[go: up one dir, main page]

FR3091295B1 - Systeme fluidique de production de vesicules extracellulaires et procede associe - Google Patents

Systeme fluidique de production de vesicules extracellulaires et procede associe Download PDF

Info

Publication number
FR3091295B1
FR3091295B1 FR1874295A FR1874295A FR3091295B1 FR 3091295 B1 FR3091295 B1 FR 3091295B1 FR 1874295 A FR1874295 A FR 1874295A FR 1874295 A FR1874295 A FR 1874295A FR 3091295 B1 FR3091295 B1 FR 3091295B1
Authority
FR
France
Prior art keywords
production
producer cells
suspension
liquid medium
agitator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1874295A
Other languages
English (en)
Other versions
FR3091295A1 (fr
Inventor
Alice Grangier
Amanda Silva
Florence Gazeau
Claire Wilhelm
Max Piffoux
François Heslot
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Universite Paris Cite
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite Paris Diderot Paris 7
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR1874295A priority Critical patent/FR3091295B1/fr
Application filed by Centre National de la Recherche Scientifique CNRS, Universite Paris Diderot Paris 7 filed Critical Centre National de la Recherche Scientifique CNRS
Priority to MX2021007776A priority patent/MX2021007776A/es
Priority to US17/418,970 priority patent/US20220119748A1/en
Priority to JP2021537118A priority patent/JP7621952B2/ja
Priority to EP19850769.1A priority patent/EP3902904A1/fr
Priority to CN201980093099.5A priority patent/CN113748198B/zh
Priority to CA3124605A priority patent/CA3124605A1/fr
Priority to KR1020217023047A priority patent/KR20210134611A/ko
Priority to PCT/FR2019/053309 priority patent/WO2020136362A1/fr
Publication of FR3091295A1 publication Critical patent/FR3091295A1/fr
Application granted granted Critical
Publication of FR3091295B1 publication Critical patent/FR3091295B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26—Conditioning fluids entering or exiting the reaction vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0635—B lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0063—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres
    • A61K49/0069—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres the agent being in a particular physical galenical form
    • A61K49/0097—Cells, viruses, ghosts, red blood cells, viral vectors, used for imaging or diagnosis in vivo
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Materials For Medical Uses (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Radiology & Medical Imaging (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Developmental Biology & Embryology (AREA)
  • Medicinal Chemistry (AREA)

Abstract

L’invention concerne un système fluidique de production de vésicules extracellulaires (EV) à partir de cellules productrices en suspension, comprenant au moins un récipient, un milieu liquide contenu par le récipient, des cellules productrices en suspension, un agitateur de milieu liquide, des moyens de commande de la vitesse de l’agitateur adaptés pour la croissance des cellules productrices en suspension, caractérisé en ce qu’il comprend également des moyens de commande de la vitesse de l’agitateur et un agitateur dont la forme et les dimensions du récipient sont adaptés à la génération d’un écoulement turbulent du milieu liquide dans le récipient pour exercer des contraintes de cisaillement sur les cellules productrices afin de réaliser la production de vésicules extracellulaires (EV), la longueur de Kolmogorov de l’écoulement étant inférieure ou égale à 50 µm. Figure à publier avec l’abrégé : Figure 1
FR1874295A 2018-12-28 2018-12-28 Systeme fluidique de production de vesicules extracellulaires et procede associe Active FR3091295B1 (fr)

Priority Applications (9)

Application Number Priority Date Filing Date Title
FR1874295A FR3091295B1 (fr) 2018-12-28 2018-12-28 Systeme fluidique de production de vesicules extracellulaires et procede associe
US17/418,970 US20220119748A1 (en) 2018-12-28 2019-12-27 Fluidic system for producing extracellular vesicles and associated method
JP2021537118A JP7621952B2 (ja) 2018-12-28 2019-12-27 細胞外小胞を製造するための流体系および関連方法
EP19850769.1A EP3902904A1 (fr) 2018-12-28 2019-12-27 Système fluidique de production de vésicules extracellulaires et procédé associé
MX2021007776A MX2021007776A (es) 2018-12-28 2019-12-27 Sistema fluidico para la producciön de vesïculas extracelulares y mëtodo asociado.
CN201980093099.5A CN113748198B (zh) 2018-12-28 2019-12-27 用于生产细胞外囊泡的流体系统及相关方法
CA3124605A CA3124605A1 (fr) 2018-12-28 2019-12-27 Systeme fluidique de production de vesicules extracellulaires et procede associe
KR1020217023047A KR20210134611A (ko) 2018-12-28 2019-12-27 세포외 소낭의 생성을 위한 유체 시스템 및 연관된 방법
PCT/FR2019/053309 WO2020136362A1 (fr) 2018-12-28 2019-12-27 Système fluidique de production de vésicules extracellulaires et procédé associé

