissariat A L'energie Atomique Et Aux Energies Alternatives Methode d'identification de particules biologiques par piles d'images holographiques defocalisees
FR3034197B1 (fr) 2015-03-24 2020-05-01 Commissariat A L'energie Atomique Et Aux Energies Alternatives Procede de determination de l'etat d'une cellule
FR3034196B1 (fr) 2015-03-24 2019-05-31 Commissariat A L'energie Atomique Et Aux Energies Alternatives Procede d'analyse de particules
FR3036800B1 (fr) 2015-05-28 2020-02-28 Commissariat A L'energie Atomique Et Aux Energies Alternatives Procede d’observation d’un echantillon
FR3049347B1 (fr) 2016-03-23 2018-04-27 Commissariat A L'energie Atomique Et Aux Energies Alternatives Procede d’observation d’un echantillon par calcul d’une image complexe
FR3075372B1 (fr) 2017-12-18 2020-08-28 Commissariat Energie Atomique Dispositif et procede d'observation d'un echantillon avec un systeme optique chromatique
US10094759B1 (en) * 2017-12-22 2018-10-09 Hillel Llc Imaging device for measuring sperm motility
FR3081552B1 (fr) * 2018-05-23 2020-05-29 Commissariat A L'energie Atomique Et Aux Energies Alternatives Dispositif et procede d'observation d'un echantillon fluorescent par imagerie defocalisee
FR3087538B1 (fr) 2018-10-17 2020-10-09 Commissariat Energie Atomique Procede d'observation d'un echantillon

Also Published As

Publication number Publication date
FR3118169A1 (fr) 2022-06-24
WO2022136325A1 (fr) 2022-06-30
US20240044771A1 (en) 2024-02-08
EP4268120A1 (fr) 2023-11-01

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