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FR3062395B1 - Microorganisme genetiquement optimise pour la production de molecules d'interet - Google Patents

Microorganisme genetiquement optimise pour la production de molecules d'interet Download PDF

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Publication number
FR3062395B1
FR3062395B1 FR1750696A FR1750696A FR3062395B1 FR 3062395 B1 FR3062395 B1 FR 3062395B1 FR 1750696 A FR1750696 A FR 1750696A FR 1750696 A FR1750696 A FR 1750696A FR 3062395 B1 FR3062395 B1 FR 3062395B1
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FR
France
Prior art keywords
interest
molecules
production
microorganism
genetically modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR1750696A
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English (en)
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FR3062395A1 (fr
Inventor
Cedric Boisart
Nicolas Morin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enobraq
Original Assignee
Enobraq
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR1750696A priority Critical patent/FR3062395B1/fr
Application filed by Enobraq filed Critical Enobraq
Priority to US16/480,579 priority patent/US20190359988A1/en
Priority to BR112019015411-6A priority patent/BR112019015411A2/pt
Priority to KR1020197022622A priority patent/KR20190114982A/ko
Priority to JP2019561348A priority patent/JP2020506722A/ja
Priority to PCT/EP2018/052004 priority patent/WO2018138288A1/fr
Priority to EP18702646.3A priority patent/EP3574082A1/fr
Priority to CN201880019539.8A priority patent/CN110475851A/zh
Publication of FR3062395A1 publication Critical patent/FR3062395A1/fr
Application granted granted Critical
Publication of FR3062395B1 publication Critical patent/FR3062395B1/fr
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01001—Transketolase (2.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01002—Transaldolase (2.2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01019—Phosphoribulokinase (2.7.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01039—Ribulose-bisphosphate carboxylase (4.1.1.39)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un microorganisme génétiquement modifié exprimant une enzyme RuBisCO de type I ou II et une phosphoribulokinase (PRK) fonctionnelles, et dans lequel la branche non-oxydative de la voie des pentoses phosphates est au moins partiellement inhibée, ledit microorganisme étant génétiquement modifié de manière à produire une molécule exogène et/ou à surproduire une molécule endogène. L'invention concerne également l'utilisation d'un tel microorganisme génétiquement modifié, pour la production ou la surproduction d'une molécule d'intérêt et des procédés de synthèse ou bioconversion de molécules d'intérêt.
FR1750696A 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet Expired - Fee Related FR3062395B1 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
FR1750696A FR3062395B1 (fr) 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet
BR112019015411-6A BR112019015411A2 (pt) 2017-01-27 2018-01-26 Microorganismo geneticamente otimizado para produzir moléculas de interesse
KR1020197022622A KR20190114982A (ko) 2017-01-27 2018-01-26 대상 분자의 생산을 위한 유전자 최적화 미생물
JP2019561348A JP2020506722A (ja) 2017-01-27 2018-01-26 目的分子を産生するための遺伝的に最適化された微生物
US16/480,579 US20190359988A1 (en) 2017-01-27 2018-01-26 Genetically optimised microorganism for producing molecules of interest
PCT/EP2018/052004 WO2018138288A1 (fr) 2017-01-27 2018-01-26 Microorganisme génétiquement optimisé pour la production de molécules d'intérêt
EP18702646.3A EP3574082A1 (fr) 2017-01-27 2018-01-26 Microorganisme génétiquement optimisé pour la production de molécules d'intérêt
CN201880019539.8A CN110475851A (zh) 2017-01-27 2018-01-26 用于产生感兴趣的分子的经基因优化的微生物

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1750696 2017-01-27
FR1750696A FR3062395B1 (fr) 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet

Publications (2)

Publication Number Publication Date
FR3062395A1 FR3062395A1 (fr) 2018-08-03
FR3062395B1 true FR3062395B1 (fr) 2021-04-16

Family

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Family Applications (1)

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FR1750696A Expired - Fee Related FR3062395B1 (fr) 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet

Country Status (8)

Country Link
US (1) US20190359988A1 (fr)
EP (1) EP3574082A1 (fr)
JP (1) JP2020506722A (fr)
KR (1) KR20190114982A (fr)
CN (1) CN110475851A (fr)
BR (1) BR112019015411A2 (fr)
FR (1) FR3062395B1 (fr)
WO (1) WO2018138288A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105209598A (zh) * 2013-02-22 2015-12-30 代尔夫特理工大学 用于产量增加的方法中的重组体微生物
US11767521B2 (en) * 2020-02-21 2023-09-26 The Regents Of The University Of California Genetically modified bacterial cells and methods useful for producing indigoidine
CN118956840A (zh) * 2021-04-19 2024-11-15 武汉合生科技有限公司 获得α-法尼烯以及β-法尼烯的重组微生物及其构建方法
CN115259391B (zh) * 2022-08-26 2023-10-10 辽宁中森生物科技有限公司 一种污水处理用多元菌藻组合物、复合剂及其应用
CN117210446A (zh) * 2023-03-21 2023-12-12 北京蓝晶微生物科技有限公司 一种重建卡尔文循环提高异养微生物代谢产物产量的方法
CN117721054B (zh) * 2023-12-12 2025-01-28 四川大学 一种提高乳酸菌在盐胁迫下生物膜产量的方法
CN119709580A (zh) * 2025-03-04 2025-03-28 诸城市浩天药业有限公司 一种高效生产d-泛酸的工程菌株及其构建方法与应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2732031T3 (pl) * 2011-07-12 2016-03-31 Scientist Of Fortune Sa Rekombinowany mikroorganizm do wytwarzania użytecznych metabolitów
FR3016371B1 (fr) * 2014-01-16 2018-02-02 Institut National De La Recherche Agronomique Levures modifiees pour utiliser le dioxyde de carbone

Also Published As

Publication number Publication date
EP3574082A1 (fr) 2019-12-04
US20190359988A1 (en) 2019-11-28
FR3062395A1 (fr) 2018-08-03
KR20190114982A (ko) 2019-10-10
BR112019015411A2 (pt) 2020-05-26
JP2020506722A (ja) 2020-03-05
WO2018138288A1 (fr) 2018-08-02
CN110475851A (zh) 2019-11-19

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