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1874295A FR3091295B1 (fr) 2018-12-28 2018-12-28 Systeme fluidique de production de vesicules extracellulaires et procede associe

Publications (2)

Publication Number Publication Date
FR3091295A1 FR3091295A1 (fr) 2020-07-03
FR3091295B1 true FR3091295B1 (fr) 2023-05-26

Family

ID=67001928

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1874295A Active FR3091295B1 (fr) 2018-12-28 2018-12-28 Systeme fluidique de production de vesicules extracellulaires et procede associe

Country Status (9)

Country Link
US (1) US20220119748A1 (fr)
EP (1) EP3902904A1 (fr)
JP (1) JP7621952B2 (fr)
KR (1) KR20210134611A (fr)
CN (1) CN113748198B (fr)
CA (1) CA3124605A1 (fr)
FR (1) FR3091295B1 (fr)
MX (1) MX2021007776A (fr)
WO (1) WO2020136362A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3091296B1 (fr) * 2018-12-28 2021-02-19 Univ De Lorraine Systeme fluidique de production de vesicules extracellulaires comprenant un agent therapeutique ou d’imagerie et procede associe
CN112458055B (zh) * 2020-11-24 2023-12-29 重庆大学 一种基于切应力刺激作用下制备细胞微囊泡的方法
WO2022136226A1 (fr) 2020-12-21 2022-06-30 INSERM (Institut National de la Santé et de la Recherche Médicale) Composition de miarn comprenant 11 miarn spécifiques et son utilisation dans le traitement du cancer
WO2024200570A2 (fr) 2023-03-28 2024-10-03 Institut Curie Procédé de production à haut débit de vésicules extracellulaires dans un récipient rotatif à déflecteur
WO2025155498A1 (fr) * 2024-01-18 2025-07-24 University Of Iowa Research Foundation Appareil et procédé pour isoler des vésicules extracellulaires d'une suspension liquide par exposition de cellules à une contrainte de cisaillement de fluide
WO2025176642A1 (fr) 2024-02-19 2025-08-28 Revatis S.a. Procédé de préparation d'une composition de vésicule extracellulaire, composition de vésicule extracellulaire et utilisation associée
WO2025186182A1 (fr) 2024-03-04 2025-09-12 Institut Curie Production à haut rendement de vésicules extracellulaires dans une puce fluidique

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2870348B1 (fr) * 2004-05-14 2010-08-27 Univ Angers Methode pour diagnostiquer la presence et/ou la severite d'une pathologie hepathique chez un sujet
JP2006296423A (ja) * 2005-03-24 2006-11-02 Hitachi Ltd 培養槽の制御装置及び培養装置
US7771988B2 (en) * 2005-03-24 2010-08-10 Hitachi, Ltd. Control device for fermenter
US9567559B2 (en) * 2012-03-15 2017-02-14 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
JP5913084B2 (ja) * 2012-12-26 2016-04-27 株式会社日立製作所 培養制御方法、細胞培養装置及び細胞特性評価装置
WO2016203414A1 (fr) * 2015-06-16 2016-12-22 Fondazione Città Della Speranza - Onlus Vésicules extracellulaires issues de cellules de lignée ostéoblastique, à usage thérapeutique et diagnostique
JP2019501179A (ja) * 2015-12-30 2019-01-17 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 細胞由来ベシクルの向上された生成および単離のための方法
EP3452575A4 (fr) * 2016-05-05 2020-03-11 Terumo BCT, Inc. Production et collecte automatisés
EP3548003A4 (fr) * 2016-11-30 2020-07-22 The Regents of The University of California Vésicules extracellulaires et leurs procédés et utilisations
FR3068361B1 (fr) * 2017-06-30 2021-10-15 Univ Paris Diderot Paris 7 Systeme fluidique de production de vesicules extracellulaires et procede associe

Also Published As

Publication number Publication date
CA3124605A1 (fr) 2020-07-02
US20220119748A1 (en) 2022-04-21
KR20210134611A (ko) 2021-11-10
JP2022515269A (ja) 2022-02-17
EP3902904A1 (fr) 2021-11-03
JP7621952B2 (ja) 2025-01-27
CN113748198B (zh) 2024-04-12
MX2021007776A (es) 2021-10-13
FR3091295A1 (fr) 2020-07-03
WO2020136362A1 (fr) 2020-07-02
CN113748198A (zh) 2021-12-03

Similar Documents

Publication Publication Date Title
FR3091295B1 (fr) Systeme fluidique de production de vesicules extracellulaires et procede associe
FR3068361B1 (fr) Systeme fluidique de production de vesicules extracellulaires et procede associe
FR3091296B1 (fr) Systeme fluidique de production de vesicules extracellulaires comprenant un agent therapeutique ou d’imagerie et procede associe
ZA200903060B (en) An inventory system for liquids dispensed from a container
WO2012120101A4 (fr) Appareil et procédés de traitement de liquides ou de substances à base de liquide
Blanpain-Avet et al. The effect of oscillatory flow on crossflow microfiltration of beer in a tubular mineral membrane system–Membrane fouling resistance decrease and energetic considerations
JP2017535425A (ja) 気体を液体に溶解するための装置
FR3027678B1 (fr) Procede de detection d'organismes dans un echantillon dilue
Kaminker et al. The dynamics of open precipitation tubes
ATE503578T1 (de) Verwendung einer vorrichtung zur untersuchung von probenflüssigkeit
Artemi et al. The relevance of critical flux concept in the concentration of skim milk using forward osmosis and reverse osmosis
Xiao et al. Microscale insights into enzyme-induced carbonate precipitation in rock-based microfluidic chips
CN105878029A (zh) 一种无缝胶丸的制备方法
TW200724893A (en) Hydraulic device for a blood analysis apparatus, associated method and analysis apparatus equipped with such a device
RU2473388C1 (ru) Лабораторный модуль
CN205774404U (zh) 一种自动控制的茶油脱色装置
CN202415421U (zh) 简易蛋白质动态透析系统
FR2785598B1 (fr) Procede pour remplir avec precision un recipient avec un liquide et dispositif pour la mise en oeuvre
MY172254A (en) High temperature drilling with lower temperature drated tools
WO2004058935A3 (fr) Dispositif et procede de mise en culture parallele automatisee de cellules dans des conditions techniques
RU164038U1 (ru) Лабораторная установка для моделирования гидродинамики металлического расплава
FR2873928B1 (fr) Machine et procede de melange en continu et selon un ratio determine d'un fluide biologique et d'une solution
CN103881898B (zh) 滴灌系统管壁附生生物膜培养装置及其使用方法
FR3074297B1 (fr) Systeme et procede de mesure de l'erosion d'un sol
FR3068360B1 (fr) Procede d'analyse et de culture cellulaire et systeme associe

Legal Events

Date Code Title Description
PLFP Fee payment

Year of fee payment: 2

PLSC Publication of the preliminary search report

Effective date: 20200703

PLFP Fee payment

Year of fee payment: 3

TQ Partial transmission of property

Owner name: UNIVERSITE DE PARIS, FR

Effective date: 20201127

Owner name: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, FR

Effective date: 20201127

PLFP Fee payment

Year of fee payment: 4

CD Change of name or company name

Owner name: UNIVERSITE PARIS CITE, FR

Effective date: 20221006

Owner name: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, FR

Effective date: 20221006

PLFP Fee payment

Year of fee payment: 5

PLFP Fee payment

Year of fee payment: 6

PLFP Fee payment

Year of fee payment: 7

PLFP Fee payment

Year of fee payment: 8